[M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 1036823, 29, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (393, 481, 960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2094) [M::mem_pestat] mean and std.dev: (587.90, 349.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1395, 2571, 4208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9834) [M::mem_pestat] mean and std.dev: (3039.00, 2492.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13009) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1651, 2305, 4284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9550) [M::mem_pestat] mean and std.dev: (2640.08, 1400.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12183) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1055.338 CPU sec, 70.418 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 1036179, 32, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (378, 856, 1445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3579) [M::mem_pestat] mean and std.dev: (932.10, 835.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (297, 1928, 3615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10251) [M::mem_pestat] mean and std.dev: (2432.53, 2492.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13569) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (458, 1047, 1956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4952) [M::mem_pestat] mean and std.dev: (1373.82, 1187.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6450) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1043.317 CPU sec, 48.441 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1035734, 32, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (377, 659, 1078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2480) [M::mem_pestat] mean and std.dev: (718.35, 443.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3181) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.05, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (746, 2524, 4861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13091) [M::mem_pestat] mean and std.dev: (3128.16, 3175.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17206) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (893, 1040, 4341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11237) [M::mem_pestat] mean and std.dev: (2376.26, 2395.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14685) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1034.531 CPU sec, 51.178 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036564, 34, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (482, 1291, 2447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6377) [M::mem_pestat] mean and std.dev: (1638.00, 1473.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8342) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (400, 2590, 7868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22804) [M::mem_pestat] mean and std.dev: (3395.44, 3334.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 30272) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (880, 1575, 2716) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6388) [M::mem_pestat] mean and std.dev: (1542.08, 922.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8224) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1030.628 CPU sec, 45.362 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036296, 31, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (717, 1632, 3997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10557) [M::mem_pestat] mean and std.dev: (2338.00, 1966.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (708, 2279, 5220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14244) [M::mem_pestat] mean and std.dev: (2910.84, 2702.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18756) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (940, 2476, 5964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16012) [M::mem_pestat] mean and std.dev: (3485.42, 3104.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21036) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1025.883 CPU sec, 50.370 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036501, 38, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (363, 890, 2074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5496) [M::mem_pestat] mean and std.dev: (1070.76, 923.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7207) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.88) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (248, 1554, 3605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10319) [M::mem_pestat] mean and std.dev: (2277.29, 2526.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13676) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (485, 821, 2431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6323) [M::mem_pestat] mean and std.dev: (1166.07, 971.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8269) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1025.279 CPU sec, 45.149 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036943, 34, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 718, 1565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4019) [M::mem_pestat] mean and std.dev: (802.12, 550.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5246) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (367, 1882, 2608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7090) [M::mem_pestat] mean and std.dev: (1605.74, 1397.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9331) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (676, 1025, 1758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3922) [M::mem_pestat] mean and std.dev: (1094.47, 647.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5004) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1039.964 CPU sec, 68.504 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1036613, 33, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (398, 780, 1367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3305) [M::mem_pestat] mean and std.dev: (736.88, 485.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4274) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.82) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (757, 2587, 5375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14611) [M::mem_pestat] mean and std.dev: (3180.70, 2903.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19229) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (406, 923, 1446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3526) [M::mem_pestat] mean and std.dev: (847.00, 580.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4566) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1025.673 CPU sec, 47.591 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1035788, 32, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (357, 471, 970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2196) [M::mem_pestat] mean and std.dev: (611.70, 545.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (223, 1374, 3441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9877) [M::mem_pestat] mean and std.dev: (2457.34, 3055.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14679) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (910, 1301, 2325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5155) [M::mem_pestat] mean and std.dev: (1536.23, 1086.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6570) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1027.408 CPU sec, 51.537 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 1035374, 41, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (449, 1117, 2143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5531) [M::mem_pestat] mean and std.dev: (1356.29, 1239.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7225) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (166, 1855, 3435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9973) [M::mem_pestat] mean and std.dev: (2424.90, 2696.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13242) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (531, 1262, 2739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7155) [M::mem_pestat] mean and std.dev: (1715.50, 1360.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9363) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1020.422 CPU sec, 44.973 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036608, 41, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (914, 1369, 2788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6536) [M::mem_pestat] mean and std.dev: (1448.44, 864.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (174, 898, 3599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10449) [M::mem_pestat] mean and std.dev: (2198.05, 2670.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13874) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1428, 1797, 4871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11757) [M::mem_pestat] mean and std.dev: (2511.08, 1617.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15200) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1021.907 CPU sec, 48.294 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1036225, 38, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (365, 881, 2568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6974) [M::mem_pestat] mean and std.dev: (1571.40, 1688.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9177) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (293, 2068, 2952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8270) [M::mem_pestat] mean and std.dev: (1837.89, 1635.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10929) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (853, 1190, 2008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4318) [M::mem_pestat] mean and std.dev: (1405.58, 704.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5473) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1021.821 CPU sec, 45.053 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036080, 32, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (426, 1687, 7757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22419) [M::mem_pestat] mean and std.dev: (3351.71, 3382.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 29750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (278, 2516, 5536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16052) [M::mem_pestat] mean and std.dev: (3018.00, 2983.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21310) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (771, 1384, 2737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6669) [M::mem_pestat] mean and std.dev: (1705.09, 1381.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8635) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1037.977 CPU sec, 66.482 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036416, 37, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (373, 1274, 2569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6961) [M::mem_pestat] mean and std.dev: (1276.33, 1363.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9157) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (750, 2593, 8503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24009) [M::mem_pestat] mean and std.dev: (3833.24, 3489.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 31762) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1456, 1702, 3925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8863) [M::mem_pestat] mean and std.dev: (2608.54, 2061.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11332) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1026.517 CPU sec, 47.702 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1036097, 27, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (401, 1036, 1840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4718) [M::mem_pestat] mean and std.dev: (964.72, 756.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6157) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (792, 3570, 8308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 23340) [M::mem_pestat] mean and std.dev: (3696.59, 3125.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 30856) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (499, 1275, 2136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5410) [M::mem_pestat] mean and std.dev: (1338.42, 1134.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7047) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1024.539 CPU sec, 47.544 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036326, 30, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (379, 816, 1762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4528) [M::mem_pestat] mean and std.dev: (942.00, 753.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (194, 1568, 2672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7628) [M::mem_pestat] mean and std.dev: (1571.07, 1685.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10106) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (519, 1783, 2081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5205) [M::mem_pestat] mean and std.dev: (1364.33, 756.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6767) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1016.986 CPU sec, 44.859 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1036445, 40, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 774, 1008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2390) [M::mem_pestat] mean and std.dev: (652.50, 381.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3081) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (734, 2210, 3542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9158) [M::mem_pestat] mean and std.dev: (2507.70, 2238.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11966) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (987, 2366, 5923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15795) [M::mem_pestat] mean and std.dev: (3045.75, 2348.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20731) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1018.542 CPU sec, 50.120 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1037227, 43, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (346, 439, 2266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6106) [M::mem_pestat] mean and std.dev: (933.80, 933.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8026) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (272, 2135, 2629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7343) [M::mem_pestat] mean and std.dev: (1594.74, 1648.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9700) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1332, 1673, 4431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10629) [M::mem_pestat] mean and std.dev: (2521.18, 1974.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13728) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1016.556 CPU sec, 44.767 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 1036611, 43, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (376, 393, 1349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3295) [M::mem_pestat] mean and std.dev: (722.64, 615.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4268) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.04, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (245, 1396, 2583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7259) [M::mem_pestat] mean and std.dev: (1430.46, 1454.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9597) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1178, 1416, 1648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (238, 2588) [M::mem_pestat] mean and std.dev: (1166.11, 415.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3058) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1035.512 CPU sec, 66.779 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1036371, 34, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (376, 1245, 2131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5641) [M::mem_pestat] mean and std.dev: (1169.30, 1016.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (616, 1948, 3957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10639) [M::mem_pestat] mean and std.dev: (2823.53, 2651.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13980) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (667, 1247, 4298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11560) [M::mem_pestat] mean and std.dev: (2824.90, 2616.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1022.000 CPU sec, 47.054 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036918, 45, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (339, 415, 1270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3132) [M::mem_pestat] mean and std.dev: (637.94, 418.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4063) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (286, 1864, 3980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11368) [M::mem_pestat] mean and std.dev: (2503.42, 2643.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15062) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (526, 1239, 1953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4807) [M::mem_pestat] mean and std.dev: (1374.50, 1097.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6234) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1017.646 CPU sec, 49.273 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1036905, 28, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (388, 1562, 2212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5860) [M::mem_pestat] mean and std.dev: (1132.56, 965.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (167, 1199, 2571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7379) [M::mem_pestat] mean and std.dev: (940.87, 903.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9783) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (977, 1308, 2584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5798) [M::mem_pestat] mean and std.dev: (1510.62, 921.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7405) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1019.338 CPU sec, 45.074 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1035622, 32, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (452, 1246, 2350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6146) [M::mem_pestat] mean and std.dev: (1491.11, 1287.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8044) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (479, 1950, 4597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12833) [M::mem_pestat] mean and std.dev: (2891.03, 2967.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16951) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (866, 1304, 2072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4484) [M::mem_pestat] mean and std.dev: (1251.93, 833.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5690) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1043.763 CPU sec, 45.998 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1036828, 29, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (386, 530, 1796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4616) [M::mem_pestat] mean and std.dev: (907.48, 818.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6026) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (407, 1899, 3446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9524) [M::mem_pestat] mean and std.dev: (2409.00, 2409.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12563) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (783, 1285, 2599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6231) [M::mem_pestat] mean and std.dev: (1716.83, 1607.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8146) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1040.887 CPU sec, 51.651 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1036441, 29, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (364, 427, 1894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4954) [M::mem_pestat] mean and std.dev: (875.36, 751.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (523, 2570, 7918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22708) [M::mem_pestat] mean and std.dev: (3297.69, 3193.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 30103) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (764, 1322, 3339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8489) [M::mem_pestat] mean and std.dev: (2153.20, 1729.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11064) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1035.777 CPU sec, 45.595 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 1036854, 27, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (365, 776, 1811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4703) [M::mem_pestat] mean and std.dev: (1009.04, 861.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6149) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (245, 2331, 4122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11876) [M::mem_pestat] mean and std.dev: (2901.30, 3025.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15753) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (782, 1350, 1962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4322) [M::mem_pestat] mean and std.dev: (1249.14, 637.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5502) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1062.231 CPU sec, 71.358 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 1036304, 20, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (408, 667, 1772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4500) [M::mem_pestat] mean and std.dev: (972.89, 822.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1197, 3352, 7897) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 21297) [M::mem_pestat] mean and std.dev: (3791.20, 3148.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 27997) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (982, 1397, 2243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4765) [M::mem_pestat] mean and std.dev: (1438.00, 735.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6026) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1048.969 CPU sec, 49.060 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1035189, 29, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (408, 936, 1911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4917) [M::mem_pestat] mean and std.dev: (1058.12, 783.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (441, 1846, 5466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15516) [M::mem_pestat] mean and std.dev: (3008.14, 3078.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20541) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (522, 1058, 1439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3273) [M::mem_pestat] mean and std.dev: (932.17, 420.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4190) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1038.245 CPU sec, 52.510 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036547, 30, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (378, 460, 1504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3756) [M::mem_pestat] mean and std.dev: (686.82, 529.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4882) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (299, 2578, 3534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10004) [M::mem_pestat] mean and std.dev: (2319.63, 2243.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13239) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (614, 957, 2047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4913) [M::mem_pestat] mean and std.dev: (1074.60, 653.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6346) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1035.726 CPU sec, 45.734 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036531, 37, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (354, 492, 1428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3576) [M::mem_pestat] mean and std.dev: (883.68, 883.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (296, 1474, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7208) [M::mem_pestat] mean and std.dev: (1336.38, 1240.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (713, 1014, 1685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3629) [M::mem_pestat] mean and std.dev: (1078.69, 653.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4601) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1031.298 CPU sec, 46.506 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036105, 36, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (400, 556, 1038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2314) [M::mem_pestat] mean and std.dev: (675.19, 475.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (321, 1917, 2618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7212) [M::mem_pestat] mean and std.dev: (1676.97, 1505.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9509) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1025.618 CPU sec, 45.168 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1036738, 32, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (363, 631, 1786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4632) [M::mem_pestat] mean and std.dev: (777.79, 815.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6055) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (305, 1869, 2594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7172) [M::mem_pestat] mean and std.dev: (1575.33, 1308.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9461) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (883, 1458, 1772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3550) [M::mem_pestat] mean and std.dev: (1153.33, 526.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4439) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1053.426 CPU sec, 70.838 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036677, 35, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (288, 462, 1006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2442) [M::mem_pestat] mean and std.dev: (616.81, 524.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3160) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (594, 2564, 5767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16113) [M::mem_pestat] mean and std.dev: (3151.49, 2975.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21286) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (593, 745, 1962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4700) [M::mem_pestat] mean and std.dev: (1195.53, 896.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6069) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1039.261 CPU sec, 48.639 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1035838, 36, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (337, 420, 1471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3739) [M::mem_pestat] mean and std.dev: (734.62, 661.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (543, 2450, 2647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6855) [M::mem_pestat] mean and std.dev: (1912.28, 1535.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8959) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (739, 1188, 1892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4198) [M::mem_pestat] mean and std.dev: (1204.78, 695.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5351) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1029.089 CPU sec, 46.542 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1035709, 30, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (393, 711, 1544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3846) [M::mem_pestat] mean and std.dev: (1068.62, 847.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4997) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (288, 1116, 2875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8049) [M::mem_pestat] mean and std.dev: (1543.82, 1756.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10636) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1035.947 CPU sec, 45.656 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1036935, 39, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (394, 710, 1735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4417) [M::mem_pestat] mean and std.dev: (1037.76, 823.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (396, 1379, 2598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7002) [M::mem_pestat] mean and std.dev: (1403.38, 1145.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9204) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1002, 1370, 4182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10542) [M::mem_pestat] mean and std.dev: (2392.29, 1889.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13722) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1038.794 CPU sec, 54.486 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1037075, 32, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (706, 960, 2111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4921) [M::mem_pestat] mean and std.dev: (1252.84, 1050.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6326) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (120, 1919, 5176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15288) [M::mem_pestat] mean and std.dev: (2594.53, 2789.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20344) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (811, 1614, 3046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7516) [M::mem_pestat] mean and std.dev: (2204.81, 1873.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9751) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1040.094 CPU sec, 45.887 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1037030, 27, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (383, 539, 1049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2381) [M::mem_pestat] mean and std.dev: (682.53, 551.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3047) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (190, 1238, 2602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7426) [M::mem_pestat] mean and std.dev: (949.68, 963.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9838) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (744, 1560, 2214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5154) [M::mem_pestat] mean and std.dev: (1367.68, 741.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6624) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1049.198 CPU sec, 69.664 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 1036471, 26, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 1271, 4108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11952) [M::mem_pestat] mean and std.dev: (2119.15, 2856.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (379, 2071, 7364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 21334) [M::mem_pestat] mean and std.dev: (3435.54, 3351.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 28319) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (948, 1399, 2088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4368) [M::mem_pestat] mean and std.dev: (1341.46, 715.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5508) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1049.163 CPU sec, 49.399 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1037258, 37, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (397, 474, 1662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4192) [M::mem_pestat] mean and std.dev: (797.93, 666.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (388, 2573, 3595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10009) [M::mem_pestat] mean and std.dev: (2653.11, 2615.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13216) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (697, 1308, 2343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5635) [M::mem_pestat] mean and std.dev: (1302.75, 803.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7281) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1033.820 CPU sec, 52.893 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1036576, 32, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (357, 498, 1559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3963) [M::mem_pestat] mean and std.dev: (764.21, 729.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5165) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (184, 1319, 4155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12097) [M::mem_pestat] mean and std.dev: (2610.22, 2995.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16068) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1127, 1335, 3028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6830) [M::mem_pestat] mean and std.dev: (1911.93, 1433.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8731) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1033.277 CPU sec, 45.656 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1037083, 37, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (347, 500, 2020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5366) [M::mem_pestat] mean and std.dev: (847.16, 1036.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7039) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (780, 2565, 3757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9711) [M::mem_pestat] mean and std.dev: (2754.65, 2650.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13357) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1030.072 CPU sec, 54.781 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1035901, 37, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (395, 1027, 1899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4907) [M::mem_pestat] mean and std.dev: (913.90, 615.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (546, 2597, 8497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24399) [M::mem_pestat] mean and std.dev: (3782.59, 3325.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 32350) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1032.148 CPU sec, 45.609 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1037132, 23, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (407, 1159, 1803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4595) [M::mem_pestat] mean and std.dev: (1041.58, 958.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5991) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.12, 17.82) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (268, 1220, 3558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10138) [M::mem_pestat] mean and std.dev: (1905.74, 1844.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13428) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1069, 1435, 1934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3664) [M::mem_pestat] mean and std.dev: (1426.23, 745.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4529) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1061.932 CPU sec, 71.823 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036458, 32, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (339, 766, 1469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3729) [M::mem_pestat] mean and std.dev: (783.50, 681.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (444, 2314, 3644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10044) [M::mem_pestat] mean and std.dev: (2576.44, 2538.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13244) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (726, 2733, 6801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18951) [M::mem_pestat] mean and std.dev: (3205.17, 3080.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 25026) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1036.173 CPU sec, 48.577 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1036863, 40, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (385, 1253, 3081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8473) [M::mem_pestat] mean and std.dev: (1837.28, 1818.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11169) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.12, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (166, 2540, 3551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10321) [M::mem_pestat] mean and std.dev: (2709.05, 2856.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14137) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (533, 1282, 2044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5066) [M::mem_pestat] mean and std.dev: (1321.23, 922.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6577) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1036.348 CPU sec, 53.319 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1035643, 31, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 843, 1613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4171) [M::mem_pestat] mean and std.dev: (848.35, 726.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (344, 1881, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7112) [M::mem_pestat] mean and std.dev: (1611.00, 1523.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9368) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (646, 1164, 1794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4090) [M::mem_pestat] mean and std.dev: (1076.69, 562.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5238) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1034.202 CPU sec, 45.690 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036406, 23, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 389, 633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1351) [M::mem_pestat] mean and std.dev: (338.72, 178.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (346, 2026, 8506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24826) [M::mem_pestat] mean and std.dev: (3457.22, 3392.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 32986) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (624, 1233, 2100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5052) [M::mem_pestat] mean and std.dev: (1192.50, 758.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6528) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1030.891 CPU sec, 53.299 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1036969, 36, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (391, 398, 752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1474) [M::mem_pestat] mean and std.dev: (497.50, 283.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1835) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (381, 2079, 2970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8148) [M::mem_pestat] mean and std.dev: (1598.16, 1327.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10737) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (652, 1210, 3299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8593) [M::mem_pestat] mean and std.dev: (1900.18, 1945.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11240) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1025.438 CPU sec, 45.249 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1037196, 36, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 439, 1262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3118) [M::mem_pestat] mean and std.dev: (708.53, 600.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4046) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (282, 1851, 3605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10251) [M::mem_pestat] mean and std.dev: (2370.00, 2583.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13574) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (486, 1371, 2075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5253) [M::mem_pestat] mean and std.dev: (1503.67, 1101.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6842) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1024.049 CPU sec, 45.227 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1035778, 36, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (348, 412, 947) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2145) [M::mem_pestat] mean and std.dev: (629.48, 519.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (153, 596, 2591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7467) [M::mem_pestat] mean and std.dev: (1152.41, 1485.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9905) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1183, 1790, 2942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6460) [M::mem_pestat] mean and std.dev: (2171.06, 1536.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1058.330 CPU sec, 70.903 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1036607, 32, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (374, 423, 2950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8102) [M::mem_pestat] mean and std.dev: (1695.26, 2046.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10678) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1000, 2517, 6357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17071) [M::mem_pestat] mean and std.dev: (3355.62, 3021.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22428) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (889, 1599, 6889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18889) [M::mem_pestat] mean and std.dev: (3372.47, 3121.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 24889) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1045.035 CPU sec, 49.143 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1037121, 32, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (428, 1040, 1667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4145) [M::mem_pestat] mean and std.dev: (1118.10, 782.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (496, 2557, 2900) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7708) [M::mem_pestat] mean and std.dev: (2161.77, 1862.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10112) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (421, 997, 1426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3436) [M::mem_pestat] mean and std.dev: (887.88, 587.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4441) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1033.869 CPU sec, 53.064 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 1036401, 40, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (364, 641, 2173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5791) [M::mem_pestat] mean and std.dev: (1148.70, 1177.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (515, 1972, 2981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7913) [M::mem_pestat] mean and std.dev: (1674.40, 1359.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10379) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (574, 1040, 1969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4759) [M::mem_pestat] mean and std.dev: (901.79, 478.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6154) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1031.632 CPU sec, 45.525 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 1036692, 26, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (435, 1335, 2283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5979) [M::mem_pestat] mean and std.dev: (1415.96, 1269.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (97, 1095, 2594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7588) [M::mem_pestat] mean and std.dev: (1387.33, 1718.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10085) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (719, 1529, 3079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7799) [M::mem_pestat] mean and std.dev: (1790.38, 1492.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10159) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1032.865 CPU sec, 53.923 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1036189, 35, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (386, 431, 2353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6287) [M::mem_pestat] mean and std.dev: (1481.11, 1541.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8254) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (782, 1873, 2651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6389) [M::mem_pestat] mean and std.dev: (1600.13, 1226.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8258) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (377, 1255, 1733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4445) [M::mem_pestat] mean and std.dev: (1026.75, 750.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5801) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1028.412 CPU sec, 45.413 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036678, 36, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (368, 1048, 2125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5639) [M::mem_pestat] mean and std.dev: (1342.21, 1487.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (208, 1648, 2601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7387) [M::mem_pestat] mean and std.dev: (1203.60, 1166.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9780) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (735, 1216, 2652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6486) [M::mem_pestat] mean and std.dev: (1838.53, 1657.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8469) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1054.676 CPU sec, 69.548 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 1036944, 26, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (360, 749, 1834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4782) [M::mem_pestat] mean and std.dev: (1028.22, 867.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6256) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (268, 2062, 6331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18457) [M::mem_pestat] mean and std.dev: (3278.96, 3213.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 24520) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1032.994 CPU sec, 48.675 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036191, 29, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (379, 808, 1494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3724) [M::mem_pestat] mean and std.dev: (1047.95, 926.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4839) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (363, 2269, 3985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11229) [M::mem_pestat] mean and std.dev: (2943.86, 2899.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14851) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (418, 808, 2496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6652) [M::mem_pestat] mean and std.dev: (1727.00, 1939.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9485) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1044.728 CPU sec, 52.223 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1036423, 36, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (610, 1350, 4443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12109) [M::mem_pestat] mean and std.dev: (2715.44, 3370.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16199) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (555, 2524, 3992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10866) [M::mem_pestat] mean and std.dev: (2930.17, 3009.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14967) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1030.353 CPU sec, 45.525 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1036141, 24, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (308, 1054, 1967) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5285) [M::mem_pestat] mean and std.dev: (1033.57, 1004.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1478, 2583, 5919) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14801) [M::mem_pestat] mean and std.dev: (3399.96, 2858.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19242) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1039.424 CPU sec, 53.458 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1036229, 34, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (342, 486, 2585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7071) [M::mem_pestat] mean and std.dev: (1341.90, 1490.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (727, 2572, 2668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6550) [M::mem_pestat] mean and std.dev: (1978.31, 1350.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8491) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (809, 1599, 2142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4808) [M::mem_pestat] mean and std.dev: (1448.45, 1133.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6141) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1028.625 CPU sec, 45.426 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1037324, 26, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (414, 664, 1201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2775) [M::mem_pestat] mean and std.dev: (721.07, 577.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (388, 1886, 4154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11686) [M::mem_pestat] mean and std.dev: (2700.81, 2669.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15452) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1132, 1921, 5062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12922) [M::mem_pestat] mean and std.dev: (2700.24, 2175.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16852) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1047.790 CPU sec, 69.179 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 1036379, 33, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (363, 511, 1748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4518) [M::mem_pestat] mean and std.dev: (1083.41, 1087.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5903) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (493, 2488, 3568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9718) [M::mem_pestat] mean and std.dev: (2790.15, 2710.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13634) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (894, 1174, 1805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3627) [M::mem_pestat] mean and std.dev: (1162.91, 592.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4538) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1029.860 CPU sec, 48.321 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 1036409, 24, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 411, 1235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3071) [M::mem_pestat] mean and std.dev: (598.75, 484.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (141, 1871, 2592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7494) [M::mem_pestat] mean and std.dev: (1601.83, 1588.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9945) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (881, 2442, 3898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9932) [M::mem_pestat] mean and std.dev: (2986.50, 2581.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13313) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1036.836 CPU sec, 53.907 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1035968, 39, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (839, 1170, 3128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7706) [M::mem_pestat] mean and std.dev: (2343.71, 2122.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.05, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (580, 2314, 2951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7693) [M::mem_pestat] mean and std.dev: (2146.24, 1779.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10064) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1003, 1471, 2621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5857) [M::mem_pestat] mean and std.dev: (1778.91, 1139.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7475) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1026.859 CPU sec, 45.419 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1037146, 40, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (403, 681, 3049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8341) [M::mem_pestat] mean and std.dev: (1789.76, 2022.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10987) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (553, 1926, 6309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17821) [M::mem_pestat] mean and std.dev: (3116.30, 3048.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23577) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1275, 1740, 3235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7155) [M::mem_pestat] mean and std.dev: (1891.62, 1200.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9115) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1029.635 CPU sec, 54.282 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1035900, 28, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (470, 1225, 5843) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16589) [M::mem_pestat] mean and std.dev: (2670.47, 3010.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21962) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (320, 2065, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7178) [M::mem_pestat] mean and std.dev: (1811.08, 1619.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9464) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1642, 2963, 4887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11377) [M::mem_pestat] mean and std.dev: (3089.13, 2207.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14622) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1026.971 CPU sec, 45.258 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1036413, 28, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (758, 1250, 2123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4853) [M::mem_pestat] mean and std.dev: (1282.05, 938.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6218) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (416, 2154, 3293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9047) [M::mem_pestat] mean and std.dev: (2587.86, 2681.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13312) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (436, 814, 1868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4732) [M::mem_pestat] mean and std.dev: (1094.91, 858.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6164) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1059.208 CPU sec, 69.462 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036314, 43, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (399, 1095, 2913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7941) [M::mem_pestat] mean and std.dev: (1928.20, 2110.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1489, 2585, 5369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13129) [M::mem_pestat] mean and std.dev: (3467.16, 2803.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17009) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1280, 1600, 4292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10316) [M::mem_pestat] mean and std.dev: (2519.92, 1921.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13328) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1044.419 CPU sec, 48.671 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1035747, 25, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 920, 3501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9823) [M::mem_pestat] mean and std.dev: (1962.85, 2423.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12984) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (345, 2401, 6756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19578) [M::mem_pestat] mean and std.dev: (3128.92, 3103.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 25989) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1351, 1728, 3949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9145) [M::mem_pestat] mean and std.dev: (2506.47, 2060.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11743) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1037.463 CPU sec, 53.824 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036765, 30, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (375, 592, 1823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4719) [M::mem_pestat] mean and std.dev: (950.86, 960.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6167) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.12, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (292, 2522, 2978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8350) [M::mem_pestat] mean and std.dev: (1967.89, 1765.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11036) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (674, 1112, 2631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6545) [M::mem_pestat] mean and std.dev: (1258.56, 847.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8502) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1029.013 CPU sec, 45.498 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1036423, 32, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (385, 537, 1075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2455) [M::mem_pestat] mean and std.dev: (659.27, 381.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3145) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (199, 626, 2594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7384) [M::mem_pestat] mean and std.dev: (1443.90, 1723.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9779) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1026, 1463, 2093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4227) [M::mem_pestat] mean and std.dev: (1461.14, 777.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5294) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1035.717 CPU sec, 48.942 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1035853, 42, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 434, 1409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3629) [M::mem_pestat] mean and std.dev: (583.50, 472.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (523, 1904, 3045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8089) [M::mem_pestat] mean and std.dev: (1974.70, 1601.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10611) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1071, 1626, 4679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11895) [M::mem_pestat] mean and std.dev: (2732.52, 2510.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15503) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1033.458 CPU sec, 45.549 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1036350, 31, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (307, 958, 1465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3781) [M::mem_pestat] mean and std.dev: (852.62, 638.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4939) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1399, 2574, 5346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13240) [M::mem_pestat] mean and std.dev: (3482.16, 3030.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17187) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1018.112 CPU sec, 44.936 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 1036994, 31, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (381, 637, 1060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2418) [M::mem_pestat] mean and std.dev: (634.26, 335.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3097) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1959, 2832, 8265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 20877) [M::mem_pestat] mean and std.dev: (4140.48, 3101.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 27183) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1011, 1545, 4532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11574) [M::mem_pestat] mean and std.dev: (2251.31, 1737.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1050.004 CPU sec, 69.027 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1035604, 33, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (401, 491, 1291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3071) [M::mem_pestat] mean and std.dev: (625.00, 474.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (196, 737, 2894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8290) [M::mem_pestat] mean and std.dev: (1370.90, 2000.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10988) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (814, 1327, 2219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5029) [M::mem_pestat] mean and std.dev: (1206.70, 704.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6434) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1038.658 CPU sec, 48.763 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 1036736, 25, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (724, 1084, 4148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10996) [M::mem_pestat] mean and std.dev: (2405.57, 2550.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (357, 2174, 3949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11133) [M::mem_pestat] mean and std.dev: (2696.64, 2924.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14725) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1126, 1301, 1822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3214) [M::mem_pestat] mean and std.dev: (1237.08, 645.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3910) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1022.768 CPU sec, 49.557 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 1036501, 30, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (385, 562, 1252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2986) [M::mem_pestat] mean and std.dev: (807.17, 635.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (398, 2591, 5120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14564) [M::mem_pestat] mean and std.dev: (3101.10, 2809.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19286) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (477, 1201, 2194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5628) [M::mem_pestat] mean and std.dev: (1169.48, 884.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7345) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1030.555 CPU sec, 45.536 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 1036611, 33, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (405, 844, 3624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10062) [M::mem_pestat] mean and std.dev: (1897.71, 2098.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13281) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (608, 2591, 7132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 20180) [M::mem_pestat] mean and std.dev: (3347.03, 3025.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 26704) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (680, 821, 3005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7655) [M::mem_pestat] mean and std.dev: (1648.27, 1552.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9980) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1032.735 CPU sec, 51.159 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036460, 35, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (354, 789, 2333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6291) [M::mem_pestat] mean and std.dev: (1233.38, 1192.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8270) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (248, 1748, 2929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8291) [M::mem_pestat] mean and std.dev: (1728.34, 1681.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10972) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (779, 1614, 3471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8855) [M::mem_pestat] mean and std.dev: (2020.71, 1428.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11547) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1024.623 CPU sec, 45.117 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1036425, 37, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (369, 616, 1468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3666) [M::mem_pestat] mean and std.dev: (884.82, 860.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (388, 2056, 4295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12109) [M::mem_pestat] mean and std.dev: (2911.05, 2947.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16016) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1050.531 CPU sec, 69.182 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1036104, 44, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (372, 804, 3041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8379) [M::mem_pestat] mean and std.dev: (1705.40, 1875.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11048) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (357, 1567, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7086) [M::mem_pestat] mean and std.dev: (1455.62, 1342.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9329) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (857, 1237, 2305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5201) [M::mem_pestat] mean and std.dev: (1597.35, 1219.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6649) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1029.459 CPU sec, 48.346 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036202, 35, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (393, 1220, 1957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5085) [M::mem_pestat] mean and std.dev: (1477.95, 1283.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (304, 2441, 6837) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19903) [M::mem_pestat] mean and std.dev: (3184.69, 3158.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 26436) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (887, 1103, 2706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6344) [M::mem_pestat] mean and std.dev: (1341.29, 1087.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8163) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1024.880 CPU sec, 47.731 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1036347, 28, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (368, 402, 1443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3593) [M::mem_pestat] mean and std.dev: (722.43, 712.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (544, 2105, 3531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9505) [M::mem_pestat] mean and std.dev: (2577.75, 2642.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13149) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1036, 1380, 5054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13090) [M::mem_pestat] mean and std.dev: (2841.38, 2846.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17108) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1018.167 CPU sec, 44.973 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1036225, 24, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (552, 832, 1461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3279) [M::mem_pestat] mean and std.dev: (1006.13, 589.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4188) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.12, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (265, 1440, 3438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9784) [M::mem_pestat] mean and std.dev: (2531.29, 2826.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13836) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (943, 1440, 2357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5185) [M::mem_pestat] mean and std.dev: (1404.94, 876.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6599) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1020.094 CPU sec, 47.620 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036155, 31, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (401, 997, 1804) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4610) [M::mem_pestat] mean and std.dev: (973.36, 735.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6013) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1105, 2579, 2908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6514) [M::mem_pestat] mean and std.dev: (1902.69, 1441.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8317) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (512, 1210, 1778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4310) [M::mem_pestat] mean and std.dev: (987.79, 536.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5576) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1013.811 CPU sec, 44.664 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1037191, 24, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (505, 1364, 2761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7273) [M::mem_pestat] mean and std.dev: (1746.18, 1368.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (131, 2590, 4657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13709) [M::mem_pestat] mean and std.dev: (2898.79, 3253.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18235) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1252, 1636, 2405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4711) [M::mem_pestat] mean and std.dev: (1749.46, 684.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5864) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1035.592 CPU sec, 69.108 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1036457, 31, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (382, 913, 2860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7816) [M::mem_pestat] mean and std.dev: (1698.04, 1940.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10294) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (411, 1430, 2597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6969) [M::mem_pestat] mean and std.dev: (1667.77, 1472.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9155) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (555, 1032, 1842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4416) [M::mem_pestat] mean and std.dev: (1313.87, 1048.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5703) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1020.471 CPU sec, 47.755 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036009, 34, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (379, 764, 1684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4294) [M::mem_pestat] mean and std.dev: (972.05, 886.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (312, 2106, 2601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7179) [M::mem_pestat] mean and std.dev: (1763.22, 1371.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9468) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (859, 1058, 1859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3859) [M::mem_pestat] mean and std.dev: (1212.41, 795.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4859) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1023.714 CPU sec, 48.119 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1036544, 25, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (354, 605, 1400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3492) [M::mem_pestat] mean and std.dev: (737.24, 637.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (191, 2032, 2924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8390) [M::mem_pestat] mean and std.dev: (1663.95, 1759.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11123) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1228, 1665, 5278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13378) [M::mem_pestat] mean and std.dev: (2890.58, 2589.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17428) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1020.593 CPU sec, 44.985 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1037140, 35, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (460, 1020, 1699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4177) [M::mem_pestat] mean and std.dev: (788.67, 508.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (158, 2277, 3090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8954) [M::mem_pestat] mean and std.dev: (2168.46, 2234.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11886) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (953, 1004, 1685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3149) [M::mem_pestat] mean and std.dev: (1295.31, 588.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3881) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1012.598 CPU sec, 48.416 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036800, 23, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (369, 1133, 1439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3579) [M::mem_pestat] mean and std.dev: (889.53, 570.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (511, 2507, 4170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11488) [M::mem_pestat] mean and std.dev: (2731.30, 2501.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15147) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1258, 1645, 4243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10213) [M::mem_pestat] mean and std.dev: (2534.83, 2107.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13198) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1012.172 CPU sec, 44.591 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1036268, 40, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (390, 892, 4595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13005) [M::mem_pestat] mean and std.dev: (2199.29, 2369.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17210) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (164, 1441, 3565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10367) [M::mem_pestat] mean and std.dev: (2300.88, 2610.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13768) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (818, 1414, 1608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3188) [M::mem_pestat] mean and std.dev: (1040.00, 486.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3978) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1041.746 CPU sec, 69.248 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1035376, 35, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (362, 585, 1835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4781) [M::mem_pestat] mean and std.dev: (902.20, 797.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6254) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (290, 1356, 3407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9641) [M::mem_pestat] mean and std.dev: (2469.66, 2728.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13382) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1124, 2872, 3867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9353) [M::mem_pestat] mean and std.dev: (2564.71, 1595.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12096) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1019.987 CPU sec, 47.507 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 1036322, 34, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (393, 784, 1512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3750) [M::mem_pestat] mean and std.dev: (867.52, 632.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (802, 2272, 4176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10924) [M::mem_pestat] mean and std.dev: (3162.03, 2778.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14298) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (405, 1060, 1715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4335) [M::mem_pestat] mean and std.dev: (1137.65, 927.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5645) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1017.578 CPU sec, 50.028 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1035417, 36, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (405, 656, 3288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9054) [M::mem_pestat] mean and std.dev: (1940.08, 2406.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11937) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (783, 2546, 3593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9213) [M::mem_pestat] mean and std.dev: (2542.39, 2257.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12023) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (438, 1370, 6320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18084) [M::mem_pestat] mean and std.dev: (2870.65, 2811.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23966) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1015.335 CPU sec, 44.977 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036146, 40, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 364, 2014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5572) [M::mem_pestat] mean and std.dev: (956.88, 1392.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.12, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (371, 1443, 4811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13691) [M::mem_pestat] mean and std.dev: (2872.72, 3093.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18131) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (842, 1387, 2736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6524) [M::mem_pestat] mean and std.dev: (1652.00, 1159.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8418) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1013.595 CPU sec, 49.594 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 1036452, 35, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (402, 731, 2092) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5472) [M::mem_pestat] mean and std.dev: (1102.86, 925.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7162) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1450, 2580, 3722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8266) [M::mem_pestat] mean and std.dev: (2470.19, 1734.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10538) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (959, 1513, 2756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6350) [M::mem_pestat] mean and std.dev: (1454.67, 801.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8147) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1018.234 CPU sec, 44.928 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1036246, 32, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (383, 791, 2294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6116) [M::mem_pestat] mean and std.dev: (1160.29, 1264.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8027) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (280, 1351, 3850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10990) [M::mem_pestat] mean and std.dev: (2366.38, 2750.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14560) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1252, 2849, 3574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8218) [M::mem_pestat] mean and std.dev: (2671.86, 1456.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10540) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1014.340 CPU sec, 44.846 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1035692, 35, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (499, 923, 2744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7234) [M::mem_pestat] mean and std.dev: (1774.82, 1921.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (167, 1377, 3799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11063) [M::mem_pestat] mean and std.dev: (2545.57, 2945.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14695) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (697, 926, 1661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3589) [M::mem_pestat] mean and std.dev: (1137.67, 632.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4553) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1030.379 CPU sec, 68.362 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036838, 25, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (523, 1525, 3662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9940) [M::mem_pestat] mean and std.dev: (2014.35, 2179.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13079) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (183, 1652, 8478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25068) [M::mem_pestat] mean and std.dev: (2930.60, 3288.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 33363) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (445, 1313, 1472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3526) [M::mem_pestat] mean and std.dev: (1025.27, 596.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4553) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1016.800 CPU sec, 47.524 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1036704, 37, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (385, 750, 1396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3418) [M::mem_pestat] mean and std.dev: (912.21, 899.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.12, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (686, 2577, 3986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10586) [M::mem_pestat] mean and std.dev: (2736.22, 2077.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13886) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (361, 707, 1654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4240) [M::mem_pestat] mean and std.dev: (831.50, 563.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5533) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1019.630 CPU sec, 49.544 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036670, 38, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (333, 422, 1695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4419) [M::mem_pestat] mean and std.dev: (638.87, 561.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.82) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (356, 1883, 3838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10802) [M::mem_pestat] mean and std.dev: (2753.47, 2931.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14479) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (687, 930, 1234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2328) [M::mem_pestat] mean and std.dev: (902.47, 491.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2875) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1018.355 CPU sec, 44.956 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1037069, 28, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (352, 727, 2410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6526) [M::mem_pestat] mean and std.dev: (1141.75, 1198.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (283, 1858, 2571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7147) [M::mem_pestat] mean and std.dev: (1673.77, 1596.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9435) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (471, 2471, 4522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12624) [M::mem_pestat] mean and std.dev: (2313.36, 2059.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16675) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1006.612 CPU sec, 45.909 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1036026, 34, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (357, 1064, 2932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8082) [M::mem_pestat] mean and std.dev: (1660.69, 1652.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (287, 1873, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7226) [M::mem_pestat] mean and std.dev: (1370.07, 1337.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9539) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1035, 1752, 7173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19449) [M::mem_pestat] mean and std.dev: (3695.40, 3280.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 25587) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1013.321 CPU sec, 44.609 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036357, 34, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (326, 641, 1526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3926) [M::mem_pestat] mean and std.dev: (684.12, 505.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5126) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (187, 2085, 4700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13726) [M::mem_pestat] mean and std.dev: (2771.26, 3066.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18239) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1076, 1339, 3342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7874) [M::mem_pestat] mean and std.dev: (1842.00, 1628.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10140) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1026.446 CPU sec, 67.060 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036066, 37, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (379, 794, 1373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3361) [M::mem_pestat] mean and std.dev: (750.94, 521.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (117, 1316, 2561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7449) [M::mem_pestat] mean and std.dev: (1432.80, 1541.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9893) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1132, 1671, 3903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9445) [M::mem_pestat] mean and std.dev: (2219.10, 1873.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12216) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1021.319 CPU sec, 47.683 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1037857, 28, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (357, 840, 1567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3987) [M::mem_pestat] mean and std.dev: (896.24, 735.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5197) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1093, 2593, 4091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10087) [M::mem_pestat] mean and std.dev: (3033.36, 2685.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13776) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (552, 1093, 1995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4881) [M::mem_pestat] mean and std.dev: (1265.24, 990.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6324) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1015.892 CPU sec, 50.121 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1036201, 29, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (589, 1737, 3736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10030) [M::mem_pestat] mean and std.dev: (2280.61, 2028.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13177) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (838, 1859, 3583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9073) [M::mem_pestat] mean and std.dev: (2610.76, 2440.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12373) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (911, 1560, 3806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9596) [M::mem_pestat] mean and std.dev: (2225.43, 1941.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12491) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1010.805 CPU sec, 44.541 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1036256, 25, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (403, 825, 1349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3241) [M::mem_pestat] mean and std.dev: (783.67, 539.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4187) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (992, 2608, 5450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14366) [M::mem_pestat] mean and std.dev: (3352.28, 2952.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18824) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (571, 1417, 2733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7057) [M::mem_pestat] mean and std.dev: (2055.25, 1855.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9479) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1010.140 CPU sec, 46.803 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1036843, 32, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (406, 1194, 2958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8062) [M::mem_pestat] mean and std.dev: (1988.44, 2126.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (314, 1467, 2592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7148) [M::mem_pestat] mean and std.dev: (1412.71, 1301.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9426) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (858, 1313, 1578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3018) [M::mem_pestat] mean and std.dev: (1268.00, 782.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4398) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1013.999 CPU sec, 44.655 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036606, 41, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (389, 903, 1842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4748) [M::mem_pestat] mean and std.dev: (1370.32, 1285.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (168, 1936, 7359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 21741) [M::mem_pestat] mean and std.dev: (3032.59, 3351.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 28932) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (788, 2663, 3650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9374) [M::mem_pestat] mean and std.dev: (2759.72, 2508.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12793) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1033.748 CPU sec, 68.245 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1035811, 39, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (353, 453, 1085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2549) [M::mem_pestat] mean and std.dev: (699.88, 552.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3281) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1124, 1890, 2652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5708) [M::mem_pestat] mean and std.dev: (1755.40, 1221.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7236) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1017.939 CPU sec, 47.145 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1036879, 33, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (457, 991, 1593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3865) [M::mem_pestat] mean and std.dev: (981.11, 576.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5001) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (503, 1871, 2608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6818) [M::mem_pestat] mean and std.dev: (1629.57, 1425.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8923) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (838, 1105, 1463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2713) [M::mem_pestat] mean and std.dev: (916.18, 321.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3338) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1009.406 CPU sec, 46.108 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036613, 34, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (275, 472, 1187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3011) [M::mem_pestat] mean and std.dev: (687.58, 659.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3923) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (889, 2585, 3779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9559) [M::mem_pestat] mean and std.dev: (2982.12, 2673.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13676) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1012.854 CPU sec, 44.597 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1036482, 35, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (352, 859, 1871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4909) [M::mem_pestat] mean and std.dev: (895.43, 678.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.05, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (236, 1354, 3426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9806) [M::mem_pestat] mean and std.dev: (2299.94, 2646.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12996) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (561, 1364, 1991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4851) [M::mem_pestat] mean and std.dev: (1346.71, 860.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6281) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1009.757 CPU sec, 46.908 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036776, 22, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (380, 726, 2429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6527) [M::mem_pestat] mean and std.dev: (1263.05, 1384.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1384, 1897, 2848) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5776) [M::mem_pestat] mean and std.dev: (1762.16, 1145.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7240) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (993, 1474, 4390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11184) [M::mem_pestat] mean and std.dev: (2340.59, 1894.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14581) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1015.366 CPU sec, 44.701 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 1036651, 36, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (225, 1619, 2703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7659) [M::mem_pestat] mean and std.dev: (1640.42, 1780.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10137) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (774, 1304, 2062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4638) [M::mem_pestat] mean and std.dev: (1242.42, 668.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5926) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1045.281 CPU sec, 70.391 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1037125, 30, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (386, 575, 1405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3443) [M::mem_pestat] mean and std.dev: (609.13, 380.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (120, 1855, 2590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7530) [M::mem_pestat] mean and std.dev: (1384.74, 1592.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10000) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1024.330 CPU sec, 47.458 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1036519, 40, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (347, 423, 1116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2654) [M::mem_pestat] mean and std.dev: (592.32, 391.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.13, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (389, 1382, 3310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9152) [M::mem_pestat] mean and std.dev: (2252.18, 2422.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12073) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (397, 1179, 1836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4714) [M::mem_pestat] mean and std.dev: (920.67, 574.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6153) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1017.056 CPU sec, 50.585 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1036808, 23, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (363, 870, 3720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10434) [M::mem_pestat] mean and std.dev: (1890.75, 2201.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.12, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (162, 787, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7491) [M::mem_pestat] mean and std.dev: (1276.81, 1509.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9934) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (844, 1153, 2159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4789) [M::mem_pestat] mean and std.dev: (1298.27, 812.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6104) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1014.948 CPU sec, 44.728 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 1036387, 32, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (379, 728, 1819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4699) [M::mem_pestat] mean and std.dev: (856.77, 633.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6139) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (373, 1703, 3444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9586) [M::mem_pestat] mean and std.dev: (2367.03, 2639.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12924) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1005, 1669, 2564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5682) [M::mem_pestat] mean and std.dev: (2039.71, 1421.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7724) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1016.189 CPU sec, 50.830 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036844, 30, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (320, 967, 2177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5891) [M::mem_pestat] mean and std.dev: (1012.15, 998.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (352, 2483, 3962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11182) [M::mem_pestat] mean and std.dev: (2807.53, 2605.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14792) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1012.551 CPU sec, 44.650 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1036137, 35, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 424, 1031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2459) [M::mem_pestat] mean and std.dev: (684.84, 663.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (515, 1936, 4377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12101) [M::mem_pestat] mean and std.dev: (2931.74, 2966.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15963) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (737, 1433, 2368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5630) [M::mem_pestat] mean and std.dev: (1496.33, 1182.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7261) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1008.678 CPU sec, 44.523 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1036284, 33, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (353, 489, 2029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5381) [M::mem_pestat] mean and std.dev: (1103.74, 1240.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7057) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1067, 1993, 3576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8594) [M::mem_pestat] mean and std.dev: (2578.39, 2136.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11124) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1060.055 CPU sec, 70.000 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 1036539, 27, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (397, 873, 1472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3622) [M::mem_pestat] mean and std.dev: (1034.00, 894.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (400, 1059, 2568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6904) [M::mem_pestat] mean and std.dev: (1146.88, 961.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9072) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (969, 1400, 1981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4005) [M::mem_pestat] mean and std.dev: (1507.70, 1049.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5706) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1034.584 CPU sec, 48.672 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1036092, 35, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (370, 590, 2126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5638) [M::mem_pestat] mean and std.dev: (1110.44, 1004.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (187, 1878, 8318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24580) [M::mem_pestat] mean and std.dev: (3370.86, 3436.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 32711) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (927, 1315, 3542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8772) [M::mem_pestat] mean and std.dev: (2012.23, 1526.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11387) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1024.899 CPU sec, 52.817 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036239, 25, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (365, 465, 1359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3347) [M::mem_pestat] mean and std.dev: (675.47, 544.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4341) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.05, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1183, 2472, 3427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7915) [M::mem_pestat] mean and std.dev: (2163.17, 1800.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10159) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1258, 1999, 3390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7654) [M::mem_pestat] mean and std.dev: (2483.83, 1960.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10327) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1021.637 CPU sec, 45.081 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1037206, 32, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (341, 762, 1525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3893) [M::mem_pestat] mean and std.dev: (922.72, 969.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5077) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (297, 1731, 2945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8241) [M::mem_pestat] mean and std.dev: (1683.34, 1641.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10889) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (428, 1270, 1911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4877) [M::mem_pestat] mean and std.dev: (1190.88, 710.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6360) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1028.205 CPU sec, 53.769 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 1036874, 26, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (360, 625, 1336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3288) [M::mem_pestat] mean and std.dev: (671.77, 446.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4264) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (152, 1464, 3592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10472) [M::mem_pestat] mean and std.dev: (2435.15, 2705.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13912) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (874, 1120, 1524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2824) [M::mem_pestat] mean and std.dev: (928.90, 415.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3474) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1023.904 CPU sec, 45.291 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036492, 27, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (349, 418, 664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1294) [M::mem_pestat] mean and std.dev: (434.89, 214.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (183, 1873, 2587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7395) [M::mem_pestat] mean and std.dev: (1692.92, 1580.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9799) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (706, 1586, 4288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11452) [M::mem_pestat] mean and std.dev: (2633.28, 2634.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15034) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1057.970 CPU sec, 69.467 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 1036063, 43, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (363, 643, 1376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3402) [M::mem_pestat] mean and std.dev: (723.71, 506.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (266, 1274, 3975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11393) [M::mem_pestat] mean and std.dev: (2247.07, 2431.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15102) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1014, 1557, 3812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9408) [M::mem_pestat] mean and std.dev: (2092.94, 1896.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12206) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1028.129 CPU sec, 48.156 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1036548, 38, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (382, 684, 1508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3760) [M::mem_pestat] mean and std.dev: (976.44, 939.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4886) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (464, 1912, 5334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15074) [M::mem_pestat] mean and std.dev: (2872.11, 2877.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19944) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (811, 1433, 6568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18082) [M::mem_pestat] mean and std.dev: (2863.29, 2876.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23839) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1027.451 CPU sec, 51.306 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 1036474, 26, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (402, 973, 2589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6963) [M::mem_pestat] mean and std.dev: (1346.38, 1237.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9150) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1105, 2611, 3965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9685) [M::mem_pestat] mean and std.dev: (2874.35, 2227.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12545) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (730, 1240, 2570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6250) [M::mem_pestat] mean and std.dev: (1480.58, 1299.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8090) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1019.274 CPU sec, 45.045 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036252, 30, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (337, 720, 2092) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5602) [M::mem_pestat] mean and std.dev: (1131.42, 1217.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.13, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (531, 1993, 3617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9789) [M::mem_pestat] mean and std.dev: (2536.70, 2467.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12875) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (626, 1433, 2299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5645) [M::mem_pestat] mean and std.dev: (1504.67, 982.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7318) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1021.143 CPU sec, 49.408 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036555, 28, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (365, 454, 1171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2783) [M::mem_pestat] mean and std.dev: (632.89, 469.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (2146, 3634, 5259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11485) [M::mem_pestat] mean and std.dev: (3825.43, 2516.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14598) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1855, 2498, 5991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14263) [M::mem_pestat] mean and std.dev: (3578.00, 2534.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18399) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1017.105 CPU sec, 44.828 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036000, 41, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (398, 1181, 2211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5837) [M::mem_pestat] mean and std.dev: (1173.47, 891.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (376, 2459, 4618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13102) [M::mem_pestat] mean and std.dev: (2928.78, 2925.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17344) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (686, 1031, 2576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6356) [M::mem_pestat] mean and std.dev: (1451.41, 1169.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8246) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1044.731 CPU sec, 67.512 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1035172, 44, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (333, 677, 2617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7185) [M::mem_pestat] mean and std.dev: (1714.24, 1808.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (226, 1489, 3608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10372) [M::mem_pestat] mean and std.dev: (2480.36, 2672.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13754) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (406, 1323, 2592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6964) [M::mem_pestat] mean and std.dev: (1896.53, 1771.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9150) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1023.275 CPU sec, 47.568 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1035928, 32, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 501, 2826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7818) [M::mem_pestat] mean and std.dev: (1330.05, 1529.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (257, 1832, 7359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 21563) [M::mem_pestat] mean and std.dev: (2936.34, 3258.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 28665) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1189, 1441, 5529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14209) [M::mem_pestat] mean and std.dev: (2778.05, 2402.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18549) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1018.763 CPU sec, 48.853 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1036063, 30, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (390, 660, 2004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5232) [M::mem_pestat] mean and std.dev: (1076.00, 1097.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (140, 2154, 5217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15371) [M::mem_pestat] mean and std.dev: (2893.20, 3052.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20448) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1004, 1976, 3098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7286) [M::mem_pestat] mean and std.dev: (1795.70, 1078.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9380) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1021.484 CPU sec, 45.051 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1035594, 33, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (284, 436, 1158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2906) [M::mem_pestat] mean and std.dev: (626.56, 534.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1351, 2597, 7889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 20965) [M::mem_pestat] mean and std.dev: (3666.76, 3166.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 27503) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (735, 1393, 1954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4392) [M::mem_pestat] mean and std.dev: (1263.95, 832.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5611) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1014.985 CPU sec, 48.237 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 1037164, 27, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (523, 1310, 1638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3868) [M::mem_pestat] mean and std.dev: (1061.08, 610.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (224, 346, 2594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7334) [M::mem_pestat] mean and std.dev: (1101.72, 1340.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9704) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (676, 1161, 3958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10522) [M::mem_pestat] mean and std.dev: (2659.21, 2731.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13804) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1019.372 CPU sec, 44.961 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1036804, 27, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (288, 407, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972) [M::mem_pestat] mean and std.dev: (314.00, 152.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1200) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.88) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (399, 2073, 3982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11148) [M::mem_pestat] mean and std.dev: (2547.85, 2457.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14731) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (415, 1062, 1738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4384) [M::mem_pestat] mean and std.dev: (1013.53, 977.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5707) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1037.820 CPU sec, 69.495 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1036804, 40, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (343, 822, 2028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5398) [M::mem_pestat] mean and std.dev: (1107.25, 1076.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7083) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (91, 1962, 5253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15577) [M::mem_pestat] mean and std.dev: (2803.53, 2980.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20739) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1291, 1827, 4552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11074) [M::mem_pestat] mean and std.dev: (2700.50, 2140.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14335) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1020.931 CPU sec, 47.644 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1037177, 31, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (280, 636, 1806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4858) [M::mem_pestat] mean and std.dev: (746.44, 781.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1160, 2597, 3974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9602) [M::mem_pestat] mean and std.dev: (3031.13, 2787.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14182) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (450, 1073, 2753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7359) [M::mem_pestat] mean and std.dev: (1176.08, 936.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9662) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1018.945 CPU sec, 50.656 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 1036731, 19, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (783, 1011, 1767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3735) [M::mem_pestat] mean and std.dev: (1048.96, 542.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (233, 708, 2567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7235) [M::mem_pestat] mean and std.dev: (1167.83, 1078.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9569) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1119, 1673, 2155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4227) [M::mem_pestat] mean and std.dev: (1586.00, 715.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5263) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1012.436 CPU sec, 44.690 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1036013, 27, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (374, 437, 1347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3293) [M::mem_pestat] mean and std.dev: (771.28, 656.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4266) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (282, 1549, 5239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15153) [M::mem_pestat] mean and std.dev: (2763.70, 2964.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20110) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1055, 1213, 3463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8279) [M::mem_pestat] mean and std.dev: (2253.93, 2092.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10687) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1015.853 CPU sec, 45.689 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1036667, 31, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 376, 1276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3152) [M::mem_pestat] mean and std.dev: (555.60, 456.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4090) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (256, 398, 2331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6481) [M::mem_pestat] mean and std.dev: (1296.23, 1585.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8556) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (925, 1216, 1686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3208) [M::mem_pestat] mean and std.dev: (1145.20, 601.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3969) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1019.519 CPU sec, 44.910 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1037215, 29, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (374, 971, 2157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5723) [M::mem_pestat] mean and std.dev: (908.11, 759.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (217, 2574, 4814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14008) [M::mem_pestat] mean and std.dev: (2996.00, 2936.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18605) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (473, 1233, 2195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5639) [M::mem_pestat] mean and std.dev: (1195.92, 978.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7361) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1017.621 CPU sec, 45.122 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1035986, 17, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (546, 767, 1662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3894) [M::mem_pestat] mean and std.dev: (1116.12, 849.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5010) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (345, 1464, 3328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9294) [M::mem_pestat] mean and std.dev: (2013.76, 2063.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12277) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (818, 1249, 2802) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6770) [M::mem_pestat] mean and std.dev: (1927.38, 1966.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9791) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1042.600 CPU sec, 68.245 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 1036306, 27, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (373, 401, 1187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2815) [M::mem_pestat] mean and std.dev: (621.75, 461.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (327, 1848, 4730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13536) [M::mem_pestat] mean and std.dev: (2747.81, 2896.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17939) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (661, 1366, 2657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6649) [M::mem_pestat] mean and std.dev: (1894.05, 1663.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8645) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1026.645 CPU sec, 48.118 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036182, 42, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (404, 1300, 2845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7727) [M::mem_pestat] mean and std.dev: (1744.43, 1666.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10168) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (312, 2184, 2598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7170) [M::mem_pestat] mean and std.dev: (1579.29, 1223.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9456) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (410, 1298, 3917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10931) [M::mem_pestat] mean and std.dev: (1992.14, 1973.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14438) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1023.034 CPU sec, 52.332 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1036344, 37, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (349, 696, 900) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2002) [M::mem_pestat] mean and std.dev: (603.56, 378.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.13, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (236, 1248, 3607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10349) [M::mem_pestat] mean and std.dev: (2429.14, 2734.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13720) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (771, 1503, 5168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13962) [M::mem_pestat] mean and std.dev: (2880.55, 2748.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18359) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1015.177 CPU sec, 44.814 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1037211, 35, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 443, 2330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6314) [M::mem_pestat] mean and std.dev: (1339.57, 1572.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8306) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.05, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1415, 2072, 3438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7484) [M::mem_pestat] mean and std.dev: (2049.44, 1335.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9507) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (672, 904, 1871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4269) [M::mem_pestat] mean and std.dev: (1144.11, 819.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5468) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1013.935 CPU sec, 49.885 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1037259, 33, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (360, 807, 2183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5829) [M::mem_pestat] mean and std.dev: (1141.20, 1165.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.05, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (288, 2188, 4831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13917) [M::mem_pestat] mean and std.dev: (2750.58, 2781.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18460) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (904, 1405, 3880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9832) [M::mem_pestat] mean and std.dev: (1764.00, 1540.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12808) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1015.663 CPU sec, 44.790 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 1035675, 30, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (351, 497, 1977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5229) [M::mem_pestat] mean and std.dev: (959.54, 1147.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (194, 1371, 2599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7409) [M::mem_pestat] mean and std.dev: (1584.40, 1574.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9814) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (814, 1095, 1865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3967) [M::mem_pestat] mean and std.dev: (1205.50, 557.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5018) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1038.031 CPU sec, 68.722 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036342, 41, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (313, 584, 1062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2560) [M::mem_pestat] mean and std.dev: (563.59, 363.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3309) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (489, 2238, 3716) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10170) [M::mem_pestat] mean and std.dev: (2827.02, 2844.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14205) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1259, 1985, 3396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7670) [M::mem_pestat] mean and std.dev: (2037.00, 1243.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9807) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1020.323 CPU sec, 47.741 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1037427, 25, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (373, 831, 2995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8239) [M::mem_pestat] mean and std.dev: (1647.32, 1773.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10861) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (115, 463, 2602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7576) [M::mem_pestat] mean and std.dev: (1437.52, 1742.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10063) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (583, 854, 5071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14047) [M::mem_pestat] mean and std.dev: (2111.00, 2295.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18535) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1016.906 CPU sec, 49.465 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1037221, 27, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (394, 819, 1509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3739) [M::mem_pestat] mean and std.dev: (972.90, 824.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1608, 2567, 3735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7989) [M::mem_pestat] mean and std.dev: (2549.16, 2011.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10596) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (558, 1217, 1561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3567) [M::mem_pestat] mean and std.dev: (981.38, 465.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4570) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1011.138 CPU sec, 44.634 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1036040, 23, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (418, 904, 2867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7765) [M::mem_pestat] mean and std.dev: (1880.67, 2087.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (381, 1079, 2944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8070) [M::mem_pestat] mean and std.dev: (1117.68, 994.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10633) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (679, 1675, 4082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10888) [M::mem_pestat] mean and std.dev: (2256.05, 2080.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14291) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1016.720 CPU sec, 52.241 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1036748, 39, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 388, 673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1551) [M::mem_pestat] mean and std.dev: (448.88, 405.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2073) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (327, 1583, 3569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10053) [M::mem_pestat] mean and std.dev: (2586.23, 2861.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14032) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1276, 1831, 3798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8842) [M::mem_pestat] mean and std.dev: (2882.93, 2513.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12938) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1012.810 CPU sec, 44.759 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036555, 25, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (378, 496, 2214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5886) [M::mem_pestat] mean and std.dev: (946.86, 1018.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (276, 2589, 2936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8256) [M::mem_pestat] mean and std.dev: (2137.48, 1797.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10916) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (933, 1431, 2815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6579) [M::mem_pestat] mean and std.dev: (1477.27, 1213.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8461) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1036.127 CPU sec, 68.975 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1036302, 38, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (368, 754, 3609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10091) [M::mem_pestat] mean and std.dev: (2000.81, 2487.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (286, 1888, 3585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10183) [M::mem_pestat] mean and std.dev: (2438.92, 2533.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13482) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1071, 1634, 2551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5511) [M::mem_pestat] mean and std.dev: (1718.95, 1277.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6991) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1020.993 CPU sec, 47.718 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1037454, 29, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 612, 997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2337) [M::mem_pestat] mean and std.dev: (540.61, 301.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3007) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (682, 2556, 4160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11116) [M::mem_pestat] mean and std.dev: (3164.14, 3080.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15488) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (530, 960, 1655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3905) [M::mem_pestat] mean and std.dev: (1092.63, 741.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5030) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1021.888 CPU sec, 53.665 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036005, 30, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (418, 755, 1422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3430) [M::mem_pestat] mean and std.dev: (710.18, 548.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4434) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (241, 1426, 3981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11461) [M::mem_pestat] mean and std.dev: (2735.30, 3044.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15201) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (809, 1143, 2304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5294) [M::mem_pestat] mean and std.dev: (1514.94, 997.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6789) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1017.461 CPU sec, 44.879 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1036745, 30, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (351, 417, 1377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3429) [M::mem_pestat] mean and std.dev: (689.61, 687.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (168, 1367, 5360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15744) [M::mem_pestat] mean and std.dev: (2713.47, 3177.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20936) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1064, 2589, 5031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12965) [M::mem_pestat] mean and std.dev: (3096.94, 2519.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16932) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1017.600 CPU sec, 49.423 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1035814, 29, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (347, 451, 2218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5960) [M::mem_pestat] mean and std.dev: (1001.56, 1254.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (982, 1877, 4952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12892) [M::mem_pestat] mean and std.dev: (2744.34, 2546.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (905, 2103, 3615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9035) [M::mem_pestat] mean and std.dev: (2130.30, 1437.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11745) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1017.648 CPU sec, 44.943 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1035773, 42, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (345, 763, 1114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2652) [M::mem_pestat] mean and std.dev: (734.30, 567.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.05, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (259, 1899, 6465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18877) [M::mem_pestat] mean and std.dev: (2959.07, 3215.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 25083) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (980, 2323, 4801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12443) [M::mem_pestat] mean and std.dev: (2703.94, 2246.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16264) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1041.073 CPU sec, 69.373 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1036932, 33, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (431, 743, 1262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2924) [M::mem_pestat] mean and std.dev: (854.56, 608.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1848, 2833, 5268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12108) [M::mem_pestat] mean and std.dev: (3446.36, 2692.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15528) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (906, 1247, 1659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3165) [M::mem_pestat] mean and std.dev: (1140.86, 561.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3918) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1021.522 CPU sec, 47.343 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1035890, 44, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (555, 1023, 4305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11805) [M::mem_pestat] mean and std.dev: (2500.84, 2704.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (263, 1902, 5451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15827) [M::mem_pestat] mean and std.dev: (2910.20, 3082.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21015) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (982, 1750, 6151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16489) [M::mem_pestat] mean and std.dev: (3365.47, 3008.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21658) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1025.629 CPU sec, 52.821 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 1035788, 26, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (842, 1734, 2828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6800) [M::mem_pestat] mean and std.dev: (1791.83, 1296.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (468, 2589, 4141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11487) [M::mem_pestat] mean and std.dev: (3040.58, 2940.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15160) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1151, 1311, 1507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (439, 2219) [M::mem_pestat] mean and std.dev: (1269.64, 276.85) [M::mem_pestat] low and high boundaries for proper pairs: (83, 2575) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1023.476 CPU sec, 45.435 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036455, 21, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (353, 498, 1240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3014) [M::mem_pestat] mean and std.dev: (593.05, 470.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.82) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (81, 377, 2917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8589) [M::mem_pestat] mean and std.dev: (1774.24, 2253.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11425) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (538, 1112, 3999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10921) [M::mem_pestat] mean and std.dev: (2545.56, 2520.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14382) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1017.037 CPU sec, 45.890 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1037112, 32, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (356, 809, 3787) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10649) [M::mem_pestat] mean and std.dev: (1993.30, 2447.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14080) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (204, 726, 3409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9819) [M::mem_pestat] mean and std.dev: (2282.47, 2822.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13571) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (855, 1703, 5957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16161) [M::mem_pestat] mean and std.dev: (2715.05, 2455.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21263) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1017.107 CPU sec, 44.842 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1037078, 30, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (402, 1428, 3412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9432) [M::mem_pestat] mean and std.dev: (2186.57, 2293.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (203, 1282, 3589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10361) [M::mem_pestat] mean and std.dev: (2497.57, 2864.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13955) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (713, 886, 2852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7130) [M::mem_pestat] mean and std.dev: (1615.27, 1361.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9269) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1014.463 CPU sec, 45.250 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1036213, 26, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (349, 478, 3052) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8458) [M::mem_pestat] mean and std.dev: (1270.35, 1403.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (296, 2055, 3981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11351) [M::mem_pestat] mean and std.dev: (2625.23, 2694.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15036) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1111, 1822, 2274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4600) [M::mem_pestat] mean and std.dev: (1493.29, 665.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5763) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1033.592 CPU sec, 69.317 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036717, 33, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (384, 906, 1238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2946) [M::mem_pestat] mean and std.dev: (774.50, 511.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (527, 1931, 3980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10886) [M::mem_pestat] mean and std.dev: (2663.67, 2518.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14339) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1020, 1529, 4371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11073) [M::mem_pestat] mean and std.dev: (2877.80, 2719.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14424) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1024.625 CPU sec, 48.036 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 1036440, 31, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (386, 723, 1771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4541) [M::mem_pestat] mean and std.dev: (742.42, 517.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5926) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (309, 1546, 2555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7047) [M::mem_pestat] mean and std.dev: (1516.17, 1288.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9293) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1025.898 CPU sec, 53.099 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1035741, 20, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (375, 653, 1793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4629) [M::mem_pestat] mean and std.dev: (848.68, 640.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6047) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (259, 1275, 3702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10588) [M::mem_pestat] mean and std.dev: (2544.65, 3152.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15153) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (604, 1037, 3113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8131) [M::mem_pestat] mean and std.dev: (1804.00, 1760.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10640) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1019.512 CPU sec, 45.022 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 1036468, 30, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (399, 481, 1971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5115) [M::mem_pestat] mean and std.dev: (901.64, 871.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (133, 985, 3481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10177) [M::mem_pestat] mean and std.dev: (2607.83, 3246.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15592) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (752, 1608, 1918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4250) [M::mem_pestat] mean and std.dev: (1610.71, 1122.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6101) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1017.082 CPU sec, 50.282 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1035943, 36, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (345, 697, 1370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3420) [M::mem_pestat] mean and std.dev: (829.04, 630.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (405, 2163, 2597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6981) [M::mem_pestat] mean and std.dev: (1772.21, 1498.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9173) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (826, 1255, 1877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3979) [M::mem_pestat] mean and std.dev: (1195.89, 662.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5030) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1017.912 CPU sec, 44.951 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 1036310, 28, 19) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (343, 1935, 2915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8059) [M::mem_pestat] mean and std.dev: (1641.12, 1541.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10631) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (991, 1606, 5352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14074) [M::mem_pestat] mean and std.dev: (2871.58, 2622.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18435) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1046.155 CPU sec, 68.480 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 1036564, 23, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (372, 948, 2746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7494) [M::mem_pestat] mean and std.dev: (1315.62, 1193.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9868) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (256, 2092, 2931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8281) [M::mem_pestat] mean and std.dev: (1813.67, 1536.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10956) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (651, 1220, 2720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6858) [M::mem_pestat] mean and std.dev: (1672.16, 1586.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8927) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1028.627 CPU sec, 48.210 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1035739, 30, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (385, 849, 2149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5677) [M::mem_pestat] mean and std.dev: (1254.94, 1048.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (160, 586, 1999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5677) [M::mem_pestat] mean and std.dev: (1059.93, 1412.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7516) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1025.250 CPU sec, 52.455 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036964, 31, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (448, 985, 1789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4471) [M::mem_pestat] mean and std.dev: (1113.83, 987.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5812) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (620, 1250, 4143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11189) [M::mem_pestat] mean and std.dev: (2555.81, 2814.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14712) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (404, 1065, 1913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4931) [M::mem_pestat] mean and std.dev: (1320.80, 1037.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6440) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1028.583 CPU sec, 45.406 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 1037724, 31, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (447, 1290, 1932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4902) [M::mem_pestat] mean and std.dev: (1266.76, 1028.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (449, 2421, 3416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9350) [M::mem_pestat] mean and std.dev: (2595.97, 2508.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12629) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (348, 1226, 2756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7572) [M::mem_pestat] mean and std.dev: (1343.20, 1147.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9980) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1019.041 CPU sec, 50.098 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036520, 39, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (374, 707, 1499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3749) [M::mem_pestat] mean and std.dev: (689.86, 470.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (248, 762, 4093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11783) [M::mem_pestat] mean and std.dev: (2534.67, 3096.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15628) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (827, 1273, 3612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9182) [M::mem_pestat] mean and std.dev: (2114.47, 1687.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11967) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1017.177 CPU sec, 44.784 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1035911, 32, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (404, 978, 1307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3113) [M::mem_pestat] mean and std.dev: (1054.88, 789.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4211) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (142, 1048, 2860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8296) [M::mem_pestat] mean and std.dev: (1448.94, 1436.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11014) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (465, 1665, 5098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14364) [M::mem_pestat] mean and std.dev: (2782.47, 2709.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18997) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1037.625 CPU sec, 69.128 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1035671, 31, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (390, 1080, 1458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3594) [M::mem_pestat] mean and std.dev: (934.78, 571.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (314, 1292, 3561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10055) [M::mem_pestat] mean and std.dev: (2854.23, 3231.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15781) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (351, 701, 1408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3522) [M::mem_pestat] mean and std.dev: (672.08, 503.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4579) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1020.828 CPU sec, 47.585 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036848, 30, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 538, 1886) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5128) [M::mem_pestat] mean and std.dev: (824.86, 984.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (160, 2584, 3722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10846) [M::mem_pestat] mean and std.dev: (2948.67, 3047.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15137) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (787, 1889, 2521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5989) [M::mem_pestat] mean and std.dev: (1691.94, 1045.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7723) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1021.706 CPU sec, 50.765 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1037591, 24, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (426, 910, 1855) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4713) [M::mem_pestat] mean and std.dev: (1088.47, 1010.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6142) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (345, 806, 2595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7095) [M::mem_pestat] mean and std.dev: (1561.12, 1404.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9345) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (944, 1115, 3456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8480) [M::mem_pestat] mean and std.dev: (1920.25, 1970.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10992) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1012.234 CPU sec, 44.629 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 1036444, 41, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (354, 458, 1354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3354) [M::mem_pestat] mean and std.dev: (800.89, 734.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (778, 2587, 2904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7156) [M::mem_pestat] mean and std.dev: (1887.03, 1320.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9282) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (869, 1392, 1722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3428) [M::mem_pestat] mean and std.dev: (1208.17, 447.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4281) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1018.948 CPU sec, 49.468 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1035863, 26, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (379, 1008, 1944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5074) [M::mem_pestat] mean and std.dev: (1343.71, 1268.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.05, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (245, 1406, 3992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11486) [M::mem_pestat] mean and std.dev: (2408.65, 2785.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15233) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (937, 1343, 1661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3109) [M::mem_pestat] mean and std.dev: (1163.42, 530.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3833) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1016.431 CPU sec, 44.763 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 1036184, 26, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (354, 429, 1980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5232) [M::mem_pestat] mean and std.dev: (1075.42, 1089.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (397, 2604, 3992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11182) [M::mem_pestat] mean and std.dev: (3001.65, 2753.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14777) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (689, 1339, 1836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4130) [M::mem_pestat] mean and std.dev: (1205.95, 730.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5277) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1040.921 CPU sec, 69.151 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1037301, 30, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (361, 381, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (197, 607) [M::mem_pestat] mean and std.dev: (381.47, 73.20) [M::mem_pestat] low and high boundaries for proper pairs: (89, 689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (395, 2279, 2879) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7847) [M::mem_pestat] mean and std.dev: (1766.48, 1362.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10331) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (811, 1394, 1826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3856) [M::mem_pestat] mean and std.dev: (1281.32, 833.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4871) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1021.269 CPU sec, 47.586 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036172, 35, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (409, 670, 2711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7315) [M::mem_pestat] mean and std.dev: (1425.40, 1528.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.05, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (379, 1830, 2852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7798) [M::mem_pestat] mean and std.dev: (1606.32, 1772.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10271) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (438, 1467, 3433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9423) [M::mem_pestat] mean and std.dev: (2194.80, 2470.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12418) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1019.141 CPU sec, 48.840 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036112, 27, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (618, 1323, 2058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4938) [M::mem_pestat] mean and std.dev: (1466.41, 1006.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6378) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (866, 2598, 4812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12704) [M::mem_pestat] mean and std.dev: (3256.81, 2970.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16650) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (893, 1590, 8082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22460) [M::mem_pestat] mean and std.dev: (3063.33, 3290.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 29649) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1012.959 CPU sec, 44.704 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1037453, 31, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (394, 1479, 3798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10606) [M::mem_pestat] mean and std.dev: (2487.27, 2782.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14010) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (534, 2564, 3913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10671) [M::mem_pestat] mean and std.dev: (2988.00, 2729.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14050) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (977, 1850, 2432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5342) [M::mem_pestat] mean and std.dev: (1576.38, 970.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6797) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1019.609 CPU sec, 48.422 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036671, 30, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (352, 567, 4691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13369) [M::mem_pestat] mean and std.dev: (2297.88, 2638.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (778, 2575, 4805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12859) [M::mem_pestat] mean and std.dev: (3338.93, 2895.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16886) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1111, 1320, 3016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6826) [M::mem_pestat] mean and std.dev: (1787.31, 1319.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8731) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1016.357 CPU sec, 44.917 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1035391, 31, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (385, 775, 1496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3718) [M::mem_pestat] mean and std.dev: (948.75, 704.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (175, 1869, 3598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10444) [M::mem_pestat] mean and std.dev: (2401.84, 2474.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13867) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (404, 1408, 6347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18233) [M::mem_pestat] mean and std.dev: (2641.92, 2766.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 24176) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1027.367 CPU sec, 47.392 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036272, 28, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 408, 1089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2599) [M::mem_pestat] mean and std.dev: (571.23, 528.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (336, 1262, 4137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11739) [M::mem_pestat] mean and std.dev: (2189.36, 2343.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15540) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (458, 1164, 1684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4136) [M::mem_pestat] mean and std.dev: (979.50, 551.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5362) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1042.746 CPU sec, 68.833 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036981, 31, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (366, 427, 1179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2805) [M::mem_pestat] mean and std.dev: (578.95, 412.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (271, 1588, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7276) [M::mem_pestat] mean and std.dev: (1257.28, 1127.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9611) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (853, 1494, 6487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17755) [M::mem_pestat] mean and std.dev: (3124.44, 3085.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23389) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1026.489 CPU sec, 47.933 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 1036050, 26, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 548, 1483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3795) [M::mem_pestat] mean and std.dev: (833.27, 758.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4951) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (68, 1162, 3210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9494) [M::mem_pestat] mean and std.dev: (1999.85, 2412.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12636) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (790, 1035, 1778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3754) [M::mem_pestat] mean and std.dev: (1165.47, 757.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4742) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1023.078 CPU sec, 52.148 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1036825, 34, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 830, 2009) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5377) [M::mem_pestat] mean and std.dev: (1123.10, 1195.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (460, 2434, 4298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11974) [M::mem_pestat] mean and std.dev: (3157.09, 3106.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15812) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (418, 1304, 3953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11023) [M::mem_pestat] mean and std.dev: (2351.80, 2510.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14558) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1017.254 CPU sec, 44.905 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036373, 25, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (404, 1465, 2705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7307) [M::mem_pestat] mean and std.dev: (1698.23, 1967.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (278, 2502, 8279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24281) [M::mem_pestat] mean and std.dev: (3370.28, 3320.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 32282) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1036, 1250, 2034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4030) [M::mem_pestat] mean and std.dev: (1362.10, 568.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5028) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1014.535 CPU sec, 48.172 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1037042, 39, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 442, 1451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3725) [M::mem_pestat] mean and std.dev: (1001.38, 971.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4889) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.12, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (365, 1878, 2593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7049) [M::mem_pestat] mean and std.dev: (1753.14, 1479.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9277) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1015.180 CPU sec, 44.750 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1036423, 27, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 681, 2846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8008) [M::mem_pestat] mean and std.dev: (1599.04, 1909.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.12, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1539, 2605, 6414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16164) [M::mem_pestat] mean and std.dev: (3687.22, 2903.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21039) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (991, 1284, 1967) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3919) [M::mem_pestat] mean and std.dev: (1137.31, 359.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4895) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1039.196 CPU sec, 69.299 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1037302, 42, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (291, 351, 4251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12171) [M::mem_pestat] mean and std.dev: (2066.86, 2609.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16131) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (277, 2595, 5364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15538) [M::mem_pestat] mean and std.dev: (3106.64, 2836.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20625) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (494, 1295, 1692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4088) [M::mem_pestat] mean and std.dev: (1140.75, 938.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5286) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1020.934 CPU sec, 47.563 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036918, 22, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (412, 1403, 2216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5824) [M::mem_pestat] mean and std.dev: (1539.21, 1214.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (708, 1903, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6402) [M::mem_pestat] mean and std.dev: (1671.90, 1263.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8300) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (954, 1254, 2301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4995) [M::mem_pestat] mean and std.dev: (1404.07, 849.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6342) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1023.400 CPU sec, 50.901 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 1036206, 31, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 929, 1833) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4819) [M::mem_pestat] mean and std.dev: (1121.16, 1205.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6312) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.05, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (521, 1672, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6776) [M::mem_pestat] mean and std.dev: (1428.85, 1102.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8861) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (940, 1502, 2302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5026) [M::mem_pestat] mean and std.dev: (1451.16, 993.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6388) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1024.762 CPU sec, 45.250 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 1036218, 39, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 879, 3613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10215) [M::mem_pestat] mean and std.dev: (2036.79, 2392.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (205, 1567, 6896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 20278) [M::mem_pestat] mean and std.dev: (2940.74, 3360.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 26969) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1033, 1615, 2467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5335) [M::mem_pestat] mean and std.dev: (1664.22, 1094.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6769) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1013.898 CPU sec, 50.206 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 1036888, 16, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (855, 1768, 3569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8997) [M::mem_pestat] mean and std.dev: (2177.54, 1662.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.05, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (831, 2086, 2590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6108) [M::mem_pestat] mean and std.dev: (1724.07, 1237.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7867) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (910, 1568, 2987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7141) [M::mem_pestat] mean and std.dev: (1853.73, 1286.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9218) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1014.761 CPU sec, 44.754 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036394, 27, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (409, 859, 1689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4249) [M::mem_pestat] mean and std.dev: (972.74, 740.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (205, 1358, 3775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10915) [M::mem_pestat] mean and std.dev: (2427.52, 2682.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14485) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1114, 1617, 1980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3712) [M::mem_pestat] mean and std.dev: (1240.50, 563.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4578) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1043.333 CPU sec, 69.708 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 1036650, 28, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (361, 1174, 4824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13750) [M::mem_pestat] mean and std.dev: (2431.83, 2689.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18213) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (380, 1873, 7371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 21353) [M::mem_pestat] mean and std.dev: (3195.11, 3476.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 28344) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1043, 1138, 5393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14093) [M::mem_pestat] mean and std.dev: (2534.50, 2403.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18443) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1021.135 CPU sec, 47.689 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 1036515, 24, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (393, 583, 1513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3753) [M::mem_pestat] mean and std.dev: (863.93, 712.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (202, 1434, 8497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 25087) [M::mem_pestat] mean and std.dev: (3140.54, 3624.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 33382) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (894, 1678, 2435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5517) [M::mem_pestat] mean and std.dev: (1452.53, 815.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7058) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1021.530 CPU sec, 52.154 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 1037513, 36, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (356, 432, 1556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3956) [M::mem_pestat] mean and std.dev: (728.56, 767.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5156) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (191, 1534, 5762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16904) [M::mem_pestat] mean and std.dev: (2781.83, 2968.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22475) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (778, 1237, 3898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10138) [M::mem_pestat] mean and std.dev: (2118.92, 2395.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13258) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1011.225 CPU sec, 44.576 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1036078, 42, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (341, 636, 1811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4751) [M::mem_pestat] mean and std.dev: (906.41, 989.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6221) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (250, 2271, 3958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11374) [M::mem_pestat] mean and std.dev: (2753.50, 2939.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15082) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (501, 1047, 5320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14958) [M::mem_pestat] mean and std.dev: (2467.33, 2720.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19777) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1020.469 CPU sec, 50.778 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1036663, 37, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (309, 590, 1417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3633) [M::mem_pestat] mean and std.dev: (869.29, 906.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (227, 1305, 2596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7334) [M::mem_pestat] mean and std.dev: (1394.76, 1440.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9703) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (776, 1296, 2676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6476) [M::mem_pestat] mean and std.dev: (1599.45, 1363.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8376) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1019.380 CPU sec, 44.913 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1037195, 27, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (359, 1617, 2176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5810) [M::mem_pestat] mean and std.dev: (1533.95, 1294.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (2085, 2612, 3861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7413) [M::mem_pestat] mean and std.dev: (2797.43, 1477.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9189) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (329, 1265, 1461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3725) [M::mem_pestat] mean and std.dev: (966.23, 630.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4857) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1033.189 CPU sec, 68.769 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1035609, 32, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (380, 645, 1949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5087) [M::mem_pestat] mean and std.dev: (1042.14, 1060.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (251, 1849, 2948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8342) [M::mem_pestat] mean and std.dev: (1909.74, 1860.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11039) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (672, 2030, 4286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11514) [M::mem_pestat] mean and std.dev: (2818.18, 2741.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15128) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1018.471 CPU sec, 47.619 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 1035771, 29, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (346, 394, 1481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3751) [M::mem_pestat] mean and std.dev: (792.04, 782.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4886) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (240, 1065, 2590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7290) [M::mem_pestat] mean and std.dev: (1221.93, 1178.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9640) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1152, 1676, 4259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10473) [M::mem_pestat] mean and std.dev: (2784.21, 2406.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13580) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1017.426 CPU sec, 48.407 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1035957, 27, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (652, 1179, 1861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4279) [M::mem_pestat] mean and std.dev: (1255.53, 888.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (237, 1017, 3611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10359) [M::mem_pestat] mean and std.dev: (2534.52, 2868.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14008) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (840, 1050, 2448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5664) [M::mem_pestat] mean and std.dev: (1135.78, 718.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7272) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1015.989 CPU sec, 44.820 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1036450, 39, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (416, 1645, 3471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9581) [M::mem_pestat] mean and std.dev: (2159.86, 2278.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (119, 394, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7583) [M::mem_pestat] mean and std.dev: (1271.06, 1761.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10071) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1395, 1516, 1710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (765, 2340) [M::mem_pestat] mean and std.dev: (1519.00, 115.38) [M::mem_pestat] low and high boundaries for proper pairs: (450, 2655) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1011.952 CPU sec, 48.274 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1036608, 29, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (423, 906, 2350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6204) [M::mem_pestat] mean and std.dev: (1306.00, 1305.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8131) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (157, 1480, 2902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8392) [M::mem_pestat] mean and std.dev: (1705.89, 1969.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11137) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (797, 2247, 3081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7649) [M::mem_pestat] mean and std.dev: (1920.31, 1488.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9933) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1009.578 CPU sec, 44.473 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036880, 26, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (394, 525, 1006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2230) [M::mem_pestat] mean and std.dev: (594.15, 404.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.88) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (83, 820, 1874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5456) [M::mem_pestat] mean and std.dev: (990.42, 1131.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7247) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (911, 1191, 1300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (133, 2078) [M::mem_pestat] mean and std.dev: (1021.40, 430.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2743) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1012.645 CPU sec, 48.371 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036772, 38, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (390, 1324, 2169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5727) [M::mem_pestat] mean and std.dev: (1138.13, 1002.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (300, 2547, 3345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9435) [M::mem_pestat] mean and std.dev: (2746.87, 2783.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13880) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1025.742 CPU sec, 68.120 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1037006, 28, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (431, 1027, 2860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7718) [M::mem_pestat] mean and std.dev: (1852.33, 1881.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10147) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (286, 1861, 4106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11746) [M::mem_pestat] mean and std.dev: (2824.25, 3143.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15566) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (792, 1802, 2950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7266) [M::mem_pestat] mean and std.dev: (2158.56, 1843.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9534) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1015.048 CPU sec, 47.358 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1036082, 36, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (304, 589, 1972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5308) [M::mem_pestat] mean and std.dev: (1099.18, 1270.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6976) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (215, 1433, 3519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10127) [M::mem_pestat] mean and std.dev: (2306.83, 2639.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13431) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1280, 1883, 2138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3854) [M::mem_pestat] mean and std.dev: (1401.70, 660.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4712) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1011.722 CPU sec, 45.255 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1036280, 27, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 433, 1319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3445) [M::mem_pestat] mean and std.dev: (809.59, 874.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (202, 2249, 4645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13531) [M::mem_pestat] mean and std.dev: (2772.15, 2999.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17974) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1345, 1676, 3511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7843) [M::mem_pestat] mean and std.dev: (1958.25, 1474.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10009) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1006.174 CPU sec, 44.320 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036192, 22, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (313, 861, 1880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5014) [M::mem_pestat] mean and std.dev: (977.06, 1077.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (649, 2585, 3602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9508) [M::mem_pestat] mean and std.dev: (2919.14, 2463.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12773) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (472, 988, 3000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8056) [M::mem_pestat] mean and std.dev: (1434.31, 1121.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10584) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1010.259 CPU sec, 47.608 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1036297, 36, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (358, 1036, 1379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3421) [M::mem_pestat] mean and std.dev: (904.94, 863.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (296, 1621, 3975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11333) [M::mem_pestat] mean and std.dev: (2532.50, 2750.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15012) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1008.185 CPU sec, 44.477 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036598, 32, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (332, 407, 1356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3404) [M::mem_pestat] mean and std.dev: (621.11, 631.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (227, 1910, 2613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7385) [M::mem_pestat] mean and std.dev: (1606.97, 1515.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9771) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1445, 3387, 6662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17096) [M::mem_pestat] mean and std.dev: (3805.25, 3116.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22313) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1026.828 CPU sec, 67.041 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 1036309, 28, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (333, 693, 1784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4686) [M::mem_pestat] mean and std.dev: (955.50, 970.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6137) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1354, 1991, 3410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7522) [M::mem_pestat] mean and std.dev: (2081.44, 1523.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9578) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (2340, 3766, 6129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13707) [M::mem_pestat] mean and std.dev: (3830.36, 2373.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17496) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1015.250 CPU sec, 47.318 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036329, 36, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (363, 420, 1493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3753) [M::mem_pestat] mean and std.dev: (980.94, 979.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4897) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.12, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (404, 2592, 7164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 20684) [M::mem_pestat] mean and std.dev: (3552.25, 3197.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 27444) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (759, 1303, 4356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11550) [M::mem_pestat] mean and std.dev: (2308.17, 2137.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15147) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1006.282 CPU sec, 45.347 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1036752, 36, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (382, 470, 1028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2320) [M::mem_pestat] mean and std.dev: (698.73, 543.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2966) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (543, 2054, 3574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9636) [M::mem_pestat] mean and std.dev: (2732.97, 2769.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13812) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (621, 847, 3518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9312) [M::mem_pestat] mean and std.dev: (1415.17, 1229.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12209) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1011.185 CPU sec, 44.568 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1036704, 19, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (387, 1517, 3124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8598) [M::mem_pestat] mean and std.dev: (1798.06, 1799.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11335) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (619, 2611, 4008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10786) [M::mem_pestat] mean and std.dev: (3081.00, 2966.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14948) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1005.744 CPU sec, 46.138 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1035786, 25, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (390, 866, 1304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3132) [M::mem_pestat] mean and std.dev: (736.87, 502.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4046) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (362, 1294, 3401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9479) [M::mem_pestat] mean and std.dev: (2173.52, 2264.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12518) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (633, 1150, 1763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4023) [M::mem_pestat] mean and std.dev: (1162.80, 664.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5153) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1012.707 CPU sec, 44.645 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1035992, 41, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (322, 772, 1482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3802) [M::mem_pestat] mean and std.dev: (775.42, 505.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4962) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (645, 2005, 3733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9909) [M::mem_pestat] mean and std.dev: (2815.17, 2740.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13776) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1025.978 CPU sec, 66.420 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1035419, 40, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 441, 1106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2642) [M::mem_pestat] mean and std.dev: (552.28, 501.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1005, 1881, 4631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11883) [M::mem_pestat] mean and std.dev: (3037.95, 2913.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15509) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (506, 1888, 5307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14909) [M::mem_pestat] mean and std.dev: (2810.84, 2807.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19710) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1018.302 CPU sec, 47.502 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 1036568, 39, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (364, 776, 1764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4564) [M::mem_pestat] mean and std.dev: (985.04, 1040.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5964) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (234, 1865, 3602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10338) [M::mem_pestat] mean and std.dev: (2758.51, 2946.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14544) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1083, 1216, 2086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4092) [M::mem_pestat] mean and std.dev: (1236.40, 600.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1009.774 CPU sec, 49.711 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 1036732, 38, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (673, 1116, 2150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5104) [M::mem_pestat] mean and std.dev: (1106.83, 813.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (177, 2046, 3644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10578) [M::mem_pestat] mean and std.dev: (2800.47, 3005.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14822) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (900, 1354, 2230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4890) [M::mem_pestat] mean and std.dev: (1523.47, 916.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6220) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1004.358 CPU sec, 44.242 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036722, 29, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 486, 1423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3589) [M::mem_pestat] mean and std.dev: (818.83, 718.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (300, 1937, 4260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12180) [M::mem_pestat] mean and std.dev: (2849.52, 3019.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16140) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1092, 2371, 6174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16338) [M::mem_pestat] mean and std.dev: (3134.41, 2621.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21420) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1006.999 CPU sec, 46.657 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1036349, 32, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 398, 1215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3021) [M::mem_pestat] mean and std.dev: (556.94, 420.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3924) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.12, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (475, 1452, 2951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7903) [M::mem_pestat] mean and std.dev: (1653.38, 1542.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10379) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (747, 1156, 1608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3330) [M::mem_pestat] mean and std.dev: (1231.22, 798.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4426) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1007.982 CPU sec, 44.408 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 1036811, 25, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 415, 1087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2903) [M::mem_pestat] mean and std.dev: (621.54, 628.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (330, 2033, 8492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24816) [M::mem_pestat] mean and std.dev: (3100.64, 3275.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 32978) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1026, 1457, 2514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5490) [M::mem_pestat] mean and std.dev: (1588.31, 779.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6978) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1025.722 CPU sec, 67.997 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036142, 32, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (385, 1643, 2440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6550) [M::mem_pestat] mean and std.dev: (1935.65, 1844.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9313) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.12, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (201, 1233, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7416) [M::mem_pestat] mean and std.dev: (1221.62, 1303.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9821) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (420, 1616, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6969) [M::mem_pestat] mean and std.dev: (1022.77, 804.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9152) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1011.682 CPU sec, 47.045 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 1036372, 27, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (366, 911, 2356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6336) [M::mem_pestat] mean and std.dev: (1163.76, 1121.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8326) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (631, 2149, 3822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10204) [M::mem_pestat] mean and std.dev: (2701.26, 2606.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13395) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1173, 1514, 2969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6561) [M::mem_pestat] mean and std.dev: (1641.00, 1204.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8357) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1010.556 CPU sec, 47.981 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1037126, 28, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (378, 1350, 1902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4950) [M::mem_pestat] mean and std.dev: (1234.25, 1070.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (449, 1571, 3870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10712) [M::mem_pestat] mean and std.dev: (2427.46, 2489.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14133) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (837, 1427, 1961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4209) [M::mem_pestat] mean and std.dev: (1173.90, 657.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5333) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1007.838 CPU sec, 44.532 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1036228, 44, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (349, 407, 2270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6112) [M::mem_pestat] mean and std.dev: (858.73, 944.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8033) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (418, 2000, 3909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10891) [M::mem_pestat] mean and std.dev: (2734.32, 2755.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14382) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (393, 950, 1631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4107) [M::mem_pestat] mean and std.dev: (952.56, 580.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5345) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1007.364 CPU sec, 48.511 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1037002, 33, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (368, 1124, 2153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5723) [M::mem_pestat] mean and std.dev: (1393.09, 1429.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (397, 1919, 2869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7813) [M::mem_pestat] mean and std.dev: (1977.53, 1891.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10285) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1508, 1837, 2847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5525) [M::mem_pestat] mean and std.dev: (1949.58, 795.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6864) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1008.982 CPU sec, 44.492 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1036431, 28, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (812, 1320, 1997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4367) [M::mem_pestat] mean and std.dev: (1243.36, 651.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (828, 2576, 3593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9123) [M::mem_pestat] mean and std.dev: (2730.36, 2412.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12378) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (923, 1323, 2242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4880) [M::mem_pestat] mean and std.dev: (1523.53, 1003.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6199) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1006.773 CPU sec, 47.089 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1036667, 35, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (427, 1322, 3098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8440) [M::mem_pestat] mean and std.dev: (1592.53, 1504.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11111) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (299, 1761, 3991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11375) [M::mem_pestat] mean and std.dev: (2729.17, 2783.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15067) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (807, 1231, 2042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4512) [M::mem_pestat] mean and std.dev: (1522.57, 1109.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5960) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1023.362 CPU sec, 66.544 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036342, 37, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (396, 564, 1734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4410) [M::mem_pestat] mean and std.dev: (906.53, 832.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (345, 2567, 3623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10179) [M::mem_pestat] mean and std.dev: (2719.68, 2593.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13457) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1587, 3635, 4160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9306) [M::mem_pestat] mean and std.dev: (3039.64, 1988.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11879) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1008.652 CPU sec, 47.311 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 1036583, 31, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (381, 971, 2036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5346) [M::mem_pestat] mean and std.dev: (1260.93, 990.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7001) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (117, 1273, 3431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10059) [M::mem_pestat] mean and std.dev: (2428.26, 3024.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14527) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (685, 1068, 1732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3826) [M::mem_pestat] mean and std.dev: (1043.27, 598.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4873) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1014.622 CPU sec, 50.264 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 1036795, 25, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (623, 974, 2234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5456) [M::mem_pestat] mean and std.dev: (1342.00, 917.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7067) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (383, 2194, 3809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10661) [M::mem_pestat] mean and std.dev: (2465.12, 2502.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14087) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (787, 1194, 4248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11170) [M::mem_pestat] mean and std.dev: (2230.53, 2278.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14631) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1009.027 CPU sec, 44.641 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1036464, 37, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (374, 668, 1785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4607) [M::mem_pestat] mean and std.dev: (849.04, 634.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6018) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (224, 1215, 4813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13991) [M::mem_pestat] mean and std.dev: (2835.95, 3193.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18580) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1124, 1420, 1734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2954) [M::mem_pestat] mean and std.dev: (1311.53, 404.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3564) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1011.381 CPU sec, 50.603 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1035388, 32, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (354, 486, 1548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3936) [M::mem_pestat] mean and std.dev: (904.35, 856.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5130) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (162, 1459, 2590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7446) [M::mem_pestat] mean and std.dev: (1359.93, 1310.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9874) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (427, 1499, 2250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5896) [M::mem_pestat] mean and std.dev: (1550.89, 1085.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7719) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1012.279 CPU sec, 44.621 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 1036118, 39, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (364, 438, 1466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3670) [M::mem_pestat] mean and std.dev: (758.25, 810.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (277, 1089, 2577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7177) [M::mem_pestat] mean and std.dev: (1221.79, 1155.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (831, 1182, 1647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3279) [M::mem_pestat] mean and std.dev: (1157.36, 633.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1019.456 CPU sec, 66.805 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1036301, 27, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (378, 439, 2147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5685) [M::mem_pestat] mean and std.dev: (1155.81, 1222.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (344, 2560, 3890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10982) [M::mem_pestat] mean and std.dev: (2664.00, 2621.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14528) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1029, 1957, 3662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8928) [M::mem_pestat] mean and std.dev: (2339.27, 1791.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11561) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1012.824 CPU sec, 47.199 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1037336, 22, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (387, 904, 2560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6906) [M::mem_pestat] mean and std.dev: (1464.59, 1388.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9079) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (519, 1893, 8254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 23724) [M::mem_pestat] mean and std.dev: (3261.36, 3327.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 31459) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (638, 1135, 1349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2771) [M::mem_pestat] mean and std.dev: (1122.73, 730.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4043) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1002.992 CPU sec, 50.032 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1036444, 41, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (313, 750, 2837) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7885) [M::mem_pestat] mean and std.dev: (1663.69, 1703.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (254, 2526, 5524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16064) [M::mem_pestat] mean and std.dev: (3068.12, 3238.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21334) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1879, 2736, 3055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5407) [M::mem_pestat] mean and std.dev: (2330.77, 983.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6583) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1003.605 CPU sec, 44.221 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1035416, 29, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (344, 385, 1148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2756) [M::mem_pestat] mean and std.dev: (609.83, 442.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (806, 2087, 2830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6878) [M::mem_pestat] mean and std.dev: (1966.64, 1330.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8902) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (481, 1244, 2096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5326) [M::mem_pestat] mean and std.dev: (1452.21, 1156.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6941) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1008.567 CPU sec, 47.776 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1035488, 33, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 817, 2065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5541) [M::mem_pestat] mean and std.dev: (1109.17, 1255.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7279) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (241, 1883, 3670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10528) [M::mem_pestat] mean and std.dev: (2509.36, 2516.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13957) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1004, 1481, 6367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17093) [M::mem_pestat] mean and std.dev: (2944.33, 3136.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22456) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1008.291 CPU sec, 44.366 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 1036719, 34, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 367, 1748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4634) [M::mem_pestat] mean and std.dev: (692.23, 758.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6077) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (221, 1471, 2903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8267) [M::mem_pestat] mean and std.dev: (1285.41, 1390.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10949) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (777, 1417, 2155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4911) [M::mem_pestat] mean and std.dev: (1526.71, 960.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6289) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1021.834 CPU sec, 66.966 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1035770, 17, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (358, 424, 2676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7312) [M::mem_pestat] mean and std.dev: (1352.29, 1524.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1624, 2594, 7322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18718) [M::mem_pestat] mean and std.dev: (3737.65, 2937.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 24416) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1089, 1554, 2373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4941) [M::mem_pestat] mean and std.dev: (1277.75, 580.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6225) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1012.891 CPU sec, 47.110 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036002, 38, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (422, 1016, 1598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3950) [M::mem_pestat] mean and std.dev: (1041.16, 669.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5126) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (355, 1880, 2574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7012) [M::mem_pestat] mean and std.dev: (1560.34, 1385.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9231) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (873, 1589, 4671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12267) [M::mem_pestat] mean and std.dev: (2295.77, 1800.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16065) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1007.030 CPU sec, 48.275 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 1036042, 44, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (365, 466, 1505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3785) [M::mem_pestat] mean and std.dev: (983.77, 973.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4925) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (297, 2566, 5090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14676) [M::mem_pestat] mean and std.dev: (3131.70, 3142.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19469) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1142, 1823, 3598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8510) [M::mem_pestat] mean and std.dev: (2031.92, 1380.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10966) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1005.553 CPU sec, 44.317 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036317, 38, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (406, 560, 1346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3226) [M::mem_pestat] mean and std.dev: (834.35, 680.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4166) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (211, 1848, 2631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7471) [M::mem_pestat] mean and std.dev: (1591.12, 1316.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9891) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1048, 1523, 2522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5470) [M::mem_pestat] mean and std.dev: (1795.47, 1230.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6944) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1007.169 CPU sec, 47.527 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1035970, 31, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (326, 452, 737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1559) [M::mem_pestat] mean and std.dev: (507.06, 313.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1970) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.12, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (269, 1847, 3912) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11198) [M::mem_pestat] mean and std.dev: (2493.19, 2567.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14841) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (967, 1312, 2342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5092) [M::mem_pestat] mean and std.dev: (1749.00, 1285.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6892) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1009.062 CPU sec, 44.452 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1036824, 27, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (411, 931, 1786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4536) [M::mem_pestat] mean and std.dev: (1046.40, 784.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (200, 2586, 2811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8033) [M::mem_pestat] mean and std.dev: (1680.04, 1449.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10644) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1025, 1410, 4153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10409) [M::mem_pestat] mean and std.dev: (2608.43, 2511.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13537) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1025.408 CPU sec, 66.533 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1035779, 22, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 690, 1166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3190) [M::mem_pestat] mean and std.dev: (693.74, 708.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4202) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.04, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (238, 1412, 3631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10417) [M::mem_pestat] mean and std.dev: (2563.64, 2785.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13810) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (438, 1139, 2928) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7908) [M::mem_pestat] mean and std.dev: (1847.90, 2009.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10398) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1015.849 CPU sec, 47.691 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 1036118, 20, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (411, 765, 2681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7221) [M::mem_pestat] mean and std.dev: (1432.58, 1901.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1153, 2522, 6363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16783) [M::mem_pestat] mean and std.dev: (3263.65, 2905.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21993) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (502, 1000, 1936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4804) [M::mem_pestat] mean and std.dev: (953.13, 523.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6238) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1008.967 CPU sec, 49.571 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036644, 32, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (396, 734, 3850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10758) [M::mem_pestat] mean and std.dev: (2304.62, 2977.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14216) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (250, 446, 2599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7297) [M::mem_pestat] mean and std.dev: (1232.17, 1448.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9646) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (921, 1250, 2277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4989) [M::mem_pestat] mean and std.dev: (1183.62, 505.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6345) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1003.593 CPU sec, 44.240 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036909, 43, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 401, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (315.73, 182.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.13, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (496, 1871, 4830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13498) [M::mem_pestat] mean and std.dev: (3123.93, 3116.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17832) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (811, 1811, 2514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5920) [M::mem_pestat] mean and std.dev: (1562.67, 1120.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7623) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1011.521 CPU sec, 49.627 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1037368, 38, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (370, 615, 1677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4291) [M::mem_pestat] mean and std.dev: (945.33, 971.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (311, 1194, 2596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7166) [M::mem_pestat] mean and std.dev: (1185.27, 1067.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9451) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1000, 1725, 5718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15154) [M::mem_pestat] mean and std.dev: (3020.47, 2806.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19872) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1008.588 CPU sec, 44.438 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1036944, 36, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (350, 438, 1563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3989) [M::mem_pestat] mean and std.dev: (814.44, 807.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5202) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (949, 2579, 3602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8908) [M::mem_pestat] mean and std.dev: (2787.83, 2604.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13208) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (561, 1066, 3527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9459) [M::mem_pestat] mean and std.dev: (2355.00, 2484.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12425) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1010.175 CPU sec, 48.434 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 1036889, 31, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (403, 899, 3499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9691) [M::mem_pestat] mean and std.dev: (1839.96, 2213.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12787) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (295, 1521, 5965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17305) [M::mem_pestat] mean and std.dev: (3019.39, 3276.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22975) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1021.841 CPU sec, 68.268 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1035977, 38, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (414, 841, 1238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2886) [M::mem_pestat] mean and std.dev: (707.50, 408.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (398, 1574, 2604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7016) [M::mem_pestat] mean and std.dev: (1689.77, 1483.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9222) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1008.882 CPU sec, 46.852 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036385, 33, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (350, 953, 1387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3461) [M::mem_pestat] mean and std.dev: (895.31, 671.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (291, 1654, 2915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8163) [M::mem_pestat] mean and std.dev: (1657.53, 1445.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10787) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (712, 1336, 4576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12304) [M::mem_pestat] mean and std.dev: (2608.33, 2418.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16168) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1011.487 CPU sec, 49.057 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 1035906, 26, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (290, 786, 1233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3119) [M::mem_pestat] mean and std.dev: (630.89, 440.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4062) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (197, 2312, 3599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10403) [M::mem_pestat] mean and std.dev: (2791.27, 3037.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14942) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1011.099 CPU sec, 44.611 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1035949, 20, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (341, 521, 1504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3830) [M::mem_pestat] mean and std.dev: (836.83, 826.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4993) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1533, 2854, 3881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8577) [M::mem_pestat] mean and std.dev: (2774.26, 2111.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11219) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (788, 1827, 6406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17642) [M::mem_pestat] mean and std.dev: (3302.40, 3001.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23260) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1011.225 CPU sec, 45.069 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1036552, 36, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (309, 703, 1743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4611) [M::mem_pestat] mean and std.dev: (950.75, 837.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6045) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (532, 1996, 2908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7660) [M::mem_pestat] mean and std.dev: (1706.73, 1453.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10036) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (403, 2988, 6171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17707) [M::mem_pestat] mean and std.dev: (3208.00, 3045.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23475) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1007.024 CPU sec, 44.397 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1036416, 26, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (354, 560, 1521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3855) [M::mem_pestat] mean and std.dev: (989.13, 910.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5022) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1171, 2485, 2597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5449) [M::mem_pestat] mean and std.dev: (1640.13, 1048.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6875) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (810, 1633, 2374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5502) [M::mem_pestat] mean and std.dev: (1590.00, 1093.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7066) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1025.339 CPU sec, 67.815 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1035681, 40, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 403, 2403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6625) [M::mem_pestat] mean and std.dev: (1446.38, 1815.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (621, 2059, 3720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9918) [M::mem_pestat] mean and std.dev: (2741.68, 2800.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13943) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1412, 2159, 4644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11108) [M::mem_pestat] mean and std.dev: (2756.85, 2023.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14340) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1010.976 CPU sec, 47.042 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1036828, 30, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (343, 1312, 2008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5338) [M::mem_pestat] mean and std.dev: (1151.33, 807.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7003) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1292, 2587, 3677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8447) [M::mem_pestat] mean and std.dev: (2307.44, 1757.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10832) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (685, 1703, 4904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13342) [M::mem_pestat] mean and std.dev: (2357.00, 2270.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17561) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1011.469 CPU sec, 50.088 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1036188, 28, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (458, 506, 1238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2798) [M::mem_pestat] mean and std.dev: (685.70, 372.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (654, 2587, 5722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15858) [M::mem_pestat] mean and std.dev: (3361.89, 3020.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20926) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1019, 2155, 3312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7898) [M::mem_pestat] mean and std.dev: (2245.22, 1597.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1010.092 CPU sec, 44.549 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 1036601, 29, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (400, 793, 1541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3823) [M::mem_pestat] mean and std.dev: (1159.50, 1044.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1345, 2471, 5961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15193) [M::mem_pestat] mean and std.dev: (3232.69, 2925.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19809) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (657, 1373, 1764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3978) [M::mem_pestat] mean and std.dev: (1276.10, 717.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5085) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1008.292 CPU sec, 49.457 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1037016, 38, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (319, 405, 738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1576) [M::mem_pestat] mean and std.dev: (480.29, 302.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1995) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (258, 2593, 4849) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14031) [M::mem_pestat] mean and std.dev: (3265.68, 3128.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18622) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (972, 1529, 2663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6045) [M::mem_pestat] mean and std.dev: (1758.73, 1209.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7736) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1007.264 CPU sec, 44.523 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1036180, 34, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (403, 746, 1419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3451) [M::mem_pestat] mean and std.dev: (827.29, 715.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (536, 2291, 4131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11321) [M::mem_pestat] mean and std.dev: (3013.91, 3135.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15557) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (491, 927, 5854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16580) [M::mem_pestat] mean and std.dev: (2306.58, 2425.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21943) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1031.135 CPU sec, 68.305 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 1035199, 26, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (403, 1086, 1956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5062) [M::mem_pestat] mean and std.dev: (1429.90, 1256.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.03, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (301, 2603, 4174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11920) [M::mem_pestat] mean and std.dev: (3031.35, 2857.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15793) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (753, 2473, 4399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11691) [M::mem_pestat] mean and std.dev: (2932.00, 2266.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15337) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1016.581 CPU sec, 47.268 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036139, 23, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 618, 1523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3889) [M::mem_pestat] mean and std.dev: (799.76, 758.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5072) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (129, 1226, 4797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14133) [M::mem_pestat] mean and std.dev: (2408.65, 3006.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18801) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (974, 1008, 3649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8999) [M::mem_pestat] mean and std.dev: (1604.83, 1254.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11674) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1003.238 CPU sec, 46.414 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 1036097, 28, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (373, 539, 1349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3301) [M::mem_pestat] mean and std.dev: (694.68, 505.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4277) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (226, 916, 2595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7333) [M::mem_pestat] mean and std.dev: (1219.28, 1526.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9702) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1015.499 CPU sec, 44.730 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1035613, 36, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (306, 398, 1546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4026) [M::mem_pestat] mean and std.dev: (557.64, 516.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5266) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (260, 2026, 2968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8384) [M::mem_pestat] mean and std.dev: (1783.97, 1706.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11092) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (508, 1000, 1832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4480) [M::mem_pestat] mean and std.dev: (1166.52, 795.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5804) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1008.279 CPU sec, 46.889 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036247, 44, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 570, 1768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4656) [M::mem_pestat] mean and std.dev: (977.35, 1002.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6100) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (272, 2521, 4153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11915) [M::mem_pestat] mean and std.dev: (2937.86, 2940.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15796) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1013.982 CPU sec, 44.812 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1036396, 27, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (309, 387, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2853) [M::mem_pestat] mean and std.dev: (679.19, 690.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (306, 1920, 3594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10170) [M::mem_pestat] mean and std.dev: (2899.67, 3222.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15789) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (868, 1179, 2897) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6955) [M::mem_pestat] mean and std.dev: (1329.15, 1040.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8984) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1002.262 CPU sec, 44.172 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1035724, 34, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (383, 913, 1776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4562) [M::mem_pestat] mean and std.dev: (884.84, 786.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5955) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (589, 2571, 5608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15646) [M::mem_pestat] mean and std.dev: (3301.91, 2940.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20665) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (704, 1216, 2770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6902) [M::mem_pestat] mean and std.dev: (1687.88, 1675.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8968) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1029.214 CPU sec, 67.094 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 1036861, 29, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (297, 399, 2918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8160) [M::mem_pestat] mean and std.dev: (1468.80, 1636.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (228, 1881, 2709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7671) [M::mem_pestat] mean and std.dev: (1667.37, 1871.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10152) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1011.736 CPU sec, 47.136 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 1036596, 32, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (364, 1153, 1607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4093) [M::mem_pestat] mean and std.dev: (1031.48, 861.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (677, 2585, 8478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24080) [M::mem_pestat] mean and std.dev: (3631.06, 3460.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 31881) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1449, 2676, 5704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14214) [M::mem_pestat] mean and std.dev: (3487.23, 2545.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18469) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1008.102 CPU sec, 47.360 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1037255, 29, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (442, 1172, 2057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5287) [M::mem_pestat] mean and std.dev: (1337.94, 1131.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6902) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (262, 1866, 2685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7531) [M::mem_pestat] mean and std.dev: (1649.14, 1343.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9954) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1007.011 CPU sec, 44.430 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1037134, 42, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (381, 433, 3183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8787) [M::mem_pestat] mean and std.dev: (1774.81, 2334.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.82) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (157, 1745, 3704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10798) [M::mem_pestat] mean and std.dev: (2629.52, 2875.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14345) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (733, 1107, 3169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8041) [M::mem_pestat] mean and std.dev: (1898.65, 1687.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10477) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1006.550 CPU sec, 46.066 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1037042, 36, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (356, 439, 1053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2447) [M::mem_pestat] mean and std.dev: (735.83, 540.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3144) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (869, 1915, 2598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6056) [M::mem_pestat] mean and std.dev: (1688.41, 1109.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7785) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1381, 2789, 3070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6448) [M::mem_pestat] mean and std.dev: (2348.00, 1130.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8137) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1004.514 CPU sec, 44.237 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1036038, 31, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 371, 1245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3173) [M::mem_pestat] mean and std.dev: (672.32, 598.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4137) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (250, 1577, 3569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10207) [M::mem_pestat] mean and std.dev: (2478.16, 2698.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13526) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (420, 1160, 2015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5205) [M::mem_pestat] mean and std.dev: (1269.30, 1203.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6800) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1021.060 CPU sec, 66.329 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 1036557, 26, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (336, 379, 1450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3678) [M::mem_pestat] mean and std.dev: (621.38, 695.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4792) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (280, 1206, 3015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8485) [M::mem_pestat] mean and std.dev: (1300.78, 1230.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11220) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (438, 926, 4911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13857) [M::mem_pestat] mean and std.dev: (2267.67, 2527.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18330) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1017.747 CPU sec, 47.768 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036809, 33, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (323, 422, 1293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3233) [M::mem_pestat] mean and std.dev: (711.40, 588.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4203) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (160, 1459, 8356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24748) [M::mem_pestat] mean and std.dev: (3142.48, 3513.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 32944) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1435, 1865, 2958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6004) [M::mem_pestat] mean and std.dev: (1812.06, 934.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1011.467 CPU sec, 48.324 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1036652, 29, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (356, 408, 1132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2684) [M::mem_pestat] mean and std.dev: (551.62, 325.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (321, 2586, 6332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18354) [M::mem_pestat] mean and std.dev: (3225.72, 3281.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 24365) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (866, 1367, 1813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3707) [M::mem_pestat] mean and std.dev: (1084.64, 558.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4654) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1007.414 CPU sec, 44.358 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1036462, 39, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (374, 463, 1310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3182) [M::mem_pestat] mean and std.dev: (897.88, 817.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4169) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (394, 1361, 2967) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8113) [M::mem_pestat] mean and std.dev: (1978.76, 1984.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10686) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1150, 1842, 4911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12433) [M::mem_pestat] mean and std.dev: (2594.46, 2224.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16194) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1008.963 CPU sec, 44.959 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1037353, 24, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (373, 661, 1405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3469) [M::mem_pestat] mean and std.dev: (672.11, 401.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (236, 2039, 5267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15329) [M::mem_pestat] mean and std.dev: (3052.62, 3288.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20360) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1006.430 CPU sec, 44.304 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1037064, 30, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (368, 490, 1533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3863) [M::mem_pestat] mean and std.dev: (953.72, 853.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5028) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.12, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (220, 910, 2473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6979) [M::mem_pestat] mean and std.dev: (1144.48, 1110.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9232) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (749, 1257, 2100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4802) [M::mem_pestat] mean and std.dev: (1174.20, 553.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6153) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1034.410 CPU sec, 68.305 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036165, 28, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (386, 1244, 3073) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8447) [M::mem_pestat] mean and std.dev: (1916.69, 2343.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11289) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (210, 2585, 5474) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16002) [M::mem_pestat] mean and std.dev: (2854.86, 3101.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21266) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (785, 1158, 4405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11645) [M::mem_pestat] mean and std.dev: (2555.54, 2920.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15265) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1014.863 CPU sec, 47.357 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036089, 32, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (407, 1142, 1832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4682) [M::mem_pestat] mean and std.dev: (881.24, 623.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6107) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (306, 2122, 8250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24138) [M::mem_pestat] mean and std.dev: (3178.62, 3373.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 32082) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (851, 1266, 3057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7469) [M::mem_pestat] mean and std.dev: (1478.13, 963.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9675) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1011.613 CPU sec, 50.941 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036210, 25, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (396, 943, 3827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10689) [M::mem_pestat] mean and std.dev: (1931.76, 2040.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14120) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (442, 1897, 4877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13747) [M::mem_pestat] mean and std.dev: (2878.08, 3023.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18182) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (766, 1296, 2021) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4531) [M::mem_pestat] mean and std.dev: (1401.58, 1100.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5803) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1008.469 CPU sec, 44.635 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1036275, 30, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 1374, 2126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5702) [M::mem_pestat] mean and std.dev: (1164.00, 866.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (194, 661, 2127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5993) [M::mem_pestat] mean and std.dev: (1027.43, 1272.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7926) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (429, 887, 2174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5664) [M::mem_pestat] mean and std.dev: (994.92, 865.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7409) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1020.083 CPU sec, 50.669 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036885, 23, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (435, 1999, 2571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6843) [M::mem_pestat] mean and std.dev: (1570.90, 1234.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8979) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (381, 2118, 5224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14910) [M::mem_pestat] mean and std.dev: (2945.87, 3069.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19753) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (455, 1248, 2240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5810) [M::mem_pestat] mean and std.dev: (1368.50, 1012.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7595) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1011.100 CPU sec, 44.591 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036410, 24, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (367, 418, 3072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8482) [M::mem_pestat] mean and std.dev: (1744.36, 2141.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11187) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.13, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (136, 769, 2955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8593) [M::mem_pestat] mean and std.dev: (1720.52, 2219.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11412) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (844, 1219, 2489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5779) [M::mem_pestat] mean and std.dev: (1664.50, 1296.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7424) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1052.401 CPU sec, 71.027 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 1036582, 22, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (426, 954, 1962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5034) [M::mem_pestat] mean and std.dev: (1012.16, 662.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (494, 1967, 2583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6761) [M::mem_pestat] mean and std.dev: (1662.50, 1228.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8850) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (901, 1637, 3040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7318) [M::mem_pestat] mean and std.dev: (2121.59, 1940.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9882) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1028.649 CPU sec, 48.149 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1035854, 20, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (381, 622, 1422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3504) [M::mem_pestat] mean and std.dev: (807.20, 767.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (388, 2595, 8479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24661) [M::mem_pestat] mean and std.dev: (3456.00, 3404.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 32752) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (668, 1495, 2949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7511) [M::mem_pestat] mean and std.dev: (1853.24, 1528.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9792) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1021.822 CPU sec, 51.453 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036824, 28, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (424, 1325, 3720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10312) [M::mem_pestat] mean and std.dev: (2010.25, 1966.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1394, 2559, 3570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7922) [M::mem_pestat] mean and std.dev: (2065.42, 1560.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10098) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (700, 1137, 2001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4603) [M::mem_pestat] mean and std.dev: (1404.29, 1067.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5904) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1014.931 CPU sec, 44.816 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 1036719, 29, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (332, 401, 1446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3674) [M::mem_pestat] mean and std.dev: (603.14, 554.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.04, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (708, 1807, 4138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10998) [M::mem_pestat] mean and std.dev: (2867.07, 2890.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14428) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (826, 1384, 3218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8002) [M::mem_pestat] mean and std.dev: (1860.95, 1319.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10394) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1013.880 CPU sec, 49.036 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1035972, 38, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (362, 455, 1306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3194) [M::mem_pestat] mean and std.dev: (600.76, 466.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4138) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (412, 2337, 4697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13267) [M::mem_pestat] mean and std.dev: (3131.76, 3093.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17552) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1065, 1365, 3347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7911) [M::mem_pestat] mean and std.dev: (1714.07, 1360.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10193) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1017.458 CPU sec, 44.823 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1036897, 33, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (492, 758, 2685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7071) [M::mem_pestat] mean and std.dev: (1454.53, 1419.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9264) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (458, 1926, 6929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19871) [M::mem_pestat] mean and std.dev: (3069.24, 2971.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 26342) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (778, 1353, 2310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5374) [M::mem_pestat] mean and std.dev: (1616.59, 1182.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6906) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1024.938 CPU sec, 45.222 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1035927, 43, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (379, 912, 2048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5386) [M::mem_pestat] mean and std.dev: (837.57, 673.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7055) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (111, 1317, 2966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8676) [M::mem_pestat] mean and std.dev: (2230.58, 2617.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12702) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (784, 1257, 2086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4690) [M::mem_pestat] mean and std.dev: (1469.41, 889.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5992) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1040.768 CPU sec, 70.048 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1036602, 28, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 423, 2158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5814) [M::mem_pestat] mean and std.dev: (909.07, 1113.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (360, 1387, 5799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16677) [M::mem_pestat] mean and std.dev: (2903.07, 2934.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22116) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (793, 1183, 2961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7297) [M::mem_pestat] mean and std.dev: (1722.82, 1657.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9465) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1016.590 CPU sec, 47.262 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1036782, 30, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (446, 1066, 2971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8021) [M::mem_pestat] mean and std.dev: (2039.47, 1977.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (174, 2579, 3101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8955) [M::mem_pestat] mean and std.dev: (2508.17, 2640.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13070) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (696, 1420, 2394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5790) [M::mem_pestat] mean and std.dev: (1499.71, 1247.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7488) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1014.984 CPU sec, 48.633 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1036361, 39, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (351, 979, 2131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5691) [M::mem_pestat] mean and std.dev: (1272.71, 1427.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (137, 1387, 2610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7556) [M::mem_pestat] mean and std.dev: (1546.78, 1661.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10029) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (691, 1244, 2429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5905) [M::mem_pestat] mean and std.dev: (1484.94, 1038.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7643) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1012.483 CPU sec, 44.779 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1036187, 26, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (373, 486, 1490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3724) [M::mem_pestat] mean and std.dev: (778.95, 715.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4841) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.04, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (235, 2013, 5182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15076) [M::mem_pestat] mean and std.dev: (2983.73, 2932.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20023) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (576, 933, 7573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 21567) [M::mem_pestat] mean and std.dev: (2547.92, 3218.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 28564) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1013.466 CPU sec, 49.956 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1036677, 32, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (402, 956, 1640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4116) [M::mem_pestat] mean and std.dev: (999.05, 908.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (765, 2479, 3188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8034) [M::mem_pestat] mean and std.dev: (2003.13, 1463.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10457) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1011.576 CPU sec, 44.662 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1036853, 37, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (391, 1103, 1915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4963) [M::mem_pestat] mean and std.dev: (1178.94, 1158.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (400, 1846, 3654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10162) [M::mem_pestat] mean and std.dev: (2546.49, 2774.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13646) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1103, 1438, 2541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5417) [M::mem_pestat] mean and std.dev: (1463.65, 854.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6855) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1052.905 CPU sec, 70.906 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1035498, 32, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (439, 1071, 2362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6208) [M::mem_pestat] mean and std.dev: (1328.89, 1038.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8131) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (480, 2464, 4289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11907) [M::mem_pestat] mean and std.dev: (2877.03, 2677.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15716) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (684, 1328, 5963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16521) [M::mem_pestat] mean and std.dev: (2769.00, 2505.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21800) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1023.884 CPU sec, 47.919 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1036201, 40, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (389, 722, 2439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6539) [M::mem_pestat] mean and std.dev: (1318.24, 1564.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (205, 1448, 2998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8584) [M::mem_pestat] mean and std.dev: (2368.65, 2737.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13318) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1237, 1821, 7448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19870) [M::mem_pestat] mean and std.dev: (3131.08, 2886.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 26081) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1021.047 CPU sec, 49.934 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036150, 24, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (387, 1063, 1853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4785) [M::mem_pestat] mean and std.dev: (1159.00, 1047.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6251) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (92, 2226, 2861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8399) [M::mem_pestat] mean and std.dev: (1904.04, 1936.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11168) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1036, 1562, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5749) [M::mem_pestat] mean and std.dev: (1747.56, 1011.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7320) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1016.811 CPU sec, 44.851 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1037018, 30, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (294, 409, 817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1863) [M::mem_pestat] mean and std.dev: (425.25, 263.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2386) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (419, 2531, 3817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10613) [M::mem_pestat] mean and std.dev: (2895.97, 2750.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14011) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (556, 1477, 2216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5536) [M::mem_pestat] mean and std.dev: (1437.60, 917.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7196) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1024.512 CPU sec, 51.808 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036085, 32, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (369, 810, 1238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2976) [M::mem_pestat] mean and std.dev: (810.90, 624.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (188, 1018, 2367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6725) [M::mem_pestat] mean and std.dev: (1255.73, 1446.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8904) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1238, 2460, 4138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9938) [M::mem_pestat] mean and std.dev: (3082.70, 2619.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13560) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1014.773 CPU sec, 44.683 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1036314, 28, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (380, 592, 3210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8870) [M::mem_pestat] mean and std.dev: (1929.62, 2622.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (117, 1367, 2562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7452) [M::mem_pestat] mean and std.dev: (1264.28, 1346.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9897) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (839, 1374, 2111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4655) [M::mem_pestat] mean and std.dev: (1407.58, 984.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5927) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1043.480 CPU sec, 70.705 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1035694, 34, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (319, 414, 3233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9061) [M::mem_pestat] mean and std.dev: (1510.53, 2231.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11975) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (247, 1072, 1878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5140) [M::mem_pestat] mean and std.dev: (912.34, 856.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6771) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (993, 1107, 1637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2925) [M::mem_pestat] mean and std.dev: (1194.36, 575.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3569) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1026.607 CPU sec, 48.253 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036796, 40, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (360, 397, 986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2238) [M::mem_pestat] mean and std.dev: (575.96, 465.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.12, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (644, 2579, 5125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14087) [M::mem_pestat] mean and std.dev: (3137.35, 2840.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18568) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (979, 1523, 1823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3511) [M::mem_pestat] mean and std.dev: (1385.70, 728.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4355) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1018.819 CPU sec, 48.770 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1036896, 28, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (394, 986, 2164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5704) [M::mem_pestat] mean and std.dev: (1216.88, 1191.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (255, 702, 2595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7275) [M::mem_pestat] mean and std.dev: (1341.38, 1832.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9615) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (908, 1216, 1889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3851) [M::mem_pestat] mean and std.dev: (1057.53, 482.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4832) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1011.872 CPU sec, 44.625 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036394, 36, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (396, 498, 2505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6723) [M::mem_pestat] mean and std.dev: (1451.10, 1738.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (303, 1443, 2613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7233) [M::mem_pestat] mean and std.dev: (1484.52, 1324.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9543) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (662, 1471, 2367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5777) [M::mem_pestat] mean and std.dev: (1589.70, 1183.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7482) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1022.950 CPU sec, 51.006 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1035781, 43, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (344, 668, 1270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3122) [M::mem_pestat] mean and std.dev: (779.15, 626.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4048) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (256, 1973, 2925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8263) [M::mem_pestat] mean and std.dev: (2027.52, 2019.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10932) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1016.473 CPU sec, 44.951 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1036000, 37, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (313, 929, 1495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3859) [M::mem_pestat] mean and std.dev: (747.53, 676.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5041) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1223, 2178, 3161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7037) [M::mem_pestat] mean and std.dev: (1960.16, 1623.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8975) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (470, 1156, 2067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5261) [M::mem_pestat] mean and std.dev: (1191.85, 770.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6858) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1036.750 CPU sec, 69.432 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1037237, 24, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (373, 412, 4815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13699) [M::mem_pestat] mean and std.dev: (2095.05, 2836.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18141) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (298, 2586, 4094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11686) [M::mem_pestat] mean and std.dev: (2888.21, 2910.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15482) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (432, 1589, 2014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5178) [M::mem_pestat] mean and std.dev: (1472.86, 993.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6760) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1015.909 CPU sec, 47.727 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 1035751, 38, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (393, 1341, 1870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4824) [M::mem_pestat] mean and std.dev: (1270.96, 907.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1264, 2586, 3594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8254) [M::mem_pestat] mean and std.dev: (2014.16, 1417.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10584) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (777, 1163, 2135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4851) [M::mem_pestat] mean and std.dev: (1294.04, 797.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6209) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1019.716 CPU sec, 50.556 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036581, 43, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (369, 455, 834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1764) [M::mem_pestat] mean and std.dev: (631.13, 363.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2229) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (212, 1860, 3747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10817) [M::mem_pestat] mean and std.dev: (2551.65, 2803.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14352) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (681, 1751, 4272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11454) [M::mem_pestat] mean and std.dev: (2396.50, 2053.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15045) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1016.927 CPU sec, 44.880 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1035727, 36, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (344, 457, 1248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3056) [M::mem_pestat] mean and std.dev: (627.39, 539.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3960) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (418, 1751, 2854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7726) [M::mem_pestat] mean and std.dev: (1741.03, 1763.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10162) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (865, 1222, 2046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4408) [M::mem_pestat] mean and std.dev: (1358.24, 1006.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5589) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1034.458 CPU sec, 54.287 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 1035766, 41, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (370, 877, 2313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6199) [M::mem_pestat] mean and std.dev: (920.28, 789.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8142) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.13, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (299, 1449, 4608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13226) [M::mem_pestat] mean and std.dev: (2469.10, 2720.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17535) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1032, 1932, 3211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7569) [M::mem_pestat] mean and std.dev: (1764.00, 1219.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9748) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1034.117 CPU sec, 45.633 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1036331, 20, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (406, 1003, 2406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6406) [M::mem_pestat] mean and std.dev: (1279.43, 1217.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (247, 535, 3420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9766) [M::mem_pestat] mean and std.dev: (2166.95, 2931.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13895) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (528, 1365, 2712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7080) [M::mem_pestat] mean and std.dev: (1319.32, 897.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9264) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1026.064 CPU sec, 45.283 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1037057, 40, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 359, 978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2280) [M::mem_pestat] mean and std.dev: (606.39, 543.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2931) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (377, 1458, 2881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7889) [M::mem_pestat] mean and std.dev: (1459.63, 1270.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10393) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (553, 1253, 5306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14812) [M::mem_pestat] mean and std.dev: (2771.33, 2768.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19565) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1054.884 CPU sec, 68.941 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 1036778, 28, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (328, 563, 1154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2806) [M::mem_pestat] mean and std.dev: (774.42, 631.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (651, 1970, 4789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13065) [M::mem_pestat] mean and std.dev: (2833.61, 2794.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17203) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1014, 1883, 4087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10233) [M::mem_pestat] mean and std.dev: (2508.55, 2308.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13306) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1029.685 CPU sec, 48.497 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036633, 38, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (390, 772, 1740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4440) [M::mem_pestat] mean and std.dev: (911.53, 653.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (276, 1710, 2591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7221) [M::mem_pestat] mean and std.dev: (1505.83, 1127.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9536) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1133, 1870, 5775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15059) [M::mem_pestat] mean and std.dev: (3410.44, 3399.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19701) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1019.473 CPU sec, 50.860 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1036041, 32, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (372, 641, 1755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4521) [M::mem_pestat] mean and std.dev: (647.60, 471.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5904) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (379, 1497, 2598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7036) [M::mem_pestat] mean and std.dev: (1478.48, 1312.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9255) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (440, 1962, 4521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12683) [M::mem_pestat] mean and std.dev: (2698.21, 2231.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16764) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1016.244 CPU sec, 44.783 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036043, 37, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (361, 454, 1499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3775) [M::mem_pestat] mean and std.dev: (849.50, 943.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4913) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (212, 1494, 7331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 21569) [M::mem_pestat] mean and std.dev: (2755.97, 3167.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 28688) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (851, 1546, 2503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5807) [M::mem_pestat] mean and std.dev: (1777.29, 1478.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7691) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1018.787 CPU sec, 50.985 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1036940, 18, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (360, 716, 1813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4719) [M::mem_pestat] mean and std.dev: (979.52, 861.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6172) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.04, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (361, 2559, 4814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13720) [M::mem_pestat] mean and std.dev: (2947.67, 3136.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18173) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (976, 2435, 4672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12064) [M::mem_pestat] mean and std.dev: (3079.50, 2446.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15760) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1017.514 CPU sec, 44.990 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 1036488, 29, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (278, 898, 5585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16199) [M::mem_pestat] mean and std.dev: (2597.40, 2806.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (310, 1876, 2796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7768) [M::mem_pestat] mean and std.dev: (1661.41, 1253.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10254) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (743, 2069, 5012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13550) [M::mem_pestat] mean and std.dev: (2773.23, 2608.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17819) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1043.139 CPU sec, 67.917 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 1036317, 33, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (362, 673, 981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2219) [M::mem_pestat] mean and std.dev: (625.54, 379.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2838) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (200, 783, 2837) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8111) [M::mem_pestat] mean and std.dev: (1432.13, 1542.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10748) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (694, 1094, 2314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5554) [M::mem_pestat] mean and std.dev: (1385.06, 1277.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7174) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1021.550 CPU sec, 47.677 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 1036578, 22, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (354, 379, 901) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1995) [M::mem_pestat] mean and std.dev: (519.69, 468.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.12, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1881, 2584, 3211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5871) [M::mem_pestat] mean and std.dev: (2307.05, 1061.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7201) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (833, 1146, 3167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7835) [M::mem_pestat] mean and std.dev: (1271.20, 853.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10169) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1020.321 CPU sec, 45.810 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036897, 33, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (354, 752, 1650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4242) [M::mem_pestat] mean and std.dev: (924.33, 781.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (595, 1862, 3412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9046) [M::mem_pestat] mean and std.dev: (2552.18, 2741.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13517) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (904, 1676, 2145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4627) [M::mem_pestat] mean and std.dev: (1343.22, 849.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5868) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1019.032 CPU sec, 44.922 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1036212, 26, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (383, 714, 1112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2570) [M::mem_pestat] mean and std.dev: (689.29, 449.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3299) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (293, 1885, 2611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7247) [M::mem_pestat] mean and std.dev: (1338.70, 1200.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9565) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (772, 1320, 2555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6121) [M::mem_pestat] mean and std.dev: (1799.27, 1443.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7904) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1016.233 CPU sec, 45.442 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1036622, 29, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (373, 749, 1336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3262) [M::mem_pestat] mean and std.dev: (700.50, 462.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4225) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (293, 1909, 4299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12311) [M::mem_pestat] mean and std.dev: (2742.52, 2786.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16317) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1003, 1875, 3956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9862) [M::mem_pestat] mean and std.dev: (2567.50, 2221.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12815) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1016.193 CPU sec, 44.791 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036176, 35, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (385, 998, 1635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4135) [M::mem_pestat] mean and std.dev: (972.16, 877.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (427, 1844, 7370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 21256) [M::mem_pestat] mean and std.dev: (2995.80, 3290.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 28199) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1004, 1245, 3503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8501) [M::mem_pestat] mean and std.dev: (2429.80, 2306.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11655) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1038.125 CPU sec, 67.721 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 1035529, 36, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (394, 708, 1453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3571) [M::mem_pestat] mean and std.dev: (710.30, 555.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (844, 2512, 3163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7801) [M::mem_pestat] mean and std.dev: (1993.41, 1444.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10120) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (898, 1218, 1540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2824) [M::mem_pestat] mean and std.dev: (1142.67, 566.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3466) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1020.263 CPU sec, 47.846 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1035738, 35, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (410, 1032, 1611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4013) [M::mem_pestat] mean and std.dev: (1059.26, 783.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5214) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (299, 2265, 3796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10790) [M::mem_pestat] mean and std.dev: (2707.51, 2836.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14287) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1426, 1977, 2257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3919) [M::mem_pestat] mean and std.dev: (1744.58, 573.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4750) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1016.684 CPU sec, 50.776 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1036218, 34, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (401, 1767, 4302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12104) [M::mem_pestat] mean and std.dev: (2213.56, 2139.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16005) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (978, 2485, 3183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7593) [M::mem_pestat] mean and std.dev: (1983.35, 1459.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9798) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (870, 1060, 4855) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12825) [M::mem_pestat] mean and std.dev: (2424.21, 2256.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16810) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1017.758 CPU sec, 44.979 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1035735, 23, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (347, 436, 1014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2348) [M::mem_pestat] mean and std.dev: (625.47, 515.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3015) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1127, 1998, 4142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10172) [M::mem_pestat] mean and std.dev: (2785.91, 2562.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13187) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (366, 929, 2231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5961) [M::mem_pestat] mean and std.dev: (1282.54, 1400.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1015.181 CPU sec, 50.664 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036515, 27, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (417, 1389, 3013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8205) [M::mem_pestat] mean and std.dev: (1943.38, 2099.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1752, 2599, 4271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9309) [M::mem_pestat] mean and std.dev: (3157.42, 2731.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14083) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (632, 1032, 3237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8447) [M::mem_pestat] mean and std.dev: (1467.67, 1239.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11052) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1016.096 CPU sec, 44.835 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 1036185, 27, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 394, 1791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4697) [M::mem_pestat] mean and std.dev: (523.56, 486.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6150) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (895, 2140, 2591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5983) [M::mem_pestat] mean and std.dev: (1591.75, 1101.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7679) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1004, 1692, 2279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4829) [M::mem_pestat] mean and std.dev: (1575.22, 905.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6104) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1036.183 CPU sec, 69.537 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1035417, 41, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (391, 638, 1448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3562) [M::mem_pestat] mean and std.dev: (811.59, 582.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (174, 1562, 2586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7410) [M::mem_pestat] mean and std.dev: (1474.42, 1664.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9822) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (537, 940, 1597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3717) [M::mem_pestat] mean and std.dev: (877.93, 469.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4777) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1021.362 CPU sec, 47.315 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036793, 24, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (399, 905, 2765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7497) [M::mem_pestat] mean and std.dev: (1821.29, 2150.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (683, 2102, 2852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7190) [M::mem_pestat] mean and std.dev: (1773.24, 1585.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9359) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (883, 1376, 2729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6421) [M::mem_pestat] mean and std.dev: (1601.05, 939.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8267) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1017.896 CPU sec, 50.764 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036170, 34, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 397, 1785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4723) [M::mem_pestat] mean and std.dev: (928.12, 1085.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6192) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (404, 2148, 4018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11246) [M::mem_pestat] mean and std.dev: (2820.09, 2725.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14860) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (920, 1445, 2058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4334) [M::mem_pestat] mean and std.dev: (1183.60, 417.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5472) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1012.140 CPU sec, 44.596 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1037231, 29, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (415, 884, 1368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3274) [M::mem_pestat] mean and std.dev: (875.56, 595.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4227) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (201, 2556, 5789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16965) [M::mem_pestat] mean and std.dev: (2913.55, 2944.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22553) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (561, 1414, 2789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7245) [M::mem_pestat] mean and std.dev: (1817.06, 1598.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9473) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1014.214 CPU sec, 47.569 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1036438, 33, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 751, 1721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4615) [M::mem_pestat] mean and std.dev: (759.79, 648.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6062) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (244, 1234, 2592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7288) [M::mem_pestat] mean and std.dev: (1369.60, 1309.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9636) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (812, 1792, 3376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8504) [M::mem_pestat] mean and std.dev: (2192.76, 1933.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11068) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1015.413 CPU sec, 44.920 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036491, 26, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (306, 427, 3923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11157) [M::mem_pestat] mean and std.dev: (2288.91, 3195.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15069) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (504, 1882, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6807) [M::mem_pestat] mean and std.dev: (1761.20, 1446.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8908) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (898, 1379, 1792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3580) [M::mem_pestat] mean and std.dev: (1277.38, 676.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4474) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1013.033 CPU sec, 44.714 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1037050, 26, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 410, 992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2582) [M::mem_pestat] mean and std.dev: (532.94, 559.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.05, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (385, 2593, 3439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9547) [M::mem_pestat] mean and std.dev: (3005.46, 2742.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13974) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (429, 1037, 2089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5409) [M::mem_pestat] mean and std.dev: (1215.38, 1049.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7069) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1030.905 CPU sec, 68.795 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036255, 41, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (374, 1011, 1834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4754) [M::mem_pestat] mean and std.dev: (1073.30, 877.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6214) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (492, 2540, 4567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12717) [M::mem_pestat] mean and std.dev: (2981.95, 2827.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16792) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1026, 1567, 2494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5430) [M::mem_pestat] mean and std.dev: (1795.21, 1326.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7099) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1018.194 CPU sec, 47.455 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1037544, 34, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (413, 782, 1991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5147) [M::mem_pestat] mean and std.dev: (1276.43, 1306.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.12, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (398, 1999, 3525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9779) [M::mem_pestat] mean and std.dev: (2437.48, 2631.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12965) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1004.452 CPU sec, 48.961 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 1036209, 20, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (443, 751, 3282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8960) [M::mem_pestat] mean and std.dev: (1875.73, 2002.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1379, 2592, 6373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16361) [M::mem_pestat] mean and std.dev: (3583.30, 2973.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21355) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1350, 2057, 2411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4533) [M::mem_pestat] mean and std.dev: (1728.18, 693.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5594) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1008.034 CPU sec, 44.526 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1037170, 30, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (321, 400, 919) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2115) [M::mem_pestat] mean and std.dev: (602.75, 489.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (750, 2483, 2829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6987) [M::mem_pestat] mean and std.dev: (2203.72, 1713.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9066) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (475, 1117, 1642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3976) [M::mem_pestat] mean and std.dev: (1259.79, 842.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5143) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1016.828 CPU sec, 50.731 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1036158, 22, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (415, 866, 1761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4453) [M::mem_pestat] mean and std.dev: (958.63, 669.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (254, 2512, 2599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7289) [M::mem_pestat] mean and std.dev: (1823.24, 1418.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9634) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (730, 1477, 2257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5311) [M::mem_pestat] mean and std.dev: (1737.27, 1368.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7212) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1015.385 CPU sec, 44.775 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 1036481, 25, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 440, 688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1466) [M::mem_pestat] mean and std.dev: (436.75, 275.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (407, 1854, 3870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10796) [M::mem_pestat] mean and std.dev: (2557.16, 2681.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14259) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (540, 873, 1494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3402) [M::mem_pestat] mean and std.dev: (797.50, 338.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4356) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1026.295 CPU sec, 66.740 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036404, 33, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (368, 994, 2983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8213) [M::mem_pestat] mean and std.dev: (1627.30, 1851.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10828) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (377, 2565, 8473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24665) [M::mem_pestat] mean and std.dev: (3709.58, 3458.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 32761) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (613, 953, 1709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3901) [M::mem_pestat] mean and std.dev: (1075.60, 720.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4997) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1016.536 CPU sec, 47.349 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036090, 34, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (373, 1209, 2078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5488) [M::mem_pestat] mean and std.dev: (1484.59, 1394.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7193) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (616, 2564, 3558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9442) [M::mem_pestat] mean and std.dev: (2548.88, 2189.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12384) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (734, 1076, 1797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3923) [M::mem_pestat] mean and std.dev: (1201.56, 815.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4986) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1005.713 CPU sec, 49.816 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1036237, 32, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (373, 599, 1559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3931) [M::mem_pestat] mean and std.dev: (830.10, 622.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5117) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (325, 1940, 3763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10639) [M::mem_pestat] mean and std.dev: (2502.72, 2717.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14077) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1151, 1582, 3623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8567) [M::mem_pestat] mean and std.dev: (2156.57, 1543.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11039) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1009.716 CPU sec, 44.510 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036526, 26, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (384, 1038, 2276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6060) [M::mem_pestat] mean and std.dev: (1375.40, 1468.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (259, 2066, 2604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7294) [M::mem_pestat] mean and std.dev: (1528.30, 1383.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9639) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1009.504 CPU sec, 48.066 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1036584, 29, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (300, 370, 865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1995) [M::mem_pestat] mean and std.dev: (320.54, 201.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (250, 2590, 4151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11953) [M::mem_pestat] mean and std.dev: (2920.45, 2692.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15854) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (419, 1041, 2000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5162) [M::mem_pestat] mean and std.dev: (1128.47, 836.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6743) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1008.475 CPU sec, 44.421 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1036620, 19, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (425, 994, 1883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4799) [M::mem_pestat] mean and std.dev: (1150.60, 903.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6257) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (375, 1739, 2582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6996) [M::mem_pestat] mean and std.dev: (1682.72, 1376.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9203) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1042, 1314, 2744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6148) [M::mem_pestat] mean and std.dev: (1635.00, 997.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7850) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1024.896 CPU sec, 68.793 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 1035803, 35, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (410, 863, 1681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4223) [M::mem_pestat] mean and std.dev: (940.64, 630.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (299, 2579, 5415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15647) [M::mem_pestat] mean and std.dev: (3197.60, 3006.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20763) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (862, 2253, 4722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12442) [M::mem_pestat] mean and std.dev: (2891.12, 2516.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16302) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1013.700 CPU sec, 47.145 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1037502, 32, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (348, 532, 1033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2403) [M::mem_pestat] mean and std.dev: (698.92, 584.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3088) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (631, 2463, 3870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10348) [M::mem_pestat] mean and std.dev: (2702.00, 2680.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13587) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1197, 1433, 2977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6537) [M::mem_pestat] mean and std.dev: (1760.19, 1266.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1012.594 CPU sec, 49.132 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 1036884, 31, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (392, 991, 1629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4103) [M::mem_pestat] mean and std.dev: (946.18, 876.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5340) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (332, 1869, 3802) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10742) [M::mem_pestat] mean and std.dev: (2850.13, 3020.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14930) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (611, 1469, 2669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6785) [M::mem_pestat] mean and std.dev: (1696.00, 1328.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8843) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1014.822 CPU sec, 44.866 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1036011, 33, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (364, 394, 1838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4786) [M::mem_pestat] mean and std.dev: (910.24, 1038.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6260) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (515, 1455, 3602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9776) [M::mem_pestat] mean and std.dev: (2153.15, 2086.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12863) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (845, 1402, 2451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5663) [M::mem_pestat] mean and std.dev: (1521.62, 1000.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7269) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1016.356 CPU sec, 49.164 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1036195, 34, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (364, 663, 1756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4540) [M::mem_pestat] mean and std.dev: (953.11, 993.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5932) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (624, 2596, 7357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 20823) [M::mem_pestat] mean and std.dev: (3619.82, 3300.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 27556) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (665, 1309, 4153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11129) [M::mem_pestat] mean and std.dev: (2495.50, 2403.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14617) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1012.753 CPU sec, 44.734 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1035992, 42, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (349, 429, 1692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4378) [M::mem_pestat] mean and std.dev: (1060.22, 1194.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5839) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (231, 1312, 2588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7302) [M::mem_pestat] mean and std.dev: (1566.08, 1718.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9659) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (952, 1037, 1278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (300, 1930) [M::mem_pestat] mean and std.dev: (943.15, 298.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2256) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1035.869 CPU sec, 68.461 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1036210, 25, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (366, 895, 2149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5715) [M::mem_pestat] mean and std.dev: (1191.33, 1018.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (672, 1909, 3292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8532) [M::mem_pestat] mean and std.dev: (2193.00, 1955.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11152) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1084, 1614, 1747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3073) [M::mem_pestat] mean and std.dev: (1357.43, 478.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3736) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1020.937 CPU sec, 47.748 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 1036862, 41, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (382, 1564, 3968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11140) [M::mem_pestat] mean and std.dev: (2281.93, 2168.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.12, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (568, 1961, 4870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13474) [M::mem_pestat] mean and std.dev: (2908.39, 2835.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17776) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1011.002 CPU sec, 49.246 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1036504, 36, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (326, 1008, 1739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4565) [M::mem_pestat] mean and std.dev: (1070.14, 895.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5978) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (2501, 2905, 7373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17117) [M::mem_pestat] mean and std.dev: (4038.47, 2948.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21989) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (851, 1642, 3607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9119) [M::mem_pestat] mean and std.dev: (2165.54, 2025.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11875) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1014.889 CPU sec, 44.750 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1036112, 26, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (364, 633, 1886) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4930) [M::mem_pestat] mean and std.dev: (1091.18, 1080.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6452) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (441, 1447, 2597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6909) [M::mem_pestat] mean and std.dev: (1495.62, 1163.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9065) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (479, 886, 1259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2819) [M::mem_pestat] mean and std.dev: (777.10, 419.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3599) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1024.800 CPU sec, 51.684 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 1036608, 24, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (419, 1349, 2592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6938) [M::mem_pestat] mean and std.dev: (1796.27, 1784.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9111) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (138, 1373, 2616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7572) [M::mem_pestat] mean and std.dev: (1240.33, 1361.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10050) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (644, 1486, 2162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5198) [M::mem_pestat] mean and std.dev: (1592.29, 1215.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6716) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1016.505 CPU sec, 44.858 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1035504, 31, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (409, 1646, 2403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6391) [M::mem_pestat] mean and std.dev: (1674.06, 1615.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8385) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (391, 2579, 3041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8341) [M::mem_pestat] mean and std.dev: (1870.86, 1494.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10991) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1188, 2527, 4812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12060) [M::mem_pestat] mean and std.dev: (3111.89, 2252.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15684) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1020.737 CPU sec, 45.309 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036935, 30, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (381, 919, 1449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3585) [M::mem_pestat] mean and std.dev: (894.23, 710.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (2565, 2942, 5914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12612) [M::mem_pestat] mean and std.dev: (3732.63, 2658.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15961) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (899, 2062, 3488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8666) [M::mem_pestat] mean and std.dev: (2176.93, 1823.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11255) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1039.876 CPU sec, 70.810 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036220, 17, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (344, 390, 1574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4034) [M::mem_pestat] mean and std.dev: (946.76, 897.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5264) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.12, 17.88) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (383, 1401, 4722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13400) [M::mem_pestat] mean and std.dev: (2544.06, 2611.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17739) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (761, 1388, 3531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9071) [M::mem_pestat] mean and std.dev: (1957.60, 2032.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11841) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1019.976 CPU sec, 47.773 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1036101, 36, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (373, 660, 1720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4414) [M::mem_pestat] mean and std.dev: (1043.00, 892.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (250, 2572, 7378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 21634) [M::mem_pestat] mean and std.dev: (3198.56, 3190.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 28762) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (727, 1024, 1433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2845) [M::mem_pestat] mean and std.dev: (1057.54, 386.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3551) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1011.995 CPU sec, 49.467 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1036162, 24, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (409, 625, 1361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3265) [M::mem_pestat] mean and std.dev: (729.38, 631.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4217) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (295, 1725, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7219) [M::mem_pestat] mean and std.dev: (1471.91, 1131.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9527) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (798, 1663, 2747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6645) [M::mem_pestat] mean and std.dev: (1770.90, 1457.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8594) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1015.387 CPU sec, 44.785 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036581, 36, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (424, 900, 2070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5362) [M::mem_pestat] mean and std.dev: (912.95, 809.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7008) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (250, 1246, 2842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8026) [M::mem_pestat] mean and std.dev: (1482.18, 1374.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10618) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (678, 1433, 2446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5982) [M::mem_pestat] mean and std.dev: (1703.18, 1693.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8478) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1013.486 CPU sec, 49.270 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1036760, 37, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (467, 1424, 2053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5225) [M::mem_pestat] mean and std.dev: (1353.38, 1065.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (640, 2053, 5701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15823) [M::mem_pestat] mean and std.dev: (3085.76, 3097.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20884) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1297, 1807, 3312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7342) [M::mem_pestat] mean and std.dev: (1876.21, 1099.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9357) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1012.652 CPU sec, 44.773 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1036928, 25, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (319, 783, 1729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4549) [M::mem_pestat] mean and std.dev: (924.75, 785.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5959) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1080, 2610, 8508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 23364) [M::mem_pestat] mean and std.dev: (3808.76, 3331.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 30792) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (627, 1316, 2147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5187) [M::mem_pestat] mean and std.dev: (1355.18, 878.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6707) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1042.838 CPU sec, 68.838 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1036692, 29, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (444, 1210, 2072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5328) [M::mem_pestat] mean and std.dev: (1193.45, 759.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6956) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (145, 1520, 6697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19801) [M::mem_pestat] mean and std.dev: (2912.76, 3231.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 26353) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1040, 1710, 3494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8402) [M::mem_pestat] mean and std.dev: (1968.92, 1426.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10856) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1018.652 CPU sec, 47.449 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1036019, 37, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (405, 728, 1599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3987) [M::mem_pestat] mean and std.dev: (925.32, 841.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5181) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (502, 2519, 7865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22591) [M::mem_pestat] mean and std.dev: (3438.11, 3260.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 29954) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (819, 1041, 2391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5535) [M::mem_pestat] mean and std.dev: (1117.43, 708.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7107) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1022.437 CPU sec, 51.826 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 1035926, 27, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (375, 664, 1417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3501) [M::mem_pestat] mean and std.dev: (872.00, 712.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.88) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1107, 2470, 4804) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12198) [M::mem_pestat] mean and std.dev: (3241.22, 3058.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15895) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1313, 2101, 3402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7580) [M::mem_pestat] mean and std.dev: (2316.12, 1614.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9669) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1014.856 CPU sec, 44.762 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1036785, 28, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (345, 411, 1442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3636) [M::mem_pestat] mean and std.dev: (798.00, 762.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (213, 1552, 3854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11136) [M::mem_pestat] mean and std.dev: (2649.71, 2884.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14777) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1019.620 CPU sec, 50.292 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1036244, 30, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (384, 916, 2907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7953) [M::mem_pestat] mean and std.dev: (1722.29, 2013.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (795, 2583, 4831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12903) [M::mem_pestat] mean and std.dev: (3310.80, 3037.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16939) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (499, 765, 1921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4765) [M::mem_pestat] mean and std.dev: (1015.07, 798.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6187) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1013.357 CPU sec, 44.705 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1037286, 42, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (361, 1257, 4437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12589) [M::mem_pestat] mean and std.dev: (2312.71, 2274.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16665) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (510, 2595, 4723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13149) [M::mem_pestat] mean and std.dev: (3101.07, 2935.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17362) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (411, 1154, 3199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8775) [M::mem_pestat] mean and std.dev: (1661.38, 1355.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11563) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1047.079 CPU sec, 70.843 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036067, 22, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (296, 406, 1032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2504) [M::mem_pestat] mean and std.dev: (443.14, 264.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3240) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.13, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (477, 1953, 2595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6831) [M::mem_pestat] mean and std.dev: (1459.06, 1332.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8949) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (766, 913, 2286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5326) [M::mem_pestat] mean and std.dev: (1481.36, 1372.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6973) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1032.972 CPU sec, 48.649 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 1036966, 38, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (359, 502, 3088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8546) [M::mem_pestat] mean and std.dev: (1586.50, 1849.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11275) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (178, 768, 2851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8197) [M::mem_pestat] mean and std.dev: (1369.83, 1396.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10870) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (759, 1327, 3227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8163) [M::mem_pestat] mean and std.dev: (1919.00, 1551.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10631) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1024.670 CPU sec, 52.813 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 1036984, 35, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (376, 391, 2483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6697) [M::mem_pestat] mean and std.dev: (928.23, 1326.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (235, 2590, 3583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10279) [M::mem_pestat] mean and std.dev: (2595.83, 2479.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13627) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1013.049 CPU sec, 44.815 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 1036969, 29, 16) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (501, 1549, 4108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11322) [M::mem_pestat] mean and std.dev: (2740.66, 2970.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14929) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (898, 1167, 2230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4894) [M::mem_pestat] mean and std.dev: (1233.57, 818.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6226) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1026.756 CPU sec, 50.952 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 1036620, 31, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (586, 1229, 1700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3928) [M::mem_pestat] mean and std.dev: (1119.27, 691.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5042) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1249, 2118, 4835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12007) [M::mem_pestat] mean and std.dev: (3180.32, 2872.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15593) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (908, 1225, 4354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11246) [M::mem_pestat] mean and std.dev: (2508.12, 2297.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14692) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1014.585 CPU sec, 44.714 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1036216, 23, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 436, 1859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4897) [M::mem_pestat] mean and std.dev: (948.10, 953.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (884, 2575, 2884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6884) [M::mem_pestat] mean and std.dev: (1799.25, 1343.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8884) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (719, 1054, 1917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4313) [M::mem_pestat] mean and std.dev: (1291.39, 1071.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5578) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1024.087 CPU sec, 67.173 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036076, 31, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (371, 515, 2869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7865) [M::mem_pestat] mean and std.dev: (1344.35, 1407.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1957, 2602, 7398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18280) [M::mem_pestat] mean and std.dev: (3931.10, 3070.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23721) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (962, 1897, 4940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12896) [M::mem_pestat] mean and std.dev: (2836.77, 1911.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16874) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1012.487 CPU sec, 46.986 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036215, 25, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (423, 1004, 3961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11037) [M::mem_pestat] mean and std.dev: (2045.65, 2352.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (299, 1596, 2592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7178) [M::mem_pestat] mean and std.dev: (1748.04, 1529.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9471) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (376, 659, 1131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2641) [M::mem_pestat] mean and std.dev: (717.77, 594.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3396) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1017.869 CPU sec, 51.678 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1036016, 39, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (414, 785, 1753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4431) [M::mem_pestat] mean and std.dev: (1093.94, 826.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (405, 2563, 3592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9966) [M::mem_pestat] mean and std.dev: (2699.54, 2572.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13153) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (839, 1381, 2962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7208) [M::mem_pestat] mean and std.dev: (1887.71, 1722.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9331) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1012.347 CPU sec, 44.722 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1036610, 29, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (420, 651, 2467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6561) [M::mem_pestat] mean and std.dev: (1374.92, 1622.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1264, 2569, 4659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11449) [M::mem_pestat] mean and std.dev: (3213.34, 2857.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14844) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (548, 1440, 2168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5408) [M::mem_pestat] mean and std.dev: (1326.31, 966.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7028) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1013.492 CPU sec, 48.948 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036846, 30, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (556, 854, 1641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3811) [M::mem_pestat] mean and std.dev: (1030.87, 743.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (117, 798, 3439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10083) [M::mem_pestat] mean and std.dev: (2316.40, 2703.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13405) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (743, 1145, 1628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3398) [M::mem_pestat] mean and std.dev: (1133.71, 652.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4283) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1008.493 CPU sec, 44.394 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1036538, 34, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1002, 1459, 3780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9336) [M::mem_pestat] mean and std.dev: (2286.80, 1970.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12114) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.12, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (487, 2311, 3666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10024) [M::mem_pestat] mean and std.dev: (2698.35, 2689.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13455) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (596, 780, 1640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3728) [M::mem_pestat] mean and std.dev: (984.09, 566.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4772) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1012.852 CPU sec, 44.855 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 1036822, 32, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (388, 1160, 1951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5077) [M::mem_pestat] mean and std.dev: (1013.81, 758.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.06, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (185, 1241, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7439) [M::mem_pestat] mean and std.dev: (1256.10, 1167.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9857) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (700, 1282, 2912) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7336) [M::mem_pestat] mean and std.dev: (1891.86, 1735.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9548) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1026.174 CPU sec, 66.644 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1036110, 40, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (357, 727, 1438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3600) [M::mem_pestat] mean and std.dev: (804.61, 636.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (663, 2277, 3726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9852) [M::mem_pestat] mean and std.dev: (3051.15, 2912.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14700) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (969, 1540, 2395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5247) [M::mem_pestat] mean and std.dev: (1721.06, 946.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6673) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1014.131 CPU sec, 47.402 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1035757, 26, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (674, 1583, 2803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7061) [M::mem_pestat] mean and std.dev: (1585.07, 1271.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9190) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.13, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (144, 2585, 3645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10647) [M::mem_pestat] mean and std.dev: (2649.81, 2833.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14148) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1076, 1364, 2093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4127) [M::mem_pestat] mean and std.dev: (1452.40, 666.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5144) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1014.312 CPU sec, 47.563 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 1036709, 31, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (557, 1041, 2685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6941) [M::mem_pestat] mean and std.dev: (1493.47, 1277.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9069) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.13, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (564, 2581, 4998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13866) [M::mem_pestat] mean and std.dev: (3223.19, 3048.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18300) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (883, 1679, 2814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6676) [M::mem_pestat] mean and std.dev: (2112.75, 1738.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9065) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1009.584 CPU sec, 44.460 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1037177, 23, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (561, 783, 2975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7803) [M::mem_pestat] mean and std.dev: (1997.74, 2155.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (168, 1328, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7482) [M::mem_pestat] mean and std.dev: (1564.64, 1675.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9920) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (855, 1562, 2487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5751) [M::mem_pestat] mean and std.dev: (1282.80, 778.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7383) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1012.531 CPU sec, 50.864 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1036303, 36, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (368, 396, 1684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4316) [M::mem_pestat] mean and std.dev: (866.38, 824.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (176, 1975, 6305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18563) [M::mem_pestat] mean and std.dev: (2950.47, 3208.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 24692) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (895, 1474, 2632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6106) [M::mem_pestat] mean and std.dev: (1567.67, 1216.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7843) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1012.148 CPU sec, 44.620 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1036613, 26, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (353, 598, 1212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2930) [M::mem_pestat] mean and std.dev: (719.89, 443.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (406, 2107, 3081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8431) [M::mem_pestat] mean and std.dev: (1901.74, 1910.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11106) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1261, 1950, 4175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10003) [M::mem_pestat] mean and std.dev: (2863.40, 2293.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12917) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1030.492 CPU sec, 67.388 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036401, 34, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (493, 992, 2694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7096) [M::mem_pestat] mean and std.dev: (1371.61, 1293.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9297) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (406, 1949, 3610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10018) [M::mem_pestat] mean and std.dev: (2735.47, 2732.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13664) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (427, 1242, 1832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4642) [M::mem_pestat] mean and std.dev: (1209.64, 981.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6047) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1013.391 CPU sec, 47.267 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1037441, 37, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (411, 698, 1949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5025) [M::mem_pestat] mean and std.dev: (982.94, 980.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.82) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (686, 2605, 8509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 24155) [M::mem_pestat] mean and std.dev: (3738.57, 3304.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 31978) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (747, 1309, 1687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3567) [M::mem_pestat] mean and std.dev: (1292.94, 871.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4778) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1006.257 CPU sec, 48.471 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 1036400, 29, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (326, 963, 1782) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4694) [M::mem_pestat] mean and std.dev: (1054.75, 797.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6150) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.10, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (278, 1742, 2952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8300) [M::mem_pestat] mean and std.dev: (1788.37, 1660.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10974) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (765, 949, 2384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5622) [M::mem_pestat] mean and std.dev: (1120.85, 791.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7241) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1011.553 CPU sec, 44.595 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1035516, 38, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (479, 1011, 2940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7862) [M::mem_pestat] mean and std.dev: (1415.35, 1560.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10323) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.08, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (261, 1870, 2958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8352) [M::mem_pestat] mean and std.dev: (1610.09, 1678.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11049) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (862, 2070, 5269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14083) [M::mem_pestat] mean and std.dev: (2838.00, 2669.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18490) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1010.416 CPU sec, 47.463 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 1037093, 26, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (376, 459, 1498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3742) [M::mem_pestat] mean and std.dev: (900.40, 866.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4864) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.87) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (314, 1652, 4090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11642) [M::mem_pestat] mean and std.dev: (2807.46, 3101.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15418) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (511, 1335, 3106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8296) [M::mem_pestat] mean and std.dev: (2150.88, 2068.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10891) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1008.510 CPU sec, 44.438 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1036735, 25, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (387, 1353, 4250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11976) [M::mem_pestat] mean and std.dev: (2341.16, 2407.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15839) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.86) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (762, 2600, 5093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13755) [M::mem_pestat] mean and std.dev: (3325.08, 2781.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18086) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1627, 1914, 4242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9472) [M::mem_pestat] mean and std.dev: (2530.92, 1932.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12087) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1028.184 CPU sec, 66.879 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1036603, 29, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (376, 649, 1250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2998) [M::mem_pestat] mean and std.dev: (652.77, 594.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3872) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.12, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (472, 1872, 2592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6832) [M::mem_pestat] mean and std.dev: (1637.07, 1418.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8952) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (836, 1577, 3033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7427) [M::mem_pestat] mean and std.dev: (1870.33, 1502.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9624) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1009.823 CPU sec, 46.756 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1037236, 39, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (368, 433, 1777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4595) [M::mem_pestat] mean and std.dev: (998.57, 940.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 493, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.07, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (662, 2069, 3409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8903) [M::mem_pestat] mean and std.dev: (2481.34, 2353.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11893) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1095, 3421, 8156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 22278) [M::mem_pestat] mean and std.dev: (4059.82, 3508.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 29339) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1010.870 CPU sec, 47.293 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1037017, 27, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (377, 1034, 2263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6035) [M::mem_pestat] mean and std.dev: (1199.78, 993.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1172, 2263, 3893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9335) [M::mem_pestat] mean and std.dev: (2963.93, 2776.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14071) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (813, 1424, 2378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5508) [M::mem_pestat] mean and std.dev: (1362.89, 834.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7073) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1018.983 CPU sec, 45.003 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1035408, 41, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (395, 426, 1128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2594) [M::mem_pestat] mean and std.dev: (662.82, 449.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.09, 17.84) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (185, 2266, 3598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10424) [M::mem_pestat] mean and std.dev: (2520.76, 2658.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13837) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (470, 1017, 1878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4694) [M::mem_pestat] mean and std.dev: (1020.82, 639.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6102) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1014.773 CPU sec, 50.111 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1036862, 39, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (309, 380, 1044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2514) [M::mem_pestat] mean and std.dev: (596.70, 559.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3249) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.83) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (339, 2174, 4692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13398) [M::mem_pestat] mean and std.dev: (2808.21, 2939.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17751) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (457, 840, 1746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4324) [M::mem_pestat] mean and std.dev: (1061.69, 792.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5613) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1017.792 CPU sec, 44.832 real sec [M::process] read 2464276 sequences (221784840 bp)... [M::process] 0 single-end sequences; 2464276 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 958390, 38, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (342, 409, 1012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2352) [M::mem_pestat] mean and std.dev: (616.70, 363.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3022) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (480, 494, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (426, 561) [M::mem_pestat] mean and std.dev: (494.11, 17.85) [M::mem_pestat] low and high boundaries for proper pairs: (399, 588) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (351, 1384, 2858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7872) [M::mem_pestat] mean and std.dev: (1887.56, 1994.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10379) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2464276 reads in 933.043 CPU sec, 40.423 real sec [main] Version: 0.7.17-r1188 [main] CMD: /home/csyk2proj/tools/bwa.kit/bwa mem -p -t24 -R@RG\tID:KPGP-00122\tPL:ILLUMINA\tSM:KPGP-00122 -C /home/csyk2proj/data/bwa.kit/hs38DH.fa - [main] Real time: 45299.701 sec; CPU: 449400.787 sec