[M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1028764, 48, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (298, 356, 773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1723) [M::mem_pestat] mean and std.dev: (462.32, 336.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2198) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (487, 2590, 2611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6859) [M::mem_pestat] mean and std.dev: (1923.72, 1484.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8983) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (427, 824, 1575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3871) [M::mem_pestat] mean and std.dev: (755.50, 424.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5019) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1289.339 CPU sec, 77.310 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 1028941, 39, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (335, 439, 1462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3716) [M::mem_pestat] mean and std.dev: (844.44, 832.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4843) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (562, 2523, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6694) [M::mem_pestat] mean and std.dev: (1867.41, 1376.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8738) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1206.681 CPU sec, 55.247 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1028839, 47, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 388, 1641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4299) [M::mem_pestat] mean and std.dev: (747.73, 678.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1659, 2592, 2880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5322) [M::mem_pestat] mean and std.dev: (2014.60, 1285.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7156) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (784, 1090, 1484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2884) [M::mem_pestat] mean and std.dev: (911.75, 309.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3584) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1219.273 CPU sec, 66.617 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1029869, 52, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (286, 327, 491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (331.94, 109.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1106) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (356, 1895, 2602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7094) [M::mem_pestat] mean and std.dev: (1577.12, 1316.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9340) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1208.143 CPU sec, 53.458 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1029158, 48, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 429, 2738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7564) [M::mem_pestat] mean and std.dev: (1541.90, 1833.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9977) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (261, 2054, 2613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7317) [M::mem_pestat] mean and std.dev: (1710.09, 1531.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9669) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (713, 1950, 2760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6854) [M::mem_pestat] mean and std.dev: (2091.86, 1645.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8901) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1229.632 CPU sec, 69.637 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1029615, 42, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (333, 351, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (7, 822) [M::mem_pestat] mean and std.dev: (349.88, 81.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 985) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1139, 2589, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5537) [M::mem_pestat] mean and std.dev: (1886.83, 1083.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7003) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (898, 1436, 2871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6817) [M::mem_pestat] mean and std.dev: (1739.60, 1503.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8790) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1203.392 CPU sec, 53.067 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1029073, 49, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 347, 702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1472) [M::mem_pestat] mean and std.dev: (332.00, 164.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1857) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (287, 2589, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7226) [M::mem_pestat] mean and std.dev: (1784.15, 1276.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9539) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1171, 1450, 3726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8836) [M::mem_pestat] mean and std.dev: (2382.00, 2124.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11391) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1270.140 CPU sec, 75.508 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1028570, 52, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (370, 747, 1795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4645) [M::mem_pestat] mean and std.dev: (994.79, 903.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6070) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (263, 1968, 2601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7277) [M::mem_pestat] mean and std.dev: (1628.92, 1424.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9615) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (626, 952, 2997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7739) [M::mem_pestat] mean and std.dev: (1713.08, 1859.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10110) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1230.506 CPU sec, 56.221 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1028616, 37, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 328, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988) [M::mem_pestat] mean and std.dev: (310.20, 75.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1220) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (404, 2582, 2613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7031) [M::mem_pestat] mean and std.dev: (1714.03, 1231.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9240) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1226.039 CPU sec, 67.487 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1029744, 56, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (303, 337, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (139, 549) [M::mem_pestat] mean and std.dev: (314.56, 52.48) [M::mem_pestat] low and high boundaries for proper pairs: (57, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (266, 2583, 2611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7301) [M::mem_pestat] mean and std.dev: (1963.77, 1554.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9646) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (859, 1200, 1359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2359) [M::mem_pestat] mean and std.dev: (1052.80, 501.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3061) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1217.719 CPU sec, 53.708 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1028675, 59, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 368, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (55, 710) [M::mem_pestat] mean and std.dev: (348.33, 69.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (625, 2588, 2614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6592) [M::mem_pestat] mean and std.dev: (2095.04, 1499.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8581) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (457, 849, 2129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5473) [M::mem_pestat] mean and std.dev: (1283.81, 1242.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7145) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1219.178 CPU sec, 67.523 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1029099, 55, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 355, 714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1646) [M::mem_pestat] mean and std.dev: (311.47, 165.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2112) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (688, 2586, 2612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6460) [M::mem_pestat] mean and std.dev: (1918.17, 1223.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8384) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (573, 986, 1892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4530) [M::mem_pestat] mean and std.dev: (1113.67, 736.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5849) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1203.768 CPU sec, 53.101 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1028925, 56, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (333, 372, 2286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6192) [M::mem_pestat] mean and std.dev: (1054.56, 997.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8145) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (430, 2580, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6961) [M::mem_pestat] mean and std.dev: (1835.02, 1503.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9138) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (544, 1114, 1595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3697) [M::mem_pestat] mean and std.dev: (1016.07, 530.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4748) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1274.087 CPU sec, 75.771 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1029846, 38, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (280, 327, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (128, 508) [M::mem_pestat] mean and std.dev: (313.68, 50.36) [M::mem_pestat] low and high boundaries for proper pairs: (52, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (824, 2592, 2623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6221) [M::mem_pestat] mean and std.dev: (1960.49, 1235.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8020) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1214.498 CPU sec, 55.628 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1028991, 44, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (295, 352, 671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1423) [M::mem_pestat] mean and std.dev: (396.40, 273.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (281, 2595, 3635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10343) [M::mem_pestat] mean and std.dev: (2776.52, 2608.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13697) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (946, 996, 1788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3472) [M::mem_pestat] mean and std.dev: (1207.69, 524.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4314) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1228.065 CPU sec, 66.093 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1029098, 33, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (301, 339, 1179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2935) [M::mem_pestat] mean and std.dev: (641.17, 702.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3813) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (249, 2585, 2608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7326) [M::mem_pestat] mean and std.dev: (1774.90, 1314.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9685) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (359, 948, 2878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7916) [M::mem_pestat] mean and std.dev: (1275.00, 1122.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10435) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1226.054 CPU sec, 54.117 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1028927, 51, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (348, 384, 1176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2832) [M::mem_pestat] mean and std.dev: (784.80, 707.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.46, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (279, 2586, 2617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7293) [M::mem_pestat] mean and std.dev: (1663.91, 1207.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9631) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (833, 1174, 5820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15794) [M::mem_pestat] mean and std.dev: (2604.69, 2269.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20781) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1245.372 CPU sec, 73.505 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1027696, 49, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (333, 445, 1436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3642) [M::mem_pestat] mean and std.dev: (746.19, 767.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.45) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (440, 2599, 3397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9311) [M::mem_pestat] mean and std.dev: (3101.24, 2851.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14508) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (394, 891, 1389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3379) [M::mem_pestat] mean and std.dev: (1084.17, 947.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4875) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1223.289 CPU sec, 54.052 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1030063, 49, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (272, 358, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1211) [M::mem_pestat] mean and std.dev: (381.82, 165.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (336, 2573, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7128) [M::mem_pestat] mean and std.dev: (1771.96, 1457.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9392) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1094, 1210, 2220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4472) [M::mem_pestat] mean and std.dev: (1252.22, 585.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5598) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1260.214 CPU sec, 75.438 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1029248, 53, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (321, 611, 1166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2856) [M::mem_pestat] mean and std.dev: (650.78, 434.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (369, 2587, 2857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7833) [M::mem_pestat] mean and std.dev: (1910.85, 1490.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10321) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (600, 1451, 5399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14997) [M::mem_pestat] mean and std.dev: (2456.75, 2386.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19796) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1210.500 CPU sec, 54.982 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 1030035, 59, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (351, 359, 1572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4014) [M::mem_pestat] mean and std.dev: (719.40, 645.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5235) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (217, 1872, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7375) [M::mem_pestat] mean and std.dev: (1651.62, 1588.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9761) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1223.869 CPU sec, 68.405 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1028258, 46, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (310, 388, 952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2236) [M::mem_pestat] mean and std.dev: (588.17, 465.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (242, 2015, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7325) [M::mem_pestat] mean and std.dev: (1888.52, 1556.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9686) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1226.347 CPU sec, 54.237 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1029255, 54, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (332, 518, 1260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3116) [M::mem_pestat] mean and std.dev: (884.06, 761.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4044) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (378, 2582, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7053) [M::mem_pestat] mean and std.dev: (1839.88, 1498.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9278) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (430, 932, 2103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5449) [M::mem_pestat] mean and std.dev: (1375.08, 1312.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7122) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1244.600 CPU sec, 69.969 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 1030609, 42, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 340, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (14, 789) [M::mem_pestat] mean and std.dev: (345.17, 164.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (702, 2051, 2602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6402) [M::mem_pestat] mean and std.dev: (1747.38, 1073.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8302) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (361, 816, 1234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2980) [M::mem_pestat] mean and std.dev: (697.83, 379.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3853) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1216.925 CPU sec, 53.561 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 1029519, 44, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 409, 750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1864) [M::mem_pestat] mean and std.dev: (460.00, 399.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1476, 2592, 2602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4854) [M::mem_pestat] mean and std.dev: (2123.53, 1181.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6848) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (918, 1502, 2671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6177) [M::mem_pestat] mean and std.dev: (1409.11, 858.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7930) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1272.417 CPU sec, 76.133 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1029214, 49, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 358, 984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2386) [M::mem_pestat] mean and std.dev: (582.86, 555.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3087) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (280, 2583, 2803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7849) [M::mem_pestat] mean and std.dev: (1905.36, 1707.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10372) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1221.558 CPU sec, 55.387 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1029786, 41, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (323, 377, 1540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3974) [M::mem_pestat] mean and std.dev: (877.18, 904.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (479, 2584, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6851) [M::mem_pestat] mean and std.dev: (1787.78, 1214.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8975) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (792, 1144, 4513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11955) [M::mem_pestat] mean and std.dev: (2058.31, 2057.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15676) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1230.711 CPU sec, 70.807 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1029720, 46, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (279, 338, 1063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2631) [M::mem_pestat] mean and std.dev: (595.41, 549.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (264, 2588, 2610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7302) [M::mem_pestat] mean and std.dev: (1788.21, 1391.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9648) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1223.842 CPU sec, 54.150 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 1029226, 64, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 364, 1006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2350) [M::mem_pestat] mean and std.dev: (566.62, 464.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3022) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (601, 2586, 2966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7696) [M::mem_pestat] mean and std.dev: (1887.47, 1497.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10061) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (739, 1373, 2384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5674) [M::mem_pestat] mean and std.dev: (1525.75, 1274.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7319) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1240.094 CPU sec, 67.634 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1028634, 50, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (331, 375, 1567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4039) [M::mem_pestat] mean and std.dev: (780.28, 905.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5275) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (260, 2585, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7295) [M::mem_pestat] mean and std.dev: (1787.65, 1316.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9640) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1217.798 CPU sec, 53.685 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1029228, 62, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 360, 654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1364) [M::mem_pestat] mean and std.dev: (413.88, 228.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1224, 2594, 2610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5382) [M::mem_pestat] mean and std.dev: (1961.60, 1151.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6768) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1265.259 CPU sec, 75.725 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1028299, 55, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (320, 535, 1791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4733) [M::mem_pestat] mean and std.dev: (1204.84, 1394.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (856, 2241, 2609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6115) [M::mem_pestat] mean and std.dev: (1799.71, 1287.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7868) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1231.011 CPU sec, 56.487 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 1029086, 39, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 387, 1109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2647) [M::mem_pestat] mean and std.dev: (671.69, 629.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (273, 2591, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7269) [M::mem_pestat] mean and std.dev: (1589.44, 1340.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9601) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1233.768 CPU sec, 66.078 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1028954, 42, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (261, 359, 933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2277) [M::mem_pestat] mean and std.dev: (580.90, 477.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2949) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (658, 2596, 2957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7555) [M::mem_pestat] mean and std.dev: (1996.97, 1612.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9854) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (371, 1228, 2321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6221) [M::mem_pestat] mean and std.dev: (1325.33, 1456.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8171) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1219.873 CPU sec, 53.690 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1029289, 38, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (353, 884, 3219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8951) [M::mem_pestat] mean and std.dev: (1499.53, 1771.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11817) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1676, 2597, 5684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13700) [M::mem_pestat] mean and std.dev: (3567.92, 3038.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17708) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (450, 1084, 5835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16605) [M::mem_pestat] mean and std.dev: (2240.69, 2426.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 21990) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1216.517 CPU sec, 68.684 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1028890, 54, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (290, 337, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (152, 497) [M::mem_pestat] mean and std.dev: (312.33, 40.11) [M::mem_pestat] low and high boundaries for proper pairs: (83, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (307, 2591, 2610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7216) [M::mem_pestat] mean and std.dev: (1973.18, 1572.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9519) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1226.219 CPU sec, 54.259 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1028636, 56, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (356, 386, 952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2144) [M::mem_pestat] mean and std.dev: (677.69, 506.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.45) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (428, 2330, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6953) [M::mem_pestat] mean and std.dev: (1803.10, 1391.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9128) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1271.682 CPU sec, 75.639 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1028757, 46, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (326, 362, 2455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6713) [M::mem_pestat] mean and std.dev: (1287.86, 1687.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1265, 2593, 2610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5300) [M::mem_pestat] mean and std.dev: (2020.34, 1267.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7090) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (391, 1033, 1250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2968) [M::mem_pestat] mean and std.dev: (741.22, 459.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3827) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1222.112 CPU sec, 55.747 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1028439, 41, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (313, 357, 944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2206) [M::mem_pestat] mean and std.dev: (394.39, 253.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (275, 1099, 2601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7253) [M::mem_pestat] mean and std.dev: (1455.97, 1374.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9579) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (415, 1008, 2454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6532) [M::mem_pestat] mean and std.dev: (1461.46, 1335.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8571) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1220.104 CPU sec, 67.165 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1029073, 44, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (322, 367, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (84, 679) [M::mem_pestat] mean and std.dev: (346.62, 75.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (330, 2584, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7161) [M::mem_pestat] mean and std.dev: (1836.28, 1384.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9438) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1216.305 CPU sec, 53.712 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1028925, 47, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 338, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (281.05, 166.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1065) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (256, 2591, 2643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7417) [M::mem_pestat] mean and std.dev: (2072.00, 1822.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9804) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (866, 1345, 2839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6785) [M::mem_pestat] mean and std.dev: (1992.71, 1929.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9710) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1245.137 CPU sec, 69.737 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 1029288, 46, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (318, 368, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062) [M::mem_pestat] mean and std.dev: (381.74, 124.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1310) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.50, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (234, 2583, 2611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7365) [M::mem_pestat] mean and std.dev: (1650.30, 1356.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9742) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (448, 722, 2958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7978) [M::mem_pestat] mean and std.dev: (1669.29, 1605.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10488) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1221.110 CPU sec, 53.983 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1028950, 56, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (318, 358, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (168, 543) [M::mem_pestat] mean and std.dev: (342.69, 65.57) [M::mem_pestat] low and high boundaries for proper pairs: (80, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.50, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (355, 2563, 2609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7117) [M::mem_pestat] mean and std.dev: (1824.81, 1318.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9371) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1205.948 CPU sec, 53.204 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1029230, 43, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (336, 447, 1738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4542) [M::mem_pestat] mean and std.dev: (1041.20, 1165.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (555, 2589, 3675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9915) [M::mem_pestat] mean and std.dev: (2731.60, 2634.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13268) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1273.514 CPU sec, 75.610 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1030035, 46, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (288, 358, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (76, 606) [M::mem_pestat] mean and std.dev: (323.72, 90.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (414, 2509, 2599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6969) [M::mem_pestat] mean and std.dev: (1620.51, 1134.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9154) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (765, 1041, 1656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3438) [M::mem_pestat] mean and std.dev: (1101.45, 504.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4329) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1220.607 CPU sec, 57.697 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 1028868, 50, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 378, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1221) [M::mem_pestat] mean and std.dev: (342.42, 142.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (400, 2103, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7015) [M::mem_pestat] mean and std.dev: (1837.96, 1386.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9220) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (766, 1375, 3208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8092) [M::mem_pestat] mean and std.dev: (1451.83, 991.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10534) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1235.453 CPU sec, 70.815 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1029427, 41, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (288, 355, 1129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2811) [M::mem_pestat] mean and std.dev: (673.13, 777.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (266, 2576, 2618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7322) [M::mem_pestat] mean and std.dev: (1789.64, 1627.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9674) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (574, 882, 1496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3340) [M::mem_pestat] mean and std.dev: (1001.90, 752.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4262) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1220.552 CPU sec, 54.159 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1029280, 50, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 386, 577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1083) [M::mem_pestat] mean and std.dev: (432.67, 177.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1179, 2586, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5460) [M::mem_pestat] mean and std.dev: (1990.74, 1179.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6887) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (793, 1410, 3316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8362) [M::mem_pestat] mean and std.dev: (1977.69, 1839.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10885) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1231.237 CPU sec, 69.749 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1029389, 57, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 347, 1564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4094) [M::mem_pestat] mean and std.dev: (727.83, 783.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1134, 2592, 4282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10578) [M::mem_pestat] mean and std.dev: (3138.77, 2779.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14255) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (813, 1459, 2958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7248) [M::mem_pestat] mean and std.dev: (1796.50, 1401.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9393) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1217.150 CPU sec, 53.660 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 1029421, 46, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (288, 354, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (144, 504) [M::mem_pestat] mean and std.dev: (316.04, 71.86) [M::mem_pestat] low and high boundaries for proper pairs: (29, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (243, 1906, 2897) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8205) [M::mem_pestat] mean and std.dev: (1813.84, 1480.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10859) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1259.056 CPU sec, 75.899 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1029419, 47, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (335, 372, 1025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2405) [M::mem_pestat] mean and std.dev: (562.26, 364.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3095) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (310, 2465, 2609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7207) [M::mem_pestat] mean and std.dev: (1921.51, 1689.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9506) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1219.758 CPU sec, 55.638 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1029460, 42, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (326, 360, 840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1868) [M::mem_pestat] mean and std.dev: (381.81, 186.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.50, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (263, 2134, 2602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7280) [M::mem_pestat] mean and std.dev: (1469.76, 1171.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9619) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1227.695 CPU sec, 64.477 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1029710, 50, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 357, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (302.25, 99.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1127) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (146, 460, 2597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7499) [M::mem_pestat] mean and std.dev: (1157.19, 1246.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9950) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (418, 1012, 3210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8794) [M::mem_pestat] mean and std.dev: (1821.41, 1806.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11586) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1210.154 CPU sec, 53.342 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1028730, 46, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 357, 1402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3556) [M::mem_pestat] mean and std.dev: (872.12, 968.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (334, 2589, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7147) [M::mem_pestat] mean and std.dev: (1919.60, 1470.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9418) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1240.773 CPU sec, 69.355 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1029637, 52, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (296, 361, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1295) [M::mem_pestat] mean and std.dev: (373.57, 169.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1883, 2594, 3978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8168) [M::mem_pestat] mean and std.dev: (2557.23, 1741.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10263) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1218.883 CPU sec, 53.811 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1028757, 56, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (347, 375, 1026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2384) [M::mem_pestat] mean and std.dev: (636.17, 559.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3063) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (282, 2317, 2601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7239) [M::mem_pestat] mean and std.dev: (1609.14, 1191.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9558) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (881, 1064, 1876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3866) [M::mem_pestat] mean and std.dev: (1147.88, 722.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4861) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1257.509 CPU sec, 75.367 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1029297, 37, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 398, 1103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2675) [M::mem_pestat] mean and std.dev: (576.76, 417.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (375, 2155, 2852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7806) [M::mem_pestat] mean and std.dev: (1986.19, 1702.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10283) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1221.139 CPU sec, 56.074 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1029960, 50, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (342, 395, 1535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3921) [M::mem_pestat] mean and std.dev: (886.17, 805.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5114) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (263, 2546, 2602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7280) [M::mem_pestat] mean and std.dev: (1737.83, 1345.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9619) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (917, 1344, 2242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4892) [M::mem_pestat] mean and std.dev: (1541.56, 1263.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6598) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1220.363 CPU sec, 62.036 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1028779, 50, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 327, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (194, 489) [M::mem_pestat] mean and std.dev: (315.07, 35.77) [M::mem_pestat] low and high boundaries for proper pairs: (135, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (260, 2588, 2950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8330) [M::mem_pestat] mean and std.dev: (2148.85, 1919.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11020) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (637, 1432, 2548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6370) [M::mem_pestat] mean and std.dev: (1384.40, 952.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8281) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1216.346 CPU sec, 53.609 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 1029397, 34, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (371, 472, 1793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4637) [M::mem_pestat] mean and std.dev: (941.08, 889.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6059) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (261, 1394, 2593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7257) [M::mem_pestat] mean and std.dev: (1417.50, 1298.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9589) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1236.057 CPU sec, 70.843 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1029133, 41, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 356, 1504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3966) [M::mem_pestat] mean and std.dev: (680.69, 610.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5197) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1062, 2588, 2848) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6420) [M::mem_pestat] mean and std.dev: (1982.30, 1492.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8206) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1023, 1307, 2234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4656) [M::mem_pestat] mean and std.dev: (1321.00, 738.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5867) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1229.690 CPU sec, 54.454 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1029082, 48, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 337, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (199, 494) [M::mem_pestat] mean and std.dev: (330.61, 37.17) [M::mem_pestat] low and high boundaries for proper pairs: (140, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.46, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (374, 2588, 2906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7970) [M::mem_pestat] mean and std.dev: (2056.70, 1930.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10502) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (402, 1058, 1344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3228) [M::mem_pestat] mean and std.dev: (937.08, 592.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4170) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1276.560 CPU sec, 75.860 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1029504, 60, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 329, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (41, 621) [M::mem_pestat] mean and std.dev: (280.12, 73.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (640, 2594, 2608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6544) [M::mem_pestat] mean and std.dev: (1965.83, 1338.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (905, 1223, 2303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5099) [M::mem_pestat] mean and std.dev: (1476.00, 900.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6497) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1233.942 CPU sec, 56.362 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1029617, 50, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 376, 870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1986) [M::mem_pestat] mean and std.dev: (391.82, 202.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (818, 2587, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6164) [M::mem_pestat] mean and std.dev: (1953.77, 1441.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7946) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (505, 1201, 2298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5884) [M::mem_pestat] mean and std.dev: (1183.80, 734.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7677) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1229.689 CPU sec, 63.928 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1028390, 49, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (349, 1272, 2187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5863) [M::mem_pestat] mean and std.dev: (1439.69, 1498.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (342, 1895, 2614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7158) [M::mem_pestat] mean and std.dev: (1854.23, 1359.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9430) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1218.764 CPU sec, 53.667 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1029158, 64, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 351, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (87, 632) [M::mem_pestat] mean and std.dev: (329.40, 71.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (260, 2569, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7298) [M::mem_pestat] mean and std.dev: (1797.70, 1479.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9644) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1224.825 CPU sec, 67.583 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1029667, 51, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 426, 1852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4906) [M::mem_pestat] mean and std.dev: (791.68, 682.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (970, 2583, 2601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5863) [M::mem_pestat] mean and std.dev: (1889.80, 1178.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7494) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (791, 1362, 3015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7463) [M::mem_pestat] mean and std.dev: (1765.08, 1527.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9687) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1230.127 CPU sec, 54.347 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1029958, 50, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (323, 369, 1462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3740) [M::mem_pestat] mean and std.dev: (722.59, 585.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.45) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (305, 1499, 2595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7175) [M::mem_pestat] mean and std.dev: (1637.67, 1484.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9465) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (581, 987, 3261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8621) [M::mem_pestat] mean and std.dev: (1586.17, 1657.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11301) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1273.694 CPU sec, 76.171 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 1028851, 52, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 329, 982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2336) [M::mem_pestat] mean and std.dev: (492.93, 388.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3013) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.51, 11.38) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (136, 2593, 2613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7567) [M::mem_pestat] mean and std.dev: (2015.00, 1463.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10044) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1234.567 CPU sec, 56.411 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1028622, 51, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 349, 2233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6039) [M::mem_pestat] mean and std.dev: (971.90, 1103.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7942) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1017, 2583, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5775) [M::mem_pestat] mean and std.dev: (1749.39, 1126.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7361) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1229.606 CPU sec, 66.340 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1029856, 48, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (300, 363, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (152, 522) [M::mem_pestat] mean and std.dev: (348.71, 56.97) [M::mem_pestat] low and high boundaries for proper pairs: (78, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (503, 1928, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6794) [M::mem_pestat] mean and std.dev: (1629.36, 1148.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8891) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1218.683 CPU sec, 53.804 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1029682, 41, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (339, 1092, 2741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7545) [M::mem_pestat] mean and std.dev: (1447.95, 1420.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9947) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1820, 2594, 3614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7202) [M::mem_pestat] mean and std.dev: (2399.03, 1218.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8996) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1090, 1862, 2574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5542) [M::mem_pestat] mean and std.dev: (1359.67, 908.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7026) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1211.230 CPU sec, 67.655 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1029348, 48, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (332, 378, 843) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1865) [M::mem_pestat] mean and std.dev: (490.75, 290.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2376) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (710, 2594, 2867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7181) [M::mem_pestat] mean and std.dev: (2215.02, 1731.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9338) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (730, 1186, 2383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5689) [M::mem_pestat] mean and std.dev: (1468.11, 997.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7342) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1206.161 CPU sec, 53.121 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1029940, 34, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (310, 348, 1724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4552) [M::mem_pestat] mean and std.dev: (529.50, 403.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5966) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (682, 2590, 2957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7507) [M::mem_pestat] mean and std.dev: (2029.45, 1421.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9782) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (825, 1224, 1525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2925) [M::mem_pestat] mean and std.dev: (1115.19, 635.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3658) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1251.533 CPU sec, 74.723 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1029459, 53, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (288, 359, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1254) [M::mem_pestat] mean and std.dev: (418.87, 277.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.46, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1068, 2593, 2958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6738) [M::mem_pestat] mean and std.dev: (1942.29, 1362.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8628) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1227.718 CPU sec, 56.266 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1028601, 50, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 345, 921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2217) [M::mem_pestat] mean and std.dev: (431.57, 360.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2865) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (265, 2587, 2599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7267) [M::mem_pestat] mean and std.dev: (1820.26, 1441.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9601) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1224.281 CPU sec, 69.555 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1028852, 37, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (288, 366, 979) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2361) [M::mem_pestat] mean and std.dev: (644.14, 575.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3052) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1902, 2588, 2921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4959) [M::mem_pestat] mean and std.dev: (2194.76, 1239.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7151) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (651, 1829, 2786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7056) [M::mem_pestat] mean and std.dev: (1763.00, 1187.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1219.607 CPU sec, 53.917 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1029045, 46, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (304, 341, 1329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3379) [M::mem_pestat] mean and std.dev: (666.52, 740.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4404) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (76, 647, 2595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7633) [M::mem_pestat] mean and std.dev: (1302.51, 1468.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10152) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (769, 959, 1936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4270) [M::mem_pestat] mean and std.dev: (1357.67, 1012.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5437) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1238.688 CPU sec, 65.897 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1028692, 45, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (280, 354, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027) [M::mem_pestat] mean and std.dev: (349.32, 118.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1276) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (109, 1921, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7591) [M::mem_pestat] mean and std.dev: (1584.28, 1300.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10085) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (517, 1176, 1818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4420) [M::mem_pestat] mean and std.dev: (929.20, 593.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5721) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1215.835 CPU sec, 53.549 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1030154, 49, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 350, 841) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1891) [M::mem_pestat] mean and std.dev: (441.65, 292.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (308, 2586, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7202) [M::mem_pestat] mean and std.dev: (1904.15, 1505.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9500) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1053, 1358, 1640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2814) [M::mem_pestat] mean and std.dev: (1251.73, 459.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3401) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1265.901 CPU sec, 75.654 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1028873, 45, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (366, 658, 1345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3303) [M::mem_pestat] mean and std.dev: (902.08, 670.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4282) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (271, 1909, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7273) [M::mem_pestat] mean and std.dev: (1788.93, 1434.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9607) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1222.628 CPU sec, 55.821 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1029320, 58, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (358, 496, 1551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3937) [M::mem_pestat] mean and std.dev: (1016.48, 925.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5130) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (361, 2574, 2602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7084) [M::mem_pestat] mean and std.dev: (1798.84, 1292.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9325) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (331, 871, 6288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18202) [M::mem_pestat] mean and std.dev: (2643.25, 2842.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 24159) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1242.431 CPU sec, 70.820 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 1028194, 47, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (342, 536, 2078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5550) [M::mem_pestat] mean and std.dev: (1166.23, 1049.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7286) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.45) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1208, 2592, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5405) [M::mem_pestat] mean and std.dev: (1866.83, 1101.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6804) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (453, 1193, 2185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5649) [M::mem_pestat] mean and std.dev: (1373.60, 1456.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7381) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1217.689 CPU sec, 53.785 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1028231, 45, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (282, 369, 1508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3960) [M::mem_pestat] mean and std.dev: (785.57, 968.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5186) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (126, 2569, 2609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7575) [M::mem_pestat] mean and std.dev: (1945.60, 1899.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10058) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (427, 982, 2712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7282) [M::mem_pestat] mean and std.dev: (1552.00, 1514.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9567) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1231.625 CPU sec, 71.832 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1029485, 43, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (313, 451, 1394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3556) [M::mem_pestat] mean and std.dev: (888.92, 900.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (422, 2517, 2604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6968) [M::mem_pestat] mean and std.dev: (1733.08, 1297.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9150) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (970, 1763, 2347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5101) [M::mem_pestat] mean and std.dev: (1472.55, 750.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6478) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1218.843 CPU sec, 53.865 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1028518, 37, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (332, 364, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (118, 653) [M::mem_pestat] mean and std.dev: (358.33, 101.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 766) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (276, 1907, 2899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8145) [M::mem_pestat] mean and std.dev: (1915.57, 1787.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10768) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (372, 1132, 1700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4356) [M::mem_pestat] mean and std.dev: (1039.31, 680.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5684) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1215.136 CPU sec, 54.930 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1028267, 48, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (318, 352, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (198, 498) [M::mem_pestat] mean and std.dev: (333.24, 36.71) [M::mem_pestat] low and high boundaries for proper pairs: (138, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1223, 2592, 2614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5396) [M::mem_pestat] mean and std.dev: (2024.54, 1331.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7352) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (543, 1054, 2123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5283) [M::mem_pestat] mean and std.dev: (1390.57, 1064.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6863) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1250.802 CPU sec, 78.132 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 1028844, 56, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (358, 382, 802) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1690) [M::mem_pestat] mean and std.dev: (459.73, 298.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2134) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.46, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1017, 2463, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5781) [M::mem_pestat] mean and std.dev: (1757.45, 1089.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7369) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1131, 1823, 2514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5280) [M::mem_pestat] mean and std.dev: (1582.67, 685.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6663) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1215.350 CPU sec, 54.591 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1028617, 60, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (355, 509, 1494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3772) [M::mem_pestat] mean and std.dev: (867.39, 839.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1597, 2593, 2616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4654) [M::mem_pestat] mean and std.dev: (2014.23, 1107.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6445) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1231.704 CPU sec, 68.098 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1029309, 41, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (306, 359, 745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1623) [M::mem_pestat] mean and std.dev: (325.23, 154.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2062) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1374, 2588, 2799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5649) [M::mem_pestat] mean and std.dev: (2018.61, 1281.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7143) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (642, 1202, 1262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2502) [M::mem_pestat] mean and std.dev: (893.33, 357.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3122) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1223.010 CPU sec, 54.018 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1029182, 45, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (322, 353, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (176, 541) [M::mem_pestat] mean and std.dev: (335.31, 48.79) [M::mem_pestat] low and high boundaries for proper pairs: (103, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (81, 1036, 2598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7632) [M::mem_pestat] mean and std.dev: (1553.73, 1491.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10149) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1235.755 CPU sec, 67.908 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1030305, 51, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 360, 2592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7306) [M::mem_pestat] mean and std.dev: (1216.57, 1591.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (269, 2573, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7283) [M::mem_pestat] mean and std.dev: (1780.72, 1383.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9621) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (407, 1003, 1565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3881) [M::mem_pestat] mean and std.dev: (1080.77, 958.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5039) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1212.176 CPU sec, 53.520 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1028669, 43, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (296, 375, 1092) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2684) [M::mem_pestat] mean and std.dev: (673.41, 567.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (412, 2598, 3615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10021) [M::mem_pestat] mean and std.dev: (2832.05, 2454.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13224) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1271.344 CPU sec, 75.922 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1029171, 45, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 343, 1085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2773) [M::mem_pestat] mean and std.dev: (389.61, 393.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (462, 2592, 2942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7902) [M::mem_pestat] mean and std.dev: (2450.60, 2164.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11109) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1225.028 CPU sec, 55.904 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1028748, 56, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 385, 1209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2993) [M::mem_pestat] mean and std.dev: (518.67, 416.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3885) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (363, 2584, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7092) [M::mem_pestat] mean and std.dev: (1793.86, 1392.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9335) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1236.471 CPU sec, 67.607 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 1029213, 56, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 337, 1620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4298) [M::mem_pestat] mean and std.dev: (784.09, 872.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (252, 2567, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7296) [M::mem_pestat] mean and std.dev: (1506.70, 1234.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9644) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (345, 824, 1761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4593) [M::mem_pestat] mean and std.dev: (1156.36, 981.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6009) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1215.763 CPU sec, 53.673 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1029501, 42, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (293, 344, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (27, 692) [M::mem_pestat] mean and std.dev: (328.76, 57.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1060, 2579, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5701) [M::mem_pestat] mean and std.dev: (1890.41, 1243.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7248) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (811, 1306, 2586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6136) [M::mem_pestat] mean and std.dev: (1275.36, 804.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7911) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1226.633 CPU sec, 68.496 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1029567, 60, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (346, 387, 943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2137) [M::mem_pestat] mean and std.dev: (567.95, 356.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2734) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (185, 1904, 2611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7463) [M::mem_pestat] mean and std.dev: (1778.73, 1672.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9889) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1218.148 CPU sec, 53.646 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1029459, 49, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (249, 340, 582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1248) [M::mem_pestat] mean and std.dev: (381.41, 218.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1117, 2594, 3734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8968) [M::mem_pestat] mean and std.dev: (2875.82, 2468.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12749) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (379, 1221, 1641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4165) [M::mem_pestat] mean and std.dev: (947.45, 681.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5427) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1258.802 CPU sec, 75.244 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1029609, 47, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (321, 356, 601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1161) [M::mem_pestat] mean and std.dev: (375.53, 145.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (811, 2583, 2651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6331) [M::mem_pestat] mean and std.dev: (1819.23, 1217.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8171) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (424, 740, 1203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2761) [M::mem_pestat] mean and std.dev: (764.10, 456.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3540) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1223.374 CPU sec, 55.714 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1029520, 52, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (313, 663, 1785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4729) [M::mem_pestat] mean and std.dev: (870.11, 926.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6201) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (895, 2584, 2676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6238) [M::mem_pestat] mean and std.dev: (1780.14, 1251.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8019) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (421, 1166, 2826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7636) [M::mem_pestat] mean and std.dev: (1631.13, 1752.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10041) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1216.728 CPU sec, 67.019 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 1028982, 38, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (336, 373, 1793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4707) [M::mem_pestat] mean and std.dev: (791.54, 700.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6164) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (699, 2596, 3031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7695) [M::mem_pestat] mean and std.dev: (2346.73, 1754.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10027) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (513, 880, 1831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4467) [M::mem_pestat] mean and std.dev: (1046.21, 668.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5785) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1194.941 CPU sec, 52.610 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1029525, 53, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 348, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (279.53, 110.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (222, 2582, 2614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7398) [M::mem_pestat] mean and std.dev: (1842.67, 1636.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9790) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (769, 1124, 3543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9091) [M::mem_pestat] mean and std.dev: (2151.59, 2110.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11865) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1208.944 CPU sec, 64.010 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1028949, 61, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 353, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (363.59, 189.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (285, 2046, 2637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7341) [M::mem_pestat] mean and std.dev: (1538.58, 1240.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9693) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1209.639 CPU sec, 53.366 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1029729, 44, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 1546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3962) [M::mem_pestat] mean and std.dev: (723.13, 566.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5170) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (272, 2061, 2976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8384) [M::mem_pestat] mean and std.dev: (1890.22, 1716.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11088) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (422, 1253, 2192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5732) [M::mem_pestat] mean and std.dev: (1229.75, 1299.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7502) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1271.506 CPU sec, 76.333 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1028680, 46, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (321, 360, 1139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2775) [M::mem_pestat] mean and std.dev: (599.82, 411.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (259, 2296, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7300) [M::mem_pestat] mean and std.dev: (1741.83, 1481.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9647) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (398, 754, 1737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4415) [M::mem_pestat] mean and std.dev: (1204.00, 1031.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5754) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1217.711 CPU sec, 55.440 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1029420, 42, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 335, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (91, 566) [M::mem_pestat] mean and std.dev: (321.86, 38.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (608, 2576, 2629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6671) [M::mem_pestat] mean and std.dev: (1843.19, 1478.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8692) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (475, 1010, 1236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2758) [M::mem_pestat] mean and std.dev: (893.69, 589.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3519) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1211.579 CPU sec, 63.764 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1029279, 60, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (343, 475, 1336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3322) [M::mem_pestat] mean and std.dev: (658.82, 465.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4315) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (273, 1897, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7263) [M::mem_pestat] mean and std.dev: (1728.14, 1577.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9593) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (446, 1696, 2687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7169) [M::mem_pestat] mean and std.dev: (1723.67, 1626.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9410) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1201.255 CPU sec, 53.029 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1029415, 36, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (303, 333, 608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1218) [M::mem_pestat] mean and std.dev: (399.18, 177.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (633, 2585, 2611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6567) [M::mem_pestat] mean and std.dev: (1792.64, 1183.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8545) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (703, 2547, 5626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15472) [M::mem_pestat] mean and std.dev: (3352.53, 2699.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20395) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1206.075 CPU sec, 64.988 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 1028611, 40, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (303, 352, 686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1452) [M::mem_pestat] mean and std.dev: (431.30, 235.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1835) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (837, 2514, 3490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8796) [M::mem_pestat] mean and std.dev: (2601.25, 2337.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11949) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1206.521 CPU sec, 53.100 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1029267, 49, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (304, 349, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (78, 643) [M::mem_pestat] mean and std.dev: (336.68, 97.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1112, 2590, 2609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5603) [M::mem_pestat] mean and std.dev: (1989.66, 1285.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7130) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (565, 1131, 2182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5416) [M::mem_pestat] mean and std.dev: (914.60, 587.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7033) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1251.949 CPU sec, 74.648 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1029252, 44, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (341, 383, 2308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6242) [M::mem_pestat] mean and std.dev: (978.81, 1294.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8209) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (400, 1880, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7015) [M::mem_pestat] mean and std.dev: (1503.38, 1204.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9220) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1217.017 CPU sec, 55.425 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1028940, 52, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 363, 2083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5617) [M::mem_pestat] mean and std.dev: (973.09, 1321.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (636, 2576, 2613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6567) [M::mem_pestat] mean and std.dev: (1881.12, 1397.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8544) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (481, 1007, 2024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5110) [M::mem_pestat] mean and std.dev: (1200.55, 869.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6653) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1197.468 CPU sec, 64.548 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1029303, 52, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 375, 954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2212) [M::mem_pestat] mean and std.dev: (537.95, 364.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2841) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1394, 2591, 2806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5630) [M::mem_pestat] mean and std.dev: (1950.87, 1151.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7042) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (393, 632, 1283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3063) [M::mem_pestat] mean and std.dev: (756.50, 457.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3953) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1200.124 CPU sec, 52.837 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1028716, 47, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 314, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (288.81, 110.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (676, 2588, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6469) [M::mem_pestat] mean and std.dev: (1920.87, 1227.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8400) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (794, 1212, 2013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4451) [M::mem_pestat] mean and std.dev: (1326.57, 789.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5670) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1214.352 CPU sec, 68.614 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1029769, 32, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 391, 1750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4592) [M::mem_pestat] mean and std.dev: (963.71, 941.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6013) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1208, 2588, 2817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6035) [M::mem_pestat] mean and std.dev: (2015.47, 1330.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7644) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1195.170 CPU sec, 52.777 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 1030004, 47, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (315, 401, 1425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3645) [M::mem_pestat] mean and std.dev: (777.33, 914.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (269, 1897, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7280) [M::mem_pestat] mean and std.dev: (1611.50, 1355.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9617) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (912, 1053, 3053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7335) [M::mem_pestat] mean and std.dev: (1971.00, 1824.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9476) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1253.997 CPU sec, 75.434 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1029015, 46, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (313, 329, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (321.72, 130.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1476, 2595, 3228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6732) [M::mem_pestat] mean and std.dev: (2197.66, 1398.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8484) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (925, 1389, 2527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5731) [M::mem_pestat] mean and std.dev: (1523.75, 926.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7333) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1216.612 CPU sec, 55.626 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1029196, 52, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (322, 576, 2321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6319) [M::mem_pestat] mean and std.dev: (990.13, 1057.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8318) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (386, 2567, 2599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7025) [M::mem_pestat] mean and std.dev: (1701.98, 1186.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9238) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (363, 756, 1990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5244) [M::mem_pestat] mean and std.dev: (955.73, 863.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6871) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1200.615 CPU sec, 64.248 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1028299, 39, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (328, 374, 918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2098) [M::mem_pestat] mean and std.dev: (685.70, 562.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (118, 1302, 2595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7549) [M::mem_pestat] mean and std.dev: (1473.69, 1617.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10026) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (409, 1480, 2203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5791) [M::mem_pestat] mean and std.dev: (1361.08, 1017.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7585) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1194.276 CPU sec, 52.598 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1029143, 54, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (313, 367, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069) [M::mem_pestat] mean and std.dev: (399.23, 194.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (367, 2577, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7087) [M::mem_pestat] mean and std.dev: (1764.54, 1440.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9327) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (686, 893, 1584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3380) [M::mem_pestat] mean and std.dev: (969.60, 457.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4278) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1210.055 CPU sec, 64.816 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1028595, 52, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (300, 380, 1301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3303) [M::mem_pestat] mean and std.dev: (570.40, 459.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.38) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (245, 1624, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7325) [M::mem_pestat] mean and std.dev: (1763.47, 1706.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9685) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (568, 1102, 1803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4273) [M::mem_pestat] mean and std.dev: (1490.90, 1208.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6324) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1206.360 CPU sec, 53.351 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1030276, 38, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 343, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027) [M::mem_pestat] mean and std.dev: (360.00, 180.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1272) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (309, 2594, 2615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7227) [M::mem_pestat] mean and std.dev: (1717.26, 1234.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9533) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1191.977 CPU sec, 52.539 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1028641, 41, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (304, 429, 1284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3244) [M::mem_pestat] mean and std.dev: (729.85, 659.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4224) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1085, 2583, 2604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5642) [M::mem_pestat] mean and std.dev: (1824.58, 1205.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7161) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1293, 2427, 3006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6432) [M::mem_pestat] mean and std.dev: (2177.73, 1508.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8213) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1263.071 CPU sec, 78.019 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1028720, 42, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (342, 461, 1087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2577) [M::mem_pestat] mean and std.dev: (686.17, 470.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3322) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1132, 2583, 2613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5575) [M::mem_pestat] mean and std.dev: (1906.46, 1223.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7056) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (437, 891, 2251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5879) [M::mem_pestat] mean and std.dev: (856.77, 622.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7693) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1214.493 CPU sec, 54.920 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1029124, 46, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 394, 871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1989) [M::mem_pestat] mean and std.dev: (640.88, 529.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (238, 1662, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7324) [M::mem_pestat] mean and std.dev: (1380.59, 1080.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9686) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (454, 919, 1829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4579) [M::mem_pestat] mean and std.dev: (1039.42, 708.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5954) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1213.672 CPU sec, 65.797 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1029389, 42, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 658, 2882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7988) [M::mem_pestat] mean and std.dev: (1926.17, 2363.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (272, 1937, 2656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7424) [M::mem_pestat] mean and std.dev: (1713.11, 1534.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9808) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1201.646 CPU sec, 52.879 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1029195, 59, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 357, 667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1391) [M::mem_pestat] mean and std.dev: (436.67, 327.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (661, 2014, 2611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6511) [M::mem_pestat] mean and std.dev: (1671.21, 1254.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8461) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (393, 1505, 2231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5907) [M::mem_pestat] mean and std.dev: (1638.46, 1572.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7928) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1207.432 CPU sec, 66.751 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1030157, 63, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 362, 765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1635) [M::mem_pestat] mean and std.dev: (442.07, 348.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2070) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (644, 2492, 2602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6518) [M::mem_pestat] mean and std.dev: (1911.98, 1495.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8476) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1194.572 CPU sec, 52.567 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1028114, 40, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (280, 337, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027) [M::mem_pestat] mean and std.dev: (295.94, 100.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1276) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1052, 1956, 3688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8960) [M::mem_pestat] mean and std.dev: (2826.68, 2647.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13415) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1270.306 CPU sec, 75.837 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1029498, 50, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (320, 372, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1067) [M::mem_pestat] mean and std.dev: (379.71, 157.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1316) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (318, 2585, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7185) [M::mem_pestat] mean and std.dev: (1722.41, 1277.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9474) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (501, 933, 2038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5112) [M::mem_pestat] mean and std.dev: (965.20, 673.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6649) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1215.250 CPU sec, 55.503 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1029489, 52, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (285, 424, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (311.23, 134.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (318, 2583, 2599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7161) [M::mem_pestat] mean and std.dev: (1862.36, 1394.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9442) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (486, 1364, 2277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5859) [M::mem_pestat] mean and std.dev: (1741.83, 1542.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7912) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1205.329 CPU sec, 65.084 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1028690, 46, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (339, 357, 1727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4503) [M::mem_pestat] mean and std.dev: (698.76, 939.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5891) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (355, 2591, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7108) [M::mem_pestat] mean and std.dev: (1667.88, 1304.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9359) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (355, 399, 790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1660) [M::mem_pestat] mean and std.dev: (510.25, 262.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1204.706 CPU sec, 53.173 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 1029553, 49, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (315, 345, 1074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2592) [M::mem_pestat] mean and std.dev: (593.82, 503.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (286, 2256, 4292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12304) [M::mem_pestat] mean and std.dev: (2816.33, 2788.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16310) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1213.290 CPU sec, 66.873 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1028783, 59, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (374, 758, 6175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17777) [M::mem_pestat] mean and std.dev: (2346.88, 2989.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (438, 2585, 2608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6948) [M::mem_pestat] mean and std.dev: (1852.29, 1213.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9118) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (514, 1064, 1349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3019) [M::mem_pestat] mean and std.dev: (1041.42, 753.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4054) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1199.095 CPU sec, 52.911 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 1028876, 45, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (297, 369, 832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1902) [M::mem_pestat] mean and std.dev: (474.81, 292.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (193, 1744, 2602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7420) [M::mem_pestat] mean and std.dev: (1734.10, 1735.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9829) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (701, 918, 1295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2483) [M::mem_pestat] mean and std.dev: (997.44, 547.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3189) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1269.321 CPU sec, 75.416 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1028350, 36, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (335, 372, 523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (375.67, 79.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1087) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (269, 2579, 2608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7286) [M::mem_pestat] mean and std.dev: (1706.39, 1507.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9625) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1219.671 CPU sec, 55.853 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1028896, 40, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (328, 351, 967) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2245) [M::mem_pestat] mean and std.dev: (430.88, 348.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.50, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (420, 1917, 2592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6936) [M::mem_pestat] mean and std.dev: (1570.79, 1031.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9108) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1212.273 CPU sec, 65.240 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1029462, 46, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (323, 364, 2242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6080) [M::mem_pestat] mean and std.dev: (1113.26, 1533.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7999) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (144, 1886, 2921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8475) [M::mem_pestat] mean and std.dev: (1659.30, 1412.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11252) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (786, 937, 1655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3393) [M::mem_pestat] mean and std.dev: (1104.69, 576.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4262) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1207.168 CPU sec, 53.203 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 1029036, 36, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (318, 367, 936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2172) [M::mem_pestat] mean and std.dev: (576.03, 463.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.46, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (338, 1894, 2611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7157) [M::mem_pestat] mean and std.dev: (1707.39, 1490.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9430) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (459, 1223, 2013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5121) [M::mem_pestat] mean and std.dev: (1192.75, 958.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6675) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1205.609 CPU sec, 66.035 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1028767, 49, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 348, 857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1947) [M::mem_pestat] mean and std.dev: (459.11, 409.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.46, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1193, 2593, 2602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5420) [M::mem_pestat] mean and std.dev: (2023.26, 1234.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6962) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (777, 1267, 2037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4557) [M::mem_pestat] mean and std.dev: (1241.83, 558.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5817) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1204.253 CPU sec, 53.033 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1029509, 50, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 343, 696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1428) [M::mem_pestat] mean and std.dev: (331.41, 118.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1794) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (447, 2585, 2609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6933) [M::mem_pestat] mean and std.dev: (2061.98, 1476.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9095) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1266.568 CPU sec, 75.608 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1028679, 57, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (291, 376, 1311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3351) [M::mem_pestat] mean and std.dev: (602.90, 482.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4371) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (290, 1914, 2993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8399) [M::mem_pestat] mean and std.dev: (1947.91, 1884.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11102) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (626, 1192, 2756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7016) [M::mem_pestat] mean and std.dev: (1544.47, 1559.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9146) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1225.131 CPU sec, 55.859 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1029240, 57, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (336, 347, 919) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2085) [M::mem_pestat] mean and std.dev: (498.76, 391.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (417, 2588, 2612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7002) [M::mem_pestat] mean and std.dev: (1887.49, 1388.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9197) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1208.495 CPU sec, 64.569 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1028835, 56, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 369, 641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1265) [M::mem_pestat] mean and std.dev: (387.12, 154.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (534, 2584, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6747) [M::mem_pestat] mean and std.dev: (2027.27, 1562.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8818) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1206.876 CPU sec, 53.302 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1029515, 53, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 387, 1840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4866) [M::mem_pestat] mean and std.dev: (796.83, 839.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.50, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (412, 2576, 2611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7009) [M::mem_pestat] mean and std.dev: (1808.71, 1189.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9208) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (408, 1739, 1952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5040) [M::mem_pestat] mean and std.dev: (1462.58, 1338.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6815) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1207.938 CPU sec, 68.228 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1029444, 29, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (290, 383, 1754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4682) [M::mem_pestat] mean and std.dev: (965.92, 1089.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6146) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1483, 2580, 3005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6049) [M::mem_pestat] mean and std.dev: (2224.92, 1470.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8106) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1197.538 CPU sec, 52.787 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1029284, 53, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (294, 379, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (48, 663) [M::mem_pestat] mean and std.dev: (318.15, 78.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.50, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (416, 2591, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6986) [M::mem_pestat] mean and std.dev: (1793.90, 1152.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9176) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1261.205 CPU sec, 75.700 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1029676, 52, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 343, 658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1376) [M::mem_pestat] mean and std.dev: (423.53, 284.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (429, 1898, 2604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6954) [M::mem_pestat] mean and std.dev: (1752.64, 1404.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9129) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (365, 915, 2513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6809) [M::mem_pestat] mean and std.dev: (1497.31, 1596.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8957) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1216.343 CPU sec, 55.276 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1029355, 45, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (323, 381, 2145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5789) [M::mem_pestat] mean and std.dev: (1084.79, 1356.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1394, 2589, 2599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5009) [M::mem_pestat] mean and std.dev: (2006.17, 1276.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7110) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (382, 919, 1835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4741) [M::mem_pestat] mean and std.dev: (1116.33, 1037.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6194) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1216.589 CPU sec, 66.986 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1029176, 45, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 383, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1630) [M::mem_pestat] mean and std.dev: (479.74, 310.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2062) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.50, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1176, 2584, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5466) [M::mem_pestat] mean and std.dev: (1831.08, 1075.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6896) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1197.363 CPU sec, 52.752 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 1029578, 49, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (343, 680, 1785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4669) [M::mem_pestat] mean and std.dev: (931.17, 871.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6111) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (838, 2590, 2969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7231) [M::mem_pestat] mean and std.dev: (2026.98, 1421.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9362) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (640, 1270, 4735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12925) [M::mem_pestat] mean and std.dev: (2505.80, 2057.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17020) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1210.593 CPU sec, 65.109 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1029564, 53, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (323, 360, 1178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2888) [M::mem_pestat] mean and std.dev: (608.56, 489.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1273, 2592, 2613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5293) [M::mem_pestat] mean and std.dev: (2023.80, 1237.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6973) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1203.637 CPU sec, 53.124 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1029510, 50, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 344, 607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1275) [M::mem_pestat] mean and std.dev: (316.53, 165.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (450, 2593, 3485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9555) [M::mem_pestat] mean and std.dev: (3079.94, 2800.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14281) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1037, 1236, 2703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6035) [M::mem_pestat] mean and std.dev: (1615.69, 1122.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7701) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1269.050 CPU sec, 76.086 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1029280, 51, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 359, 922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2134) [M::mem_pestat] mean and std.dev: (577.05, 473.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (318, 1870, 2594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7146) [M::mem_pestat] mean and std.dev: (1546.10, 1082.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9422) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (804, 1440, 2376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5520) [M::mem_pestat] mean and std.dev: (1565.00, 916.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7092) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1225.978 CPU sec, 56.017 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1029794, 46, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (285, 347, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1140) [M::mem_pestat] mean and std.dev: (357.06, 193.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.49) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (256, 2346, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7306) [M::mem_pestat] mean and std.dev: (1738.40, 1569.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9656) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (546, 1114, 1727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4089) [M::mem_pestat] mean and std.dev: (991.30, 572.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5270) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1223.386 CPU sec, 67.063 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1029021, 46, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (319, 337, 1831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4855) [M::mem_pestat] mean and std.dev: (946.65, 949.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6367) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (422, 2585, 3378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9290) [M::mem_pestat] mean and std.dev: (2633.46, 2285.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12246) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1226.301 CPU sec, 54.283 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1029143, 54, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (336, 380, 2226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6006) [M::mem_pestat] mean and std.dev: (1096.95, 1363.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (525, 2583, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6768) [M::mem_pestat] mean and std.dev: (1680.60, 1194.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8849) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1218.367 CPU sec, 64.742 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1030009, 45, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (341, 374, 1183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2867) [M::mem_pestat] mean and std.dev: (598.47, 546.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1471, 2579, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4873) [M::mem_pestat] mean and std.dev: (1859.15, 1073.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6152) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1214.679 CPU sec, 53.499 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1029315, 48, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (310, 404, 1501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3883) [M::mem_pestat] mean and std.dev: (744.90, 638.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5074) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.49) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (216, 1902, 2613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7407) [M::mem_pestat] mean and std.dev: (1686.98, 1674.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9804) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1264.099 CPU sec, 75.353 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1029368, 51, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (326, 367, 1734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4550) [M::mem_pestat] mean and std.dev: (878.88, 771.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5958) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1365, 2589, 3337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7281) [M::mem_pestat] mean and std.dev: (2141.70, 1454.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9253) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (923, 1331, 1966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4052) [M::mem_pestat] mean and std.dev: (1271.67, 593.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1222.299 CPU sec, 55.885 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 1029570, 43, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (307, 376, 1433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3685) [M::mem_pestat] mean and std.dev: (846.87, 856.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (696, 2582, 2604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6420) [M::mem_pestat] mean and std.dev: (1964.49, 1297.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8328) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1208.088 CPU sec, 65.493 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1028352, 48, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 403, 973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2261) [M::mem_pestat] mean and std.dev: (693.12, 621.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3178) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (638, 2584, 2602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6530) [M::mem_pestat] mean and std.dev: (1906.34, 1407.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8494) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1205.607 CPU sec, 53.193 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1029176, 52, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (332, 357, 741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1559) [M::mem_pestat] mean and std.dev: (468.14, 372.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1968) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1878, 2596, 2622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (390, 4110) [M::mem_pestat] mean and std.dev: (2411.91, 460.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4854) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (585, 1169, 2128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5214) [M::mem_pestat] mean and std.dev: (1000.33, 748.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6757) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1217.245 CPU sec, 66.028 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 1029465, 55, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 365, 873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1991) [M::mem_pestat] mean and std.dev: (470.58, 293.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1412, 2594, 4038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9290) [M::mem_pestat] mean and std.dev: (3123.35, 2609.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13561) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1055, 1432, 2704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6002) [M::mem_pestat] mean and std.dev: (2005.64, 1649.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8604) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1215.434 CPU sec, 53.606 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 1029073, 57, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 655, 2777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7801) [M::mem_pestat] mean and std.dev: (1438.23, 1704.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10313) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (264, 2567, 2716) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7620) [M::mem_pestat] mean and std.dev: (1919.65, 1700.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10072) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (548, 1416, 2611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6737) [M::mem_pestat] mean and std.dev: (1760.86, 1633.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8800) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1204.787 CPU sec, 53.949 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1028575, 42, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 372, 1228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3008) [M::mem_pestat] mean and std.dev: (726.55, 688.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3898) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1484, 2590, 2941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5855) [M::mem_pestat] mean and std.dev: (2120.19, 1230.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7312) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (406, 1354, 2189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5755) [M::mem_pestat] mean and std.dev: (1503.69, 1272.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7538) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1271.324 CPU sec, 76.458 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1029001, 50, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (310, 412, 1468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3784) [M::mem_pestat] mean and std.dev: (778.53, 801.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4942) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1125, 2585, 2857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6321) [M::mem_pestat] mean and std.dev: (2003.62, 1269.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8053) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (383, 1157, 2479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6671) [M::mem_pestat] mean and std.dev: (1647.00, 1548.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8767) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1227.868 CPU sec, 56.061 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1029552, 53, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (332, 624, 1558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4010) [M::mem_pestat] mean and std.dev: (761.20, 602.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5236) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1281, 2587, 2630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5328) [M::mem_pestat] mean and std.dev: (1966.00, 1279.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7085) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1215.984 CPU sec, 67.309 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1029461, 53, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (328, 410, 1299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3241) [M::mem_pestat] mean and std.dev: (768.19, 685.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4212) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (479, 2584, 2598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6836) [M::mem_pestat] mean and std.dev: (1846.94, 1243.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8955) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1218.116 CPU sec, 53.689 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 1029159, 47, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 365, 1084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2624) [M::mem_pestat] mean and std.dev: (622.00, 579.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (268, 2581, 2611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7297) [M::mem_pestat] mean and std.dev: (1738.71, 1363.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9640) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (934, 1212, 3144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7564) [M::mem_pestat] mean and std.dev: (2173.50, 1946.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9958) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1208.384 CPU sec, 65.246 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1028889, 37, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 350, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (15, 770) [M::mem_pestat] mean and std.dev: (375.41, 145.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 955) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (535, 2600, 3472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9346) [M::mem_pestat] mean and std.dev: (2673.62, 2355.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12283) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (471, 993, 1733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4257) [M::mem_pestat] mean and std.dev: (1156.36, 720.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5519) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1203.639 CPU sec, 53.094 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1028636, 48, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (279, 357, 941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2265) [M::mem_pestat] mean and std.dev: (495.27, 505.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2927) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (458, 2458, 2653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7043) [M::mem_pestat] mean and std.dev: (1927.14, 1607.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9238) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (704, 1022, 2419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5849) [M::mem_pestat] mean and std.dev: (1420.62, 1059.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7564) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1264.649 CPU sec, 75.548 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1028889, 52, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (275, 378, 730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1640) [M::mem_pestat] mean and std.dev: (392.79, 230.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2095) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1151, 2593, 3005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6713) [M::mem_pestat] mean and std.dev: (2134.74, 1421.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8567) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (331, 732, 1670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4348) [M::mem_pestat] mean and std.dev: (925.00, 931.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5687) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1225.090 CPU sec, 55.925 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1028470, 34, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (288, 353, 1313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3363) [M::mem_pestat] mean and std.dev: (672.70, 779.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (277, 2594, 2652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7402) [M::mem_pestat] mean and std.dev: (2006.59, 1514.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9777) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (573, 1045, 4500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12354) [M::mem_pestat] mean and std.dev: (1975.18, 1738.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16281) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1231.785 CPU sec, 64.761 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1029950, 68, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (308, 388, 821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1847) [M::mem_pestat] mean and std.dev: (489.88, 280.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (282, 2585, 2610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7266) [M::mem_pestat] mean and std.dev: (1847.43, 1584.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9594) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (797, 996, 1960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4286) [M::mem_pestat] mean and std.dev: (1194.00, 721.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5449) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1208.728 CPU sec, 53.242 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1029701, 45, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 358, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (121, 566) [M::mem_pestat] mean and std.dev: (303.07, 86.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (934, 2407, 3317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8083) [M::mem_pestat] mean and std.dev: (1946.37, 1610.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10466) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (676, 947, 1296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2536) [M::mem_pestat] mean and std.dev: (957.40, 455.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3156) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1213.644 CPU sec, 61.453 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1028641, 51, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (332, 353, 638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1250) [M::mem_pestat] mean and std.dev: (411.58, 202.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (432, 2551, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6945) [M::mem_pestat] mean and std.dev: (1695.71, 1310.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9116) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1211.025 CPU sec, 53.333 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 1028902, 54, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (308, 357, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (80, 650) [M::mem_pestat] mean and std.dev: (314.59, 89.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (260, 1909, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7298) [M::mem_pestat] mean and std.dev: (1610.65, 1333.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9644) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (815, 2149, 4749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12617) [M::mem_pestat] mean and std.dev: (2601.10, 2088.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16551) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1263.138 CPU sec, 75.302 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1030225, 50, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (328, 577, 4406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12562) [M::mem_pestat] mean and std.dev: (2202.56, 3181.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (276, 2582, 2602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7254) [M::mem_pestat] mean and std.dev: (1863.55, 1558.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9580) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1227.594 CPU sec, 56.098 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 1029423, 59, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (282, 380, 1411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3669) [M::mem_pestat] mean and std.dev: (713.18, 614.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1367, 2577, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5084) [M::mem_pestat] mean and std.dev: (1774.38, 1167.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6443) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (377, 1106, 1977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5177) [M::mem_pestat] mean and std.dev: (1202.16, 1202.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6777) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1220.626 CPU sec, 66.602 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1030034, 50, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (295, 353, 1240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3130) [M::mem_pestat] mean and std.dev: (727.91, 676.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (277, 1438, 2597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7237) [M::mem_pestat] mean and std.dev: (1576.59, 1494.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9557) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1000, 1186, 1940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3820) [M::mem_pestat] mean and std.dev: (1417.73, 992.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5388) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1200.376 CPU sec, 53.177 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1029283, 44, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (323, 356, 1853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4913) [M::mem_pestat] mean and std.dev: (779.04, 713.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6443) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (422, 2563, 2610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6986) [M::mem_pestat] mean and std.dev: (1619.55, 1106.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9174) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1222.978 CPU sec, 69.555 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1028653, 47, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 349, 945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2167) [M::mem_pestat] mean and std.dev: (491.59, 417.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1679, 2593, 2630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4532) [M::mem_pestat] mean and std.dev: (1991.00, 1100.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6393) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1217.166 CPU sec, 53.873 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 1029316, 58, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (315, 378, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1884) [M::mem_pestat] mean and std.dev: (377.27, 205.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1048, 2584, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5722) [M::mem_pestat] mean and std.dev: (1967.70, 1164.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7280) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (437, 835, 1585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3881) [M::mem_pestat] mean and std.dev: (1021.77, 779.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5029) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1278.548 CPU sec, 76.290 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1029150, 46, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (268, 362, 1398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3658) [M::mem_pestat] mean and std.dev: (873.17, 993.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1405, 2594, 2857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5761) [M::mem_pestat] mean and std.dev: (2183.37, 1332.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1206, 1623, 2345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4623) [M::mem_pestat] mean and std.dev: (1510.10, 667.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5762) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1213.774 CPU sec, 55.736 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1029299, 49, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (342, 667, 1129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2703) [M::mem_pestat] mean and std.dev: (719.41, 479.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (251, 1884, 2907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8219) [M::mem_pestat] mean and std.dev: (1601.77, 1540.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10875) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (576, 1089, 4684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12900) [M::mem_pestat] mean and std.dev: (2810.64, 2775.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17008) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1242.000 CPU sec, 68.246 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1028997, 53, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (266, 352, 715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1613) [M::mem_pestat] mean and std.dev: (382.58, 250.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2062) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (251, 2144, 2633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7397) [M::mem_pestat] mean and std.dev: (1676.44, 1341.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9779) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1215.209 CPU sec, 53.621 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1029239, 52, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 370, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (50, 735) [M::mem_pestat] mean and std.dev: (351.22, 142.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 921) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1132, 2582, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5551) [M::mem_pestat] mean and std.dev: (1865.13, 1081.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7024) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1216.393 CPU sec, 67.241 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1028703, 46, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (347, 382, 2093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5585) [M::mem_pestat] mean and std.dev: (902.95, 900.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7331) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1391, 2591, 2609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5045) [M::mem_pestat] mean and std.dev: (1903.87, 1115.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6364) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (432, 559, 1887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4797) [M::mem_pestat] mean and std.dev: (929.00, 835.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6252) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1216.403 CPU sec, 53.613 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1028557, 50, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (355, 542, 1086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2548) [M::mem_pestat] mean and std.dev: (703.75, 575.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3279) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1956, 2596, 3837) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7599) [M::mem_pestat] mean and std.dev: (2544.16, 1613.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9480) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (809, 2839, 6746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18620) [M::mem_pestat] mean and std.dev: (3507.54, 2980.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 24557) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1257.621 CPU sec, 75.398 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1029837, 58, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (322, 365, 1690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4426) [M::mem_pestat] mean and std.dev: (545.06, 412.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5794) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1133, 2593, 2614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5576) [M::mem_pestat] mean and std.dev: (1895.28, 1122.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7057) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (446, 1771, 2833) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7607) [M::mem_pestat] mean and std.dev: (1465.00, 1202.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9994) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1234.043 CPU sec, 56.642 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 1029229, 39, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 359, 1178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2900) [M::mem_pestat] mean and std.dev: (531.15, 397.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (311, 1484, 2604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7190) [M::mem_pestat] mean and std.dev: (1415.49, 1056.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9483) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1231.619 CPU sec, 68.075 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1029124, 39, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 338, 719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1525) [M::mem_pestat] mean and std.dev: (451.13, 295.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1928) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1657, 2598, 3888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8350) [M::mem_pestat] mean and std.dev: (2500.49, 1809.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10581) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1486, 2416, 4681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11071) [M::mem_pestat] mean and std.dev: (2975.36, 2305.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14266) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1220.261 CPU sec, 53.846 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1029587, 46, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (267, 359, 988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2430) [M::mem_pestat] mean and std.dev: (539.04, 379.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3151) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1871, 2591, 2861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4841) [M::mem_pestat] mean and std.dev: (2060.75, 1078.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6375) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1234.998 CPU sec, 70.128 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1029901, 53, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 354, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (34, 734) [M::mem_pestat] mean and std.dev: (329.86, 58.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (289, 1908, 2604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7234) [M::mem_pestat] mean and std.dev: (1877.64, 1431.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9549) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1162, 1366, 2777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6007) [M::mem_pestat] mean and std.dev: (1656.70, 1067.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7622) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1218.665 CPU sec, 53.847 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 1028683, 52, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (290, 349, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1031) [M::mem_pestat] mean and std.dev: (334.88, 118.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1278) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1047, 2585, 3354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7968) [M::mem_pestat] mean and std.dev: (2202.81, 1563.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10275) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (546, 876, 1796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4296) [M::mem_pestat] mean and std.dev: (1155.45, 787.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5546) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1263.277 CPU sec, 76.036 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1027869, 59, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (257, 388, 846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2024) [M::mem_pestat] mean and std.dev: (457.27, 356.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (266, 1894, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7283) [M::mem_pestat] mean and std.dev: (1579.62, 1177.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9622) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (362, 904, 2913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8015) [M::mem_pestat] mean and std.dev: (1658.54, 1877.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10566) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1235.023 CPU sec, 56.520 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1028733, 48, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 366, 1087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2633) [M::mem_pestat] mean and std.dev: (624.31, 596.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3406) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (447, 2588, 2645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7041) [M::mem_pestat] mean and std.dev: (1931.30, 1503.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9239) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1212.594 CPU sec, 66.039 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1028746, 38, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (335, 751, 2066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5528) [M::mem_pestat] mean and std.dev: (1106.40, 998.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7259) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1228, 2591, 2604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5356) [M::mem_pestat] mean and std.dev: (1889.92, 1105.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6732) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (405, 796, 3068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8394) [M::mem_pestat] mean and std.dev: (1651.79, 1813.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11057) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1208.136 CPU sec, 53.223 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1029066, 48, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (344, 382, 1480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3752) [M::mem_pestat] mean and std.dev: (772.38, 809.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4888) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1089, 2227, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5631) [M::mem_pestat] mean and std.dev: (1733.72, 1119.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7145) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1216.490 CPU sec, 66.772 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 1028839, 55, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (311, 357, 1107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2699) [M::mem_pestat] mean and std.dev: (605.37, 455.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (287, 1888, 2596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7214) [M::mem_pestat] mean and std.dev: (1601.89, 1233.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9523) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1631, 3030, 3438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7052) [M::mem_pestat] mean and std.dev: (2535.00, 1600.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8938) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1204.838 CPU sec, 53.034 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1029307, 42, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (276, 314, 695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1533) [M::mem_pestat] mean and std.dev: (351.40, 295.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (274, 2565, 2595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7237) [M::mem_pestat] mean and std.dev: (1811.20, 1469.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9558) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (299, 411, 983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2351) [M::mem_pestat] mean and std.dev: (528.93, 448.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3035) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1199.640 CPU sec, 52.901 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1029751, 48, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 358, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (238, 478) [M::mem_pestat] mean and std.dev: (346.81, 33.34) [M::mem_pestat] low and high boundaries for proper pairs: (190, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (214, 2595, 3244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9304) [M::mem_pestat] mean and std.dev: (2514.04, 2359.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12334) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (914, 2175, 3033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7271) [M::mem_pestat] mean and std.dev: (2227.31, 1738.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9390) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1251.848 CPU sec, 77.980 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1029798, 61, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (337, 366, 1062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2512) [M::mem_pestat] mean and std.dev: (645.38, 546.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3237) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (423, 2592, 3108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8478) [M::mem_pestat] mean and std.dev: (2135.26, 1689.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11163) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (675, 1164, 4469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12057) [M::mem_pestat] mean and std.dev: (2396.23, 2607.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15851) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1223.444 CPU sec, 54.762 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 1029401, 44, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (309, 331, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (189, 489) [M::mem_pestat] mean and std.dev: (330.75, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (129, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (427, 2593, 2616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6994) [M::mem_pestat] mean and std.dev: (2107.69, 1583.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9183) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (639, 1448, 1733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3921) [M::mem_pestat] mean and std.dev: (1190.22, 605.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5015) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1221.475 CPU sec, 68.424 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (38, 1029360, 50, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 357, 939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2263) [M::mem_pestat] mean and std.dev: (423.78, 325.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2925) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (324, 2546, 2609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7179) [M::mem_pestat] mean and std.dev: (1746.84, 1386.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9464) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (447, 793, 1299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3003) [M::mem_pestat] mean and std.dev: (669.73, 328.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3855) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1206.177 CPU sec, 53.237 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1028992, 40, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (328, 404, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973) [M::mem_pestat] mean and std.dev: (397.87, 117.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1188) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (338, 1888, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7124) [M::mem_pestat] mean and std.dev: (1583.50, 1226.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9386) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (359, 1479, 2054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5444) [M::mem_pestat] mean and std.dev: (1662.00, 1491.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7628) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1233.374 CPU sec, 69.320 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1029158, 60, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (331, 377, 1092) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2614) [M::mem_pestat] mean and std.dev: (402.88, 218.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (131, 1641, 2601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7541) [M::mem_pestat] mean and std.dev: (1501.68, 1212.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10011) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (490, 1089, 1971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4933) [M::mem_pestat] mean and std.dev: (1250.31, 854.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6414) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1214.167 CPU sec, 53.488 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 1029525, 47, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (295, 361, 1186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2968) [M::mem_pestat] mean and std.dev: (564.61, 499.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (699, 2583, 2604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6414) [M::mem_pestat] mean and std.dev: (1906.04, 1269.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8319) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (388, 1946, 2192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5800) [M::mem_pestat] mean and std.dev: (1310.67, 828.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7604) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1263.898 CPU sec, 75.314 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1029347, 40, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 327, 904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2150) [M::mem_pestat] mean and std.dev: (528.79, 419.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (288, 2583, 2610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7254) [M::mem_pestat] mean and std.dev: (1792.26, 1301.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9576) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1229.424 CPU sec, 55.970 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1029687, 64, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 378, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1772) [M::mem_pestat] mean and std.dev: (455.33, 350.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2261) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (654, 1950, 2609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6519) [M::mem_pestat] mean and std.dev: (1601.80, 1117.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8474) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (679, 1325, 1980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4582) [M::mem_pestat] mean and std.dev: (1377.10, 838.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5883) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1213.694 CPU sec, 65.863 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1029368, 48, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 365, 1251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3205) [M::mem_pestat] mean and std.dev: (663.91, 819.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4182) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (354, 2570, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7113) [M::mem_pestat] mean and std.dev: (1676.18, 1141.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9366) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1220.588 CPU sec, 53.875 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 1029435, 47, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 374, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 925) [M::mem_pestat] mean and std.dev: (351.43, 56.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1004, 2589, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5807) [M::mem_pestat] mean and std.dev: (1849.51, 1180.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7408) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (357, 879, 2244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6018) [M::mem_pestat] mean and std.dev: (1520.10, 1758.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8554) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1228.750 CPU sec, 69.812 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1029289, 47, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (288, 329, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (60, 630) [M::mem_pestat] mean and std.dev: (287.75, 86.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (608, 1918, 2845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7319) [M::mem_pestat] mean and std.dev: (1698.60, 1221.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9556) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (342, 1450, 2372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6432) [M::mem_pestat] mean and std.dev: (1511.00, 1330.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8462) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1220.179 CPU sec, 54.004 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1029149, 56, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (321, 352, 1204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2970) [M::mem_pestat] mean and std.dev: (631.95, 653.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.46, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (376, 2583, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7066) [M::mem_pestat] mean and std.dev: (1742.26, 1270.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9296) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (448, 963, 1686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4162) [M::mem_pestat] mean and std.dev: (888.09, 457.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5400) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1269.533 CPU sec, 75.811 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1030012, 38, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 350, 2668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7344) [M::mem_pestat] mean and std.dev: (1185.90, 1743.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1153, 2586, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5512) [M::mem_pestat] mean and std.dev: (1955.51, 1071.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6965) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (724, 1990, 2595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6337) [M::mem_pestat] mean and std.dev: (1629.82, 1031.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8208) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1223.366 CPU sec, 55.890 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1029131, 48, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 356, 824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1844) [M::mem_pestat] mean and std.dev: (524.62, 391.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (624, 2589, 2604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6564) [M::mem_pestat] mean and std.dev: (1752.11, 1116.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8544) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1217.903 CPU sec, 66.432 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1029200, 47, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (306, 357, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002) [M::mem_pestat] mean and std.dev: (321.00, 95.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1234) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (431, 2587, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6959) [M::mem_pestat] mean and std.dev: (1852.02, 1440.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9135) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1201.420 CPU sec, 52.922 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1029080, 48, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (309, 634, 2677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7413) [M::mem_pestat] mean and std.dev: (1726.11, 2220.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (388, 2590, 2950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8074) [M::mem_pestat] mean and std.dev: (1899.67, 1553.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10636) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (854, 1338, 1660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3272) [M::mem_pestat] mean and std.dev: (1192.15, 663.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4078) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1216.745 CPU sec, 70.034 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 1029028, 58, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 455, 1706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4470) [M::mem_pestat] mean and std.dev: (752.88, 617.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (652, 2585, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6514) [M::mem_pestat] mean and std.dev: (1896.94, 1464.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8468) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (349, 672, 3052) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8458) [M::mem_pestat] mean and std.dev: (1379.08, 1284.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11161) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1217.024 CPU sec, 53.874 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1029058, 49, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (331, 371, 842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1864) [M::mem_pestat] mean and std.dev: (510.26, 337.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1327, 2570, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5155) [M::mem_pestat] mean and std.dev: (1883.82, 1080.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6431) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1265.306 CPU sec, 75.468 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 1029398, 44, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 354, 938) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2210) [M::mem_pestat] mean and std.dev: (473.86, 276.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1129, 2582, 2602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5548) [M::mem_pestat] mean and std.dev: (1982.07, 1169.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7021) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (459, 1127, 1490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3552) [M::mem_pestat] mean and std.dev: (994.50, 529.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4583) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1226.451 CPU sec, 56.164 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 1028887, 51, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (320, 364, 953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2219) [M::mem_pestat] mean and std.dev: (457.44, 335.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2852) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.46, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (373, 2587, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7054) [M::mem_pestat] mean and std.dev: (1808.56, 1219.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9281) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (399, 654, 2282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6048) [M::mem_pestat] mean and std.dev: (1490.85, 1336.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7931) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1234.000 CPU sec, 69.220 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1029203, 46, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 353, 1069) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2575) [M::mem_pestat] mean and std.dev: (491.67, 365.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3328) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (285, 2586, 2634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7332) [M::mem_pestat] mean and std.dev: (1978.58, 1531.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9681) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (359, 661, 1504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3794) [M::mem_pestat] mean and std.dev: (751.35, 610.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4939) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1217.657 CPU sec, 53.819 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1028726, 40, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (300, 366, 1081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2643) [M::mem_pestat] mean and std.dev: (608.26, 527.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (279, 2580, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7251) [M::mem_pestat] mean and std.dev: (1690.31, 1324.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9575) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (741, 1162, 1520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3078) [M::mem_pestat] mean and std.dev: (1179.27, 587.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3857) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1219.145 CPU sec, 66.325 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1028893, 50, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 354, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (81, 701) [M::mem_pestat] mean and std.dev: (364.20, 72.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.38) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (404, 2579, 2604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7004) [M::mem_pestat] mean and std.dev: (1708.87, 1511.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9204) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1221.029 CPU sec, 54.031 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1029593, 55, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (333, 376, 1675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4359) [M::mem_pestat] mean and std.dev: (720.52, 734.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (688, 2584, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6439) [M::mem_pestat] mean and std.dev: (1916.06, 1397.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8356) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1108, 2833, 3208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7408) [M::mem_pestat] mean and std.dev: (2418.89, 1856.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9846) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1265.693 CPU sec, 75.327 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1028482, 45, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (320, 368, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (18, 773) [M::mem_pestat] mean and std.dev: (347.89, 76.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (541, 2588, 2808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7342) [M::mem_pestat] mean and std.dev: (2179.95, 1667.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9609) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (549, 1093, 2519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6459) [M::mem_pestat] mean and std.dev: (1793.38, 1703.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8608) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1232.121 CPU sec, 56.175 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1029274, 45, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 357, 402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (138, 578) [M::mem_pestat] mean and std.dev: (350.33, 68.19) [M::mem_pestat] low and high boundaries for proper pairs: (50, 666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (258, 2573, 2611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7317) [M::mem_pestat] mean and std.dev: (1724.60, 1366.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9670) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1213.130 CPU sec, 64.930 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1029012, 63, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 459, 2147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5787) [M::mem_pestat] mean and std.dev: (1146.27, 1105.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (266, 1871, 2601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7271) [M::mem_pestat] mean and std.dev: (1639.83, 1488.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9606) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1205.031 CPU sec, 53.048 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1028654, 38, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (297, 332, 988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2370) [M::mem_pestat] mean and std.dev: (543.60, 521.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (388, 2599, 4824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13696) [M::mem_pestat] mean and std.dev: (3168.79, 2922.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18132) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1217.399 CPU sec, 66.455 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 1029030, 45, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (298, 334, 898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2098) [M::mem_pestat] mean and std.dev: (475.38, 374.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (331, 2584, 2615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7183) [M::mem_pestat] mean and std.dev: (1966.54, 1708.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9467) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (751, 1063, 3195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8083) [M::mem_pestat] mean and std.dev: (1514.45, 1232.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1204.446 CPU sec, 53.154 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1029750, 42, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (298, 325, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (24, 709) [M::mem_pestat] mean and std.dev: (341.85, 106.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (608, 2586, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6593) [M::mem_pestat] mean and std.dev: (1887.49, 1362.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8588) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (382, 1003, 3083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8485) [M::mem_pestat] mean and std.dev: (1624.30, 1436.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11186) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1277.189 CPU sec, 76.538 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1028567, 53, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (355, 377, 1824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4762) [M::mem_pestat] mean and std.dev: (874.22, 882.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (408, 2593, 2848) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7728) [M::mem_pestat] mean and std.dev: (2140.61, 1604.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10168) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1211.062 CPU sec, 55.272 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1028788, 50, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (295, 358, 645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1345) [M::mem_pestat] mean and std.dev: (404.43, 203.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (469, 2593, 3515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9607) [M::mem_pestat] mean and std.dev: (2733.98, 2458.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12653) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (989, 1652, 4218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10676) [M::mem_pestat] mean and std.dev: (2047.17, 1596.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13905) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1203.735 CPU sec, 63.452 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1029441, 51, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (307, 949, 1527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3967) [M::mem_pestat] mean and std.dev: (960.00, 922.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5187) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (512, 2588, 2604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6788) [M::mem_pestat] mean and std.dev: (1943.47, 1465.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8880) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (905, 1350, 2058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4364) [M::mem_pestat] mean and std.dev: (1405.40, 1083.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5738) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1209.700 CPU sec, 53.500 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 1029441, 42, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (311, 384, 1669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4385) [M::mem_pestat] mean and std.dev: (924.43, 1017.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (400, 2593, 3647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10141) [M::mem_pestat] mean and std.dev: (2750.95, 2549.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13388) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (482, 1118, 1338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3050) [M::mem_pestat] mean and std.dev: (900.18, 429.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3906) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1199.636 CPU sec, 60.774 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1028198, 60, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (315, 333, 1360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3450) [M::mem_pestat] mean and std.dev: (739.81, 825.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (270, 1911, 2612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7296) [M::mem_pestat] mean and std.dev: (1706.36, 1481.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9638) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (547, 1334, 1728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4090) [M::mem_pestat] mean and std.dev: (1182.29, 640.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5271) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1195.756 CPU sec, 52.638 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1029623, 48, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (311, 489, 1169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2885) [M::mem_pestat] mean and std.dev: (737.37, 690.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.45) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1311, 2578, 2627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5259) [M::mem_pestat] mean and std.dev: (1805.70, 1137.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6575) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1530, 2291, 7894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 20622) [M::mem_pestat] mean and std.dev: (3551.50, 3006.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 26986) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1273.045 CPU sec, 75.869 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1029303, 57, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (307, 369, 1210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3016) [M::mem_pestat] mean and std.dev: (589.88, 570.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3919) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (272, 1352, 2621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7319) [M::mem_pestat] mean and std.dev: (1481.88, 1413.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9668) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1219.358 CPU sec, 55.916 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 1028832, 43, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 364, 1443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3751) [M::mem_pestat] mean and std.dev: (741.97, 836.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4905) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.49) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1167, 2591, 2609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5493) [M::mem_pestat] mean and std.dev: (1948.00, 1251.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6954) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (370, 773, 3213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8899) [M::mem_pestat] mean and std.dev: (1735.88, 1813.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11742) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1211.492 CPU sec, 64.828 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1029525, 41, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (296, 343, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1118) [M::mem_pestat] mean and std.dev: (313.77, 115.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (405, 2405, 2602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6996) [M::mem_pestat] mean and std.dev: (1606.83, 1399.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9193) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1207.522 CPU sec, 53.364 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 1029225, 50, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 426, 2011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5467) [M::mem_pestat] mean and std.dev: (1055.33, 884.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7195) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (316, 1917, 2608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7192) [M::mem_pestat] mean and std.dev: (1655.98, 1318.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9484) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 1180, 4105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11007) [M::mem_pestat] mean and std.dev: (2125.91, 1795.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14458) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1197.447 CPU sec, 57.424 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1029347, 76, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 372, 2820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7826) [M::mem_pestat] mean and std.dev: (1307.30, 1767.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10329) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.50, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1240, 2586, 2604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5332) [M::mem_pestat] mean and std.dev: (2025.56, 1271.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7111) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (537, 827, 2001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4929) [M::mem_pestat] mean and std.dev: (1166.40, 1055.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6393) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1196.647 CPU sec, 52.793 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1028874, 52, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (297, 337, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (57, 657) [M::mem_pestat] mean and std.dev: (314.25, 68.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (388, 2592, 2613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7063) [M::mem_pestat] mean and std.dev: (1606.40, 1212.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9288) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (565, 1152, 1637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3781) [M::mem_pestat] mean and std.dev: (1031.00, 535.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4853) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1200.775 CPU sec, 53.380 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 1029526, 46, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 331, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (71, 596) [M::mem_pestat] mean and std.dev: (313.65, 83.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (114, 1907, 2616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7620) [M::mem_pestat] mean and std.dev: (1783.93, 1695.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10122) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (808, 1147, 2319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5341) [M::mem_pestat] mean and std.dev: (1430.46, 906.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6852) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1247.394 CPU sec, 76.748 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1029339, 41, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (313, 384, 980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2314) [M::mem_pestat] mean and std.dev: (542.28, 401.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (335, 2147, 2610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7160) [M::mem_pestat] mean and std.dev: (1675.73, 1090.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9435) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (382, 826, 1770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4546) [M::mem_pestat] mean and std.dev: (916.56, 602.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5934) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1210.757 CPU sec, 54.942 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 1029098, 42, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (344, 368, 1154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2774) [M::mem_pestat] mean and std.dev: (572.58, 394.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1707, 2596, 3402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6792) [M::mem_pestat] mean and std.dev: (2283.72, 1327.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8487) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1192.045 CPU sec, 58.964 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1028803, 39, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (328, 370, 1544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3976) [M::mem_pestat] mean and std.dev: (544.95, 561.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5192) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1033, 2573, 2610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5764) [M::mem_pestat] mean and std.dev: (1770.00, 1162.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7341) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (435, 1149, 2383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6279) [M::mem_pestat] mean and std.dev: (1460.60, 991.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8227) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1194.005 CPU sec, 52.548 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1029068, 52, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (298, 737, 1413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3643) [M::mem_pestat] mean and std.dev: (683.53, 468.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (547, 2158, 2599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6703) [M::mem_pestat] mean and std.dev: (1899.06, 1428.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8755) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1199.627 CPU sec, 60.035 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1029290, 49, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (346, 388, 1781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4651) [M::mem_pestat] mean and std.dev: (779.84, 885.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6086) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (293, 1748, 2610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7244) [M::mem_pestat] mean and std.dev: (1628.30, 1433.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9561) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1200.878 CPU sec, 52.859 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1029437, 46, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 361, 1282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3166) [M::mem_pestat] mean and std.dev: (793.30, 713.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4108) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (185, 2047, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7430) [M::mem_pestat] mean and std.dev: (1658.80, 1683.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9845) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (447, 1344, 2342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6132) [M::mem_pestat] mean and std.dev: (1497.50, 930.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8027) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1243.545 CPU sec, 74.660 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1029320, 62, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 394, 1993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5325) [M::mem_pestat] mean and std.dev: (1081.70, 1094.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6991) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (333, 2584, 2609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7161) [M::mem_pestat] mean and std.dev: (1811.33, 1430.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9437) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (927, 1574, 2781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6489) [M::mem_pestat] mean and std.dev: (1743.08, 1132.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8343) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1206.478 CPU sec, 54.992 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1029421, 39, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (268, 360, 3199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9061) [M::mem_pestat] mean and std.dev: (1386.74, 1833.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11992) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (677, 2589, 2612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6482) [M::mem_pestat] mean and std.dev: (1705.03, 1182.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8417) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (430, 1565, 2917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7891) [M::mem_pestat] mean and std.dev: (1670.33, 1389.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10378) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1198.190 CPU sec, 65.311 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1028791, 39, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (321, 365, 2199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5955) [M::mem_pestat] mean and std.dev: (1079.96, 1354.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (479, 2419, 2598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6836) [M::mem_pestat] mean and std.dev: (1802.70, 1459.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8955) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1205.999 CPU sec, 53.205 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1029554, 40, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 370, 3523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9937) [M::mem_pestat] mean and std.dev: (1575.38, 2088.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13144) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1015, 2595, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5785) [M::mem_pestat] mean and std.dev: (1813.92, 1063.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7375) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1207.813 CPU sec, 65.819 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1029600, 41, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (339, 371, 880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1962) [M::mem_pestat] mean and std.dev: (572.67, 342.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (386, 2586, 2615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7073) [M::mem_pestat] mean and std.dev: (1727.03, 1146.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9302) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (980, 1977, 5443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14369) [M::mem_pestat] mean and std.dev: (2934.82, 2534.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18832) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1200.446 CPU sec, 52.836 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1028883, 59, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 335, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (178, 523) [M::mem_pestat] mean and std.dev: (332.32, 32.94) [M::mem_pestat] low and high boundaries for proper pairs: (109, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (263, 1932, 2625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7349) [M::mem_pestat] mean and std.dev: (1826.54, 1747.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9711) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (407, 1084, 3753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10445) [M::mem_pestat] mean and std.dev: (1984.23, 1972.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13791) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1254.216 CPU sec, 75.085 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1029595, 54, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 356, 1217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2993) [M::mem_pestat] mean and std.dev: (773.65, 773.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (532, 2012, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6745) [M::mem_pestat] mean and std.dev: (1593.73, 1153.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8816) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (397, 805, 1490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3676) [M::mem_pestat] mean and std.dev: (782.92, 489.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4769) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1219.885 CPU sec, 55.678 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1028955, 39, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 349, 1889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5043) [M::mem_pestat] mean and std.dev: (707.17, 819.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (418, 2582, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6985) [M::mem_pestat] mean and std.dev: (1797.63, 1477.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9174) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (424, 954, 2175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5677) [M::mem_pestat] mean and std.dev: (1020.50, 719.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7428) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1211.934 CPU sec, 65.703 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1030148, 40, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (295, 356, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1225) [M::mem_pestat] mean and std.dev: (373.29, 124.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (273, 2588, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7275) [M::mem_pestat] mean and std.dev: (1931.03, 1505.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9609) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (558, 1463, 2744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7116) [M::mem_pestat] mean and std.dev: (1376.86, 975.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9302) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1192.579 CPU sec, 52.478 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1029619, 41, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 345, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (160, 540) [M::mem_pestat] mean and std.dev: (315.35, 58.55) [M::mem_pestat] low and high boundaries for proper pairs: (81, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.46, 11.49) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (291, 1908, 2599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7215) [M::mem_pestat] mean and std.dev: (1579.05, 1114.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9523) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (418, 1594, 2322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6130) [M::mem_pestat] mean and std.dev: (1485.69, 1032.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8034) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1212.022 CPU sec, 67.063 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 1028024, 47, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (308, 364, 1046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2522) [M::mem_pestat] mean and std.dev: (694.88, 616.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3260) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.49) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (317, 2577, 2610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7196) [M::mem_pestat] mean and std.dev: (1622.72, 1262.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9489) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (811, 1084, 1488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2842) [M::mem_pestat] mean and std.dev: (1109.40, 410.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3519) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1201.735 CPU sec, 52.964 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1029336, 55, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (335, 360, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (103, 683) [M::mem_pestat] mean and std.dev: (351.00, 46.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1105, 1929, 2628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5674) [M::mem_pestat] mean and std.dev: (1870.54, 1242.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7197) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1210, 1487, 2756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5848) [M::mem_pestat] mean and std.dev: (1295.90, 832.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7394) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1255.397 CPU sec, 75.352 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1029400, 42, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 358, 1342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3416) [M::mem_pestat] mean and std.dev: (715.43, 643.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (306, 1707, 2612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7224) [M::mem_pestat] mean and std.dev: (1645.55, 1431.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9530) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (430, 1531, 2997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8131) [M::mem_pestat] mean and std.dev: (1698.29, 1706.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10698) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1207.893 CPU sec, 55.140 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1029022, 66, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (298, 356, 1615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4249) [M::mem_pestat] mean and std.dev: (743.42, 702.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (777, 2595, 3220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8106) [M::mem_pestat] mean and std.dev: (2120.18, 1504.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10549) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (534, 1104, 1812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4368) [M::mem_pestat] mean and std.dev: (1268.62, 1024.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5646) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1214.439 CPU sec, 66.945 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1029498, 52, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 321, 568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100) [M::mem_pestat] mean and std.dev: (345.94, 180.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (773, 1593, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6272) [M::mem_pestat] mean and std.dev: (1578.47, 1328.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8105) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1206.477 CPU sec, 53.137 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1029106, 51, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 354, 1055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2505) [M::mem_pestat] mean and std.dev: (618.10, 563.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3230) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (109, 1898, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7591) [M::mem_pestat] mean and std.dev: (1490.63, 1411.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10085) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (409, 822, 1619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4039) [M::mem_pestat] mean and std.dev: (1026.92, 768.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5249) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1215.915 CPU sec, 65.299 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1028932, 37, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 344, 981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2483) [M::mem_pestat] mean and std.dev: (478.91, 500.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3234) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (629, 2593, 3141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8165) [M::mem_pestat] mean and std.dev: (2116.44, 1751.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10677) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (976, 1700, 1845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3583) [M::mem_pestat] mean and std.dev: (1342.50, 685.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4452) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1192.408 CPU sec, 52.524 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1027999, 41, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (351, 369, 752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1554) [M::mem_pestat] mean and std.dev: (389.25, 181.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1955) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1874, 2586, 3368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6356) [M::mem_pestat] mean and std.dev: (1950.41, 1140.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7850) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (589, 1432, 2682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6868) [M::mem_pestat] mean and std.dev: (1352.67, 951.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8961) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1258.606 CPU sec, 75.101 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 1029247, 44, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 384, 1095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2731) [M::mem_pestat] mean and std.dev: (704.43, 718.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (422, 2583, 3759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10433) [M::mem_pestat] mean and std.dev: (2672.91, 2667.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13770) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1209.964 CPU sec, 55.228 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 1029495, 53, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 358, 1079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2589) [M::mem_pestat] mean and std.dev: (541.83, 451.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3344) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (599, 2015, 2957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7673) [M::mem_pestat] mean and std.dev: (2060.43, 1741.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10031) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (402, 1126, 2074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5418) [M::mem_pestat] mean and std.dev: (1336.22, 1242.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7090) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1213.122 CPU sec, 64.879 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1029908, 52, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (322, 406, 2286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6214) [M::mem_pestat] mean and std.dev: (1270.58, 1595.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8178) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1423, 2592, 2959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6031) [M::mem_pestat] mean and std.dev: (2024.78, 1323.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7567) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1204.307 CPU sec, 53.058 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1029502, 59, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (337, 363, 2064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5518) [M::mem_pestat] mean and std.dev: (961.26, 1220.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7245) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (337, 2326, 2947) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8167) [M::mem_pestat] mean and std.dev: (1836.47, 1648.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10777) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (447, 1074, 1794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4488) [M::mem_pestat] mean and std.dev: (1061.00, 614.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5835) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1211.483 CPU sec, 65.263 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1028822, 52, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 348, 1009) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2547) [M::mem_pestat] mean and std.dev: (562.71, 584.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3316) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (635, 2577, 2601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6533) [M::mem_pestat] mean and std.dev: (1874.78, 1319.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8499) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1202.560 CPU sec, 52.966 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1028439, 48, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (369, 462, 1726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4440) [M::mem_pestat] mean and std.dev: (861.11, 802.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1242, 2588, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5334) [M::mem_pestat] mean and std.dev: (2056.39, 1362.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7508) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (355, 944, 3110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8620) [M::mem_pestat] mean and std.dev: (1673.92, 1932.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11375) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1252.321 CPU sec, 74.862 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1029480, 46, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (320, 354, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1700) [M::mem_pestat] mean and std.dev: (441.00, 285.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2160) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (385, 2586, 2611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7063) [M::mem_pestat] mean and std.dev: (1907.68, 1514.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9289) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (403, 1618, 2587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6955) [M::mem_pestat] mean and std.dev: (1620.82, 1318.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9139) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1219.427 CPU sec, 55.692 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 1029617, 60, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 377, 880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1982) [M::mem_pestat] mean and std.dev: (487.12, 365.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (135, 1890, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7551) [M::mem_pestat] mean and std.dev: (1604.33, 1538.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10023) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (393, 684, 1396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3402) [M::mem_pestat] mean and std.dev: (697.70, 519.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4405) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1208.059 CPU sec, 59.345 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1028876, 42, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (342, 369, 887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1977) [M::mem_pestat] mean and std.dev: (560.52, 467.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (118, 2577, 2615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7609) [M::mem_pestat] mean and std.dev: (2022.76, 1787.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10106) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1014, 1332, 2541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5595) [M::mem_pestat] mean and std.dev: (1606.92, 1104.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7122) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1199.548 CPU sec, 52.792 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1028568, 46, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (313, 426, 1286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3232) [M::mem_pestat] mean and std.dev: (730.81, 621.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4205) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (259, 1477, 2598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7276) [M::mem_pestat] mean and std.dev: (1672.09, 1579.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9615) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (597, 1447, 5197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14397) [M::mem_pestat] mean and std.dev: (2424.65, 2421.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18997) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1212.610 CPU sec, 66.597 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 1029526, 44, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 372, 1118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2730) [M::mem_pestat] mean and std.dev: (514.11, 420.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (320, 2073, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7181) [M::mem_pestat] mean and std.dev: (1681.29, 1249.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9468) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1205.302 CPU sec, 53.239 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1029455, 39, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (345, 629, 1606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4128) [M::mem_pestat] mean and std.dev: (793.19, 655.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5389) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (385, 1896, 2610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7060) [M::mem_pestat] mean and std.dev: (1557.32, 1169.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9285) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1629, 2754, 3791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8115) [M::mem_pestat] mean and std.dev: (2643.40, 1429.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10277) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1190.755 CPU sec, 52.455 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 1029952, 55, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (315, 347, 1434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3672) [M::mem_pestat] mean and std.dev: (680.68, 650.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (336, 2586, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7149) [M::mem_pestat] mean and std.dev: (1786.80, 1343.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9420) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1248.689 CPU sec, 77.364 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1029196, 44, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 387, 1004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2384) [M::mem_pestat] mean and std.dev: (503.37, 338.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3074) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (170, 2585, 2723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7829) [M::mem_pestat] mean and std.dev: (1728.45, 1552.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10382) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1210.426 CPU sec, 54.717 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1028091, 53, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (345, 414, 2448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6654) [M::mem_pestat] mean and std.dev: (1302.82, 1538.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.45) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1058, 2517, 2614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5726) [M::mem_pestat] mean and std.dev: (1826.40, 1314.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7282) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (642, 1022, 1664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3708) [M::mem_pestat] mean and std.dev: (1104.23, 664.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4730) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1208.455 CPU sec, 61.877 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1030029, 40, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (294, 356, 968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2316) [M::mem_pestat] mean and std.dev: (543.22, 498.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2990) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (646, 2594, 2794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7090) [M::mem_pestat] mean and std.dev: (2059.79, 1325.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9238) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (410, 648, 2383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6329) [M::mem_pestat] mean and std.dev: (1273.50, 1697.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8302) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1208.957 CPU sec, 53.195 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1029711, 52, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (268, 345, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (329.77, 98.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.50, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (618, 2586, 2609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6591) [M::mem_pestat] mean and std.dev: (1929.67, 1257.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8582) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (961, 1556, 2013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4117) [M::mem_pestat] mean and std.dev: (1183.22, 627.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5169) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1207.897 CPU sec, 66.312 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1028688, 40, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (337, 369, 745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1561) [M::mem_pestat] mean and std.dev: (495.14, 262.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (414, 2578, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6981) [M::mem_pestat] mean and std.dev: (1745.21, 1183.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9170) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1005, 1320, 1909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3717) [M::mem_pestat] mean and std.dev: (1323.50, 822.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4621) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1207.655 CPU sec, 53.268 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1028956, 51, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 357, 614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1210) [M::mem_pestat] mean and std.dev: (389.36, 277.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (309, 2093, 2594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7164) [M::mem_pestat] mean and std.dev: (1675.43, 1364.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9449) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (411, 464, 1344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3210) [M::mem_pestat] mean and std.dev: (842.93, 732.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4143) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1256.853 CPU sec, 75.246 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1028960, 46, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (346, 557, 1348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3352) [M::mem_pestat] mean and std.dev: (771.41, 754.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (98, 1647, 2597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7595) [M::mem_pestat] mean and std.dev: (1515.58, 1642.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10094) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (855, 1226, 1448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2634) [M::mem_pestat] mean and std.dev: (963.00, 353.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3227) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1211.127 CPU sec, 55.218 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1029254, 61, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (350, 757, 1184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2852) [M::mem_pestat] mean and std.dev: (823.00, 601.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (260, 2506, 2612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7316) [M::mem_pestat] mean and std.dev: (1654.96, 1338.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9668) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (376, 1224, 1971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5161) [M::mem_pestat] mean and std.dev: (1359.23, 1108.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6756) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1213.148 CPU sec, 68.019 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 1028259, 51, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 345, 1315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3269) [M::mem_pestat] mean and std.dev: (983.17, 1061.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5228) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (985, 2590, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5851) [M::mem_pestat] mean and std.dev: (1863.95, 1188.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7473) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1211.929 CPU sec, 53.493 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1029770, 47, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (333, 469, 939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2151) [M::mem_pestat] mean and std.dev: (552.85, 313.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (262, 1902, 2595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7261) [M::mem_pestat] mean and std.dev: (1519.80, 1318.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9594) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1206.065 CPU sec, 65.775 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1029273, 63, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (320, 344, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (104, 644) [M::mem_pestat] mean and std.dev: (333.74, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (420, 2589, 2614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7002) [M::mem_pestat] mean and std.dev: (1921.95, 1375.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9196) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (520, 1262, 1494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3442) [M::mem_pestat] mean and std.dev: (1001.36, 581.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4416) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1204.677 CPU sec, 53.036 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1029625, 55, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (309, 404, 1191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2955) [M::mem_pestat] mean and std.dev: (635.18, 497.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (824, 2586, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6152) [M::mem_pestat] mean and std.dev: (1755.14, 1144.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7928) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (403, 499, 3128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8578) [M::mem_pestat] mean and std.dev: (1465.29, 1591.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11303) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1264.510 CPU sec, 75.793 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1028431, 47, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (344, 372, 790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1682) [M::mem_pestat] mean and std.dev: (417.52, 155.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2128) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (752, 2586, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6317) [M::mem_pestat] mean and std.dev: (1885.46, 1364.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8172) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (356, 735, 1480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3728) [M::mem_pestat] mean and std.dev: (781.78, 678.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4852) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1227.291 CPU sec, 56.207 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 1028749, 65, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 338, 1767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4703) [M::mem_pestat] mean and std.dev: (968.19, 1113.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6171) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (276, 1872, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7269) [M::mem_pestat] mean and std.dev: (1560.78, 1350.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9600) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1213.244 CPU sec, 66.910 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1029801, 52, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 360, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066) [M::mem_pestat] mean and std.dev: (339.33, 155.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (433, 2580, 2597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6925) [M::mem_pestat] mean and std.dev: (1798.37, 1191.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9089) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1216.765 CPU sec, 53.809 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1029366, 36, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 372, 3946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11210) [M::mem_pestat] mean and std.dev: (2197.31, 2838.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.38) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (276, 2589, 2822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7914) [M::mem_pestat] mean and std.dev: (1924.21, 1509.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10460) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1239.123 CPU sec, 71.545 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1030314, 54, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (308, 567, 1872) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5000) [M::mem_pestat] mean and std.dev: (881.13, 880.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1305, 2590, 2610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5220) [M::mem_pestat] mean and std.dev: (2009.12, 1228.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6923) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (893, 1543, 2913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6953) [M::mem_pestat] mean and std.dev: (2037.30, 1811.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9283) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1218.160 CPU sec, 53.716 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1029295, 42, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 720, 3387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9493) [M::mem_pestat] mean and std.dev: (1965.48, 2343.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.41, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (568, 2301, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6682) [M::mem_pestat] mean and std.dev: (1928.08, 1525.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8720) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (642, 1336, 2835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7221) [M::mem_pestat] mean and std.dev: (1927.88, 1787.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9414) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1269.879 CPU sec, 75.551 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1029261, 55, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 363, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (40, 750) [M::mem_pestat] mean and std.dev: (344.29, 86.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.51, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (504, 2590, 2623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6861) [M::mem_pestat] mean and std.dev: (1956.02, 1429.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8980) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (392, 641, 1270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3026) [M::mem_pestat] mean and std.dev: (609.89, 354.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3904) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1218.982 CPU sec, 55.435 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1029431, 46, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 376, 902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2048) [M::mem_pestat] mean and std.dev: (545.38, 423.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (545, 2584, 2955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7775) [M::mem_pestat] mean and std.dev: (2133.09, 1820.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10185) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (769, 1094, 4500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11962) [M::mem_pestat] mean and std.dev: (2267.00, 2632.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15693) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1234.629 CPU sec, 68.467 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1029205, 53, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 789, 1633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4271) [M::mem_pestat] mean and std.dev: (983.67, 882.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (250, 1737, 2596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7288) [M::mem_pestat] mean and std.dev: (1630.20, 1581.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9634) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1215.550 CPU sec, 53.657 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1028550, 55, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (323, 362, 1647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4295) [M::mem_pestat] mean and std.dev: (753.35, 714.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (495, 2121, 2604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6822) [M::mem_pestat] mean and std.dev: (1760.80, 1332.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8931) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (390, 715, 1163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2709) [M::mem_pestat] mean and std.dev: (721.42, 506.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3482) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1232.202 CPU sec, 66.097 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1029713, 39, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (306, 353, 779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1725) [M::mem_pestat] mean and std.dev: (408.36, 225.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2198) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (619, 2581, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6583) [M::mem_pestat] mean and std.dev: (1987.03, 1423.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8571) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (812, 1287, 3181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7919) [M::mem_pestat] mean and std.dev: (2014.36, 1758.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10288) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1210.031 CPU sec, 53.269 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1029466, 41, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 373, 1017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2397) [M::mem_pestat] mean and std.dev: (599.62, 532.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3087) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (270, 1897, 2598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7254) [M::mem_pestat] mean and std.dev: (1575.35, 1098.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9582) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (580, 913, 1695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3925) [M::mem_pestat] mean and std.dev: (1039.75, 663.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5040) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1265.222 CPU sec, 75.704 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1029465, 41, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (309, 358, 1039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2499) [M::mem_pestat] mean and std.dev: (690.71, 692.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (413, 2585, 2611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7007) [M::mem_pestat] mean and std.dev: (1978.08, 1554.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9205) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1368, 2219, 6646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17202) [M::mem_pestat] mean and std.dev: (3183.00, 2921.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22480) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1228.337 CPU sec, 56.286 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1029150, 46, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (341, 382, 1494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3800) [M::mem_pestat] mean and std.dev: (649.75, 536.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (272, 1765, 2604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7268) [M::mem_pestat] mean and std.dev: (1692.65, 1388.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9600) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (370, 496, 1088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2524) [M::mem_pestat] mean and std.dev: (703.60, 507.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3242) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1230.194 CPU sec, 65.484 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1029612, 48, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (309, 393, 1346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3420) [M::mem_pestat] mean and std.dev: (847.17, 822.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.50, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (279, 2494, 2599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7239) [M::mem_pestat] mean and std.dev: (1816.22, 1388.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9559) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1207.532 CPU sec, 53.195 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 1030037, 45, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 378, 720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1500) [M::mem_pestat] mean and std.dev: (413.25, 163.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1890) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (990, 2051, 2943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6849) [M::mem_pestat] mean and std.dev: (2000.59, 1602.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8802) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1250, 1634, 3660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8480) [M::mem_pestat] mean and std.dev: (2455.50, 1887.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10890) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1214.672 CPU sec, 66.765 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1028729, 39, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (323, 343, 631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1247) [M::mem_pestat] mean and std.dev: (408.11, 231.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (276, 1881, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7257) [M::mem_pestat] mean and std.dev: (1632.44, 1371.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9584) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (437, 819, 2152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5582) [M::mem_pestat] mean and std.dev: (1136.85, 936.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7297) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1205.999 CPU sec, 53.150 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 1029046, 65, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (309, 385, 1194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2964) [M::mem_pestat] mean and std.dev: (713.77, 672.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3849) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1115, 2594, 3633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8669) [M::mem_pestat] mean and std.dev: (3141.55, 2758.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14177) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (584, 1288, 1490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3302) [M::mem_pestat] mean and std.dev: (1050.00, 519.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4208) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1272.419 CPU sec, 76.202 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1028967, 50, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (242, 351, 1076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2744) [M::mem_pestat] mean and std.dev: (435.91, 354.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1252, 2591, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5314) [M::mem_pestat] mean and std.dev: (1926.13, 1059.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6668) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1222.727 CPU sec, 56.019 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1028730, 37, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (332, 380, 1028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2420) [M::mem_pestat] mean and std.dev: (432.62, 299.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3116) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (217, 1027, 2598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7360) [M::mem_pestat] mean and std.dev: (1275.64, 1072.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9741) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (392, 832, 1861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4799) [M::mem_pestat] mean and std.dev: (1036.67, 913.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6268) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1214.337 CPU sec, 65.523 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1029391, 38, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 370, 1569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4049) [M::mem_pestat] mean and std.dev: (814.61, 827.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5289) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (260, 2591, 2610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7310) [M::mem_pestat] mean and std.dev: (1931.64, 1557.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9660) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1203.041 CPU sec, 53.058 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1029414, 48, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 307, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (67, 592) [M::mem_pestat] mean and std.dev: (297.94, 97.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (802, 2577, 2838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6910) [M::mem_pestat] mean and std.dev: (1848.70, 1144.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8946) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (910, 1128, 1842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3706) [M::mem_pestat] mean and std.dev: (1070.92, 591.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4638) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1211.799 CPU sec, 65.171 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 1029959, 42, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (295, 362, 827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1891) [M::mem_pestat] mean and std.dev: (430.24, 323.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (264, 2581, 2599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7269) [M::mem_pestat] mean and std.dev: (1815.55, 1546.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9604) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (752, 1374, 1834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3998) [M::mem_pestat] mean and std.dev: (1271.42, 661.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5080) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1213.072 CPU sec, 53.478 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1029166, 52, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (335, 354, 1209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2957) [M::mem_pestat] mean and std.dev: (629.50, 632.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1863, 2592, 2640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (309, 4194) [M::mem_pestat] mean and std.dev: (2375.90, 727.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5287) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (439, 1161, 1853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4681) [M::mem_pestat] mean and std.dev: (956.20, 672.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1262.473 CPU sec, 75.668 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1029929, 52, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 344, 2011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5373) [M::mem_pestat] mean and std.dev: (909.35, 932.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7054) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1567, 2593, 2609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4693) [M::mem_pestat] mean and std.dev: (2027.19, 1229.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6947) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (405, 731, 937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2001) [M::mem_pestat] mean and std.dev: (623.75, 285.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2533) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1226.869 CPU sec, 55.921 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1029398, 38, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (333, 689, 2308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6258) [M::mem_pestat] mean and std.dev: (1284.26, 1507.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8233) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (134, 1557, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7532) [M::mem_pestat] mean and std.dev: (1435.46, 1151.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9998) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (387, 898, 1461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3609) [M::mem_pestat] mean and std.dev: (877.75, 490.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4683) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1215.040 CPU sec, 64.969 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 1028738, 43, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 429, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000) [M::mem_pestat] mean and std.dev: (398.00, 87.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1220) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (382, 2077, 2601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7039) [M::mem_pestat] mean and std.dev: (1499.00, 1054.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9258) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (371, 631, 3140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8678) [M::mem_pestat] mean and std.dev: (1488.33, 1513.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11447) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1213.553 CPU sec, 53.542 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1028611, 56, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (303, 345, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (165, 510) [M::mem_pestat] mean and std.dev: (317.08, 47.07) [M::mem_pestat] low and high boundaries for proper pairs: (96, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.42) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (217, 1497, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7381) [M::mem_pestat] mean and std.dev: (1490.42, 1225.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9769) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1498, 2878, 5318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12958) [M::mem_pestat] mean and std.dev: (2793.75, 2026.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16778) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1219.442 CPU sec, 65.116 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 1028739, 54, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (315, 347, 591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1143) [M::mem_pestat] mean and std.dev: (367.86, 162.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (525, 2585, 2880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7590) [M::mem_pestat] mean and std.dev: (1861.47, 1350.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9945) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (773, 883, 1776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3782) [M::mem_pestat] mean and std.dev: (1144.00, 575.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4785) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1218.765 CPU sec, 53.700 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1030250, 57, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (326, 365, 1172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2864) [M::mem_pestat] mean and std.dev: (750.28, 743.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3725) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (293, 1973, 2609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7241) [M::mem_pestat] mean and std.dev: (1704.06, 1376.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9557) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (392, 873, 2342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6242) [M::mem_pestat] mean and std.dev: (1279.69, 1014.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8192) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1197.251 CPU sec, 52.892 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1029153, 49, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (295, 354, 1106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2728) [M::mem_pestat] mean and std.dev: (501.00, 406.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (414, 2006, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6990) [M::mem_pestat] mean and std.dev: (1842.34, 1335.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9182) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (534, 889, 1330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2922) [M::mem_pestat] mean and std.dev: (854.58, 416.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3718) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1258.067 CPU sec, 75.250 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1029447, 47, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 351, 1086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2590) [M::mem_pestat] mean and std.dev: (588.89, 513.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3342) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.50, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (953, 2588, 2599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5891) [M::mem_pestat] mean and std.dev: (1841.42, 1293.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7537) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1186, 1798, 2729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5815) [M::mem_pestat] mean and std.dev: (1923.00, 991.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7358) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1217.232 CPU sec, 55.468 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1028914, 56, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (320, 351, 1088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2624) [M::mem_pestat] mean and std.dev: (459.69, 294.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (928, 2584, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5962) [M::mem_pestat] mean and std.dev: (1834.06, 1174.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7640) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (326, 1405, 3236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9056) [M::mem_pestat] mean and std.dev: (2336.71, 2444.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12113) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1212.601 CPU sec, 64.050 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1028723, 45, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 374, 1913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5129) [M::mem_pestat] mean and std.dev: (856.53, 1045.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (273, 2255, 2609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7281) [M::mem_pestat] mean and std.dev: (1720.23, 1320.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9617) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1213.741 CPU sec, 53.556 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1029363, 51, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (307, 378, 878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2020) [M::mem_pestat] mean and std.dev: (535.83, 401.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1245, 2590, 2632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5406) [M::mem_pestat] mean and std.dev: (2060.54, 1389.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7617) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1210.779 CPU sec, 59.788 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1029109, 51, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (352, 367, 1003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2305) [M::mem_pestat] mean and std.dev: (577.76, 469.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2956) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (304, 1974, 2610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7222) [M::mem_pestat] mean and std.dev: (1632.86, 1277.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9528) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1025, 1685, 2870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6560) [M::mem_pestat] mean and std.dev: (1577.00, 1006.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8405) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1206.466 CPU sec, 53.211 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1029769, 51, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 368, 1596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4184) [M::mem_pestat] mean and std.dev: (855.50, 848.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.45) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (619, 1977, 2644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6694) [M::mem_pestat] mean and std.dev: (1858.17, 1534.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8719) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (372, 1159, 2982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8202) [M::mem_pestat] mean and std.dev: (1844.36, 2206.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10812) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1256.075 CPU sec, 75.436 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1029028, 39, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (310, 370, 830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1870) [M::mem_pestat] mean and std.dev: (504.04, 355.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1153, 2200, 2601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5497) [M::mem_pestat] mean and std.dev: (1635.81, 1002.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6945) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (588, 964, 1502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3330) [M::mem_pestat] mean and std.dev: (1063.07, 682.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4244) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1220.301 CPU sec, 55.787 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1029522, 67, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 436, 1231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3115) [M::mem_pestat] mean and std.dev: (789.00, 829.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4108) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (108, 2348, 2601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7587) [M::mem_pestat] mean and std.dev: (1532.33, 1445.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10080) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1211.010 CPU sec, 65.994 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 1029384, 50, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 331, 761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1773) [M::mem_pestat] mean and std.dev: (397.75, 294.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2279) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (524, 2575, 3743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10181) [M::mem_pestat] mean and std.dev: (2972.26, 2827.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14282) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (480, 1287, 2567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6741) [M::mem_pestat] mean and std.dev: (1610.69, 1488.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8828) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1204.577 CPU sec, 53.081 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 1029756, 47, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (322, 368, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1555) [M::mem_pestat] mean and std.dev: (452.65, 273.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1966) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (420, 1917, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6969) [M::mem_pestat] mean and std.dev: (1578.53, 1206.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9152) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (938, 1265, 2839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6641) [M::mem_pestat] mean and std.dev: (2197.45, 1880.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9720) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1218.136 CPU sec, 67.384 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 1029907, 52, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 338, 723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1623) [M::mem_pestat] mean and std.dev: (370.52, 228.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2073) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (578, 2099, 2604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6656) [M::mem_pestat] mean and std.dev: (1847.72, 1450.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8682) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (700, 1313, 4754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12862) [M::mem_pestat] mean and std.dev: (2221.85, 1866.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16916) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1209.227 CPU sec, 53.392 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 1029430, 47, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 394, 1544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3972) [M::mem_pestat] mean and std.dev: (818.30, 791.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5186) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (270, 2572, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7275) [M::mem_pestat] mean and std.dev: (1820.82, 1392.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9610) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1270.551 CPU sec, 75.652 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 1028864, 46, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 363, 636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1248) [M::mem_pestat] mean and std.dev: (391.70, 228.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (622, 2595, 2912) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7492) [M::mem_pestat] mean and std.dev: (2255.11, 1730.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9782) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1216.296 CPU sec, 55.484 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1029693, 35, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (336, 362, 1224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3000) [M::mem_pestat] mean and std.dev: (646.76, 628.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3888) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (611, 1912, 2594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6560) [M::mem_pestat] mean and std.dev: (1646.75, 1096.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8543) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1215.161 CPU sec, 67.183 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1029801, 51, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (284, 357, 604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1244) [M::mem_pestat] mean and std.dev: (338.72, 137.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1291, 2595, 2618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5272) [M::mem_pestat] mean and std.dev: (1996.68, 1185.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6737) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1090, 1441, 4400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11020) [M::mem_pestat] mean and std.dev: (2776.91, 2447.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14330) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1204.646 CPU sec, 53.253 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1028725, 50, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 346, 677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1397) [M::mem_pestat] mean and std.dev: (451.65, 291.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (250, 2256, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7309) [M::mem_pestat] mean and std.dev: (1687.91, 1558.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9662) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1067, 1785, 4838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12380) [M::mem_pestat] mean and std.dev: (2828.23, 2535.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16151) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1211.328 CPU sec, 65.852 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 1029504, 48, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (294, 337, 774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1734) [M::mem_pestat] mean and std.dev: (450.27, 405.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2214) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (448, 2588, 2985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8059) [M::mem_pestat] mean and std.dev: (2080.43, 1578.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10596) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (926, 1596, 2093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4427) [M::mem_pestat] mean and std.dev: (1327.40, 665.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5594) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1216.028 CPU sec, 53.644 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 1029121, 49, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 328, 1184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3050) [M::mem_pestat] mean and std.dev: (699.07, 855.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4121) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (276, 2437, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7266) [M::mem_pestat] mean and std.dev: (1954.12, 1803.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9596) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (521, 1308, 1911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4691) [M::mem_pestat] mean and std.dev: (1386.33, 1036.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6081) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1261.965 CPU sec, 75.271 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1029847, 41, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (355, 506, 2276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6118) [M::mem_pestat] mean and std.dev: (1281.26, 1511.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8039) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1625, 2588, 3362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6836) [M::mem_pestat] mean and std.dev: (2257.05, 1501.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8573) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (384, 952, 3497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9723) [M::mem_pestat] mean and std.dev: (2174.10, 2213.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12836) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1219.884 CPU sec, 55.609 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1028180, 37, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (335, 362, 1244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3062) [M::mem_pestat] mean and std.dev: (643.76, 533.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3971) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (148, 2010, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7522) [M::mem_pestat] mean and std.dev: (1645.56, 1477.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9980) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1233.850 CPU sec, 68.984 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1028982, 45, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 340, 2018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5450) [M::mem_pestat] mean and std.dev: (801.11, 971.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7166) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (94, 2588, 2608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7636) [M::mem_pestat] mean and std.dev: (1876.19, 1540.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10150) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (315, 414, 665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1365) [M::mem_pestat] mean and std.dev: (421.25, 175.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1715) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1214.253 CPU sec, 53.644 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1029563, 54, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 351, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (381.59, 171.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (769, 2592, 2619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6319) [M::mem_pestat] mean and std.dev: (1735.96, 1164.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8169) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1213.731 CPU sec, 67.730 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1030034, 48, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 341, 2019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5403) [M::mem_pestat] mean and std.dev: (971.64, 1026.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7095) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (635, 2587, 2829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7217) [M::mem_pestat] mean and std.dev: (1857.67, 1330.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9411) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (667, 999, 2512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6202) [M::mem_pestat] mean and std.dev: (1250.31, 972.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8047) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1204.419 CPU sec, 53.320 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1028411, 48, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 349, 762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1654) [M::mem_pestat] mean and std.dev: (479.12, 355.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2100) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (291, 1909, 2598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7212) [M::mem_pestat] mean and std.dev: (1559.50, 1156.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9519) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (916, 1432, 2461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5551) [M::mem_pestat] mean and std.dev: (1845.33, 1742.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8815) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1267.381 CPU sec, 75.268 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1029695, 48, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 347, 1117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2911) [M::mem_pestat] mean and std.dev: (547.33, 490.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (368, 2576, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7079) [M::mem_pestat] mean and std.dev: (1885.84, 1447.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9316) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1227.950 CPU sec, 56.453 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1029336, 65, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (278, 339, 908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2168) [M::mem_pestat] mean and std.dev: (484.71, 471.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (321, 1904, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7167) [M::mem_pestat] mean and std.dev: (1727.52, 1422.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9449) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1228.091 CPU sec, 68.074 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1028803, 55, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 387, 1050) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2474) [M::mem_pestat] mean and std.dev: (557.50, 398.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3186) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (632, 2586, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6551) [M::mem_pestat] mean and std.dev: (1686.06, 1222.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8524) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (624, 1274, 2089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5019) [M::mem_pestat] mean and std.dev: (1292.64, 928.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6484) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1204.276 CPU sec, 53.174 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1029056, 40, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (304, 365, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (62, 667) [M::mem_pestat] mean and std.dev: (330.56, 70.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (272, 2254, 2971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8369) [M::mem_pestat] mean and std.dev: (1981.50, 1764.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11068) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1222.779 CPU sec, 68.332 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1028478, 41, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (351, 367, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1638) [M::mem_pestat] mean and std.dev: (497.69, 301.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2067) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (276, 2587, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7257) [M::mem_pestat] mean and std.dev: (1806.74, 1271.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9584) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (471, 1071, 1754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4320) [M::mem_pestat] mean and std.dev: (1147.53, 834.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5603) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1224.589 CPU sec, 54.143 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1029270, 55, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (282, 360, 1170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2946) [M::mem_pestat] mean and std.dev: (687.77, 656.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1069, 2598, 2913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6601) [M::mem_pestat] mean and std.dev: (1852.62, 1345.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8445) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (388, 1058, 2735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7429) [M::mem_pestat] mean and std.dev: (1573.00, 1376.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9776) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1258.668 CPU sec, 75.830 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1029408, 47, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (296, 379, 1329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3395) [M::mem_pestat] mean and std.dev: (800.00, 700.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (273, 1871, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7263) [M::mem_pestat] mean and std.dev: (1629.00, 1333.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9593) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (430, 1403, 1922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4906) [M::mem_pestat] mean and std.dev: (1368.77, 885.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6398) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1223.638 CPU sec, 55.600 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1030035, 56, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (319, 356, 1197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2953) [M::mem_pestat] mean and std.dev: (626.21, 684.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3831) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (621, 2592, 2870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7368) [M::mem_pestat] mean and std.dev: (1988.44, 1463.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9617) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (677, 1170, 1756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3914) [M::mem_pestat] mean and std.dev: (1141.77, 767.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4993) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1210.436 CPU sec, 60.398 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 1028449, 61, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (352, 527, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2191) [M::mem_pestat] mean and std.dev: (674.73, 419.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.45) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (477, 2584, 2604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6858) [M::mem_pestat] mean and std.dev: (1891.11, 1471.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8985) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (387, 834, 1294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3108) [M::mem_pestat] mean and std.dev: (859.50, 487.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4015) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1215.657 CPU sec, 53.652 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 1029329, 39, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 359, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (201, 491) [M::mem_pestat] mean and std.dev: (341.20, 63.84) [M::mem_pestat] low and high boundaries for proper pairs: (86, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.51, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (130, 1414, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7561) [M::mem_pestat] mean and std.dev: (1427.63, 1601.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10038) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1043, 2080, 5373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14033) [M::mem_pestat] mean and std.dev: (2679.92, 2388.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18363) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1211.777 CPU sec, 65.119 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1028819, 52, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (331, 363, 1312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3274) [M::mem_pestat] mean and std.dev: (844.95, 900.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.46, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1133, 2582, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5552) [M::mem_pestat] mean and std.dev: (1756.31, 970.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7025) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1012, 1429, 1582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2722) [M::mem_pestat] mean and std.dev: (1146.70, 491.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3292) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1215.134 CPU sec, 53.506 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1029305, 49, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 319, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (52, 582) [M::mem_pestat] mean and std.dev: (290.07, 81.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 688) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (284, 2579, 2599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7229) [M::mem_pestat] mean and std.dev: (1824.75, 1355.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9544) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (659, 988, 2035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4787) [M::mem_pestat] mean and std.dev: (1130.82, 833.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6163) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1197.967 CPU sec, 52.894 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 1029274, 59, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 373, 1592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4096) [M::mem_pestat] mean and std.dev: (867.86, 954.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (286, 2589, 2613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7267) [M::mem_pestat] mean and std.dev: (1845.98, 1600.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9594) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (807, 2008, 7014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 19428) [M::mem_pestat] mean and std.dev: (3347.20, 3244.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 25635) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2666668 sequences (240000120 bp)... [M::mem_process_seqs] Processed 2666668 reads in 1230.858 CPU sec, 78.287 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1029015, 51, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (341, 356, 1132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2714) [M::mem_pestat] mean and std.dev: (806.32, 745.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (946, 2053, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5923) [M::mem_pestat] mean and std.dev: (1814.02, 1256.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7582) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (694, 1212, 1797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4003) [M::mem_pestat] mean and std.dev: (1214.80, 667.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5106) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1214.587 CPU sec, 53.988 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1028546, 39, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (288, 363, 1378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3558) [M::mem_pestat] mean and std.dev: (724.12, 737.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (910, 2586, 2630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6070) [M::mem_pestat] mean and std.dev: (1910.09, 1412.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7790) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (427, 1716, 4433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12445) [M::mem_pestat] mean and std.dev: (2622.50, 2656.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16451) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1211.739 CPU sec, 66.370 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1028801, 54, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (357, 403, 1726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4464) [M::mem_pestat] mean and std.dev: (539.77, 389.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5833) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1484, 2590, 2608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4856) [M::mem_pestat] mean and std.dev: (2041.10, 1063.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6297) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1197.373 CPU sec, 52.669 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1028632, 54, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 376, 1706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4508) [M::mem_pestat] mean and std.dev: (857.20, 819.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5909) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (270, 1894, 2590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7230) [M::mem_pestat] mean and std.dev: (1566.40, 1335.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9550) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (389, 748, 4061) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11405) [M::mem_pestat] mean and std.dev: (2222.69, 2604.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15077) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1212.271 CPU sec, 67.430 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 1028515, 55, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (291, 352, 1266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3216) [M::mem_pestat] mean and std.dev: (760.33, 797.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (898, 2580, 2611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6037) [M::mem_pestat] mean and std.dev: (1783.60, 1224.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7750) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (606, 875, 2400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5988) [M::mem_pestat] mean and std.dev: (1036.42, 751.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7782) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1204.218 CPU sec, 53.047 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1028908, 52, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (355, 804, 2564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6982) [M::mem_pestat] mean and std.dev: (1204.26, 1373.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9191) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1320, 2588, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5160) [M::mem_pestat] mean and std.dev: (1918.69, 1138.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6474) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (589, 1775, 1986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4780) [M::mem_pestat] mean and std.dev: (1475.50, 891.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6177) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1257.259 CPU sec, 75.760 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1028449, 57, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 403, 3015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8391) [M::mem_pestat] mean and std.dev: (1423.40, 1800.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11079) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (276, 2301, 2609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7275) [M::mem_pestat] mean and std.dev: (1663.09, 1320.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9608) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (548, 1005, 2262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5690) [M::mem_pestat] mean and std.dev: (1247.58, 1151.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7404) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1226.104 CPU sec, 56.554 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (34, 1028553, 50, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (321, 361, 886) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2016) [M::mem_pestat] mean and std.dev: (453.00, 359.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (264, 2587, 2613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7311) [M::mem_pestat] mean and std.dev: (1679.09, 1348.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9660) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1213.146 CPU sec, 66.183 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1029309, 45, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 377, 1097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2713) [M::mem_pestat] mean and std.dev: (674.82, 603.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (252, 2563, 2601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7299) [M::mem_pestat] mean and std.dev: (1607.32, 1326.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9648) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (420, 862, 5336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15168) [M::mem_pestat] mean and std.dev: (2010.38, 2148.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20084) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1218.184 CPU sec, 53.805 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 1029196, 54, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 367, 1188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3002) [M::mem_pestat] mean and std.dev: (516.73, 436.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3909) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (237, 2581, 2601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7329) [M::mem_pestat] mean and std.dev: (1777.80, 1416.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9693) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (592, 1685, 3072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8032) [M::mem_pestat] mean and std.dev: (1896.69, 1648.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10512) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1216.047 CPU sec, 66.716 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1028356, 57, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (322, 344, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (84, 679) [M::mem_pestat] mean and std.dev: (317.71, 114.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (474, 2592, 2941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7875) [M::mem_pestat] mean and std.dev: (1978.56, 1313.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10342) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1206.111 CPU sec, 53.237 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1028981, 57, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (297, 335, 964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2298) [M::mem_pestat] mean and std.dev: (492.22, 477.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.41, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (281, 2487, 2602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7244) [M::mem_pestat] mean and std.dev: (1585.53, 1293.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9565) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (400, 1807, 5325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15175) [M::mem_pestat] mean and std.dev: (2370.92, 2412.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20100) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1261.064 CPU sec, 75.693 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1030114, 35, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (301, 357, 1207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3019) [M::mem_pestat] mean and std.dev: (641.67, 514.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3925) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.46, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (2096, 2596, 2949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (390, 4655) [M::mem_pestat] mean and std.dev: (2407.15, 798.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5600) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (446, 1314, 2302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6014) [M::mem_pestat] mean and std.dev: (1157.20, 700.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7870) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1221.304 CPU sec, 55.584 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1029598, 47, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (295, 496, 1367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3511) [M::mem_pestat] mean and std.dev: (849.41, 695.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (270, 2575, 2626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7338) [M::mem_pestat] mean and std.dev: (1865.31, 1580.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9694) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (586, 1164, 3804) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10240) [M::mem_pestat] mean and std.dev: (1530.62, 1329.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13458) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1205.863 CPU sec, 64.080 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 1028602, 45, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 346, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 904) [M::mem_pestat] mean and std.dev: (282.65, 171.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.45) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (414, 2091, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6981) [M::mem_pestat] mean and std.dev: (1667.68, 1306.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9170) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1206.994 CPU sec, 53.178 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1028895, 51, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (344, 839, 1881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4955) [M::mem_pestat] mean and std.dev: (764.65, 681.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1187, 2586, 2613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5465) [M::mem_pestat] mean and std.dev: (1884.40, 1245.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6891) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1217.778 CPU sec, 66.492 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1030045, 66, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (300, 340, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (76, 636) [M::mem_pestat] mean and std.dev: (331.67, 81.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (214, 1893, 2595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7357) [M::mem_pestat] mean and std.dev: (1517.13, 1262.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9738) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1210.419 CPU sec, 53.471 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1028696, 50, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (282, 362, 1290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3306) [M::mem_pestat] mean and std.dev: (661.55, 713.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4314) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (282, 2591, 2905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8151) [M::mem_pestat] mean and std.dev: (1975.54, 1679.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10774) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (533, 1021, 1802) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4340) [M::mem_pestat] mean and std.dev: (984.30, 579.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5609) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1254.692 CPU sec, 74.896 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1029115, 41, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 361, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (64, 689) [M::mem_pestat] mean and std.dev: (349.33, 52.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (393, 2581, 2604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7026) [M::mem_pestat] mean and std.dev: (2045.70, 1520.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9237) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (477, 858, 1565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3741) [M::mem_pestat] mean and std.dev: (1102.79, 816.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4829) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1215.546 CPU sec, 55.454 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 1028934, 43, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 353, 1344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3504) [M::mem_pestat] mean and std.dev: (695.81, 663.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (282, 1872, 2601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7239) [M::mem_pestat] mean and std.dev: (1497.08, 1155.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9558) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (401, 1005, 1612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4034) [M::mem_pestat] mean and std.dev: (907.94, 724.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5245) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1215.280 CPU sec, 66.722 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1029406, 44, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (326, 347, 792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1724) [M::mem_pestat] mean and std.dev: (544.50, 386.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2190) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (591, 2591, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6636) [M::mem_pestat] mean and std.dev: (1860.02, 1222.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8651) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1204.995 CPU sec, 53.170 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1029304, 45, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (357, 481, 2045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5421) [M::mem_pestat] mean and std.dev: (1230.00, 1415.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7109) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1705, 2591, 2953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5449) [M::mem_pestat] mean and std.dev: (1935.78, 1188.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6697) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1213.037 CPU sec, 65.014 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 1029443, 44, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 380, 996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2410) [M::mem_pestat] mean and std.dev: (595.41, 500.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3117) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (266, 1856, 2595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7253) [M::mem_pestat] mean and std.dev: (1491.98, 1141.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9582) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1179, 1824, 3320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7602) [M::mem_pestat] mean and std.dev: (1842.00, 1275.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9743) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1195.228 CPU sec, 52.611 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1029087, 53, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 369, 1095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2617) [M::mem_pestat] mean and std.dev: (504.45, 379.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3378) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (263, 1860, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7283) [M::mem_pestat] mean and std.dev: (1598.32, 1323.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9623) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (303, 500, 2705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7509) [M::mem_pestat] mean and std.dev: (1676.08, 2359.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11115) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1264.528 CPU sec, 75.330 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1028839, 49, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 331, 885) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2089) [M::mem_pestat] mean and std.dev: (459.79, 349.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (947, 2590, 3007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7127) [M::mem_pestat] mean and std.dev: (1877.14, 1369.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9187) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (928, 2216, 3047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7285) [M::mem_pestat] mean and std.dev: (1815.89, 986.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9404) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1217.512 CPU sec, 55.417 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1029554, 53, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 378, 1345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3359) [M::mem_pestat] mean and std.dev: (929.05, 947.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (560, 2584, 2950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7730) [M::mem_pestat] mean and std.dev: (2047.96, 1692.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10120) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1206.405 CPU sec, 64.682 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1028479, 40, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 345, 1160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2902) [M::mem_pestat] mean and std.dev: (492.16, 401.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3773) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (495, 1479, 2597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6801) [M::mem_pestat] mean and std.dev: (1516.69, 1135.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8903) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (533, 1663, 2931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7727) [M::mem_pestat] mean and std.dev: (1660.80, 1500.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10125) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1197.838 CPU sec, 52.754 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1028816, 49, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (249, 316, 772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1818) [M::mem_pestat] mean and std.dev: (367.30, 279.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2341) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (279, 2062, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7257) [M::mem_pestat] mean and std.dev: (1554.34, 1267.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9583) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (501, 1744, 3160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8478) [M::mem_pestat] mean and std.dev: (2346.93, 2299.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11543) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1208.120 CPU sec, 65.270 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1028739, 44, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (337, 373, 1361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3409) [M::mem_pestat] mean and std.dev: (601.77, 521.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (269, 2579, 2671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7475) [M::mem_pestat] mean and std.dev: (1596.10, 1273.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9877) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1208.237 CPU sec, 53.240 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 1029226, 46, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (310, 347, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (144, 559) [M::mem_pestat] mean and std.dev: (346.10, 43.64) [M::mem_pestat] low and high boundaries for proper pairs: (61, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (425, 2591, 2610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6980) [M::mem_pestat] mean and std.dev: (2020.05, 1490.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9165) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1251.458 CPU sec, 75.160 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1028915, 50, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (318, 357, 858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1938) [M::mem_pestat] mean and std.dev: (390.62, 242.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (119, 1934, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7571) [M::mem_pestat] mean and std.dev: (1389.93, 1162.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10055) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (719, 1221, 1420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2822) [M::mem_pestat] mean and std.dev: (1115.58, 519.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3523) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1213.040 CPU sec, 55.302 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1028817, 43, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (318, 336, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975) [M::mem_pestat] mean and std.dev: (364.50, 168.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (225, 2299, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7350) [M::mem_pestat] mean and std.dev: (1431.02, 1244.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9725) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1202.031 CPU sec, 63.951 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 1029552, 47, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 396, 1212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3074) [M::mem_pestat] mean and std.dev: (752.41, 768.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4005) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1340, 2526, 2618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5174) [M::mem_pestat] mean and std.dev: (1851.76, 1239.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6808) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1194.785 CPU sec, 52.591 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 1029336, 48, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (339, 378, 923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2091) [M::mem_pestat] mean and std.dev: (581.28, 368.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (372, 2580, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7074) [M::mem_pestat] mean and std.dev: (1616.95, 1168.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9308) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1215.545 CPU sec, 67.105 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1029516, 43, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (298, 351, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 763) [M::mem_pestat] mean and std.dev: (344.57, 117.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (813, 2587, 2612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6210) [M::mem_pestat] mean and std.dev: (1937.95, 1326.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8009) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (389, 815, 1388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3386) [M::mem_pestat] mean and std.dev: (1048.50, 919.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4727) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1204.222 CPU sec, 53.214 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1029912, 61, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 361, 2027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5631) [M::mem_pestat] mean and std.dev: (858.67, 1079.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7433) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (267, 2595, 2954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8328) [M::mem_pestat] mean and std.dev: (1940.78, 1505.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11015) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1247.685 CPU sec, 74.827 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1028708, 37, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 357, 1847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4937) [M::mem_pestat] mean and std.dev: (794.21, 973.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (267, 2242, 2609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7293) [M::mem_pestat] mean and std.dev: (1883.76, 1633.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9635) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (546, 1291, 6084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17160) [M::mem_pestat] mean and std.dev: (2660.67, 2830.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22698) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1215.182 CPU sec, 55.678 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1029694, 45, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (290, 340, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1934) [M::mem_pestat] mean and std.dev: (449.04, 399.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1253, 2594, 3799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8891) [M::mem_pestat] mean and std.dev: (2685.84, 2207.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11516) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1088, 1265, 3978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9758) [M::mem_pestat] mean and std.dev: (2153.38, 1944.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12648) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1204.415 CPU sec, 64.363 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1029643, 50, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (320, 371, 862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1946) [M::mem_pestat] mean and std.dev: (577.00, 424.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.48) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (875, 2596, 3267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8051) [M::mem_pestat] mean and std.dev: (2153.95, 1665.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10443) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1196.003 CPU sec, 52.646 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1028861, 50, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 348, 807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1789) [M::mem_pestat] mean and std.dev: (529.70, 460.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2372) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (389, 1956, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7022) [M::mem_pestat] mean and std.dev: (1604.51, 1178.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9233) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (464, 1130, 1828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4556) [M::mem_pestat] mean and std.dev: (836.75, 531.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5920) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1204.509 CPU sec, 64.764 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1029455, 47, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (309, 333, 1244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3114) [M::mem_pestat] mean and std.dev: (703.58, 751.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4049) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1395, 2598, 3441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7533) [M::mem_pestat] mean and std.dev: (2469.52, 1762.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9579) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (462, 605, 1023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2145) [M::mem_pestat] mean and std.dev: (565.78, 283.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2706) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1198.680 CPU sec, 52.754 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1029372, 60, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 1028, 2075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5591) [M::mem_pestat] mean and std.dev: (1213.25, 1102.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7349) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (606, 2586, 2611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6621) [M::mem_pestat] mean and std.dev: (1773.60, 1149.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8626) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (609, 1085, 1866) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4380) [M::mem_pestat] mean and std.dev: (1097.64, 711.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5637) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1194.128 CPU sec, 52.666 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 1029188, 45, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 349, 633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1251) [M::mem_pestat] mean and std.dev: (399.52, 187.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1194, 2597, 3096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6900) [M::mem_pestat] mean and std.dev: (1951.64, 1304.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8802) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (777, 1479, 2234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5148) [M::mem_pestat] mean and std.dev: (1568.25, 1152.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6605) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1252.000 CPU sec, 75.056 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 1028636, 47, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 363, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (301.79, 93.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (419, 2576, 2614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7004) [M::mem_pestat] mean and std.dev: (1966.96, 1403.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9199) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (970, 1131, 2138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4474) [M::mem_pestat] mean and std.dev: (1354.33, 673.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5642) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1214.012 CPU sec, 55.388 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 1030317, 46, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (315, 360, 811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1803) [M::mem_pestat] mean and std.dev: (453.00, 336.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2299) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (276, 1499, 2592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7224) [M::mem_pestat] mean and std.dev: (1253.15, 1132.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9540) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (827, 1519, 2456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5714) [M::mem_pestat] mean and std.dev: (1552.83, 1232.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7343) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1203.309 CPU sec, 65.492 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 1029386, 63, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (344, 368, 650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1262) [M::mem_pestat] mean and std.dev: (406.33, 153.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.38) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (461, 2590, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6896) [M::mem_pestat] mean and std.dev: (1705.81, 1225.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9041) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (439, 1375, 2705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7237) [M::mem_pestat] mean and std.dev: (1741.79, 1593.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9503) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1200.326 CPU sec, 52.867 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1029383, 43, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 343, 1437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3727) [M::mem_pestat] mean and std.dev: (551.05, 510.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4872) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (128, 1897, 2610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7574) [M::mem_pestat] mean and std.dev: (1459.36, 1188.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10056) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1213.229 CPU sec, 66.339 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 1029477, 48, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 375, 978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2280) [M::mem_pestat] mean and std.dev: (579.75, 538.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2931) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1170, 2590, 2902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6366) [M::mem_pestat] mean and std.dev: (2144.53, 1449.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8098) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (441, 1278, 1745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4353) [M::mem_pestat] mean and std.dev: (1140.31, 751.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5657) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1198.293 CPU sec, 52.803 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1028491, 52, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (337, 441, 1498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3820) [M::mem_pestat] mean and std.dev: (864.61, 759.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1398, 2589, 2794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5586) [M::mem_pestat] mean and std.dev: (1904.19, 1028.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6982) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (428, 754, 1677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4175) [M::mem_pestat] mean and std.dev: (985.92, 928.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5424) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1259.877 CPU sec, 75.558 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 1029351, 48, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (333, 348, 1614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4176) [M::mem_pestat] mean and std.dev: (576.37, 498.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5457) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1026, 2585, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5769) [M::mem_pestat] mean and std.dev: (1914.20, 1274.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7350) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (767, 1859, 3417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8717) [M::mem_pestat] mean and std.dev: (2142.18, 1874.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11367) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1207.676 CPU sec, 54.833 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1029135, 50, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 336, 924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2226) [M::mem_pestat] mean and std.dev: (504.30, 484.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (374, 2580, 2868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7856) [M::mem_pestat] mean and std.dev: (1893.27, 1662.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10350) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (446, 1316, 2049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5255) [M::mem_pestat] mean and std.dev: (1208.00, 818.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6858) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1210.353 CPU sec, 68.097 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 1029673, 49, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 357, 2192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5952) [M::mem_pestat] mean and std.dev: (894.42, 1004.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7832) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (285, 1884, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7230) [M::mem_pestat] mean and std.dev: (1604.91, 1162.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9545) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (623, 1246, 4233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11453) [M::mem_pestat] mean and std.dev: (2033.42, 1986.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15063) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1208.323 CPU sec, 53.346 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1028756, 55, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 356, 761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1757) [M::mem_pestat] mean and std.dev: (548.95, 519.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.45) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (278, 2594, 2857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8015) [M::mem_pestat] mean and std.dev: (1894.73, 1727.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10594) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (781, 1839, 2831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6931) [M::mem_pestat] mean and std.dev: (2077.80, 1837.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9429) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1224.636 CPU sec, 68.347 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 1029219, 33, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 381, 2095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5635) [M::mem_pestat] mean and std.dev: (1018.67, 1220.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.46, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (478, 2588, 2614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6886) [M::mem_pestat] mean and std.dev: (1991.48, 1433.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9022) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1128, 2321, 4713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11883) [M::mem_pestat] mean and std.dev: (2454.58, 1740.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15468) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1204.609 CPU sec, 53.136 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1028509, 64, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 366, 1349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3419) [M::mem_pestat] mean and std.dev: (702.20, 656.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1090, 2589, 2928) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6604) [M::mem_pestat] mean and std.dev: (2031.60, 1300.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8442) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1255.842 CPU sec, 75.379 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1030062, 38, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 344, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (78, 613) [M::mem_pestat] mean and std.dev: (315.00, 57.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (154, 2584, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7510) [M::mem_pestat] mean and std.dev: (1560.70, 1572.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9962) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (740, 1449, 3918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10274) [M::mem_pestat] mean and std.dev: (2237.75, 2022.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13452) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1199.126 CPU sec, 54.691 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1029037, 59, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 435, 1944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5172) [M::mem_pestat] mean and std.dev: (850.30, 809.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (964, 2590, 2905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6787) [M::mem_pestat] mean and std.dev: (1967.59, 1289.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8728) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (423, 1795, 2547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6795) [M::mem_pestat] mean and std.dev: (1925.20, 1610.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8919) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1216.573 CPU sec, 65.291 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 1030037, 47, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (322, 361, 2488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6820) [M::mem_pestat] mean and std.dev: (1503.40, 2125.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.50, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (611, 2582, 2602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6584) [M::mem_pestat] mean and std.dev: (1731.89, 1172.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8575) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (783, 1229, 2781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6777) [M::mem_pestat] mean and std.dev: (1451.70, 1055.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8775) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1203.248 CPU sec, 53.126 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 1029239, 55, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (311, 341, 909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2105) [M::mem_pestat] mean and std.dev: (526.32, 454.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (272, 1911, 2602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7262) [M::mem_pestat] mean and std.dev: (1698.44, 1531.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9592) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (598, 978, 2722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6970) [M::mem_pestat] mean and std.dev: (1466.30, 1031.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9094) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1211.180 CPU sec, 65.388 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1029145, 34, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (332, 502, 1685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4391) [M::mem_pestat] mean and std.dev: (810.00, 701.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.45) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1139, 2582, 2601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5525) [M::mem_pestat] mean and std.dev: (1947.56, 1193.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6987) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1209.706 CPU sec, 53.322 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1029464, 41, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 352, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (72, 682) [M::mem_pestat] mean and std.dev: (335.00, 46.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (447, 2588, 2873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7725) [M::mem_pestat] mean and std.dev: (2188.31, 1789.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10151) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (773, 1452, 3347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8495) [M::mem_pestat] mean and std.dev: (1780.44, 1241.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11069) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1246.661 CPU sec, 75.051 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1029370, 61, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 382, 719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1533) [M::mem_pestat] mean and std.dev: (457.53, 377.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1966) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (374, 2503, 2609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7079) [M::mem_pestat] mean and std.dev: (1825.05, 1516.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9314) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (366, 773, 1763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4557) [M::mem_pestat] mean and std.dev: (954.50, 747.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5954) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1203.657 CPU sec, 54.970 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1029215, 45, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 386, 611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1165) [M::mem_pestat] mean and std.dev: (404.11, 147.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (322, 2191, 2597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7147) [M::mem_pestat] mean and std.dev: (1666.22, 1202.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9422) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (834, 1154, 2252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5088) [M::mem_pestat] mean and std.dev: (1531.64, 1238.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6506) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1222.248 CPU sec, 66.180 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1029460, 61, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 359, 1064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2532) [M::mem_pestat] mean and std.dev: (458.75, 298.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3266) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1125, 2566, 2611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5583) [M::mem_pestat] mean and std.dev: (1867.78, 1203.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7069) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (309, 713, 1277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3213) [M::mem_pestat] mean and std.dev: (725.00, 505.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4181) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1197.492 CPU sec, 52.853 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1029066, 35, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 345, 745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1567) [M::mem_pestat] mean and std.dev: (492.69, 333.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1978) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (932, 2585, 3119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7493) [M::mem_pestat] mean and std.dev: (2352.88, 1911.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9998) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (825, 1188, 1785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3705) [M::mem_pestat] mean and std.dev: (1397.55, 790.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4665) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1221.800 CPU sec, 68.134 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 1029106, 50, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (326, 402, 2100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5648) [M::mem_pestat] mean and std.dev: (877.45, 904.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.48, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1214, 2592, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5390) [M::mem_pestat] mean and std.dev: (1934.71, 1133.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6782) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (430, 702, 1654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4102) [M::mem_pestat] mean and std.dev: (1045.83, 691.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5326) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1202.281 CPU sec, 53.034 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1029539, 56, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 375, 853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1909) [M::mem_pestat] mean and std.dev: (502.13, 276.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.47, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (362, 2010, 2602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7082) [M::mem_pestat] mean and std.dev: (1857.98, 1453.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9322) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (413, 710, 2590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6944) [M::mem_pestat] mean and std.dev: (1444.09, 1394.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9121) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1251.834 CPU sec, 74.862 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1028744, 50, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 359, 901) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2023) [M::mem_pestat] mean and std.dev: (438.39, 245.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.42, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (244, 2565, 2610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7342) [M::mem_pestat] mean and std.dev: (1833.37, 1411.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9708) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (704, 1120, 1822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4058) [M::mem_pestat] mean and std.dev: (1014.42, 576.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5176) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1219.605 CPU sec, 55.654 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1029003, 53, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (318, 353, 652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1320) [M::mem_pestat] mean and std.dev: (372.32, 126.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1115, 2588, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5579) [M::mem_pestat] mean and std.dev: (1884.58, 1060.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7067) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1208.988 CPU sec, 65.795 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1029429, 42, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 546, 1840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4852) [M::mem_pestat] mean and std.dev: (843.33, 721.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (629, 2596, 2960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7622) [M::mem_pestat] mean and std.dev: (2152.67, 1642.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9953) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (847, 1932, 2649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6253) [M::mem_pestat] mean and std.dev: (1914.60, 1397.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8055) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1200.788 CPU sec, 52.900 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 1028206, 56, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 375, 973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2251) [M::mem_pestat] mean and std.dev: (640.33, 573.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1316, 2591, 2615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5213) [M::mem_pestat] mean and std.dev: (2088.25, 1160.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6731) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1210.507 CPU sec, 66.328 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 1029232, 51, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (339, 373, 840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1842) [M::mem_pestat] mean and std.dev: (435.62, 260.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2343) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.49, 11.39) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1906, 2594, 2615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (488, 4033) [M::mem_pestat] mean and std.dev: (2334.89, 567.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4742) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (445, 535, 1497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3601) [M::mem_pestat] mean and std.dev: (802.43, 621.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4653) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1199.507 CPU sec, 52.874 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::process] read 2666668 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 1029345, 41, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 353, 2319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6431) [M::mem_pestat] mean and std.dev: (896.73, 1017.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (703, 2591, 2951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7447) [M::mem_pestat] mean and std.dev: (2394.28, 1893.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9969) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (560, 1186, 1974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4802) [M::mem_pestat] mean and std.dev: (1375.73, 1019.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6216) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2666668 reads in 1260.362 CPU sec, 75.856 real sec [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1028813, 51, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (301, 347, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 958) [M::mem_pestat] mean and std.dev: (349.33, 178.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1177) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (266, 1827, 2598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7262) [M::mem_pestat] mean and std.dev: (1609.52, 1400.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9594) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1219.581 CPU sec, 55.729 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 1029573, 50, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 359, 972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2332) [M::mem_pestat] mean and std.dev: (655.64, 550.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3012) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.44, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (747, 2587, 2608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6330) [M::mem_pestat] mean and std.dev: (1849.35, 1187.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8191) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1213.891 CPU sec, 66.351 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 1029456, 47, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 357, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971) [M::mem_pestat] mean and std.dev: (374.10, 96.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.43, 11.47) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (679, 1971, 2598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6436) [M::mem_pestat] mean and std.dev: (1830.74, 1221.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8355) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1197.289 CPU sec, 52.734 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 1029117, 48, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (321, 350, 1583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4107) [M::mem_pestat] mean and std.dev: (825.83, 802.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5369) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (466, 474, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (434, 514) [M::mem_pestat] mean and std.dev: (474.45, 11.46) [M::mem_pestat] low and high boundaries for proper pairs: (418, 530) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (443, 2001, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6923) [M::mem_pestat] mean and std.dev: (1591.62, 1188.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9083) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1219.467 CPU sec, 66.835 real sec [M::process] read 2666668 sequences (240000120 bp)... [M::process] 0 single-end sequences; 2666668 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 1027947, 56, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 387, 1392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3648) [M::mem_pestat] mean and std.dev: (698.94, 618.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4776) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.50, 11.41) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (1013, 2115, 2598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5768) [M::mem_pestat] mean and std.dev: (1832.82, 1348.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7353) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2666668 reads in 1205.957 CPU sec, 53.113 real sec [M::process] read 2640828 sequences (237674520 bp)... [M::process] 0 single-end sequences; 2640828 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 1018859, 41, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (341, 365, 1735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4523) [M::mem_pestat] mean and std.dev: (909.43, 975.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (467, 475, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (437, 512) [M::mem_pestat] mean and std.dev: (474.50, 11.40) [M::mem_pestat] low and high boundaries for proper pairs: (422, 527) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (302, 2587, 2865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7991) [M::mem_pestat] mean and std.dev: (1797.08, 1460.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10554) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (407, 1498, 2301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6089) [M::mem_pestat] mean and std.dev: (1350.00, 1265.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7983) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2640828 reads in 1195.069 CPU sec, 51.265 real sec [main] Version: 0.7.17-r1188 [main] CMD: /home/csyk2proj/tools/bwa.kit/bwa mem -p -t24 -R@RG\tID:KPGP-00220\tPL:ILLUMINA\tSM:KPGP-00220 -C /home/csyk2proj/data/bwa.kit/hs38DH.fa - [main] Real time: 49332.091 sec; CPU: 543076.074 sec