[M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (151, 954250, 88, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 143, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (133.46, 67.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.75, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 59, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (81.31, 96.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 268, 1190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3212) [M::mem_pestat] mean and std.dev: (664.45, 725.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4223) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1221.720 CPU sec, 74.047 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (148, 953655, 70, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 135, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (129.85, 76.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 53, 87) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 197) [M::mem_pestat] mean and std.dev: (47.13, 31.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 252) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 365, 1056) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2906) [M::mem_pestat] mean and std.dev: (535.96, 428.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3831) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1188.135 CPU sec, 54.155 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (150, 954616, 82, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 117, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (120.13, 71.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.86, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 54, 937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2745) [M::mem_pestat] mean and std.dev: (199.47, 433.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3649) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 258, 849) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2205) [M::mem_pestat] mean and std.dev: (428.25, 409.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2883) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1200.240 CPU sec, 71.903 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (165, 954084, 90, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 132, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (129.67, 73.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 66, 2172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6440) [M::mem_pestat] mean and std.dev: (991.44, 1638.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8574) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 602, 981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2537) [M::mem_pestat] mean and std.dev: (685.31, 577.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3315) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1182.213 CPU sec, 52.157 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (160, 954440, 94, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 110, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (124.01, 75.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 49, 79) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 173) [M::mem_pestat] mean and std.dev: (43.88, 23.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 220) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 246, 984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2560) [M::mem_pestat] mean and std.dev: (517.17, 519.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3348) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1197.890 CPU sec, 73.440 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 954232, 88, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 133, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (127.64, 72.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 62, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1305) [M::mem_pestat] mean and std.dev: (99.46, 182.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1727) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 567, 1135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2987) [M::mem_pestat] mean and std.dev: (661.20, 554.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3913) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1176.836 CPU sec, 51.842 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (169, 954017, 83, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 150, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (139.15, 66.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.93) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 58, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1442) [M::mem_pestat] mean and std.dev: (104.73, 217.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1911) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 254, 874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2234) [M::mem_pestat] mean and std.dev: (397.06, 386.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2914) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1212.933 CPU sec, 72.646 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (160, 954916, 94, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 125, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (119.92, 64.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 57, 1114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3272) [M::mem_pestat] mean and std.dev: (319.38, 707.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4351) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 208, 724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1896) [M::mem_pestat] mean and std.dev: (458.90, 511.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2503) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1176.934 CPU sec, 53.567 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (177, 954386, 96, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 120, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (122.04, 71.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 63, 1776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5256) [M::mem_pestat] mean and std.dev: (795.33, 1420.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6996) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 257, 961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2467) [M::mem_pestat] mean and std.dev: (562.41, 623.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3220) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1189.130 CPU sec, 71.715 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 954804, 69, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 124, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (114.97, 68.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 53, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (71.75, 95.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 208, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 808) [M::mem_pestat] mean and std.dev: (249.64, 204.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1067) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1172.926 CPU sec, 51.943 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (155, 955293, 96, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 132, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (124.99, 63.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 63, 1435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4227) [M::mem_pestat] mean and std.dev: (382.17, 859.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5623) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 211, 579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1439) [M::mem_pestat] mean and std.dev: (310.51, 335.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1190.563 CPU sec, 69.769 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (154, 953746, 95, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 119, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (123.90, 73.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 46, 67) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 139) [M::mem_pestat] mean and std.dev: (41.59, 19.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 175) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 224, 808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2134) [M::mem_pestat] mean and std.dev: (463.55, 515.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2797) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1175.487 CPU sec, 51.769 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (150, 954772, 95, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 119, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (117.70, 62.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 58, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (54.45, 40.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 620, 1123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2929) [M::mem_pestat] mean and std.dev: (684.72, 630.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3832) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1195.345 CPU sec, 53.630 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (196, 954562, 73, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 123, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (118.47, 70.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.91) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 48, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041) [M::mem_pestat] mean and std.dev: (62.16, 98.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1377) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (126, 266, 824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2220) [M::mem_pestat] mean and std.dev: (464.90, 493.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2918) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1225.449 CPU sec, 73.618 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (158, 954921, 87, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 130, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (128.12, 70.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 58, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (67.40, 78.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 257, 1012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2664) [M::mem_pestat] mean and std.dev: (567.10, 606.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3490) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1180.771 CPU sec, 53.403 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (138, 953909, 93, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 126, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (121.51, 64.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 48, 97) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 237) [M::mem_pestat] mean and std.dev: (52.03, 39.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 307) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 245, 1017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2651) [M::mem_pestat] mean and std.dev: (556.10, 563.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3468) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1211.595 CPU sec, 73.153 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 953895, 91, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 134, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (132.58, 72.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 58, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (82.22, 143.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1217) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 281, 1940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5466) [M::mem_pestat] mean and std.dev: (964.59, 1186.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7229) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1186.268 CPU sec, 52.464 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (171, 954848, 86, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 125, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (125.55, 69.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 55, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988) [M::mem_pestat] mean and std.dev: (69.80, 98.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1307) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 262, 1313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3567) [M::mem_pestat] mean and std.dev: (751.09, 776.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4694) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1214.185 CPU sec, 72.120 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (145, 954307, 77, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 128, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (123.78, 67.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.74, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 50, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 216) [M::mem_pestat] mean and std.dev: (43.40, 24.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 277) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 239, 475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1081) [M::mem_pestat] mean and std.dev: (272.81, 179.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1384) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1184.150 CPU sec, 52.154 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (165, 953853, 107, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 113, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (115.84, 63.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 52, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1541) [M::mem_pestat] mean and std.dev: (150.75, 328.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2044) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 242, 990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2614) [M::mem_pestat] mean and std.dev: (497.88, 546.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3426) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1254.765 CPU sec, 74.856 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (175, 954384, 79, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 122, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (127.57, 74.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 52, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 194) [M::mem_pestat] mean and std.dev: (42.58, 23.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 249) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 259, 1379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3773) [M::mem_pestat] mean and std.dev: (740.67, 837.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4970) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1204.703 CPU sec, 54.665 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (143, 954640, 87, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 130, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (124.23, 65.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 53, 916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2682) [M::mem_pestat] mean and std.dev: (197.93, 527.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3565) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 256, 1134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2986) [M::mem_pestat] mean and std.dev: (709.58, 763.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3912) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1202.270 CPU sec, 72.381 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (164, 954362, 61, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 134, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (127.56, 69.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 52, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 182) [M::mem_pestat] mean and std.dev: (43.33, 27.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 235) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 688, 1405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3787) [M::mem_pestat] mean and std.dev: (725.93, 654.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4978) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1206.983 CPU sec, 53.367 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (164, 954693, 80, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 132, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (130.69, 67.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.88, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 50, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (55.80, 62.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 212, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1027) [M::mem_pestat] mean and std.dev: (273.68, 227.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1321) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1232.961 CPU sec, 71.821 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (161, 954484, 94, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 136, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (126.83, 66.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 51, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (49.95, 41.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 374) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 278, 989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2661) [M::mem_pestat] mean and std.dev: (562.97, 540.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3497) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1200.989 CPU sec, 52.875 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (171, 955072, 80, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 134, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (132.48, 70.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 52, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 190) [M::mem_pestat] mean and std.dev: (46.27, 29.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 243) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (126, 230, 787) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2109) [M::mem_pestat] mean and std.dev: (346.94, 316.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2770) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1196.266 CPU sec, 58.042 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (164, 953577, 74, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 131, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (127.33, 71.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 68, 1586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4688) [M::mem_pestat] mean and std.dev: (858.84, 1516.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6925) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 213, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (177.07, 67.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1231.912 CPU sec, 75.826 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 954294, 69, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 127, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (123.04, 69.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 54, 1205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3539) [M::mem_pestat] mean and std.dev: (190.95, 473.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4706) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 250, 1033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2699) [M::mem_pestat] mean and std.dev: (623.76, 689.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3532) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1198.719 CPU sec, 53.274 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (153, 954758, 82, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 137, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (130.41, 65.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 56, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1319) [M::mem_pestat] mean and std.dev: (75.41, 133.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1747) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 506, 1491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4085) [M::mem_pestat] mean and std.dev: (816.71, 828.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5382) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1231.377 CPU sec, 74.907 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (177, 953939, 101, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 134, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (125.88, 69.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 56, 876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2550) [M::mem_pestat] mean and std.dev: (161.84, 399.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3387) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 227, 833) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2207) [M::mem_pestat] mean and std.dev: (456.61, 512.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2894) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1207.699 CPU sec, 53.219 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (165, 954972, 75, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 127, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (121.70, 65.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.92) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 51, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 261) [M::mem_pestat] mean and std.dev: (46.59, 33.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 214, 1050) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2844) [M::mem_pestat] mean and std.dev: (534.05, 631.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3741) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1236.315 CPU sec, 75.570 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (158, 954457, 85, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 114, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (117.97, 66.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 57, 262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (68.27, 101.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 949) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 602, 1811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5065) [M::mem_pestat] mean and std.dev: (876.72, 979.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6692) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1208.800 CPU sec, 53.163 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (182, 954839, 79, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 123, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (118.37, 69.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 59, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (54.71, 43.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (90, 196, 1035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2925) [M::mem_pestat] mean and std.dev: (595.74, 752.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3870) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1236.463 CPU sec, 73.771 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (163, 954481, 83, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 134, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (130.22, 69.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 48, 78) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 174) [M::mem_pestat] mean and std.dev: (44.43, 25.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 222) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 255, 935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2469) [M::mem_pestat] mean and std.dev: (506.39, 490.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3236) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1206.898 CPU sec, 55.112 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (162, 954861, 77, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 123, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (119.03, 67.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 65, 2725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8103) [M::mem_pestat] mean and std.dev: (1025.94, 1849.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10792) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 241, 845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2195) [M::mem_pestat] mean and std.dev: (421.71, 411.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2870) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1235.158 CPU sec, 71.993 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (175, 954044, 78, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 124, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (122.91, 69.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 51, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (59.05, 64.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 218, 1176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3192) [M::mem_pestat] mean and std.dev: (634.96, 718.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4200) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1211.281 CPU sec, 53.511 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (166, 954622, 82, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 120, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (125.67, 70.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 63, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1639) [M::mem_pestat] mean and std.dev: (121.38, 250.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2174) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (119, 203, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (190.86, 112.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1226.816 CPU sec, 74.023 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (130, 953748, 67, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 115, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (116.86, 65.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 47, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (56.57, 70.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 278, 746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1814) [M::mem_pestat] mean and std.dev: (436.41, 325.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2348) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1207.235 CPU sec, 53.189 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (162, 953470, 89, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 143, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (133.99, 82.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 59, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (68.26, 62.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 249, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1264) [M::mem_pestat] mean and std.dev: (297.84, 301.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1641) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1216.130 CPU sec, 62.809 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (123, 954474, 78, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 119, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (124.88, 68.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 56, 865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2531) [M::mem_pestat] mean and std.dev: (125.90, 316.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3364) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 508, 1424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3854) [M::mem_pestat] mean and std.dev: (630.55, 600.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5069) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1231.140 CPU sec, 75.670 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (161, 954814, 81, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 135, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (133.81, 66.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 48, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 856) [M::mem_pestat] mean and std.dev: (65.73, 117.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 291, 963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2525) [M::mem_pestat] mean and std.dev: (550.66, 540.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3306) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1205.846 CPU sec, 53.066 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (151, 954147, 80, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 145, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (131.36, 74.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 52, 81) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 175) [M::mem_pestat] mean and std.dev: (44.46, 21.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 222) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 283, 1182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3186) [M::mem_pestat] mean and std.dev: (693.78, 747.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4188) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1223.239 CPU sec, 74.296 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (175, 954185, 71, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 136, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (139.16, 80.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 61, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (72.70, 96.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 304, 1022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2648) [M::mem_pestat] mean and std.dev: (593.24, 587.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3461) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1196.752 CPU sec, 52.660 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (165, 953680, 90, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 124, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (124.56, 69.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.75, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 59, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (61.43, 44.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 284, 914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2346) [M::mem_pestat] mean and std.dev: (493.00, 469.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3062) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1224.069 CPU sec, 73.209 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (168, 954031, 79, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 134, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (131.17, 72.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 49, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 156) [M::mem_pestat] mean and std.dev: (44.74, 25.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 198) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 243, 728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1900) [M::mem_pestat] mean and std.dev: (342.74, 353.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2486) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1215.141 CPU sec, 53.490 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (138, 954229, 89, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 122, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (120.15, 62.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 56, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1610) [M::mem_pestat] mean and std.dev: (95.03, 170.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2135) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 307, 795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1993) [M::mem_pestat] mean and std.dev: (516.45, 465.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2592) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.753 CPU sec, 73.512 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (154, 954778, 89, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 107, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (113.42, 70.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 59, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 601) [M::mem_pestat] mean and std.dev: (64.97, 59.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 249, 844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2164) [M::mem_pestat] mean and std.dev: (498.79, 528.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2824) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1199.960 CPU sec, 54.437 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 955018, 78, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 122, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (126.76, 70.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 53, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (60.05, 53.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 284, 1529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4181) [M::mem_pestat] mean and std.dev: (791.56, 857.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5507) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1241.882 CPU sec, 75.112 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (174, 953992, 81, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 137, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (128.84, 68.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 54, 702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2048) [M::mem_pestat] mean and std.dev: (149.09, 352.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2721) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 244, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1276) [M::mem_pestat] mean and std.dev: (254.18, 181.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1645) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1209.115 CPU sec, 53.405 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (169, 954430, 72, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 130, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (124.23, 69.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 55, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (59.00, 71.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 308, 923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2419) [M::mem_pestat] mean and std.dev: (543.88, 450.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3167) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1223.990 CPU sec, 72.462 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (153, 953612, 87, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 119, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (119.68, 69.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 49, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 153) [M::mem_pestat] mean and std.dev: (45.60, 22.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 193) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 227, 982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2664) [M::mem_pestat] mean and std.dev: (367.65, 370.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3505) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1204.839 CPU sec, 53.057 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (154, 954161, 87, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 148, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (136.82, 75.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 52, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2262) [M::mem_pestat] mean and std.dev: (166.61, 390.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3003) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 331, 1223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3295) [M::mem_pestat] mean and std.dev: (669.04, 686.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4331) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1219.392 CPU sec, 65.935 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (135, 954920, 90, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 129, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (129.59, 71.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 31.93) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 50, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 172) [M::mem_pestat] mean and std.dev: (46.46, 22.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 218) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 287, 1000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2604) [M::mem_pestat] mean and std.dev: (665.86, 605.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3406) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1216.966 CPU sec, 71.768 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (150, 954633, 71, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 143, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (138.86, 79.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 51, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (46.36, 39.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 261, 721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1861) [M::mem_pestat] mean and std.dev: (417.91, 406.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2431) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1203.060 CPU sec, 53.013 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (169, 954995, 102, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 131, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (124.05, 70.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 57, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (73.23, 121.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 248, 735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1931) [M::mem_pestat] mean and std.dev: (348.10, 328.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2529) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1231.033 CPU sec, 73.877 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (151, 954293, 78, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 128, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (122.96, 62.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 54, 119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (59.05, 50.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 253, 1151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3159) [M::mem_pestat] mean and std.dev: (530.36, 604.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4163) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1204.862 CPU sec, 53.005 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (129, 954822, 72, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 120, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (121.50, 65.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 74, 2403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7125) [M::mem_pestat] mean and std.dev: (1161.93, 1802.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9486) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 219, 677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1769) [M::mem_pestat] mean and std.dev: (333.93, 326.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2315) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1226.809 CPU sec, 74.538 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (175, 953942, 97, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 119, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (125.60, 74.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.73, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 59, 1289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3807) [M::mem_pestat] mean and std.dev: (352.28, 876.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5066) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 254, 1056) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2840) [M::mem_pestat] mean and std.dev: (614.72, 662.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3732) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1188.604 CPU sec, 52.299 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (136, 954203, 79, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 124, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (125.41, 66.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 31.93) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 57, 422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1196) [M::mem_pestat] mean and std.dev: (84.86, 153.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1583) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 653, 1224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3218) [M::mem_pestat] mean and std.dev: (696.61, 544.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4215) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1194.595 CPU sec, 52.413 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (174, 954093, 80, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 123, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (125.37, 71.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.75, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 57, 718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2084) [M::mem_pestat] mean and std.dev: (158.41, 350.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2767) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 812, 1310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3454) [M::mem_pestat] mean and std.dev: (835.33, 662.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4526) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1251.872 CPU sec, 74.364 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (160, 953492, 70, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 125, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (121.53, 64.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 56, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 185) [M::mem_pestat] mean and std.dev: (49.74, 29.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 236) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 215, 784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2038) [M::mem_pestat] mean and std.dev: (429.79, 495.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2665) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1213.248 CPU sec, 55.259 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (176, 954743, 71, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 139, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (132.50, 66.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 47, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 206) [M::mem_pestat] mean and std.dev: (41.48, 20.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 264) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 186, 917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2447) [M::mem_pestat] mean and std.dev: (480.41, 604.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3212) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1220.172 CPU sec, 73.262 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (175, 954093, 82, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 122, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (122.08, 67.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 52, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 235) [M::mem_pestat] mean and std.dev: (49.48, 30.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 302) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 274, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2533) [M::mem_pestat] mean and std.dev: (485.97, 471.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1201.309 CPU sec, 53.020 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (152, 955193, 82, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 132, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (128.77, 66.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.93) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 58, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (49.02, 37.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 249, 985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2657) [M::mem_pestat] mean and std.dev: (500.03, 523.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3493) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1229.617 CPU sec, 74.397 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (158, 954286, 93, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 120, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (125.30, 64.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 50, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 814) [M::mem_pestat] mean and std.dev: (62.82, 99.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 560, 1058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2780) [M::mem_pestat] mean and std.dev: (601.73, 487.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3641) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1211.656 CPU sec, 53.333 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (159, 954796, 94, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 140, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (127.79, 75.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 45, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (47.82, 48.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 240, 857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2285) [M::mem_pestat] mean and std.dev: (399.97, 453.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2999) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1236.447 CPU sec, 73.793 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (177, 954363, 94, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 128, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (127.10, 64.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 58, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 257) [M::mem_pestat] mean and std.dev: (54.32, 34.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 262, 759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1893) [M::mem_pestat] mean and std.dev: (438.55, 371.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2460) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1207.822 CPU sec, 54.806 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (139, 954306, 75, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 114, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (115.55, 67.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 49, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 191) [M::mem_pestat] mean and std.dev: (42.69, 25.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 245) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 224, 672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1700) [M::mem_pestat] mean and std.dev: (394.69, 350.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2214) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1238.792 CPU sec, 75.565 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (183, 954216, 75, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (121.55, 62.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 55, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (54.02, 39.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 386) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 489, 870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2216) [M::mem_pestat] mean and std.dev: (579.22, 534.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2889) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1200.952 CPU sec, 53.036 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (153, 954111, 100, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 130, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (121.39, 67.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 51, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (44.04, 32.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 224, 689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1791) [M::mem_pestat] mean and std.dev: (385.50, 392.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2342) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1222.224 CPU sec, 73.434 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (177, 953555, 84, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 138, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (131.08, 68.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 60, 1589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4705) [M::mem_pestat] mean and std.dev: (865.07, 1484.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6805) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 263, 1036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2800) [M::mem_pestat] mean and std.dev: (532.66, 556.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3682) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1206.235 CPU sec, 53.074 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (159, 954096, 78, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 125, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (127.16, 67.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 53, 75) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 155) [M::mem_pestat] mean and std.dev: (45.60, 19.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 195) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 202, 680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1700) [M::mem_pestat] mean and std.dev: (324.33, 309.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2210) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1210.345 CPU sec, 58.004 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (163, 954751, 100, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 111, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (114.11, 64.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 57, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (52.15, 34.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 288, 1232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3242) [M::mem_pestat] mean and std.dev: (687.05, 628.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4247) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1238.031 CPU sec, 75.809 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (162, 954314, 85, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 131, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (124.39, 66.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 60, 1331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3929) [M::mem_pestat] mean and std.dev: (414.04, 868.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5228) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 243, 873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2279) [M::mem_pestat] mean and std.dev: (463.59, 459.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2982) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1204.339 CPU sec, 53.620 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (166, 954808, 86, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 126, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (126.04, 79.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 67, 1364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4012) [M::mem_pestat] mean and std.dev: (341.03, 774.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5336) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 234, 683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1713) [M::mem_pestat] mean and std.dev: (383.10, 325.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2228) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1232.111 CPU sec, 75.075 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (150, 954152, 89, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 130, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (125.64, 73.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 52, 89) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 205) [M::mem_pestat] mean and std.dev: (45.47, 26.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 263) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 209, 810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2142) [M::mem_pestat] mean and std.dev: (450.66, 512.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2808) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1206.144 CPU sec, 53.102 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (164, 954345, 86, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 133, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (126.18, 71.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 55, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 175) [M::mem_pestat] mean and std.dev: (49.71, 25.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 221) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 238, 1122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3094) [M::mem_pestat] mean and std.dev: (575.37, 645.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4080) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1221.702 CPU sec, 72.959 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (156, 954917, 93, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 137, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (135.89, 69.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.74, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 58, 1202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3532) [M::mem_pestat] mean and std.dev: (393.63, 767.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4697) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (128, 225, 617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1595) [M::mem_pestat] mean and std.dev: (376.00, 359.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2084) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1195.686 CPU sec, 52.693 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (151, 954666, 98, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 137, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (131.56, 68.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 51, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (58.88, 66.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1008) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 251, 962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2510) [M::mem_pestat] mean and std.dev: (557.49, 516.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3284) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1236.462 CPU sec, 74.035 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (166, 955015, 88, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 130, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (125.90, 66.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 54, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 239) [M::mem_pestat] mean and std.dev: (54.66, 40.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 308) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 214, 1004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2710) [M::mem_pestat] mean and std.dev: (488.00, 533.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3563) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1204.348 CPU sec, 54.906 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (164, 953696, 58, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 128, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (125.19, 70.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 66, 920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2694) [M::mem_pestat] mean and std.dev: (255.43, 544.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3581) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 303, 1232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3254) [M::mem_pestat] mean and std.dev: (604.61, 628.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4265) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1235.972 CPU sec, 74.533 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (180, 954675, 96, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 143, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (143.88, 83.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 54, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (57.21, 59.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 245, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1289) [M::mem_pestat] mean and std.dev: (313.91, 182.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1650) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1200.584 CPU sec, 53.216 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (162, 954297, 86, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 117, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (119.51, 75.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 49, 1395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4125) [M::mem_pestat] mean and std.dev: (499.08, 1078.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5490) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 208, 703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1815) [M::mem_pestat] mean and std.dev: (421.07, 430.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2371) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1218.335 CPU sec, 72.164 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (188, 953629, 89, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 134, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (126.71, 68.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 58, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 899) [M::mem_pestat] mean and std.dev: (57.74, 59.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1187) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 213, 852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2198) [M::mem_pestat] mean and std.dev: (448.32, 501.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2871) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1204.809 CPU sec, 53.081 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (167, 954112, 101, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 139, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (131.08, 64.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 50, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (53.38, 50.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 483, 969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2473) [M::mem_pestat] mean and std.dev: (599.79, 501.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3225) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1211.024 CPU sec, 61.183 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (181, 953734, 98, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 132, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (124.64, 65.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 55, 743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2179) [M::mem_pestat] mean and std.dev: (155.06, 379.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2897) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 495, 1011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2595) [M::mem_pestat] mean and std.dev: (640.74, 593.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3387) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1239.813 CPU sec, 76.290 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (168, 953352, 91, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 128, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (122.41, 62.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 48, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (55.12, 59.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 325, 1204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3208) [M::mem_pestat] mean and std.dev: (580.28, 556.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4210) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1192.040 CPU sec, 52.657 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (163, 954262, 95, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 129, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (133.04, 76.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 59, 666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1914) [M::mem_pestat] mean and std.dev: (149.43, 289.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2538) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 286, 1619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4479) [M::mem_pestat] mean and std.dev: (976.33, 1206.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5909) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1219.585 CPU sec, 73.729 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (147, 954261, 82, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 113, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (118.58, 68.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 63, 1627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4805) [M::mem_pestat] mean and std.dev: (828.99, 1469.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6709) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 222, 1032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2830) [M::mem_pestat] mean and std.dev: (476.30, 479.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3729) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1200.140 CPU sec, 52.919 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (170, 953548, 84, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 146, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (137.74, 78.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 54, 1162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3422) [M::mem_pestat] mean and std.dev: (421.45, 882.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4552) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (103, 175, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (148.69, 83.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1224.836 CPU sec, 74.193 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (169, 954093, 90, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 136, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (126.00, 60.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 53, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (44.72, 25.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 684, 1336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3560) [M::mem_pestat] mean and std.dev: (743.91, 668.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4672) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1201.154 CPU sec, 52.874 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (154, 953909, 78, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 126, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (131.31, 72.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 54, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (72.59, 90.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 721) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 232, 669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1611) [M::mem_pestat] mean and std.dev: (384.68, 347.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2082) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1229.639 CPU sec, 73.608 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (166, 954798, 86, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 137, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (132.36, 64.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.87, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 57, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (61.96, 64.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 261, 1372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3772) [M::mem_pestat] mean and std.dev: (704.64, 780.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4972) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1212.021 CPU sec, 55.177 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (155, 954701, 93, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 121, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (120.95, 69.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 64, 655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1901) [M::mem_pestat] mean and std.dev: (137.13, 280.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2524) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 229, 1428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3924) [M::mem_pestat] mean and std.dev: (603.74, 757.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5172) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1233.482 CPU sec, 73.485 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (184, 954666, 92, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 129, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (123.11, 66.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.93) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 57, 2054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6094) [M::mem_pestat] mean and std.dev: (877.34, 1554.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8114) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 258, 763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1939) [M::mem_pestat] mean and std.dev: (438.45, 416.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1205.132 CPU sec, 53.166 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 954198, 77, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 134, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (128.86, 68.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 46, 78) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 186) [M::mem_pestat] mean and std.dev: (40.35, 25.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 240) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 273, 876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2180) [M::mem_pestat] mean and std.dev: (442.92, 355.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2832) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1210.996 CPU sec, 74.674 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (162, 953603, 92, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 129, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (127.75, 70.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 60, 913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2661) [M::mem_pestat] mean and std.dev: (209.73, 486.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3535) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 619, 1499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4133) [M::mem_pestat] mean and std.dev: (865.88, 955.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5450) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1193.204 CPU sec, 52.561 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (166, 954614, 96, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 123, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (121.78, 71.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 59, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (54.59, 40.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 228, 1174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3192) [M::mem_pestat] mean and std.dev: (546.69, 638.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4201) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1232.606 CPU sec, 65.531 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (150, 954247, 92, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 113, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (114.00, 62.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 57, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 578) [M::mem_pestat] mean and std.dev: (53.37, 44.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 316, 1248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3324) [M::mem_pestat] mean and std.dev: (657.28, 695.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4362) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1212.912 CPU sec, 73.669 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (170, 954906, 88, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 135, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (124.80, 67.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.70, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 49, 89) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 205) [M::mem_pestat] mean and std.dev: (45.66, 31.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 263) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 281, 1245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3457) [M::mem_pestat] mean and std.dev: (638.40, 802.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4563) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1190.097 CPU sec, 52.418 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (169, 954344, 81, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 131, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (128.82, 73.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 60, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (61.33, 54.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 243, 604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1442) [M::mem_pestat] mean and std.dev: (324.59, 331.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1861) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1237.619 CPU sec, 74.575 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (190, 954657, 65, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 118, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (122.07, 68.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 50, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (48.35, 32.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 249, 857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2239) [M::mem_pestat] mean and std.dev: (471.92, 446.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2930) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1199.292 CPU sec, 52.786 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (171, 953938, 73, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 126, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (127.81, 73.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 54, 92) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 204) [M::mem_pestat] mean and std.dev: (49.56, 25.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 260) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 253, 1022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2692) [M::mem_pestat] mean and std.dev: (499.33, 494.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1219.575 CPU sec, 73.469 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (171, 954478, 84, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 118, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (123.28, 70.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 61, 3103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9249) [M::mem_pestat] mean and std.dev: (1462.02, 2384.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12322) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 246, 949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2495) [M::mem_pestat] mean and std.dev: (526.64, 597.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3268) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1209.695 CPU sec, 53.272 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (162, 954230, 109, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 137, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (131.40, 74.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.74, 31.91) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 56, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1511) [M::mem_pestat] mean and std.dev: (90.49, 164.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2004) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 207, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (194.03, 70.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1194.285 CPU sec, 52.544 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (153, 954089, 87, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 113, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (115.62, 64.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.87, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 50, 100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 230) [M::mem_pestat] mean and std.dev: (47.17, 24.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 295) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 215, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (203.26, 90.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1236.792 CPU sec, 73.930 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (156, 954378, 85, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 124, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (123.91, 62.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 59, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (66.32, 71.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 258, 1271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3425) [M::mem_pestat] mean and std.dev: (760.81, 843.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4502) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1197.922 CPU sec, 54.256 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (156, 954055, 99, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 133, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (126.17, 59.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 44, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 662) [M::mem_pestat] mean and std.dev: (81.38, 122.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 336, 985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2543) [M::mem_pestat] mean and std.dev: (574.96, 559.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3322) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1229.150 CPU sec, 74.272 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (151, 954321, 95, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 135, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (129.96, 65.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 60, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (74.32, 85.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (124, 261, 1094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3034) [M::mem_pestat] mean and std.dev: (627.14, 643.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4004) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1198.153 CPU sec, 52.939 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 954436, 89, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 131, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (137.44, 81.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.92) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 54, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1339) [M::mem_pestat] mean and std.dev: (100.27, 180.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1776) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 270, 1142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3018) [M::mem_pestat] mean and std.dev: (625.75, 622.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3956) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1230.665 CPU sec, 74.926 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (163, 954239, 84, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 126, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (128.51, 71.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 56, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (65.64, 84.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 286, 1165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3147) [M::mem_pestat] mean and std.dev: (561.71, 521.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4138) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1196.450 CPU sec, 52.760 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (146, 953949, 96, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 133, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (127.34, 67.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.93) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 48, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (47.51, 41.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 307, 927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2431) [M::mem_pestat] mean and std.dev: (529.50, 492.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3183) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1245.114 CPU sec, 74.480 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (153, 954569, 96, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 119, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (119.86, 67.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.73, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 58, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (51.51, 40.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 527, 1230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3334) [M::mem_pestat] mean and std.dev: (734.84, 728.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4386) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1203.282 CPU sec, 54.847 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (171, 954123, 90, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 126, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (128.26, 78.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 54, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (61.46, 94.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 251, 592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1424) [M::mem_pestat] mean and std.dev: (365.91, 274.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1840) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1232.478 CPU sec, 74.318 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (151, 954380, 85, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 109, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (116.10, 72.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.86, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 49, 82) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 190) [M::mem_pestat] mean and std.dev: (40.89, 21.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 244) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 738, 1254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3388) [M::mem_pestat] mean and std.dev: (739.72, 675.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4455) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1204.838 CPU sec, 53.231 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (165, 953630, 80, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 133, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (131.35, 70.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.86, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 49, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 236) [M::mem_pestat] mean and std.dev: (49.84, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 305) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 262, 816) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2062) [M::mem_pestat] mean and std.dev: (495.40, 515.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2685) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1235.971 CPU sec, 73.685 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (150, 954637, 83, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 141, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (132.13, 68.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 53, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (50.35, 30.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 256, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2036) [M::mem_pestat] mean and std.dev: (429.93, 438.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2657) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1209.021 CPU sec, 53.187 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (168, 954641, 96, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 130, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (123.46, 67.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.74, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 53, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (70.74, 86.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 700) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 286, 1007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2645) [M::mem_pestat] mean and std.dev: (547.10, 530.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3464) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1202.392 CPU sec, 56.384 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (180, 954124, 90, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 131, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (128.70, 76.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 50, 931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2727) [M::mem_pestat] mean and std.dev: (289.93, 673.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3625) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 225, 760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1972) [M::mem_pestat] mean and std.dev: (396.60, 397.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2578) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1231.308 CPU sec, 76.104 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (167, 954377, 71, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 141, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (129.52, 72.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 61, 1192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3498) [M::mem_pestat] mean and std.dev: (342.13, 757.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4651) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 706, 1392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3886) [M::mem_pestat] mean and std.dev: (732.62, 722.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5133) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1207.846 CPU sec, 53.697 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 953959, 88, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 128, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (132.27, 65.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 32.04) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 48, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 182) [M::mem_pestat] mean and std.dev: (46.11, 28.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 233) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 620, 925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2243) [M::mem_pestat] mean and std.dev: (582.94, 453.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2902) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1219.634 CPU sec, 73.725 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 954631, 77, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 128, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (123.89, 63.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.75, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 52, 619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1793) [M::mem_pestat] mean and std.dev: (108.34, 258.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2380) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 261, 1063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2843) [M::mem_pestat] mean and std.dev: (667.71, 691.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3733) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1196.393 CPU sec, 52.594 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (154, 953765, 70, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 119, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (121.80, 90.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 56, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (67.51, 81.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 266, 900) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2336) [M::mem_pestat] mean and std.dev: (460.33, 425.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3054) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1237.064 CPU sec, 75.966 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (168, 954323, 97, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 130, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (135.60, 81.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.88, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 59, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (55.83, 42.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 304, 876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2206) [M::mem_pestat] mean and std.dev: (589.38, 555.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2871) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1201.294 CPU sec, 52.848 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (159, 954845, 89, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 141, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (139.66, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 52, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 820) [M::mem_pestat] mean and std.dev: (63.66, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 255, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1176) [M::mem_pestat] mean and std.dev: (321.09, 253.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1510) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1242.308 CPU sec, 74.010 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (166, 954322, 88, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 140, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (130.25, 72.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 53, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (55.50, 45.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 229, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1446) [M::mem_pestat] mean and std.dev: (309.12, 274.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1877) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1205.726 CPU sec, 54.638 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (181, 955055, 84, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 138, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (129.50, 74.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 58, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1633) [M::mem_pestat] mean and std.dev: (112.15, 196.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2164) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 280, 725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1763) [M::mem_pestat] mean and std.dev: (516.19, 443.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2290) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1225.687 CPU sec, 71.289 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (173, 954397, 80, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 140, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (132.17, 69.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 52, 112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (45.21, 26.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 229, 895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2369) [M::mem_pestat] mean and std.dev: (522.82, 611.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3106) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1201.268 CPU sec, 53.003 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 954526, 82, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 131, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (133.95, 71.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 60, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 534) [M::mem_pestat] mean and std.dev: (69.20, 85.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 704) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 269, 841) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2187) [M::mem_pestat] mean and std.dev: (492.26, 502.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2860) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1236.531 CPU sec, 73.940 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (143, 954401, 82, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 114, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (121.91, 65.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 47, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 236) [M::mem_pestat] mean and std.dev: (44.30, 38.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 306) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 288, 1030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2650) [M::mem_pestat] mean and std.dev: (513.50, 468.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3460) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1202.283 CPU sec, 53.010 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (160, 954565, 85, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 130, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (124.28, 59.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.86, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 51, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (49.35, 37.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 362) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 521, 934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2472) [M::mem_pestat] mean and std.dev: (573.17, 469.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3241) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1214.072 CPU sec, 60.446 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (160, 954876, 97, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 132, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (129.13, 73.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 61, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (59.55, 55.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 245, 758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1940) [M::mem_pestat] mean and std.dev: (423.41, 362.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2531) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1229.982 CPU sec, 75.462 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (172, 954578, 82, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 130, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (130.40, 76.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 31.91) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 55, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (74.22, 90.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (120, 252, 751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2013) [M::mem_pestat] mean and std.dev: (428.21, 488.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2644) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1192.325 CPU sec, 52.746 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (165, 954225, 70, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 119, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (118.22, 62.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 56, 102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 246) [M::mem_pestat] mean and std.dev: (46.19, 27.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 318) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 247, 905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2419) [M::mem_pestat] mean and std.dev: (546.28, 631.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3176) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1234.830 CPU sec, 74.304 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (158, 954333, 74, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 146, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (130.83, 69.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 49, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 174) [M::mem_pestat] mean and std.dev: (44.93, 23.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 221) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 267, 1305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3617) [M::mem_pestat] mean and std.dev: (709.08, 794.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1201.221 CPU sec, 52.945 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (179, 954639, 85, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 127, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (115.69, 66.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 58, 81) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 161) [M::mem_pestat] mean and std.dev: (50.99, 19.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 201) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 293, 1121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3019) [M::mem_pestat] mean and std.dev: (669.26, 722.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3968) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1222.165 CPU sec, 71.787 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (164, 954157, 100, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 126, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (120.89, 67.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 50, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 204) [M::mem_pestat] mean and std.dev: (43.11, 24.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 261) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 271, 987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2611) [M::mem_pestat] mean and std.dev: (607.45, 581.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3423) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1194.715 CPU sec, 52.553 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (164, 954005, 70, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 134, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (128.72, 67.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 47, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 157) [M::mem_pestat] mean and std.dev: (42.40, 21.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 200) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 340, 1394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3758) [M::mem_pestat] mean and std.dev: (686.71, 656.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4940) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1247.908 CPU sec, 73.866 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (145, 953793, 89, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 130, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (128.05, 69.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 53, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (66.26, 83.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1234) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 281, 1190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3166) [M::mem_pestat] mean and std.dev: (558.85, 528.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4154) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1211.612 CPU sec, 54.863 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (165, 955185, 85, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 117, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (116.78, 65.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 61, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (56.90, 39.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 406) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 262, 645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1557) [M::mem_pestat] mean and std.dev: (389.58, 317.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2013) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1223.422 CPU sec, 72.764 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (146, 954478, 72, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 131, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (118.63, 65.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 53, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (57.37, 60.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 232, 1056) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2810) [M::mem_pestat] mean and std.dev: (581.41, 698.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3687) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1197.341 CPU sec, 52.909 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (160, 954675, 85, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 120, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (124.73, 72.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 55, 709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2061) [M::mem_pestat] mean and std.dev: (120.29, 288.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2737) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 245, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1391) [M::mem_pestat] mean and std.dev: (268.30, 217.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1794) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1219.669 CPU sec, 74.097 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (161, 954275, 78, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 119, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (121.79, 72.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 54, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (50.67, 41.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 234, 751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1931) [M::mem_pestat] mean and std.dev: (388.00, 425.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2521) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1193.982 CPU sec, 52.589 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (162, 953579, 103, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 115, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (115.22, 66.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 57, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (64.50, 73.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 271, 978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2588) [M::mem_pestat] mean and std.dev: (496.57, 455.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3393) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1200.149 CPU sec, 63.498 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (183, 954663, 86, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 118, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (124.65, 83.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 60, 1034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3028) [M::mem_pestat] mean and std.dev: (417.82, 716.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4025) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 549, 1398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3770) [M::mem_pestat] mean and std.dev: (798.51, 775.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4956) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1218.159 CPU sec, 73.183 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (176, 954607, 83, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 137, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (132.27, 68.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.86, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 52, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1542) [M::mem_pestat] mean and std.dev: (77.92, 148.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2045) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 378, 1082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2800) [M::mem_pestat] mean and std.dev: (566.75, 500.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3659) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1188.540 CPU sec, 52.265 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (171, 954876, 94, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 108, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (117.70, 71.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.75, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 55, 92) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 210) [M::mem_pestat] mean and std.dev: (47.09, 27.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 269) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 311, 1236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3376) [M::mem_pestat] mean and std.dev: (642.52, 662.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4446) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1203.235 CPU sec, 71.512 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (168, 954803, 78, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 136, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (132.81, 70.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 62, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 624) [M::mem_pestat] mean and std.dev: (70.98, 81.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 258, 743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1889) [M::mem_pestat] mean and std.dev: (410.97, 334.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2462) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1194.426 CPU sec, 52.609 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 954640, 68, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 122, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (122.60, 66.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.86, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 51, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (59.74, 59.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 285, 1580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4360) [M::mem_pestat] mean and std.dev: (802.77, 845.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5750) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1207.833 CPU sec, 73.174 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (139, 954320, 87, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 120, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (118.12, 61.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.73, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 64, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1113) [M::mem_pestat] mean and std.dev: (74.88, 86.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1469) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 266, 876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2328) [M::mem_pestat] mean and std.dev: (434.57, 401.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3054) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1189.740 CPU sec, 52.355 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (184, 954014, 87, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 133, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (126.88, 71.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 32.04) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 65, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (94.91, 124.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 287, 853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2185) [M::mem_pestat] mean and std.dev: (448.32, 441.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2851) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1173.746 CPU sec, 51.680 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (162, 954224, 77, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 127, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (121.51, 68.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 56, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1085) [M::mem_pestat] mean and std.dev: (77.88, 110.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1433) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 289, 1609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4419) [M::mem_pestat] mean and std.dev: (1001.98, 1122.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5824) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1237.335 CPU sec, 75.453 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (185, 954833, 90, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 129, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (124.02, 68.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 59, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (64.36, 100.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 317, 903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2333) [M::mem_pestat] mean and std.dev: (517.38, 446.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3048) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1190.268 CPU sec, 52.800 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (171, 953544, 69, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 131, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (132.54, 70.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 58, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1209) [M::mem_pestat] mean and std.dev: (100.59, 170.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1600) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 263, 1185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3189) [M::mem_pestat] mean and std.dev: (641.74, 646.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1219.222 CPU sec, 72.641 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 954539, 84, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 123, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (124.19, 70.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 61, 109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 255) [M::mem_pestat] mean and std.dev: (56.69, 40.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 328) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 255, 1077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2839) [M::mem_pestat] mean and std.dev: (600.38, 606.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3720) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1184.924 CPU sec, 52.181 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (153, 954447, 94, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 131, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (130.32, 75.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 60, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 198) [M::mem_pestat] mean and std.dev: (53.13, 30.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 252) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 239, 882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2358) [M::mem_pestat] mean and std.dev: (412.23, 456.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3096) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1209.126 CPU sec, 71.644 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (131, 955135, 85, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 142, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (137.53, 68.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 60, 1546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4556) [M::mem_pestat] mean and std.dev: (921.96, 1606.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7350) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 242, 1004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2704) [M::mem_pestat] mean and std.dev: (523.14, 577.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3554) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1198.009 CPU sec, 52.732 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (154, 954099, 99, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 129, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (119.08, 65.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 54, 718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2082) [M::mem_pestat] mean and std.dev: (182.63, 421.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2764) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 266, 1211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3265) [M::mem_pestat] mean and std.dev: (720.64, 775.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4292) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1244.382 CPU sec, 73.960 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (165, 953556, 82, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 116, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (122.57, 68.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 60, 1575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4655) [M::mem_pestat] mean and std.dev: (911.88, 1609.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7348) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 618, 1376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3734) [M::mem_pestat] mean and std.dev: (824.72, 818.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4913) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1197.329 CPU sec, 54.407 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (163, 954256, 91, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 125, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (135.45, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.71, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 70, 1975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5853) [M::mem_pestat] mean and std.dev: (944.06, 1547.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7792) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 396, 847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2161) [M::mem_pestat] mean and std.dev: (522.62, 463.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2818) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1208.532 CPU sec, 71.395 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (177, 954076, 73, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 134, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (131.22, 69.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 51, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (67.80, 85.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 265, 657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1699) [M::mem_pestat] mean and std.dev: (433.91, 435.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2220) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1185.932 CPU sec, 52.435 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (172, 954048, 83, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 120, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (121.58, 69.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 55, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (52.94, 41.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 229, 650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1594) [M::mem_pestat] mean and std.dev: (379.27, 316.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2066) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1212.820 CPU sec, 73.355 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (158, 954694, 78, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 119, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (117.02, 61.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 57, 749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2185) [M::mem_pestat] mean and std.dev: (162.56, 333.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2903) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 691, 1274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3466) [M::mem_pestat] mean and std.dev: (770.66, 781.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4562) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1183.322 CPU sec, 52.093 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (176, 955065, 91, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 136, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (132.04, 70.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 50, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (49.70, 37.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 262, 891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2395) [M::mem_pestat] mean and std.dev: (495.88, 555.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3147) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1186.475 CPU sec, 54.357 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (152, 954873, 83, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 128, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (123.86, 68.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 59, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (65.38, 80.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 271, 1202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3246) [M::mem_pestat] mean and std.dev: (698.62, 779.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4268) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1246.806 CPU sec, 74.002 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (173, 953926, 92, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 131, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (125.08, 62.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 60, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 572) [M::mem_pestat] mean and std.dev: (76.05, 88.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 639, 1342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3512) [M::mem_pestat] mean and std.dev: (847.71, 790.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4597) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1199.273 CPU sec, 54.481 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (175, 954656, 86, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 124, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (121.74, 61.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 51, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 181) [M::mem_pestat] mean and std.dev: (48.13, 30.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 230) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 250, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021) [M::mem_pestat] mean and std.dev: (259.81, 183.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1304) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1205.482 CPU sec, 71.669 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (138, 954534, 92, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 124, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (125.50, 68.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 62, 2449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7265) [M::mem_pestat] mean and std.dev: (1001.12, 1764.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9673) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 266, 924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2472) [M::mem_pestat] mean and std.dev: (563.85, 553.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3246) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1191.757 CPU sec, 52.687 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (176, 954770, 75, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 135, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (122.91, 70.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 55, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (67.49, 93.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 235, 1032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2802) [M::mem_pestat] mean and std.dev: (520.91, 605.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3687) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1204.702 CPU sec, 72.648 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (154, 954512, 86, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 121, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (128.35, 73.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.87, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 59, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1229) [M::mem_pestat] mean and std.dev: (79.33, 154.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1627) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 225, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1345) [M::mem_pestat] mean and std.dev: (316.65, 312.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1742) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1190.992 CPU sec, 52.498 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 954217, 64, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 129, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (127.26, 72.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 57, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (63.02, 68.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 278, 1024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2680) [M::mem_pestat] mean and std.dev: (590.43, 521.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3508) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.609 CPU sec, 73.171 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (154, 954029, 88, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 143, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (138.87, 81.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 54, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (57.90, 52.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 295, 786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2022) [M::mem_pestat] mean and std.dev: (481.96, 450.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2640) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1202.858 CPU sec, 54.711 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (151, 953978, 85, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 117, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (123.71, 61.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 58, 1564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4614) [M::mem_pestat] mean and std.dev: (804.31, 1359.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6244) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (130, 236, 1047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2881) [M::mem_pestat] mean and std.dev: (528.36, 613.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3798) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1196.469 CPU sec, 71.730 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (159, 953942, 84, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 118, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (116.28, 67.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 52, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (89.16, 144.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 224, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1298) [M::mem_pestat] mean and std.dev: (284.62, 269.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1669) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1192.353 CPU sec, 52.643 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (201, 954366, 87, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 134, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (127.05, 66.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.86, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 55, 1283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3779) [M::mem_pestat] mean and std.dev: (386.35, 897.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5027) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 269, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1790) [M::mem_pestat] mean and std.dev: (365.00, 290.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2329) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1204.578 CPU sec, 70.805 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (179, 954562, 86, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 135, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (126.88, 74.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 56, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (50.73, 34.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 242, 654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1622) [M::mem_pestat] mean and std.dev: (366.09, 337.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2106) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1182.572 CPU sec, 52.058 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (156, 954316, 89, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 135, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (130.54, 68.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 32.03) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 52, 74) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 164) [M::mem_pestat] mean and std.dev: (42.96, 22.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 209) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 218, 1486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4124) [M::mem_pestat] mean and std.dev: (697.50, 959.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5443) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1191.474 CPU sec, 57.917 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (172, 954785, 104, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 129, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (122.17, 66.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 32.05) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 58, 1136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3344) [M::mem_pestat] mean and std.dev: (300.31, 651.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4448) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 273, 862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2176) [M::mem_pestat] mean and std.dev: (525.65, 552.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2833) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1223.270 CPU sec, 75.530 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 954989, 84, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 137, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (132.55, 68.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.73, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 55, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (49.11, 37.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (124, 251, 777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2083) [M::mem_pestat] mean and std.dev: (499.28, 585.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2842) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1193.975 CPU sec, 52.957 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (172, 954031, 83, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 123, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (125.64, 74.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 48, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 222) [M::mem_pestat] mean and std.dev: (45.79, 33.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 286) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 235, 950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2536) [M::mem_pestat] mean and std.dev: (425.67, 400.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3329) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1205.805 CPU sec, 72.343 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 953844, 96, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 139, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (128.99, 71.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 60, 546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1574) [M::mem_pestat] mean and std.dev: (169.10, 332.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2088) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 233, 743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1875) [M::mem_pestat] mean and std.dev: (396.08, 423.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2441) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1184.699 CPU sec, 52.170 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (183, 953737, 79, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 129, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (121.15, 64.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 64, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (64.77, 52.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 227, 625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1559) [M::mem_pestat] mean and std.dev: (295.63, 289.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2026) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1217.111 CPU sec, 74.253 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (175, 954396, 79, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 135, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (136.96, 75.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 56, 825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2405) [M::mem_pestat] mean and std.dev: (163.14, 340.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3195) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 246, 826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2160) [M::mem_pestat] mean and std.dev: (470.61, 483.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2827) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1193.433 CPU sec, 52.513 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (161, 954591, 91, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 123, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (122.45, 65.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 52, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (50.23, 39.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 242, 912) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2382) [M::mem_pestat] mean and std.dev: (474.03, 472.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3117) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1245.648 CPU sec, 73.889 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (165, 953825, 84, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 114, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (112.12, 64.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 53, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (62.38, 62.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 222, 722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1798) [M::mem_pestat] mean and std.dev: (366.57, 364.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2336) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1199.967 CPU sec, 54.374 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (145, 954176, 86, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 115, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (121.21, 68.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.91) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 61, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (56.33, 46.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 232, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1402) [M::mem_pestat] mean and std.dev: (310.10, 237.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1808) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1216.091 CPU sec, 72.466 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (166, 954653, 80, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 122, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (123.06, 66.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 48, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (48.51, 34.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 319, 1031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2743) [M::mem_pestat] mean and std.dev: (493.66, 471.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3599) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1195.223 CPU sec, 52.733 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (152, 953904, 80, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 119, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (123.28, 76.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.75, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 53, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (54.92, 49.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (121, 235, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1612) [M::mem_pestat] mean and std.dev: (353.95, 352.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2109) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1214.839 CPU sec, 73.144 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (162, 953664, 84, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 123, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (121.95, 74.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 62, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 880) [M::mem_pestat] mean and std.dev: (91.62, 120.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 235, 754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1944) [M::mem_pestat] mean and std.dev: (475.46, 461.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2539) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1189.744 CPU sec, 52.406 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 954629, 97, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 121, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (121.02, 62.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 58, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (68.24, 65.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 233, 995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2653) [M::mem_pestat] mean and std.dev: (537.34, 569.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3482) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1195.822 CPU sec, 61.826 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (162, 953497, 76, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 125, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (121.76, 67.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 53, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (62.27, 63.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 256, 1195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3215) [M::mem_pestat] mean and std.dev: (633.14, 693.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4225) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1232.562 CPU sec, 75.707 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (168, 953708, 79, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 130, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (130.31, 66.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 61, 735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2119) [M::mem_pestat] mean and std.dev: (148.97, 364.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2811) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 409, 1118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3004) [M::mem_pestat] mean and std.dev: (609.06, 548.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3947) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1202.450 CPU sec, 53.048 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (165, 954087, 81, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 135, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (131.81, 64.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 43, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 160) [M::mem_pestat] mean and std.dev: (41.81, 22.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 204) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 281, 1150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3076) [M::mem_pestat] mean and std.dev: (642.67, 623.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4039) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1201.538 CPU sec, 69.758 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (183, 954046, 84, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 120, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (124.06, 66.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 55, 942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2762) [M::mem_pestat] mean and std.dev: (214.38, 556.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3672) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 260, 776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1956) [M::mem_pestat] mean and std.dev: (421.16, 456.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2546) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1193.064 CPU sec, 52.648 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 954244, 73, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 134, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (128.48, 66.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 54, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 184) [M::mem_pestat] mean and std.dev: (45.61, 25.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 234) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 255, 848) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2204) [M::mem_pestat] mean and std.dev: (445.20, 445.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2882) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1206.967 CPU sec, 71.706 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 954859, 94, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 122, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (118.11, 68.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 31.92) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 56, 1156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3392) [M::mem_pestat] mean and std.dev: (310.81, 720.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4510) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 219, 1164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3146) [M::mem_pestat] mean and std.dev: (534.22, 641.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4137) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1188.799 CPU sec, 52.272 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (158, 954232, 98, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 147, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (138.33, 74.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 51, 86) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 196) [M::mem_pestat] mean and std.dev: (44.92, 23.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 251) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 263, 1080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2878) [M::mem_pestat] mean and std.dev: (583.03, 585.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3777) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.314 CPU sec, 73.673 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (166, 955275, 62, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 123, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (124.12, 68.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 45, 92) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 218) [M::mem_pestat] mean and std.dev: (40.27, 20.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 281) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 238, 1044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2842) [M::mem_pestat] mean and std.dev: (592.35, 656.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3741) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1211.544 CPU sec, 55.234 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (151, 954183, 80, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 117, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (121.09, 73.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.86, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 52, 1261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3719) [M::mem_pestat] mean and std.dev: (293.23, 669.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4948) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 308, 954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2426) [M::mem_pestat] mean and std.dev: (533.86, 497.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3162) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1195.692 CPU sec, 71.423 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 953707, 88, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 119, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (119.85, 67.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 47, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (56.88, 60.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 206, 759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1985) [M::mem_pestat] mean and std.dev: (402.58, 427.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2598) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1191.024 CPU sec, 52.689 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (145, 954640, 96, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 144, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (136.24, 65.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 56, 2123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6313) [M::mem_pestat] mean and std.dev: (864.03, 1502.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8408) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 700, 1023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2669) [M::mem_pestat] mean and std.dev: (574.27, 430.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3492) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1208.633 CPU sec, 72.483 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (167, 954317, 86, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 134, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (127.51, 66.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 53, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 153) [M::mem_pestat] mean and std.dev: (44.04, 18.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 193) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 235, 868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2288) [M::mem_pestat] mean and std.dev: (433.03, 480.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2998) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1185.071 CPU sec, 52.176 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (173, 954248, 73, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 136, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (133.91, 63.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 55, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1559) [M::mem_pestat] mean and std.dev: (70.81, 115.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2067) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 245, 882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2360) [M::mem_pestat] mean and std.dev: (461.31, 421.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3099) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1199.353 CPU sec, 65.732 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (172, 953886, 65, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 131, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (130.29, 61.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.86, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 55, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (54.63, 44.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (127, 227, 678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1780) [M::mem_pestat] mean and std.dev: (400.38, 411.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2331) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1236.907 CPU sec, 73.564 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 952839, 97, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 117, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (117.05, 60.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.72, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 52, 75) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 165) [M::mem_pestat] mean and std.dev: (46.01, 26.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 210) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 241, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1569) [M::mem_pestat] mean and std.dev: (357.29, 332.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2034) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1204.159 CPU sec, 53.146 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (168, 953639, 84, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 136, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (127.60, 63.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 63, 874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2540) [M::mem_pestat] mean and std.dev: (191.38, 453.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3373) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 224, 827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2197) [M::mem_pestat] mean and std.dev: (519.52, 620.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3003) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1242.653 CPU sec, 75.668 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (143, 953671, 80, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 131, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (124.53, 67.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 50, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (55.38, 51.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 216, 594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1418) [M::mem_pestat] mean and std.dev: (332.20, 304.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1830) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1203.556 CPU sec, 52.963 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (174, 954115, 86, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 120, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (122.91, 68.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 52, 865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2533) [M::mem_pestat] mean and std.dev: (145.84, 331.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3367) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 434, 970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2614) [M::mem_pestat] mean and std.dev: (627.28, 651.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3436) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1213.098 CPU sec, 71.388 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (158, 953681, 79, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 133, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (122.62, 71.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 51, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1404) [M::mem_pestat] mean and std.dev: (79.44, 173.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1860) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 231, 793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2071) [M::mem_pestat] mean and std.dev: (351.38, 334.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2710) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1195.740 CPU sec, 52.651 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (151, 954472, 86, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 133, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (124.96, 68.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 60, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (67.28, 69.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 405, 1135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3025) [M::mem_pestat] mean and std.dev: (554.00, 456.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3970) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1177.006 CPU sec, 52.262 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (158, 954466, 83, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 127, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (126.28, 74.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 57, 600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1738) [M::mem_pestat] mean and std.dev: (141.09, 296.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2307) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 237, 674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1660) [M::mem_pestat] mean and std.dev: (403.55, 382.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2153) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1243.987 CPU sec, 74.244 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (158, 954535, 89, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 131, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (121.91, 70.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 51, 636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1844) [M::mem_pestat] mean and std.dev: (108.28, 228.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2448) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 262, 986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2562) [M::mem_pestat] mean and std.dev: (581.07, 588.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3350) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1192.972 CPU sec, 54.356 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (155, 954893, 70, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 140, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (136.84, 71.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 47, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (53.59, 44.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 238, 959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2565) [M::mem_pestat] mean and std.dev: (449.05, 424.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3368) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1209.254 CPU sec, 72.697 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (165, 954197, 72, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 133, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (136.66, 70.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 58, 826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2404) [M::mem_pestat] mean and std.dev: (204.54, 461.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3193) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (126, 185, 568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1452) [M::mem_pestat] mean and std.dev: (297.00, 277.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1894) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1197.314 CPU sec, 52.991 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (152, 954603, 71, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 124, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (127.39, 67.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 70, 1494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4390) [M::mem_pestat] mean and std.dev: (551.25, 996.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5838) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (121, 235, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1216) [M::mem_pestat] mean and std.dev: (296.25, 254.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1581) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1210.187 CPU sec, 72.617 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (148, 953857, 91, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 134, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (131.42, 73.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 51, 86) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 208) [M::mem_pestat] mean and std.dev: (44.58, 27.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 269) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 276, 948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2504) [M::mem_pestat] mean and std.dev: (597.97, 605.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3282) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1200.102 CPU sec, 52.851 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (176, 953922, 86, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 118, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (125.47, 70.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 52, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (54.31, 43.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 232, 1037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2799) [M::mem_pestat] mean and std.dev: (594.94, 653.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3680) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1242.661 CPU sec, 73.437 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (160, 954574, 83, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 111, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (118.82, 70.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.93) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 58, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (65.51, 64.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (128, 229, 1590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4514) [M::mem_pestat] mean and std.dev: (607.19, 729.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5976) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1217.966 CPU sec, 55.471 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (179, 954112, 82, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 124, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (122.10, 64.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 51, 2060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6120) [M::mem_pestat] mean and std.dev: (951.72, 1580.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8150) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 275, 989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2573) [M::mem_pestat] mean and std.dev: (544.13, 487.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3365) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1216.525 CPU sec, 72.841 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (173, 954711, 70, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 134, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (122.68, 64.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 60, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (59.36, 46.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 267, 1178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3172) [M::mem_pestat] mean and std.dev: (542.84, 595.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4169) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1192.637 CPU sec, 52.627 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (175, 954340, 88, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 115, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (118.63, 69.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.86, 31.92) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 61, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (65.66, 64.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 263, 1172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3168) [M::mem_pestat] mean and std.dev: (549.90, 602.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4166) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1212.682 CPU sec, 73.442 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (143, 954580, 89, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 129, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (122.87, 69.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 32.04) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 44, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 209) [M::mem_pestat] mean and std.dev: (44.42, 27.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 268) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 233, 846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2244) [M::mem_pestat] mean and std.dev: (403.51, 463.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2943) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1190.807 CPU sec, 52.411 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (172, 954811, 87, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 133, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (119.92, 60.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.87, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 53, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (59.34, 59.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 255, 777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1957) [M::mem_pestat] mean and std.dev: (522.15, 492.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2547) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1192.044 CPU sec, 56.777 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (181, 953887, 84, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 124, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (128.19, 75.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.72, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 52, 1165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3433) [M::mem_pestat] mean and std.dev: (264.83, 590.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4567) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 233, 860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2278) [M::mem_pestat] mean and std.dev: (478.47, 553.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2987) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1227.929 CPU sec, 74.854 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (162, 954534, 81, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 144, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (138.67, 71.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 52, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (55.13, 47.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 369) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 233, 829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2181) [M::mem_pestat] mean and std.dev: (343.25, 329.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2857) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1201.599 CPU sec, 53.184 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (171, 954575, 84, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 136, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (132.75, 68.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 48, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 191) [M::mem_pestat] mean and std.dev: (39.62, 19.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 245) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 262, 1024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2696) [M::mem_pestat] mean and std.dev: (566.50, 572.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3532) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1219.685 CPU sec, 72.990 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (161, 953685, 113, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 136, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (125.90, 64.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.88, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 49, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (66.58, 89.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (132, 245, 763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2025) [M::mem_pestat] mean and std.dev: (475.11, 537.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2656) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1203.314 CPU sec, 53.036 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (167, 955153, 82, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 128, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (132.74, 81.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 53, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1655) [M::mem_pestat] mean and std.dev: (127.37, 281.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2196) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 254, 746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1898) [M::mem_pestat] mean and std.dev: (467.48, 433.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2474) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1219.512 CPU sec, 72.546 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (169, 954353, 96, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 133, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (133.62, 74.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 32.03) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 55, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1205) [M::mem_pestat] mean and std.dev: (63.64, 67.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1595) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 234, 924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2464) [M::mem_pestat] mean and std.dev: (445.85, 426.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3234) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1199.665 CPU sec, 52.792 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (174, 954416, 92, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 120, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (119.48, 68.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 54, 1731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5133) [M::mem_pestat] mean and std.dev: (726.19, 1386.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6834) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 285, 828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2144) [M::mem_pestat] mean and std.dev: (475.34, 438.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2802) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1238.215 CPU sec, 73.985 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (146, 953674, 95, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 131, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (129.33, 82.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 47, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (48.50, 43.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 267, 810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2084) [M::mem_pestat] mean and std.dev: (517.44, 498.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2721) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1210.370 CPU sec, 54.951 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (148, 954317, 69, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 118, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (122.68, 67.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 65, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (65.61, 61.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 277, 1445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3957) [M::mem_pestat] mean and std.dev: (743.29, 716.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5213) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1217.626 CPU sec, 72.685 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (144, 954122, 83, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 111, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (117.22, 67.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 53, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 208) [M::mem_pestat] mean and std.dev: (46.84, 28.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 267) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 221, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1146) [M::mem_pestat] mean and std.dev: (287.31, 230.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1472) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1198.614 CPU sec, 53.030 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (178, 954312, 75, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 137, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (128.62, 71.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 67, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (74.57, 83.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 227, 1216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3336) [M::mem_pestat] mean and std.dev: (575.00, 618.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4396) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1217.381 CPU sec, 74.915 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (158, 954233, 76, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 124, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (122.94, 62.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 48, 86) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 208) [M::mem_pestat] mean and std.dev: (48.25, 38.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 269) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 235, 861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2269) [M::mem_pestat] mean and std.dev: (445.45, 515.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2973) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1188.693 CPU sec, 52.360 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (166, 954017, 78, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 122, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (122.87, 68.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.75, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 57, 97) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 227) [M::mem_pestat] mean and std.dev: (49.31, 29.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 292) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 249, 679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1671) [M::mem_pestat] mean and std.dev: (386.61, 345.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2167) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1204.194 CPU sec, 60.961 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (180, 954025, 77, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 119, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (125.27, 72.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 49, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1190) [M::mem_pestat] mean and std.dev: (76.39, 170.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1577) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 257, 770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2022) [M::mem_pestat] mean and std.dev: (463.49, 457.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2648) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1232.783 CPU sec, 74.841 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (175, 954585, 101, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 130, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (129.59, 72.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.75, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 54, 1928) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5726) [M::mem_pestat] mean and std.dev: (981.28, 1674.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7681) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 274, 867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2189) [M::mem_pestat] mean and std.dev: (416.31, 453.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2850) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1189.635 CPU sec, 52.413 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (177, 953707, 80, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 127, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (123.96, 68.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 54, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 234) [M::mem_pestat] mean and std.dev: (57.24, 44.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 299) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 230, 825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2159) [M::mem_pestat] mean and std.dev: (386.74, 369.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1219.336 CPU sec, 73.759 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 954734, 80, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 127, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (130.24, 72.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 54, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 189) [M::mem_pestat] mean and std.dev: (46.48, 24.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 242) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 278, 993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2591) [M::mem_pestat] mean and std.dev: (518.38, 502.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3390) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1194.011 CPU sec, 52.629 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (163, 954820, 87, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 120, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (123.55, 72.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 59, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1570) [M::mem_pestat] mean and std.dev: (131.27, 267.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2081) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 253, 1043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2749) [M::mem_pestat] mean and std.dev: (567.86, 580.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3602) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1224.710 CPU sec, 71.758 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (173, 954906, 105, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 127, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (129.80, 70.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 49, 77) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 165) [M::mem_pestat] mean and std.dev: (44.93, 24.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 209) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 259, 848) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2194) [M::mem_pestat] mean and std.dev: (499.03, 514.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2867) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1187.937 CPU sec, 52.318 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (138, 954402, 82, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 120, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (115.09, 62.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 54, 1412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4158) [M::mem_pestat] mean and std.dev: (304.47, 627.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5531) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 230, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1466) [M::mem_pestat] mean and std.dev: (357.10, 311.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1890) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1242.660 CPU sec, 73.817 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (169, 954538, 82, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 127, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (123.42, 61.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 60, 790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2288) [M::mem_pestat] mean and std.dev: (260.81, 509.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3037) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 269, 802) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2038) [M::mem_pestat] mean and std.dev: (429.30, 384.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2656) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1202.524 CPU sec, 54.717 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (159, 954407, 98, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 128, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (126.71, 73.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.87, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 66, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (92.28, 115.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 244, 850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2222) [M::mem_pestat] mean and std.dev: (448.42, 458.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2908) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1223.241 CPU sec, 73.400 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 953941, 85, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 121, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (124.12, 71.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 56, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 233) [M::mem_pestat] mean and std.dev: (47.41, 25.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 299) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (106, 259, 739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2005) [M::mem_pestat] mean and std.dev: (524.38, 531.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2650) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1193.417 CPU sec, 52.663 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (167, 954339, 81, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 119, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (125.48, 67.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 58, 1395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4117) [M::mem_pestat] mean and std.dev: (316.44, 689.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5478) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (124, 249, 800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2152) [M::mem_pestat] mean and std.dev: (452.43, 454.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2828) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1218.115 CPU sec, 73.139 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (192, 953829, 83, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 134, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (128.61, 73.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 46, 77) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 169) [M::mem_pestat] mean and std.dev: (42.59, 26.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 215) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 291, 977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2515) [M::mem_pestat] mean and std.dev: (485.69, 391.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3284) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1206.085 CPU sec, 53.233 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (161, 954257, 81, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 133, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (122.45, 67.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 61, 1083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3173) [M::mem_pestat] mean and std.dev: (381.25, 745.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4218) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 279, 1181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3147) [M::mem_pestat] mean and std.dev: (701.56, 736.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4130) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1207.525 CPU sec, 64.439 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (167, 954382, 64, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 140, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (138.39, 74.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.75, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 53, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1106) [M::mem_pestat] mean and std.dev: (76.98, 132.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1462) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 244, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1297) [M::mem_pestat] mean and std.dev: (344.81, 281.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1670) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1227.151 CPU sec, 73.341 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (159, 954606, 84, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 122, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (125.78, 68.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 55, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (54.00, 48.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 341, 1404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3760) [M::mem_pestat] mean and std.dev: (636.36, 540.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4938) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1200.025 CPU sec, 52.764 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (151, 954718, 82, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 135, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (125.58, 64.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 63, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (67.78, 77.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 246, 846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2204) [M::mem_pestat] mean and std.dev: (337.83, 333.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2883) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1224.387 CPU sec, 75.005 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (160, 954185, 79, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 128, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (125.37, 68.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 56, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1302) [M::mem_pestat] mean and std.dev: (129.89, 258.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1724) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 247, 894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2416) [M::mem_pestat] mean and std.dev: (453.94, 447.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3177) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1204.086 CPU sec, 52.959 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (160, 954262, 93, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (89, 134, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (145.57, 88.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.72, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 48, 725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2113) [M::mem_pestat] mean and std.dev: (165.83, 391.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2807) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 626, 1724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4812) [M::mem_pestat] mean and std.dev: (811.33, 758.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6356) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1221.523 CPU sec, 71.504 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (138, 954319, 86, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 126, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (129.77, 68.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 60, 633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1821) [M::mem_pestat] mean and std.dev: (132.68, 259.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2415) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 221, 973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2643) [M::mem_pestat] mean and std.dev: (428.06, 521.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3478) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1201.143 CPU sec, 52.925 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (164, 954083, 81, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 136, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (125.76, 70.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 50, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 217) [M::mem_pestat] mean and std.dev: (47.76, 35.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 280) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 237, 887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2367) [M::mem_pestat] mean and std.dev: (433.57, 482.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3107) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1186.244 CPU sec, 52.308 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (182, 953438, 62, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 117, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (123.88, 76.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 48, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 149) [M::mem_pestat] mean and std.dev: (41.29, 22.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 189) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 657, 1114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2960) [M::mem_pestat] mean and std.dev: (572.08, 475.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3883) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1237.485 CPU sec, 75.698 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (169, 954194, 96, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 119, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (124.27, 69.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.93) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 53, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (45.12, 22.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 357) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 210, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1334) [M::mem_pestat] mean and std.dev: (316.00, 260.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1721) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1204.781 CPU sec, 53.467 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (152, 954567, 87, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 144, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (136.69, 65.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 49, 78) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 174) [M::mem_pestat] mean and std.dev: (44.86, 27.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 222) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 222, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1574) [M::mem_pestat] mean and std.dev: (409.44, 387.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2053) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1219.661 CPU sec, 74.583 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (165, 954334, 78, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 130, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (126.27, 70.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 54, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 206) [M::mem_pestat] mean and std.dev: (48.45, 30.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 264) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 689, 1388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3710) [M::mem_pestat] mean and std.dev: (842.80, 833.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4871) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1186.566 CPU sec, 52.242 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (171, 954471, 96, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 128, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (121.71, 65.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.71, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 60, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (67.40, 73.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 231, 693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1789) [M::mem_pestat] mean and std.dev: (318.62, 325.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2337) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1220.502 CPU sec, 73.831 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (143, 955254, 86, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 132, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (125.74, 63.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 63, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1764) [M::mem_pestat] mean and std.dev: (84.70, 173.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2338) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 242, 767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1997) [M::mem_pestat] mean and std.dev: (420.52, 433.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2612) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1195.589 CPU sec, 52.617 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (167, 954522, 76, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 113, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (120.64, 70.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.74, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 51, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (89.39, 154.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1210) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 290, 627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1445) [M::mem_pestat] mean and std.dev: (365.50, 269.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1854) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1245.001 CPU sec, 74.256 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (152, 953589, 93, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 118, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (120.60, 60.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 67, 612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1762) [M::mem_pestat] mean and std.dev: (121.44, 266.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2337) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 248, 972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2644) [M::mem_pestat] mean and std.dev: (462.54, 464.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3480) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1214.406 CPU sec, 55.183 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (156, 952809, 87, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 108, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (111.41, 63.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 53, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 209) [M::mem_pestat] mean and std.dev: (46.94, 28.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 267) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 231, 757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1987) [M::mem_pestat] mean and std.dev: (383.11, 354.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2602) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1222.267 CPU sec, 72.932 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 954602, 89, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 123, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (117.61, 64.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 52, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (78.56, 110.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1249) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 282, 1029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2645) [M::mem_pestat] mean and std.dev: (642.04, 606.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3453) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1179.091 CPU sec, 52.039 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (158, 954093, 97, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 159, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (145.53, 72.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 56, 581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1671) [M::mem_pestat] mean and std.dev: (111.17, 229.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2216) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 323, 1038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2820) [M::mem_pestat] mean and std.dev: (476.92, 404.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3711) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1220.968 CPU sec, 74.131 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (155, 954353, 82, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 142, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (136.01, 75.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 60, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 533) [M::mem_pestat] mean and std.dev: (58.52, 47.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 335, 978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2516) [M::mem_pestat] mean and std.dev: (566.16, 453.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3285) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1203.304 CPU sec, 53.171 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (134, 953843, 87, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 127, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (123.41, 69.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 60, 1783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5271) [M::mem_pestat] mean and std.dev: (907.74, 1547.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7097) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 247, 740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1830) [M::mem_pestat] mean and std.dev: (452.70, 445.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2375) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1201.751 CPU sec, 56.468 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (162, 955439, 86, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 131, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (130.93, 71.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 43, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 158) [M::mem_pestat] mean and std.dev: (41.41, 23.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 201) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 250, 999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2711) [M::mem_pestat] mean and std.dev: (597.22, 685.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3567) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1236.032 CPU sec, 75.966 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (145, 954121, 113, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 108, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (115.21, 65.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 55, 97) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 227) [M::mem_pestat] mean and std.dev: (50.60, 34.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 292) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 257, 1003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2687) [M::mem_pestat] mean and std.dev: (612.95, 595.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3529) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1209.324 CPU sec, 53.614 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (159, 954275, 79, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 130, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (135.99, 75.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 55, 79) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 181) [M::mem_pestat] mean and std.dev: (45.79, 25.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 232) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 225, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1385) [M::mem_pestat] mean and std.dev: (346.61, 359.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1795) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1215.327 CPU sec, 72.909 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (161, 954396, 74, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 123, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (124.70, 71.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.87, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 61, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (67.74, 65.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 387, 1062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2810) [M::mem_pestat] mean and std.dev: (597.76, 481.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3684) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1195.836 CPU sec, 52.753 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (169, 955190, 92, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 119, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (122.57, 77.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 54, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1538) [M::mem_pestat] mean and std.dev: (83.68, 169.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2040) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 219, 944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2484) [M::mem_pestat] mean and std.dev: (448.48, 491.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3254) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1221.602 CPU sec, 74.780 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (169, 954182, 76, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 126, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (124.99, 71.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 43, 74) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 176) [M::mem_pestat] mean and std.dev: (40.22, 25.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 227) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 537, 1737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4831) [M::mem_pestat] mean and std.dev: (868.71, 911.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6378) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1205.807 CPU sec, 53.128 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (154, 953889, 84, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 121, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (121.54, 72.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 54, 1258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3706) [M::mem_pestat] mean and std.dev: (380.52, 844.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4930) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 249, 850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2206) [M::mem_pestat] mean and std.dev: (497.56, 521.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2884) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1261.845 CPU sec, 74.787 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (158, 953838, 95, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 128, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (132.93, 73.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.73, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 55, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (62.64, 62.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 661, 1101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2941) [M::mem_pestat] mean and std.dev: (700.49, 719.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3861) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1216.251 CPU sec, 55.313 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (143, 954185, 87, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 123, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (123.36, 69.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 46, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 153) [M::mem_pestat] mean and std.dev: (41.79, 22.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 195) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 247, 975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2515) [M::mem_pestat] mean and std.dev: (483.27, 530.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3285) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1217.785 CPU sec, 72.772 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (148, 954051, 79, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 124, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (121.36, 70.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.75, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 66, 2045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6059) [M::mem_pestat] mean and std.dev: (884.80, 1517.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8066) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 266, 673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1721) [M::mem_pestat] mean and std.dev: (326.06, 255.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2245) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1200.153 CPU sec, 53.039 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (171, 954103, 86, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 129, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (127.25, 67.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.93) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 51, 95) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 215) [M::mem_pestat] mean and std.dev: (49.61, 29.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 275) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 234, 768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1960) [M::mem_pestat] mean and std.dev: (377.74, 396.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2556) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1196.115 CPU sec, 70.783 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (162, 953804, 82, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 114, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (116.03, 65.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 32.06) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 57, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (54.18, 47.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 275, 1234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3370) [M::mem_pestat] mean and std.dev: (677.41, 766.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4438) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1190.259 CPU sec, 52.573 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (151, 954637, 97, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 128, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (123.21, 66.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.93) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 60, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 247) [M::mem_pestat] mean and std.dev: (48.53, 29.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 318) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 623, 1043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2645) [M::mem_pestat] mean and std.dev: (686.60, 574.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3446) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1197.327 CPU sec, 60.246 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (155, 954522, 88, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 123, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (123.27, 73.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 32.03) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 57, 1389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4095) [M::mem_pestat] mean and std.dev: (279.79, 707.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5448) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 231, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1477) [M::mem_pestat] mean and std.dev: (336.24, 323.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1923) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1220.543 CPU sec, 75.177 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (167, 954907, 97, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 129, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (125.93, 64.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 53, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (65.11, 97.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1240) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 248, 896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2336) [M::mem_pestat] mean and std.dev: (525.70, 576.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3056) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1189.690 CPU sec, 52.876 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (163, 954310, 73, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 128, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (127.46, 69.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 53, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (62.02, 67.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 237, 739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1893) [M::mem_pestat] mean and std.dev: (397.90, 378.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2470) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1207.609 CPU sec, 72.773 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (187, 954783, 76, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 128, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (129.23, 75.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 47, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (43.63, 33.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (127, 235, 883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2395) [M::mem_pestat] mean and std.dev: (450.75, 470.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3151) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1187.460 CPU sec, 52.291 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (179, 954935, 87, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 135, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (126.76, 66.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 56, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1414) [M::mem_pestat] mean and std.dev: (99.80, 194.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1875) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 250, 1019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2711) [M::mem_pestat] mean and std.dev: (528.77, 540.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3557) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1206.179 CPU sec, 70.020 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (182, 954578, 70, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 134, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (122.43, 67.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 51, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 252) [M::mem_pestat] mean and std.dev: (43.46, 24.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 326) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 234, 875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2265) [M::mem_pestat] mean and std.dev: (457.24, 480.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2960) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1195.966 CPU sec, 52.730 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (152, 954936, 103, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 121, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (121.36, 57.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 49, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (53.26, 48.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 733, 1177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3105) [M::mem_pestat] mean and std.dev: (732.80, 647.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4069) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1237.225 CPU sec, 73.509 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (138, 954012, 79, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 135, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (129.35, 68.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 61, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 557) [M::mem_pestat] mean and std.dev: (65.60, 69.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 235, 1090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2914) [M::mem_pestat] mean and std.dev: (477.61, 484.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1189.789 CPU sec, 54.253 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (155, 953576, 79, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 118, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (120.74, 65.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 32.05) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 56, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (74.06, 84.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1067) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 448, 1401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3819) [M::mem_pestat] mean and std.dev: (662.82, 653.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5028) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1210.365 CPU sec, 72.420 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (163, 954909, 80, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 122, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (125.93, 71.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 50, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 156) [M::mem_pestat] mean and std.dev: (43.26, 23.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 198) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 272, 857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2197) [M::mem_pestat] mean and std.dev: (407.46, 408.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2867) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1190.178 CPU sec, 52.607 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (142, 954637, 82, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 153, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (141.29, 75.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 61, 740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2150) [M::mem_pestat] mean and std.dev: (118.26, 261.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2855) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 234, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1411) [M::mem_pestat] mean and std.dev: (381.43, 332.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1824) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1193.794 CPU sec, 71.050 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (181, 954538, 76, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 131, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (125.60, 67.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 48, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (50.10, 48.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 237, 647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1643) [M::mem_pestat] mean and std.dev: (321.58, 317.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2141) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1184.161 CPU sec, 52.145 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (145, 954368, 72, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 120, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (120.65, 67.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 60, 792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2294) [M::mem_pestat] mean and std.dev: (228.72, 499.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3045) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 606, 1106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2868) [M::mem_pestat] mean and std.dev: (733.29, 607.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3749) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1190.937 CPU sec, 63.387 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (164, 954612, 87, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 123, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (120.49, 62.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 59, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047) [M::mem_pestat] mean and std.dev: (99.53, 187.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1386) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (117, 221, 685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1821) [M::mem_pestat] mean and std.dev: (339.00, 367.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2389) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1210.027 CPU sec, 72.648 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (176, 954625, 97, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 145, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (135.59, 72.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 50, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (52.08, 37.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 673, 1531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4209) [M::mem_pestat] mean and std.dev: (869.66, 766.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5548) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1186.251 CPU sec, 52.195 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (182, 954195, 96, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 120, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (119.99, 66.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 32.03) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 54, 89) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 205) [M::mem_pestat] mean and std.dev: (49.96, 34.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 263) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 257, 959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2555) [M::mem_pestat] mean and std.dev: (467.00, 574.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3353) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1196.798 CPU sec, 71.272 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (141, 954202, 88, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 113, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (125.36, 71.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 58, 2024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6010) [M::mem_pestat] mean and std.dev: (1036.74, 1660.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8003) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (124, 208, 771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2065) [M::mem_pestat] mean and std.dev: (401.54, 471.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2712) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1183.427 CPU sec, 52.075 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (170, 954837, 74, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 138, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (133.20, 71.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 57, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (48.60, 36.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 231, 787) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2021) [M::mem_pestat] mean and std.dev: (385.03, 385.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2638) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1203.639 CPU sec, 73.065 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (160, 954937, 96, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 134, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (132.12, 73.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 55, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012) [M::mem_pestat] mean and std.dev: (74.00, 115.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1340) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 275, 870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2290) [M::mem_pestat] mean and std.dev: (476.62, 474.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3000) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1183.061 CPU sec, 52.038 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (155, 954204, 84, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 131, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (124.94, 61.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 62, 1328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3908) [M::mem_pestat] mean and std.dev: (464.60, 961.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5198) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 262, 886) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2288) [M::mem_pestat] mean and std.dev: (509.40, 520.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2989) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1180.233 CPU sec, 52.043 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (164, 954203, 90, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 125, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (129.92, 69.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 60, 2077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6157) [M::mem_pestat] mean and std.dev: (1074.56, 1704.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8197) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 248, 1174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3202) [M::mem_pestat] mean and std.dev: (564.12, 587.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4216) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1198.507 CPU sec, 74.528 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (169, 954030, 84, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 133, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (130.39, 74.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 65, 1826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5410) [M::mem_pestat] mean and std.dev: (883.73, 1421.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7202) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 224, 860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2252) [M::mem_pestat] mean and std.dev: (430.32, 506.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2948) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1193.134 CPU sec, 52.705 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (164, 954327, 87, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 134, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (135.72, 68.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 55, 1867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5521) [M::mem_pestat] mean and std.dev: (655.73, 1188.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7348) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 280, 933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2469) [M::mem_pestat] mean and std.dev: (592.73, 601.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3237) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1194.230 CPU sec, 70.895 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (153, 954849, 85, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 139, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (136.74, 70.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 66, 1424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4196) [M::mem_pestat] mean and std.dev: (495.17, 885.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5582) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 221, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (226.31, 125.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1196.489 CPU sec, 52.792 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (138, 954400, 74, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 136, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (128.35, 64.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.74, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 55, 1548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4568) [M::mem_pestat] mean and std.dev: (808.94, 1428.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6522) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (124, 209, 784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2104) [M::mem_pestat] mean and std.dev: (331.69, 351.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2764) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1197.530 CPU sec, 71.264 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (169, 954624, 71, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 122, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (121.34, 66.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 32.03) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 48, 70) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 146) [M::mem_pestat] mean and std.dev: (43.79, 18.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 184) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 290, 895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2267) [M::mem_pestat] mean and std.dev: (492.61, 459.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2953) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1189.313 CPU sec, 52.352 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (154, 953825, 83, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 143, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (128.49, 71.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 32.04) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 58, 85) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 185) [M::mem_pestat] mean and std.dev: (47.85, 24.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 235) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 899, 1516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4076) [M::mem_pestat] mean and std.dev: (909.54, 809.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5356) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1238.836 CPU sec, 73.533 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (161, 954324, 80, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 129, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (128.20, 70.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 55, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 210) [M::mem_pestat] mean and std.dev: (50.44, 33.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 268) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 279, 990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2592) [M::mem_pestat] mean and std.dev: (565.42, 588.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3393) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1198.559 CPU sec, 54.429 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (153, 954274, 90, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 120, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (118.11, 64.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 50, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (70.51, 89.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 255, 934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2486) [M::mem_pestat] mean and std.dev: (523.26, 549.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3262) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1210.748 CPU sec, 71.597 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (178, 954844, 72, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 128, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (123.29, 72.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 54, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (67.69, 83.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 235, 701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1729) [M::mem_pestat] mean and std.dev: (380.06, 414.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2243) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1187.866 CPU sec, 52.445 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (150, 954309, 77, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 124, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (121.33, 67.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 55, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (47.92, 30.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 257, 1014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2732) [M::mem_pestat] mean and std.dev: (566.08, 517.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3591) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1221.412 CPU sec, 73.889 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (145, 954111, 74, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 117, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (117.11, 65.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 59, 992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2908) [M::mem_pestat] mean and std.dev: (232.69, 548.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3866) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 249, 1010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2692) [M::mem_pestat] mean and std.dev: (579.23, 610.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3533) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1185.047 CPU sec, 52.159 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (177, 954335, 84, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 141, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (132.51, 67.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 55, 1507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4455) [M::mem_pestat] mean and std.dev: (447.33, 952.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5929) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 276, 1021) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2667) [M::mem_pestat] mean and std.dev: (567.10, 547.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3490) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1182.048 CPU sec, 54.100 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (183, 953745, 94, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 137, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (133.90, 75.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 49, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (56.53, 60.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 213, 1006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2724) [M::mem_pestat] mean and std.dev: (557.97, 673.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3583) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1239.136 CPU sec, 74.276 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 953846, 98, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 119, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (112.42, 63.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 55, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249) [M::mem_pestat] mean and std.dev: (56.90, 47.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 321) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 246, 1022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2726) [M::mem_pestat] mean and std.dev: (611.84, 731.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3578) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1195.689 CPU sec, 54.418 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (173, 954706, 84, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 127, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (129.90, 65.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.90) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 55, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (50.89, 37.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 249, 625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1513) [M::mem_pestat] mean and std.dev: (383.13, 363.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1957) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1207.365 CPU sec, 71.930 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (159, 954513, 77, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 117, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (121.15, 72.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.86, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 54, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 267) [M::mem_pestat] mean and std.dev: (55.95, 48.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 345) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 267, 931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2367) [M::mem_pestat] mean and std.dev: (558.85, 507.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3085) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1193.364 CPU sec, 52.810 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (145, 954458, 74, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 139, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (130.67, 63.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.86, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 60, 1176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3464) [M::mem_pestat] mean and std.dev: (311.13, 703.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4608) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 272, 704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1732) [M::mem_pestat] mean and std.dev: (406.06, 345.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2246) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1214.530 CPU sec, 74.380 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (169, 954432, 77, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 124, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (123.30, 68.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 60, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (78.32, 98.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 857) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 250, 972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2564) [M::mem_pestat] mean and std.dev: (600.70, 631.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3360) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1187.219 CPU sec, 52.283 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (147, 953984, 79, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 115, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (122.23, 75.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 59, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (60.07, 61.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1205) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 261, 934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2490) [M::mem_pestat] mean and std.dev: (463.74, 476.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3268) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1251.404 CPU sec, 74.478 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 954594, 66, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 129, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (124.98, 62.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 49, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 147) [M::mem_pestat] mean and std.dev: (46.71, 21.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 184) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 263, 1151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3061) [M::mem_pestat] mean and std.dev: (660.20, 691.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4016) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1198.121 CPU sec, 54.467 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (158, 954241, 77, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 102, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (116.34, 72.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 47, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (53.56, 50.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 670, 1268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3374) [M::mem_pestat] mean and std.dev: (795.49, 732.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4427) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1213.210 CPU sec, 73.122 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (160, 953526, 81, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 142, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (133.36, 68.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 60, 2095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6213) [M::mem_pestat] mean and std.dev: (1036.44, 1718.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8272) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 227, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1244) [M::mem_pestat] mean and std.dev: (286.32, 219.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1603) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1188.817 CPU sec, 52.493 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (162, 954875, 91, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 110, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (117.49, 66.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 64, 2014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5968) [M::mem_pestat] mean and std.dev: (931.20, 1673.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7945) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 260, 739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1857) [M::mem_pestat] mean and std.dev: (462.52, 420.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2416) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1202.612 CPU sec, 71.458 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (183, 953950, 76, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 125, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (123.80, 71.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.71, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 47, 119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 293) [M::mem_pestat] mean and std.dev: (51.39, 41.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 224, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1529) [M::mem_pestat] mean and std.dev: (366.05, 367.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1986) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1196.809 CPU sec, 52.813 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (152, 953649, 78, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (122.68, 65.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 44, 70) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 152) [M::mem_pestat] mean and std.dev: (38.33, 19.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 193) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 224, 638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1552) [M::mem_pestat] mean and std.dev: (290.62, 233.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2009) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1185.897 CPU sec, 58.001 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (144, 954129, 74, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 126, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (124.66, 69.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 44, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (68.62, 132.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1040) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 253, 721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1811) [M::mem_pestat] mean and std.dev: (392.07, 322.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2356) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1223.948 CPU sec, 75.454 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (160, 954457, 76, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 129, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (128.40, 71.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 61, 779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2251) [M::mem_pestat] mean and std.dev: (170.21, 399.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2987) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 646, 1462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4060) [M::mem_pestat] mean and std.dev: (847.79, 859.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5359) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1191.339 CPU sec, 52.885 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (156, 954349, 66, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 124, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (123.65, 72.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 56, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (72.22, 100.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1073) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 243, 885) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2325) [M::mem_pestat] mean and std.dev: (443.37, 429.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3045) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1204.845 CPU sec, 73.033 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (169, 953825, 92, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 134, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (134.42, 75.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 70, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (97.78, 143.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1248) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 293, 1017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2613) [M::mem_pestat] mean and std.dev: (616.24, 650.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3411) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1188.751 CPU sec, 52.383 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (166, 954699, 76, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 138, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (135.65, 73.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 52, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1438) [M::mem_pestat] mean and std.dev: (120.95, 249.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1908) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (116, 208, 658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1742) [M::mem_pestat] mean and std.dev: (358.06, 396.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2284) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1193.896 CPU sec, 73.475 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (168, 953911, 80, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 132, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (129.80, 74.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 58, 1492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4418) [M::mem_pestat] mean and std.dev: (539.69, 1000.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5881) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 619, 1613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4405) [M::mem_pestat] mean and std.dev: (913.65, 948.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5801) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1197.297 CPU sec, 52.660 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (143, 954108, 106, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 125, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (128.17, 63.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 53, 1544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4564) [M::mem_pestat] mean and std.dev: (857.88, 1551.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7063) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 216, 617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1559) [M::mem_pestat] mean and std.dev: (293.41, 239.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2030) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1245.302 CPU sec, 74.085 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (163, 954049, 84, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 124, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (122.20, 65.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 48, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (56.30, 60.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 279, 1187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3213) [M::mem_pestat] mean and std.dev: (544.28, 625.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4226) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1198.874 CPU sec, 54.438 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (166, 954807, 93, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 136, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (128.78, 73.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 48, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 289) [M::mem_pestat] mean and std.dev: (52.19, 42.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (113, 205, 1091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3047) [M::mem_pestat] mean and std.dev: (361.33, 470.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4025) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1203.809 CPU sec, 72.471 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (176, 953977, 76, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 138, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (128.46, 67.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 49, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 205) [M::mem_pestat] mean and std.dev: (49.68, 30.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 262) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 312, 1067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2879) [M::mem_pestat] mean and std.dev: (637.25, 672.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3785) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1189.664 CPU sec, 52.587 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (152, 954112, 86, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 148, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (133.06, 68.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 46, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (50.02, 44.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 321, 1135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3075) [M::mem_pestat] mean and std.dev: (646.91, 738.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4045) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1203.016 CPU sec, 71.910 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (162, 953718, 87, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 135, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (128.51, 66.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.75, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 53, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1423) [M::mem_pestat] mean and std.dev: (105.96, 191.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1887) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (127, 203, 582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1492) [M::mem_pestat] mean and std.dev: (275.55, 220.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1947) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1185.791 CPU sec, 52.217 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (145, 954621, 94, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 121, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (129.51, 69.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.74, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 51, 74) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 158) [M::mem_pestat] mean and std.dev: (43.36, 24.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 200) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 245, 1025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2675) [M::mem_pestat] mean and std.dev: (511.61, 470.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3500) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1210.511 CPU sec, 62.768 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (166, 954187, 93, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 138, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (131.03, 68.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.73, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 50, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (50.56, 60.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 254, 1237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3297) [M::mem_pestat] mean and std.dev: (670.21, 774.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4327) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1220.492 CPU sec, 73.262 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (168, 954179, 88, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 122, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (128.99, 73.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.72, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 49, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (58.99, 61.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 270, 863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2207) [M::mem_pestat] mean and std.dev: (475.50, 466.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2879) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1191.512 CPU sec, 52.585 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 954517, 93, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 134, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (127.89, 72.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 59, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1501) [M::mem_pestat] mean and std.dev: (133.97, 278.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1989) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 263, 911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2451) [M::mem_pestat] mean and std.dev: (469.12, 423.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3221) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1202.788 CPU sec, 71.663 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (177, 954629, 75, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 129, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (123.23, 67.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 50, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 247) [M::mem_pestat] mean and std.dev: (43.90, 26.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 320) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 272, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2563) [M::mem_pestat] mean and std.dev: (488.81, 561.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3362) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1191.323 CPU sec, 52.565 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (169, 953749, 78, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 122, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (122.88, 71.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 51, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (61.38, 65.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 222, 1001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2607) [M::mem_pestat] mean and std.dev: (566.14, 598.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3410) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1202.874 CPU sec, 71.676 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (170, 953697, 87, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 127, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (130.93, 71.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 50, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (57.28, 69.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 285, 1056) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2766) [M::mem_pestat] mean and std.dev: (547.25, 488.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3621) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1195.565 CPU sec, 52.720 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 954532, 72, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 135, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (132.76, 69.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 57, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1070) [M::mem_pestat] mean and std.dev: (82.93, 156.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1415) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 258, 750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1910) [M::mem_pestat] mean and std.dev: (448.68, 472.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2490) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1237.679 CPU sec, 73.952 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 953523, 88, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 127, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (127.11, 74.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 55, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (65.65, 72.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 873) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 229, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1566) [M::mem_pestat] mean and std.dev: (368.37, 378.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2036) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1200.789 CPU sec, 54.492 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (155, 954424, 80, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 134, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (126.01, 66.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.74, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 55, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (48.48, 33.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 382) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 210, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1066) [M::mem_pestat] mean and std.dev: (236.03, 180.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1371) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1200.668 CPU sec, 70.370 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (165, 953694, 85, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 127, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (117.97, 63.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.74, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 66, 1615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4773) [M::mem_pestat] mean and std.dev: (601.83, 1225.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6352) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 225, 680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1620) [M::mem_pestat] mean and std.dev: (385.84, 365.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2090) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1188.664 CPU sec, 52.404 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (172, 954444, 98, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 131, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (128.01, 71.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 55, 85) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 187) [M::mem_pestat] mean and std.dev: (49.33, 31.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 238) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 259, 853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2189) [M::mem_pestat] mean and std.dev: (475.98, 425.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2857) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1206.974 CPU sec, 72.837 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (155, 954607, 89, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 137, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (133.29, 71.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 46, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 224) [M::mem_pestat] mean and std.dev: (42.64, 24.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 289) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 229, 693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1761) [M::mem_pestat] mean and std.dev: (346.22, 345.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2295) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1198.229 CPU sec, 53.040 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (147, 953754, 93, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 128, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (125.41, 73.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 56, 783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2281) [M::mem_pestat] mean and std.dev: (163.79, 345.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3030) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 214, 840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2154) [M::mem_pestat] mean and std.dev: (400.71, 431.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2811) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1202.958 CPU sec, 66.100 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (161, 954647, 99, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 124, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (124.69, 61.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.93) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 47, 81) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 187) [M::mem_pestat] mean and std.dev: (43.34, 25.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 240) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 272, 857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2167) [M::mem_pestat] mean and std.dev: (490.27, 485.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2822) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1222.070 CPU sec, 74.028 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (160, 954237, 71, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 127, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (121.52, 67.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 56, 108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 256) [M::mem_pestat] mean and std.dev: (51.30, 38.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 315, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3087) [M::mem_pestat] mean and std.dev: (628.11, 561.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4052) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1198.329 CPU sec, 52.878 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (129, 953882, 85, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 123, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (117.26, 57.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 50, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (52.54, 44.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 251, 775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1943) [M::mem_pestat] mean and std.dev: (334.12, 277.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1202.485 CPU sec, 67.878 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (155, 954679, 90, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 137, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (133.39, 70.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 61, 108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (49.77, 31.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 342) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 307, 753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1861) [M::mem_pestat] mean and std.dev: (452.57, 364.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2415) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1192.074 CPU sec, 52.602 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (152, 954256, 73, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 144, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (136.20, 69.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 50, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (52.12, 59.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 874) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 247, 741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1871) [M::mem_pestat] mean and std.dev: (411.09, 424.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2436) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1208.861 CPU sec, 72.534 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 954420, 82, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 115, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (115.25, 63.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.87, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 52, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (45.60, 29.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 401) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 229, 768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2000) [M::mem_pestat] mean and std.dev: (478.32, 475.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2616) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1199.191 CPU sec, 52.926 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (170, 953696, 91, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 128, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (130.17, 73.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 63, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (55.52, 47.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 294, 921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2399) [M::mem_pestat] mean and std.dev: (523.40, 465.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3138) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1178.796 CPU sec, 52.646 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (151, 953840, 92, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 114, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (121.74, 64.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 53, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (61.84, 69.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 405, 1172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3220) [M::mem_pestat] mean and std.dev: (645.90, 557.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4244) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1248.153 CPU sec, 74.424 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (151, 954313, 92, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 121, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (117.19, 61.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 53, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1295) [M::mem_pestat] mean and std.dev: (64.96, 92.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1715) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 204, 640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1616) [M::mem_pestat] mean and std.dev: (311.45, 299.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2104) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1199.065 CPU sec, 54.258 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (169, 953778, 83, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 122, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (122.15, 70.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 54, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 831) [M::mem_pestat] mean and std.dev: (71.62, 116.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1097) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 564, 1086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2798) [M::mem_pestat] mean and std.dev: (726.59, 670.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3654) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1207.792 CPU sec, 71.505 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (155, 954661, 79, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 138, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (131.91, 67.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 59, 2235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6633) [M::mem_pestat] mean and std.dev: (1081.06, 1723.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8832) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 271, 894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2336) [M::mem_pestat] mean and std.dev: (473.86, 508.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3057) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1197.538 CPU sec, 52.905 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (187, 953568, 81, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 118, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (120.19, 68.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 47, 77) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 169) [M::mem_pestat] mean and std.dev: (40.62, 22.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 215) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 544, 1605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4477) [M::mem_pestat] mean and std.dev: (830.03, 881.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5913) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1216.990 CPU sec, 71.511 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (145, 954302, 88, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 126, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (123.77, 62.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 59, 779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2277) [M::mem_pestat] mean and std.dev: (252.55, 530.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3026) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 241, 893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2341) [M::mem_pestat] mean and std.dev: (402.45, 380.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3065) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1193.853 CPU sec, 52.618 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (159, 954655, 66, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 135, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (134.53, 73.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.74, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 66, 2707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8037) [M::mem_pestat] mean and std.dev: (1234.02, 1908.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10702) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 310, 1258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3372) [M::mem_pestat] mean and std.dev: (718.10, 738.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4429) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1243.097 CPU sec, 73.662 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (155, 954755, 85, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 129, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (122.52, 65.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 55, 1275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3755) [M::mem_pestat] mean and std.dev: (261.69, 579.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4995) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 680, 2091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5825) [M::mem_pestat] mean and std.dev: (1050.12, 1210.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7692) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1208.365 CPU sec, 55.059 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (160, 954324, 89, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 139, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (129.69, 66.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.86, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 53, 79) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 163) [M::mem_pestat] mean and std.dev: (46.07, 23.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 205) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 248, 1109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2983) [M::mem_pestat] mean and std.dev: (751.63, 833.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4085) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1212.978 CPU sec, 72.079 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (165, 954683, 83, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 126, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (124.12, 73.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 49, 75) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 153) [M::mem_pestat] mean and std.dev: (42.63, 20.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 192) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 227, 805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2053) [M::mem_pestat] mean and std.dev: (409.88, 412.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2677) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1195.807 CPU sec, 52.935 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (169, 953646, 80, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 120, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (120.88, 69.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 49, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (52.97, 60.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (132, 229, 686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1794) [M::mem_pestat] mean and std.dev: (291.20, 280.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2348) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1218.390 CPU sec, 73.706 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (154, 954619, 81, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 130, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (121.92, 64.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.75, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 61, 1966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5826) [M::mem_pestat] mean and std.dev: (962.83, 1570.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7756) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 252, 1019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2785) [M::mem_pestat] mean and std.dev: (515.03, 519.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3668) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1184.443 CPU sec, 52.140 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (166, 954823, 92, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 132, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (126.17, 73.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.86, 32.05) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 55, 1608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4758) [M::mem_pestat] mean and std.dev: (699.65, 1340.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6333) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 283, 1654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4614) [M::mem_pestat] mean and std.dev: (939.46, 1182.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6094) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1194.452 CPU sec, 56.913 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (134, 953770, 93, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 123, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (119.45, 60.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 52, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 229) [M::mem_pestat] mean and std.dev: (58.41, 43.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 293) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 250, 826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2140) [M::mem_pestat] mean and std.dev: (454.32, 445.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2797) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1227.477 CPU sec, 75.444 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (144, 954994, 79, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 128, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (124.13, 62.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 59, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (63.03, 67.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 286, 980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2528) [M::mem_pestat] mean and std.dev: (494.03, 418.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3302) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1191.882 CPU sec, 52.703 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (156, 954808, 87, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 143, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (130.15, 61.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.75, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 46, 67) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 147) [M::mem_pestat] mean and std.dev: (40.03, 20.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 187) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 308, 1031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2659) [M::mem_pestat] mean and std.dev: (642.20, 570.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3473) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1206.621 CPU sec, 72.081 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (164, 954502, 88, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 135, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (125.46, 67.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.86, 31.92) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 64, 937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2745) [M::mem_pestat] mean and std.dev: (246.67, 574.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3649) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 562, 1158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3056) [M::mem_pestat] mean and std.dev: (662.74, 490.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4005) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1194.906 CPU sec, 52.603 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (134, 954082, 71, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 126, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (127.15, 63.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 53, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (58.55, 45.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 260, 1379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3735) [M::mem_pestat] mean and std.dev: (749.97, 884.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4913) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1203.297 CPU sec, 72.008 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (158, 954187, 70, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 137, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (126.38, 67.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 54, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 271) [M::mem_pestat] mean and std.dev: (52.74, 40.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 238, 1083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2925) [M::mem_pestat] mean and std.dev: (543.22, 599.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3846) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1183.186 CPU sec, 51.988 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (177, 954668, 84, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 134, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (134.32, 76.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 54, 89) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 209) [M::mem_pestat] mean and std.dev: (47.00, 28.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 269) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 691, 1055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2721) [M::mem_pestat] mean and std.dev: (645.97, 516.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3554) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1242.494 CPU sec, 73.824 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (161, 953889, 90, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 128, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (124.18, 66.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 52, 89) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 213) [M::mem_pestat] mean and std.dev: (44.28, 34.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 275) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 680, 1175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3081) [M::mem_pestat] mean and std.dev: (619.18, 539.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4034) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1215.713 CPU sec, 55.209 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (176, 954540, 81, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 132, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (135.23, 79.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.88, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 47, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 184) [M::mem_pestat] mean and std.dev: (44.18, 25.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 234) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 247, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2301) [M::mem_pestat] mean and std.dev: (407.87, 436.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3011) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1228.972 CPU sec, 73.666 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (145, 954041, 73, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 120, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (119.94, 65.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 54, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (54.39, 49.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 233, 1009) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2751) [M::mem_pestat] mean and std.dev: (495.65, 557.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3622) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1201.127 CPU sec, 53.065 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (150, 954335, 87, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 132, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (129.55, 71.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 50, 1451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4287) [M::mem_pestat] mean and std.dev: (336.79, 723.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5705) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 258, 789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1975) [M::mem_pestat] mean and std.dev: (315.78, 220.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2568) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1222.248 CPU sec, 73.422 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (154, 953833, 88, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 112, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (118.11, 65.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 69, 2417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7155) [M::mem_pestat] mean and std.dev: (1105.58, 1766.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9524) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 237, 695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1707) [M::mem_pestat] mean and std.dev: (410.51, 404.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2213) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1203.841 CPU sec, 53.232 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (171, 954561, 91, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 129, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (127.74, 71.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 50, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 235) [M::mem_pestat] mean and std.dev: (52.66, 35.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 301) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 232, 902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2398) [M::mem_pestat] mean and std.dev: (493.63, 478.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3146) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1191.507 CPU sec, 60.380 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (171, 953887, 80, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 120, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (123.57, 68.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 32.04) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 49, 79) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 177) [M::mem_pestat] mean and std.dev: (47.57, 33.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 226) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 257, 653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1615) [M::mem_pestat] mean and std.dev: (343.70, 322.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2096) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1237.997 CPU sec, 75.176 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (147, 954550, 84, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 122, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (125.83, 65.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 57, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (58.03, 59.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 256, 761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1935) [M::mem_pestat] mean and std.dev: (446.19, 423.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2522) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1183.546 CPU sec, 52.097 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (153, 954498, 89, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 122, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (122.14, 67.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.75, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 59, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (66.29, 68.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 273, 1126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2992) [M::mem_pestat] mean and std.dev: (577.80, 567.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3925) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1204.673 CPU sec, 72.286 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (165, 954329, 80, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 113, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (120.21, 69.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 51, 77) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 177) [M::mem_pestat] mean and std.dev: (46.79, 30.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 227) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (112, 194, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1570) [M::mem_pestat] mean and std.dev: (324.56, 353.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2056) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1197.298 CPU sec, 52.668 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (176, 954642, 74, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 130, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (125.12, 70.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 32.03) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 47, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 161) [M::mem_pestat] mean and std.dev: (42.38, 21.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 205) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 279, 867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2165) [M::mem_pestat] mean and std.dev: (479.28, 364.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2814) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1212.783 CPU sec, 72.199 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (167, 954039, 97, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 117, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (116.42, 63.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 55, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 322) [M::mem_pestat] mean and std.dev: (49.74, 31.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 266, 1272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3438) [M::mem_pestat] mean and std.dev: (647.91, 672.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4521) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1199.471 CPU sec, 52.825 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (161, 954924, 91, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 131, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (127.03, 70.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 60, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1161) [M::mem_pestat] mean and std.dev: (92.32, 146.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1536) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 253, 985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2625) [M::mem_pestat] mean and std.dev: (551.38, 593.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3445) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.856 CPU sec, 73.537 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (154, 955127, 92, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 121, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (117.93, 74.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 50, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (59.11, 74.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (128, 217, 575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1469) [M::mem_pestat] mean and std.dev: (337.72, 352.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1916) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1193.988 CPU sec, 54.231 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (148, 953569, 85, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 132, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (128.74, 75.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 49, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (54.19, 59.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 245, 983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2605) [M::mem_pestat] mean and std.dev: (523.95, 558.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3416) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1220.505 CPU sec, 73.633 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (161, 954341, 73, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 124, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (121.71, 68.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 56, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1597) [M::mem_pestat] mean and std.dev: (67.68, 91.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2119) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 325, 890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2338) [M::mem_pestat] mean and std.dev: (545.24, 538.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3062) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1188.176 CPU sec, 52.685 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (154, 954697, 87, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 128, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (124.19, 67.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 53, 89) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 193) [M::mem_pestat] mean and std.dev: (45.68, 21.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 245) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (128, 205, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1271) [M::mem_pestat] mean and std.dev: (251.31, 244.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1652) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1221.773 CPU sec, 72.750 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 953864, 90, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 115, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (117.72, 66.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.86, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 51, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 160) [M::mem_pestat] mean and std.dev: (45.00, 22.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 202) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 248, 1209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3265) [M::mem_pestat] mean and std.dev: (525.15, 509.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4293) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1189.316 CPU sec, 52.365 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (158, 954378, 104, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 134, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (132.22, 67.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 61, 603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1735) [M::mem_pestat] mean and std.dev: (140.46, 297.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2301) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 342, 1056) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2762) [M::mem_pestat] mean and std.dev: (627.32, 543.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3615) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1211.691 CPU sec, 65.184 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 954222, 84, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 121, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (129.77, 76.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.74, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 52, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (64.22, 75.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 255, 813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2049) [M::mem_pestat] mean and std.dev: (458.27, 389.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2667) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1200.153 CPU sec, 63.157 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (147, 954038, 86, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 129, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (134.26, 73.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 53, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 253) [M::mem_pestat] mean and std.dev: (48.85, 35.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 261, 1341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3669) [M::mem_pestat] mean and std.dev: (607.59, 660.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4833) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1200.239 CPU sec, 52.751 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (154, 953240, 78, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 129, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (127.78, 65.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 61, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1185) [M::mem_pestat] mean and std.dev: (117.39, 217.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1567) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 286, 996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2572) [M::mem_pestat] mean and std.dev: (553.43, 538.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3360) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1214.990 CPU sec, 72.643 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (176, 953962, 85, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 139, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (131.05, 77.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.86, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 50, 1322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3898) [M::mem_pestat] mean and std.dev: (239.84, 557.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5186) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (123, 228, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1257) [M::mem_pestat] mean and std.dev: (265.31, 270.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1635) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1187.658 CPU sec, 52.246 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 954213, 73, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 126, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (123.30, 64.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 57, 909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2667) [M::mem_pestat] mean and std.dev: (235.16, 443.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3546) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 250, 677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1667) [M::mem_pestat] mean and std.dev: (352.97, 310.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2162) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1204.381 CPU sec, 72.619 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (145, 954303, 96, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 130, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (130.99, 74.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 31.91) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 56, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 209) [M::mem_pestat] mean and std.dev: (49.81, 30.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 268) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 229, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1569) [M::mem_pestat] mean and std.dev: (332.21, 332.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2034) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1199.209 CPU sec, 52.828 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (171, 954841, 68, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 118, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (120.12, 65.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 60, 1024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2982) [M::mem_pestat] mean and std.dev: (323.09, 720.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3961) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 254, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1371) [M::mem_pestat] mean and std.dev: (291.38, 226.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1765) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1174.702 CPU sec, 51.876 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (159, 954731, 81, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 119, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (121.11, 68.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 54, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (49.89, 44.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 298, 1038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2718) [M::mem_pestat] mean and std.dev: (623.50, 551.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3558) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1227.819 CPU sec, 76.084 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (162, 954209, 86, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 127, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (132.91, 79.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 49, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (46.71, 35.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 303, 1048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2820) [M::mem_pestat] mean and std.dev: (538.51, 523.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3706) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1193.941 CPU sec, 53.080 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (170, 954646, 83, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 114, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (117.96, 61.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 32.04) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 57, 690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2004) [M::mem_pestat] mean and std.dev: (168.71, 372.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2661) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 272, 1041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2745) [M::mem_pestat] mean and std.dev: (476.18, 458.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3597) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1218.927 CPU sec, 73.334 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (162, 954585, 81, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 119, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (113.36, 61.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.86, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 56, 86) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 188) [M::mem_pestat] mean and std.dev: (49.32, 25.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 239) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 301, 1027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2765) [M::mem_pestat] mean and std.dev: (615.08, 665.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3634) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1195.668 CPU sec, 52.614 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (167, 954078, 92, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 133, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (127.76, 66.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 58, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (58.49, 63.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 254, 1219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3339) [M::mem_pestat] mean and std.dev: (555.97, 603.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4399) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1218.267 CPU sec, 75.064 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (159, 954203, 75, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 137, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (129.51, 68.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 48, 75) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 171) [M::mem_pestat] mean and std.dev: (41.20, 24.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 219) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 487, 971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2491) [M::mem_pestat] mean and std.dev: (606.37, 540.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3251) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1204.241 CPU sec, 53.175 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (193, 953972, 80, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 130, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (123.90, 66.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 56, 815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2371) [M::mem_pestat] mean and std.dev: (114.10, 272.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3149) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 550, 1063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2807) [M::mem_pestat] mean and std.dev: (597.58, 543.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3679) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1251.689 CPU sec, 74.659 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (159, 955047, 80, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 128, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (134.76, 77.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.75, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 52, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (62.37, 63.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 262, 894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2298) [M::mem_pestat] mean and std.dev: (517.00, 466.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3000) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1201.449 CPU sec, 54.550 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (174, 954287, 79, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 143, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (133.77, 71.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 48, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 176) [M::mem_pestat] mean and std.dev: (44.44, 29.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 224) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 312, 937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2441) [M::mem_pestat] mean and std.dev: (519.12, 453.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3193) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1208.579 CPU sec, 72.252 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (181, 954040, 99, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 131, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (123.49, 75.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 61, 1100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3238) [M::mem_pestat] mean and std.dev: (372.36, 749.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4307) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 290, 1081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2881) [M::mem_pestat] mean and std.dev: (559.50, 533.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3781) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1201.810 CPU sec, 53.107 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (199, 955127, 103, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 127, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (117.08, 65.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 59, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (69.22, 92.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 657, 1282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3472) [M::mem_pestat] mean and std.dev: (865.32, 809.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4567) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1206.588 CPU sec, 72.239 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (164, 954426, 85, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 120, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (124.31, 63.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 50, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (52.09, 48.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 907) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 261, 687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1615) [M::mem_pestat] mean and std.dev: (361.48, 315.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2079) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1196.237 CPU sec, 52.830 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 954886, 71, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 118, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (124.97, 75.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 52, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 237) [M::mem_pestat] mean and std.dev: (48.30, 34.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 304) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 277, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1356) [M::mem_pestat] mean and std.dev: (296.64, 169.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1742) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1179.349 CPU sec, 55.622 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (178, 953834, 80, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 130, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (128.04, 71.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 47, 67) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 145) [M::mem_pestat] mean and std.dev: (39.55, 18.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 184) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 288, 1288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3498) [M::mem_pestat] mean and std.dev: (648.10, 609.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4603) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1230.305 CPU sec, 73.802 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (159, 954098, 90, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 140, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (134.99, 70.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 51, 85) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 183) [M::mem_pestat] mean and std.dev: (47.38, 26.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 232) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 232, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1414) [M::mem_pestat] mean and std.dev: (265.58, 194.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1822) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1195.037 CPU sec, 54.258 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (144, 954401, 95, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 131, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (126.41, 69.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 55, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 290) [M::mem_pestat] mean and std.dev: (55.91, 44.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 261, 993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2637) [M::mem_pestat] mean and std.dev: (619.51, 639.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3459) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1204.539 CPU sec, 72.058 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (143, 953553, 95, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 145, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (133.60, 74.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 50, 787) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2295) [M::mem_pestat] mean and std.dev: (158.51, 347.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3049) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 235, 791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2049) [M::mem_pestat] mean and std.dev: (468.35, 431.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2678) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1192.004 CPU sec, 52.634 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (121, 954413, 86, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 145, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (134.60, 64.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 57, 1042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3060) [M::mem_pestat] mean and std.dev: (307.19, 677.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4069) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 209, 657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1645) [M::mem_pestat] mean and std.dev: (375.67, 390.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2139) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1209.910 CPU sec, 72.802 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (146, 953861, 90, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 132, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (130.56, 73.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 60, 1772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5234) [M::mem_pestat] mean and std.dev: (847.10, 1480.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6965) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 273, 996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2588) [M::mem_pestat] mean and std.dev: (535.08, 528.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3384) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1203.443 CPU sec, 53.035 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (177, 955023, 72, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 145, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (132.78, 63.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 54, 82) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 184) [M::mem_pestat] mean and std.dev: (42.61, 19.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 235) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 236, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1596) [M::mem_pestat] mean and std.dev: (319.78, 286.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2070) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1239.093 CPU sec, 73.512 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (162, 953553, 94, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 125, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (120.30, 63.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 49, 946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2766) [M::mem_pestat] mean and std.dev: (184.87, 459.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3676) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 597, 1091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2863) [M::mem_pestat] mean and std.dev: (701.84, 589.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3749) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1198.814 CPU sec, 54.454 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (176, 954447, 93, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 124, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (124.10, 72.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 60, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1460) [M::mem_pestat] mean and std.dev: (96.60, 202.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1935) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 224, 759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1983) [M::mem_pestat] mean and std.dev: (481.12, 495.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2595) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1207.986 CPU sec, 72.034 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (158, 954137, 94, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 126, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (124.67, 69.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 50, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (39.79, 24.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 282, 1126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3046) [M::mem_pestat] mean and std.dev: (588.66, 586.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4006) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1205.083 CPU sec, 53.241 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (172, 953662, 78, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 139, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (127.66, 66.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 43, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 160) [M::mem_pestat] mean and std.dev: (41.68, 24.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 204) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 246, 980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2632) [M::mem_pestat] mean and std.dev: (452.45, 509.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3458) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1206.343 CPU sec, 72.422 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (176, 954110, 99, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 130, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (126.30, 64.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.86, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 57, 1106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3240) [M::mem_pestat] mean and std.dev: (278.83, 633.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4307) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 235, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1262) [M::mem_pestat] mean and std.dev: (286.85, 199.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1627) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1191.570 CPU sec, 52.554 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (137, 954438, 92, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 122, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (116.55, 68.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 55, 1244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3662) [M::mem_pestat] mean and std.dev: (369.01, 806.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4871) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 208, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (168.72, 66.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1184.900 CPU sec, 59.456 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (159, 954297, 96, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 131, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (131.23, 64.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 44, 79) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 181) [M::mem_pestat] mean and std.dev: (41.23, 25.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 232) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 256, 767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1947) [M::mem_pestat] mean and std.dev: (486.45, 477.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2537) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1244.102 CPU sec, 73.900 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (179, 954275, 80, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 116, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (125.66, 68.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 58, 81) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 175) [M::mem_pestat] mean and std.dev: (45.62, 23.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 222) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 221, 1315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3603) [M::mem_pestat] mean and std.dev: (594.30, 670.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4747) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1193.251 CPU sec, 54.321 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (178, 954690, 79, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 138, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (129.19, 74.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 47, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 226) [M::mem_pestat] mean and std.dev: (41.55, 24.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 292) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 555, 1093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2843) [M::mem_pestat] mean and std.dev: (656.55, 494.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3718) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1208.580 CPU sec, 72.118 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (155, 954925, 77, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 125, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (118.82, 63.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 53, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1351) [M::mem_pestat] mean and std.dev: (90.08, 153.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1790) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 242, 1087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2869) [M::mem_pestat] mean and std.dev: (582.46, 565.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3760) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1189.667 CPU sec, 52.498 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (168, 954786, 91, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 136, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (133.06, 64.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 58, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 245) [M::mem_pestat] mean and std.dev: (50.89, 28.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 314) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 790, 1087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2843) [M::mem_pestat] mean and std.dev: (727.68, 626.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3721) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1206.031 CPU sec, 71.329 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (160, 954068, 81, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 121, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (125.40, 68.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 48, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (53.03, 48.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 221, 1058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2868) [M::mem_pestat] mean and std.dev: (583.15, 640.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1197.154 CPU sec, 52.724 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (169, 953527, 90, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 122, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (125.14, 75.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 51, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 245) [M::mem_pestat] mean and std.dev: (48.82, 35.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 316) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (132, 249, 1073) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2955) [M::mem_pestat] mean and std.dev: (595.22, 673.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3896) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1243.011 CPU sec, 73.857 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (174, 953847, 78, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 121, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (126.48, 76.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 59, 688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1994) [M::mem_pestat] mean and std.dev: (157.70, 348.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2647) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 230, 818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2140) [M::mem_pestat] mean and std.dev: (410.12, 509.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2801) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1201.295 CPU sec, 54.803 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (166, 954119, 91, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 123, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (122.99, 69.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 47, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 143) [M::mem_pestat] mean and std.dev: (42.68, 25.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 180) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 233, 653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1643) [M::mem_pestat] mean and std.dev: (293.59, 246.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2138) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1205.338 CPU sec, 72.245 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 953620, 90, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 124, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (126.43, 70.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 55, 936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2736) [M::mem_pestat] mean and std.dev: (350.83, 776.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3636) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 288, 868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2178) [M::mem_pestat] mean and std.dev: (482.11, 443.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2833) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1203.533 CPU sec, 53.336 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (168, 954818, 98, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 128, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (130.79, 78.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.87, 32.03) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 46, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 164) [M::mem_pestat] mean and std.dev: (45.04, 27.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 208) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 292, 877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2285) [M::mem_pestat] mean and std.dev: (522.85, 524.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2989) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1209.396 CPU sec, 71.776 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (160, 954876, 84, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 129, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (122.38, 65.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 61, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (53.67, 37.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 309, 1042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2776) [M::mem_pestat] mean and std.dev: (670.91, 698.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3643) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1184.907 CPU sec, 52.303 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 954737, 84, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 125, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (127.12, 67.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 64, 2045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6065) [M::mem_pestat] mean and std.dev: (780.71, 1474.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8075) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 445, 988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2564) [M::mem_pestat] mean and std.dev: (568.78, 541.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3352) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1181.837 CPU sec, 62.426 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (137, 953999, 66, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 127, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (124.87, 64.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 53, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 211) [M::mem_pestat] mean and std.dev: (50.15, 33.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 270) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 249, 797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2035) [M::mem_pestat] mean and std.dev: (489.61, 460.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2654) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1216.086 CPU sec, 74.882 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (173, 953440, 84, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 132, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (132.73, 73.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 48, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (50.85, 57.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 261, 900) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2322) [M::mem_pestat] mean and std.dev: (483.79, 489.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3033) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1192.616 CPU sec, 52.738 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (199, 954137, 86, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 124, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (118.67, 65.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.93) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 48, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 161) [M::mem_pestat] mean and std.dev: (42.35, 26.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 205) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 284, 1561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4269) [M::mem_pestat] mean and std.dev: (835.39, 1015.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5623) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1207.172 CPU sec, 71.547 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (172, 954099, 90, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 123, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (119.75, 71.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 56, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (54.10, 43.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 228, 809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2081) [M::mem_pestat] mean and std.dev: (447.77, 439.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2717) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1186.932 CPU sec, 52.181 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (167, 954048, 78, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 120, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (127.01, 70.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 57, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (69.34, 95.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 242, 765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1931) [M::mem_pestat] mean and std.dev: (396.18, 369.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2514) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1205.533 CPU sec, 71.638 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (164, 954309, 86, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 125, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (125.05, 60.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 42, 81) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 185) [M::mem_pestat] mean and std.dev: (39.49, 24.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 237) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (110, 195, 637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1691) [M::mem_pestat] mean and std.dev: (330.47, 368.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2218) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1198.166 CPU sec, 52.777 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (168, 954884, 84, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 124, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (121.90, 71.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 47, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (54.73, 51.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 210, 825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2173) [M::mem_pestat] mean and std.dev: (384.23, 411.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2847) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1163.243 CPU sec, 51.286 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (146, 954665, 86, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 133, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (126.50, 71.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.86, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 61, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (61.71, 60.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 234, 754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1916) [M::mem_pestat] mean and std.dev: (449.00, 451.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2497) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1212.472 CPU sec, 74.532 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (155, 954453, 72, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 120, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (129.69, 86.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 69, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1003) [M::mem_pestat] mean and std.dev: (71.21, 70.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1324) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 263, 992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2682) [M::mem_pestat] mean and std.dev: (494.32, 535.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1191.548 CPU sec, 52.515 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (173, 954303, 89, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 119, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (124.66, 74.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.92) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 52, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (54.40, 56.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 336, 911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2407) [M::mem_pestat] mean and std.dev: (518.07, 491.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3155) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1202.062 CPU sec, 71.846 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (164, 954591, 83, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 139, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (130.82, 68.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 31.93) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 69, 1903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5627) [M::mem_pestat] mean and std.dev: (895.37, 1585.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7489) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 230, 816) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2070) [M::mem_pestat] mean and std.dev: (468.95, 494.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2697) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1183.460 CPU sec, 52.085 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (181, 955090, 93, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 140, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (131.69, 67.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.93) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 50, 89) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 207) [M::mem_pestat] mean and std.dev: (47.36, 28.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 266) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 231, 886) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2314) [M::mem_pestat] mean and std.dev: (476.02, 480.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3028) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1213.401 CPU sec, 74.097 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (178, 954718, 84, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 126, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (121.20, 66.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 60, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (61.12, 62.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 433, 929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2423) [M::mem_pestat] mean and std.dev: (629.83, 584.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3170) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1195.750 CPU sec, 52.675 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (168, 954531, 77, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 134, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (130.69, 72.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 59, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037) [M::mem_pestat] mean and std.dev: (67.78, 94.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1371) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 266, 977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2605) [M::mem_pestat] mean and std.dev: (599.59, 642.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3419) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.086 CPU sec, 73.204 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (172, 954185, 76, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 135, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (132.80, 68.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.93) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 58, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2310) [M::mem_pestat] mean and std.dev: (178.18, 425.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3068) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 615, 1391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3793) [M::mem_pestat] mean and std.dev: (710.07, 697.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4994) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1203.811 CPU sec, 54.748 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (165, 954397, 90, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 110, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (113.91, 69.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 55, 744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2160) [M::mem_pestat] mean and std.dev: (167.84, 383.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2868) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 225, 767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1937) [M::mem_pestat] mean and std.dev: (416.17, 398.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2522) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1201.644 CPU sec, 71.609 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (173, 954245, 80, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 123, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (129.65, 71.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 46, 874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2566) [M::mem_pestat] mean and std.dev: (301.57, 609.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3412) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 250, 653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1581) [M::mem_pestat] mean and std.dev: (352.69, 313.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2045) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1193.227 CPU sec, 52.877 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (171, 953555, 89, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 118, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (117.10, 61.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 56, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (57.00, 61.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 258, 830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2210) [M::mem_pestat] mean and std.dev: (473.88, 444.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2900) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1217.999 CPU sec, 74.353 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (158, 954750, 78, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 132, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (127.55, 75.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.96) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 49, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1306) [M::mem_pestat] mean and std.dev: (69.45, 121.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1731) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 229, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 758) [M::mem_pestat] mean and std.dev: (212.58, 129.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1193.894 CPU sec, 52.650 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (159, 954205, 90, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 128, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (130.06, 67.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 49, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (51.61, 47.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 241, 867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2249) [M::mem_pestat] mean and std.dev: (537.35, 574.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2940) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1183.091 CPU sec, 54.582 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (156, 954691, 81, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 117, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (115.37, 65.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 58, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1574) [M::mem_pestat] mean and std.dev: (103.83, 225.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2087) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 264, 976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2532) [M::mem_pestat] mean and std.dev: (599.10, 685.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3342) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.637 CPU sec, 76.111 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (175, 954448, 88, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 114, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (118.91, 64.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 51, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (49.24, 42.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 325, 953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2503) [M::mem_pestat] mean and std.dev: (569.88, 530.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3278) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1200.021 CPU sec, 53.248 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 954499, 90, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 122, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (124.56, 73.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.73, 31.93) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 54, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 245) [M::mem_pestat] mean and std.dev: (49.62, 29.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 316) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (107, 202, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1502) [M::mem_pestat] mean and std.dev: (318.23, 328.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1967) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1201.742 CPU sec, 72.678 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (153, 953527, 87, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 131, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (129.78, 65.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 58, 1159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3407) [M::mem_pestat] mean and std.dev: (339.42, 751.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4531) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 614, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2437) [M::mem_pestat] mean and std.dev: (635.55, 522.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3173) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1187.833 CPU sec, 52.296 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (150, 954221, 75, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 121, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (129.33, 76.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 66, 753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2179) [M::mem_pestat] mean and std.dev: (174.21, 366.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2892) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 233, 681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1695) [M::mem_pestat] mean and std.dev: (447.54, 485.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2390) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1209.281 CPU sec, 73.537 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (146, 953777, 95, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 148, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (133.40, 83.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 47, 65) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 129) [M::mem_pestat] mean and std.dev: (43.40, 19.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 161) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 235, 679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1717) [M::mem_pestat] mean and std.dev: (329.48, 298.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2236) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1197.616 CPU sec, 52.773 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (170, 954199, 92, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 116, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (111.96, 60.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.87, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 51, 86) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 194) [M::mem_pestat] mean and std.dev: (44.99, 29.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 248) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 262, 705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1783) [M::mem_pestat] mean and std.dev: (369.56, 396.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2322) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.824 CPU sec, 73.800 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (168, 954293, 102, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 119, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (122.46, 71.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 63, 1100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3232) [M::mem_pestat] mean and std.dev: (284.64, 656.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4298) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (127, 263, 929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2533) [M::mem_pestat] mean and std.dev: (427.19, 408.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3335) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1195.651 CPU sec, 54.181 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (173, 954500, 88, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 127, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (126.10, 79.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 65, 1970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5836) [M::mem_pestat] mean and std.dev: (986.95, 1631.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7769) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 284, 804) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1948) [M::mem_pestat] mean and std.dev: (450.45, 396.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2520) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1183.436 CPU sec, 53.893 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (152, 954381, 68, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 133, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (132.11, 74.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 55, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1245) [M::mem_pestat] mean and std.dev: (79.13, 126.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1648) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 245, 828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2090) [M::mem_pestat] mean and std.dev: (428.52, 418.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2721) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1184.052 CPU sec, 52.679 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 954294, 78, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 118, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (123.58, 69.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 42, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (46.16, 42.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 277, 961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2473) [M::mem_pestat] mean and std.dev: (508.38, 527.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3229) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1188.937 CPU sec, 54.571 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (155, 953492, 80, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 136, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (128.07, 78.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 56, 1098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3236) [M::mem_pestat] mean and std.dev: (209.40, 558.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4305) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 309, 1525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4209) [M::mem_pestat] mean and std.dev: (876.31, 952.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5551) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1184.364 CPU sec, 52.166 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (154, 955505, 70, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 135, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (130.78, 68.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 51, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 133) [M::mem_pestat] mean and std.dev: (44.70, 17.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 165) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 211, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 784) [M::mem_pestat] mean and std.dev: (193.61, 128.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1184.946 CPU sec, 58.178 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (175, 954671, 76, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 114, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (118.96, 68.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.93) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 48, 65) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 135) [M::mem_pestat] mean and std.dev: (40.12, 18.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 170) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (127, 223, 1015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2791) [M::mem_pestat] mean and std.dev: (548.15, 592.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3679) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1223.535 CPU sec, 75.292 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 953799, 74, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 126, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (130.87, 65.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 63, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1097) [M::mem_pestat] mean and std.dev: (85.05, 121.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1451) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 215, 951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2595) [M::mem_pestat] mean and std.dev: (513.85, 572.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3417) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1192.327 CPU sec, 53.165 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (170, 954295, 91, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 129, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (125.11, 71.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 49, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (60.48, 91.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 960) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 666, 1112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2864) [M::mem_pestat] mean and std.dev: (669.52, 552.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3740) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1204.297 CPU sec, 72.010 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (154, 954874, 81, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 132, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (129.73, 72.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 50, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (59.38, 53.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2085) [M::mem_pestat] mean and std.dev: (391.29, 406.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2730) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1178.060 CPU sec, 51.846 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (153, 954572, 85, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 121, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (117.54, 64.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 54, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (64.91, 87.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 264, 783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2009) [M::mem_pestat] mean and std.dev: (391.33, 358.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2622) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1210.825 CPU sec, 72.572 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 954259, 92, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 132, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (127.95, 66.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 32.05) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 59, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (70.04, 95.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 268, 1248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3318) [M::mem_pestat] mean and std.dev: (716.08, 716.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4353) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1184.070 CPU sec, 52.150 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (161, 955299, 75, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 124, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (120.93, 66.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 61, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972) [M::mem_pestat] mean and std.dev: (81.63, 107.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1284) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 263, 824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2168) [M::mem_pestat] mean and std.dev: (347.70, 344.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2840) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1245.649 CPU sec, 73.963 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (160, 954263, 79, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 127, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (128.91, 75.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 51, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1516) [M::mem_pestat] mean and std.dev: (89.35, 141.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2010) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 245, 1184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3222) [M::mem_pestat] mean and std.dev: (552.51, 563.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4241) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1202.795 CPU sec, 54.837 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (144, 954689, 104, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 139, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (130.29, 76.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 46, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (45.56, 34.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 387) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 596, 1204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3260) [M::mem_pestat] mean and std.dev: (695.48, 714.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4288) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1210.099 CPU sec, 72.177 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (163, 954415, 69, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 127, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (124.19, 63.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.76, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 64, 1307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3845) [M::mem_pestat] mean and std.dev: (391.92, 798.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5114) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 279, 822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2120) [M::mem_pestat] mean and std.dev: (488.71, 431.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2769) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1189.226 CPU sec, 52.439 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 954338, 108, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 123, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (122.94, 68.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 47, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (57.56, 61.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 272, 939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2431) [M::mem_pestat] mean and std.dev: (481.16, 459.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3177) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1211.035 CPU sec, 72.167 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (161, 954797, 69, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 134, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (129.93, 70.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 59, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (59.29, 38.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 267, 753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1883) [M::mem_pestat] mean and std.dev: (474.29, 466.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2448) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1189.471 CPU sec, 52.704 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (170, 953732, 89, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 126, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (124.68, 68.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 56, 1199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3525) [M::mem_pestat] mean and std.dev: (270.51, 689.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4688) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 262, 1026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2668) [M::mem_pestat] mean and std.dev: (577.42, 622.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3489) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1200.125 CPU sec, 62.641 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (182, 954916, 97, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 127, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (124.87, 71.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.93) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 59, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (55.30, 43.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 227, 728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1840) [M::mem_pestat] mean and std.dev: (326.69, 295.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2396) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1240.536 CPU sec, 75.741 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (168, 953747, 92, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 130, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (126.17, 65.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 32.02) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 52, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (57.32, 56.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 320, 956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2434) [M::mem_pestat] mean and std.dev: (550.18, 437.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3173) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1197.957 CPU sec, 52.748 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (181, 954031, 81, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 132, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (125.68, 70.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 51, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1493) [M::mem_pestat] mean and std.dev: (106.02, 225.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1980) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 310, 1209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3187) [M::mem_pestat] mean and std.dev: (596.91, 588.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4176) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1212.615 CPU sec, 73.404 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (165, 954898, 86, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 126, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (122.33, 64.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.86, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 53, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (55.39, 42.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 252, 866) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2234) [M::mem_pestat] mean and std.dev: (490.54, 479.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2918) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1183.544 CPU sec, 52.118 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (165, 954315, 80, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 128, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (125.19, 69.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.78, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 54, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (48.74, 35.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 252, 901) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2423) [M::mem_pestat] mean and std.dev: (449.19, 396.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3184) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1213.574 CPU sec, 74.104 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (176, 954168, 90, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (101, 138, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (141.34, 70.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 63, 1365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4025) [M::mem_pestat] mean and std.dev: (552.52, 1019.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5355) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 298, 1091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2869) [M::mem_pestat] mean and std.dev: (517.23, 468.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3758) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1180.277 CPU sec, 51.926 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (161, 953918, 77, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 118, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (122.17, 64.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 54, 1254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3700) [M::mem_pestat] mean and std.dev: (311.09, 716.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4923) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 230, 1121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3049) [M::mem_pestat] mean and std.dev: (536.18, 615.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4013) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1241.621 CPU sec, 74.010 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (168, 954219, 74, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 138, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (124.53, 63.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 68, 1387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4091) [M::mem_pestat] mean and std.dev: (556.96, 987.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5443) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 223, 877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2321) [M::mem_pestat] mean and std.dev: (516.13, 539.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3043) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1207.457 CPU sec, 54.733 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (160, 954337, 90, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 137, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (128.71, 71.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.94) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 59, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (59.61, 47.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 209, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077) [M::mem_pestat] mean and std.dev: (200.94, 130.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1384) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1211.024 CPU sec, 72.684 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (155, 954235, 82, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 120, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (125.54, 76.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 51, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (58.17, 67.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 237, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2198) [M::mem_pestat] mean and std.dev: (497.47, 537.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2878) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1204.260 CPU sec, 53.016 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (163, 954397, 83, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 127, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (125.16, 68.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.73, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 62, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (57.88, 43.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (116, 188, 643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1697) [M::mem_pestat] mean and std.dev: (337.80, 418.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2224) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1209.093 CPU sec, 72.554 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (167, 954511, 73, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 122, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (120.38, 68.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.84, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 56, 784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2280) [M::mem_pestat] mean and std.dev: (160.85, 370.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3028) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 333, 1263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3435) [M::mem_pestat] mean and std.dev: (620.88, 712.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4521) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1198.095 CPU sec, 52.769 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (159, 955233, 111, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 117, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (121.53, 68.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 31.95) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 53, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 168) [M::mem_pestat] mean and std.dev: (46.48, 23.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 212) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 241, 888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2328) [M::mem_pestat] mean and std.dev: (464.82, 425.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3048) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1198.963 CPU sec, 65.612 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 954310, 93, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 121, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (128.55, 81.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.83, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 54, 823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2417) [M::mem_pestat] mean and std.dev: (160.05, 375.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3214) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 221, 1103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2999) [M::mem_pestat] mean and std.dev: (582.16, 611.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3947) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1217.798 CPU sec, 72.417 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (151, 954366, 80, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 123, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (122.09, 73.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.85, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 56, 1218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3578) [M::mem_pestat] mean and std.dev: (352.55, 767.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4758) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (123, 247, 1243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3483) [M::mem_pestat] mean and std.dev: (698.62, 789.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4603) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1200.118 CPU sec, 52.992 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (165, 955079, 90, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 127, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (126.54, 79.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.98) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 58, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011) [M::mem_pestat] mean and std.dev: (96.60, 156.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1336) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 243, 959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2513) [M::mem_pestat] mean and std.dev: (572.42, 543.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3290) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1201.116 CPU sec, 70.543 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (154, 953923, 87, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 122, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (123.88, 71.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 59, 1816) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5384) [M::mem_pestat] mean and std.dev: (852.11, 1606.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7279) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 274, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1410) [M::mem_pestat] mean and std.dev: (377.61, 274.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1806) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1191.982 CPU sec, 52.434 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (159, 954182, 94, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 130, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (129.60, 73.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 50, 97) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 229) [M::mem_pestat] mean and std.dev: (43.68, 24.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 295) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 259, 1044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2778) [M::mem_pestat] mean and std.dev: (530.03, 566.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3645) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1209.455 CPU sec, 73.126 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 954038, 85, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 137, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (121.86, 64.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.81, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 56, 1128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3310) [M::mem_pestat] mean and std.dev: (378.91, 792.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4401) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 268, 887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2361) [M::mem_pestat] mean and std.dev: (501.05, 499.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3098) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1188.134 CPU sec, 52.283 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 953519, 94, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 128, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (122.84, 63.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.80, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 58, 1210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3566) [M::mem_pestat] mean and std.dev: (424.15, 885.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4744) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 239, 653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1595) [M::mem_pestat] mean and std.dev: (382.91, 373.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2066) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1189.849 CPU sec, 53.106 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (139, 954517, 90, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 115, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 351) [M::mem_pestat] mean and std.dev: (114.68, 61.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.86, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 54, 102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 232) [M::mem_pestat] mean and std.dev: (52.61, 39.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 297) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 466, 1490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4092) [M::mem_pestat] mean and std.dev: (798.32, 936.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5393) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1255.430 CPU sec, 74.908 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (176, 954238, 81, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 127, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (126.96, 65.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.77, 31.93) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 55, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (58.73, 48.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 239, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2006) [M::mem_pestat] mean and std.dev: (411.69, 397.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2619) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1209.551 CPU sec, 54.889 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (143, 954380, 82, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 129, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (124.34, 75.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.82, 31.99) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 54, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (49.46, 37.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 263, 1075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2901) [M::mem_pestat] mean and std.dev: (540.47, 532.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3814) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1212.353 CPU sec, 72.736 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (163, 953903, 91, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 143, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (132.95, 75.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.75, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 57, 1181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3475) [M::mem_pestat] mean and std.dev: (436.04, 878.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4622) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 286, 1123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2963) [M::mem_pestat] mean and std.dev: (638.97, 619.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3883) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1198.594 CPU sec, 52.913 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 955061, 83, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 140, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (131.42, 68.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.74, 32.00) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 53, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (60.38, 69.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1056) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 255, 858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2186) [M::mem_pestat] mean and std.dev: (490.66, 464.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2850) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1207.879 CPU sec, 71.661 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (159, 953996, 89, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 127, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (123.46, 72.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.79, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 55, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 208) [M::mem_pestat] mean and std.dev: (49.85, 34.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 265) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 291, 1077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2837) [M::mem_pestat] mean and std.dev: (517.62, 554.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3717) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1189.535 CPU sec, 52.462 real sec [M::process] read 1637296 sequences (163729600 bp)... [M::process] 0 single-end sequences; 1637296 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (98, 650954, 70, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 136, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (136.77, 71.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 346, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (243, 448) [M::mem_pestat] mean and std.dev: (346.86, 31.97) [M::mem_pestat] low and high boundaries for proper pairs: (202, 489) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 63, 1089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3191) [M::mem_pestat] mean and std.dev: (310.02, 671.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4242) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 258, 650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1664) [M::mem_pestat] mean and std.dev: (420.27, 347.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2171) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1637296 reads in 817.754 CPU sec, 35.810 real sec [main] Version: 0.7.17-r1188 [main] CMD: /home/csyk2proj/tools/bwa.kit/bwa mem -p -t24 -R@RG\tID:KPGP-00252\tPL:ILLUMINA\tSM:KPGP-00252 -C /home/csyk2proj/data/bwa.kit/hs38DH.fa - [main] Real time: 56158.825 sec; CPU: 628050.756 sec