[M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (259, 944299, 88, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 102, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (116.66, 70.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 41, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (38.46, 31.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 240, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1019) [M::mem_pestat] mean and std.dev: (276.03, 192.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1298) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1186.075 CPU sec, 79.239 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (276, 944446, 92, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 119, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (123.10, 74.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 45, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (60.31, 107.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 238, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (203.65, 110.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 938) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1158.377 CPU sec, 53.227 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (246, 944018, 102, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 117, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (124.93, 76.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.84, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 39, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 163) [M::mem_pestat] mean and std.dev: (37.01, 25.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 210) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (117, 234, 599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1563) [M::mem_pestat] mean and std.dev: (249.64, 211.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2045) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1157.093 CPU sec, 72.664 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (233, 944031, 92, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 114, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (115.37, 66.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 57, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1289) [M::mem_pestat] mean and std.dev: (51.19, 56.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1708) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 232, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1165) [M::mem_pestat] mean and std.dev: (298.78, 238.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1492) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1147.705 CPU sec, 50.649 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (257, 944534, 103, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 110, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (124.69, 76.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 63, 1948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5776) [M::mem_pestat] mean and std.dev: (753.91, 1262.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7690) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 237, 790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2064) [M::mem_pestat] mean and std.dev: (328.83, 340.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2701) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1154.763 CPU sec, 71.272 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (256, 943469, 61, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 104, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (121.58, 80.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.67, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 51, 1093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3229) [M::mem_pestat] mean and std.dev: (279.28, 597.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4297) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 290, 1135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3089) [M::mem_pestat] mean and std.dev: (551.52, 599.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4066) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1145.155 CPU sec, 50.436 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (235, 944489, 92, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 116, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (118.24, 68.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 47, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (47.55, 38.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 245, 813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2069) [M::mem_pestat] mean and std.dev: (467.34, 478.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2697) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1158.944 CPU sec, 76.450 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (231, 943836, 105, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 108, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 377) [M::mem_pestat] mean and std.dev: (117.64, 69.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.20) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 46, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 153) [M::mem_pestat] mean and std.dev: (42.20, 24.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 194) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 247, 837) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2191) [M::mem_pestat] mean and std.dev: (377.64, 408.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2868) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1130.814 CPU sec, 51.490 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (256, 944668, 92, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 111, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (116.80, 70.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 48, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 227) [M::mem_pestat] mean and std.dev: (44.17, 37.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 295) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 181, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (171.57, 72.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1157.698 CPU sec, 72.441 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (256, 944252, 88, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 118, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (120.59, 69.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 48, 78) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 180) [M::mem_pestat] mean and std.dev: (41.51, 24.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 231) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 193, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (187.20, 96.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1142.701 CPU sec, 50.665 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (249, 943963, 85, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 122, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (129.17, 81.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 50, 74) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 172) [M::mem_pestat] mean and std.dev: (43.59, 29.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 221) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 207, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1439) [M::mem_pestat] mean and std.dev: (309.30, 335.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1875) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1157.662 CPU sec, 71.436 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (254, 943596, 79, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 115, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (123.33, 76.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 49, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (58.12, 53.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 421) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 219, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (197.31, 89.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1140.699 CPU sec, 50.497 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (260, 944421, 91, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 105, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (114.81, 66.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 41, 116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (49.59, 54.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 226, 893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2391) [M::mem_pestat] mean and std.dev: (417.97, 435.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3140) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1164.855 CPU sec, 76.787 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (232, 944090, 89, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 120, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (124.29, 75.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 50, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (53.99, 55.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 254, 921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2469) [M::mem_pestat] mean and std.dev: (498.60, 523.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3243) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1151.797 CPU sec, 53.227 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (246, 943415, 76, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 96, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (118.79, 81.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.31) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 60, 1428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4216) [M::mem_pestat] mean and std.dev: (451.84, 923.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5610) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 227, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (192.73, 83.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1155.810 CPU sec, 70.459 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (218, 944893, 72, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 112, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (115.42, 68.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.84, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 50, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 142) [M::mem_pestat] mean and std.dev: (40.19, 21.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 179) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (91, 171, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (172.62, 122.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1131.393 CPU sec, 50.134 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (249, 944378, 100, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 115, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (124.82, 78.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 48, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (62.96, 71.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (105, 214, 864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2382) [M::mem_pestat] mean and std.dev: (462.63, 505.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3141) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1147.995 CPU sec, 69.602 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (252, 945109, 85, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 117, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (118.88, 70.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 58, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 774) [M::mem_pestat] mean and std.dev: (74.49, 103.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 211, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 761) [M::mem_pestat] mean and std.dev: (251.30, 169.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1127.908 CPU sec, 50.041 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (268, 944382, 87, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 118, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (122.07, 72.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.68, 38.33) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 48, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992) [M::mem_pestat] mean and std.dev: (88.10, 150.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1313) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 211, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (178.04, 76.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1140.713 CPU sec, 57.157 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (254, 944567, 75, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 115, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (121.13, 77.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.86, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 54, 708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2072) [M::mem_pestat] mean and std.dev: (208.40, 450.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2754) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 249, 784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2054) [M::mem_pestat] mean and std.dev: (423.63, 467.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2689) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1163.648 CPU sec, 78.536 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (221, 944310, 85, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 114, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (116.84, 71.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 57, 1227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3607) [M::mem_pestat] mean and std.dev: (488.67, 907.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4797) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 293, 1108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2966) [M::mem_pestat] mean and std.dev: (612.94, 625.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3895) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1141.471 CPU sec, 50.484 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (250, 945555, 75, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 117, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (124.58, 74.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 55, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (52.05, 48.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 234, 785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2025) [M::mem_pestat] mean and std.dev: (414.05, 447.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2645) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1145.569 CPU sec, 68.031 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (231, 944692, 82, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 115, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (121.03, 70.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.19) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 44, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 261) [M::mem_pestat] mean and std.dev: (46.88, 49.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 220, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (185.78, 54.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1135.954 CPU sec, 50.290 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (255, 943877, 79, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 116, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (121.94, 70.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.32) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 56, 875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2569) [M::mem_pestat] mean and std.dev: (137.76, 294.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3416) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 241, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1449) [M::mem_pestat] mean and std.dev: (326.85, 310.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1882) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1147.514 CPU sec, 68.241 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (226, 944000, 80, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 117, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (120.78, 71.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 45, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 142) [M::mem_pestat] mean and std.dev: (43.03, 24.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 179) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 220, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (194.05, 97.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1127.061 CPU sec, 49.819 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (251, 944932, 89, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 117, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (123.67, 80.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.71, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 50, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (47.79, 41.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 267, 938) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2410) [M::mem_pestat] mean and std.dev: (550.87, 604.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3146) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1159.715 CPU sec, 76.114 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (229, 944723, 83, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 107, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (118.19, 69.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 52, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (57.53, 65.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 240, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (209.46, 107.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1130.397 CPU sec, 52.133 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (270, 943379, 83, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 119, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (123.61, 73.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.84, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 48, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (49.30, 55.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 228, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (210.97, 108.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1067) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1160.211 CPU sec, 70.210 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (244, 944464, 78, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 117, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (122.47, 72.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 41, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (40.49, 25.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 217, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 676) [M::mem_pestat] mean and std.dev: (198.71, 124.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1131.060 CPU sec, 50.086 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (238, 944424, 79, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 116, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (124.68, 73.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 48, 1723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5123) [M::mem_pestat] mean and std.dev: (866.77, 1507.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6898) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 245, 1016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2708) [M::mem_pestat] mean and std.dev: (446.64, 484.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3554) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1146.760 CPU sec, 68.775 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (232, 944274, 72, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 95, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (111.57, 76.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.33) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 48, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (51.25, 42.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 362) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 252, 718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1784) [M::mem_pestat] mean and std.dev: (319.94, 277.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1133.326 CPU sec, 50.099 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (258, 944577, 97, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 129, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (129.34, 77.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 57, 845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2473) [M::mem_pestat] mean and std.dev: (178.24, 413.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3287) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 225, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (218.55, 115.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1128.778 CPU sec, 51.033 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (231, 944434, 82, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 103, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (111.62, 69.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.85, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 49, 88) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 220) [M::mem_pestat] mean and std.dev: (43.35, 35.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 286) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 242, 795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2059) [M::mem_pestat] mean and std.dev: (321.66, 311.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2691) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1167.321 CPU sec, 76.955 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (241, 943691, 74, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 111, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (118.79, 71.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.71, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 43, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 262) [M::mem_pestat] mean and std.dev: (49.38, 50.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 224, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (226.74, 102.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1134.880 CPU sec, 52.254 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (226, 944443, 90, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 104, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (115.55, 75.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 48, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (56.81, 62.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 237, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029) [M::mem_pestat] mean and std.dev: (267.16, 215.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1312) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1154.281 CPU sec, 70.493 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (232, 945114, 83, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 91, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (103.42, 67.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 42, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (45.71, 38.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 398) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 216, 651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1667) [M::mem_pestat] mean and std.dev: (333.98, 349.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2175) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1131.360 CPU sec, 50.272 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (261, 944846, 88, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 117, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (126.04, 79.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 49, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (48.54, 41.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 292, 786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2014) [M::mem_pestat] mean and std.dev: (357.09, 292.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2628) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1147.251 CPU sec, 70.354 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (273, 944605, 80, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 102, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (114.64, 74.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.20) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 43, 77) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 179) [M::mem_pestat] mean and std.dev: (40.80, 25.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 230) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 243, 1123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3039) [M::mem_pestat] mean and std.dev: (525.77, 562.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3997) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1133.632 CPU sec, 49.995 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (233, 944687, 79, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 121, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (126.49, 71.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 53, 743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2159) [M::mem_pestat] mean and std.dev: (165.32, 388.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2867) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 177, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 562) [M::mem_pestat] mean and std.dev: (172.81, 58.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1156.663 CPU sec, 76.874 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (234, 944991, 81, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 112, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (117.41, 69.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 53, 112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (59.57, 59.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 358) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 241, 784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1978) [M::mem_pestat] mean and std.dev: (390.63, 394.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2575) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1139.433 CPU sec, 52.667 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (256, 944126, 90, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 120, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (124.09, 78.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 49, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (51.99, 54.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 426) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 229, 552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1294) [M::mem_pestat] mean and std.dev: (296.71, 275.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1665) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1145.466 CPU sec, 70.498 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (249, 944974, 79, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 111, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (116.59, 69.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.70, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 69, 2112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6268) [M::mem_pestat] mean and std.dev: (924.84, 1397.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8346) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 221, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 709) [M::mem_pestat] mean and std.dev: (184.94, 104.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1138.544 CPU sec, 50.506 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (239, 944058, 91, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 117, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (120.38, 71.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.71, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 42, 77) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 183) [M::mem_pestat] mean and std.dev: (39.20, 29.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 236) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (114, 211, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 687) [M::mem_pestat] mean and std.dev: (183.73, 102.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1151.336 CPU sec, 70.654 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (221, 944214, 80, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 126, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (128.62, 76.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 49, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 180) [M::mem_pestat] mean and std.dev: (41.76, 20.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 230) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 225, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 987) [M::mem_pestat] mean and std.dev: (250.66, 234.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1269) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1129.598 CPU sec, 50.009 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (213, 944798, 77, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 109, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (115.46, 70.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 49, 1335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3955) [M::mem_pestat] mean and std.dev: (448.07, 924.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5265) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 213, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2058) [M::mem_pestat] mean and std.dev: (466.54, 520.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2690) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1159.025 CPU sec, 76.697 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (244, 944412, 88, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 131, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (130.36, 76.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.86, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 53, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (65.94, 89.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 265, 938) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2478) [M::mem_pestat] mean and std.dev: (409.46, 441.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3248) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1130.689 CPU sec, 51.918 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (218, 944356, 90, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 120, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (126.65, 74.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 62, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2310) [M::mem_pestat] mean and std.dev: (143.17, 303.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3068) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 245, 863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2317) [M::mem_pestat] mean and std.dev: (462.11, 504.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3044) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1152.401 CPU sec, 70.750 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (220, 944192, 96, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 118, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (121.86, 74.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 48, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (51.32, 41.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 253, 685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1681) [M::mem_pestat] mean and std.dev: (372.83, 341.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2179) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1127.580 CPU sec, 50.019 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (253, 944056, 94, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 106, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (111.59, 69.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 53, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 973) [M::mem_pestat] mean and std.dev: (66.15, 88.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1286) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (132, 181, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (159.71, 62.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1146.772 CPU sec, 70.668 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (239, 944790, 91, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 108, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (113.78, 68.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 49, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (50.04, 47.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 239, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1244) [M::mem_pestat] mean and std.dev: (346.33, 286.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1606) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1128.661 CPU sec, 49.840 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (229, 945110, 90, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 118, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (122.49, 75.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 47, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (74.38, 110.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 925) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 239, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957) [M::mem_pestat] mean and std.dev: (268.07, 180.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1214) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1140.891 CPU sec, 61.627 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (234, 945218, 87, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 113, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (122.14, 76.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.86, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 53, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2147) [M::mem_pestat] mean and std.dev: (179.61, 431.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2854) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 563, 1552) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4240) [M::mem_pestat] mean and std.dev: (873.11, 820.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5584) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1154.446 CPU sec, 77.906 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (237, 944965, 82, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 115, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (120.86, 72.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.71, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 42, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (59.52, 96.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 223, 628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1522) [M::mem_pestat] mean and std.dev: (348.77, 289.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1969) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1131.199 CPU sec, 50.276 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (222, 945001, 60, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 125, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (127.64, 75.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 47, 1839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5463) [M::mem_pestat] mean and std.dev: (783.52, 1487.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7275) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 283, 684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1724) [M::mem_pestat] mean and std.dev: (454.21, 450.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2256) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1145.901 CPU sec, 67.272 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (259, 944076, 95, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 111, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (119.11, 70.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 47, 74) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 168) [M::mem_pestat] mean and std.dev: (39.39, 25.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 215) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 250, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (201.53, 92.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1129.207 CPU sec, 49.786 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (259, 944836, 84, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 117, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (124.65, 77.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.21) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 41, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (43.96, 42.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 264, 1145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2967) [M::mem_pestat] mean and std.dev: (583.73, 603.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3878) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1145.931 CPU sec, 70.357 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (254, 943315, 76, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 111, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (125.27, 78.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 52, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (54.19, 46.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 894) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 229, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (210.19, 58.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1128.915 CPU sec, 49.889 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (265, 944051, 93, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 116, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (120.93, 71.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.70, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 48, 746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2172) [M::mem_pestat] mean and std.dev: (196.09, 448.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2885) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 252, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1445) [M::mem_pestat] mean and std.dev: (273.39, 267.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1876) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1158.643 CPU sec, 76.903 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (268, 944466, 77, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 110, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (122.77, 77.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.21) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 49, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (56.23, 83.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (106, 213, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (180.02, 101.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1130.511 CPU sec, 52.084 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (245, 945033, 92, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 108, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (119.23, 73.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.69, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 46, 703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2059) [M::mem_pestat] mean and std.dev: (107.81, 330.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2737) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 250, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2605) [M::mem_pestat] mean and std.dev: (434.41, 532.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3425) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1139.573 CPU sec, 66.255 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (269, 945629, 89, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 120, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (125.67, 75.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 45, 95) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 227) [M::mem_pestat] mean and std.dev: (47.14, 37.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 293) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 222, 777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1983) [M::mem_pestat] mean and std.dev: (324.89, 331.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2586) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1127.827 CPU sec, 50.167 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (227, 945537, 94, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 94, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (108.26, 66.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.68, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 40, 79) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 183) [M::mem_pestat] mean and std.dev: (34.94, 21.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 235) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 232, 893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2413) [M::mem_pestat] mean and std.dev: (491.12, 547.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3173) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1147.787 CPU sec, 70.811 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (263, 944713, 100, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 125, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (126.17, 74.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 52, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1662) [M::mem_pestat] mean and std.dev: (91.53, 193.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2207) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 242, 914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2358) [M::mem_pestat] mean and std.dev: (510.81, 552.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3080) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1133.507 CPU sec, 50.067 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (230, 944648, 101, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 116, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (122.05, 73.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 40, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (42.52, 41.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 344) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 316, 1024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2648) [M::mem_pestat] mean and std.dev: (645.27, 600.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3460) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1125.255 CPU sec, 55.377 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (278, 944903, 78, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 106, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (114.65, 70.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 61, 2276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6776) [M::mem_pestat] mean and std.dev: (986.73, 1648.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9026) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 245, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1371) [M::mem_pestat] mean and std.dev: (337.65, 311.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1158.576 CPU sec, 76.028 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (269, 944076, 78, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 117, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (123.70, 74.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.31) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 46, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 160) [M::mem_pestat] mean and std.dev: (39.21, 20.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 204) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (134, 186, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (205.64, 117.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1134.294 CPU sec, 52.610 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (224, 944100, 94, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 131, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (124.72, 75.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 50, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (49.57, 44.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (122, 250, 699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1853) [M::mem_pestat] mean and std.dev: (348.53, 349.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2430) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1148.998 CPU sec, 70.247 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (238, 943812, 89, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 106, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (120.01, 74.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 53, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1537) [M::mem_pestat] mean and std.dev: (96.73, 206.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2040) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 272, 685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1621) [M::mem_pestat] mean and std.dev: (351.62, 287.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2089) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1128.935 CPU sec, 50.077 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (269, 943237, 71, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 111, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (119.25, 74.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.83, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 43, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 161) [M::mem_pestat] mean and std.dev: (39.64, 23.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 206) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 224, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (220.48, 65.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1146.649 CPU sec, 69.617 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (267, 944109, 89, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 117, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (119.77, 74.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 56, 1199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3537) [M::mem_pestat] mean and std.dev: (466.10, 947.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4706) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 225, 568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1328) [M::mem_pestat] mean and std.dev: (344.70, 265.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1708) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1129.789 CPU sec, 49.872 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (254, 945584, 106, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 116, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (121.91, 69.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 46, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 153) [M::mem_pestat] mean and std.dev: (42.39, 22.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 194) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (127, 209, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (191.90, 106.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1157.704 CPU sec, 76.269 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (253, 943217, 89, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 122, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (120.06, 68.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 38, 65) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 147) [M::mem_pestat] mean and std.dev: (33.61, 20.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 188) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 234, 824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2184) [M::mem_pestat] mean and std.dev: (400.57, 373.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2864) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1136.201 CPU sec, 52.555 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (288, 944579, 77, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 111, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (119.04, 75.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 37, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 174) [M::mem_pestat] mean and std.dev: (34.10, 23.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 225) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 241, 522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1240) [M::mem_pestat] mean and std.dev: (294.68, 236.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1599) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1149.643 CPU sec, 70.049 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (244, 944512, 86, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 121, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (121.76, 76.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 44, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (54.37, 62.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (121, 228, 606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1576) [M::mem_pestat] mean and std.dev: (339.86, 368.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2061) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1132.842 CPU sec, 50.490 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (249, 944133, 84, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 125, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (126.29, 76.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 49, 1524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4514) [M::mem_pestat] mean and std.dev: (765.58, 1372.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6256) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 215, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (203.43, 105.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1146.803 CPU sec, 68.826 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (252, 944654, 79, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 111, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (119.59, 72.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 37, 70) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 166) [M::mem_pestat] mean and std.dev: (38.97, 24.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 214) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 219, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (187.91, 102.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 708) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1135.236 CPU sec, 50.082 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (266, 944327, 78, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 117, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (120.17, 73.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 54, 109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (51.84, 45.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 349) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 278, 1085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2953) [M::mem_pestat] mean and std.dev: (598.89, 649.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3887) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1116.953 CPU sec, 49.687 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (267, 944256, 80, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 102, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (113.64, 73.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 43, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (48.79, 51.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 309, 1161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3087) [M::mem_pestat] mean and std.dev: (541.79, 621.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4050) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1147.844 CPU sec, 76.407 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (265, 944319, 89, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 113, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (122.00, 72.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.84, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 46, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (52.90, 82.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1244) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 228, 588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1434) [M::mem_pestat] mean and std.dev: (296.48, 249.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1857) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1130.795 CPU sec, 52.314 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (264, 943919, 79, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 112, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (122.29, 71.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 45, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 162) [M::mem_pestat] mean and std.dev: (39.00, 20.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 207) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 251, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1330) [M::mem_pestat] mean and std.dev: (342.64, 283.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1710) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1141.834 CPU sec, 68.348 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (229, 944192, 79, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 110, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (123.33, 77.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 43, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (50.11, 44.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 222, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (200.81, 134.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 969) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1127.912 CPU sec, 50.003 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (264, 944562, 95, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 121, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (123.93, 74.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 56, 1519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4483) [M::mem_pestat] mean and std.dev: (800.60, 1487.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6749) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 326, 849) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2225) [M::mem_pestat] mean and std.dev: (508.29, 487.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2913) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1144.684 CPU sec, 67.948 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (242, 943368, 87, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 116, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (121.91, 68.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 48, 77) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 169) [M::mem_pestat] mean and std.dev: (44.89, 28.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 215) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 253, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (231.36, 59.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1128.400 CPU sec, 49.937 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (242, 944923, 92, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 116, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (119.07, 75.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 49, 110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 262) [M::mem_pestat] mean and std.dev: (54.42, 42.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 235, 576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1334) [M::mem_pestat] mean and std.dev: (300.52, 223.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1713) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1158.210 CPU sec, 76.296 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (259, 944150, 63, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 105, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (111.05, 67.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.83, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 54, 844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2484) [M::mem_pestat] mean and std.dev: (234.47, 505.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3304) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 234, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (193.16, 80.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1136.770 CPU sec, 52.321 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (254, 944621, 71, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 106, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (124.10, 79.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.31) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 63, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1096) [M::mem_pestat] mean and std.dev: (81.36, 153.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1451) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 243, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (238.06, 139.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1104) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1147.511 CPU sec, 68.614 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (241, 944486, 76, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 119, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (123.32, 78.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 48, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 310) [M::mem_pestat] mean and std.dev: (53.59, 49.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 281, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1898) [M::mem_pestat] mean and std.dev: (397.62, 314.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2457) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1131.421 CPU sec, 50.311 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (253, 943595, 89, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 129, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (129.77, 78.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.34) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 45, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 238) [M::mem_pestat] mean and std.dev: (51.73, 46.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 308) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 273, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1049) [M::mem_pestat] mean and std.dev: (271.71, 168.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1339) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1147.213 CPU sec, 69.633 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (240, 945071, 94, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 121, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (123.42, 76.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.31) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 41, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (47.20, 43.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 481) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 269, 1131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3013) [M::mem_pestat] mean and std.dev: (569.93, 619.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3954) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1131.505 CPU sec, 50.083 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (242, 943844, 74, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 138, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (133.07, 79.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 42, 70) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 166) [M::mem_pestat] mean and std.dev: (35.85, 24.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 214) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 218, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (187.19, 70.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1164.761 CPU sec, 76.882 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (253, 943976, 70, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 118, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (121.90, 78.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.68, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 53, 690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2024) [M::mem_pestat] mean and std.dev: (220.51, 488.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2691) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 237, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1153) [M::mem_pestat] mean and std.dev: (282.33, 242.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1485) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1125.295 CPU sec, 52.006 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (276, 944782, 86, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 116, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (123.53, 73.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.20) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 55, 112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 268) [M::mem_pestat] mean and std.dev: (54.76, 40.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (117, 212, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (172.52, 85.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 905) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1137.570 CPU sec, 64.507 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (266, 944391, 89, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 103, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (120.47, 78.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 47, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (49.21, 45.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 263, 653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1663) [M::mem_pestat] mean and std.dev: (370.98, 338.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2168) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1126.937 CPU sec, 49.981 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (242, 945013, 66, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 97, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (108.37, 65.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 42, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (47.85, 50.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 221, 845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2221) [M::mem_pestat] mean and std.dev: (338.68, 336.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2909) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1140.114 CPU sec, 68.865 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (252, 943851, 101, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 126, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (125.40, 80.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 61, 1339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3961) [M::mem_pestat] mean and std.dev: (461.06, 917.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5272) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (122, 205, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (190.88, 113.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 678) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1127.444 CPU sec, 49.877 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (218, 944257, 90, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 93, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (111.38, 72.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.32) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 52, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (51.61, 40.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 441) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 236, 903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2407) [M::mem_pestat] mean and std.dev: (360.00, 424.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3159) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1134.126 CPU sec, 60.460 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (243, 944682, 84, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 112, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (124.64, 76.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 47, 415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1179) [M::mem_pestat] mean and std.dev: (74.80, 116.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1561) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 231, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 939) [M::mem_pestat] mean and std.dev: (239.76, 170.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1206) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1156.895 CPU sec, 77.304 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (259, 945030, 87, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 106, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (118.00, 77.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.32) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 43, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 149) [M::mem_pestat] mean and std.dev: (40.61, 22.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 189) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 228, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (193.48, 79.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1132.872 CPU sec, 50.496 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (242, 943939, 101, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 106, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (116.69, 69.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.69, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 45, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (63.06, 72.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 256, 847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2215) [M::mem_pestat] mean and std.dev: (434.69, 458.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2899) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1142.194 CPU sec, 68.543 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (221, 943935, 103, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 112, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (120.61, 74.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 43, 66) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 156) [M::mem_pestat] mean and std.dev: (40.21, 23.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 201) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 222, 685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1765) [M::mem_pestat] mean and std.dev: (293.36, 270.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2305) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1127.380 CPU sec, 50.063 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (244, 943990, 93, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 104, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (114.42, 72.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 52, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 250) [M::mem_pestat] mean and std.dev: (52.72, 42.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 323) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 234, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (215.52, 126.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1149.089 CPU sec, 68.205 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (255, 944363, 78, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 113, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (116.57, 66.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 46, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (61.45, 79.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 263, 1139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3023) [M::mem_pestat] mean and std.dev: (579.43, 663.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3965) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1132.776 CPU sec, 50.078 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (228, 943601, 87, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 104, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (112.15, 73.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 51, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (63.42, 77.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 219, 611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1575) [M::mem_pestat] mean and std.dev: (256.68, 249.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2057) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1161.433 CPU sec, 75.951 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (238, 944191, 115, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 121, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (121.25, 74.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 49, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (46.37, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 257, 910) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2334) [M::mem_pestat] mean and std.dev: (442.56, 423.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3046) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1137.587 CPU sec, 52.557 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (281, 944108, 78, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 120, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (123.25, 69.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 62, 1610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4768) [M::mem_pestat] mean and std.dev: (707.99, 1305.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6347) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 245, 1006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2676) [M::mem_pestat] mean and std.dev: (448.62, 474.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3511) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1157.453 CPU sec, 71.110 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (250, 944224, 96, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 111, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (115.29, 69.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 48, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 267) [M::mem_pestat] mean and std.dev: (42.42, 28.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 253, 1082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2920) [M::mem_pestat] mean and std.dev: (522.46, 578.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3839) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1136.138 CPU sec, 50.669 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (228, 944289, 90, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 122, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (127.71, 80.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.21) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 39, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 192) [M::mem_pestat] mean and std.dev: (37.22, 28.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 250) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 250, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2369) [M::mem_pestat] mean and std.dev: (449.61, 504.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3113) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1145.999 CPU sec, 69.122 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (257, 944905, 88, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 119, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (117.46, 71.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 51, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (50.92, 38.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 266, 906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2344) [M::mem_pestat] mean and std.dev: (533.87, 546.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3063) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1122.993 CPU sec, 49.741 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (263, 944983, 95, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 121, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (122.45, 77.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.21) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 44, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (71.86, 124.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 271, 976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2534) [M::mem_pestat] mean and std.dev: (463.67, 447.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3313) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1125.350 CPU sec, 54.499 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (238, 944279, 81, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 122, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (121.03, 71.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.85, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 48, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (49.55, 43.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 221, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1265) [M::mem_pestat] mean and std.dev: (254.84, 235.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1643) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1155.689 CPU sec, 75.949 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (261, 944758, 78, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 104, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (116.79, 72.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 53, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (50.95, 50.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 887) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 250, 1068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2884) [M::mem_pestat] mean and std.dev: (520.52, 552.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3792) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1139.270 CPU sec, 52.598 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (268, 944630, 79, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 115, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (122.15, 70.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.33) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 46, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (62.03, 61.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 238, 694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1780) [M::mem_pestat] mean and std.dev: (352.70, 331.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2323) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1139.322 CPU sec, 68.001 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (229, 943882, 70, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 101, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (114.33, 67.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 45, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259) [M::mem_pestat] mean and std.dev: (44.25, 39.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 249, 753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1885) [M::mem_pestat] mean and std.dev: (452.14, 429.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2451) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1133.367 CPU sec, 50.475 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (253, 944568, 75, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 126, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (125.67, 71.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 54, 690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2004) [M::mem_pestat] mean and std.dev: (122.07, 309.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2661) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 232, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (241.97, 151.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1107) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1153.310 CPU sec, 70.610 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (253, 944034, 81, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 111, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (119.76, 72.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 43, 67) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 149) [M::mem_pestat] mean and std.dev: (37.77, 20.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 190) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 226, 763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1939) [M::mem_pestat] mean and std.dev: (455.10, 471.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1124.702 CPU sec, 49.610 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (228, 944356, 98, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 98, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (111.84, 69.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 48, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (48.81, 50.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 472) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 240, 1279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3537) [M::mem_pestat] mean and std.dev: (527.45, 639.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4666) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1152.590 CPU sec, 76.157 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (255, 944219, 85, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 132, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (133.95, 75.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 49, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (54.91, 51.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 280, 932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2390) [M::mem_pestat] mean and std.dev: (529.97, 458.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3119) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1131.733 CPU sec, 52.060 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (247, 944603, 91, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 108, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (121.26, 74.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 54, 709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2085) [M::mem_pestat] mean and std.dev: (128.12, 327.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2773) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (123, 233, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 708) [M::mem_pestat] mean and std.dev: (203.74, 123.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1148.132 CPU sec, 71.261 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (259, 944907, 87, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 108, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (116.59, 73.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 51, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 680) [M::mem_pestat] mean and std.dev: (74.63, 98.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 299, 847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2201) [M::mem_pestat] mean and std.dev: (435.53, 428.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2878) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1128.215 CPU sec, 50.212 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (271, 944289, 77, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 119, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (121.04, 71.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 46, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (44.60, 48.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 215, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (192.85, 90.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1148.825 CPU sec, 70.920 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (261, 944815, 92, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 113, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (120.87, 74.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 60, 777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2267) [M::mem_pestat] mean and std.dev: (222.53, 484.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3012) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 239, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1968) [M::mem_pestat] mean and std.dev: (456.34, 508.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2569) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1129.449 CPU sec, 50.038 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (248, 944192, 82, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 97, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (111.83, 73.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 62, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (68.54, 71.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 248, 836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2190) [M::mem_pestat] mean and std.dev: (470.07, 510.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2867) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1120.365 CPU sec, 49.452 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (237, 944123, 85, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 135, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (138.47, 81.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 42, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (49.38, 54.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 241, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1161) [M::mem_pestat] mean and std.dev: (293.81, 258.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1497) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1167.882 CPU sec, 77.252 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (233, 943833, 85, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 117, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (122.25, 71.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 38, 86) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 212) [M::mem_pestat] mean and std.dev: (38.27, 27.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 275) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (124, 194, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1345) [M::mem_pestat] mean and std.dev: (285.38, 288.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1752) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1140.282 CPU sec, 52.720 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (253, 944029, 85, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 118, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (128.86, 80.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 39, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 715) [M::mem_pestat] mean and std.dev: (40.69, 50.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (118, 210, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (205.97, 154.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1146.497 CPU sec, 70.653 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (215, 943839, 98, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 103, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (115.47, 69.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 47, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259) [M::mem_pestat] mean and std.dev: (48.15, 38.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 264, 832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2126) [M::mem_pestat] mean and std.dev: (433.32, 396.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1126.123 CPU sec, 50.011 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (225, 944437, 83, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 118, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (125.59, 72.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.84, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 54, 822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2402) [M::mem_pestat] mean and std.dev: (85.31, 185.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3192) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 263, 706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1774) [M::mem_pestat] mean and std.dev: (346.74, 317.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2308) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1142.674 CPU sec, 69.800 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (243, 944327, 80, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 115, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (121.09, 76.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 54, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 980) [M::mem_pestat] mean and std.dev: (63.26, 105.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1298) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 260, 787) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2011) [M::mem_pestat] mean and std.dev: (352.62, 392.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2623) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1126.031 CPU sec, 49.936 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (254, 944715, 96, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 106, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (121.31, 76.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 47, 75) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 165) [M::mem_pestat] mean and std.dev: (39.81, 19.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 210) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 289, 1254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3430) [M::mem_pestat] mean and std.dev: (585.55, 641.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4518) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1157.453 CPU sec, 76.583 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (251, 943692, 89, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 108, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (117.36, 63.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 40, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 160) [M::mem_pestat] mean and std.dev: (37.30, 23.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 206) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 265, 865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2273) [M::mem_pestat] mean and std.dev: (451.27, 451.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2977) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1129.104 CPU sec, 52.232 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (274, 944713, 97, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 113, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (118.85, 70.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.87, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 49, 89) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 227) [M::mem_pestat] mean and std.dev: (47.11, 38.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 296) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 240, 669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1745) [M::mem_pestat] mean and std.dev: (286.65, 274.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2283) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1145.410 CPU sec, 69.619 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (246, 944320, 105, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 111, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (125.15, 79.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 42, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 173) [M::mem_pestat] mean and std.dev: (38.74, 25.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 223) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 221, 873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2289) [M::mem_pestat] mean and std.dev: (412.42, 443.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2997) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1124.200 CPU sec, 50.026 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (255, 945142, 76, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 117, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (122.69, 70.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 54, 102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 238) [M::mem_pestat] mean and std.dev: (49.87, 33.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 306) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 247, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1478) [M::mem_pestat] mean and std.dev: (337.66, 327.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1922) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1147.167 CPU sec, 70.485 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (237, 945202, 72, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 112, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (122.07, 77.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 44, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 196) [M::mem_pestat] mean and std.dev: (34.93, 21.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 254) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 229, 714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1798) [M::mem_pestat] mean and std.dev: (416.39, 436.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2340) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1130.167 CPU sec, 50.093 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (278, 944478, 103, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 113, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (120.25, 74.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 37, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 263) [M::mem_pestat] mean and std.dev: (40.42, 37.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 343) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (126, 222, 622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1614) [M::mem_pestat] mean and std.dev: (313.68, 293.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2110) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1155.706 CPU sec, 76.723 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (211, 944442, 85, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 106, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (114.21, 72.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 45, 769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2245) [M::mem_pestat] mean and std.dev: (159.03, 395.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2983) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 237, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1176) [M::mem_pestat] mean and std.dev: (264.13, 215.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1514) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1132.296 CPU sec, 52.117 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (266, 944646, 84, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 101, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (113.28, 73.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 61, 1081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3187) [M::mem_pestat] mean and std.dev: (352.87, 759.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4240) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 270, 786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2004) [M::mem_pestat] mean and std.dev: (428.07, 456.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2613) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1146.733 CPU sec, 70.236 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (252, 944097, 90, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 128, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (137.51, 81.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.31) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 36, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 175) [M::mem_pestat] mean and std.dev: (36.62, 32.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 227) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 235, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (213.89, 95.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1121.144 CPU sec, 49.947 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (242, 945401, 91, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 116, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (118.54, 72.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 45, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (45.29, 47.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 423) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (128, 243, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1583) [M::mem_pestat] mean and std.dev: (371.31, 367.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2068) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1127.742 CPU sec, 64.318 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (250, 944854, 87, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 122, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (123.43, 71.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 44, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 160) [M::mem_pestat] mean and std.dev: (41.23, 25.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 204) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 264, 716) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1818) [M::mem_pestat] mean and std.dev: (363.19, 319.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2369) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1126.753 CPU sec, 49.719 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (246, 944781, 85, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 121, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (124.85, 72.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 40, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 159) [M::mem_pestat] mean and std.dev: (36.51, 22.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 204) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 261, 1086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2888) [M::mem_pestat] mean and std.dev: (589.37, 644.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3789) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1132.617 CPU sec, 59.383 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (247, 944249, 94, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 114, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (119.60, 71.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 49, 79) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 183) [M::mem_pestat] mean and std.dev: (40.79, 23.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 235) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (117, 190, 696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1854) [M::mem_pestat] mean and std.dev: (375.57, 393.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2433) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1149.522 CPU sec, 74.993 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (254, 943860, 85, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 104, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (115.38, 73.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.32) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 48, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (54.01, 50.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 253, 810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2090) [M::mem_pestat] mean and std.dev: (364.05, 380.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2730) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1135.129 CPU sec, 50.250 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (232, 943778, 79, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 105, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (115.41, 68.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 55, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (61.56, 66.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 215, 557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1335) [M::mem_pestat] mean and std.dev: (284.10, 215.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1724) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1140.791 CPU sec, 66.886 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (228, 944154, 64, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 110, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (120.04, 73.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.86, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 54, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (47.52, 32.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 237, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 857) [M::mem_pestat] mean and std.dev: (244.70, 129.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1077) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1122.798 CPU sec, 49.513 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (252, 943622, 85, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 123, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (125.75, 74.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 46, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (48.97, 52.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 220, 852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2236) [M::mem_pestat] mean and std.dev: (348.07, 328.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2928) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1142.094 CPU sec, 68.386 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (217, 945164, 96, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 119, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (124.98, 77.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 48, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1550) [M::mem_pestat] mean and std.dev: (90.36, 175.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2057) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 249, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (220.06, 103.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 809) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1119.064 CPU sec, 49.384 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (195, 944741, 79, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 119, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (120.06, 71.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 50, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (64.48, 79.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 259, 722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1762) [M::mem_pestat] mean and std.dev: (451.25, 429.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2282) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1158.382 CPU sec, 76.274 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (256, 944343, 82, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 120, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (119.63, 72.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 54, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (71.18, 86.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 249, 871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2303) [M::mem_pestat] mean and std.dev: (453.97, 499.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3019) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1132.990 CPU sec, 52.477 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (241, 944471, 74, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 129, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (127.29, 68.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 50, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (48.81, 43.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 751) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 256, 1133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3021) [M::mem_pestat] mean and std.dev: (558.36, 653.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3965) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1138.198 CPU sec, 68.025 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (239, 944441, 85, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 133, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (134.79, 80.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 46, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (53.93, 72.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 200, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1226) [M::mem_pestat] mean and std.dev: (300.59, 263.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1580) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1126.358 CPU sec, 50.117 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (215, 944566, 93, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 112, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (124.58, 76.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 42, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (48.78, 48.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 738) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 264, 702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1684) [M::mem_pestat] mean and std.dev: (438.43, 349.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2175) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1145.164 CPU sec, 70.051 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (236, 944539, 95, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 106, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (113.92, 70.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 49, 704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2052) [M::mem_pestat] mean and std.dev: (189.74, 392.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2726) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 218, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 920) [M::mem_pestat] mean and std.dev: (239.83, 198.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1132.083 CPU sec, 50.007 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (234, 944189, 95, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 114, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (118.39, 71.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 52, 982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2878) [M::mem_pestat] mean and std.dev: (274.10, 626.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3826) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 211, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (199.17, 116.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1132.471 CPU sec, 54.450 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (221, 943989, 93, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 109, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (119.78, 77.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 45, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 216) [M::mem_pestat] mean and std.dev: (45.32, 33.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 279) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 250, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1454) [M::mem_pestat] mean and std.dev: (327.42, 253.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1872) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1166.060 CPU sec, 77.483 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (221, 944762, 88, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 127, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (123.55, 66.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.70, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 51, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 983) [M::mem_pestat] mean and std.dev: (74.74, 128.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1299) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 225, 558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1344) [M::mem_pestat] mean and std.dev: (266.13, 250.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1737) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1137.975 CPU sec, 52.753 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (228, 944618, 73, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 110, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (115.78, 71.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.31) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 53, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (64.40, 54.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 266, 714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1788) [M::mem_pestat] mean and std.dev: (418.12, 390.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2325) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1136.647 CPU sec, 67.325 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (237, 943518, 88, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 117, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (125.65, 76.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 52, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (52.94, 45.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 655) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 221, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (235.50, 175.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1101) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1125.868 CPU sec, 49.838 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (242, 944201, 85, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 108, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (121.24, 76.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.71, 38.34) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 48, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (64.14, 67.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 251, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (210.78, 84.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1143.686 CPU sec, 68.122 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (244, 944323, 72, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 106, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (116.36, 72.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 51, 758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2202) [M::mem_pestat] mean and std.dev: (182.53, 426.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2924) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 232, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (232.17, 142.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1123.076 CPU sec, 49.592 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (258, 944625, 91, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 120, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (119.04, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.34) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 50, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (59.60, 77.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 263, 1102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3006) [M::mem_pestat] mean and std.dev: (617.48, 701.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3958) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1161.895 CPU sec, 77.199 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (257, 943762, 77, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 113, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (122.92, 76.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.33) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 46, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 253) [M::mem_pestat] mean and std.dev: (47.30, 33.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (130, 256, 568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1444) [M::mem_pestat] mean and std.dev: (311.10, 296.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1882) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1134.632 CPU sec, 52.233 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (274, 944768, 89, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 110, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (118.77, 69.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.31) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 46, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (42.96, 40.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 231, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1171) [M::mem_pestat] mean and std.dev: (243.87, 176.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1506) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1144.589 CPU sec, 67.740 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (241, 944114, 93, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 116, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (115.13, 71.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.83, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 40, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 205) [M::mem_pestat] mean and std.dev: (41.95, 32.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 266) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 219, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (202.29, 94.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1125.647 CPU sec, 49.799 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (235, 943572, 77, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 113, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (118.88, 70.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 55, 2674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7960) [M::mem_pestat] mean and std.dev: (1091.51, 1732.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10603) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 238, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1214) [M::mem_pestat] mean and std.dev: (304.78, 260.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1565) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1140.400 CPU sec, 66.771 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (227, 943899, 84, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 103, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (119.28, 73.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 50, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (50.35, 56.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 243, 561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1319) [M::mem_pestat] mean and std.dev: (331.95, 289.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1698) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1127.745 CPU sec, 49.951 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (263, 944759, 74, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 114, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (121.26, 70.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 43, 79) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 191) [M::mem_pestat] mean and std.dev: (40.10, 26.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 247) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 228, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (186.65, 82.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1117.721 CPU sec, 49.363 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (241, 944714, 82, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 121, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (125.13, 73.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.33) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 48, 1600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4746) [M::mem_pestat] mean and std.dev: (772.69, 1356.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6319) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 279, 825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2071) [M::mem_pestat] mean and std.dev: (449.48, 418.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2694) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1164.065 CPU sec, 78.332 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (236, 943974, 103, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 132, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (127.75, 74.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 41, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 174) [M::mem_pestat] mean and std.dev: (39.18, 22.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 223) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 234, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (229.35, 123.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 735) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1138.799 CPU sec, 51.298 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (244, 944763, 89, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 99, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (116.80, 73.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 49, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 765) [M::mem_pestat] mean and std.dev: (65.27, 96.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (113, 215, 864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2366) [M::mem_pestat] mean and std.dev: (431.69, 480.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3117) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1141.154 CPU sec, 68.371 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (243, 945124, 83, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 113, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (117.38, 69.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 50, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (64.26, 94.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 247, 668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1660) [M::mem_pestat] mean and std.dev: (319.67, 338.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2156) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1123.266 CPU sec, 49.725 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (230, 944418, 99, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 97, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (109.70, 72.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.84, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 45, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 263) [M::mem_pestat] mean and std.dev: (39.51, 26.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 232, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (205.74, 106.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1144.063 CPU sec, 69.494 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (243, 944448, 84, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 106, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (117.93, 74.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 59, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2274) [M::mem_pestat] mean and std.dev: (249.28, 539.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3021) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 328, 936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2370) [M::mem_pestat] mean and std.dev: (669.32, 583.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3087) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1131.860 CPU sec, 50.075 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (231, 944621, 103, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 105, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (115.45, 74.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 45, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (47.45, 49.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 205, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 786) [M::mem_pestat] mean and std.dev: (223.60, 165.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1002) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1157.876 CPU sec, 76.255 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (260, 944449, 83, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 129, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (133.41, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 51, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1237) [M::mem_pestat] mean and std.dev: (112.75, 223.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1639) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (104, 220, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 641) [M::mem_pestat] mean and std.dev: (179.12, 106.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1130.304 CPU sec, 52.362 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (237, 944305, 70, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 99, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (111.86, 67.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 39, 1283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3801) [M::mem_pestat] mean and std.dev: (358.22, 845.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5060) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 278, 900) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2328) [M::mem_pestat] mean and std.dev: (462.61, 419.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3042) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1144.503 CPU sec, 69.023 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (247, 944170, 90, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 111, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (123.26, 76.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 53, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (58.49, 68.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 941) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 241, 1077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2933) [M::mem_pestat] mean and std.dev: (497.60, 593.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3861) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1132.875 CPU sec, 50.515 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (227, 944040, 87, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 114, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (119.82, 73.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.21) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 54, 934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2724) [M::mem_pestat] mean and std.dev: (265.03, 638.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3619) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (100, 210, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2314) [M::mem_pestat] mean and std.dev: (468.79, 595.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3052) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1149.127 CPU sec, 72.274 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (259, 944885, 79, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 110, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (117.92, 79.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 50, 112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (52.54, 49.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 361) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 235, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (214.92, 115.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1123.433 CPU sec, 49.534 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (265, 944797, 86, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 131, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (132.48, 79.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 43, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 186) [M::mem_pestat] mean and std.dev: (37.96, 21.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 241) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 221, 958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2588) [M::mem_pestat] mean and std.dev: (516.18, 557.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3403) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1154.526 CPU sec, 76.283 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (256, 945937, 96, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 118, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (119.56, 67.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 44, 116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (42.55, 42.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 254, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1302) [M::mem_pestat] mean and std.dev: (303.69, 258.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1686) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1132.578 CPU sec, 52.237 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (250, 944319, 77, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 111, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (124.39, 78.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 47, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 594) [M::mem_pestat] mean and std.dev: (50.32, 47.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (127, 206, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (195.39, 133.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1144.355 CPU sec, 72.114 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (232, 944081, 87, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 113, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (119.68, 74.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.85, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 44, 87) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 211) [M::mem_pestat] mean and std.dev: (46.06, 38.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 273) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 295, 892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2288) [M::mem_pestat] mean and std.dev: (477.44, 444.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2986) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1127.701 CPU sec, 49.826 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (254, 944323, 82, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 109, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (119.87, 75.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 45, 74) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 168) [M::mem_pestat] mean and std.dev: (45.37, 30.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 215) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 228, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 584) [M::mem_pestat] mean and std.dev: (183.08, 76.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1148.280 CPU sec, 66.042 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (244, 943856, 92, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 103, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (118.93, 76.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 55, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 245) [M::mem_pestat] mean and std.dev: (56.73, 43.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 315) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 203, 978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2622) [M::mem_pestat] mean and std.dev: (417.00, 427.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3444) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1125.040 CPU sec, 49.579 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (222, 943540, 84, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 115, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (119.72, 68.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 44, 65) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 139) [M::mem_pestat] mean and std.dev: (39.25, 21.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 176) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 218, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (190.64, 90.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1134.880 CPU sec, 59.129 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (231, 944139, 76, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 113, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (120.75, 72.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 48, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (57.25, 76.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 233, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (194.37, 74.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1156.443 CPU sec, 78.889 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (244, 944071, 82, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 106, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (116.24, 73.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 65, 860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2512) [M::mem_pestat] mean and std.dev: (163.23, 418.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3338) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 245, 787) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1913) [M::mem_pestat] mean and std.dev: (456.65, 457.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2476) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1133.760 CPU sec, 50.184 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (258, 944465, 81, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 129, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (126.42, 71.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 44, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (47.67, 37.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 250, 1056) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2816) [M::mem_pestat] mean and std.dev: (449.87, 468.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3696) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1147.080 CPU sec, 69.011 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (220, 944297, 99, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 119, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (123.58, 71.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 58, 717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2071) [M::mem_pestat] mean and std.dev: (146.61, 298.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2748) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 242, 670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1660) [M::mem_pestat] mean and std.dev: (338.61, 327.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2155) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1134.902 CPU sec, 50.139 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (245, 944365, 76, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 120, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (125.96, 78.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 46, 2639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7867) [M::mem_pestat] mean and std.dev: (1066.42, 2024.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10481) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 241, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107) [M::mem_pestat] mean and std.dev: (252.22, 192.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1426) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1150.066 CPU sec, 70.629 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (259, 944479, 87, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 103, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (117.23, 73.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 45, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 261) [M::mem_pestat] mean and std.dev: (44.70, 34.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (112, 195, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 625) [M::mem_pestat] mean and std.dev: (167.97, 88.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1133.666 CPU sec, 49.875 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (277, 944541, 74, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 108, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (113.86, 70.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 48, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 186) [M::mem_pestat] mean and std.dev: (45.27, 29.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 239) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 213, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (202.46, 79.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1158.314 CPU sec, 76.728 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (230, 944755, 83, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 129, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (126.77, 76.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 52, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (75.44, 104.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 216, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (202.88, 59.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1134.541 CPU sec, 52.019 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (249, 944659, 86, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 122, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (124.04, 76.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 50, 108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272) [M::mem_pestat] mean and std.dev: (45.63, 38.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 354) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 223, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 720) [M::mem_pestat] mean and std.dev: (176.03, 80.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1136.719 CPU sec, 62.220 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (218, 944339, 80, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 123, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (129.52, 78.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 52, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1646) [M::mem_pestat] mean and std.dev: (89.24, 200.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2186) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 246, 735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1855) [M::mem_pestat] mean and std.dev: (415.90, 411.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2415) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1129.778 CPU sec, 49.733 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (261, 945036, 77, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 119, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (122.78, 70.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 43, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (40.46, 31.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 257, 954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2528) [M::mem_pestat] mean and std.dev: (475.83, 472.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3315) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1140.431 CPU sec, 68.829 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (260, 944099, 94, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 118, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (121.62, 73.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 40, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (52.82, 56.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 399) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 219, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (191.77, 77.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 749) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1130.785 CPU sec, 50.135 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (254, 944175, 93, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 105, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (114.39, 71.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 45, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1184) [M::mem_pestat] mean and std.dev: (81.68, 169.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1568) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 233, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1183) [M::mem_pestat] mean and std.dev: (300.86, 245.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1523) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1125.595 CPU sec, 53.565 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (239, 944316, 90, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 114, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (118.47, 71.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 41, 75) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 175) [M::mem_pestat] mean and std.dev: (40.74, 25.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 225) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 266, 627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1455) [M::mem_pestat] mean and std.dev: (318.94, 251.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1161.487 CPU sec, 77.022 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (267, 944753, 75, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 108, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (119.50, 75.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.71, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 52, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (64.79, 69.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (120, 205, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1599) [M::mem_pestat] mean and std.dev: (313.51, 339.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2092) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1135.871 CPU sec, 52.235 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (211, 944018, 81, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 112, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (119.46, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.86, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 51, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 268) [M::mem_pestat] mean and std.dev: (49.66, 43.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 349) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 234, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (211.38, 83.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1140.782 CPU sec, 66.361 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (251, 944301, 76, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 108, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (119.19, 70.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 43, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (51.89, 55.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 237, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (195.36, 79.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1135.941 CPU sec, 50.111 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (238, 945231, 64, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 115, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (120.62, 78.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 39, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (48.21, 53.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 227, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (202.58, 91.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1142.807 CPU sec, 69.340 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (234, 943702, 108, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 122, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (128.41, 78.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 45, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (56.50, 78.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 256, 772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1972) [M::mem_pestat] mean and std.dev: (423.09, 397.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2572) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1130.407 CPU sec, 49.871 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (243, 945226, 91, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 105, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (116.67, 67.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 42, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 165) [M::mem_pestat] mean and std.dev: (39.25, 25.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 212) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 261, 768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1898) [M::mem_pestat] mean and std.dev: (444.16, 401.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2463) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1159.666 CPU sec, 76.544 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (239, 945049, 83, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 107, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (116.18, 70.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 49, 757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2205) [M::mem_pestat] mean and std.dev: (132.15, 270.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2929) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 228, 862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2290) [M::mem_pestat] mean and std.dev: (427.65, 518.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3004) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1136.786 CPU sec, 51.907 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (245, 944326, 67, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 126, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (124.42, 67.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 44, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 205) [M::mem_pestat] mean and std.dev: (42.09, 29.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 266) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 254, 577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1401) [M::mem_pestat] mean and std.dev: (293.46, 234.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1813) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1144.072 CPU sec, 64.867 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (225, 944439, 99, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 121, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (126.66, 75.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.85, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 54, 1704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5046) [M::mem_pestat] mean and std.dev: (756.42, 1396.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6717) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 224, 620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1554) [M::mem_pestat] mean and std.dev: (291.80, 277.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2021) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1121.930 CPU sec, 49.438 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (252, 944835, 83, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 106, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (121.42, 81.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 51, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 183) [M::mem_pestat] mean and std.dev: (47.40, 27.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 233) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (115, 278, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1417) [M::mem_pestat] mean and std.dev: (311.51, 254.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1851) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1139.809 CPU sec, 69.739 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (250, 944313, 85, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 112, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (115.88, 69.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.69, 38.31) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 48, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (58.93, 73.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 279, 917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2385) [M::mem_pestat] mean and std.dev: (481.09, 496.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3119) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1132.715 CPU sec, 50.062 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (219, 943736, 76, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 110, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (114.71, 67.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 41, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (47.15, 54.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 220, 774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2024) [M::mem_pestat] mean and std.dev: (375.60, 433.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2649) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1124.193 CPU sec, 49.498 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (229, 944877, 81, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 107, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (114.33, 73.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.86, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 51, 817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2383) [M::mem_pestat] mean and std.dev: (164.26, 418.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3166) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 246, 666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1706) [M::mem_pestat] mean and std.dev: (371.88, 355.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2226) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1151.935 CPU sec, 77.929 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (233, 944359, 71, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 107, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (121.21, 74.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.83, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 47, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 178) [M::mem_pestat] mean and std.dev: (37.26, 22.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 229) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 256, 651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1613) [M::mem_pestat] mean and std.dev: (389.40, 369.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2094) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1139.060 CPU sec, 51.573 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (266, 945213, 93, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 105, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (115.75, 72.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.68, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 45, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (51.08, 56.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 255, 692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1800) [M::mem_pestat] mean and std.dev: (344.30, 342.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2354) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1144.351 CPU sec, 68.132 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (259, 944772, 83, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 111, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (117.62, 70.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 54, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1452) [M::mem_pestat] mean and std.dev: (86.29, 163.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1927) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 225, 641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1573) [M::mem_pestat] mean and std.dev: (288.19, 265.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2039) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1130.093 CPU sec, 49.990 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (243, 945201, 62, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 116, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (124.36, 74.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 45, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (48.20, 50.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 224, 779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2029) [M::mem_pestat] mean and std.dev: (425.44, 477.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2654) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1153.178 CPU sec, 73.131 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (252, 944059, 90, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 119, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (124.17, 71.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 46, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 166) [M::mem_pestat] mean and std.dev: (42.56, 27.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 213) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 217, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1445) [M::mem_pestat] mean and std.dev: (340.03, 316.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1875) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1132.636 CPU sec, 49.970 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (257, 944508, 99, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 106, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (116.53, 70.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 44, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (52.71, 79.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 904) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 202, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 665) [M::mem_pestat] mean and std.dev: (183.66, 100.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1161.299 CPU sec, 77.196 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (247, 944305, 72, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 112, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (123.60, 83.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 43, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (62.18, 77.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 293, 1121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2945) [M::mem_pestat] mean and std.dev: (671.91, 659.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3857) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1133.390 CPU sec, 52.030 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (251, 944989, 78, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 106, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (118.68, 75.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 44, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (55.22, 59.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 295, 805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1965) [M::mem_pestat] mean and std.dev: (443.70, 389.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2545) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1143.596 CPU sec, 69.173 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (218, 943767, 89, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 124, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (127.82, 72.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 53, 1273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3761) [M::mem_pestat] mean and std.dev: (379.32, 855.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5005) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 250, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (205.58, 79.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1130.839 CPU sec, 49.969 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (247, 944815, 75, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 106, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (120.21, 77.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 45, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 151) [M::mem_pestat] mean and std.dev: (39.05, 25.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 192) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 231, 608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1510) [M::mem_pestat] mean and std.dev: (303.42, 273.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1961) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1137.085 CPU sec, 67.924 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (269, 944336, 80, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 117, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (119.75, 72.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 47, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 600) [M::mem_pestat] mean and std.dev: (52.81, 63.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 791) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 250, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107) [M::mem_pestat] mean and std.dev: (267.83, 222.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1426) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1133.191 CPU sec, 50.092 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (226, 944029, 95, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 99, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (106.33, 60.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 44, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1186) [M::mem_pestat] mean and std.dev: (87.80, 176.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1572) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (121, 200, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (187.06, 122.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1161.453 CPU sec, 76.987 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (244, 943997, 93, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 107, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (118.93, 71.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 44, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 219) [M::mem_pestat] mean and std.dev: (43.31, 30.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 282) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 243, 631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1579) [M::mem_pestat] mean and std.dev: (332.59, 321.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2053) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1136.054 CPU sec, 52.197 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (256, 943991, 95, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 122, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (123.54, 71.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 47, 86) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 196) [M::mem_pestat] mean and std.dev: (45.50, 29.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 251) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 296, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2925) [M::mem_pestat] mean and std.dev: (585.05, 599.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3831) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1140.064 CPU sec, 64.561 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (255, 944798, 83, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 121, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (121.11, 69.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 43, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 236) [M::mem_pestat] mean and std.dev: (48.04, 45.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 307) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (82, 176, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (143.13, 79.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1127.037 CPU sec, 49.668 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (257, 944085, 89, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 112, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (120.07, 69.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 54, 701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2045) [M::mem_pestat] mean and std.dev: (217.96, 446.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2717) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (121, 212, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1147) [M::mem_pestat] mean and std.dev: (280.59, 266.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1489) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1150.170 CPU sec, 69.917 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (247, 944840, 78, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 113, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (118.31, 70.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 43, 102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 254) [M::mem_pestat] mean and std.dev: (43.82, 32.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 397, 954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2410) [M::mem_pestat] mean and std.dev: (607.08, 568.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3138) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1122.952 CPU sec, 49.614 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (260, 944557, 77, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 116, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (121.56, 72.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 48, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249) [M::mem_pestat] mean and std.dev: (46.07, 35.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 322) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 205, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (216.62, 146.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1130.146 CPU sec, 58.294 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (251, 944641, 90, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 122, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (125.20, 76.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 57, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1167) [M::mem_pestat] mean and std.dev: (105.68, 188.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1545) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 261, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2014) [M::mem_pestat] mean and std.dev: (431.68, 469.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2631) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1149.766 CPU sec, 78.098 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (245, 944239, 81, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 110, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (119.29, 75.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 43, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (52.14, 54.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 223, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (195.00, 84.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1133.919 CPU sec, 50.421 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (261, 944262, 94, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 127, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (127.44, 72.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.34) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 53, 1707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5067) [M::mem_pestat] mean and std.dev: (865.11, 1496.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6850) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 235, 673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1657) [M::mem_pestat] mean and std.dev: (375.58, 348.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2149) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1140.677 CPU sec, 69.427 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (262, 944982, 88, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 111, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (123.09, 78.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 41, 92) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 222) [M::mem_pestat] mean and std.dev: (40.40, 27.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 287) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 203, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (219.86, 119.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1139.882 CPU sec, 50.368 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (257, 943981, 88, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 112, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (123.04, 75.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 44, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251) [M::mem_pestat] mean and std.dev: (44.39, 37.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (135, 206, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (188.66, 87.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1132.625 CPU sec, 67.933 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (237, 944831, 76, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 105, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (112.83, 73.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 48, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (52.07, 62.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (134, 229, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 716) [M::mem_pestat] mean and std.dev: (189.00, 92.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1122.365 CPU sec, 49.515 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (255, 944333, 78, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 104, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (116.10, 74.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 51, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (51.59, 48.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 221, 769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1927) [M::mem_pestat] mean and std.dev: (434.97, 450.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2506) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1156.323 CPU sec, 76.793 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (226, 943943, 81, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 120, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (127.22, 78.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.85, 38.20) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 49, 607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1765) [M::mem_pestat] mean and std.dev: (165.64, 402.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2344) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 240, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1457) [M::mem_pestat] mean and std.dev: (290.39, 267.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1893) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1131.061 CPU sec, 51.646 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (221, 944497, 90, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 104, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (110.15, 68.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.21) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 49, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (44.99, 30.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 225, 313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (204.81, 107.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1137.431 CPU sec, 69.258 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (219, 944358, 83, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 119, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (119.58, 74.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.17) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 52, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (56.49, 54.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 236, 900) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2328) [M::mem_pestat] mean and std.dev: (505.77, 537.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3042) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1127.627 CPU sec, 49.962 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (260, 944648, 90, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 131, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (133.38, 80.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.31) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 56, 678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1974) [M::mem_pestat] mean and std.dev: (170.42, 394.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2622) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 229, 751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1915) [M::mem_pestat] mean and std.dev: (416.36, 457.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2497) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1129.050 CPU sec, 64.294 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (248, 944536, 85, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 114, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (121.88, 75.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.68, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 42, 79) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 191) [M::mem_pestat] mean and std.dev: (42.43, 31.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 247) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 250, 702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1748) [M::mem_pestat] mean and std.dev: (373.49, 335.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2271) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1127.192 CPU sec, 49.624 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (246, 943254, 75, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 119, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (125.78, 77.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.86, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 48, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (76.75, 143.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1088) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 227, 874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2280) [M::mem_pestat] mean and std.dev: (475.37, 515.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2983) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1123.497 CPU sec, 52.558 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (253, 943935, 84, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 120, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (124.37, 72.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.91, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 52, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (76.27, 138.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 226, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 461) [M::mem_pestat] mean and std.dev: (209.57, 72.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1151.589 CPU sec, 76.254 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (235, 944329, 88, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 111, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (122.46, 75.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.34) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 42, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (55.58, 63.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 242, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (240.09, 119.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1131.367 CPU sec, 52.067 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (262, 944391, 79, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 90, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (105.84, 69.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.20) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 54, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (51.16, 40.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 244, 843) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2227) [M::mem_pestat] mean and std.dev: (431.92, 455.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2919) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1134.528 CPU sec, 63.613 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (214, 944517, 107, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 113, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (118.17, 67.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 51, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 252) [M::mem_pestat] mean and std.dev: (42.11, 25.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 326) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 267, 877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2307) [M::mem_pestat] mean and std.dev: (423.97, 458.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3022) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1127.611 CPU sec, 49.729 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (249, 944584, 84, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 111, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (119.42, 76.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.83, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 41, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 208) [M::mem_pestat] mean and std.dev: (37.43, 25.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 270) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (125, 284, 936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2558) [M::mem_pestat] mean and std.dev: (520.23, 603.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3369) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1123.107 CPU sec, 61.033 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (236, 943387, 87, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 107, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (113.56, 66.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 50, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (52.70, 43.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 218, 939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2533) [M::mem_pestat] mean and std.dev: (444.70, 532.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3330) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1126.325 CPU sec, 49.614 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (278, 944829, 94, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 108, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (117.02, 71.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.83, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 51, 88) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 212) [M::mem_pestat] mean and std.dev: (50.95, 41.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 274) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 255, 909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2399) [M::mem_pestat] mean and std.dev: (536.87, 614.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3144) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1154.438 CPU sec, 76.230 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (219, 944583, 79, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 111, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (118.35, 69.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 42, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251) [M::mem_pestat] mean and std.dev: (52.82, 47.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 233, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 602) [M::mem_pestat] mean and std.dev: (221.41, 105.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1126.490 CPU sec, 51.456 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (230, 944919, 65, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 104, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (115.57, 74.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 41, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 236) [M::mem_pestat] mean and std.dev: (44.04, 44.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 307) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 234, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2287) [M::mem_pestat] mean and std.dev: (329.83, 309.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2990) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1126.078 CPU sec, 67.962 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (252, 945048, 82, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 112, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (114.12, 67.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.34) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 53, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (50.87, 36.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 226, 576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1408) [M::mem_pestat] mean and std.dev: (322.89, 272.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1824) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1124.910 CPU sec, 49.767 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (245, 944273, 73, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 112, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (119.07, 67.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 47, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (47.70, 42.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 240, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (209.03, 77.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1119.726 CPU sec, 62.218 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (256, 944112, 82, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 118, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (125.45, 81.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 49, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (52.09, 48.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 278, 776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2002) [M::mem_pestat] mean and std.dev: (450.67, 417.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2615) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1118.698 CPU sec, 49.188 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (256, 945205, 80, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 122, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (118.73, 66.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 47, 75) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 163) [M::mem_pestat] mean and std.dev: (41.28, 23.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 207) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 229, 697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1797) [M::mem_pestat] mean and std.dev: (348.48, 334.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2347) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1104.101 CPU sec, 48.679 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (267, 944242, 85, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 108, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (115.30, 72.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 43, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 218) [M::mem_pestat] mean and std.dev: (38.25, 22.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 282) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 271, 1040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2718) [M::mem_pestat] mean and std.dev: (538.38, 588.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3557) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1140.847 CPU sec, 77.358 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (246, 944359, 104, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 115, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (117.75, 69.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.21) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 41, 70) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 164) [M::mem_pestat] mean and std.dev: (37.32, 22.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 211) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 211, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1928) [M::mem_pestat] mean and std.dev: (361.39, 452.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2524) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1121.993 CPU sec, 50.068 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (234, 944717, 96, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 116, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (126.16, 79.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 39, 81) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 199) [M::mem_pestat] mean and std.dev: (34.75, 23.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 258) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 222, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1925) [M::mem_pestat] mean and std.dev: (381.08, 422.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2521) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1121.465 CPU sec, 66.936 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (249, 943893, 87, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 106, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (115.42, 68.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 41, 99) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251) [M::mem_pestat] mean and std.dev: (41.03, 31.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 233, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1355) [M::mem_pestat] mean and std.dev: (291.80, 301.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1761) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1122.436 CPU sec, 49.429 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (220, 944673, 76, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 107, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (112.63, 66.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.84, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 49, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084) [M::mem_pestat] mean and std.dev: (62.64, 129.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1436) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 210, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (167.58, 65.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1124.373 CPU sec, 62.700 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (256, 944846, 94, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 116, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (124.09, 77.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.20) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 58, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1471) [M::mem_pestat] mean and std.dev: (80.64, 126.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1953) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 247, 719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1787) [M::mem_pestat] mean and std.dev: (369.56, 353.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2321) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1121.145 CPU sec, 49.394 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (253, 944961, 83, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 102, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (112.75, 70.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.31) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 47, 97) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 247) [M::mem_pestat] mean and std.dev: (45.82, 41.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 322) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 240, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1909) [M::mem_pestat] mean and std.dev: (341.68, 417.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2497) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1154.682 CPU sec, 76.616 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (245, 944748, 82, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 115, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (123.14, 68.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 43, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 182) [M::mem_pestat] mean and std.dev: (41.71, 22.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 233) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 225, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1340) [M::mem_pestat] mean and std.dev: (313.37, 292.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1740) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1118.132 CPU sec, 51.349 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (246, 943900, 89, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 118, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (121.03, 74.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 43, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (55.93, 80.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 230, 876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2362) [M::mem_pestat] mean and std.dev: (425.26, 472.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3105) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1122.446 CPU sec, 58.990 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (245, 943662, 79, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 125, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (128.81, 75.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 38, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 204) [M::mem_pestat] mean and std.dev: (34.90, 19.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 264) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 219, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (203.03, 120.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1117.324 CPU sec, 49.141 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (266, 944112, 78, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 122, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (127.68, 78.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 44, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (54.41, 62.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 199, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (168.06, 79.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1132.268 CPU sec, 66.125 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (250, 944942, 99, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 105, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (117.93, 76.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 49, 1545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4593) [M::mem_pestat] mean and std.dev: (901.02, 1518.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6976) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 244, 603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1447) [M::mem_pestat] mean and std.dev: (377.82, 326.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1121.517 CPU sec, 49.640 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (251, 944755, 71, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 115, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (116.88, 70.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.83, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 45, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 677) [M::mem_pestat] mean and std.dev: (66.68, 93.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 207, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (188.75, 84.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1141.863 CPU sec, 75.738 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (265, 944751, 78, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 119, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (115.79, 67.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 42, 102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (46.92, 51.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 345) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 230, 574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1328) [M::mem_pestat] mean and std.dev: (288.31, 258.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1705) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1121.789 CPU sec, 51.340 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (223, 943772, 84, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 117, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (125.91, 81.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.31) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 47, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (52.23, 55.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (120, 198, 676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1788) [M::mem_pestat] mean and std.dev: (317.79, 342.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2344) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1134.084 CPU sec, 67.344 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (246, 943942, 87, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 118, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (124.09, 77.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 38, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 223) [M::mem_pestat] mean and std.dev: (36.29, 26.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 289) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 268, 954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2454) [M::mem_pestat] mean and std.dev: (539.61, 540.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3204) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1128.051 CPU sec, 49.713 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (224, 944478, 66, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 114, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (120.62, 70.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 47, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 226) [M::mem_pestat] mean and std.dev: (45.85, 36.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 292) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 251, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1327) [M::mem_pestat] mean and std.dev: (319.91, 259.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1708) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1123.560 CPU sec, 67.105 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (246, 944718, 94, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 102, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (112.76, 74.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 46, 82) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 208) [M::mem_pestat] mean and std.dev: (44.57, 37.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 271) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 238, 788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1986) [M::mem_pestat] mean and std.dev: (466.09, 456.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2585) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1124.697 CPU sec, 49.601 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (247, 944786, 88, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 115, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (119.03, 68.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.21) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 54, 1505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4445) [M::mem_pestat] mean and std.dev: (458.81, 963.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5915) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 228, 658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1682) [M::mem_pestat] mean and std.dev: (308.63, 286.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2194) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1126.374 CPU sec, 57.302 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (264, 944569, 91, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 117, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (120.53, 69.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 43, 88) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 200) [M::mem_pestat] mean and std.dev: (43.93, 26.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 256) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 257, 665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1645) [M::mem_pestat] mean and std.dev: (333.06, 326.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2135) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1150.841 CPU sec, 78.599 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (262, 944045, 78, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 114, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (119.95, 73.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 43, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1140) [M::mem_pestat] mean and std.dev: (60.11, 116.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1513) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 259, 647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1625) [M::mem_pestat] mean and std.dev: (354.67, 313.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2114) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1126.040 CPU sec, 50.109 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (265, 944418, 79, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 119, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (122.46, 74.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 46, 75) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 163) [M::mem_pestat] mean and std.dev: (38.90, 19.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 207) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (109, 172, 1033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2881) [M::mem_pestat] mean and std.dev: (456.13, 581.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3805) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1125.470 CPU sec, 69.026 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (246, 944694, 64, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 112, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (122.72, 74.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 41, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (44.71, 47.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 265, 1027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2727) [M::mem_pestat] mean and std.dev: (601.16, 662.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3577) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1118.772 CPU sec, 49.305 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (247, 945077, 94, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 107, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (112.61, 70.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 44, 108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 260) [M::mem_pestat] mean and std.dev: (43.66, 32.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 233, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (190.97, 74.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1126.767 CPU sec, 61.962 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (256, 944603, 78, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 115, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (123.46, 73.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 49, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (63.25, 88.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 213, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (214.25, 141.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1123.048 CPU sec, 49.412 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (246, 944324, 87, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 105, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (118.35, 73.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 46, 99) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 237) [M::mem_pestat] mean and std.dev: (41.94, 25.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 306) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 232, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (208.75, 121.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1155.195 CPU sec, 76.385 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (259, 944110, 96, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 123, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (123.10, 71.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 45, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 190) [M::mem_pestat] mean and std.dev: (43.49, 34.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 245) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 236, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1503) [M::mem_pestat] mean and std.dev: (287.66, 262.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1947) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1126.879 CPU sec, 51.640 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (263, 944290, 96, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 99, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (116.58, 73.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 44, 900) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2636) [M::mem_pestat] mean and std.dev: (146.88, 378.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3504) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (112, 229, 750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2026) [M::mem_pestat] mean and std.dev: (431.51, 486.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2664) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1134.971 CPU sec, 67.619 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (242, 944669, 91, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (49, 97, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (109.94, 71.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 44, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (50.61, 69.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 265, 892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2296) [M::mem_pestat] mean and std.dev: (513.93, 504.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2998) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1121.192 CPU sec, 49.534 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (268, 944902, 72, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 118, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (120.15, 71.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.71, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 41, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (48.45, 46.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (130, 228, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045) [M::mem_pestat] mean and std.dev: (251.68, 189.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1350) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1124.728 CPU sec, 67.161 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (271, 944523, 90, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 121, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (125.19, 73.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 46, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251) [M::mem_pestat] mean and std.dev: (46.31, 42.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 326) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 250, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1301) [M::mem_pestat] mean and std.dev: (317.09, 267.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1669) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1130.022 CPU sec, 49.814 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (254, 943460, 94, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 104, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (114.02, 69.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 43, 870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2566) [M::mem_pestat] mean and std.dev: (232.56, 515.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3414) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 224, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (225.32, 143.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1110.096 CPU sec, 51.102 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (250, 944637, 94, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 108, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (119.72, 75.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 44, 97) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 245) [M::mem_pestat] mean and std.dev: (43.08, 35.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 319) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 241, 791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2059) [M::mem_pestat] mean and std.dev: (439.17, 464.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2693) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1147.613 CPU sec, 76.238 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (250, 944794, 83, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 114, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (123.31, 76.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 46, 95) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 231) [M::mem_pestat] mean and std.dev: (42.31, 35.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 299) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 216, 842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2196) [M::mem_pestat] mean and std.dev: (408.47, 434.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2873) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1124.731 CPU sec, 51.781 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (230, 944141, 85, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 127, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (125.35, 72.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 43, 74) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 174) [M::mem_pestat] mean and std.dev: (42.03, 26.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 224) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 221, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1056) [M::mem_pestat] mean and std.dev: (295.20, 256.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1355) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1131.777 CPU sec, 67.976 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (239, 944144, 74, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 109, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (121.32, 76.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 52, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (57.32, 50.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 217, 621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1601) [M::mem_pestat] mean and std.dev: (326.54, 325.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2091) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1119.478 CPU sec, 49.611 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (261, 944305, 83, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 106, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (119.18, 74.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 38, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 180) [M::mem_pestat] mean and std.dev: (36.88, 23.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 232) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 227, 746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1934) [M::mem_pestat] mean and std.dev: (431.29, 458.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2528) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1124.103 CPU sec, 60.986 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (228, 945138, 85, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 122, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (124.72, 68.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 41, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (47.30, 50.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 225, 582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1466) [M::mem_pestat] mean and std.dev: (313.45, 296.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1908) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1120.775 CPU sec, 49.361 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (234, 944618, 93, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 112, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (123.79, 79.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 53, 786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2302) [M::mem_pestat] mean and std.dev: (134.15, 272.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3060) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 242, 756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1956) [M::mem_pestat] mean and std.dev: (457.16, 512.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2556) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1143.892 CPU sec, 76.484 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (249, 943995, 91, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 119, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (127.71, 74.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.32) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 47, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 196) [M::mem_pestat] mean and std.dev: (39.46, 24.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 252) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 277, 1151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3105) [M::mem_pestat] mean and std.dev: (621.72, 656.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4082) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1119.441 CPU sec, 51.229 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (235, 944355, 84, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 127, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (129.38, 77.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.20) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 42, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (46.87, 47.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 195, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (162.35, 72.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1131.050 CPU sec, 67.525 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (241, 944155, 86, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 114, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (116.49, 71.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 40, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 172) [M::mem_pestat] mean and std.dev: (34.94, 22.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 222) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (114, 242, 664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1764) [M::mem_pestat] mean and std.dev: (302.44, 354.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2314) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1114.115 CPU sec, 49.211 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (237, 943876, 87, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 131, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (135.56, 75.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 42, 1600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4746) [M::mem_pestat] mean and std.dev: (719.16, 1338.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6319) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (122, 238, 607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1577) [M::mem_pestat] mean and std.dev: (327.29, 342.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2062) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1127.165 CPU sec, 64.088 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (221, 944317, 86, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 109, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (115.64, 67.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.84, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 50, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (65.23, 89.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1463) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 274, 1189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3181) [M::mem_pestat] mean and std.dev: (496.28, 518.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4177) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1121.497 CPU sec, 49.623 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (228, 944511, 75, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 108, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (123.72, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.86, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 55, 2191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6501) [M::mem_pestat] mean and std.dev: (921.29, 1517.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8656) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 183, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (164.95, 77.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1147.921 CPU sec, 76.115 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (255, 944974, 80, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 114, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (120.76, 74.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 39, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (49.94, 63.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 228, 832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2126) [M::mem_pestat] mean and std.dev: (414.00, 461.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1122.900 CPU sec, 51.207 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (224, 943634, 76, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 113, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (120.13, 72.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.20) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 48, 102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 254) [M::mem_pestat] mean and std.dev: (54.31, 52.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 214, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (193.52, 106.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1133.541 CPU sec, 68.253 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (262, 943806, 92, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 118, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (122.11, 71.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 50, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (45.33, 35.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 238, 793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2101) [M::mem_pestat] mean and std.dev: (384.11, 443.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2755) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1127.825 CPU sec, 50.017 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (227, 944152, 84, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 117, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (123.78, 75.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 42, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (51.15, 52.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 255, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (234.00, 59.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1133.160 CPU sec, 65.715 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (250, 944213, 76, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 115, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (122.11, 75.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.90, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 42, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (48.15, 47.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 267, 751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1903) [M::mem_pestat] mean and std.dev: (357.61, 318.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2479) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1120.942 CPU sec, 49.320 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (263, 944825, 84, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 119, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (124.61, 76.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 47, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1370) [M::mem_pestat] mean and std.dev: (93.93, 172.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1817) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 220, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (193.42, 74.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1125.593 CPU sec, 61.608 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (246, 943848, 90, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 109, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (119.10, 72.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 46, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (71.43, 103.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 216, 355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (201.82, 120.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1147.698 CPU sec, 77.013 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (254, 944325, 74, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 111, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (118.28, 74.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 47, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 267) [M::mem_pestat] mean and std.dev: (44.34, 33.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 348) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 244, 604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1494) [M::mem_pestat] mean and std.dev: (344.86, 318.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1939) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1122.635 CPU sec, 49.714 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (213, 943654, 87, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 113, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (118.13, 72.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 44, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 218) [M::mem_pestat] mean and std.dev: (47.15, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 282) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 242, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1079) [M::mem_pestat] mean and std.dev: (315.90, 293.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1492) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1134.026 CPU sec, 68.911 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (249, 944409, 106, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 110, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (114.80, 71.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 52, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (56.35, 59.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 250, 776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1938) [M::mem_pestat] mean and std.dev: (466.30, 423.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2519) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1125.113 CPU sec, 49.549 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (250, 944850, 82, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 112, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (120.25, 73.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 60, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (75.38, 90.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 239, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1326) [M::mem_pestat] mean and std.dev: (276.56, 240.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1720) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1126.454 CPU sec, 67.401 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (266, 944123, 92, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 117, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (124.83, 78.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 57, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (66.20, 66.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 249, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1450) [M::mem_pestat] mean and std.dev: (311.69, 254.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1889) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1125.688 CPU sec, 49.601 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (233, 945132, 74, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 114, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (121.86, 72.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.83, 38.31) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 50, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (49.58, 54.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 672) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 245, 679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1701) [M::mem_pestat] mean and std.dev: (358.50, 341.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2212) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1157.070 CPU sec, 76.148 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (253, 943992, 88, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 110, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (119.44, 74.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 41, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 262) [M::mem_pestat] mean and std.dev: (44.24, 39.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 234, 660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1690) [M::mem_pestat] mean and std.dev: (386.86, 413.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2205) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1131.603 CPU sec, 51.861 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (242, 943933, 94, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 108, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (114.99, 66.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 58, 949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2803) [M::mem_pestat] mean and std.dev: (225.65, 555.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3730) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 247, 723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1841) [M::mem_pestat] mean and std.dev: (411.68, 384.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2400) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1127.692 CPU sec, 64.992 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (252, 945495, 78, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 122, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (121.15, 71.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 48, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (56.55, 86.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1117) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 207, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (191.94, 92.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1125.640 CPU sec, 49.776 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (269, 944739, 97, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 107, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (118.81, 73.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 42, 74) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 172) [M::mem_pestat] mean and std.dev: (42.63, 28.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 221) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 239, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (218.50, 100.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1133.406 CPU sec, 64.814 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (227, 943981, 94, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 145, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (137.71, 75.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 52, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (57.86, 58.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 253, 823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2121) [M::mem_pestat] mean and std.dev: (418.00, 376.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2770) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1124.284 CPU sec, 49.570 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (259, 944944, 93, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 118, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (120.74, 74.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.20) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 46, 97) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 243) [M::mem_pestat] mean and std.dev: (42.12, 29.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 316) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 267, 1033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2653) [M::mem_pestat] mean and std.dev: (626.55, 631.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3463) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1125.053 CPU sec, 56.441 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (234, 944143, 77, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 113, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (114.22, 69.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 49, 817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2395) [M::mem_pestat] mean and std.dev: (202.11, 463.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3184) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 243, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1195) [M::mem_pestat] mean and std.dev: (282.17, 276.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1542) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1158.850 CPU sec, 76.344 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (246, 944359, 73, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 118, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (124.81, 74.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 42, 77) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 177) [M::mem_pestat] mean and std.dev: (37.71, 21.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 227) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 241, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000) [M::mem_pestat] mean and std.dev: (261.67, 205.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1284) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1137.017 CPU sec, 52.832 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (246, 945025, 90, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 124, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (127.80, 75.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.21) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 48, 82) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 186) [M::mem_pestat] mean and std.dev: (44.32, 27.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 238) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 253, 652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1574) [M::mem_pestat] mean and std.dev: (369.07, 316.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2035) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1142.573 CPU sec, 70.143 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (273, 944228, 69, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 121, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (125.17, 73.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.33) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 61, 767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2237) [M::mem_pestat] mean and std.dev: (178.51, 384.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2972) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 246, 652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1608) [M::mem_pestat] mean and std.dev: (339.16, 368.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2086) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1130.437 CPU sec, 50.191 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (247, 944309, 80, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 112, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (117.85, 73.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 46, 1532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4550) [M::mem_pestat] mean and std.dev: (579.81, 1170.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6059) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 244, 1086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2908) [M::mem_pestat] mean and std.dev: (555.03, 633.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3819) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1140.724 CPU sec, 67.428 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (253, 944318, 89, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 115, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (122.12, 76.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 46, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (50.78, 52.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 286, 902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2326) [M::mem_pestat] mean and std.dev: (473.82, 467.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3038) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1129.156 CPU sec, 50.045 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (235, 944114, 80, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 95, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (107.12, 68.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.92, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 52, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (71.57, 94.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 258, 916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2426) [M::mem_pestat] mean and std.dev: (494.42, 520.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3181) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1160.862 CPU sec, 77.654 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (245, 945164, 84, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 101, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (115.73, 70.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 47, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 164) [M::mem_pestat] mean and std.dev: (40.13, 20.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 210) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 234, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1524) [M::mem_pestat] mean and std.dev: (382.71, 393.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1978) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1128.954 CPU sec, 52.114 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (218, 944035, 78, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 121, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (125.95, 72.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.32) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 55, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1532) [M::mem_pestat] mean and std.dev: (128.17, 246.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2034) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (130, 241, 916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2488) [M::mem_pestat] mean and std.dev: (468.92, 478.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3274) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1146.379 CPU sec, 69.771 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (226, 944036, 83, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 120, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (123.36, 72.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 40, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 170) [M::mem_pestat] mean and std.dev: (34.34, 21.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 219) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 236, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (235.49, 148.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1067) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1134.469 CPU sec, 50.361 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (237, 944447, 98, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 121, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (126.38, 71.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 44, 816) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2392) [M::mem_pestat] mean and std.dev: (157.15, 390.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3180) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 202, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1182) [M::mem_pestat] mean and std.dev: (269.59, 216.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1145.366 CPU sec, 67.664 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (219, 943483, 71, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 106, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (115.05, 69.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 49, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (56.56, 68.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 238, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (216.58, 102.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1026) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1123.396 CPU sec, 49.516 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (249, 944605, 94, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 97, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (111.54, 71.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 50, 267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 751) [M::mem_pestat] mean and std.dev: (50.69, 58.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 993) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 247, 715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1771) [M::mem_pestat] mean and std.dev: (429.91, 451.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2299) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1112.406 CPU sec, 50.080 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (242, 944329, 82, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 127, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (129.50, 75.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.70, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 48, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 176) [M::mem_pestat] mean and std.dev: (40.78, 21.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 224) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (117, 243, 1051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2919) [M::mem_pestat] mean and std.dev: (538.29, 692.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3853) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1154.362 CPU sec, 76.607 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (265, 945137, 79, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 115, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (120.64, 73.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 53, 662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1922) [M::mem_pestat] mean and std.dev: (183.27, 409.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2552) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 226, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (202.06, 101.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1130.044 CPU sec, 52.405 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (237, 944438, 91, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 122, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (122.05, 75.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.21) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 54, 870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2548) [M::mem_pestat] mean and std.dev: (158.64, 386.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3387) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 251, 1125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3005) [M::mem_pestat] mean and std.dev: (594.53, 686.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3945) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1138.771 CPU sec, 65.444 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (252, 944610, 71, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 127, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (125.53, 72.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 50, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (64.88, 74.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (130, 216, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1231) [M::mem_pestat] mean and std.dev: (273.65, 253.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1598) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1123.472 CPU sec, 49.694 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (250, 944377, 85, 68) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 100, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (114.57, 72.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 51, 879) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2579) [M::mem_pestat] mean and std.dev: (190.43, 498.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3429) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 241, 822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2094) [M::mem_pestat] mean and std.dev: (399.44, 377.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2730) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1142.471 CPU sec, 66.369 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (251, 943944, 68, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 118, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (122.66, 74.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 37, 59) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 139) [M::mem_pestat] mean and std.dev: (34.25, 19.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 179) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 265, 931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2413) [M::mem_pestat] mean and std.dev: (497.31, 510.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3154) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1124.806 CPU sec, 49.676 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (267, 944363, 100, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 111, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (120.80, 78.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.20) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 53, 769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2245) [M::mem_pestat] mean and std.dev: (140.73, 332.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2983) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 252, 762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1958) [M::mem_pestat] mean and std.dev: (334.38, 340.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2556) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1158.251 CPU sec, 76.599 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (259, 944347, 84, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 111, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (119.38, 70.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 47, 903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2661) [M::mem_pestat] mean and std.dev: (227.41, 551.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3540) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 205, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (183.65, 83.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1137.966 CPU sec, 52.602 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (250, 944293, 66, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 128, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (126.44, 71.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 42, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (37.75, 36.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 445) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 237, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (215.23, 132.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1147.276 CPU sec, 66.905 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (255, 944404, 68, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 106, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (119.06, 80.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 56, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1648) [M::mem_pestat] mean and std.dev: (89.89, 143.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2189) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (120, 200, 373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (239.18, 199.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1132) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1122.043 CPU sec, 49.597 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (265, 944061, 97, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 115, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (123.81, 78.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 51, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (67.83, 88.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 824) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 228, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (242.04, 160.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1148.020 CPU sec, 68.996 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (224, 944379, 89, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 111, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (118.32, 70.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 47, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 166) [M::mem_pestat] mean and std.dev: (39.88, 26.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 213) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 225, 769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1999) [M::mem_pestat] mean and std.dev: (331.65, 309.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2614) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1123.223 CPU sec, 49.605 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (247, 944396, 81, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 98, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (109.29, 70.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.69, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 50, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 253) [M::mem_pestat] mean and std.dev: (49.22, 34.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 326) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 230, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1230) [M::mem_pestat] mean and std.dev: (288.58, 248.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1578) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1162.779 CPU sec, 76.866 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (263, 943903, 88, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 121, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (126.10, 73.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 44, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 269) [M::mem_pestat] mean and std.dev: (46.97, 35.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 348) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (108, 204, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (201.03, 137.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 828) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1134.114 CPU sec, 52.105 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (270, 944393, 83, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 102, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (113.74, 70.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 42, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (49.50, 47.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 275, 786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1922) [M::mem_pestat] mean and std.dev: (403.54, 363.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2490) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1145.178 CPU sec, 68.427 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (268, 944188, 80, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 105, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (115.37, 66.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 46, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (59.61, 67.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 832) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (112, 214, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 799) [M::mem_pestat] mean and std.dev: (180.77, 139.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1131.756 CPU sec, 50.244 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (229, 944294, 95, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 112, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (114.10, 66.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 46, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (65.56, 89.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 232, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (179.08, 75.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1145.375 CPU sec, 67.692 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (243, 944679, 91, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 119, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (126.33, 75.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 43, 1278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3788) [M::mem_pestat] mean and std.dev: (488.36, 937.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5043) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (120, 204, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (180.62, 106.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 796) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1135.401 CPU sec, 50.236 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (239, 944725, 101, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 124, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (133.38, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 54, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1132) [M::mem_pestat] mean and std.dev: (61.24, 95.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1500) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 222, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1463) [M::mem_pestat] mean and std.dev: (287.31, 270.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1145.489 CPU sec, 61.559 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (235, 944268, 86, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 115, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (120.85, 68.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 52, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (60.04, 66.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 254, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1787) [M::mem_pestat] mean and std.dev: (385.73, 355.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2314) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1156.798 CPU sec, 77.367 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (252, 944243, 87, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 116, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (120.24, 73.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 41, 75) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 173) [M::mem_pestat] mean and std.dev: (44.08, 30.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 222) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (114, 217, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (174.91, 83.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1133.834 CPU sec, 52.788 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (247, 944315, 88, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 114, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (122.47, 74.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 43, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (56.15, 55.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 251, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (200.19, 75.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 790) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1119.950 CPU sec, 54.444 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (235, 943815, 88, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 109, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (120.54, 77.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 53, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (44.65, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 476) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 234, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (241.28, 158.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1130.034 CPU sec, 49.675 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (259, 944214, 88, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 115, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (119.05, 73.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.21) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 47, 259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (57.13, 81.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 952) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (124, 203, 1006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2770) [M::mem_pestat] mean and std.dev: (391.70, 501.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3652) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1139.629 CPU sec, 70.062 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (243, 943635, 87, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 108, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (117.74, 73.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 58, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (59.54, 66.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 207, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (169.41, 72.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 789) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1136.218 CPU sec, 50.262 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (249, 944283, 106, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 103, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (112.05, 70.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 45, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 175) [M::mem_pestat] mean and std.dev: (39.83, 25.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 226) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 275, 885) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2323) [M::mem_pestat] mean and std.dev: (322.11, 294.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3042) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1148.582 CPU sec, 75.985 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (249, 944679, 100, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 115, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (115.29, 67.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 42, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 518) [M::mem_pestat] mean and std.dev: (54.33, 72.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 242, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (205.34, 100.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 829) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1132.158 CPU sec, 51.753 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (245, 944483, 104, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 105, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (119.09, 72.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 54, 701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2039) [M::mem_pestat] mean and std.dev: (131.87, 308.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2708) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 247, 673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1665) [M::mem_pestat] mean and std.dev: (397.40, 403.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2161) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1148.239 CPU sec, 68.062 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (253, 944225, 85, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 119, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (123.88, 70.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 41, 873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2567) [M::mem_pestat] mean and std.dev: (137.84, 382.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3414) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (135, 224, 836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2238) [M::mem_pestat] mean and std.dev: (457.21, 508.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2939) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1133.027 CPU sec, 50.141 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (237, 944581, 96, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 110, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (118.58, 71.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 49, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (48.99, 47.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 783) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 228, 808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2162) [M::mem_pestat] mean and std.dev: (411.52, 430.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2839) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1139.456 CPU sec, 69.967 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (227, 943725, 90, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 116, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (124.84, 75.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 46, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (48.76, 48.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 266, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2560) [M::mem_pestat] mean and std.dev: (560.73, 558.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3339) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1133.519 CPU sec, 49.945 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (237, 944103, 90, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 117, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (122.05, 71.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 43, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (48.93, 49.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 381) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 231, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (198.39, 103.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1133.573 CPU sec, 55.826 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (263, 944282, 78, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 114, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (120.17, 74.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.31) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 42, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (63.52, 97.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 209, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (180.77, 98.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1154.640 CPU sec, 76.530 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (246, 944042, 74, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 110, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (119.03, 72.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 48, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (71.84, 125.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1073) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 254, 939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2459) [M::mem_pestat] mean and std.dev: (574.59, 552.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3219) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1142.146 CPU sec, 52.344 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (210, 943762, 92, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 118, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (125.09, 78.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 52, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (55.01, 54.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 267, 824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2128) [M::mem_pestat] mean and std.dev: (464.57, 473.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2780) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1142.919 CPU sec, 70.169 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (251, 944318, 84, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 115, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (121.19, 73.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 40, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 156) [M::mem_pestat] mean and std.dev: (37.20, 22.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 200) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 286, 842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2102) [M::mem_pestat] mean and std.dev: (505.51, 445.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2732) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1132.337 CPU sec, 50.167 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (271, 944505, 81, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 117, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (117.64, 71.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 47, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1047) [M::mem_pestat] mean and std.dev: (83.71, 138.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1386) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (100, 208, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1639) [M::mem_pestat] mean and std.dev: (340.93, 365.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2152) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1139.734 CPU sec, 69.540 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (258, 944777, 84, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 121, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (120.75, 70.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.32) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 46, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (42.46, 37.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 285, 687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1725) [M::mem_pestat] mean and std.dev: (404.54, 383.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2244) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1129.966 CPU sec, 49.817 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (233, 943773, 88, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 115, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (124.05, 79.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 48, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 362) [M::mem_pestat] mean and std.dev: (54.74, 58.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (120, 213, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (194.53, 104.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 780) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1155.182 CPU sec, 76.432 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (248, 944290, 86, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 112, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (120.90, 70.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 43, 67) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 145) [M::mem_pestat] mean and std.dev: (40.44, 22.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 184) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 247, 776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1942) [M::mem_pestat] mean and std.dev: (372.60, 386.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2525) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1130.029 CPU sec, 52.096 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (242, 944271, 91, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 113, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (115.42, 68.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 47, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1401) [M::mem_pestat] mean and std.dev: (74.79, 110.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1859) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 220, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 663) [M::mem_pestat] mean and std.dev: (194.16, 74.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1148.773 CPU sec, 71.183 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (237, 944379, 89, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 111, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (120.93, 70.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 38, 70) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 172) [M::mem_pestat] mean and std.dev: (37.37, 26.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 223) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 241, 746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1910) [M::mem_pestat] mean and std.dev: (434.34, 448.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2492) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1126.889 CPU sec, 49.761 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (264, 944357, 88, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 114, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (115.55, 69.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 41, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 206) [M::mem_pestat] mean and std.dev: (39.64, 26.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 267) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 229, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1148) [M::mem_pestat] mean and std.dev: (275.91, 222.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1480) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1139.037 CPU sec, 69.865 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (268, 943839, 93, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 110, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (116.72, 72.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.83, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 54, 727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2117) [M::mem_pestat] mean and std.dev: (216.43, 437.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2812) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (106, 190, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 712) [M::mem_pestat] mean and std.dev: (182.35, 148.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 914) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1129.519 CPU sec, 49.907 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (240, 944591, 105, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 121, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (126.71, 71.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 45, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (46.01, 44.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 386) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 252, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (209.25, 79.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 680) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1118.036 CPU sec, 49.763 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (235, 944279, 84, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 120, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (122.52, 74.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 49, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 191) [M::mem_pestat] mean and std.dev: (44.04, 27.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 245) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 237, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1213) [M::mem_pestat] mean and std.dev: (316.08, 268.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1568) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1154.376 CPU sec, 76.206 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (267, 944688, 72, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 106, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (117.48, 74.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 47, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 261) [M::mem_pestat] mean and std.dev: (56.02, 48.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (84, 215, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (168.14, 97.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 908) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1131.980 CPU sec, 52.286 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (257, 945311, 72, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 117, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (125.94, 78.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 46, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (68.05, 101.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (110, 225, 729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1967) [M::mem_pestat] mean and std.dev: (343.31, 394.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2586) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1135.207 CPU sec, 60.543 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (262, 943836, 83, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 115, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (120.98, 76.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 52, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (55.49, 42.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 272, 930) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2446) [M::mem_pestat] mean and std.dev: (559.41, 581.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3204) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1130.747 CPU sec, 49.799 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (250, 944720, 89, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 114, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (118.79, 73.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 46, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (59.52, 73.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 820) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 218, 722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1820) [M::mem_pestat] mean and std.dev: (342.10, 328.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2369) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1136.104 CPU sec, 61.378 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (240, 944544, 87, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 120, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (129.16, 78.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.71, 38.32) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 55, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (94.46, 153.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1197) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 256, 971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2503) [M::mem_pestat] mean and std.dev: (412.94, 422.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3269) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1125.429 CPU sec, 49.538 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (260, 944358, 75, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 118, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (123.34, 71.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 44, 108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (45.26, 39.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 363) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (134, 272, 689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1799) [M::mem_pestat] mean and std.dev: (456.14, 504.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2475) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1158.678 CPU sec, 76.566 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (232, 943980, 80, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 121, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (126.48, 77.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 41, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (46.27, 49.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 221, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (214.77, 109.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 769) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1137.907 CPU sec, 52.324 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (256, 944660, 93, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 107, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (116.63, 72.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 47, 79) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 177) [M::mem_pestat] mean and std.dev: (42.32, 24.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 226) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 214, 666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1670) [M::mem_pestat] mean and std.dev: (320.77, 370.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2172) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1145.032 CPU sec, 70.951 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (254, 944547, 95, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 122, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (124.66, 77.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 44, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (51.16, 66.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 289, 1085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2821) [M::mem_pestat] mean and std.dev: (591.90, 591.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3689) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1133.641 CPU sec, 50.018 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (310, 943626, 77, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 108, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (119.05, 76.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.31) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 42, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 250) [M::mem_pestat] mean and std.dev: (39.31, 30.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 326) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 225, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 520) [M::mem_pestat] mean and std.dev: (179.47, 75.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1123.005 CPU sec, 51.468 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (243, 944570, 109, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 113, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (119.87, 72.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 46, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 245) [M::mem_pestat] mean and std.dev: (50.86, 45.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 317) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 207, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (211.17, 136.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 890) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1122.572 CPU sec, 49.395 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (228, 943500, 93, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 105, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (121.92, 78.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 46, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (57.93, 77.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 253, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 592) [M::mem_pestat] mean and std.dev: (218.15, 106.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1160.542 CPU sec, 77.047 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (244, 944486, 87, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 106, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (115.86, 73.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 46, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (50.25, 51.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 248, 1497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4087) [M::mem_pestat] mean and std.dev: (681.29, 902.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5382) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1134.074 CPU sec, 52.115 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (247, 944190, 98, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 133, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (127.56, 73.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 53, 718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2100) [M::mem_pestat] mean and std.dev: (150.71, 334.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2791) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 216, 931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2493) [M::mem_pestat] mean and std.dev: (392.12, 530.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3274) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1129.435 CPU sec, 60.718 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (228, 943829, 88, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 109, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (116.18, 72.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 48, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (43.03, 33.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 657) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 233, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1978) [M::mem_pestat] mean and std.dev: (416.09, 461.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2584) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1125.007 CPU sec, 49.765 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (259, 944189, 79, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 112, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (120.13, 70.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 44, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (50.62, 47.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 365) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 234, 349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 681) [M::mem_pestat] mean and std.dev: (220.91, 86.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 847) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1148.344 CPU sec, 69.486 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (251, 945231, 73, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 110, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (117.17, 68.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 44, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (52.20, 49.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 243, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (228.15, 135.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1130.931 CPU sec, 50.038 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (259, 944517, 80, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 109, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (122.12, 71.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.31) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 53, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (57.52, 79.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (120, 254, 689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1827) [M::mem_pestat] mean and std.dev: (354.48, 404.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2396) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1132.093 CPU sec, 60.721 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (244, 944735, 74, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 122, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (125.13, 72.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 40, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (53.71, 54.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 374) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 260, 844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2212) [M::mem_pestat] mean and std.dev: (346.31, 343.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2896) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1145.141 CPU sec, 74.083 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (227, 944133, 93, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 106, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (115.76, 68.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 51, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 686) [M::mem_pestat] mean and std.dev: (58.93, 69.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 906) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 223, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 783) [M::mem_pestat] mean and std.dev: (212.43, 131.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 994) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1129.432 CPU sec, 50.043 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (245, 944245, 91, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 109, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (119.46, 74.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.87, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 47, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (57.90, 60.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 238, 737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1891) [M::mem_pestat] mean and std.dev: (342.72, 358.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2468) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1135.583 CPU sec, 69.930 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (235, 944574, 80, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 108, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (113.76, 71.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 44, 85) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 209) [M::mem_pestat] mean and std.dev: (36.18, 26.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 271) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 244, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (207.47, 113.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1132.306 CPU sec, 49.926 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (224, 944579, 69, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 128, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (125.75, 74.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.31) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 51, 88) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 208) [M::mem_pestat] mean and std.dev: (47.32, 36.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 268) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 257, 706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1764) [M::mem_pestat] mean and std.dev: (374.37, 353.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2293) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1136.981 CPU sec, 68.628 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (232, 944476, 77, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 120, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (129.98, 78.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 43, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (50.23, 56.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 464) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 225, 975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2507) [M::mem_pestat] mean and std.dev: (537.35, 556.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3273) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1130.761 CPU sec, 49.820 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (220, 944885, 79, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 107, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (120.16, 74.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 48, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (47.05, 34.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 245, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1379) [M::mem_pestat] mean and std.dev: (258.95, 244.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1789) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1156.370 CPU sec, 76.525 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (243, 944102, 89, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 126, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (125.76, 70.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 40, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (54.51, 63.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 202, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (172.94, 72.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1139.697 CPU sec, 52.244 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (232, 944927, 99, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 110, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (119.79, 69.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.32) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 48, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (45.96, 49.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 225, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1292) [M::mem_pestat] mean and std.dev: (275.62, 204.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1668) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1135.953 CPU sec, 67.996 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (229, 944321, 86, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 120, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (127.22, 74.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 47, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (53.84, 58.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 230, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (191.45, 68.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1128.914 CPU sec, 49.948 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (256, 944238, 95, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 112, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (119.83, 76.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 46, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 192) [M::mem_pestat] mean and std.dev: (43.68, 28.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 246) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 234, 1022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2736) [M::mem_pestat] mean and std.dev: (459.76, 500.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3593) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1130.033 CPU sec, 67.732 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (219, 944501, 92, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 103, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (112.75, 71.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 53, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259) [M::mem_pestat] mean and std.dev: (47.86, 38.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 204, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (229.91, 188.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1186) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1128.376 CPU sec, 49.791 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (270, 944523, 87, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 124, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (125.02, 70.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 46, 102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 258) [M::mem_pestat] mean and std.dev: (40.37, 26.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (120, 210, 782) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2106) [M::mem_pestat] mean and std.dev: (298.06, 372.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2768) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1112.964 CPU sec, 54.156 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (239, 943792, 83, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 109, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (119.91, 73.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.84, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 56, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (58.77, 71.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 255, 845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2175) [M::mem_pestat] mean and std.dev: (446.11, 521.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2840) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1147.107 CPU sec, 76.145 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (222, 943507, 96, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 105, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (114.16, 69.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 49, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (49.95, 42.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (130, 204, 638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1654) [M::mem_pestat] mean and std.dev: (372.67, 394.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2162) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1127.162 CPU sec, 51.683 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (266, 943780, 76, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 121, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (121.91, 71.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.32) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 41, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (74.75, 122.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 277, 837) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2165) [M::mem_pestat] mean and std.dev: (319.15, 268.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2829) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1130.058 CPU sec, 67.990 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (260, 944257, 80, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 114, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (115.55, 67.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.83, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 52, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (45.11, 36.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 270, 885) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2335) [M::mem_pestat] mean and std.dev: (456.38, 413.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3060) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1127.538 CPU sec, 49.859 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (232, 944134, 99, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 116, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (120.96, 68.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 44, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 256) [M::mem_pestat] mean and std.dev: (37.19, 33.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 335) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 224, 737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1937) [M::mem_pestat] mean and std.dev: (440.24, 467.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2537) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1128.473 CPU sec, 66.378 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (266, 944118, 79, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 112, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (123.03, 75.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 60, 1005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2937) [M::mem_pestat] mean and std.dev: (369.51, 790.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3903) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 240, 729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1827) [M::mem_pestat] mean and std.dev: (371.61, 391.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2376) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1126.097 CPU sec, 49.617 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (242, 943767, 80, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 129, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (130.30, 75.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 47, 1009) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2969) [M::mem_pestat] mean and std.dev: (144.27, 389.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3949) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 238, 750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1940) [M::mem_pestat] mean and std.dev: (443.11, 490.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2535) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1157.114 CPU sec, 76.435 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (235, 944987, 92, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 127, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (125.83, 74.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 41, 74) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 178) [M::mem_pestat] mean and std.dev: (36.49, 22.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 230) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (122, 235, 954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2618) [M::mem_pestat] mean and std.dev: (533.83, 622.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3450) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1126.375 CPU sec, 51.778 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (216, 944760, 91, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 112, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (123.33, 74.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 47, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (53.66, 59.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 233, 847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2241) [M::mem_pestat] mean and std.dev: (467.12, 489.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2938) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1119.658 CPU sec, 56.725 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (248, 943845, 92, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 109, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (122.76, 74.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 49, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 220) [M::mem_pestat] mean and std.dev: (42.00, 23.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 282) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 209, 310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (202.09, 79.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1124.416 CPU sec, 49.577 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (246, 944150, 89, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 121, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (125.26, 74.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.83, 38.32) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 46, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (53.21, 54.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 253, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (201.92, 96.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1137.388 CPU sec, 66.946 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (251, 944736, 86, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 104, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (117.00, 77.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 39, 74) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 182) [M::mem_pestat] mean and std.dev: (37.57, 26.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 236) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 219, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (195.09, 68.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1128.339 CPU sec, 49.887 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (247, 944605, 73, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 122, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (124.55, 81.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 57, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (58.68, 50.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 277, 1118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3014) [M::mem_pestat] mean and std.dev: (451.78, 539.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3962) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1116.540 CPU sec, 49.291 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (280, 944734, 75, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 116, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (123.64, 73.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 44, 82) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 184) [M::mem_pestat] mean and std.dev: (42.47, 28.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 235) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 230, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (237.53, 157.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1146.233 CPU sec, 75.834 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (222, 944730, 81, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 110, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (119.13, 72.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 49, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (53.86, 51.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 691) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 216, 577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1425) [M::mem_pestat] mean and std.dev: (269.15, 245.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1849) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1126.010 CPU sec, 51.644 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (251, 944511, 84, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 133, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (129.38, 73.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.19) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 40, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 537) [M::mem_pestat] mean and std.dev: (50.54, 72.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 263, 1346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3652) [M::mem_pestat] mean and std.dev: (635.21, 718.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4805) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1132.047 CPU sec, 68.065 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (228, 944433, 84, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 110, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (111.58, 67.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 54, 839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2449) [M::mem_pestat] mean and std.dev: (138.75, 329.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3254) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 223, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069) [M::mem_pestat] mean and std.dev: (243.18, 197.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1371) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1127.706 CPU sec, 49.921 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (227, 944511, 94, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 120, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (128.76, 80.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 49, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (39.17, 31.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (132, 201, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (171.47, 71.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1140.496 CPU sec, 69.343 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (237, 945032, 87, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 108, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (117.99, 69.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 49, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 171) [M::mem_pestat] mean and std.dev: (41.19, 31.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 221) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 234, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (192.42, 91.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1126.139 CPU sec, 49.623 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (222, 943994, 98, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 107, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (116.21, 72.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 46, 79) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 185) [M::mem_pestat] mean and std.dev: (39.90, 26.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 238) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 249, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 679) [M::mem_pestat] mean and std.dev: (230.00, 118.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1154.884 CPU sec, 76.415 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (257, 944828, 100, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (47, 91, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (107.41, 71.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.71, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 40, 78) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 184) [M::mem_pestat] mean and std.dev: (41.61, 33.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 237) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 263, 1045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2793) [M::mem_pestat] mean and std.dev: (485.05, 478.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3667) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1134.082 CPU sec, 52.263 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (255, 944112, 89, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 100, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (112.49, 69.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 38, 74) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 172) [M::mem_pestat] mean and std.dev: (41.52, 29.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 221) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 195, 691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1795) [M::mem_pestat] mean and std.dev: (372.62, 413.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2347) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1133.991 CPU sec, 67.779 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (255, 944978, 97, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 101, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (115.71, 73.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 53, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (52.22, 47.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 256, 892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2314) [M::mem_pestat] mean and std.dev: (492.09, 501.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3025) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1124.200 CPU sec, 49.841 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (265, 944551, 91, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 117, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (120.33, 69.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.31) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 53, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (54.80, 47.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 249, 771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2009) [M::mem_pestat] mean and std.dev: (448.28, 487.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2628) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1130.231 CPU sec, 67.599 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (264, 944286, 95, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 122, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (128.56, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 48, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (51.49, 50.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 264, 1025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2725) [M::mem_pestat] mean and std.dev: (527.60, 501.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3575) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1127.673 CPU sec, 49.842 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (226, 944800, 96, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 102, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (118.39, 73.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 44, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 219) [M::mem_pestat] mean and std.dev: (44.54, 33.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 282) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (119, 220, 600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1562) [M::mem_pestat] mean and std.dev: (332.94, 348.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2043) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1155.029 CPU sec, 76.366 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (242, 943892, 96, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 119, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (126.38, 72.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 43, 64) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 138) [M::mem_pestat] mean and std.dev: (36.94, 17.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 175) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 246, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 658) [M::mem_pestat] mean and std.dev: (213.95, 123.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1126.182 CPU sec, 51.427 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (256, 944407, 81, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 119, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (122.31, 74.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.20) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 42, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 161) [M::mem_pestat] mean and std.dev: (42.05, 26.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 206) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 247, 1038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2692) [M::mem_pestat] mean and std.dev: (555.11, 629.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3519) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1125.597 CPU sec, 67.787 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (216, 945068, 99, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 113, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (117.77, 67.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 61, 2339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6955) [M::mem_pestat] mean and std.dev: (1054.97, 1748.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9263) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 190, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (165.35, 59.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1132.414 CPU sec, 50.255 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (239, 943991, 88, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 110, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (126.72, 79.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 51, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 302) [M::mem_pestat] mean and std.dev: (58.27, 56.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 269, 924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2448) [M::mem_pestat] mean and std.dev: (504.19, 508.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3210) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1129.698 CPU sec, 66.764 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (242, 944462, 80, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 108, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (119.74, 73.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.21) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 40, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 159) [M::mem_pestat] mean and std.dev: (34.52, 19.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 204) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 220, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (217.00, 138.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1126.361 CPU sec, 49.668 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (233, 944169, 90, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 98, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (113.03, 72.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 40, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (57.42, 79.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 264, 1154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3068) [M::mem_pestat] mean and std.dev: (582.65, 647.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4025) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1122.874 CPU sec, 59.558 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (245, 944511, 84, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 103, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (114.21, 71.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 50, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 241) [M::mem_pestat] mean and std.dev: (48.76, 34.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 311) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 236, 619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1541) [M::mem_pestat] mean and std.dev: (354.77, 351.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2002) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1153.438 CPU sec, 78.250 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (234, 945095, 83, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 109, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (119.31, 72.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 40, 64) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 144) [M::mem_pestat] mean and std.dev: (38.59, 27.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 184) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 266, 589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1465) [M::mem_pestat] mean and std.dev: (330.68, 330.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1128.071 CPU sec, 50.052 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (260, 944012, 78, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 106, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (118.33, 77.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 48, 85) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 211) [M::mem_pestat] mean and std.dev: (39.46, 26.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 274) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 292, 831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2041) [M::mem_pestat] mean and std.dev: (551.81, 513.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2646) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1132.812 CPU sec, 67.677 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (266, 945040, 94, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 116, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (120.16, 76.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 41, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (46.07, 48.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 386) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (121, 209, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (183.04, 112.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 713) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1124.792 CPU sec, 49.643 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (246, 943977, 89, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 114, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (120.79, 73.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.71, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 42, 74) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 170) [M::mem_pestat] mean and std.dev: (37.72, 21.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 218) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 256, 850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2166) [M::mem_pestat] mean and std.dev: (418.65, 397.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2824) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1133.965 CPU sec, 67.921 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (228, 944124, 78, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 114, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (125.67, 77.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 47, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 178) [M::mem_pestat] mean and std.dev: (43.80, 22.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 227) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (132, 232, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (196.42, 107.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1126.485 CPU sec, 49.779 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (271, 944691, 101, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 112, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (121.32, 77.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 37, 74) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 180) [M::mem_pestat] mean and std.dev: (38.93, 29.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 233) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 275, 758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1852) [M::mem_pestat] mean and std.dev: (430.29, 371.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2399) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1148.802 CPU sec, 76.244 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (251, 943498, 74, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 110, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (115.87, 71.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.31) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 45, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (48.87, 44.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 352) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (88, 199, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (205.96, 193.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1232) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1131.198 CPU sec, 52.122 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (256, 944350, 84, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 116, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (122.62, 72.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.86, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 45, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 220) [M::mem_pestat] mean and std.dev: (40.43, 30.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 285) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (132, 248, 581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1479) [M::mem_pestat] mean and std.dev: (331.14, 326.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1928) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1124.214 CPU sec, 61.475 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (228, 944117, 84, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 98, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (111.45, 69.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 62, 1285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3795) [M::mem_pestat] mean and std.dev: (463.07, 959.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5050) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 237, 718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1794) [M::mem_pestat] mean and std.dev: (324.94, 272.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2332) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1125.481 CPU sec, 49.532 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (255, 945497, 82, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 117, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (121.33, 68.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 46, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (37.46, 26.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 245, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (209.87, 85.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 772) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1128.392 CPU sec, 67.884 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (245, 944366, 95, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 109, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (114.93, 70.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 41, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (48.22, 54.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 222, 968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2600) [M::mem_pestat] mean and std.dev: (528.41, 601.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3416) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1124.989 CPU sec, 49.627 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (239, 944002, 73, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 128, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (129.39, 73.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.88, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 45, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 155) [M::mem_pestat] mean and std.dev: (41.13, 23.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 198) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (123, 173, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1137) [M::mem_pestat] mean and std.dev: (248.55, 235.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1475) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1117.947 CPU sec, 54.461 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (290, 944448, 77, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 111, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (117.97, 73.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.20) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 47, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (54.85, 60.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 230, 923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2449) [M::mem_pestat] mean and std.dev: (417.70, 479.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3212) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1150.354 CPU sec, 75.983 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (240, 944158, 101, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 123, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (126.61, 70.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 48, 1356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4016) [M::mem_pestat] mean and std.dev: (455.96, 1002.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5346) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 225, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (217.25, 114.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1113.900 CPU sec, 50.506 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (257, 943639, 71, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 99, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (118.91, 82.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 42, 81) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 193) [M::mem_pestat] mean and std.dev: (40.82, 31.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 249) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (135, 224, 1077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2961) [M::mem_pestat] mean and std.dev: (573.03, 729.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1127.367 CPU sec, 67.802 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (242, 944626, 91, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 118, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (118.86, 75.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 55, 801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2349) [M::mem_pestat] mean and std.dev: (199.31, 489.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3123) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 226, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1900) [M::mem_pestat] mean and std.dev: (410.50, 436.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2482) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1113.259 CPU sec, 49.086 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (247, 944828, 85, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 100, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (116.86, 76.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 46, 110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (43.63, 32.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 193, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (170.09, 61.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1101.149 CPU sec, 51.480 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (230, 944438, 102, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 121, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (120.75, 74.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 44, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (49.90, 45.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 382) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (134, 229, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1262) [M::mem_pestat] mean and std.dev: (289.73, 272.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1638) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1107.212 CPU sec, 48.684 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (254, 944505, 116, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 115, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (119.59, 75.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 43, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 202) [M::mem_pestat] mean and std.dev: (46.16, 36.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 261) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 232, 1360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3744) [M::mem_pestat] mean and std.dev: (571.28, 663.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4936) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1131.228 CPU sec, 75.460 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (253, 944233, 81, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 116, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (120.22, 70.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 48, 88) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 216) [M::mem_pestat] mean and std.dev: (44.99, 29.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 280) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 228, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084) [M::mem_pestat] mean and std.dev: (293.56, 235.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1386) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1111.526 CPU sec, 50.681 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (250, 943626, 86, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 106, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (121.14, 79.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 48, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (57.37, 56.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (125, 195, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (204.52, 139.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1125) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1111.848 CPU sec, 65.283 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (247, 943811, 106, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 102, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (113.51, 69.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 51, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (55.60, 59.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 268, 719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1787) [M::mem_pestat] mean and std.dev: (399.67, 382.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2321) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1112.861 CPU sec, 49.175 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (217, 943763, 87, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 106, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (118.83, 71.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 45, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1386) [M::mem_pestat] mean and std.dev: (92.71, 204.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1839) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (111, 204, 587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1539) [M::mem_pestat] mean and std.dev: (298.07, 331.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2015) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1116.648 CPU sec, 65.523 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (240, 944911, 61, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 117, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (119.03, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 37, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 173) [M::mem_pestat] mean and std.dev: (33.36, 23.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 225) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 237, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (189.78, 76.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 730) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1106.692 CPU sec, 48.771 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (254, 944102, 70, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 104, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (119.66, 77.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.31) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 44, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 519) [M::mem_pestat] mean and std.dev: (59.00, 74.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 685) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 303, 1399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3737) [M::mem_pestat] mean and std.dev: (654.24, 710.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4906) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1100.957 CPU sec, 48.435 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (253, 943623, 87, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 92, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (110.12, 69.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 54, 1670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4940) [M::mem_pestat] mean and std.dev: (858.04, 1451.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6663) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 269, 1295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3477) [M::mem_pestat] mean and std.dev: (681.38, 738.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4568) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1134.118 CPU sec, 75.733 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (239, 944009, 96, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 113, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (116.01, 74.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.32) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 45, 95) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 239) [M::mem_pestat] mean and std.dev: (44.88, 39.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 311) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 237, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 635) [M::mem_pestat] mean and std.dev: (201.44, 74.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 784) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1111.305 CPU sec, 50.731 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (251, 944257, 75, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 118, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (122.08, 71.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.83, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 52, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (63.95, 70.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 211, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (208.95, 118.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 929) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1110.748 CPU sec, 66.264 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (257, 944328, 76, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 102, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (112.84, 68.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 63, 697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2021) [M::mem_pestat] mean and std.dev: (157.02, 326.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2683) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 262, 1068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2834) [M::mem_pestat] mean and std.dev: (602.78, 656.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3717) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1107.178 CPU sec, 48.862 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (260, 944891, 75, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 112, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (123.06, 73.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 48, 82) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 200) [M::mem_pestat] mean and std.dev: (47.63, 35.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 259) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 214, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (195.53, 112.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 951) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1114.845 CPU sec, 66.533 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (256, 944511, 78, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 113, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (121.74, 75.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 45, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 184) [M::mem_pestat] mean and std.dev: (39.79, 26.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 236) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (130, 236, 1327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3721) [M::mem_pestat] mean and std.dev: (596.25, 743.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4918) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1104.122 CPU sec, 48.665 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (243, 944821, 84, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 114, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (117.47, 72.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 54, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (60.52, 62.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 247, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1263) [M::mem_pestat] mean and std.dev: (320.50, 253.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1622) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1118.734 CPU sec, 74.930 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (254, 943632, 77, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 108, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (119.03, 77.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 49, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (50.39, 46.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 208, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 741) [M::mem_pestat] mean and std.dev: (191.49, 129.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 945) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1112.583 CPU sec, 50.757 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (240, 944656, 91, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 109, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (120.00, 75.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 49, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 565) [M::mem_pestat] mean and std.dev: (56.10, 64.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 238, 324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (207.77, 76.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1107.278 CPU sec, 63.240 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (247, 944224, 91, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 121, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (123.78, 71.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 49, 900) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2628) [M::mem_pestat] mean and std.dev: (220.76, 526.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3492) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 221, 1305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3583) [M::mem_pestat] mean and std.dev: (639.24, 696.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4722) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1106.883 CPU sec, 48.852 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (253, 944529, 74, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 117, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (123.62, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 53, 1717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5091) [M::mem_pestat] mean and std.dev: (894.38, 1500.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6894) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 244, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1488) [M::mem_pestat] mean and std.dev: (358.00, 350.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1927) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1112.499 CPU sec, 63.249 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (274, 944437, 99, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 116, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (119.12, 69.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 42, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 169) [M::mem_pestat] mean and std.dev: (38.43, 25.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 218) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (135, 232, 844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2262) [M::mem_pestat] mean and std.dev: (368.55, 381.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2971) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1108.756 CPU sec, 48.818 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (210, 944122, 77, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 111, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (118.32, 77.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.71, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 42, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (63.52, 90.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 195, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 561) [M::mem_pestat] mean and std.dev: (207.47, 80.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1122.150 CPU sec, 75.319 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (247, 943753, 83, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 109, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (119.10, 69.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.32) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 40, 86) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 206) [M::mem_pestat] mean and std.dev: (39.88, 27.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 266) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 282, 622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1562) [M::mem_pestat] mean and std.dev: (335.52, 296.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2032) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1107.239 CPU sec, 50.421 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (246, 944103, 85, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 123, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (127.13, 76.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 46, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 223) [M::mem_pestat] mean and std.dev: (44.12, 33.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 288) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (111, 236, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020) [M::mem_pestat] mean and std.dev: (219.65, 182.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1323) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1106.674 CPU sec, 64.644 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (237, 944414, 79, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 110, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (117.49, 68.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 40, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 163) [M::mem_pestat] mean and std.dev: (38.29, 26.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 208) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 267, 878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2302) [M::mem_pestat] mean and std.dev: (471.72, 520.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3014) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1107.572 CPU sec, 48.825 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (250, 944954, 83, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 111, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (118.30, 71.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.80, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 42, 66) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 146) [M::mem_pestat] mean and std.dev: (37.74, 19.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 186) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 217, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 652) [M::mem_pestat] mean and std.dev: (173.77, 72.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1105.664 CPU sec, 62.852 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (217, 944281, 90, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 108, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (117.91, 71.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.27) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 45, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 262) [M::mem_pestat] mean and std.dev: (41.99, 33.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 228, 562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1348) [M::mem_pestat] mean and std.dev: (317.88, 292.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1741) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1104.247 CPU sec, 48.574 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (220, 944474, 88, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 108, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (111.95, 63.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 66, 1288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3798) [M::mem_pestat] mean and std.dev: (287.49, 673.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5053) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 251, 839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2139) [M::mem_pestat] mean and std.dev: (488.61, 497.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2789) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1103.338 CPU sec, 58.232 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (246, 944636, 92, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 102, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (112.58, 69.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.90, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 50, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1219) [M::mem_pestat] mean and std.dev: (71.74, 107.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1617) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 264, 696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1790) [M::mem_pestat] mean and std.dev: (340.05, 300.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2337) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1113.356 CPU sec, 74.278 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (232, 944565, 83, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 114, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (118.47, 73.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 48, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (56.16, 54.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 261, 646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1544) [M::mem_pestat] mean and std.dev: (397.77, 339.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1993) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1104.293 CPU sec, 49.354 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (243, 944054, 85, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 110, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (119.10, 73.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 46, 87) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 209) [M::mem_pestat] mean and std.dev: (40.73, 27.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 270) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 277, 937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2511) [M::mem_pestat] mean and std.dev: (458.48, 454.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3298) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1104.761 CPU sec, 60.424 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (276, 944173, 84, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 122, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (130.80, 75.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 40, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 186) [M::mem_pestat] mean and std.dev: (42.28, 27.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 239) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 232, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1500) [M::mem_pestat] mean and std.dev: (370.83, 333.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1937) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1108.563 CPU sec, 48.781 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (252, 944425, 104, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 118, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (121.13, 73.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.75, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 54, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (55.60, 58.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 250, 752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1882) [M::mem_pestat] mean and std.dev: (451.55, 466.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2447) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1108.743 CPU sec, 63.037 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (251, 944531, 76, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 117, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (126.11, 76.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 48, 119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (42.71, 30.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 407) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 243, 765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1981) [M::mem_pestat] mean and std.dev: (357.11, 361.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2589) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1105.726 CPU sec, 48.642 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (237, 943885, 88, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 122, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (122.75, 73.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 46, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (48.04, 45.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 230, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 542) [M::mem_pestat] mean and std.dev: (190.56, 83.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 673) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1128.070 CPU sec, 75.356 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (247, 944513, 84, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 119, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (124.28, 74.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 51, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (53.47, 64.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 239, 992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2700) [M::mem_pestat] mean and std.dev: (339.10, 358.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3554) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1112.199 CPU sec, 50.718 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (220, 945025, 96, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 121, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (124.51, 70.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.81, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 51, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (45.99, 36.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 277, 940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2420) [M::mem_pestat] mean and std.dev: (514.48, 536.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3160) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1111.330 CPU sec, 66.215 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (230, 943872, 96, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 100, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (114.29, 72.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.30) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 58, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (76.00, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (121, 250, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1162) [M::mem_pestat] mean and std.dev: (291.77, 254.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1509) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1109.703 CPU sec, 48.990 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (254, 944268, 89, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 115, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (119.17, 73.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 51, 692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2016) [M::mem_pestat] mean and std.dev: (84.43, 170.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2678) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 261, 919) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2449) [M::mem_pestat] mean and std.dev: (473.31, 510.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3214) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1106.888 CPU sec, 61.259 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (246, 944279, 99, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 113, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (120.37, 75.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 42, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (50.69, 75.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 241, 784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2000) [M::mem_pestat] mean and std.dev: (363.43, 304.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2608) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1107.102 CPU sec, 48.671 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (242, 944875, 79, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 124, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (132.55, 79.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.84, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 50, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 528) [M::mem_pestat] mean and std.dev: (57.63, 60.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 260, 988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2614) [M::mem_pestat] mean and std.dev: (537.62, 561.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3427) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1098.848 CPU sec, 53.015 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (276, 944881, 76, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 130, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (126.66, 73.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.83, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 50, 1119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3305) [M::mem_pestat] mean and std.dev: (498.66, 947.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4398) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 215, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 508) [M::mem_pestat] mean and std.dev: (193.73, 57.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1129.995 CPU sec, 75.073 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (247, 944316, 69, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 99, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (115.82, 70.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.85, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 41, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259) [M::mem_pestat] mean and std.dev: (38.26, 25.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (126, 215, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (197.80, 108.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1109.938 CPU sec, 50.778 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (260, 944408, 90, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 106, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (114.47, 68.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.74, 38.26) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 61, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (70.83, 72.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 235, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 684) [M::mem_pestat] mean and std.dev: (208.43, 92.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1111.558 CPU sec, 64.674 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (243, 943919, 76, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 122, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (126.35, 76.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.79, 38.21) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 47, 88) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 212) [M::mem_pestat] mean and std.dev: (49.65, 39.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 274) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 245, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 581) [M::mem_pestat] mean and std.dev: (227.49, 99.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1106.238 CPU sec, 48.710 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (253, 943736, 80, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 121, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (130.17, 79.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 56, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (59.29, 70.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 875) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 199, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (194.14, 103.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 768) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1111.958 CPU sec, 65.029 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (222, 945543, 93, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 104, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (118.04, 77.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.73, 38.32) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 48, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 144) [M::mem_pestat] mean and std.dev: (42.19, 20.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 182) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 337, 1691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4669) [M::mem_pestat] mean and std.dev: (819.14, 941.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6158) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1100.824 CPU sec, 48.445 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (228, 944101, 91, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 114, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (122.20, 75.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.70, 38.34) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 45, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (50.03, 48.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 776) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 253, 903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2409) [M::mem_pestat] mean and std.dev: (467.05, 499.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3162) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1142.871 CPU sec, 75.828 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (261, 945271, 79, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 111, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (117.85, 74.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.82, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 49, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 190) [M::mem_pestat] mean and std.dev: (43.98, 26.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 245) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 235, 659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1623) [M::mem_pestat] mean and std.dev: (366.33, 359.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2105) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1115.414 CPU sec, 51.214 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (279, 944363, 89, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 119, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (121.52, 71.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.24) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 44, 81) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 191) [M::mem_pestat] mean and std.dev: (39.29, 28.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 246) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 235, 670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1676) [M::mem_pestat] mean and std.dev: (393.92, 372.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2179) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1119.286 CPU sec, 66.780 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (257, 944635, 96, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 113, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (122.66, 78.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.78, 38.29) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 56, 1483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4379) [M::mem_pestat] mean and std.dev: (537.85, 1061.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5827) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (105, 169, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (145.00, 71.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1118.716 CPU sec, 49.605 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (234, 943977, 79, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 102, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (120.30, 78.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.72, 38.23) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 39, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (42.46, 42.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 295, 770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1988) [M::mem_pestat] mean and std.dev: (413.02, 372.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2597) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1120.929 CPU sec, 60.408 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (229, 944757, 86, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 108, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (120.94, 77.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.76, 38.28) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 48, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 209) [M::mem_pestat] mean and std.dev: (41.91, 36.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 272) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 234, 1067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2843) [M::mem_pestat] mean and std.dev: (536.67, 592.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3731) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1112.648 CPU sec, 48.978 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 151942 sequences (15194200 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (263, 944969, 80, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 115, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (120.13, 74.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.77, 38.20) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 45, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 366) [M::mem_pestat] mean and std.dev: (51.37, 50.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 227, 643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1577) [M::mem_pestat] mean and std.dev: (367.12, 341.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2044) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1102.546 CPU sec, 47.532 real sec [M::process] 0 single-end sequences; 151942 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 59627, 8, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 97, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (96.81, 56.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (335, 359, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 482) [M::mem_pestat] mean and std.dev: (360.96, 38.09) [M::mem_pestat] low and high boundaries for proper pairs: (188, 531) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 151942 reads in 70.983 CPU sec, 3.145 real sec [main] Version: 0.7.17-r1188 [main] CMD: /home/csyk2proj/tools/bwa.kit/bwa mem -p -t24 -R@RG\tID:KPGP-00253\tPL:ILLUMINA\tSM:KPGP-00253 -C /home/csyk2proj/data/bwa.kit/hs38DH.fa - [main] Real time: 55290.657 sec; CPU: 584857.170 sec