[M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (130, 938358, 67, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 139, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (131.28, 74.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 52, 1130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3332) [M::mem_pestat] mean and std.dev: (229.86, 523.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4433) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 247, 779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1955) [M::mem_pestat] mean and std.dev: (442.00, 374.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2543) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1227.908 CPU sec, 74.271 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (124, 939393, 71, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 124, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (122.34, 70.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 59, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (66.33, 64.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 223, 851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2269) [M::mem_pestat] mean and std.dev: (398.52, 446.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2978) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1189.185 CPU sec, 54.021 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (132, 939072, 70, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 116, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (117.16, 75.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.01, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 58, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (54.87, 41.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 321, 1119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2915) [M::mem_pestat] mean and std.dev: (520.56, 541.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3813) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1198.695 CPU sec, 70.708 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (124, 939551, 67, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 119, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (123.05, 72.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 59, 1521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4487) [M::mem_pestat] mean and std.dev: (745.86, 1349.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6142) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 266, 1270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3396) [M::mem_pestat] mean and std.dev: (643.97, 693.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4459) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1179.075 CPU sec, 51.953 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (134, 938747, 57, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 144, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (126.91, 73.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (347.99, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 54, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1118) [M::mem_pestat] mean and std.dev: (101.67, 176.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 330, 939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2395) [M::mem_pestat] mean and std.dev: (521.34, 414.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3123) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1201.822 CPU sec, 71.127 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 938699, 73, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 115, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (121.84, 72.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 59, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 570) [M::mem_pestat] mean and std.dev: (70.24, 85.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 748) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 224, 1388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3808) [M::mem_pestat] mean and std.dev: (730.09, 960.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5018) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1177.248 CPU sec, 52.022 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (108, 938896, 73, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 115, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (120.63, 76.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 69, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (73.97, 62.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 304, 984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2618) [M::mem_pestat] mean and std.dev: (536.03, 570.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3435) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1215.944 CPU sec, 72.524 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (122, 938826, 73, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 145, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (130.84, 72.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 55, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (68.40, 81.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 915) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 284, 657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1529) [M::mem_pestat] mean and std.dev: (383.00, 239.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1965) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1199.537 CPU sec, 54.702 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (135, 938873, 66, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 108, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (118.72, 64.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 55, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (60.12, 73.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 218, 606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1552) [M::mem_pestat] mean and std.dev: (352.63, 371.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2025) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1219.375 CPU sec, 73.378 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (117, 938988, 65, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 127, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (125.81, 70.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 54, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (57.96, 63.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 627, 2351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6641) [M::mem_pestat] mean and std.dev: (1128.74, 1271.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8786) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1191.103 CPU sec, 52.617 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (137, 939098, 74, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 133, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (130.89, 67.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 59, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (50.49, 47.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 262, 1109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2989) [M::mem_pestat] mean and std.dev: (582.83, 606.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3929) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1220.770 CPU sec, 73.783 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (136, 939612, 70, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 120, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (112.97, 65.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (347.98, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 57, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (68.07, 69.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 839) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 224, 782) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2062) [M::mem_pestat] mean and std.dev: (358.79, 420.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2702) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1190.916 CPU sec, 52.511 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (138, 938379, 85, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 123, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (114.11, 65.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 55, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (69.99, 88.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1115) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 281, 819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2089) [M::mem_pestat] mean and std.dev: (496.75, 408.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2724) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1204.739 CPU sec, 53.039 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (145, 937830, 62, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 134, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (127.09, 72.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 65, 2026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6010) [M::mem_pestat] mean and std.dev: (856.16, 1516.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8002) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 253, 975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2579) [M::mem_pestat] mean and std.dev: (484.58, 458.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3381) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1227.831 CPU sec, 75.503 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (105, 938632, 63, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 104, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (113.58, 72.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 67, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (86.16, 111.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 238, 979) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2581) [M::mem_pestat] mean and std.dev: (547.46, 518.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3382) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1203.959 CPU sec, 54.221 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (123, 938279, 91, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 113, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (113.22, 64.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.96) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 49, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 176) [M::mem_pestat] mean and std.dev: (39.93, 24.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 226) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 228, 904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2398) [M::mem_pestat] mean and std.dev: (407.56, 467.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3145) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1221.472 CPU sec, 72.782 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (137, 938419, 70, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 117, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (116.80, 63.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 59, 798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2312) [M::mem_pestat] mean and std.dev: (139.61, 302.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3069) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 282, 1058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2814) [M::mem_pestat] mean and std.dev: (561.48, 631.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3692) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1201.705 CPU sec, 52.941 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (136, 938558, 76, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 130, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (129.47, 74.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 53, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (51.75, 45.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 661, 1110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2986) [M::mem_pestat] mean and std.dev: (695.39, 681.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3924) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1219.277 CPU sec, 73.008 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (120, 938414, 90, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 140, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (129.55, 84.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 57, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (59.31, 64.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 333, 1359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3709) [M::mem_pestat] mean and std.dev: (733.30, 701.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4884) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1208.339 CPU sec, 53.237 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (125, 939561, 70, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 139, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (130.41, 93.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.94) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 61, 1441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4253) [M::mem_pestat] mean and std.dev: (588.63, 982.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5659) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 363, 1416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3854) [M::mem_pestat] mean and std.dev: (856.97, 976.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5073) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1244.740 CPU sec, 73.712 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 938444, 82, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 122, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (122.93, 76.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 79, 745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2153) [M::mem_pestat] mean and std.dev: (164.89, 286.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2857) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 549, 968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2508) [M::mem_pestat] mean and std.dev: (589.97, 452.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3278) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1199.639 CPU sec, 54.380 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (152, 939102, 68, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 127, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (124.40, 67.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.81) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 56, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (54.30, 55.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 532, 1537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4247) [M::mem_pestat] mean and std.dev: (990.53, 1098.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5602) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1227.514 CPU sec, 74.143 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 938563, 78, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 116, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (118.38, 71.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.83) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 62, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1039) [M::mem_pestat] mean and std.dev: (115.03, 196.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1374) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 274, 871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2299) [M::mem_pestat] mean and std.dev: (455.03, 443.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3013) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1203.596 CPU sec, 53.144 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (116, 938776, 86, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 110, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (109.52, 63.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 51, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (58.88, 60.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 273, 1106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2964) [M::mem_pestat] mean and std.dev: (582.34, 564.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3893) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1242.283 CPU sec, 75.748 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (121, 938574, 78, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 130, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (124.34, 71.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 56, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (55.58, 60.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 214, 485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1149) [M::mem_pestat] mean and std.dev: (287.27, 288.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1481) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1203.530 CPU sec, 52.990 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (144, 938480, 71, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 140, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (134.13, 70.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 58, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 241) [M::mem_pestat] mean and std.dev: (58.73, 45.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 309) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 330, 1094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2898) [M::mem_pestat] mean and std.dev: (696.00, 693.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3800) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1218.231 CPU sec, 54.902 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 938546, 84, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 121, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (121.00, 77.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.93) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 50, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (51.12, 51.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 507, 1180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3084) [M::mem_pestat] mean and std.dev: (617.03, 507.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4036) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1238.287 CPU sec, 73.934 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (131, 938680, 66, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 106, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (116.13, 69.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 45, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (63.89, 80.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 213, 884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2346) [M::mem_pestat] mean and std.dev: (417.62, 398.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3077) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1218.361 CPU sec, 55.635 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (125, 937926, 63, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 129, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (127.71, 75.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.93) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 76, 827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2405) [M::mem_pestat] mean and std.dev: (296.04, 504.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3194) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 569, 1178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3024) [M::mem_pestat] mean and std.dev: (734.78, 747.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3947) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1253.697 CPU sec, 75.162 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (121, 938241, 85, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 125, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (114.92, 69.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 62, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (61.71, 58.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 427, 1592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4404) [M::mem_pestat] mean and std.dev: (850.14, 936.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5810) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1207.048 CPU sec, 53.297 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (123, 939740, 79, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 122, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (116.98, 67.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 57, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (75.73, 75.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 233, 711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1797) [M::mem_pestat] mean and std.dev: (366.29, 338.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2340) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1237.920 CPU sec, 74.781 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (142, 938881, 78, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 130, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (126.07, 77.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 57, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (58.00, 49.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 246, 591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1425) [M::mem_pestat] mean and std.dev: (320.39, 273.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1842) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1209.993 CPU sec, 53.294 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (121, 938478, 67, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 124, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (123.00, 74.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 48, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 145) [M::mem_pestat] mean and std.dev: (41.66, 23.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 183) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 401, 846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2134) [M::mem_pestat] mean and std.dev: (492.11, 457.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2778) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1279.265 CPU sec, 74.916 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (117, 939235, 77, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 113, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (111.30, 63.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 63, 1823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5393) [M::mem_pestat] mean and std.dev: (820.12, 1356.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7178) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 698, 1094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2852) [M::mem_pestat] mean and std.dev: (642.46, 450.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3731) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1213.946 CPU sec, 55.304 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (150, 938573, 70, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 129, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (122.06, 68.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 61, 1465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4323) [M::mem_pestat] mean and std.dev: (410.68, 804.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5752) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 391, 1224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3230) [M::mem_pestat] mean and std.dev: (859.35, 941.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4623) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1247.910 CPU sec, 75.569 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (118, 938915, 63, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 117, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (122.72, 71.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 59, 718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2090) [M::mem_pestat] mean and std.dev: (206.56, 428.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2776) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 228, 684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1682) [M::mem_pestat] mean and std.dev: (407.14, 389.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2181) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1199.612 CPU sec, 53.064 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (118, 938390, 59, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 133, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (133.37, 71.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 64, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (69.17, 87.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 277, 919) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2433) [M::mem_pestat] mean and std.dev: (536.16, 485.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3190) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1240.854 CPU sec, 75.127 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (119, 939426, 66, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 119, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (116.46, 62.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 51, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (65.31, 74.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 261, 1437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3965) [M::mem_pestat] mean and std.dev: (675.39, 669.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5229) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1201.886 CPU sec, 52.954 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (125, 939132, 72, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 125, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (128.82, 83.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 65, 1873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5551) [M::mem_pestat] mean and std.dev: (764.81, 1330.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7390) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 215, 576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1436) [M::mem_pestat] mean and std.dev: (316.31, 307.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1866) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1218.704 CPU sec, 56.776 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (124, 938140, 80, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 116, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (123.10, 73.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.11, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 61, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 818) [M::mem_pestat] mean and std.dev: (61.54, 56.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 261, 829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2111) [M::mem_pestat] mean and std.dev: (428.56, 439.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2752) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1231.868 CPU sec, 75.803 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (120, 938806, 72, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 109, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (109.04, 68.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.14, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 49, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (54.98, 57.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 431) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 265, 1266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3416) [M::mem_pestat] mean and std.dev: (728.48, 729.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4491) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1203.691 CPU sec, 53.298 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (138, 938490, 56, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 111, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (116.36, 66.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 47, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (44.02, 37.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 546, 1386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3786) [M::mem_pestat] mean and std.dev: (790.21, 687.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4986) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1246.073 CPU sec, 73.217 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (137, 939541, 75, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 143, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (136.60, 73.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 59, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (67.58, 55.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 273, 760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1922) [M::mem_pestat] mean and std.dev: (395.41, 323.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2503) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1218.879 CPU sec, 53.684 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (144, 938716, 76, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 140, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (123.95, 74.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 66, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1603) [M::mem_pestat] mean and std.dev: (125.27, 249.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2123) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 248, 959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2493) [M::mem_pestat] mean and std.dev: (544.89, 531.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3260) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1258.633 CPU sec, 75.133 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (129, 938261, 73, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 140, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (125.07, 67.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 65, 1824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5388) [M::mem_pestat] mean and std.dev: (903.16, 1442.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7170) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 211, 678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1772) [M::mem_pestat] mean and std.dev: (325.34, 287.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2319) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1218.722 CPU sec, 53.649 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (135, 937901, 78, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 119, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (126.02, 77.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.12, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 51, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 179) [M::mem_pestat] mean and std.dev: (45.68, 24.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 227) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 248, 1055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2841) [M::mem_pestat] mean and std.dev: (409.73, 442.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3734) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1273.268 CPU sec, 74.768 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (127, 938919, 89, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 114, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (113.87, 65.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 62, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1048) [M::mem_pestat] mean and std.dev: (130.73, 226.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1385) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 248, 1418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3844) [M::mem_pestat] mean and std.dev: (840.55, 944.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5057) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1224.437 CPU sec, 55.470 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (121, 938626, 75, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 126, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (117.09, 61.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.01, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 59, 982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2880) [M::mem_pestat] mean and std.dev: (401.89, 782.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3829) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 543, 1156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3082) [M::mem_pestat] mean and std.dev: (632.41, 723.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4045) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1235.852 CPU sec, 73.765 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (126, 938451, 66, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 118, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (114.68, 66.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.11, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 67, 2632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7818) [M::mem_pestat] mean and std.dev: (1112.50, 1863.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10411) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 230, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1370) [M::mem_pestat] mean and std.dev: (321.21, 281.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1769) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1215.837 CPU sec, 53.795 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (156, 939006, 65, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 136, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 527) [M::mem_pestat] mean and std.dev: (125.34, 74.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.10, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 65, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (68.31, 56.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 716) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 250, 564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1298) [M::mem_pestat] mean and std.dev: (360.48, 283.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1665) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1245.877 CPU sec, 75.368 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (117, 939042, 65, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 119, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (116.48, 63.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 58, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 790) [M::mem_pestat] mean and std.dev: (71.14, 79.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1041) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 257, 1196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3190) [M::mem_pestat] mean and std.dev: (491.12, 437.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4187) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1222.499 CPU sec, 53.785 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 939346, 63, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 123, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (120.61, 67.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 53, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (70.11, 85.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 676) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 294, 1447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3993) [M::mem_pestat] mean and std.dev: (679.62, 682.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5266) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1230.250 CPU sec, 59.643 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (128, 938499, 74, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 142, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (131.39, 80.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 55, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (66.03, 76.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 770) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 352, 823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2051) [M::mem_pestat] mean and std.dev: (426.61, 320.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2665) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1271.508 CPU sec, 74.768 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (138, 938540, 84, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 127, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (119.71, 70.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 50, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (63.11, 84.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 746) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (125, 239, 811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2183) [M::mem_pestat] mean and std.dev: (459.63, 545.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1223.383 CPU sec, 55.755 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (143, 938779, 71, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 132, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (125.85, 66.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 48, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (46.52, 37.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 366, 1221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3331) [M::mem_pestat] mean and std.dev: (745.34, 818.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4386) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1245.512 CPU sec, 75.812 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (132, 938580, 76, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 120, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (119.08, 68.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 43, 914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2684) [M::mem_pestat] mean and std.dev: (248.74, 518.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3569) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 529, 1473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4003) [M::mem_pestat] mean and std.dev: (798.35, 740.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5268) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1216.650 CPU sec, 53.692 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (117, 938567, 79, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 133, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (126.83, 71.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 57, 700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2024) [M::mem_pestat] mean and std.dev: (235.93, 487.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2686) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 704, 1183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3035) [M::mem_pestat] mean and std.dev: (717.26, 511.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3961) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1248.128 CPU sec, 75.374 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (145, 938243, 75, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 119, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (134.89, 83.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 56, 931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2737) [M::mem_pestat] mean and std.dev: (226.27, 566.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3640) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 261, 988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2574) [M::mem_pestat] mean and std.dev: (395.17, 361.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3367) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1222.819 CPU sec, 53.828 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (121, 938531, 79, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 120, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (126.68, 78.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 64, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (57.08, 41.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 443) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 222, 890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2398) [M::mem_pestat] mean and std.dev: (514.40, 528.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3152) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1257.238 CPU sec, 74.233 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 938992, 91, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 128, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (122.55, 70.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (347.99, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 58, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1089) [M::mem_pestat] mean and std.dev: (110.69, 168.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1442) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 672, 1456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4020) [M::mem_pestat] mean and std.dev: (936.83, 1035.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5302) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1226.226 CPU sec, 56.056 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (139, 938325, 92, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 101, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (111.06, 77.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 54, 1971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5847) [M::mem_pestat] mean and std.dev: (918.77, 1539.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7785) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 339, 866) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2192) [M::mem_pestat] mean and std.dev: (484.44, 431.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2855) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1234.647 CPU sec, 74.048 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (119, 939204, 66, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 128, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (122.13, 70.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 63, 935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2731) [M::mem_pestat] mean and std.dev: (189.15, 449.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3629) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 337, 1482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4014) [M::mem_pestat] mean and std.dev: (873.58, 916.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5280) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1210.683 CPU sec, 53.330 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (127, 938389, 70, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 132, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (129.04, 71.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 48, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (52.11, 40.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 428) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 294, 792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2010) [M::mem_pestat] mean and std.dev: (486.19, 492.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2619) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1230.948 CPU sec, 74.624 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (139, 938663, 99, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 109, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (107.67, 67.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.10, 34.81) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 60, 1295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3807) [M::mem_pestat] mean and std.dev: (441.37, 885.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5063) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 266, 729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1777) [M::mem_pestat] mean and std.dev: (439.44, 438.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2301) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1211.718 CPU sec, 53.428 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 939040, 70, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 113, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (116.77, 75.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 60, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (89.54, 120.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 284, 765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1993) [M::mem_pestat] mean and std.dev: (426.38, 378.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2607) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1207.354 CPU sec, 60.240 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 937968, 72, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 137, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (129.86, 74.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.10, 34.82) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 64, 515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1471) [M::mem_pestat] mean and std.dev: (149.32, 276.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1949) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 236, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1501) [M::mem_pestat] mean and std.dev: (337.24, 342.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1955) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1260.327 CPU sec, 76.586 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (148, 938426, 66, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 136, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (126.39, 64.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 58, 86) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 192) [M::mem_pestat] mean and std.dev: (48.92, 27.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 245) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 452, 1160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3096) [M::mem_pestat] mean and std.dev: (597.67, 519.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4064) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1211.502 CPU sec, 53.538 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (124, 938650, 70, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 142, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (131.66, 65.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 44, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 168) [M::mem_pestat] mean and std.dev: (41.77, 25.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 214) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 277, 1354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3626) [M::mem_pestat] mean and std.dev: (730.86, 761.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4762) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1256.293 CPU sec, 75.696 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (131, 938061, 66, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 127, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (121.93, 74.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 45, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (70.68, 96.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1051) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 506, 919) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2293) [M::mem_pestat] mean and std.dev: (597.10, 492.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2980) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1210.522 CPU sec, 53.244 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 938671, 71, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 125, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (123.17, 76.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 53, 884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2582) [M::mem_pestat] mean and std.dev: (236.36, 566.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3431) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 296, 1429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3889) [M::mem_pestat] mean and std.dev: (763.04, 883.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5119) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1247.309 CPU sec, 74.306 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (119, 938637, 71, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 139, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (127.27, 72.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.11, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 46, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (42.67, 32.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 264, 1220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3230) [M::mem_pestat] mean and std.dev: (569.47, 595.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4235) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1212.272 CPU sec, 53.375 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 938465, 62, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 134, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (130.52, 83.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 53, 1635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4843) [M::mem_pestat] mean and std.dev: (715.38, 1355.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6447) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 295, 1199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3169) [M::mem_pestat] mean and std.dev: (711.39, 791.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4154) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1256.621 CPU sec, 74.970 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (118, 939019, 70, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 125, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (120.59, 67.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.10, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 61, 941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2749) [M::mem_pestat] mean and std.dev: (271.36, 611.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3653) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 484, 1765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4975) [M::mem_pestat] mean and std.dev: (968.32, 1114.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6580) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1219.524 CPU sec, 55.362 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 938862, 80, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 126, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (122.28, 74.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.83) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 71, 1081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3173) [M::mem_pestat] mean and std.dev: (394.83, 787.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4219) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 542, 1279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3445) [M::mem_pestat] mean and std.dev: (699.03, 642.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4528) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1258.764 CPU sec, 74.621 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 938649, 80, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 130, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 500) [M::mem_pestat] mean and std.dev: (133.77, 89.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.01, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 65, 1137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3337) [M::mem_pestat] mean and std.dev: (348.80, 757.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4437) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 725, 1430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3952) [M::mem_pestat] mean and std.dev: (892.30, 878.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5213) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1230.005 CPU sec, 54.324 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (128, 937933, 70, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 112, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (109.44, 64.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 64, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (66.06, 67.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 208, 895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2383) [M::mem_pestat] mean and std.dev: (425.21, 476.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3127) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1242.164 CPU sec, 75.906 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (125, 938722, 81, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 112, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (109.12, 67.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 53, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (53.00, 58.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 993) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 243, 953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2541) [M::mem_pestat] mean and std.dev: (488.41, 457.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3335) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1215.011 CPU sec, 53.590 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 938243, 71, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (48, 104, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (109.06, 69.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 54, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (69.05, 69.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 270, 777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2041) [M::mem_pestat] mean and std.dev: (483.10, 494.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2673) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1231.126 CPU sec, 62.529 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (144, 938311, 75, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 117, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (119.18, 79.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 49, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 359) [M::mem_pestat] mean and std.dev: (44.53, 25.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 545, 1589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4357) [M::mem_pestat] mean and std.dev: (993.46, 1000.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5741) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1247.630 CPU sec, 75.997 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (127, 938621, 73, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 107, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (109.85, 64.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 54, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (55.63, 45.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 474, 851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2105) [M::mem_pestat] mean and std.dev: (530.71, 435.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2732) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1222.891 CPU sec, 54.172 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (148, 939174, 74, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 132, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (128.40, 65.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 53, 87) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 207) [M::mem_pestat] mean and std.dev: (45.86, 28.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 267) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 752, 1621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4435) [M::mem_pestat] mean and std.dev: (911.37, 914.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5842) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1248.342 CPU sec, 75.674 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (148, 938582, 85, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 129, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (129.81, 87.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.80) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 47, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 174) [M::mem_pestat] mean and std.dev: (47.21, 31.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 221) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 322, 1086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2918) [M::mem_pestat] mean and std.dev: (618.06, 621.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3834) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1222.990 CPU sec, 53.863 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (115, 938290, 70, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 121, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (124.54, 64.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 59, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (47.87, 28.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 571, 1495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4037) [M::mem_pestat] mean and std.dev: (783.50, 758.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5308) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1250.171 CPU sec, 74.703 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (136, 938587, 91, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 120, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (121.90, 69.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 55, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (49.04, 36.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 670, 1264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3340) [M::mem_pestat] mean and std.dev: (696.14, 671.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4378) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1210.508 CPU sec, 53.261 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (117, 938359, 93, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 117, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (119.75, 71.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (347.98, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 61, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1229) [M::mem_pestat] mean and std.dev: (115.53, 189.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1626) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 472, 1174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3110) [M::mem_pestat] mean and std.dev: (776.61, 788.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4078) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1260.293 CPU sec, 74.659 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (142, 938661, 74, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 127, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (120.40, 69.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (347.99, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 63, 769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2219) [M::mem_pestat] mean and std.dev: (170.03, 390.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2944) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 279, 829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2093) [M::mem_pestat] mean and std.dev: (396.71, 357.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2725) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1222.943 CPU sec, 55.978 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (137, 938760, 79, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 129, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (130.51, 76.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 62, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (87.09, 119.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 256, 748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1924) [M::mem_pestat] mean and std.dev: (348.00, 354.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2512) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1244.998 CPU sec, 74.827 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 938934, 68, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 119, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (118.78, 81.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.13, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 64, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (69.77, 59.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 302, 1088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2910) [M::mem_pestat] mean and std.dev: (711.48, 800.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3915) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1216.603 CPU sec, 53.738 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (131, 938909, 75, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 133, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (123.72, 68.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 76, 2490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7378) [M::mem_pestat] mean and std.dev: (1202.85, 1852.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9822) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 238, 749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1921) [M::mem_pestat] mean and std.dev: (448.15, 468.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2507) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1236.270 CPU sec, 75.110 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (116, 938699, 69, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 129, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (122.87, 68.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.14, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 60, 1169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3439) [M::mem_pestat] mean and std.dev: (410.77, 828.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4574) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (254, 831, 1307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3413) [M::mem_pestat] mean and std.dev: (844.39, 644.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4466) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1213.353 CPU sec, 53.471 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 939367, 79, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 118, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (123.13, 70.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 49, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 660) [M::mem_pestat] mean and std.dev: (54.74, 56.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 250, 869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2219) [M::mem_pestat] mean and std.dev: (595.10, 607.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3025) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1230.089 CPU sec, 64.227 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (150, 938826, 71, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 115, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (120.57, 73.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 69, 2022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5990) [M::mem_pestat] mean and std.dev: (1041.74, 1585.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7974) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 280, 1323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3553) [M::mem_pestat] mean and std.dev: (692.03, 753.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4668) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1239.695 CPU sec, 75.410 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (116, 937977, 67, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 103, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (114.31, 76.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 65, 689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1997) [M::mem_pestat] mean and std.dev: (193.53, 375.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2651) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 270, 986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2548) [M::mem_pestat] mean and std.dev: (565.97, 576.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3329) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1217.027 CPU sec, 53.835 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 939237, 72, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 118, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (125.60, 70.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.82) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 50, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970) [M::mem_pestat] mean and std.dev: (67.23, 127.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1283) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 711, 1332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3510) [M::mem_pestat] mean and std.dev: (878.53, 792.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4599) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1239.981 CPU sec, 73.935 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (148, 938647, 78, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 132, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (121.96, 65.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.94) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 53, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (63.02, 72.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 283, 753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1873) [M::mem_pestat] mean and std.dev: (430.58, 349.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2433) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1218.913 CPU sec, 53.645 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (139, 938086, 80, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 131, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (123.22, 72.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 61, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (84.41, 140.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1271) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 279, 1325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3543) [M::mem_pestat] mean and std.dev: (696.38, 935.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4652) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1226.835 CPU sec, 74.583 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (123, 938427, 74, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 139, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (135.82, 78.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 60, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (65.60, 65.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 620, 1792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4968) [M::mem_pestat] mean and std.dev: (1122.94, 1229.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6556) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1214.224 CPU sec, 53.458 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (153, 938408, 72, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 132, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (117.34, 70.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.10, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 47, 614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1794) [M::mem_pestat] mean and std.dev: (136.73, 323.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2384) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 319, 1039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2773) [M::mem_pestat] mean and std.dev: (561.41, 556.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3640) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1261.006 CPU sec, 74.658 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (120, 938013, 71, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (94, 139, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (126.73, 65.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.94) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 49, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 222) [M::mem_pestat] mean and std.dev: (45.91, 29.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 285) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 475, 1318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3480) [M::mem_pestat] mean and std.dev: (885.93, 929.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4605) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1226.470 CPU sec, 55.583 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (143, 938093, 68, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 115, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (117.98, 72.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 52, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (55.35, 54.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 282, 797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1987) [M::mem_pestat] mean and std.dev: (435.80, 377.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2582) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1252.921 CPU sec, 75.862 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (115, 938536, 86, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 125, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (120.08, 71.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 57, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (65.26, 72.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 778) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 291, 1131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3071) [M::mem_pestat] mean and std.dev: (560.82, 559.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4041) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1214.116 CPU sec, 53.557 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 938465, 90, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 118, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (121.90, 75.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (347.97, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 59, 517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1479) [M::mem_pestat] mean and std.dev: (108.90, 228.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1960) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 234, 1508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4162) [M::mem_pestat] mean and std.dev: (733.59, 735.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5489) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1240.190 CPU sec, 75.282 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 938547, 67, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 108, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (124.34, 94.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 56, 1585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4679) [M::mem_pestat] mean and std.dev: (686.02, 1372.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6226) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 282, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2076) [M::mem_pestat] mean and std.dev: (443.78, 444.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2717) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1218.141 CPU sec, 53.788 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (129, 938768, 75, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 127, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (118.67, 68.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.10, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 60, 657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1891) [M::mem_pestat] mean and std.dev: (181.86, 324.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2508) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 296, 861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2093) [M::mem_pestat] mean and std.dev: (508.14, 454.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2709) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1241.553 CPU sec, 65.843 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (141, 938855, 78, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 141, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (129.24, 69.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 48, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1612) [M::mem_pestat] mean and std.dev: (151.65, 343.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2140) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 265, 1017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2747) [M::mem_pestat] mean and std.dev: (515.35, 543.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3612) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1238.616 CPU sec, 74.742 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (127, 938327, 80, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 132, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (123.13, 64.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.12, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 53, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (67.56, 110.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 245, 1245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3429) [M::mem_pestat] mean and std.dev: (606.68, 686.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4521) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1217.275 CPU sec, 53.673 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (127, 938335, 76, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 156, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (139.86, 87.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 95, 2447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7255) [M::mem_pestat] mean and std.dev: (986.21, 1634.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9659) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 261, 782) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2046) [M::mem_pestat] mean and std.dev: (394.97, 399.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2678) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1252.399 CPU sec, 75.457 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (126, 938490, 65, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 123, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (119.74, 67.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 57, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (72.33, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 230, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (191.96, 85.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1215.280 CPU sec, 53.500 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 938123, 67, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 128, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (121.98, 62.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 51, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1215) [M::mem_pestat] mean and std.dev: (64.90, 96.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1610) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 814, 1369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3687) [M::mem_pestat] mean and std.dev: (782.42, 643.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4846) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1246.056 CPU sec, 76.406 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (137, 938734, 66, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 132, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (123.86, 75.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 62, 631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1829) [M::mem_pestat] mean and std.dev: (92.12, 137.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2428) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 233, 837) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2253) [M::mem_pestat] mean and std.dev: (415.74, 427.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2961) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1215.733 CPU sec, 53.500 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (150, 938674, 79, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 145, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (130.97, 69.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 61, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (101.51, 156.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1227) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 241, 945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2463) [M::mem_pestat] mean and std.dev: (425.53, 419.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3222) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1201.074 CPU sec, 52.964 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (137, 938721, 75, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 115, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (128.22, 79.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 52, 1339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3949) [M::mem_pestat] mean and std.dev: (564.71, 1062.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5254) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 251, 1579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4443) [M::mem_pestat] mean and std.dev: (850.03, 997.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5875) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1241.938 CPU sec, 77.615 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (138, 939020, 79, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 109, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (120.30, 81.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.11, 34.96) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 56, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2152) [M::mem_pestat] mean and std.dev: (162.14, 390.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2860) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 317, 973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2467) [M::mem_pestat] mean and std.dev: (544.72, 506.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3214) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1217.394 CPU sec, 53.837 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (132, 939068, 74, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 133, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (125.60, 70.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 46, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (53.53, 49.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 273, 1558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4248) [M::mem_pestat] mean and std.dev: (714.50, 746.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5593) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1246.177 CPU sec, 74.515 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (143, 938922, 90, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 122, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (122.59, 74.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 58, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (72.13, 113.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1140) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 869, 1280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3326) [M::mem_pestat] mean and std.dev: (831.57, 623.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4349) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1219.665 CPU sec, 53.718 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (128, 938615, 72, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 126, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (130.50, 73.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.01, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 60, 941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2749) [M::mem_pestat] mean and std.dev: (175.95, 366.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3653) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 206, 871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2265) [M::mem_pestat] mean and std.dev: (425.35, 492.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2962) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1260.278 CPU sec, 76.139 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (139, 938861, 82, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 127, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (125.28, 81.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 64, 961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2817) [M::mem_pestat] mean and std.dev: (252.50, 471.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3745) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 785, 1241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3285) [M::mem_pestat] mean and std.dev: (758.19, 653.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4307) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1214.098 CPU sec, 53.448 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (146, 938811, 68, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 117, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (125.00, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 50, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 170) [M::mem_pestat] mean and std.dev: (46.09, 27.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 217) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 336, 1093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2901) [M::mem_pestat] mean and std.dev: (624.34, 666.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3805) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1263.568 CPU sec, 74.561 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (129, 938797, 77, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 117, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (122.03, 77.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.10, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 56, 831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2425) [M::mem_pestat] mean and std.dev: (180.92, 421.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3222) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 333, 749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1975) [M::mem_pestat] mean and std.dev: (435.86, 380.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2588) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1233.215 CPU sec, 56.509 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (144, 938952, 71, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 124, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (121.51, 65.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.83) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 43, 81) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 185) [M::mem_pestat] mean and std.dev: (45.02, 29.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 237) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 355, 1245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3343) [M::mem_pestat] mean and std.dev: (759.60, 740.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4392) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1244.737 CPU sec, 76.743 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (142, 938801, 66, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 126, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (121.36, 72.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.10, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 61, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (69.43, 77.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 689) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 284, 1538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4164) [M::mem_pestat] mean and std.dev: (764.36, 973.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5477) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1224.674 CPU sec, 54.196 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (142, 938750, 65, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 123, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (120.31, 71.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 53, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (57.10, 80.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 538, 974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2456) [M::mem_pestat] mean and std.dev: (651.73, 535.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3197) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1243.071 CPU sec, 76.005 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (136, 937883, 79, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 129, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (125.05, 83.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 50, 99) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 235) [M::mem_pestat] mean and std.dev: (53.27, 44.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 303) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 259, 1004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2672) [M::mem_pestat] mean and std.dev: (468.00, 508.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3506) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1219.070 CPU sec, 53.771 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 938247, 78, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 119, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (118.02, 80.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 49, 769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2241) [M::mem_pestat] mean and std.dev: (166.73, 390.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2977) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 334, 1370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3642) [M::mem_pestat] mean and std.dev: (740.79, 729.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4778) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1219.937 CPU sec, 54.035 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (114, 939095, 67, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 122, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (129.99, 77.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 54, 798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2324) [M::mem_pestat] mean and std.dev: (191.64, 453.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3087) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 513, 1166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3116) [M::mem_pestat] mean and std.dev: (650.06, 592.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4091) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1255.057 CPU sec, 74.266 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (147, 938745, 74, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 126, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 374) [M::mem_pestat] mean and std.dev: (120.78, 59.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (347.99, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 46, 85) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 205) [M::mem_pestat] mean and std.dev: (46.84, 39.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 265) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 310, 1100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2886) [M::mem_pestat] mean and std.dev: (671.61, 612.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3779) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1231.501 CPU sec, 55.921 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (124, 938585, 77, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 130, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (124.87, 64.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 46, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (62.80, 79.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1260) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (99, 251, 1042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2928) [M::mem_pestat] mean and std.dev: (507.36, 505.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3871) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1249.997 CPU sec, 75.195 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (118, 937756, 74, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 125, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (122.33, 72.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 58, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (56.08, 42.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 259, 1268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3412) [M::mem_pestat] mean and std.dev: (534.25, 580.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4484) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1208.006 CPU sec, 53.314 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (121, 938197, 82, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 108, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (117.41, 70.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 54, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (55.28, 54.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 319, 1101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2987) [M::mem_pestat] mean and std.dev: (678.32, 698.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3930) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1241.442 CPU sec, 75.126 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (128, 938486, 82, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 134, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (130.50, 75.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (347.97, 34.94) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 48, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (49.94, 39.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 296, 1134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2982) [M::mem_pestat] mean and std.dev: (607.53, 663.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3906) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1218.779 CPU sec, 53.850 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (95, 938421, 59, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 120, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (126.00, 71.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 52, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (56.11, 49.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 292, 764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1904) [M::mem_pestat] mean and std.dev: (552.07, 534.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2688) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1263.015 CPU sec, 74.587 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (136, 938785, 75, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 132, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (131.48, 89.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 647) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 63, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (62.16, 52.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 351, 1464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4020) [M::mem_pestat] mean and std.dev: (484.87, 470.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5298) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1228.876 CPU sec, 55.749 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (108, 938313, 71, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 139, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (138.75, 91.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 684) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.01, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 52, 1971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5855) [M::mem_pestat] mean and std.dev: (831.76, 1597.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7797) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 285, 1178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3122) [M::mem_pestat] mean and std.dev: (697.81, 692.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4094) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1244.355 CPU sec, 75.692 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (126, 937997, 73, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 130, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (130.89, 80.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 62, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (67.00, 63.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 349, 1005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2653) [M::mem_pestat] mean and std.dev: (640.65, 572.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3477) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1216.463 CPU sec, 53.737 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (127, 938411, 80, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 119, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (119.93, 72.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 51, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 189) [M::mem_pestat] mean and std.dev: (42.52, 22.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 242) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 279, 1132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2984) [M::mem_pestat] mean and std.dev: (616.92, 614.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3910) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1230.454 CPU sec, 73.241 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (111, 939193, 69, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 134, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (130.82, 69.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 43, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 159) [M::mem_pestat] mean and std.dev: (39.12, 23.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 202) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 423, 1163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3125) [M::mem_pestat] mean and std.dev: (841.41, 884.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4379) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1229.349 CPU sec, 54.177 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (142, 939126, 72, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 153, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (137.69, 73.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 53, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1160) [M::mem_pestat] mean and std.dev: (93.09, 187.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1536) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 267, 1240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3306) [M::mem_pestat] mean and std.dev: (654.80, 676.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4339) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1219.876 CPU sec, 55.896 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (122, 937370, 62, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 127, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (125.70, 69.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.10, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 47, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 157) [M::mem_pestat] mean and std.dev: (43.69, 22.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 199) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 446, 870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2296) [M::mem_pestat] mean and std.dev: (559.17, 541.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3009) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1261.600 CPU sec, 75.201 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (129, 938772, 76, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 123, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (125.98, 64.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 70, 1409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4149) [M::mem_pestat] mean and std.dev: (571.93, 1061.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5519) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 219, 942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2496) [M::mem_pestat] mean and std.dev: (544.57, 553.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3273) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1223.998 CPU sec, 55.770 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (120, 939122, 68, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 127, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (123.22, 75.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 53, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024) [M::mem_pestat] mean and std.dev: (74.40, 120.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1355) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 318, 1080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2818) [M::mem_pestat] mean and std.dev: (679.12, 700.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3687) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1225.914 CPU sec, 72.820 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (158, 939477, 62, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 114, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (109.68, 63.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 50, 954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2804) [M::mem_pestat] mean and std.dev: (330.91, 683.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3729) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 274, 845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2105) [M::mem_pestat] mean and std.dev: (522.37, 505.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2735) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1212.646 CPU sec, 53.619 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (124, 940055, 52, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 118, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (117.88, 69.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 67, 1332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3912) [M::mem_pestat] mean and std.dev: (525.91, 980.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5202) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (135, 243, 793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2109) [M::mem_pestat] mean and std.dev: (459.89, 486.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2767) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1259.700 CPU sec, 75.213 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (117, 939454, 80, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 116, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (120.90, 72.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 51, 102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248) [M::mem_pestat] mean and std.dev: (52.73, 39.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 321) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 220, 989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2685) [M::mem_pestat] mean and std.dev: (469.73, 539.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3533) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1216.543 CPU sec, 53.683 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 939306, 67, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 124, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 475) [M::mem_pestat] mean and std.dev: (126.52, 81.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 50, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963) [M::mem_pestat] mean and std.dev: (80.30, 141.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1274) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 792, 1169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3093) [M::mem_pestat] mean and std.dev: (685.72, 520.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4055) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1260.993 CPU sec, 75.585 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (139, 938589, 66, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 113, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (112.19, 62.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 60, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (66.11, 63.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 267, 1068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2830) [M::mem_pestat] mean and std.dev: (597.03, 580.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3711) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1232.935 CPU sec, 56.071 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (129, 939534, 75, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 103, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (116.66, 80.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.01, 34.93) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 80, 3417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10159) [M::mem_pestat] mean and std.dev: (1597.11, 2436.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13530) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 424, 1208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3182) [M::mem_pestat] mean and std.dev: (630.11, 524.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4169) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1230.166 CPU sec, 75.075 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (143, 938006, 77, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 125, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 368) [M::mem_pestat] mean and std.dev: (118.83, 65.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 67, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 941) [M::mem_pestat] mean and std.dev: (91.16, 136.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1241) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 294, 1172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3152) [M::mem_pestat] mean and std.dev: (635.00, 591.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4142) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1223.055 CPU sec, 54.234 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (130, 939045, 63, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 130, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (124.77, 70.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 54, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (63.82, 72.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (126, 453, 849) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2295) [M::mem_pestat] mean and std.dev: (530.58, 476.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3018) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1242.805 CPU sec, 73.591 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (122, 938338, 71, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 132, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (125.68, 75.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 50, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (53.41, 64.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 594, 1199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3177) [M::mem_pestat] mean and std.dev: (646.14, 525.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4166) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1220.582 CPU sec, 53.828 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (143, 939090, 61, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 131, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (122.33, 67.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 50, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (48.14, 33.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 357) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 229, 896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2376) [M::mem_pestat] mean and std.dev: (477.85, 477.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3116) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1230.945 CPU sec, 58.999 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (158, 939074, 53, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 107, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (113.01, 68.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 54, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 675) [M::mem_pestat] mean and std.dev: (65.29, 85.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 544, 1103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2877) [M::mem_pestat] mean and std.dev: (538.10, 428.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3764) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1264.773 CPU sec, 75.181 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (127, 938862, 77, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 126, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (129.50, 83.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 62, 1257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3679) [M::mem_pestat] mean and std.dev: (492.00, 906.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4890) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 318, 995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2573) [M::mem_pestat] mean and std.dev: (646.68, 541.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3362) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1217.638 CPU sec, 55.636 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (119, 938499, 66, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 129, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (120.95, 73.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 42, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (47.29, 46.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 339, 689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1697) [M::mem_pestat] mean and std.dev: (457.56, 397.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2201) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1250.047 CPU sec, 75.027 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (116, 938388, 71, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 120, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (126.36, 76.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 53, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (74.58, 99.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 259, 1074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2888) [M::mem_pestat] mean and std.dev: (573.73, 575.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3795) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1218.145 CPU sec, 53.912 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (129, 938807, 84, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 140, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (129.79, 71.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 68, 1559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4615) [M::mem_pestat] mean and std.dev: (702.29, 1233.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6143) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 295, 827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2065) [M::mem_pestat] mean and std.dev: (457.35, 420.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2684) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1251.610 CPU sec, 74.880 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (112, 938524, 78, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 96, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (106.86, 65.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.15, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 64, 976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2860) [M::mem_pestat] mean and std.dev: (206.12, 463.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3802) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 218, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (212.76, 119.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 781) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1218.500 CPU sec, 53.656 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 939178, 85, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 126, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (121.35, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 72, 2044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6054) [M::mem_pestat] mean and std.dev: (847.65, 1364.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8059) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 295, 1125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2917) [M::mem_pestat] mean and std.dev: (632.13, 663.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3813) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1264.054 CPU sec, 75.138 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (144, 938243, 68, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 110, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (119.75, 77.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (347.98, 34.94) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 55, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1127) [M::mem_pestat] mean and std.dev: (77.02, 100.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1492) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 292, 1124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2962) [M::mem_pestat] mean and std.dev: (556.39, 553.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3881) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1226.267 CPU sec, 55.932 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (121, 939100, 76, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 137, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (137.83, 81.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 44, 85) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 199) [M::mem_pestat] mean and std.dev: (45.00, 31.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 256) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 484, 1052) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2694) [M::mem_pestat] mean and std.dev: (672.34, 591.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3515) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1230.575 CPU sec, 75.288 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (132, 938641, 70, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 118, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (117.76, 79.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 44, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (51.65, 57.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 253, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3139) [M::mem_pestat] mean and std.dev: (566.25, 640.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4130) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1219.687 CPU sec, 53.930 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (119, 938420, 65, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 121, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (124.57, 81.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 57, 110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (61.45, 52.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 234, 688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1750) [M::mem_pestat] mean and std.dev: (392.46, 364.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2281) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1261.433 CPU sec, 76.891 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (135, 938178, 79, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 118, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (123.49, 71.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 70, 1204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3530) [M::mem_pestat] mean and std.dev: (259.52, 511.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4693) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 255, 1298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3538) [M::mem_pestat] mean and std.dev: (744.92, 784.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4658) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1230.771 CPU sec, 54.248 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (122, 938954, 76, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 130, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (121.66, 71.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.83) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 51, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (56.38, 58.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 281, 814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2080) [M::mem_pestat] mean and std.dev: (442.03, 423.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2713) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1230.643 CPU sec, 60.386 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (119, 938996, 64, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 136, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (119.10, 68.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 53, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (66.39, 86.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1124) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 871, 2013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5689) [M::mem_pestat] mean and std.dev: (1123.72, 1226.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1240.101 CPU sec, 75.704 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (136, 938398, 62, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 136, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (130.35, 81.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.10, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 61, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (61.47, 53.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 838, 1241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3323) [M::mem_pestat] mean and std.dev: (857.58, 857.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4364) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1224.773 CPU sec, 54.321 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (119, 938513, 75, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 137, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (136.36, 67.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 58, 713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2071) [M::mem_pestat] mean and std.dev: (103.41, 227.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2750) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 216, 1091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2993) [M::mem_pestat] mean and std.dev: (496.25, 517.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3944) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1245.006 CPU sec, 74.532 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (130, 939376, 80, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 139, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (134.46, 77.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 60, 1481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4387) [M::mem_pestat] mean and std.dev: (466.00, 895.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5840) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 259, 874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2176) [M::mem_pestat] mean and std.dev: (472.53, 476.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2827) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1214.569 CPU sec, 53.431 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (147, 938988, 70, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 120, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (119.03, 76.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 65, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1671) [M::mem_pestat] mean and std.dev: (166.81, 306.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2214) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 701, 1174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3084) [M::mem_pestat] mean and std.dev: (708.69, 660.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4039) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1250.936 CPU sec, 76.677 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (139, 938506, 67, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 121, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (118.91, 65.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (347.99, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 65, 2523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7483) [M::mem_pestat] mean and std.dev: (1055.39, 1848.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9963) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 664, 1130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2912) [M::mem_pestat] mean and std.dev: (691.72, 570.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3803) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1212.178 CPU sec, 53.392 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 938974, 62, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 126, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (122.66, 73.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 45, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (42.19, 31.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 436, 1280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3420) [M::mem_pestat] mean and std.dev: (697.75, 618.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4490) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1260.722 CPU sec, 74.309 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (122, 939108, 78, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 116, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (118.54, 71.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 53, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (56.54, 54.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 303, 1645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4495) [M::mem_pestat] mean and std.dev: (813.38, 938.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5920) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1215.260 CPU sec, 55.074 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (121, 938181, 91, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 119, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (119.41, 68.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 55, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (48.91, 31.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (124, 234, 1025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2827) [M::mem_pestat] mean and std.dev: (476.46, 572.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3728) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1248.601 CPU sec, 74.389 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (108, 939083, 80, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 112, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (117.49, 69.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 52, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (55.20, 52.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 577, 926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2426) [M::mem_pestat] mean and std.dev: (619.90, 471.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3176) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1219.950 CPU sec, 53.969 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (145, 937962, 64, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 123, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (126.34, 70.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 47, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 228) [M::mem_pestat] mean and std.dev: (50.81, 38.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 293) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 649, 1306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3474) [M::mem_pestat] mean and std.dev: (816.45, 730.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4558) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1249.426 CPU sec, 76.666 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (132, 939270, 79, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 129, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (131.84, 86.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 60, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (59.89, 51.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 527, 875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2271) [M::mem_pestat] mean and std.dev: (492.90, 375.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2969) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1219.619 CPU sec, 53.870 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 939407, 84, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 120, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (116.31, 71.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.12, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 65, 1056) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3106) [M::mem_pestat] mean and std.dev: (424.32, 862.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4131) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 285, 1136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2962) [M::mem_pestat] mean and std.dev: (739.45, 715.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3875) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1225.667 CPU sec, 61.780 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (119, 938771, 70, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 136, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (121.60, 67.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 55, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 188) [M::mem_pestat] mean and std.dev: (47.48, 29.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 242) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 262, 1046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2782) [M::mem_pestat] mean and std.dev: (465.54, 466.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3650) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1244.208 CPU sec, 73.860 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (137, 939200, 64, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 116, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (123.27, 75.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 59, 108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 256) [M::mem_pestat] mean and std.dev: (50.65, 34.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 330) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 466, 1208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3280) [M::mem_pestat] mean and std.dev: (733.00, 771.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4316) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1215.978 CPU sec, 53.641 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (163, 938648, 75, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 136, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (127.73, 76.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.11, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 46, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 299) [M::mem_pestat] mean and std.dev: (50.72, 42.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 516, 980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2558) [M::mem_pestat] mean and std.dev: (512.59, 410.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3347) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1237.557 CPU sec, 74.443 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (109, 938591, 58, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 117, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (122.76, 79.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.10, 34.82) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 54, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 957) [M::mem_pestat] mean and std.dev: (54.23, 55.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1266) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 729, 1476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4034) [M::mem_pestat] mean and std.dev: (791.68, 760.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5313) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1210.839 CPU sec, 53.260 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (139, 938359, 77, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 139, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (133.23, 71.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 58, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (73.15, 86.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 256, 894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2354) [M::mem_pestat] mean and std.dev: (509.66, 537.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3084) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1250.163 CPU sec, 75.620 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (117, 938137, 73, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 141, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (143.21, 90.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.82) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 57, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1220) [M::mem_pestat] mean and std.dev: (110.08, 202.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1616) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 248, 993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2635) [M::mem_pestat] mean and std.dev: (502.52, 576.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3456) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1214.876 CPU sec, 53.458 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (130, 939618, 84, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 123, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (120.87, 65.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 53, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 535) [M::mem_pestat] mean and std.dev: (51.69, 41.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 264, 786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1988) [M::mem_pestat] mean and std.dev: (464.39, 373.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2589) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1250.719 CPU sec, 74.129 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (128, 938053, 75, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 102, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (110.03, 77.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 49, 85) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 183) [M::mem_pestat] mean and std.dev: (47.20, 23.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 232) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 233, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1021) [M::mem_pestat] mean and std.dev: (225.44, 126.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1299) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1224.234 CPU sec, 56.190 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 939406, 77, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 122, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (120.79, 65.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 51, 561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1615) [M::mem_pestat] mean and std.dev: (148.32, 307.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2142) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (99, 213, 1068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3006) [M::mem_pestat] mean and std.dev: (527.09, 655.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3975) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1236.086 CPU sec, 74.288 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (112, 938613, 66, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 138, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (131.92, 76.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 59, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (60.38, 57.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 637, 1233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3295) [M::mem_pestat] mean and std.dev: (778.55, 756.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4326) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1221.502 CPU sec, 54.055 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 938404, 84, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 131, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (129.50, 68.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 60, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (59.33, 49.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 707, 1318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3486) [M::mem_pestat] mean and std.dev: (764.89, 628.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4570) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1248.388 CPU sec, 77.230 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (116, 938993, 85, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 140, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (126.17, 70.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.10, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 53, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (47.81, 39.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 418, 946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2358) [M::mem_pestat] mean and std.dev: (523.68, 410.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3064) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1215.448 CPU sec, 53.660 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (123, 938278, 81, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 114, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (124.56, 79.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 49, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 947) [M::mem_pestat] mean and std.dev: (90.85, 144.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1251) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 236, 397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (260.30, 165.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1090) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1242.229 CPU sec, 64.510 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (128, 938482, 79, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 130, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (126.26, 75.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 60, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1249) [M::mem_pestat] mean and std.dev: (101.38, 183.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1653) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 316, 1258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3388) [M::mem_pestat] mean and std.dev: (709.39, 752.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4453) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1233.888 CPU sec, 68.203 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (102, 939408, 95, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 112, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (111.59, 66.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 62, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1145) [M::mem_pestat] mean and std.dev: (87.46, 154.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1515) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 242, 1156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3154) [M::mem_pestat] mean and std.dev: (620.93, 702.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4153) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1217.189 CPU sec, 53.549 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (131, 938749, 61, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (50, 99, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (107.03, 65.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 44, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 151) [M::mem_pestat] mean and std.dev: (40.24, 21.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 192) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 374, 920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2420) [M::mem_pestat] mean and std.dev: (475.00, 407.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3170) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1239.377 CPU sec, 73.807 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (134, 939062, 68, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 107, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (114.37, 73.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 66, 2659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7901) [M::mem_pestat] mean and std.dev: (932.94, 1859.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10522) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 250, 1190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3222) [M::mem_pestat] mean and std.dev: (393.57, 449.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4238) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1221.088 CPU sec, 53.720 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (135, 939573, 75, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 107, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (116.50, 73.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 58, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (51.47, 31.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 761, 1432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3810) [M::mem_pestat] mean and std.dev: (841.80, 723.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4999) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1251.669 CPU sec, 75.801 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (126, 938960, 84, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 110, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (114.97, 75.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 52, 1234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3634) [M::mem_pestat] mean and std.dev: (511.09, 971.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4834) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 233, 939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2457) [M::mem_pestat] mean and std.dev: (496.26, 544.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3216) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1217.462 CPU sec, 53.690 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (129, 938570, 78, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 122, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (123.89, 75.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 59, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1780) [M::mem_pestat] mean and std.dev: (169.05, 338.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2361) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 751, 1558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4326) [M::mem_pestat] mean and std.dev: (787.96, 844.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5710) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1265.558 CPU sec, 74.953 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (127, 938142, 73, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 110, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 509) [M::mem_pestat] mean and std.dev: (115.81, 78.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 61, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2577) [M::mem_pestat] mean and std.dev: (252.45, 530.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3425) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 293, 1207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3287) [M::mem_pestat] mean and std.dev: (639.82, 685.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4327) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1227.725 CPU sec, 55.902 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (148, 938930, 70, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 121, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (122.42, 67.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 45, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (42.30, 31.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 634) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 346, 1086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2930) [M::mem_pestat] mean and std.dev: (656.74, 641.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3852) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1238.382 CPU sec, 73.668 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (125, 938460, 79, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 114, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (115.81, 69.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 60, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (72.23, 80.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 640, 1414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3874) [M::mem_pestat] mean and std.dev: (883.14, 933.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5104) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1207.717 CPU sec, 53.422 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (152, 938824, 58, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 129, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (135.16, 92.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 64, 357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989) [M::mem_pestat] mean and std.dev: (107.79, 155.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1305) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 918, 2099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5851) [M::mem_pestat] mean and std.dev: (1093.54, 1048.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7727) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1248.693 CPU sec, 74.171 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (122, 938762, 76, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 107, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (112.12, 63.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 66, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1280) [M::mem_pestat] mean and std.dev: (116.97, 190.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1693) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 645, 1006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2584) [M::mem_pestat] mean and std.dev: (620.10, 465.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3373) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1210.861 CPU sec, 53.274 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (131, 938877, 79, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 115, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (111.10, 66.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 52, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (84.65, 140.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 262, 1035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2691) [M::mem_pestat] mean and std.dev: (623.04, 581.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3519) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1230.246 CPU sec, 64.765 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (138, 939115, 70, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 129, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (119.87, 71.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 44, 85) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 209) [M::mem_pestat] mean and std.dev: (39.13, 23.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 271) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 265, 836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2198) [M::mem_pestat] mean and std.dev: (494.61, 457.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2879) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1243.004 CPU sec, 73.351 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (127, 938885, 76, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 120, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (123.29, 67.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 59, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1416) [M::mem_pestat] mean and std.dev: (117.11, 232.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1876) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 763, 1681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4561) [M::mem_pestat] mean and std.dev: (1018.77, 971.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6001) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1204.125 CPU sec, 53.025 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (127, 939097, 62, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 125, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (127.29, 83.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 58, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 253) [M::mem_pestat] mean and std.dev: (45.66, 22.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 326) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 299, 1827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5091) [M::mem_pestat] mean and std.dev: (876.83, 1085.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6723) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1234.113 CPU sec, 72.700 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 938312, 72, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 122, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (123.93, 72.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 50, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 644) [M::mem_pestat] mean and std.dev: (55.11, 58.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 849) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 659, 1440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3920) [M::mem_pestat] mean and std.dev: (770.65, 731.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5160) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1201.494 CPU sec, 52.860 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 938269, 63, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 133, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (129.92, 85.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 54, 782) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2274) [M::mem_pestat] mean and std.dev: (220.22, 433.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3020) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 347, 835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2151) [M::mem_pestat] mean and std.dev: (458.09, 434.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2809) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1246.425 CPU sec, 75.222 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 938475, 89, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 118, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (126.18, 80.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (347.97, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 47, 1508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4464) [M::mem_pestat] mean and std.dev: (428.11, 1072.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5942) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 683, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3075) [M::mem_pestat] mean and std.dev: (720.47, 719.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4034) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1215.570 CPU sec, 53.511 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (138, 939077, 79, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 109, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (117.99, 77.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 52, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 209) [M::mem_pestat] mean and std.dev: (47.54, 28.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 268) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 277, 1267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3421) [M::mem_pestat] mean and std.dev: (700.96, 701.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4498) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1199.336 CPU sec, 52.897 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (117, 939268, 77, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 113, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (121.48, 73.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 65, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075) [M::mem_pestat] mean and std.dev: (104.06, 150.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1422) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 278, 834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2074) [M::mem_pestat] mean and std.dev: (460.25, 434.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2694) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1243.592 CPU sec, 75.183 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 938334, 86, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 120, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (121.96, 72.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 52, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 672) [M::mem_pestat] mean and std.dev: (74.88, 103.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 578, 985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2595) [M::mem_pestat] mean and std.dev: (607.44, 523.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3400) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1206.800 CPU sec, 53.197 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (125, 938987, 88, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 130, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (134.01, 78.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 60, 1187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3491) [M::mem_pestat] mean and std.dev: (479.65, 891.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4643) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 379, 819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2143) [M::mem_pestat] mean and std.dev: (483.46, 436.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2805) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1225.953 CPU sec, 72.955 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (125, 938088, 87, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 126, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (130.29, 88.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.01, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 61, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (83.97, 129.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 257, 957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2531) [M::mem_pestat] mean and std.dev: (566.28, 568.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3318) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1199.124 CPU sec, 52.755 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (129, 939435, 66, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 119, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (119.90, 68.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 60, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 597) [M::mem_pestat] mean and std.dev: (72.87, 92.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 786) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 259, 1042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2746) [M::mem_pestat] mean and std.dev: (590.67, 545.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3598) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1227.441 CPU sec, 74.483 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (130, 938656, 70, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 116, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (114.30, 71.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 59, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (63.22, 70.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 279, 851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2233) [M::mem_pestat] mean and std.dev: (456.68, 399.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2924) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1203.020 CPU sec, 52.895 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 938277, 73, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 132, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (126.98, 71.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 56, 1150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3388) [M::mem_pestat] mean and std.dev: (370.48, 818.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4507) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 317, 803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2007) [M::mem_pestat] mean and std.dev: (471.39, 415.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2609) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1249.369 CPU sec, 74.749 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (142, 938562, 82, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 116, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (122.71, 77.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 48, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (52.30, 50.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 380) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 256, 789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2071) [M::mem_pestat] mean and std.dev: (525.17, 567.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2794) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1221.327 CPU sec, 55.904 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (147, 939268, 86, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 125, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (127.88, 78.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.82) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 53, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1131) [M::mem_pestat] mean and std.dev: (79.21, 122.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1495) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 710, 1099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2819) [M::mem_pestat] mean and std.dev: (780.82, 635.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3679) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1236.042 CPU sec, 73.195 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (145, 937511, 75, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 118, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (118.61, 69.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 65, 1407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4133) [M::mem_pestat] mean and std.dev: (356.30, 753.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5496) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 254, 793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1983) [M::mem_pestat] mean and std.dev: (533.82, 479.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2578) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1211.609 CPU sec, 53.612 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (131, 938545, 67, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 124, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (120.79, 65.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.01, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 47, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (51.08, 51.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 318, 743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1801) [M::mem_pestat] mean and std.dev: (425.31, 327.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2330) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1240.987 CPU sec, 74.146 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (141, 938245, 76, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 114, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (124.32, 75.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 56, 1106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3264) [M::mem_pestat] mean and std.dev: (375.55, 692.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4343) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 249, 783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2015) [M::mem_pestat] mean and std.dev: (452.80, 480.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2631) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1221.192 CPU sec, 53.844 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (132, 939098, 72, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 98, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (103.26, 63.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.95) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 65, 715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2087) [M::mem_pestat] mean and std.dev: (175.26, 362.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2773) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (134, 243, 1129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3119) [M::mem_pestat] mean and std.dev: (591.11, 668.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4114) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1210.404 CPU sec, 53.359 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (153, 938676, 72, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 135, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (132.09, 72.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 63, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (60.95, 56.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 840) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 275, 1120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2966) [M::mem_pestat] mean and std.dev: (660.82, 642.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3889) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1241.845 CPU sec, 74.077 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (119, 938839, 65, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 122, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (113.86, 63.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.01, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 57, 1823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5395) [M::mem_pestat] mean and std.dev: (795.05, 1393.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7181) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 591, 1303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3515) [M::mem_pestat] mean and std.dev: (678.00, 607.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4621) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1219.633 CPU sec, 56.004 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 938738, 78, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 115, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (119.61, 70.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 64, 775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2265) [M::mem_pestat] mean and std.dev: (241.17, 487.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3010) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 265, 872) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2298) [M::mem_pestat] mean and std.dev: (450.04, 482.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3011) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1237.611 CPU sec, 73.683 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (124, 939127, 67, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 116, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (115.89, 69.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 50, 89) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 215) [M::mem_pestat] mean and std.dev: (44.76, 30.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 278) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 255, 909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2333) [M::mem_pestat] mean and std.dev: (482.00, 422.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3045) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1210.594 CPU sec, 53.715 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 938822, 81, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 138, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (130.80, 71.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 56, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (67.65, 68.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 951, 1479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3981) [M::mem_pestat] mean and std.dev: (1020.03, 942.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5232) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1248.744 CPU sec, 75.967 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (142, 939213, 88, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 122, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 526) [M::mem_pestat] mean and std.dev: (127.89, 83.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 55, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (70.42, 86.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 576, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2997) [M::mem_pestat] mean and std.dev: (663.95, 647.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3917) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1220.103 CPU sec, 53.885 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (147, 938218, 92, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 129, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (123.42, 66.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 68, 231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (68.19, 57.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 801) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 237, 682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1744) [M::mem_pestat] mean and std.dev: (364.31, 332.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2275) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1254.954 CPU sec, 74.080 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (128, 938690, 67, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 124, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (121.70, 64.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 63, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2442) [M::mem_pestat] mean and std.dev: (286.93, 517.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3244) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 213, 695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1795) [M::mem_pestat] mean and std.dev: (341.56, 325.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2345) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1211.457 CPU sec, 55.381 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (120, 938193, 68, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 125, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (128.65, 75.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (347.99, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 47, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 201) [M::mem_pestat] mean and std.dev: (43.05, 29.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 260) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 229, 649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1605) [M::mem_pestat] mean and std.dev: (361.85, 341.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2083) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1238.648 CPU sec, 75.948 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (136, 938873, 69, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 124, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (121.69, 71.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.10, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 42, 92) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 230) [M::mem_pestat] mean and std.dev: (46.65, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 299) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 646, 1378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3720) [M::mem_pestat] mean and std.dev: (860.44, 822.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4891) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1205.779 CPU sec, 53.458 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (135, 938487, 87, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 132, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (127.07, 79.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 44, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (53.66, 79.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 324, 1054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2790) [M::mem_pestat] mean and std.dev: (640.24, 588.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3658) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1239.856 CPU sec, 73.935 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (138, 939093, 79, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 114, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (111.03, 70.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.10, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 64, 870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2526) [M::mem_pestat] mean and std.dev: (330.43, 637.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3354) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 293, 1133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3057) [M::mem_pestat] mean and std.dev: (616.05, 643.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4019) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1214.408 CPU sec, 53.556 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (150, 938690, 70, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 126, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (122.51, 65.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.16, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 85, 807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2355) [M::mem_pestat] mean and std.dev: (218.84, 451.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3129) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 531, 1170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3066) [M::mem_pestat] mean and std.dev: (722.82, 660.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4014) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1219.407 CPU sec, 55.809 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (121, 938992, 74, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 127, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (121.89, 61.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.11, 34.82) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 53, 807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2361) [M::mem_pestat] mean and std.dev: (200.56, 453.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3138) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 588, 1595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4417) [M::mem_pestat] mean and std.dev: (819.06, 796.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5828) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1244.510 CPU sec, 75.925 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (123, 937905, 65, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 129, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (127.58, 67.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.15, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 58, 1387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4109) [M::mem_pestat] mean and std.dev: (331.56, 841.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5470) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 299, 956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2480) [M::mem_pestat] mean and std.dev: (578.21, 518.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3242) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1214.916 CPU sec, 54.207 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (138, 938365, 93, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 132, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (125.41, 74.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 59, 819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2385) [M::mem_pestat] mean and std.dev: (183.20, 397.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3168) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 344, 1247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3337) [M::mem_pestat] mean and std.dev: (678.21, 606.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4382) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1255.457 CPU sec, 75.651 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (152, 938754, 87, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 126, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (122.74, 70.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 63, 1434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4232) [M::mem_pestat] mean and std.dev: (440.87, 877.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5631) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 261, 1460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4080) [M::mem_pestat] mean and std.dev: (741.88, 774.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5390) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1201.424 CPU sec, 53.048 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (113, 939675, 70, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 109, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (115.38, 70.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.83) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 49, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (52.71, 50.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 271, 849) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2175) [M::mem_pestat] mean and std.dev: (432.33, 418.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2838) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1246.116 CPU sec, 74.760 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 938717, 78, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 117, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (119.31, 65.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 54, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 252) [M::mem_pestat] mean and std.dev: (49.57, 31.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 325) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 318, 936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2424) [M::mem_pestat] mean and std.dev: (579.16, 588.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3168) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1208.493 CPU sec, 53.179 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (122, 940138, 80, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 110, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (117.55, 72.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (347.99, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 62, 1760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5224) [M::mem_pestat] mean and std.dev: (796.53, 1323.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6956) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (126, 291, 1761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5031) [M::mem_pestat] mean and std.dev: (769.62, 882.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6666) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1263.762 CPU sec, 74.776 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (157, 939048, 67, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 105, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (114.76, 64.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.10, 34.94) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 49, 109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 267) [M::mem_pestat] mean and std.dev: (49.56, 36.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 317, 1343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3615) [M::mem_pestat] mean and std.dev: (772.28, 823.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4751) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1216.999 CPU sec, 55.605 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (130, 938417, 67, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 112, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (111.88, 74.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.01, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 46, 926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2710) [M::mem_pestat] mean and std.dev: (285.30, 632.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3602) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 411, 1354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3696) [M::mem_pestat] mean and std.dev: (673.48, 633.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4867) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1251.044 CPU sec, 74.241 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (130, 939481, 60, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 129, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (122.40, 67.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 52, 958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2812) [M::mem_pestat] mean and std.dev: (281.53, 548.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3739) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 246, 902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2348) [M::mem_pestat] mean and std.dev: (476.50, 472.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3071) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1197.733 CPU sec, 52.938 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (147, 939502, 82, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 114, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (122.31, 73.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 62, 699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2033) [M::mem_pestat] mean and std.dev: (245.60, 436.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2700) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 229, 627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1487) [M::mem_pestat] mean and std.dev: (346.16, 289.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1917) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1233.074 CPU sec, 71.614 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (135, 939232, 75, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 121, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (116.17, 63.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 62, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (77.05, 83.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 328, 1218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3270) [M::mem_pestat] mean and std.dev: (615.72, 599.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4296) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1215.968 CPU sec, 53.703 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (120, 938135, 74, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 116, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (117.84, 71.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 53, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 921) [M::mem_pestat] mean and std.dev: (105.60, 197.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1219) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 344, 1174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3170) [M::mem_pestat] mean and std.dev: (686.19, 687.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4168) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1206.699 CPU sec, 57.208 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (110, 938776, 68, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 108, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (120.97, 87.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 65, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029) [M::mem_pestat] mean and std.dev: (107.07, 168.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1359) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (264, 809, 1360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3552) [M::mem_pestat] mean and std.dev: (805.39, 631.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4648) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1253.748 CPU sec, 76.122 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 938711, 70, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 139, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (131.42, 76.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.12, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 53, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2450) [M::mem_pestat] mean and std.dev: (235.17, 472.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3256) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (108, 178, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 981) [M::mem_pestat] mean and std.dev: (156.06, 93.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1272) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1221.513 CPU sec, 54.878 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (134, 938915, 98, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 116, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (122.82, 73.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 55, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 202) [M::mem_pestat] mean and std.dev: (44.96, 29.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 261) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 248, 1553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4371) [M::mem_pestat] mean and std.dev: (925.60, 1243.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5900) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1239.381 CPU sec, 73.914 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (151, 938703, 95, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 117, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (120.29, 65.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 56, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (63.96, 82.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (124, 215, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1078) [M::mem_pestat] mean and std.dev: (237.18, 217.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1396) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1216.794 CPU sec, 53.641 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (153, 938916, 81, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 120, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (117.75, 73.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 65, 790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2288) [M::mem_pestat] mean and std.dev: (177.42, 384.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3037) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 364, 1004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2624) [M::mem_pestat] mean and std.dev: (609.36, 614.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3434) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1220.764 CPU sec, 70.650 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (130, 938572, 45, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 111, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (117.42, 72.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 58, 776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2254) [M::mem_pestat] mean and std.dev: (200.45, 410.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2993) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 245, 993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2623) [M::mem_pestat] mean and std.dev: (362.09, 322.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3438) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1210.123 CPU sec, 53.322 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (135, 939260, 89, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 109, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (116.30, 78.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 55, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (56.57, 54.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 297, 808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2094) [M::mem_pestat] mean and std.dev: (488.86, 494.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2737) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1274.411 CPU sec, 74.917 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 938455, 76, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 126, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (121.77, 80.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.10, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 52, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (48.41, 38.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 470) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 320, 1070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2798) [M::mem_pestat] mean and std.dev: (748.65, 787.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3897) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1210.891 CPU sec, 55.128 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (143, 938434, 86, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 128, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (122.41, 73.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 54, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1640) [M::mem_pestat] mean and std.dev: (115.12, 237.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2175) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 294, 1060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2730) [M::mem_pestat] mean and std.dev: (497.34, 424.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3565) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1250.182 CPU sec, 74.852 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (142, 938582, 82, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 124, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (119.89, 77.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 58, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (59.37, 48.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 288, 1269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3431) [M::mem_pestat] mean and std.dev: (688.67, 744.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4512) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1225.486 CPU sec, 54.244 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (136, 938451, 63, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 114, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (116.86, 72.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 73, 2100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6224) [M::mem_pestat] mean and std.dev: (996.72, 1507.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8286) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 217, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1223) [M::mem_pestat] mean and std.dev: (300.45, 240.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1575) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1226.442 CPU sec, 75.153 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (145, 939133, 77, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 129, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (126.34, 67.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 56, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (56.79, 61.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 284, 1048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2764) [M::mem_pestat] mean and std.dev: (492.28, 514.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3622) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1222.655 CPU sec, 53.956 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 938330, 70, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 112, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (119.26, 72.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 72, 2306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6858) [M::mem_pestat] mean and std.dev: (903.73, 1571.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9134) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 541, 1519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4069) [M::mem_pestat] mean and std.dev: (831.27, 912.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5344) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1224.628 CPU sec, 59.900 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (137, 937515, 55, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 136, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (127.59, 69.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.81) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 63, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (74.00, 93.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 291, 973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2497) [M::mem_pestat] mean and std.dev: (584.36, 502.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3259) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1269.531 CPU sec, 76.014 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 938759, 80, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 120, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (126.59, 69.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 57, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (61.92, 64.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 462) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 246, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3053) [M::mem_pestat] mean and std.dev: (530.18, 593.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4023) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1209.647 CPU sec, 54.044 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (142, 938290, 65, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 115, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (112.28, 69.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 49, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (45.96, 33.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 373) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 276, 1149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3087) [M::mem_pestat] mean and std.dev: (643.83, 807.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4056) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1221.327 CPU sec, 72.262 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (143, 938158, 92, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 117, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 495) [M::mem_pestat] mean and std.dev: (116.87, 78.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 49, 404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1152) [M::mem_pestat] mean and std.dev: (91.56, 171.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1526) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 263, 1289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3425) [M::mem_pestat] mean and std.dev: (682.13, 779.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4493) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1218.326 CPU sec, 53.827 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (130, 938142, 84, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (46, 108, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (101.77, 63.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 63, 1059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3097) [M::mem_pestat] mean and std.dev: (388.84, 783.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4116) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 348, 992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2628) [M::mem_pestat] mean and std.dev: (608.31, 527.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3446) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1226.943 CPU sec, 73.961 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (118, 938101, 75, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 106, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (113.34, 67.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 56, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 696) [M::mem_pestat] mean and std.dev: (76.31, 111.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 916) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 214, 1537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4309) [M::mem_pestat] mean and std.dev: (854.14, 1082.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5695) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1203.832 CPU sec, 52.992 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (148, 939155, 67, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 123, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (131.62, 83.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 62, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2825) [M::mem_pestat] mean and std.dev: (275.71, 585.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3755) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 417, 847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2195) [M::mem_pestat] mean and std.dev: (552.00, 539.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1264.135 CPU sec, 74.689 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (142, 939375, 80, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 125, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (120.98, 72.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.11, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 51, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (47.86, 36.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 262, 920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2382) [M::mem_pestat] mean and std.dev: (514.62, 501.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3113) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1221.994 CPU sec, 55.685 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (126, 938781, 72, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 118, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (119.29, 72.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.14, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 61, 724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2090) [M::mem_pestat] mean and std.dev: (191.03, 371.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2773) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 258, 1349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3739) [M::mem_pestat] mean and std.dev: (660.10, 681.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4934) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1236.779 CPU sec, 71.635 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (125, 938314, 75, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 115, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (126.09, 74.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 60, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 521) [M::mem_pestat] mean and std.dev: (57.47, 42.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 682) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 240, 1287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3491) [M::mem_pestat] mean and std.dev: (615.49, 696.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4593) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1205.014 CPU sec, 53.390 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 938824, 68, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 113, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (114.78, 66.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 58, 828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2408) [M::mem_pestat] mean and std.dev: (222.65, 512.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3198) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 258, 1033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2739) [M::mem_pestat] mean and std.dev: (670.57, 691.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3592) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1242.441 CPU sec, 74.073 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (113, 938593, 56, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 115, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (119.38, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 68, 1984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5872) [M::mem_pestat] mean and std.dev: (1050.91, 1734.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7990) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 266, 699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1685) [M::mem_pestat] mean and std.dev: (400.59, 372.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2178) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1219.056 CPU sec, 53.889 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (139, 939118, 81, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 117, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (118.12, 80.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.11, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 51, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 254) [M::mem_pestat] mean and std.dev: (42.73, 23.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 328) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 286, 808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2006) [M::mem_pestat] mean and std.dev: (527.94, 485.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2605) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1215.176 CPU sec, 61.280 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (131, 938011, 79, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 134, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (129.30, 70.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.11, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 51, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (63.40, 77.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 852) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 304, 999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2641) [M::mem_pestat] mean and std.dev: (610.81, 571.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3462) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1266.440 CPU sec, 76.979 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (145, 938755, 63, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 124, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (122.95, 83.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 69, 674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1954) [M::mem_pestat] mean and std.dev: (209.88, 406.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2594) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 348, 1290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3590) [M::mem_pestat] mean and std.dev: (641.97, 748.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4740) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1221.054 CPU sec, 53.893 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (134, 938351, 69, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 132, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (126.60, 73.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 62, 2195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6527) [M::mem_pestat] mean and std.dev: (854.93, 1455.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8693) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 228, 762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1992) [M::mem_pestat] mean and std.dev: (351.50, 411.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2607) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1248.561 CPU sec, 73.631 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 939410, 64, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 115, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (112.42, 63.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 57, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (64.13, 76.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 698) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 231, 797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2085) [M::mem_pestat] mean and std.dev: (366.81, 340.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2729) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1206.101 CPU sec, 53.064 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (141, 939086, 76, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 136, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (127.07, 75.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 49, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 933) [M::mem_pestat] mean and std.dev: (68.12, 120.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1235) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 228, 973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2639) [M::mem_pestat] mean and std.dev: (509.29, 604.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3472) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1231.559 CPU sec, 72.641 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (139, 937555, 73, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 122, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (116.73, 75.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (347.96, 34.81) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 51, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (57.55, 47.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 420) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (301, 817, 1949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5245) [M::mem_pestat] mean and std.dev: (1062.86, 989.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6893) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1218.791 CPU sec, 53.724 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 938557, 87, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 119, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (118.15, 69.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 48, 112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 270) [M::mem_pestat] mean and std.dev: (49.26, 36.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 349) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 491, 919) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2387) [M::mem_pestat] mean and std.dev: (599.85, 508.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3121) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1238.575 CPU sec, 73.855 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (127, 938831, 75, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (81, 141, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (129.28, 69.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 48, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (70.27, 109.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1190) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 285, 969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2507) [M::mem_pestat] mean and std.dev: (570.15, 560.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3276) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1234.483 CPU sec, 56.472 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (145, 937968, 71, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 133, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (125.37, 69.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 59, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (54.07, 40.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 233, 928) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2444) [M::mem_pestat] mean and std.dev: (524.94, 573.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3202) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1232.031 CPU sec, 72.982 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (125, 939040, 62, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 113, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (120.43, 70.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 51, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 613) [M::mem_pestat] mean and std.dev: (64.88, 80.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 585, 1538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4220) [M::mem_pestat] mean and std.dev: (880.89, 938.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5561) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1206.974 CPU sec, 53.403 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (121, 938667, 74, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 120, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (122.65, 71.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (347.99, 34.96) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 64, 634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1836) [M::mem_pestat] mean and std.dev: (134.88, 288.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2437) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 248, 1652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4582) [M::mem_pestat] mean and std.dev: (966.86, 1281.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6094) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1245.771 CPU sec, 75.399 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (125, 939305, 86, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 107, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (108.30, 66.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.11, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 63, 698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2014) [M::mem_pestat] mean and std.dev: (189.21, 402.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2672) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 266, 817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2015) [M::mem_pestat] mean and std.dev: (415.68, 410.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2614) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1219.018 CPU sec, 53.697 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (147, 938567, 78, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 125, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (124.02, 69.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.01, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 49, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 253) [M::mem_pestat] mean and std.dev: (47.15, 34.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 252, 1043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2775) [M::mem_pestat] mean and std.dev: (565.43, 514.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3641) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1213.326 CPU sec, 63.156 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 938369, 69, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 117, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 490) [M::mem_pestat] mean and std.dev: (118.97, 79.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 635) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.10, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 52, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 667) [M::mem_pestat] mean and std.dev: (74.55, 85.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 879) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 586, 1156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3072) [M::mem_pestat] mean and std.dev: (668.58, 617.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4030) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1227.716 CPU sec, 74.162 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (148, 939167, 74, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 118, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (113.13, 71.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 58, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 262) [M::mem_pestat] mean and std.dev: (54.97, 50.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (98, 252, 805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2219) [M::mem_pestat] mean and std.dev: (438.88, 493.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2926) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1214.819 CPU sec, 53.548 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (134, 938419, 60, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 120, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (127.01, 78.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 74, 938) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2740) [M::mem_pestat] mean and std.dev: (289.31, 567.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3641) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 333, 1301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3451) [M::mem_pestat] mean and std.dev: (822.62, 951.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4628) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1229.346 CPU sec, 74.148 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (139, 938199, 78, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 113, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (114.44, 68.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 62, 834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2434) [M::mem_pestat] mean and std.dev: (278.56, 535.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3234) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 250, 1417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3859) [M::mem_pestat] mean and std.dev: (701.37, 819.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5080) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1207.969 CPU sec, 53.230 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (131, 938119, 74, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 127, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (123.90, 68.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 54, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (70.42, 88.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 679) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 287, 867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2169) [M::mem_pestat] mean and std.dev: (497.03, 399.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2820) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1250.287 CPU sec, 75.559 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (158, 938651, 79, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 132, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (128.34, 69.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.93) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 69, 1251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3681) [M::mem_pestat] mean and std.dev: (440.77, 889.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4896) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 256, 1071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2921) [M::mem_pestat] mean and std.dev: (499.53, 563.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3846) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1210.144 CPU sec, 53.326 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (148, 938871, 86, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 108, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (116.91, 70.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 50, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (66.93, 87.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 706) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 448, 999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2595) [M::mem_pestat] mean and std.dev: (608.67, 509.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3393) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1270.678 CPU sec, 74.629 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (147, 938571, 74, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 115, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (117.10, 78.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 49, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (77.80, 113.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1088) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 287, 1272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3442) [M::mem_pestat] mean and std.dev: (683.45, 748.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1225.730 CPU sec, 55.677 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (139, 937887, 59, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 119, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (121.55, 70.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 53, 116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 272) [M::mem_pestat] mean and std.dev: (55.33, 35.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 338, 1128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3084) [M::mem_pestat] mean and std.dev: (654.74, 655.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4062) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1231.237 CPU sec, 75.244 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (127, 938812, 82, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 111, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (118.48, 75.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.94) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 65, 920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2686) [M::mem_pestat] mean and std.dev: (226.12, 468.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3569) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 395, 1913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5403) [M::mem_pestat] mean and std.dev: (1072.67, 1262.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7148) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1205.267 CPU sec, 53.244 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (137, 938249, 69, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 104, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (114.52, 74.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 58, 416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1186) [M::mem_pestat] mean and std.dev: (112.81, 223.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1571) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (135, 188, 948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2574) [M::mem_pestat] mean and std.dev: (348.32, 442.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3387) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1240.494 CPU sec, 74.841 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (132, 938403, 72, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 128, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (122.41, 64.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 50, 1573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4663) [M::mem_pestat] mean and std.dev: (744.15, 1390.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6305) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 234, 1453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3975) [M::mem_pestat] mean and std.dev: (649.68, 741.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5236) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1212.317 CPU sec, 53.342 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (138, 938469, 86, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 120, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (110.97, 61.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 58, 1010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2972) [M::mem_pestat] mean and std.dev: (262.65, 612.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3953) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 443, 1334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3682) [M::mem_pestat] mean and std.dev: (688.71, 743.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4856) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1228.877 CPU sec, 65.029 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (123, 938977, 58, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 150, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 439) [M::mem_pestat] mean and std.dev: (126.38, 70.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 56, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (56.32, 50.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 259, 995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2585) [M::mem_pestat] mean and std.dev: (599.37, 596.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3380) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1243.524 CPU sec, 69.228 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (135, 939199, 77, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 122, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (128.80, 73.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.95) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 48, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 200) [M::mem_pestat] mean and std.dev: (42.81, 30.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 258) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 404, 993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2525) [M::mem_pestat] mean and std.dev: (569.68, 510.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3291) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1216.245 CPU sec, 53.531 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 938296, 80, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 100, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (112.53, 73.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 58, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (51.22, 31.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 407) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 268, 1015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2629) [M::mem_pestat] mean and std.dev: (521.40, 457.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3436) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1233.723 CPU sec, 75.084 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (130, 938694, 75, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 118, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (115.27, 74.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 60, 869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2531) [M::mem_pestat] mean and std.dev: (197.40, 400.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3362) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 232, 877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2345) [M::mem_pestat] mean and std.dev: (445.88, 550.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3079) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1209.447 CPU sec, 53.224 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (129, 937432, 83, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 120, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (125.31, 89.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 45, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 778) [M::mem_pestat] mean and std.dev: (52.55, 72.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 264, 879) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2243) [M::mem_pestat] mean and std.dev: (502.43, 438.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2925) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1239.682 CPU sec, 76.269 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (113, 938486, 73, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 125, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (120.67, 74.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 60, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (79.38, 105.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1246) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (134, 241, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1274) [M::mem_pestat] mean and std.dev: (316.00, 281.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1654) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1212.332 CPU sec, 53.466 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (160, 939692, 79, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 137, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 513) [M::mem_pestat] mean and std.dev: (126.26, 77.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 51, 786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2290) [M::mem_pestat] mean and std.dev: (141.78, 363.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3042) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (130, 234, 731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1933) [M::mem_pestat] mean and std.dev: (364.00, 350.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2534) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1250.444 CPU sec, 74.036 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (120, 938639, 77, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 136, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (129.27, 74.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 55, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (52.95, 36.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 265, 784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1966) [M::mem_pestat] mean and std.dev: (360.53, 273.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2557) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1212.360 CPU sec, 55.006 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (113, 938155, 75, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 118, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (119.99, 69.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 53, 263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (74.97, 115.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 475, 958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2474) [M::mem_pestat] mean and std.dev: (541.54, 476.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3232) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1234.178 CPU sec, 74.386 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (126, 938618, 58, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 118, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (112.97, 67.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.95) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 65, 561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1603) [M::mem_pestat] mean and std.dev: (150.56, 248.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2124) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 758, 1638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4418) [M::mem_pestat] mean and std.dev: (797.42, 690.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5808) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1215.843 CPU sec, 53.773 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (118, 939802, 74, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 119, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (116.95, 67.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 57, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 589) [M::mem_pestat] mean and std.dev: (60.00, 53.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 774) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 325, 1514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4100) [M::mem_pestat] mean and std.dev: (650.96, 612.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5393) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1248.120 CPU sec, 74.287 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (129, 937368, 72, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 125, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (125.10, 70.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 66, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2262) [M::mem_pestat] mean and std.dev: (210.36, 439.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3003) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 503, 1535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4155) [M::mem_pestat] mean and std.dev: (872.97, 900.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5465) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1216.849 CPU sec, 53.695 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (135, 939283, 80, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 131, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (129.99, 77.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 64, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1786) [M::mem_pestat] mean and std.dev: (93.06, 163.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2371) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 257, 1007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2689) [M::mem_pestat] mean and std.dev: (501.12, 473.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3530) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1216.607 CPU sec, 66.514 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (139, 938507, 92, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 126, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (126.36, 69.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 44, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 188) [M::mem_pestat] mean and std.dev: (42.16, 26.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 242) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 271, 587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1443) [M::mem_pestat] mean and std.dev: (374.19, 306.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1871) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1257.099 CPU sec, 75.839 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (116, 938883, 68, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 125, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (128.29, 81.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.12, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 51, 88) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 190) [M::mem_pestat] mean and std.dev: (52.43, 29.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 241) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 261, 1108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2990) [M::mem_pestat] mean and std.dev: (593.90, 533.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3931) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1209.414 CPU sec, 53.324 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (122, 938866, 87, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 121, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (115.33, 69.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 55, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (75.62, 93.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 962) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 257, 933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2541) [M::mem_pestat] mean and std.dev: (517.08, 539.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3345) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1246.241 CPU sec, 75.233 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (141, 938600, 71, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 134, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (119.56, 74.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 53, 87) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 207) [M::mem_pestat] mean and std.dev: (46.14, 27.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 267) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 336, 1193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3199) [M::mem_pestat] mean and std.dev: (632.45, 587.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4202) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1207.912 CPU sec, 53.200 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (108, 940015, 77, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 120, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (120.99, 73.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 62, 815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2375) [M::mem_pestat] mean and std.dev: (124.33, 257.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3155) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 638, 1126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2914) [M::mem_pestat] mean and std.dev: (683.76, 517.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3808) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1227.693 CPU sec, 73.312 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (136, 938193, 72, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 114, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (119.89, 66.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 61, 1217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3581) [M::mem_pestat] mean and std.dev: (396.91, 793.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4763) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 269, 896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2276) [M::mem_pestat] mean and std.dev: (510.25, 443.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2966) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1219.252 CPU sec, 53.636 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (142, 938769, 92, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 128, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (122.89, 72.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 62, 1350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3980) [M::mem_pestat] mean and std.dev: (550.85, 1026.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5295) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 683, 1110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2874) [M::mem_pestat] mean and std.dev: (778.04, 652.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3756) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1211.686 CPU sec, 53.396 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (113, 938226, 77, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 101, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (116.03, 73.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 56, 1029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3023) [M::mem_pestat] mean and std.dev: (320.98, 715.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4020) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 244, 790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2008) [M::mem_pestat] mean and std.dev: (403.71, 425.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2617) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1241.273 CPU sec, 76.758 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (119, 939304, 77, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 133, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 586) [M::mem_pestat] mean and std.dev: (132.27, 97.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 50, 350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 988) [M::mem_pestat] mean and std.dev: (107.68, 187.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1307) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 240, 587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1415) [M::mem_pestat] mean and std.dev: (363.76, 350.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1829) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1218.476 CPU sec, 53.942 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (121, 938201, 85, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 119, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (119.99, 66.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.93) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 66, 1606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4734) [M::mem_pestat] mean and std.dev: (743.56, 1276.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6298) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 271, 832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2122) [M::mem_pestat] mean and std.dev: (476.31, 502.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2767) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1249.175 CPU sec, 75.313 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (154, 938626, 85, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 108, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (118.32, 72.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 58, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1376) [M::mem_pestat] mean and std.dev: (91.58, 180.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1824) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 308, 1101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2891) [M::mem_pestat] mean and std.dev: (560.57, 527.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3786) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1199.793 CPU sec, 52.778 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (130, 938331, 84, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 131, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (121.10, 74.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.16, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 61, 1574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4646) [M::mem_pestat] mean and std.dev: (683.71, 1277.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6182) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 274, 856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2282) [M::mem_pestat] mean and std.dev: (440.92, 444.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2995) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1259.870 CPU sec, 75.773 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (142, 938745, 69, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 122, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (123.11, 72.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 47, 89) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 215) [M::mem_pestat] mean and std.dev: (39.41, 24.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 278) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 519, 1014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2732) [M::mem_pestat] mean and std.dev: (650.03, 604.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3591) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1206.976 CPU sec, 53.169 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (125, 938193, 91, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 104, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (110.48, 72.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 57, 1774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5270) [M::mem_pestat] mean and std.dev: (820.99, 1474.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7018) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 282, 1057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2815) [M::mem_pestat] mean and std.dev: (588.06, 577.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3694) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1264.749 CPU sec, 74.740 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (122, 938652, 80, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 134, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (131.00, 75.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.12, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 51, 78) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 162) [M::mem_pestat] mean and std.dev: (46.62, 20.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 204) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 371, 1537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4319) [M::mem_pestat] mean and std.dev: (657.10, 707.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5710) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1223.998 CPU sec, 55.825 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (122, 938473, 92, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (85, 130, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (132.37, 73.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 57, 871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2555) [M::mem_pestat] mean and std.dev: (226.92, 509.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3397) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 279, 1343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3635) [M::mem_pestat] mean and std.dev: (857.27, 1008.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4892) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1235.297 CPU sec, 73.064 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 938762, 62, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 110, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (113.36, 67.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 56, 79) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 175) [M::mem_pestat] mean and std.dev: (49.27, 29.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 223) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 422, 976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2498) [M::mem_pestat] mean and std.dev: (624.31, 605.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3259) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1207.556 CPU sec, 53.420 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (130, 938867, 70, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 139, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (127.64, 76.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 54, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1775) [M::mem_pestat] mean and std.dev: (198.43, 445.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2356) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 334, 1269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3471) [M::mem_pestat] mean and std.dev: (738.28, 844.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4572) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1238.076 CPU sec, 74.246 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 939099, 85, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (80, 146, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (138.38, 76.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 62, 536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1548) [M::mem_pestat] mean and std.dev: (149.19, 319.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2054) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 237, 1075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2847) [M::mem_pestat] mean and std.dev: (624.19, 689.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3733) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1214.314 CPU sec, 53.498 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (138, 938502, 84, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 126, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (114.78, 67.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.82) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 56, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (56.22, 48.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 297, 876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2276) [M::mem_pestat] mean and std.dev: (496.49, 408.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2976) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1208.617 CPU sec, 54.645 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (123, 938043, 95, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 122, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (117.04, 69.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 52, 109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 257) [M::mem_pestat] mean and std.dev: (52.92, 42.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 331) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 1065, 1495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4057) [M::mem_pestat] mean and std.dev: (942.92, 733.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5338) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1256.230 CPU sec, 76.488 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (115, 938236, 67, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 112, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (115.92, 63.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 57, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 654) [M::mem_pestat] mean and std.dev: (68.30, 73.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 271, 915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2337) [M::mem_pestat] mean and std.dev: (434.17, 464.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3048) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1216.747 CPU sec, 54.097 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (122, 938862, 60, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 131, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 510) [M::mem_pestat] mean and std.dev: (131.28, 84.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 656) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 61, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 697) [M::mem_pestat] mean and std.dev: (76.29, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 294, 1001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2661) [M::mem_pestat] mean and std.dev: (546.88, 525.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3491) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1231.902 CPU sec, 74.172 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (130, 938292, 79, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 128, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (125.29, 66.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 51, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 634) [M::mem_pestat] mean and std.dev: (67.52, 109.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (120, 193, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1335) [M::mem_pestat] mean and std.dev: (322.65, 332.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1740) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1217.337 CPU sec, 53.641 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (132, 938887, 70, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 130, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (126.17, 76.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 63, 1350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3970) [M::mem_pestat] mean and std.dev: (422.93, 838.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5280) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 855, 1423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3867) [M::mem_pestat] mean and std.dev: (853.88, 700.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5089) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1246.593 CPU sec, 75.212 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (131, 938196, 93, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 117, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (118.08, 70.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 47, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (98.08, 183.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1260) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 246, 948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2520) [M::mem_pestat] mean and std.dev: (558.18, 569.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3306) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1232.209 CPU sec, 54.380 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (122, 939214, 63, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 127, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (123.77, 64.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 61, 742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2162) [M::mem_pestat] mean and std.dev: (162.14, 251.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2872) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 660, 1242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3346) [M::mem_pestat] mean and std.dev: (844.05, 888.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4399) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1256.460 CPU sec, 74.740 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (122, 938907, 72, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 113, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (116.22, 71.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 57, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1243) [M::mem_pestat] mean and std.dev: (128.08, 240.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1648) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 434, 1695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4693) [M::mem_pestat] mean and std.dev: (809.47, 881.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6192) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1211.417 CPU sec, 55.239 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (130, 938619, 67, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 122, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (122.77, 67.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 51, 1944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5760) [M::mem_pestat] mean and std.dev: (940.06, 1559.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7668) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 565, 1721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4837) [M::mem_pestat] mean and std.dev: (927.74, 1089.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6395) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1231.230 CPU sec, 74.489 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (131, 938661, 78, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 128, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (123.12, 72.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.11, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 47, 82) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 196) [M::mem_pestat] mean and std.dev: (43.13, 28.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 253) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 681, 1437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3903) [M::mem_pestat] mean and std.dev: (863.48, 921.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5136) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1202.398 CPU sec, 53.161 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (161, 938591, 81, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 137, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (137.29, 92.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 49, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 260) [M::mem_pestat] mean and std.dev: (51.22, 43.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 242, 1089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2967) [M::mem_pestat] mean and std.dev: (528.55, 576.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3906) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1231.570 CPU sec, 73.898 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (116, 937679, 73, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (88, 141, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (128.33, 63.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.11, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 68, 632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1822) [M::mem_pestat] mean and std.dev: (204.30, 383.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2417) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (273, 838, 2117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5805) [M::mem_pestat] mean and std.dev: (1266.73, 1224.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7649) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1209.293 CPU sec, 53.283 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (132, 938736, 79, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 119, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (129.52, 84.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.01, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 56, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1517) [M::mem_pestat] mean and std.dev: (69.72, 114.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2011) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (119, 268, 1343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3791) [M::mem_pestat] mean and std.dev: (636.31, 704.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5015) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1215.301 CPU sec, 56.850 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (121, 938768, 81, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 121, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (123.01, 72.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.13, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 50, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 188) [M::mem_pestat] mean and std.dev: (46.77, 27.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 240) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (247, 544, 1521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4069) [M::mem_pestat] mean and std.dev: (874.83, 834.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5343) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1246.641 CPU sec, 76.519 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (155, 938484, 84, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 124, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (128.01, 84.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.10, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 45, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 255) [M::mem_pestat] mean and std.dev: (46.66, 40.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 332) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (197, 368, 1255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3371) [M::mem_pestat] mean and std.dev: (734.00, 747.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4429) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1214.002 CPU sec, 53.778 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (130, 939608, 87, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 109, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (113.72, 66.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 50, 79) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 173) [M::mem_pestat] mean and std.dev: (42.13, 24.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 220) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 236, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (229.00, 152.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 989) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1221.515 CPU sec, 71.063 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (142, 938001, 66, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 116, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (126.58, 76.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 57, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (51.88, 46.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 260, 925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2345) [M::mem_pestat] mean and std.dev: (544.97, 541.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3055) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1201.399 CPU sec, 52.881 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (147, 938562, 87, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 121, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 447) [M::mem_pestat] mean and std.dev: (119.85, 77.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.11, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 67, 1962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5814) [M::mem_pestat] mean and std.dev: (939.61, 1491.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7740) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 305, 1127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2959) [M::mem_pestat] mean and std.dev: (695.66, 738.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3875) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1224.580 CPU sec, 67.644 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 939902, 85, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 110, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (120.46, 79.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 51, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 558) [M::mem_pestat] mean and std.dev: (65.06, 88.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 235, 931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2431) [M::mem_pestat] mean and std.dev: (481.44, 389.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3181) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1204.312 CPU sec, 52.993 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (123, 939996, 73, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 130, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (117.01, 64.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 53, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 197) [M::mem_pestat] mean and std.dev: (45.45, 28.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 254) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 313, 1029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2719) [M::mem_pestat] mean and std.dev: (552.70, 542.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3564) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1268.992 CPU sec, 74.606 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (138, 938686, 74, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 132, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (130.06, 76.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 62, 1957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5795) [M::mem_pestat] mean and std.dev: (900.21, 1564.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7714) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 261, 884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2266) [M::mem_pestat] mean and std.dev: (470.60, 410.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2957) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1222.359 CPU sec, 56.111 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 938536, 81, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 131, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (126.98, 78.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.10, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 48, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 345) [M::mem_pestat] mean and std.dev: (62.55, 58.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 252, 810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2146) [M::mem_pestat] mean and std.dev: (395.77, 371.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2814) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1234.329 CPU sec, 73.158 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (127, 939474, 83, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 135, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (126.65, 71.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 55, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (77.36, 112.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 813) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 237, 869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2341) [M::mem_pestat] mean and std.dev: (518.60, 538.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3077) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1211.284 CPU sec, 53.707 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (134, 938376, 85, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 129, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (126.44, 68.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (347.98, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 54, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (65.46, 66.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 282, 1011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2715) [M::mem_pestat] mean and std.dev: (618.80, 612.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3567) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1228.051 CPU sec, 71.614 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (144, 938248, 73, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 138, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (134.94, 77.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 57, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1426) [M::mem_pestat] mean and std.dev: (131.52, 247.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1891) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 254, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1966) [M::mem_pestat] mean and std.dev: (424.13, 354.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2559) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1212.448 CPU sec, 53.576 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (127, 939129, 77, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 121, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (114.38, 63.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 64, 1093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3217) [M::mem_pestat] mean and std.dev: (356.72, 633.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4279) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 234, 746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1900) [M::mem_pestat] mean and std.dev: (437.22, 435.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2477) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1226.330 CPU sec, 59.311 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (131, 938284, 82, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 105, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (104.48, 64.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 55, 85) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 203) [M::mem_pestat] mean and std.dev: (44.51, 27.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 262) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 281, 971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2549) [M::mem_pestat] mean and std.dev: (594.00, 654.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3338) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1265.608 CPU sec, 74.412 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (117, 938616, 67, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 120, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 375) [M::mem_pestat] mean and std.dev: (119.04, 66.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 76, 2077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6147) [M::mem_pestat] mean and std.dev: (975.30, 1510.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8182) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 305, 1032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2800) [M::mem_pestat] mean and std.dev: (561.82, 486.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3684) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1220.992 CPU sec, 55.813 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (106, 938170, 73, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 126, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (119.24, 69.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 62, 2544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7566) [M::mem_pestat] mean and std.dev: (1164.34, 1841.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10077) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 296, 1031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2667) [M::mem_pestat] mean and std.dev: (614.12, 657.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3485) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1226.646 CPU sec, 73.335 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (143, 938547, 68, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 115, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (121.69, 70.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 67, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1143) [M::mem_pestat] mean and std.dev: (81.60, 98.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1510) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 214, 1217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3343) [M::mem_pestat] mean and std.dev: (561.08, 603.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4406) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1222.982 CPU sec, 54.075 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (124, 938206, 85, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 111, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (107.32, 64.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 55, 216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (61.62, 59.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 332, 1073) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2917) [M::mem_pestat] mean and std.dev: (625.00, 574.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3839) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1231.709 CPU sec, 74.041 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (143, 938208, 75, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 133, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (130.29, 75.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 583) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 57, 78) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 176) [M::mem_pestat] mean and std.dev: (45.18, 25.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 225) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 322, 1269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3419) [M::mem_pestat] mean and std.dev: (599.30, 642.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4494) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1219.811 CPU sec, 53.888 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (134, 938629, 87, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 135, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (126.19, 66.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 63, 936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2738) [M::mem_pestat] mean and std.dev: (279.64, 550.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3639) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 227, 1030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2824) [M::mem_pestat] mean and std.dev: (520.32, 493.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3721) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1259.400 CPU sec, 74.282 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (132, 939145, 71, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 121, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 460) [M::mem_pestat] mean and std.dev: (121.55, 75.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.11, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 53, 383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1079) [M::mem_pestat] mean and std.dev: (79.02, 120.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1427) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 224, 925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2489) [M::mem_pestat] mean and std.dev: (515.03, 508.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3271) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1221.925 CPU sec, 55.748 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (127, 938947, 79, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 105, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (112.39, 72.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 59, 1304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3828) [M::mem_pestat] mean and std.dev: (546.96, 1043.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5090) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 593, 1171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3117) [M::mem_pestat] mean and std.dev: (634.65, 513.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4090) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1220.153 CPU sec, 73.073 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (135, 938842, 71, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 134, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 505) [M::mem_pestat] mean and std.dev: (129.31, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 58, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 622) [M::mem_pestat] mean and std.dev: (70.47, 91.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 821) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 245, 865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2175) [M::mem_pestat] mean and std.dev: (428.97, 400.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2830) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1214.316 CPU sec, 53.555 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (134, 939640, 55, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 101, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (103.33, 57.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.83) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 64, 2692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7996) [M::mem_pestat] mean and std.dev: (999.06, 1614.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10648) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (250, 807, 1781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4843) [M::mem_pestat] mean and std.dev: (862.11, 725.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6374) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1243.676 CPU sec, 74.717 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (127, 938212, 64, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 117, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (117.03, 69.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.01, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 58, 1126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3314) [M::mem_pestat] mean and std.dev: (375.23, 808.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4408) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 255, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1488) [M::mem_pestat] mean and std.dev: (338.32, 347.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1923) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1221.205 CPU sec, 53.925 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (136, 939297, 67, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 117, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (118.86, 71.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 51, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (51.72, 33.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 280, 1139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3109) [M::mem_pestat] mean and std.dev: (663.61, 761.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4094) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1224.074 CPU sec, 61.123 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (125, 938885, 84, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 104, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (118.34, 77.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.83) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 56, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 176) [M::mem_pestat] mean and std.dev: (44.79, 25.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 224) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 255, 1410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3904) [M::mem_pestat] mean and std.dev: (796.82, 939.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5151) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1272.354 CPU sec, 77.264 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 938570, 75, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 120, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (123.81, 70.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 58, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1551) [M::mem_pestat] mean and std.dev: (166.02, 303.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2055) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 583, 996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2614) [M::mem_pestat] mean and std.dev: (610.04, 546.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3423) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1209.415 CPU sec, 53.436 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (126, 939131, 86, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 112, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (114.59, 69.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.11, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 63, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2148) [M::mem_pestat] mean and std.dev: (179.86, 417.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2854) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 316, 1384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3734) [M::mem_pestat] mean and std.dev: (882.31, 954.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4909) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1239.319 CPU sec, 74.356 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (132, 938732, 76, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 111, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (123.17, 80.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 44, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 507) [M::mem_pestat] mean and std.dev: (57.89, 73.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 257, 1282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3460) [M::mem_pestat] mean and std.dev: (615.97, 693.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4549) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1210.640 CPU sec, 53.294 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (141, 938364, 76, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 103, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (113.89, 71.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 49, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 217) [M::mem_pestat] mean and std.dev: (42.29, 24.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 280) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 338, 1422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3844) [M::mem_pestat] mean and std.dev: (576.92, 683.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5055) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1233.839 CPU sec, 73.967 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (129, 938508, 77, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 131, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (133.63, 81.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 63, 1046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3064) [M::mem_pestat] mean and std.dev: (380.00, 769.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4073) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 264, 1252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3414) [M::mem_pestat] mean and std.dev: (529.64, 555.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4495) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1223.769 CPU sec, 53.889 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (147, 937927, 80, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 115, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (116.04, 70.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 65, 743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2159) [M::mem_pestat] mean and std.dev: (250.31, 500.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2867) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 214, 691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1781) [M::mem_pestat] mean and std.dev: (403.42, 427.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2326) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1251.942 CPU sec, 74.366 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (127, 938932, 69, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 106, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (123.86, 88.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 53, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (56.87, 51.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (132, 224, 785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2091) [M::mem_pestat] mean and std.dev: (405.25, 388.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2744) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1212.038 CPU sec, 55.129 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (155, 938878, 68, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 139, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (134.47, 71.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.01, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 63, 1625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4811) [M::mem_pestat] mean and std.dev: (540.38, 1248.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6404) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 490, 1192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3244) [M::mem_pestat] mean and std.dev: (705.76, 736.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4270) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1239.982 CPU sec, 72.847 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (116, 937888, 77, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 136, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (131.83, 76.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 50, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 223) [M::mem_pestat] mean and std.dev: (52.21, 39.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 288) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 226, 825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2177) [M::mem_pestat] mean and std.dev: (463.48, 534.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2853) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1221.013 CPU sec, 54.147 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (136, 938244, 65, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 116, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (119.07, 78.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.96) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 65, 2010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5964) [M::mem_pestat] mean and std.dev: (810.66, 1350.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7941) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 248, 698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1746) [M::mem_pestat] mean and std.dev: (427.59, 379.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2270) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1251.949 CPU sec, 75.210 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (143, 938931, 99, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 124, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 442) [M::mem_pestat] mean and std.dev: (121.92, 70.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 50, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (60.60, 64.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 220, 922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2440) [M::mem_pestat] mean and std.dev: (472.19, 549.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3199) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1220.196 CPU sec, 53.817 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (153, 938425, 64, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 114, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (114.46, 68.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 43, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 472) [M::mem_pestat] mean and std.dev: (53.14, 48.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 259, 775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1909) [M::mem_pestat] mean and std.dev: (450.38, 402.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2476) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1217.601 CPU sec, 62.437 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (127, 938727, 73, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 115, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (114.15, 73.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 66, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (87.02, 87.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 993) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 296, 1101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3037) [M::mem_pestat] mean and std.dev: (580.72, 507.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4005) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1235.443 CPU sec, 76.707 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 939366, 70, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 127, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 483) [M::mem_pestat] mean and std.dev: (128.58, 75.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.11, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 55, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 192) [M::mem_pestat] mean and std.dev: (53.81, 32.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 243) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 231, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1328) [M::mem_pestat] mean and std.dev: (329.61, 269.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1714) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1207.015 CPU sec, 53.308 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (124, 938607, 82, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 119, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (115.76, 67.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 67, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1388) [M::mem_pestat] mean and std.dev: (110.38, 177.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1838) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 239, 674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1682) [M::mem_pestat] mean and std.dev: (389.50, 381.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2186) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1235.121 CPU sec, 74.121 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (139, 939027, 71, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 119, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (118.89, 65.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 47, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 236) [M::mem_pestat] mean and std.dev: (43.82, 35.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 305) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (127, 200, 1089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3013) [M::mem_pestat] mean and std.dev: (577.00, 701.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3975) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1221.814 CPU sec, 53.816 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 938458, 82, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 110, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (116.27, 78.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 62, 1856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5490) [M::mem_pestat] mean and std.dev: (811.20, 1410.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7307) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 211, 1619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4535) [M::mem_pestat] mean and std.dev: (698.28, 891.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5993) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1237.450 CPU sec, 72.906 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (120, 938407, 79, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 97, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (104.50, 62.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 69, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 605) [M::mem_pestat] mean and std.dev: (96.08, 113.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 338, 1028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2638) [M::mem_pestat] mean and std.dev: (546.59, 520.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3443) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1215.708 CPU sec, 53.546 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (139, 938358, 74, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 127, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (121.58, 69.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 66, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1631) [M::mem_pestat] mean and std.dev: (130.95, 268.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2164) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 279, 1510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4192) [M::mem_pestat] mean and std.dev: (805.77, 902.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5533) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1257.511 CPU sec, 74.138 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (156, 937587, 71, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 125, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (125.01, 75.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 56, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 606) [M::mem_pestat] mean and std.dev: (73.72, 103.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (176, 294, 841) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2171) [M::mem_pestat] mean and std.dev: (497.60, 513.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2836) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.327 CPU sec, 56.268 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (153, 938303, 70, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 142, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (139.89, 78.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 57, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 964) [M::mem_pestat] mean and std.dev: (82.75, 140.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1276) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 270, 1143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3069) [M::mem_pestat] mean and std.dev: (695.88, 718.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4032) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1222.748 CPU sec, 61.151 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (122, 939210, 79, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 122, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (125.02, 72.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.81) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 53, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (68.45, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (120, 195, 985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2715) [M::mem_pestat] mean and std.dev: (512.96, 649.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3580) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1208.592 CPU sec, 53.145 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (141, 938687, 68, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 127, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (131.26, 74.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.83) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 58, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 691) [M::mem_pestat] mean and std.dev: (80.64, 109.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (165, 210, 894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2352) [M::mem_pestat] mean and std.dev: (383.73, 353.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3081) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1255.327 CPU sec, 74.465 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (137, 939303, 73, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (52, 111, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (113.97, 74.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.13, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 51, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (65.38, 78.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 423, 1102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2914) [M::mem_pestat] mean and std.dev: (613.00, 650.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3820) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1215.824 CPU sec, 53.755 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (139, 937930, 71, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 131, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (127.61, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 580) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 68, 1763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5203) [M::mem_pestat] mean and std.dev: (863.69, 1510.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6923) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 200, 603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1525) [M::mem_pestat] mean and std.dev: (253.59, 210.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1986) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1232.270 CPU sec, 63.515 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (139, 938490, 93, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 129, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (122.29, 67.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 57, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (85.30, 116.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 317, 1040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2774) [M::mem_pestat] mean and std.dev: (697.97, 730.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3641) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1257.747 CPU sec, 74.862 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (145, 939497, 65, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 121, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (120.57, 75.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (347.97, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 53, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 348) [M::mem_pestat] mean and std.dev: (56.21, 49.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 294, 1357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3685) [M::mem_pestat] mean and std.dev: (786.82, 781.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4849) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1227.256 CPU sec, 55.981 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (128, 937695, 89, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 131, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (124.66, 74.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 46, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 255) [M::mem_pestat] mean and std.dev: (47.46, 37.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 331) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 836, 1207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3193) [M::mem_pestat] mean and std.dev: (805.69, 617.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4186) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1239.508 CPU sec, 72.379 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (129, 938609, 82, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 122, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (125.91, 84.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.01, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 44, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154) [M::mem_pestat] mean and std.dev: (40.94, 24.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 197) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 337, 805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2083) [M::mem_pestat] mean and std.dev: (442.50, 373.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2722) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1214.763 CPU sec, 53.631 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (137, 938919, 92, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 120, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (123.96, 77.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 71, 976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2866) [M::mem_pestat] mean and std.dev: (309.97, 619.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3811) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 235, 789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2023) [M::mem_pestat] mean and std.dev: (455.47, 458.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2640) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1239.322 CPU sec, 70.096 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (123, 939582, 53, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 124, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (123.22, 64.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.94) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 59, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 645) [M::mem_pestat] mean and std.dev: (61.05, 56.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 225, 1019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2717) [M::mem_pestat] mean and std.dev: (550.67, 589.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3566) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1224.535 CPU sec, 54.077 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (150, 939443, 62, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 118, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (119.38, 73.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 61, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1316) [M::mem_pestat] mean and std.dev: (83.53, 145.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1746) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 230, 645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1543) [M::mem_pestat] mean and std.dev: (432.52, 363.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1992) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1255.929 CPU sec, 74.805 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (124, 939243, 78, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 107, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (114.06, 67.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (347.99, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 69, 1402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4140) [M::mem_pestat] mean and std.dev: (442.12, 855.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5509) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 200, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (165.76, 57.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1229.803 CPU sec, 55.958 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (146, 938643, 71, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 127, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (127.49, 69.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.83) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 56, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (85.93, 131.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1134) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 262, 917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2413) [M::mem_pestat] mean and std.dev: (404.86, 374.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3161) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1237.505 CPU sec, 74.378 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (147, 938325, 59, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 124, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (120.68, 75.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.93) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 57, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 827) [M::mem_pestat] mean and std.dev: (104.19, 166.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (187, 238, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1834) [M::mem_pestat] mean and std.dev: (370.32, 360.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2383) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1209.696 CPU sec, 53.512 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (141, 938717, 59, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 128, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (131.74, 86.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.96) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 57, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (50.91, 29.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (114, 176, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1350) [M::mem_pestat] mean and std.dev: (237.44, 230.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1762) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1244.904 CPU sec, 73.609 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (167, 938521, 67, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 121, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (130.11, 76.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.93) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 55, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (58.82, 53.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1146) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 305, 1250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3340) [M::mem_pestat] mean and std.dev: (558.84, 604.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4385) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1216.176 CPU sec, 53.688 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 939560, 78, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 115, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (118.97, 69.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 49, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (56.29, 44.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 934, 1909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5239) [M::mem_pestat] mean and std.dev: (1234.37, 1148.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6904) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1224.257 CPU sec, 66.281 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (137, 938388, 72, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 119, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (122.85, 74.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 65, 647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1859) [M::mem_pestat] mean and std.dev: (155.83, 295.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2465) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (201, 328, 1351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3651) [M::mem_pestat] mean and std.dev: (749.93, 797.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4801) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1239.654 CPU sec, 76.623 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 939230, 63, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 131, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (122.64, 71.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 61, 884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2568) [M::mem_pestat] mean and std.dev: (236.30, 466.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3410) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 251, 845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2207) [M::mem_pestat] mean and std.dev: (535.24, 543.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2888) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1208.095 CPU sec, 53.127 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (139, 938693, 77, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 130, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (121.70, 78.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 55, 347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 979) [M::mem_pestat] mean and std.dev: (82.74, 128.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1295) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 281, 981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2609) [M::mem_pestat] mean and std.dev: (688.32, 683.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3423) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1260.717 CPU sec, 74.275 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (119, 938558, 62, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 127, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (122.66, 67.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.14, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 60, 2241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6655) [M::mem_pestat] mean and std.dev: (948.28, 1626.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (185, 540, 833) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2129) [M::mem_pestat] mean and std.dev: (526.40, 430.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2777) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1207.201 CPU sec, 53.091 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (132, 937929, 86, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 126, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (119.75, 74.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 58, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 733) [M::mem_pestat] mean and std.dev: (82.01, 85.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 964) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 819, 1967) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5449) [M::mem_pestat] mean and std.dev: (965.62, 941.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7190) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1254.441 CPU sec, 75.996 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (146, 939162, 55, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 130, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (121.27, 71.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 60, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 257) [M::mem_pestat] mean and std.dev: (51.18, 34.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 212, 1039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2845) [M::mem_pestat] mean and std.dev: (335.28, 355.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3748) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1223.820 CPU sec, 53.961 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (127, 939733, 68, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 119, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (114.31, 61.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 55, 1991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5905) [M::mem_pestat] mean and std.dev: (823.78, 1417.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 294, 1818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5012) [M::mem_pestat] mean and std.dev: (861.22, 1043.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6609) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1201.384 CPU sec, 52.832 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (143, 938669, 74, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 114, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (112.54, 59.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 45, 92) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 210) [M::mem_pestat] mean and std.dev: (49.41, 38.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 269) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 639, 1398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3750) [M::mem_pestat] mean and std.dev: (758.90, 753.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4926) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1251.375 CPU sec, 76.676 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (117, 938732, 78, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 109, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (110.69, 67.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 63, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (61.73, 46.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 326, 776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2016) [M::mem_pestat] mean and std.dev: (431.97, 384.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2636) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1223.093 CPU sec, 54.707 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (118, 939070, 82, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 120, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (119.95, 68.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 55, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (66.72, 66.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 214, 1177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3229) [M::mem_pestat] mean and std.dev: (607.80, 682.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4255) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1230.071 CPU sec, 72.124 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (132, 938201, 74, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 126, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (137.16, 92.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.93) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 57, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (64.00, 54.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 256, 1077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2805) [M::mem_pestat] mean and std.dev: (555.33, 504.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3669) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1220.216 CPU sec, 53.824 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (120, 938246, 64, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 132, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 512) [M::mem_pestat] mean and std.dev: (131.17, 81.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 57, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (75.92, 90.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 246, 905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2401) [M::mem_pestat] mean and std.dev: (505.50, 546.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3149) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1226.271 CPU sec, 72.556 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (129, 938467, 55, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 109, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (115.69, 71.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 50, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2140) [M::mem_pestat] mean and std.dev: (133.40, 314.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2842) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 232, 689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1729) [M::mem_pestat] mean and std.dev: (395.25, 363.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2249) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1216.299 CPU sec, 53.539 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (130, 938877, 74, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 137, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (129.42, 71.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 66, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 244) [M::mem_pestat] mean and std.dev: (57.05, 44.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 314) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 244, 1080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2878) [M::mem_pestat] mean and std.dev: (418.44, 415.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3777) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1277.052 CPU sec, 74.716 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 939314, 74, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 127, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (120.31, 69.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 60, 109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 263) [M::mem_pestat] mean and std.dev: (51.82, 31.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 233, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 938) [M::mem_pestat] mean and std.dev: (244.96, 169.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1193) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1227.860 CPU sec, 55.755 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (145, 938545, 60, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 120, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (117.52, 68.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.12, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 57, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 659) [M::mem_pestat] mean and std.dev: (66.40, 87.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 417, 927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2391) [M::mem_pestat] mean and std.dev: (586.47, 518.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3123) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1227.851 CPU sec, 72.926 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (119, 937959, 70, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 125, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (123.23, 67.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.82) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 55, 658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1914) [M::mem_pestat] mean and std.dev: (148.44, 336.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2542) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 266, 867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2213) [M::mem_pestat] mean and std.dev: (570.96, 529.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2886) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1216.057 CPU sec, 53.961 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (145, 938402, 62, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 137, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 516) [M::mem_pestat] mean and std.dev: (125.56, 80.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 662) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 57, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (79.94, 107.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 296, 1174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3102) [M::mem_pestat] mean and std.dev: (612.16, 520.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4066) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1236.416 CPU sec, 73.747 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (134, 938617, 70, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 124, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (123.44, 71.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.81) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 65, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1263) [M::mem_pestat] mean and std.dev: (107.30, 199.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1676) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 240, 911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2387) [M::mem_pestat] mean and std.dev: (455.40, 471.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3125) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1219.784 CPU sec, 53.706 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (110, 938326, 88, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 114, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (117.84, 72.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.15, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 57, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 701) [M::mem_pestat] mean and std.dev: (62.90, 88.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 923) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 297, 1013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2579) [M::mem_pestat] mean and std.dev: (553.86, 481.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3362) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1204.944 CPU sec, 53.454 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (156, 938343, 79, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 117, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (115.16, 69.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.11, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 65, 1079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3169) [M::mem_pestat] mean and std.dev: (334.30, 751.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4214) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 250, 799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2075) [M::mem_pestat] mean and std.dev: (450.75, 430.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2713) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1250.686 CPU sec, 74.761 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 938566, 59, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 101, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (108.23, 68.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (347.99, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 58, 1218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3590) [M::mem_pestat] mean and std.dev: (405.38, 837.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4776) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 252, 1021) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2745) [M::mem_pestat] mean and std.dev: (615.38, 699.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3607) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1231.423 CPU sec, 56.005 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (124, 938997, 67, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 136, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (135.96, 88.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 72, 1861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5493) [M::mem_pestat] mean and std.dev: (821.90, 1434.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7309) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 494, 1083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2757) [M::mem_pestat] mean and std.dev: (536.88, 381.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3594) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1229.246 CPU sec, 73.140 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (138, 938458, 83, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 142, 219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (132.20, 77.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.11, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 52, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (56.47, 58.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 269, 1150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3124) [M::mem_pestat] mean and std.dev: (588.23, 646.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4111) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1208.352 CPU sec, 53.562 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (135, 938966, 74, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 123, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (124.02, 68.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 54, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 247) [M::mem_pestat] mean and std.dev: (54.82, 39.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 319) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 226, 867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2277) [M::mem_pestat] mean and std.dev: (439.83, 418.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2982) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1231.119 CPU sec, 68.586 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (127, 938310, 81, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 138, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (128.75, 73.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 54, 685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1997) [M::mem_pestat] mean and std.dev: (184.30, 398.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2653) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 230, 1220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3272) [M::mem_pestat] mean and std.dev: (620.86, 747.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4298) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1207.286 CPU sec, 53.091 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 939451, 70, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 121, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (122.51, 78.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 54, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (47.70, 36.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 391, 1450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3930) [M::mem_pestat] mean and std.dev: (765.70, 808.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5170) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1236.638 CPU sec, 73.227 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (150, 938118, 65, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 123, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 446) [M::mem_pestat] mean and std.dev: (118.02, 65.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 57, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (49.56, 25.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 394, 823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2041) [M::mem_pestat] mean and std.dev: (516.00, 367.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2650) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1204.483 CPU sec, 54.873 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (138, 939321, 84, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 121, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (114.00, 58.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.01, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 63, 112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (62.42, 50.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 355) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 546, 1253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3501) [M::mem_pestat] mean and std.dev: (708.71, 733.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4625) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1226.913 CPU sec, 71.245 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (124, 938805, 59, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 127, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (131.47, 86.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.01, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 61, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (67.49, 59.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 256, 660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1592) [M::mem_pestat] mean and std.dev: (396.62, 318.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2058) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1203.597 CPU sec, 53.388 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (123, 938663, 65, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (87, 143, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (131.93, 78.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 60, 2656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7920) [M::mem_pestat] mean and std.dev: (1131.95, 1975.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10552) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 251, 1201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3225) [M::mem_pestat] mean and std.dev: (620.21, 698.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4237) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1230.834 CPU sec, 71.144 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (146, 938821, 79, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 121, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (126.89, 81.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (347.98, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 72, 1165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3409) [M::mem_pestat] mean and std.dev: (402.84, 701.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4531) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 293, 854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2208) [M::mem_pestat] mean and std.dev: (546.39, 483.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2885) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1204.333 CPU sec, 53.073 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (124, 938312, 74, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 105, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (109.30, 65.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 50, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1342) [M::mem_pestat] mean and std.dev: (101.08, 184.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1779) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 715, 1010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2612) [M::mem_pestat] mean and std.dev: (629.11, 467.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3413) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1201.054 CPU sec, 55.554 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (115, 939630, 82, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 104, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (115.92, 69.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 64, 693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2013) [M::mem_pestat] mean and std.dev: (130.73, 287.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2673) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 273, 1273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3431) [M::mem_pestat] mean and std.dev: (755.19, 949.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4553) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1261.100 CPU sec, 74.461 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (115, 938554, 74, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 121, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (121.22, 72.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 73, 2015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5961) [M::mem_pestat] mean and std.dev: (882.75, 1348.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7934) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 326, 956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2422) [M::mem_pestat] mean and std.dev: (598.61, 554.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3155) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1204.412 CPU sec, 55.128 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (122, 938971, 78, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 129, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 455) [M::mem_pestat] mean and std.dev: (133.10, 74.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 51, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 215) [M::mem_pestat] mean and std.dev: (46.45, 30.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 276) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 251, 812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2094) [M::mem_pestat] mean and std.dev: (468.86, 429.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2735) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1223.930 CPU sec, 71.865 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (128, 939335, 62, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 91, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (108.70, 66.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 478) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.10, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 59, 856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2500) [M::mem_pestat] mean and std.dev: (190.48, 427.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3322) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 291, 1026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2766) [M::mem_pestat] mean and std.dev: (542.30, 533.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3636) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1195.572 CPU sec, 52.871 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (127, 937885, 70, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 100, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (110.88, 76.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 69, 1546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4568) [M::mem_pestat] mean and std.dev: (769.67, 1323.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6079) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 343, 1145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3017) [M::mem_pestat] mean and std.dev: (630.94, 637.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3953) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1226.218 CPU sec, 71.709 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 938858, 69, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 126, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (129.87, 75.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 61, 688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1998) [M::mem_pestat] mean and std.dev: (100.82, 205.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2653) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 275, 924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2394) [M::mem_pestat] mean and std.dev: (548.00, 559.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3129) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1196.274 CPU sec, 52.814 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (127, 939238, 59, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 135, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 497) [M::mem_pestat] mean and std.dev: (138.51, 87.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 58, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (58.34, 50.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (182, 272, 760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1916) [M::mem_pestat] mean and std.dev: (451.42, 456.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2494) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1236.830 CPU sec, 73.278 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (125, 938445, 99, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 129, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 449) [M::mem_pestat] mean and std.dev: (130.24, 79.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.93) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 56, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 515) [M::mem_pestat] mean and std.dev: (61.54, 63.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 298, 1210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3308) [M::mem_pestat] mean and std.dev: (644.81, 617.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4357) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1199.786 CPU sec, 54.779 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (130, 938630, 86, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 127, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (119.52, 68.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 62, 754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2200) [M::mem_pestat] mean and std.dev: (133.04, 330.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2923) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 224, 625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1533) [M::mem_pestat] mean and std.dev: (359.43, 365.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1987) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1223.389 CPU sec, 72.346 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 938896, 85, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 134, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (122.98, 66.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.84) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 59, 624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1808) [M::mem_pestat] mean and std.dev: (151.87, 330.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2400) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 542, 897) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2269) [M::mem_pestat] mean and std.dev: (527.68, 447.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2955) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1196.217 CPU sec, 52.978 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (142, 938897, 73, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (75, 128, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (123.46, 69.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 54, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (57.75, 63.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1462) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 356, 1179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3201) [M::mem_pestat] mean and std.dev: (690.97, 707.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4212) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1218.247 CPU sec, 69.362 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (138, 938287, 64, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 127, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (121.74, 67.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 60, 1128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3324) [M::mem_pestat] mean and std.dev: (413.37, 803.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4422) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 291, 786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2062) [M::mem_pestat] mean and std.dev: (424.43, 357.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2700) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1188.262 CPU sec, 52.471 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (128, 939126, 77, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 95, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (101.93, 57.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.93) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 46, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 263) [M::mem_pestat] mean and std.dev: (50.44, 40.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (245, 578, 1788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4874) [M::mem_pestat] mean and std.dev: (889.52, 882.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6417) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1188.848 CPU sec, 56.310 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (126, 939342, 68, 28) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 107, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (122.08, 80.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (347.97, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 53, 781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2279) [M::mem_pestat] mean and std.dev: (161.75, 434.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3028) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (199, 310, 1122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2968) [M::mem_pestat] mean and std.dev: (620.27, 632.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3891) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.404 CPU sec, 73.376 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (141, 939090, 69, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 133, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (133.42, 79.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 62, 1919) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5693) [M::mem_pestat] mean and std.dev: (803.00, 1431.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7580) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (195, 272, 837) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2121) [M::mem_pestat] mean and std.dev: (544.73, 534.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2763) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1193.063 CPU sec, 54.663 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (134, 938199, 67, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 138, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (130.02, 73.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.01, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 50, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 222) [M::mem_pestat] mean and std.dev: (42.58, 22.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 285) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (291, 677, 1203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3027) [M::mem_pestat] mean and std.dev: (784.68, 587.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3939) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1215.754 CPU sec, 71.052 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (122, 939309, 80, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 123, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (121.49, 70.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 53, 2037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6057) [M::mem_pestat] mean and std.dev: (857.39, 1637.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8067) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 273, 1040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2760) [M::mem_pestat] mean and std.dev: (776.73, 791.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3942) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1181.208 CPU sec, 52.155 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (156, 939150, 73, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 125, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (118.58, 74.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.10, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 62, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (75.61, 90.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 250, 1088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2918) [M::mem_pestat] mean and std.dev: (628.66, 729.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3833) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1206.268 CPU sec, 70.817 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (110, 938526, 90, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 100, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (103.33, 65.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 55, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (53.64, 48.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (155, 240, 926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2468) [M::mem_pestat] mean and std.dev: (377.18, 400.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3239) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1184.156 CPU sec, 52.230 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (123, 938942, 74, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 117, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (118.23, 72.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.10, 34.83) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 50, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (45.21, 24.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 265, 1131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3107) [M::mem_pestat] mean and std.dev: (646.00, 732.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1225.105 CPU sec, 73.232 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (120, 939086, 71, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (78, 135, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (130.84, 67.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 55, 92) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 200) [M::mem_pestat] mean and std.dev: (46.15, 23.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 254) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 247, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1562) [M::mem_pestat] mean and std.dev: (334.53, 256.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2019) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1185.753 CPU sec, 54.485 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (131, 939210, 77, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 124, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (122.80, 72.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 56, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (53.77, 38.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 431, 1611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4419) [M::mem_pestat] mean and std.dev: (877.96, 964.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5823) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1204.776 CPU sec, 69.077 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (130, 939510, 72, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 129, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (128.69, 69.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 54, 608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1754) [M::mem_pestat] mean and std.dev: (68.45, 97.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2327) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 255, 1307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3511) [M::mem_pestat] mean and std.dev: (639.55, 670.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4613) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1181.385 CPU sec, 52.350 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 938229, 68, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 120, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (124.21, 74.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 68, 865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2531) [M::mem_pestat] mean and std.dev: (188.85, 418.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3364) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 243, 1538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4258) [M::mem_pestat] mean and std.dev: (818.68, 926.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5618) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1207.764 CPU sec, 69.369 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (124, 938736, 69, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 133, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 457) [M::mem_pestat] mean and std.dev: (123.26, 74.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 54, 1116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3282) [M::mem_pestat] mean and std.dev: (272.07, 659.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4365) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 287, 870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2272) [M::mem_pestat] mean and std.dev: (425.15, 360.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2973) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1185.587 CPU sec, 52.190 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (116, 938259, 69, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 123, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (120.16, 67.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 68, 1317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3883) [M::mem_pestat] mean and std.dev: (420.49, 821.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5166) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (268, 859, 1590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4234) [M::mem_pestat] mean and std.dev: (882.00, 745.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5556) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1190.272 CPU sec, 58.150 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (130, 939123, 65, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 123, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (123.52, 72.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 616) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 56, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 352) [M::mem_pestat] mean and std.dev: (54.08, 45.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 264, 943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2461) [M::mem_pestat] mean and std.dev: (531.31, 529.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3220) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1208.486 CPU sec, 73.469 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (137, 938417, 68, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 128, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (122.93, 65.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (347.97, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 57, 669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1947) [M::mem_pestat] mean and std.dev: (189.33, 381.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2586) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 343, 1011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2625) [M::mem_pestat] mean and std.dev: (675.10, 616.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3432) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1188.719 CPU sec, 52.788 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (146, 938932, 72, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 128, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (121.36, 68.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 58, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (62.61, 63.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 647, 1195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3243) [M::mem_pestat] mean and std.dev: (736.03, 725.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4267) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1211.425 CPU sec, 69.845 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (139, 939307, 86, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 112, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (122.91, 64.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 60, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1539) [M::mem_pestat] mean and std.dev: (195.24, 379.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2041) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 249, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (244.12, 114.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1186.996 CPU sec, 52.221 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (132, 938807, 85, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 124, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (123.72, 74.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.81) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 55, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (60.15, 51.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 463) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 244, 1082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2942) [M::mem_pestat] mean and std.dev: (643.57, 698.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3872) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1200.094 CPU sec, 69.544 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (134, 938763, 62, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 108, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (112.76, 68.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.13, 34.83) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 74, 1071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3141) [M::mem_pestat] mean and std.dev: (309.58, 656.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4176) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 282, 918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2438) [M::mem_pestat] mean and std.dev: (472.70, 463.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3198) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1185.143 CPU sec, 52.275 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (132, 939626, 86, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 115, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (113.78, 71.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 50, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 214) [M::mem_pestat] mean and std.dev: (46.91, 24.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 274) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 469, 901) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2319) [M::mem_pestat] mean and std.dev: (618.33, 587.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3028) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1224.731 CPU sec, 72.509 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (141, 937935, 74, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (53, 113, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (117.44, 69.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 57, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (60.68, 61.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 876) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 250, 1124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3012) [M::mem_pestat] mean and std.dev: (697.84, 803.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3956) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1187.993 CPU sec, 54.395 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (149, 939082, 75, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 105, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 487) [M::mem_pestat] mean and std.dev: (115.64, 77.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 43, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 610) [M::mem_pestat] mean and std.dev: (63.85, 85.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 804) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 370, 1319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3579) [M::mem_pestat] mean and std.dev: (785.76, 802.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4709) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1199.011 CPU sec, 68.983 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (153, 939122, 79, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 105, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (111.03, 67.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 55, 233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (64.05, 83.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 325, 1424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3840) [M::mem_pestat] mean and std.dev: (909.44, 980.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5048) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1181.102 CPU sec, 52.163 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (126, 938926, 60, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 115, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (115.33, 65.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 66, 1257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3703) [M::mem_pestat] mean and std.dev: (530.98, 955.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4926) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 214, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 532) [M::mem_pestat] mean and std.dev: (176.68, 72.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1194.379 CPU sec, 65.740 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (110, 939449, 80, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 114, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (118.50, 66.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 55, 912) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2676) [M::mem_pestat] mean and std.dev: (191.37, 488.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3558) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 293, 1438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3902) [M::mem_pestat] mean and std.dev: (760.84, 809.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5134) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1182.248 CPU sec, 52.065 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (136, 938317, 84, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 135, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (133.62, 73.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.02, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 56, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (65.72, 68.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 226, 910) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2368) [M::mem_pestat] mean and std.dev: (430.68, 530.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3097) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1196.122 CPU sec, 59.950 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (144, 938694, 79, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 122, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (122.86, 68.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.08, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 62, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1574) [M::mem_pestat] mean and std.dev: (156.60, 306.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2086) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 249, 798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2114) [M::mem_pestat] mean and std.dev: (419.74, 448.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2772) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1214.298 CPU sec, 74.100 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (150, 938441, 76, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (86, 137, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (135.82, 83.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.15, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 62, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1705) [M::mem_pestat] mean and std.dev: (157.65, 283.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2261) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (128, 291, 1244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3476) [M::mem_pestat] mean and std.dev: (734.83, 791.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4592) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1183.225 CPU sec, 52.215 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (141, 939002, 78, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 97, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 459) [M::mem_pestat] mean and std.dev: (116.07, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 62, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (74.34, 76.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (160, 231, 867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2281) [M::mem_pestat] mean and std.dev: (418.26, 406.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2988) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1204.734 CPU sec, 70.423 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (131, 939196, 81, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 115, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (113.78, 69.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 67, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1553) [M::mem_pestat] mean and std.dev: (142.79, 204.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2060) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 683, 1357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3689) [M::mem_pestat] mean and std.dev: (816.50, 884.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4855) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1179.446 CPU sec, 51.917 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (127, 938280, 86, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (72, 118, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (124.25, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.06, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 60, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (59.55, 61.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 917) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (118, 232, 910) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2494) [M::mem_pestat] mean and std.dev: (514.22, 654.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3286) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1206.461 CPU sec, 70.873 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 938332, 80, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 112, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (120.87, 78.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 577) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (347.98, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 46, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (49.08, 54.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 650, 1221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3237) [M::mem_pestat] mean and std.dev: (676.79, 602.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4245) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1182.846 CPU sec, 52.042 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (140, 938612, 69, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 129, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 493) [M::mem_pestat] mean and std.dev: (127.90, 71.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 50, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 621) [M::mem_pestat] mean and std.dev: (68.96, 85.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 353, 974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2582) [M::mem_pestat] mean and std.dev: (513.93, 501.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3386) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1234.106 CPU sec, 73.065 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (143, 938277, 59, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 125, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (124.99, 70.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 48, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (55.24, 45.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (179, 243, 785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1997) [M::mem_pestat] mean and std.dev: (437.25, 407.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2603) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1185.106 CPU sec, 54.154 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (138, 938780, 71, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 131, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (126.25, 71.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.01, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 52, 82) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 170) [M::mem_pestat] mean and std.dev: (47.04, 22.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 214) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 219, 1198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3328) [M::mem_pestat] mean and std.dev: (576.29, 710.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4393) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1212.991 CPU sec, 72.321 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (131, 938988, 75, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 124, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (121.30, 65.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 95, 2391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7097) [M::mem_pestat] mean and std.dev: (969.67, 1326.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9450) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 262, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2307) [M::mem_pestat] mean and std.dev: (429.60, 421.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3020) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1182.308 CPU sec, 52.519 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (118, 938488, 79, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 121, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (121.47, 66.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.01, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 50, 97) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 225) [M::mem_pestat] mean and std.dev: (45.00, 26.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 289) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 265, 2515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7259) [M::mem_pestat] mean and std.dev: (883.46, 1243.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9631) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1209.064 CPU sec, 69.664 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (120, 938219, 77, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 101, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (106.22, 63.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.10, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 50, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (60.87, 94.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 910) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 229, 1563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4393) [M::mem_pestat] mean and std.dev: (726.28, 1093.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5808) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1183.272 CPU sec, 52.458 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (139, 938701, 84, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (74, 130, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (128.96, 75.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.87) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 51, 1956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5810) [M::mem_pestat] mean and std.dev: (859.84, 1507.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7737) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 266, 703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1801) [M::mem_pestat] mean and std.dev: (417.13, 413.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2350) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1195.936 CPU sec, 62.049 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (133, 938486, 83, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 127, 200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (123.89, 69.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 57, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1091) [M::mem_pestat] mean and std.dev: (73.89, 127.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1442) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 241, 1540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4280) [M::mem_pestat] mean and std.dev: (642.82, 779.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5650) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1214.205 CPU sec, 72.930 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (120, 939641, 69, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (76, 130, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (122.01, 65.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.90) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 51, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (69.30, 103.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1250) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 271, 1107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2939) [M::mem_pestat] mean and std.dev: (561.75, 594.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3855) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1180.652 CPU sec, 52.123 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (114, 938549, 69, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 113, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 367) [M::mem_pestat] mean and std.dev: (120.07, 67.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.79) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 67, 1652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4874) [M::mem_pestat] mean and std.dev: (766.98, 1292.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6485) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 246, 1093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2941) [M::mem_pestat] mean and std.dev: (557.76, 508.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3865) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1207.046 CPU sec, 70.268 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (130, 938145, 75, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 130, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 486) [M::mem_pestat] mean and std.dev: (122.84, 71.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 63, 1314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3868) [M::mem_pestat] mean and std.dev: (553.07, 1045.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5145) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 225, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (217.67, 122.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1181.008 CPU sec, 52.038 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (121, 937576, 62, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 122, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (118.40, 63.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.00, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 44, 112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (45.71, 42.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 373) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 268, 1156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3082) [M::mem_pestat] mean and std.dev: (653.00, 736.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4045) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1207.327 CPU sec, 70.853 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (142, 938406, 64, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (84, 145, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 522) [M::mem_pestat] mean and std.dev: (143.12, 81.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.95) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 51, 82) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 196) [M::mem_pestat] mean and std.dev: (42.96, 27.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 253) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 246, 810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2034) [M::mem_pestat] mean and std.dev: (474.88, 498.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2646) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1186.149 CPU sec, 52.367 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (122, 938811, 76, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 123, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (123.38, 78.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.05, 34.88) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 55, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (56.81, 59.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (171, 429, 1480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4098) [M::mem_pestat] mean and std.dev: (914.18, 1070.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5407) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1232.120 CPU sec, 72.815 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (148, 938683, 65, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 124, 194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (128.85, 73.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.04, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 69, 2336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6914) [M::mem_pestat] mean and std.dev: (809.78, 1649.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9203) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 566, 1432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3936) [M::mem_pestat] mean and std.dev: (841.10, 898.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5188) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1189.994 CPU sec, 54.178 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (129, 939003, 79, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 115, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (118.83, 70.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.94) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 59, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (61.74, 56.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 272, 1161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3101) [M::mem_pestat] mean and std.dev: (686.98, 741.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4071) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1200.497 CPU sec, 68.561 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (125, 939137, 74, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 136, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (129.69, 74.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.92) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 59, 1978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5856) [M::mem_pestat] mean and std.dev: (929.24, 1675.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7795) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 259, 939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2397) [M::mem_pestat] mean and std.dev: (529.82, 576.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3126) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1179.006 CPU sec, 52.234 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (139, 938712, 58, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (54, 117, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (110.98, 73.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.07, 34.89) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 61, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 252) [M::mem_pestat] mean and std.dev: (52.11, 38.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 326) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 461, 1111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2905) [M::mem_pestat] mean and std.dev: (654.25, 613.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3802) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1200.362 CPU sec, 68.620 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (122, 938888, 78, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (56, 129, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (116.45, 73.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.09, 34.91) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 48, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (57.21, 55.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 265, 870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2278) [M::mem_pestat] mean and std.dev: (515.09, 474.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2982) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1183.636 CPU sec, 52.246 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 1179280 sequences (117928000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (119, 939676, 92, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (59, 120, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 473) [M::mem_pestat] mean and std.dev: (122.17, 76.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (348.03, 34.85) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 59, 1358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4010) [M::mem_pestat] mean and std.dev: (309.58, 704.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5336) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 251, 821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2101) [M::mem_pestat] mean and std.dev: (460.57, 510.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2741) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1197.768 CPU sec, 62.546 real sec [M::process] 0 single-end sequences; 1179280 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (60, 461026, 37, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (57, 129, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (117.87, 67.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 347, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (237, 457) [M::mem_pestat] mean and std.dev: (347.91, 34.86) [M::mem_pestat] low and high boundaries for proper pairs: (193, 501) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 57, 586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1668) [M::mem_pestat] mean and std.dev: (121.43, 295.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2209) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 227, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1496) [M::mem_pestat] mean and std.dev: (319.58, 268.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1939) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1179280 reads in 588.019 CPU sec, 25.869 real sec [main] Version: 0.7.17-r1188 [main] CMD: /home/csyk2proj/tools/bwa.kit/bwa mem -p -t24 -R@RG\tID:KPGP-00254\tPL:ILLUMINA\tSM:KPGP-00254 -C /home/csyk2proj/data/bwa.kit/hs38DH.fa - [main] Real time: 55792.667 sec; CPU: 625874.166 sec