[M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938764, 30, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (297, 364, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 864) [M::mem_pestat] mean and std.dev: (328.41, 127.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.32, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (151, 1689, 4420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12958) [M::mem_pestat] mean and std.dev: (2414.60, 2682.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17227) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 371, 630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1358) [M::mem_pestat] mean and std.dev: (331.50, 131.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1722) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1289.664 CPU sec, 75.528 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 939410, 28, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 367, 883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2137) [M::mem_pestat] mean and std.dev: (525.86, 391.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.33, 84.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (99, 276, 2068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6006) [M::mem_pestat] mean and std.dev: (1019.00, 1397.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7975) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (274, 723, 3097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8743) [M::mem_pestat] mean and std.dev: (2021.86, 2718.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12897) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1240.494 CPU sec, 56.119 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938406, 40, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 376, 1148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2940) [M::mem_pestat] mean and std.dev: (589.65, 639.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3836) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.49, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 1193, 2727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8069) [M::mem_pestat] mean and std.dev: (1275.06, 1667.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10740) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (291, 735, 1664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4410) [M::mem_pestat] mean and std.dev: (624.94, 460.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5783) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1263.206 CPU sec, 71.450 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 938153, 41, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 311, 1067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2727) [M::mem_pestat] mean and std.dev: (380.43, 266.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.60, 84.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 369, 2715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8049) [M::mem_pestat] mean and std.dev: (1268.85, 1389.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10716) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (437, 992, 1668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4130) [M::mem_pestat] mean and std.dev: (1262.94, 1039.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5423) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1237.307 CPU sec, 54.730 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 938051, 39, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (306, 545, 1174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2910) [M::mem_pestat] mean and std.dev: (785.53, 641.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.46, 84.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 133, 1748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5140) [M::mem_pestat] mean and std.dev: (617.44, 1084.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6836) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 363, 1898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5344) [M::mem_pestat] mean and std.dev: (855.56, 1119.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7067) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1269.984 CPU sec, 72.942 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938736, 41, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (341, 499, 826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1796) [M::mem_pestat] mean and std.dev: (513.59, 341.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2281) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.53, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 190, 2711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8033) [M::mem_pestat] mean and std.dev: (1259.37, 1638.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10694) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2400000 reads in 1240.418 CPU sec, 54.567 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 937815, 35, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (306, 491, 1034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2490) [M::mem_pestat] mean and std.dev: (601.00, 507.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3218) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.32, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (84, 514, 3251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9585) [M::mem_pestat] mean and std.dev: (1708.94, 1964.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12752) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 1446, 2724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7786) [M::mem_pestat] mean and std.dev: (1616.80, 1732.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1272.352 CPU sec, 58.410 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 938261, 34, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 335, 1163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3007) [M::mem_pestat] mean and std.dev: (688.81, 601.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3929) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.28, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (67, 2043, 3768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11170) [M::mem_pestat] mean and std.dev: (2215.71, 2226.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14871) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1282.004 CPU sec, 77.760 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 938918, 33, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (347, 426, 1878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4940) [M::mem_pestat] mean and std.dev: (1079.67, 1242.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6471) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.35, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (58, 188, 2862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8470) [M::mem_pestat] mean and std.dev: (1266.58, 1536.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11274) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 322, 1126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2858) [M::mem_pestat] mean and std.dev: (540.95, 456.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3724) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1251.496 CPU sec, 55.312 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938482, 36, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 293, 768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1910) [M::mem_pestat] mean and std.dev: (420.75, 343.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.41, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (81, 754, 2714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7980) [M::mem_pestat] mean and std.dev: (1525.03, 1867.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10613) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 456, 1071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2803) [M::mem_pestat] mean and std.dev: (410.70, 295.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3669) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1281.082 CPU sec, 76.007 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 937519, 40, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (331, 432, 1063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2527) [M::mem_pestat] mean and std.dev: (468.82, 302.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3259) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.38, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (79, 1524, 3040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8962) [M::mem_pestat] mean and std.dev: (1909.00, 2122.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11923) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (158, 535, 1477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4115) [M::mem_pestat] mean and std.dev: (900.67, 860.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5434) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1263.725 CPU sec, 55.636 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 938364, 30, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 282, 1176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3026) [M::mem_pestat] mean and std.dev: (675.41, 669.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3951) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.47, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (86, 1953, 2675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7853) [M::mem_pestat] mean and std.dev: (1748.14, 1674.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10442) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 832, 1174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3048) [M::mem_pestat] mean and std.dev: (845.95, 813.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4098) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1276.100 CPU sec, 74.350 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 938434, 37, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 354, 723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1767) [M::mem_pestat] mean and std.dev: (399.42, 359.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2289) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (108, 1204, 4600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13584) [M::mem_pestat] mean and std.dev: (2858.30, 3265.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18076) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 911, 2289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6405) [M::mem_pestat] mean and std.dev: (1517.18, 1821.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8801) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1242.193 CPU sec, 54.683 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 937848, 35, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 346, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1784) [M::mem_pestat] mean and std.dev: (391.50, 243.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2308) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.45, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 291, 3023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8983) [M::mem_pestat] mean and std.dev: (1858.86, 2653.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12474) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 322, 706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1626) [M::mem_pestat] mean and std.dev: (521.00, 442.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2289) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1269.055 CPU sec, 62.640 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938483, 40, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (372, 449, 969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2163) [M::mem_pestat] mean and std.dev: (616.72, 448.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.30, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 927, 3070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9136) [M::mem_pestat] mean and std.dev: (1997.35, 2607.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12426) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (291, 398, 844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1950) [M::mem_pestat] mean and std.dev: (553.69, 377.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2503) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1300.636 CPU sec, 77.925 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938675, 33, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (279, 418, 2465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6837) [M::mem_pestat] mean and std.dev: (1298.00, 1641.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9023) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.40, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (60, 1818, 4444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13212) [M::mem_pestat] mean and std.dev: (2215.52, 2429.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17596) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2400000 reads in 1262.796 CPU sec, 55.719 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 939060, 33, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 370, 1223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3221) [M::mem_pestat] mean and std.dev: (740.12, 777.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4220) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.49, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (73, 890, 3027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8935) [M::mem_pestat] mean and std.dev: (1981.18, 2314.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11889) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 297, 689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1611) [M::mem_pestat] mean and std.dev: (333.64, 285.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2072) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1297.087 CPU sec, 76.145 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 938085, 39, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 286, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 881) [M::mem_pestat] mean and std.dev: (282.83, 154.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 79, 868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2526) [M::mem_pestat] mean and std.dev: (278.58, 516.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3355) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (606, 894, 1287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2649) [M::mem_pestat] mean and std.dev: (838.00, 509.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3330) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1255.690 CPU sec, 55.230 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938316, 37, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 338, 1260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3284) [M::mem_pestat] mean and std.dev: (703.29, 713.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4296) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 196, 3440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10232) [M::mem_pestat] mean and std.dev: (1714.68, 2504.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13628) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 595, 2011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5513) [M::mem_pestat] mean and std.dev: (781.61, 691.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7264) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1280.222 CPU sec, 72.980 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 938847, 43, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 327, 749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1767) [M::mem_pestat] mean and std.dev: (498.64, 387.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2276) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.30, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (51, 1417, 3064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9090) [M::mem_pestat] mean and std.dev: (2108.43, 2543.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12282) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (265, 852, 2149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5917) [M::mem_pestat] mean and std.dev: (1064.83, 1024.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7801) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1262.761 CPU sec, 55.585 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938359, 45, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 485, 1234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3136) [M::mem_pestat] mean and std.dev: (648.05, 499.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4087) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.55, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (78, 194, 3068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9048) [M::mem_pestat] mean and std.dev: (1603.62, 2187.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12038) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (211, 744, 1269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3385) [M::mem_pestat] mean and std.dev: (714.27, 596.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4443) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1282.520 CPU sec, 67.232 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 937910, 51, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (242, 365, 979) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2453) [M::mem_pestat] mean and std.dev: (539.76, 451.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3190) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.32, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (71, 1472, 2875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8483) [M::mem_pestat] mean and std.dev: (1894.02, 2028.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11287) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 353, 955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2369) [M::mem_pestat] mean and std.dev: (412.21, 345.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3076) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1309.226 CPU sec, 78.521 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 939129, 40, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 325, 743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1781) [M::mem_pestat] mean and std.dev: (334.20, 229.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2300) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (76, 263, 4445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13183) [M::mem_pestat] mean and std.dev: (1995.62, 2628.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17552) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (273, 402, 1413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3693) [M::mem_pestat] mean and std.dev: (591.92, 583.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4833) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1261.380 CPU sec, 55.859 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 937791, 39, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 308, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1541) [M::mem_pestat] mean and std.dev: (324.26, 208.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1997) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.47, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (70, 767, 4087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12121) [M::mem_pestat] mean and std.dev: (2334.38, 2702.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16138) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (486, 767, 1850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4578) [M::mem_pestat] mean and std.dev: (781.50, 484.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5942) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1295.266 CPU sec, 74.937 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 939264, 34, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 412, 1228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3174) [M::mem_pestat] mean and std.dev: (683.00, 619.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4147) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (326, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (110, 650) [M::mem_pestat] mean and std.dev: (380.63, 84.06) [M::mem_pestat] low and high boundaries for proper pairs: (2, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (80, 1642, 3196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9428) [M::mem_pestat] mean and std.dev: (1846.32, 1938.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12544) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (482, 1220, 2393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6215) [M::mem_pestat] mean and std.dev: (1341.30, 1025.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8126) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1267.573 CPU sec, 55.757 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 938702, 37, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 323, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1736) [M::mem_pestat] mean and std.dev: (507.41, 428.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2221) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.48, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (61, 159, 2580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7618) [M::mem_pestat] mean and std.dev: (685.41, 1153.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10137) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (295, 678, 1120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2770) [M::mem_pestat] mean and std.dev: (636.76, 437.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3595) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1271.391 CPU sec, 74.129 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 937234, 36, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 362, 1047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2651) [M::mem_pestat] mean and std.dev: (635.22, 601.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (64, 223, 2985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8827) [M::mem_pestat] mean and std.dev: (1780.89, 2431.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11748) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (580, 702, 996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1828) [M::mem_pestat] mean and std.dev: (751.67, 348.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2244) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1277.731 CPU sec, 56.157 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938615, 41, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (308, 354, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1172) [M::mem_pestat] mean and std.dev: (404.72, 276.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.38, 84.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 61, 2754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8208) [M::mem_pestat] mean and std.dev: (1226.39, 1941.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10935) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 338, 999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2525) [M::mem_pestat] mean and std.dev: (589.53, 578.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3288) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1282.167 CPU sec, 70.620 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 938542, 35, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (278, 362, 909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2171) [M::mem_pestat] mean and std.dev: (485.14, 325.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.40, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (66, 1815, 3713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11007) [M::mem_pestat] mean and std.dev: (2034.29, 2319.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14654) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (975, 1559, 1890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3720) [M::mem_pestat] mean and std.dev: (1400.17, 706.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4635) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1273.534 CPU sec, 64.197 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938204, 39, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 377, 995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2489) [M::mem_pestat] mean and std.dev: (457.00, 412.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3236) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (65, 663, 3472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10286) [M::mem_pestat] mean and std.dev: (1833.59, 2456.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13693) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (281, 757, 1027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2519) [M::mem_pestat] mean and std.dev: (690.62, 512.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3265) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1265.244 CPU sec, 55.639 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938565, 29, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 312, 440) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 852) [M::mem_pestat] mean and std.dev: (332.35, 155.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.45, 84.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (62, 516, 2811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8309) [M::mem_pestat] mean and std.dev: (1353.39, 1484.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11058) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 279, 1068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2718) [M::mem_pestat] mean and std.dev: (464.07, 371.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3543) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1273.904 CPU sec, 74.544 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 939176, 37, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 382, 666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1488) [M::mem_pestat] mean and std.dev: (434.69, 277.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.28, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (94, 2016, 4479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13249) [M::mem_pestat] mean and std.dev: (2474.51, 2525.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17634) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (169, 999, 2861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8245) [M::mem_pestat] mean and std.dev: (1658.19, 1976.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10937) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1259.354 CPU sec, 55.376 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938526, 38, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 325, 642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1402) [M::mem_pestat] mean and std.dev: (411.30, 277.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1782) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (57, 228, 2800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8286) [M::mem_pestat] mean and std.dev: (1293.44, 1898.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11029) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (294, 929, 1753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4671) [M::mem_pestat] mean and std.dev: (909.69, 666.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6130) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1275.554 CPU sec, 75.173 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 938548, 37, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 371, 1632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4350) [M::mem_pestat] mean and std.dev: (697.00, 600.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (74, 496, 4457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13223) [M::mem_pestat] mean and std.dev: (2193.81, 2713.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17606) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 425, 1119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2921) [M::mem_pestat] mean and std.dev: (757.81, 699.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3822) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1258.049 CPU sec, 55.344 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 937664, 35, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 357, 557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1283) [M::mem_pestat] mean and std.dev: (329.89, 234.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1646) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (74, 1369, 2958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8726) [M::mem_pestat] mean and std.dev: (1947.29, 2140.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11610) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (284, 648, 1120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2792) [M::mem_pestat] mean and std.dev: (628.17, 487.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3628) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1289.173 CPU sec, 74.393 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938170, 38, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 389, 988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2380) [M::mem_pestat] mean and std.dev: (581.65, 400.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3076) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.47, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 437, 2851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8471) [M::mem_pestat] mean and std.dev: (1604.92, 2287.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11281) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (331, 602, 2016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5386) [M::mem_pestat] mean and std.dev: (884.82, 816.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7071) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1278.661 CPU sec, 71.301 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 938687, 39, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 317, 1221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3203) [M::mem_pestat] mean and std.dev: (613.67, 692.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4194) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.34, 84.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (86, 221, 2568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7532) [M::mem_pestat] mean and std.dev: (1062.28, 1505.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10014) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 499, 1201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3211) [M::mem_pestat] mean and std.dev: (607.21, 515.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4216) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1262.075 CPU sec, 55.450 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 938361, 50, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (268, 298, 785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1819) [M::mem_pestat] mean and std.dev: (430.00, 313.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.55, 84.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 1491, 2894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8586) [M::mem_pestat] mean and std.dev: (1748.86, 2031.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11432) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 1092, 1351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3693) [M::mem_pestat] mean and std.dev: (643.20, 516.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4864) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1293.489 CPU sec, 77.323 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 938026, 33, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 323, 1100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2776) [M::mem_pestat] mean and std.dev: (708.40, 732.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.34, 84.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 81, 3175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9451) [M::mem_pestat] mean and std.dev: (1681.12, 2550.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12589) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 307, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1188) [M::mem_pestat] mean and std.dev: (383.57, 290.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1546) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1268.031 CPU sec, 55.888 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938947, 26, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (342, 491, 1846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4854) [M::mem_pestat] mean and std.dev: (989.15, 994.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.57, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 73, 2573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7615) [M::mem_pestat] mean and std.dev: (1041.92, 1882.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10136) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (323, 1293, 2449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6701) [M::mem_pestat] mean and std.dev: (1407.43, 1286.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8827) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1302.740 CPU sec, 76.602 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 939453, 43, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 269, 564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1314) [M::mem_pestat] mean and std.dev: (299.53, 200.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.29, 84.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (62, 716, 3520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10436) [M::mem_pestat] mean and std.dev: (2051.58, 2538.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13894) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (261, 837, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2817) [M::mem_pestat] mean and std.dev: (787.58, 598.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3669) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1257.950 CPU sec, 55.370 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938115, 32, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 377, 1574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4240) [M::mem_pestat] mean and std.dev: (735.70, 923.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.35, 84.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 966, 3528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10484) [M::mem_pestat] mean and std.dev: (2085.50, 2537.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13962) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (303, 577, 1115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2739) [M::mem_pestat] mean and std.dev: (643.23, 508.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3551) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1292.211 CPU sec, 76.934 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938662, 35, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (276, 389, 1713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4587) [M::mem_pestat] mean and std.dev: (770.36, 738.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6024) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.32, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 169, 854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2480) [M::mem_pestat] mean and std.dev: (341.39, 555.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3293) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 378, 1465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3969) [M::mem_pestat] mean and std.dev: (625.69, 582.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5221) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1255.674 CPU sec, 64.202 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 939182, 41, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 343, 969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2509) [M::mem_pestat] mean and std.dev: (496.71, 379.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3279) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (200, 1218, 2155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6065) [M::mem_pestat] mean and std.dev: (1292.67, 1387.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8020) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (457, 1047, 1323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3055) [M::mem_pestat] mean and std.dev: (872.55, 470.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3921) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1249.922 CPU sec, 54.925 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938917, 37, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (135, 288, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (228.00, 107.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 963) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (192, 2550, 4458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12990) [M::mem_pestat] mean and std.dev: (2660.51, 2464.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17256) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 495, 1413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3729) [M::mem_pestat] mean and std.dev: (710.54, 664.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4887) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1286.729 CPU sec, 78.007 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 938952, 34, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 280, 822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2140) [M::mem_pestat] mean and std.dev: (415.07, 375.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.29, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 1774, 2920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8660) [M::mem_pestat] mean and std.dev: (1729.85, 1821.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11530) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 1209, 3082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8784) [M::mem_pestat] mean and std.dev: (1742.69, 1979.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11635) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1256.856 CPU sec, 55.280 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 938139, 43, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 298, 1281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3379) [M::mem_pestat] mean and std.dev: (714.12, 783.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (77, 173, 2974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8768) [M::mem_pestat] mean and std.dev: (1485.02, 2171.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11665) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 367, 725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1887) [M::mem_pestat] mean and std.dev: (418.57, 375.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2468) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1294.425 CPU sec, 76.163 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938724, 28, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 331, 1144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2994) [M::mem_pestat] mean and std.dev: (455.20, 416.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3919) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.53, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (49, 310, 4588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13666) [M::mem_pestat] mean and std.dev: (2613.39, 3325.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18205) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (238, 300, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1303) [M::mem_pestat] mean and std.dev: (439.62, 322.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1731) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1265.165 CPU sec, 55.649 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 938589, 37, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (266, 332, 1305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3383) [M::mem_pestat] mean and std.dev: (805.15, 845.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.38, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 184, 2832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8414) [M::mem_pestat] mean and std.dev: (1154.57, 1552.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11205) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (420, 1012, 2006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5178) [M::mem_pestat] mean and std.dev: (1318.06, 1124.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6764) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1304.208 CPU sec, 79.369 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 937941, 40, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 421, 1784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4840) [M::mem_pestat] mean and std.dev: (783.55, 820.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.41, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 1078, 4441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13231) [M::mem_pestat] mean and std.dev: (2223.78, 2507.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17626) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (352, 647, 989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2263) [M::mem_pestat] mean and std.dev: (769.91, 566.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3038) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1265.576 CPU sec, 55.602 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 938456, 30, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 323, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1350) [M::mem_pestat] mean and std.dev: (378.00, 283.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1730) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.30, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 158, 2645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7845) [M::mem_pestat] mean and std.dev: (1029.63, 1540.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10445) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (167, 280, 867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2267) [M::mem_pestat] mean and std.dev: (494.45, 426.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2967) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1301.122 CPU sec, 76.114 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938446, 28, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 324, 359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (285.31, 86.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 743) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.41, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (74, 1535, 4441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13175) [M::mem_pestat] mean and std.dev: (2293.64, 2550.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17542) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (960, 1343, 3121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7443) [M::mem_pestat] mean and std.dev: (1549.15, 1123.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9604) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1271.075 CPU sec, 57.935 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 939028, 31, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 414, 858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2048) [M::mem_pestat] mean and std.dev: (554.38, 469.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (81, 670, 3227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9519) [M::mem_pestat] mean and std.dev: (1848.52, 2238.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12665) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (259, 443, 1297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3373) [M::mem_pestat] mean and std.dev: (758.11, 599.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4411) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1271.299 CPU sec, 75.022 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938829, 40, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 538, 1213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3135) [M::mem_pestat] mean and std.dev: (629.33, 648.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4096) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.32, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 179, 2459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7319) [M::mem_pestat] mean and std.dev: (1039.39, 1611.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9749) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 498, 895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2153) [M::mem_pestat] mean and std.dev: (561.73, 361.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2782) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1267.791 CPU sec, 56.010 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 938380, 32, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 452, 1108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2908) [M::mem_pestat] mean and std.dev: (608.47, 578.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (57, 661, 3184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9438) [M::mem_pestat] mean and std.dev: (1915.00, 2627.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12565) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 270, 1017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2733) [M::mem_pestat] mean and std.dev: (443.85, 399.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3591) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1284.290 CPU sec, 76.362 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938238, 32, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 283, 643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1489) [M::mem_pestat] mean and std.dev: (251.40, 152.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1912) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.35, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 383, 2629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7779) [M::mem_pestat] mean and std.dev: (1293.84, 1800.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10354) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (305, 527, 1281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3233) [M::mem_pestat] mean and std.dev: (696.00, 494.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4209) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1278.742 CPU sec, 56.322 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938360, 35, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 379, 1172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2990) [M::mem_pestat] mean and std.dev: (644.20, 504.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.31, 84.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (74, 596, 1537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4463) [M::mem_pestat] mean and std.dev: (650.23, 833.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5926) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 674, 1569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4221) [M::mem_pestat] mean and std.dev: (889.44, 761.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5547) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1259.285 CPU sec, 59.997 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938204, 36, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 349, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1147) [M::mem_pestat] mean and std.dev: (359.53, 251.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1460) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.26, 84.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (66, 1268, 3532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10464) [M::mem_pestat] mean and std.dev: (2206.08, 2665.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13930) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (278, 556, 1341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3467) [M::mem_pestat] mean and std.dev: (859.05, 889.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4530) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1312.689 CPU sec, 79.321 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 938298, 38, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (276, 390, 1150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2898) [M::mem_pestat] mean and std.dev: (412.45, 273.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.36, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (62, 243, 2797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8267) [M::mem_pestat] mean and std.dev: (1320.69, 1689.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11002) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 310, 1777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4865) [M::mem_pestat] mean and std.dev: (1149.29, 1305.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6409) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1260.016 CPU sec, 55.605 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938792, 27, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (257, 339, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (9, 629) [M::mem_pestat] mean and std.dev: (295.57, 70.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.45, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 116, 3102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9232) [M::mem_pestat] mean and std.dev: (1620.23, 2526.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12297) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (499, 1138, 1238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2716) [M::mem_pestat] mean and std.dev: (876.50, 465.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3455) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1268.779 CPU sec, 75.176 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 938614, 48, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (288, 426, 575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1149) [M::mem_pestat] mean and std.dev: (368.33, 135.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.41, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (74, 1453, 4416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13100) [M::mem_pestat] mean and std.dev: (2098.92, 2229.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17442) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (292, 997, 1554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4078) [M::mem_pestat] mean and std.dev: (1090.50, 940.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5340) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1264.475 CPU sec, 55.614 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 939576, 43, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 312, 691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1757) [M::mem_pestat] mean and std.dev: (371.00, 324.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2290) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.46, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (57, 203, 4435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13191) [M::mem_pestat] mean and std.dev: (1953.47, 2565.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17569) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 786, 959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2431) [M::mem_pestat] mean and std.dev: (504.11, 348.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3167) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1301.072 CPU sec, 78.557 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938133, 30, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (294, 539, 1153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2871) [M::mem_pestat] mean and std.dev: (765.83, 777.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3877) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 681, 4453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13277) [M::mem_pestat] mean and std.dev: (2068.50, 2445.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17689) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (290, 783, 1505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3935) [M::mem_pestat] mean and std.dev: (880.50, 623.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5150) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1258.315 CPU sec, 55.345 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 938475, 39, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (280, 410, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1051) [M::mem_pestat] mean and std.dev: (343.23, 130.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1308) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.57, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 99, 1247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3677) [M::mem_pestat] mean and std.dev: (449.94, 760.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4892) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 483, 1031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2607) [M::mem_pestat] mean and std.dev: (515.31, 359.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3395) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1272.186 CPU sec, 64.989 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 939426, 31, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 384, 1299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3475) [M::mem_pestat] mean and std.dev: (637.45, 650.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.34, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (64, 675, 2992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8848) [M::mem_pestat] mean and std.dev: (1541.10, 1940.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11776) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (274, 405, 1124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2824) [M::mem_pestat] mean and std.dev: (549.29, 428.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3674) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1276.380 CPU sec, 71.251 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 938996, 47, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (249, 455, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2397) [M::mem_pestat] mean and std.dev: (676.79, 541.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3113) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.34, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (104, 1300, 3913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11531) [M::mem_pestat] mean and std.dev: (2123.21, 2431.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15340) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 510, 1107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3035) [M::mem_pestat] mean and std.dev: (773.38, 914.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4430) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1262.015 CPU sec, 55.539 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 938804, 26, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 324, 775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1779) [M::mem_pestat] mean and std.dev: (483.33, 387.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2281) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.35, 84.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 152, 1945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5769) [M::mem_pestat] mean and std.dev: (868.75, 1365.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7681) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (337, 772, 1067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2527) [M::mem_pestat] mean and std.dev: (750.24, 497.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3257) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1288.775 CPU sec, 77.715 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 938845, 33, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (290, 328, 855) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1985) [M::mem_pestat] mean and std.dev: (447.14, 331.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.49, 84.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 260, 2866) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8520) [M::mem_pestat] mean and std.dev: (1479.30, 1777.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11347) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (273, 705, 2394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6636) [M::mem_pestat] mean and std.dev: (1340.00, 1546.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8757) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1260.005 CPU sec, 55.460 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938308, 50, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (269, 439, 1806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4880) [M::mem_pestat] mean and std.dev: (834.80, 1047.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6417) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.50, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 272, 2991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8891) [M::mem_pestat] mean and std.dev: (1799.41, 2527.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11909) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (334, 462, 927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2113) [M::mem_pestat] mean and std.dev: (662.06, 522.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2752) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1291.465 CPU sec, 76.771 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 939022, 28, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 360, 940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2216) [M::mem_pestat] mean and std.dev: (461.00, 426.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.30, 84.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 89, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7716) [M::mem_pestat] mean and std.dev: (916.08, 1494.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10274) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 530, 1037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2669) [M::mem_pestat] mean and std.dev: (605.00, 426.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3485) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1258.586 CPU sec, 55.347 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 939344, 38, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 318, 1155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3017) [M::mem_pestat] mean and std.dev: (525.86, 547.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.49, 84.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (98, 1508, 3730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10994) [M::mem_pestat] mean and std.dev: (1890.58, 1780.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14626) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (510, 643, 2529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6567) [M::mem_pestat] mean and std.dev: (1747.14, 1847.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9139) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1292.548 CPU sec, 69.291 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938451, 36, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 324, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1852) [M::mem_pestat] mean and std.dev: (377.81, 236.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 513, 3584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10670) [M::mem_pestat] mean and std.dev: (1841.94, 2420.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14213) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (291, 1016, 1668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4422) [M::mem_pestat] mean and std.dev: (1063.76, 876.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5799) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1281.981 CPU sec, 76.516 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 938913, 48, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (272, 402, 1293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3335) [M::mem_pestat] mean and std.dev: (585.48, 491.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4356) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.26, 84.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 179, 1458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4284) [M::mem_pestat] mean and std.dev: (555.00, 894.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5697) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (347, 908, 1469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3713) [M::mem_pestat] mean and std.dev: (883.86, 753.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4835) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1263.470 CPU sec, 55.712 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 938365, 34, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 324, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 900) [M::mem_pestat] mean and std.dev: (263.46, 105.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1136) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.46, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (51, 954, 2673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7917) [M::mem_pestat] mean and std.dev: (1216.39, 1355.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10539) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (265, 784, 1707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4591) [M::mem_pestat] mean and std.dev: (636.07, 548.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6033) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1289.018 CPU sec, 76.470 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938480, 33, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 407, 1308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3466) [M::mem_pestat] mean and std.dev: (748.90, 779.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.36, 84.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 388, 3251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9691) [M::mem_pestat] mean and std.dev: (1873.52, 2486.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12911) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (363, 464, 1545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3909) [M::mem_pestat] mean and std.dev: (1018.45, 994.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5091) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1264.045 CPU sec, 55.586 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938169, 46, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 540, 1392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3522) [M::mem_pestat] mean and std.dev: (724.84, 565.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.30, 84.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (111, 1880, 4040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11898) [M::mem_pestat] mean and std.dev: (2200.07, 2419.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15827) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (355, 763, 1107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2611) [M::mem_pestat] mean and std.dev: (703.09, 549.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3363) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1274.411 CPU sec, 75.292 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938259, 33, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 331, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1335) [M::mem_pestat] mean and std.dev: (393.00, 272.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (51, 151, 1486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4356) [M::mem_pestat] mean and std.dev: (709.42, 1007.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5791) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (416, 1019, 1909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4895) [M::mem_pestat] mean and std.dev: (1047.70, 865.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6388) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1259.350 CPU sec, 55.394 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938124, 36, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 286, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (268.93, 76.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.30, 84.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (87, 792, 3556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10494) [M::mem_pestat] mean and std.dev: (1777.64, 2065.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13963) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 446, 852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2092) [M::mem_pestat] mean and std.dev: (477.79, 347.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2712) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1285.607 CPU sec, 73.205 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 937908, 33, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 314, 759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1923) [M::mem_pestat] mean and std.dev: (413.12, 415.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.59, 84.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 298, 2742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8148) [M::mem_pestat] mean and std.dev: (1662.70, 2245.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10851) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (340, 1142, 3375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9445) [M::mem_pestat] mean and std.dev: (1563.46, 1442.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12480) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1278.866 CPU sec, 69.254 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 938406, 32, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (272, 397, 895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2141) [M::mem_pestat] mean and std.dev: (529.38, 408.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.46, 84.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 235, 3577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10669) [M::mem_pestat] mean and std.dev: (1485.12, 2200.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14215) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (274, 610, 1757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4723) [M::mem_pestat] mean and std.dev: (1067.78, 1173.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6206) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1258.163 CPU sec, 55.319 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938655, 41, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 277, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (273.80, 122.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1074) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.50, 83.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 126, 2893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8609) [M::mem_pestat] mean and std.dev: (1409.08, 2034.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11467) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (387, 904, 1299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3123) [M::mem_pestat] mean and std.dev: (828.83, 509.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4035) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1296.880 CPU sec, 75.240 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 939176, 38, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 364, 957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2389) [M::mem_pestat] mean and std.dev: (498.64, 510.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3105) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.30, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 861, 1945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5741) [M::mem_pestat] mean and std.dev: (1009.03, 1182.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7639) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (415, 714, 1327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3151) [M::mem_pestat] mean and std.dev: (907.33, 665.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4063) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1273.165 CPU sec, 55.953 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 937929, 28, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (143, 823, 2305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6629) [M::mem_pestat] mean and std.dev: (1137.79, 1715.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.34, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (66, 1263, 2637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7779) [M::mem_pestat] mean and std.dev: (1332.73, 1470.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10350) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (529, 881, 1581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3685) [M::mem_pestat] mean and std.dev: (826.17, 444.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4737) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1270.755 CPU sec, 73.243 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 937529, 44, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 296, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1026) [M::mem_pestat] mean and std.dev: (279.95, 157.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.45, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (53, 153, 3449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10241) [M::mem_pestat] mean and std.dev: (1837.27, 2721.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13637) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (332, 737, 1501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3839) [M::mem_pestat] mean and std.dev: (775.50, 509.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5008) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1269.908 CPU sec, 55.809 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 938130, 29, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 313, 1001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2539) [M::mem_pestat] mean and std.dev: (483.09, 536.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3308) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (61, 432, 2536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7486) [M::mem_pestat] mean and std.dev: (1369.61, 2007.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9961) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2400000 reads in 1288.166 CPU sec, 75.935 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938955, 28, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (246, 317, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (298.95, 105.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (71, 1615, 4462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13244) [M::mem_pestat] mean and std.dev: (2603.68, 3174.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17635) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (355, 630, 1135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2695) [M::mem_pestat] mean and std.dev: (651.90, 422.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3475) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1272.579 CPU sec, 69.521 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 937897, 34, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (280, 372, 538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054) [M::mem_pestat] mean and std.dev: (328.32, 146.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1312) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (77, 1465, 3078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9080) [M::mem_pestat] mean and std.dev: (1802.44, 1766.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12081) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 308, 792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1922) [M::mem_pestat] mean and std.dev: (517.60, 442.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2487) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1270.615 CPU sec, 55.841 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 938058, 48, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 284, 608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1432) [M::mem_pestat] mean and std.dev: (368.62, 269.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.25, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 443, 4151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12387) [M::mem_pestat] mean and std.dev: (1935.69, 2449.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16505) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 533, 1310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3410) [M::mem_pestat] mean and std.dev: (691.07, 726.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4460) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1284.033 CPU sec, 75.985 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938179, 44, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (267, 313, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (285.29, 99.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 803) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.56, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (60, 397, 3642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10806) [M::mem_pestat] mean and std.dev: (1701.11, 2210.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14388) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (268, 675, 1421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3727) [M::mem_pestat] mean and std.dev: (835.00, 800.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4880) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1255.457 CPU sec, 55.192 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938405, 32, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (313, 454, 904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2086) [M::mem_pestat] mean and std.dev: (539.91, 399.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.29, 84.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 210, 2985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8859) [M::mem_pestat] mean and std.dev: (1559.72, 2197.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11796) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 319, 1030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2658) [M::mem_pestat] mean and std.dev: (555.58, 435.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3472) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1297.132 CPU sec, 78.264 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938846, 48, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 267, 642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1524) [M::mem_pestat] mean and std.dev: (305.81, 212.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.41, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 397, 2633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7837) [M::mem_pestat] mean and std.dev: (1466.15, 2104.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10439) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 432, 1476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3940) [M::mem_pestat] mean and std.dev: (510.27, 419.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5172) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1259.103 CPU sec, 55.391 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 937826, 44, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 459, 1217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3249) [M::mem_pestat] mean and std.dev: (626.24, 579.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4265) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.48, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 939, 3545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10531) [M::mem_pestat] mean and std.dev: (1884.07, 2234.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14024) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 421, 1020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2694) [M::mem_pestat] mean and std.dev: (626.14, 579.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3531) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1267.037 CPU sec, 75.121 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 938557, 38, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 296, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975) [M::mem_pestat] mean and std.dev: (374.00, 229.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1294) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.58, 84.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 143, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1150) [M::mem_pestat] mean and std.dev: (110.40, 114.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1521) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (213, 800, 1221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3237) [M::mem_pestat] mean and std.dev: (855.15, 775.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4245) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1293.349 CPU sec, 76.798 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938717, 33, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 520, 1299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3335) [M::mem_pestat] mean and std.dev: (794.77, 670.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.38, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 83, 1868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5536) [M::mem_pestat] mean and std.dev: (720.29, 1270.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7370) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (438, 1119, 1562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3810) [M::mem_pestat] mean and std.dev: (1156.36, 989.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5113) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1262.338 CPU sec, 55.594 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938889, 30, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 338, 628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1472) [M::mem_pestat] mean and std.dev: (400.05, 310.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1894) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (66, 2274, 4444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13200) [M::mem_pestat] mean and std.dev: (2368.37, 2686.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17578) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 416, 1485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4005) [M::mem_pestat] mean and std.dev: (733.87, 812.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5265) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1296.534 CPU sec, 76.593 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938760, 36, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 274, 903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2315) [M::mem_pestat] mean and std.dev: (360.94, 374.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3021) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.38, 84.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (67, 1969, 3639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10783) [M::mem_pestat] mean and std.dev: (2285.39, 2374.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14355) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (309, 1051, 1599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4179) [M::mem_pestat] mean and std.dev: (886.15, 623.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5469) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1263.849 CPU sec, 55.683 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 937884, 34, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 314, 1454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3808) [M::mem_pestat] mean and std.dev: (725.64, 767.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4985) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (66, 2408, 4439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13185) [M::mem_pestat] mean and std.dev: (2300.00, 2407.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17558) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (280, 461, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1954) [M::mem_pestat] mean and std.dev: (422.00, 241.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2512) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1303.792 CPU sec, 77.971 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 939192, 31, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (270, 391, 643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1389) [M::mem_pestat] mean and std.dev: (370.12, 220.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.38, 84.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (59, 370, 3562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10568) [M::mem_pestat] mean and std.dev: (2224.19, 2797.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14071) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (315, 1033, 1768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4674) [M::mem_pestat] mean and std.dev: (1032.53, 745.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6127) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1265.372 CPU sec, 55.748 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 938444, 43, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (323, 926, 1131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2747) [M::mem_pestat] mean and std.dev: (678.85, 404.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.50, 84.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (105, 1337, 4443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13119) [M::mem_pestat] mean and std.dev: (2267.44, 2481.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17457) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (282, 1058, 1740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4656) [M::mem_pestat] mean and std.dev: (896.79, 678.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6114) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1290.609 CPU sec, 74.894 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938885, 41, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 381, 979) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2339) [M::mem_pestat] mean and std.dev: (465.00, 355.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3019) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.34, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (70, 447, 3699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10957) [M::mem_pestat] mean and std.dev: (2010.22, 2496.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14586) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2400000 reads in 1268.147 CPU sec, 55.898 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 937958, 44, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 374, 971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2387) [M::mem_pestat] mean and std.dev: (452.00, 316.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3095) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.25, 84.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (49, 520, 2718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8056) [M::mem_pestat] mean and std.dev: (1349.19, 1581.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10725) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (320, 880, 1358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3434) [M::mem_pestat] mean and std.dev: (805.40, 545.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4472) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1292.624 CPU sec, 76.533 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938300, 24, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 323, 1423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3851) [M::mem_pestat] mean and std.dev: (744.41, 824.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5065) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.52, 84.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (55, 1721, 3643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10819) [M::mem_pestat] mean and std.dev: (2312.04, 2470.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14407) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 900, 2073) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5727) [M::mem_pestat] mean and std.dev: (1203.19, 1225.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7554) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1266.250 CPU sec, 57.308 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 938801, 23, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (282, 313, 888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2100) [M::mem_pestat] mean and std.dev: (417.83, 327.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.35, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 185, 2288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6784) [M::mem_pestat] mean and std.dev: (1020.48, 1502.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9032) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (358, 996, 1875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4909) [M::mem_pestat] mean and std.dev: (1188.50, 1110.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6426) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1273.251 CPU sec, 73.568 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 938742, 35, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 406, 1024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2576) [M::mem_pestat] mean and std.dev: (555.63, 488.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.47, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (63, 861, 4439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13191) [M::mem_pestat] mean and std.dev: (2118.83, 2627.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17567) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (299, 811, 1710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4532) [M::mem_pestat] mean and std.dev: (772.92, 692.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5943) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1266.094 CPU sec, 55.906 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 937876, 31, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 358, 1063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2809) [M::mem_pestat] mean and std.dev: (552.10, 574.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 240, 1848) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5458) [M::mem_pestat] mean and std.dev: (1001.13, 1371.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7263) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (726, 872, 1522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3114) [M::mem_pestat] mean and std.dev: (920.85, 611.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3910) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1305.423 CPU sec, 78.786 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 939223, 26, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 604, 1468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3878) [M::mem_pestat] mean and std.dev: (832.75, 742.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5083) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.24, 84.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (64, 313, 2252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6628) [M::mem_pestat] mean and std.dev: (1049.08, 1379.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8816) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1113, 1170, 1570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (199, 2484) [M::mem_pestat] mean and std.dev: (1293.22, 393.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2941) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1251.089 CPU sec, 55.037 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 938432, 41, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 247, 1265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3355) [M::mem_pestat] mean and std.dev: (624.53, 770.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4400) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.53, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 187, 2682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7988) [M::mem_pestat] mean and std.dev: (1207.10, 1689.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10641) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 446, 833) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2167) [M::mem_pestat] mean and std.dev: (541.14, 395.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2834) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1276.650 CPU sec, 62.738 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938317, 31, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 342, 1040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2654) [M::mem_pestat] mean and std.dev: (510.77, 394.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.36, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 205, 2587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7701) [M::mem_pestat] mean and std.dev: (1178.00, 1696.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10258) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (338, 923, 1383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3473) [M::mem_pestat] mean and std.dev: (834.07, 592.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4518) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1311.045 CPU sec, 76.755 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 938065, 44, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (269, 358, 756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1730) [M::mem_pestat] mean and std.dev: (435.93, 334.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2217) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.48, 84.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (188, 639, 2797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8015) [M::mem_pestat] mean and std.dev: (1418.91, 1726.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10624) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2400000 reads in 1265.490 CPU sec, 57.416 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 938263, 35, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 311, 897) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2329) [M::mem_pestat] mean and std.dev: (454.46, 400.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3045) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.34, 84.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (89, 1711, 4445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13157) [M::mem_pestat] mean and std.dev: (2355.17, 2467.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17513) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 632, 912) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2320) [M::mem_pestat] mean and std.dev: (647.71, 602.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3059) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1288.854 CPU sec, 76.918 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 938675, 30, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (266, 563, 1418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3722) [M::mem_pestat] mean and std.dev: (652.64, 611.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 570, 3194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9518) [M::mem_pestat] mean and std.dev: (2004.17, 2849.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13402) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 309, 1191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3177) [M::mem_pestat] mean and std.dev: (589.94, 583.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4170) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1267.171 CPU sec, 55.989 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938604, 37, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 277, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1243) [M::mem_pestat] mean and std.dev: (271.47, 150.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.34, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (370, 2614, 4616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13108) [M::mem_pestat] mean and std.dev: (3103.65, 2698.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17354) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (343, 763, 1388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3478) [M::mem_pestat] mean and std.dev: (698.19, 503.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4523) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1281.461 CPU sec, 74.451 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 937887, 38, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 326, 756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1876) [M::mem_pestat] mean and std.dev: (265.00, 159.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.45, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 192, 2887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8601) [M::mem_pestat] mean and std.dev: (1391.92, 1939.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11458) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (350, 704, 2020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5360) [M::mem_pestat] mean and std.dev: (1072.88, 1026.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7030) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1269.392 CPU sec, 55.897 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 939044, 38, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 446, 856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2150) [M::mem_pestat] mean and std.dev: (415.00, 276.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2797) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.45, 84.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 64, 1736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5146) [M::mem_pestat] mean and std.dev: (751.25, 1237.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6851) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (452, 1148, 2206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5714) [M::mem_pestat] mean and std.dev: (1253.62, 823.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7468) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1270.900 CPU sec, 66.859 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938772, 43, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 308, 650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1478) [M::mem_pestat] mean and std.dev: (418.06, 312.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.47, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (49, 1958, 4414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13144) [M::mem_pestat] mean and std.dev: (2565.21, 2785.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17509) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2400000 reads in 1309.309 CPU sec, 79.916 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938706, 34, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 287, 360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (255.88, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (109, 1424, 3026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8860) [M::mem_pestat] mean and std.dev: (2112.38, 2518.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12188) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (367, 666, 986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2224) [M::mem_pestat] mean and std.dev: (725.87, 462.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2843) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1260.735 CPU sec, 55.656 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 939012, 38, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 393, 958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2344) [M::mem_pestat] mean and std.dev: (539.67, 474.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3037) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 317, 2734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8094) [M::mem_pestat] mean and std.dev: (1463.03, 1997.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10774) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (301, 791, 1403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3607) [M::mem_pestat] mean and std.dev: (830.88, 610.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4709) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1291.957 CPU sec, 77.530 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 938765, 34, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (278, 325, 1811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4877) [M::mem_pestat] mean and std.dev: (1071.82, 1297.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.56, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (57, 738, 4432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13182) [M::mem_pestat] mean and std.dev: (2001.97, 2235.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17557) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 431, 1294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3538) [M::mem_pestat] mean and std.dev: (660.13, 679.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4660) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1259.207 CPU sec, 55.374 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938685, 38, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 419, 1124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2744) [M::mem_pestat] mean and std.dev: (799.32, 661.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.36, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 292, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7711) [M::mem_pestat] mean and std.dev: (1113.68, 1519.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10264) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (286, 1019, 3188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8992) [M::mem_pestat] mean and std.dev: (1809.05, 1971.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11894) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1283.721 CPU sec, 77.900 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938297, 33, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 374, 758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1746) [M::mem_pestat] mean and std.dev: (524.71, 370.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2240) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (49, 402, 3042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9028) [M::mem_pestat] mean and std.dev: (1658.55, 2009.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12021) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 645, 1039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2671) [M::mem_pestat] mean and std.dev: (599.11, 439.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3487) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1260.455 CPU sec, 55.431 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 938530, 26, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 350, 1150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3014) [M::mem_pestat] mean and std.dev: (561.89, 502.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3946) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.47, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (75, 183, 1678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4884) [M::mem_pestat] mean and std.dev: (1040.27, 1444.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6818) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2400000 reads in 1298.846 CPU sec, 70.856 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 938296, 30, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 251, 912) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2386) [M::mem_pestat] mean and std.dev: (284.46, 202.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3123) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 196, 2728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8100) [M::mem_pestat] mean and std.dev: (1322.76, 1643.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10786) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 875, 1120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2840) [M::mem_pestat] mean and std.dev: (750.45, 580.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3700) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1284.729 CPU sec, 74.751 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938033, 48, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 293, 730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1648) [M::mem_pestat] mean and std.dev: (368.12, 263.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2107) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 723, 3522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10458) [M::mem_pestat] mean and std.dev: (2022.73, 2592.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13926) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (278, 384, 1700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4544) [M::mem_pestat] mean and std.dev: (926.92, 1067.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5966) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1260.482 CPU sec, 55.584 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 939275, 36, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (266, 526, 1160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2948) [M::mem_pestat] mean and std.dev: (660.80, 506.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3842) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.36, 84.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (81, 560, 3085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9093) [M::mem_pestat] mean and std.dev: (2189.42, 3025.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14290) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (573, 811, 1358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2928) [M::mem_pestat] mean and std.dev: (769.69, 462.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3713) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1285.648 CPU sec, 76.269 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938040, 46, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 257, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1124) [M::mem_pestat] mean and std.dev: (247.31, 111.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.47, 84.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 346, 2996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8908) [M::mem_pestat] mean and std.dev: (1743.82, 2555.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11965) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2400000 reads in 1260.711 CPU sec, 55.481 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938357, 46, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 338, 938) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2386) [M::mem_pestat] mean and std.dev: (440.17, 330.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3110) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (53, 1681, 4444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13226) [M::mem_pestat] mean and std.dev: (2508.50, 2874.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17617) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 509, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1948) [M::mem_pestat] mean and std.dev: (445.87, 317.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2525) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1274.416 CPU sec, 75.176 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938657, 49, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 358, 1236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3110) [M::mem_pestat] mean and std.dev: (628.30, 647.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4047) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (109, 649) [M::mem_pestat] mean and std.dev: (380.17, 83.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (49, 251, 4439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13219) [M::mem_pestat] mean and std.dev: (1905.96, 2466.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17609) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (269, 382, 1107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2783) [M::mem_pestat] mean and std.dev: (634.12, 514.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3621) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1260.196 CPU sec, 55.490 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938786, 45, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (279, 471, 1053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2601) [M::mem_pestat] mean and std.dev: (661.80, 622.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (103, 821, 3511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10327) [M::mem_pestat] mean and std.dev: (1976.04, 2433.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13735) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (334, 757, 1215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2977) [M::mem_pestat] mean and std.dev: (797.05, 620.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3858) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1280.733 CPU sec, 74.829 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 938780, 49, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (115, 247, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1363) [M::mem_pestat] mean and std.dev: (320.17, 308.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1779) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.41, 84.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (84, 464, 3546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10470) [M::mem_pestat] mean and std.dev: (1803.78, 2301.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13932) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (243, 883, 1202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3120) [M::mem_pestat] mean and std.dev: (820.46, 721.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4079) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1282.137 CPU sec, 72.805 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 937740, 33, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 355, 2613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7229) [M::mem_pestat] mean and std.dev: (1498.56, 2009.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.47, 84.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 145, 1859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5557) [M::mem_pestat] mean and std.dev: (921.32, 1519.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7406) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (549, 1652, 3379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9039) [M::mem_pestat] mean and std.dev: (2296.60, 2451.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12103) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1261.660 CPU sec, 55.522 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938658, 40, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (315, 377, 1056) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2538) [M::mem_pestat] mean and std.dev: (444.89, 293.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3279) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.45, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (55, 1307, 3607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10711) [M::mem_pestat] mean and std.dev: (1986.50, 2404.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14263) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (202, 572, 1361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3679) [M::mem_pestat] mean and std.dev: (787.16, 752.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4838) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1297.766 CPU sec, 77.426 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938029, 41, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 338, 1041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2619) [M::mem_pestat] mean and std.dev: (605.11, 537.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3408) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.47, 84.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 163, 3635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10829) [M::mem_pestat] mean and std.dev: (1809.15, 2754.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14426) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (401, 548, 1412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3434) [M::mem_pestat] mean and std.dev: (646.38, 393.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4445) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1265.812 CPU sec, 55.673 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 937292, 35, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 357, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 928) [M::mem_pestat] mean and std.dev: (325.41, 153.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.34, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 184, 2591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7713) [M::mem_pestat] mean and std.dev: (792.06, 1358.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10274) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 760, 1911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5231) [M::mem_pestat] mean and std.dev: (999.08, 954.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6891) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1272.413 CPU sec, 76.035 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 938231, 32, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 306, 3750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10810) [M::mem_pestat] mean and std.dev: (1292.59, 1777.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14340) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.34, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (79, 922, 2615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7687) [M::mem_pestat] mean and std.dev: (1211.52, 1494.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10223) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (288, 374, 1221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3087) [M::mem_pestat] mean and std.dev: (699.08, 795.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4020) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1258.979 CPU sec, 55.366 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 939193, 37, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (272, 358, 922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2222) [M::mem_pestat] mean and std.dev: (503.00, 405.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2872) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.52, 84.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (69, 848, 2658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7836) [M::mem_pestat] mean and std.dev: (1570.19, 1780.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10425) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (342, 982, 3790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10686) [M::mem_pestat] mean and std.dev: (1771.54, 1841.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14134) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1293.101 CPU sec, 77.997 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938633, 40, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 340, 558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1282) [M::mem_pestat] mean and std.dev: (336.47, 207.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1644) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.32, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 253, 2851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8471) [M::mem_pestat] mean and std.dev: (1081.53, 1645.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11281) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 855, 2405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6755) [M::mem_pestat] mean and std.dev: (1214.36, 1255.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8930) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1270.654 CPU sec, 67.617 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 938400, 33, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (172, 340, 850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2206) [M::mem_pestat] mean and std.dev: (446.52, 406.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2884) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.40, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 452, 2627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7797) [M::mem_pestat] mean and std.dev: (1145.68, 1683.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10382) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 352, 1088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2794) [M::mem_pestat] mean and std.dev: (632.00, 591.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3647) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1259.552 CPU sec, 55.417 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 938876, 34, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 335, 830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2028) [M::mem_pestat] mean and std.dev: (479.07, 384.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.34, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (92, 301, 3107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9137) [M::mem_pestat] mean and std.dev: (1699.35, 2111.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12152) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 730, 2040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5724) [M::mem_pestat] mean and std.dev: (779.33, 750.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7566) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1298.284 CPU sec, 76.385 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 938377, 23, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (291, 373, 499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (364.85, 168.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1123) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.33, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (79, 1345, 2733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8041) [M::mem_pestat] mean and std.dev: (1684.17, 1766.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10695) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (278, 336, 1409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3671) [M::mem_pestat] mean and std.dev: (759.58, 661.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4802) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1248.120 CPU sec, 54.936 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 939098, 23, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 232, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1166) [M::mem_pestat] mean and std.dev: (326.18, 209.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.46, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (138, 667, 3692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10800) [M::mem_pestat] mean and std.dev: (2149.35, 2525.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14354) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 851, 1786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4844) [M::mem_pestat] mean and std.dev: (830.13, 701.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6373) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1275.743 CPU sec, 76.262 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 938515, 30, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 371, 1582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4332) [M::mem_pestat] mean and std.dev: (842.14, 927.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.28, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 125, 2182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6466) [M::mem_pestat] mean and std.dev: (925.69, 1520.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8608) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (296, 402, 3088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8672) [M::mem_pestat] mean and std.dev: (1547.36, 2102.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11464) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1263.333 CPU sec, 55.569 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938014, 29, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (307, 687, 1503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3895) [M::mem_pestat] mean and std.dev: (706.53, 606.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5091) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 1227, 2818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8362) [M::mem_pestat] mean and std.dev: (1487.82, 1610.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11134) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (350, 370, 1027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2381) [M::mem_pestat] mean and std.dev: (671.73, 537.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3058) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1290.726 CPU sec, 78.157 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 938211, 41, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 463, 1425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3627) [M::mem_pestat] mean and std.dev: (591.50, 448.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4728) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.48, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (58, 1127, 2647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7825) [M::mem_pestat] mean and std.dev: (1642.20, 1736.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10414) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (264, 406, 785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1827) [M::mem_pestat] mean and std.dev: (512.00, 345.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2348) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1265.368 CPU sec, 55.648 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 937504, 24, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 362, 720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1746) [M::mem_pestat] mean and std.dev: (465.70, 430.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2259) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.41, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (228, 1129, 3650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10494) [M::mem_pestat] mean and std.dev: (1905.29, 1947.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13916) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (206, 587, 1240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3308) [M::mem_pestat] mean and std.dev: (643.58, 491.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4342) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1316.378 CPU sec, 77.022 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 937644, 37, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (352, 721, 1889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4963) [M::mem_pestat] mean and std.dev: (1247.87, 1191.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.47, 84.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 342, 3803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11301) [M::mem_pestat] mean and std.dev: (2156.32, 2971.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15050) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1062, 1514, 2479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5313) [M::mem_pestat] mean and std.dev: (1488.00, 1042.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6730) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1275.086 CPU sec, 57.903 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 938268, 46, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (291, 348, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2061) [M::mem_pestat] mean and std.dev: (454.81, 345.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (69, 730, 2724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8034) [M::mem_pestat] mean and std.dev: (1297.88, 1586.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10689) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 655, 1238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3334) [M::mem_pestat] mean and std.dev: (742.94, 629.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4382) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1275.930 CPU sec, 71.303 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 938655, 34, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 277, 1205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3181) [M::mem_pestat] mean and std.dev: (657.00, 746.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4169) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.40, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 230, 1345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3949) [M::mem_pestat] mean and std.dev: (485.33, 786.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5251) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (360, 853, 1131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2673) [M::mem_pestat] mean and std.dev: (694.08, 438.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3444) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1271.102 CPU sec, 56.158 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938061, 32, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (260, 370, 1360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3560) [M::mem_pestat] mean and std.dev: (647.41, 598.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4660) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (51, 219, 2140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6318) [M::mem_pestat] mean and std.dev: (1107.03, 1526.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8407) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (298, 780, 1811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4837) [M::mem_pestat] mean and std.dev: (875.71, 899.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6350) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1282.148 CPU sec, 76.154 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 938925, 37, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (311, 397, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1094) [M::mem_pestat] mean and std.dev: (396.53, 205.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 904, 4483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13341) [M::mem_pestat] mean and std.dev: (2677.57, 3206.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17770) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (205, 792, 1051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2743) [M::mem_pestat] mean and std.dev: (741.59, 612.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3589) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1261.955 CPU sec, 55.501 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 938479, 33, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 430, 887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2131) [M::mem_pestat] mean and std.dev: (458.29, 280.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.32, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (65, 883, 3615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10715) [M::mem_pestat] mean and std.dev: (2250.73, 2848.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14265) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (371, 993, 1654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4220) [M::mem_pestat] mean and std.dev: (928.06, 716.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5503) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1267.411 CPU sec, 60.277 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 938311, 31, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 320, 793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2005) [M::mem_pestat] mean and std.dev: (405.18, 330.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.36, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (211, 885, 4156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12046) [M::mem_pestat] mean and std.dev: (2286.87, 2867.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15991) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2400000 reads in 1303.503 CPU sec, 78.518 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 937886, 40, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 309, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1009) [M::mem_pestat] mean and std.dev: (243.94, 142.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1303) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 125, 2692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8030) [M::mem_pestat] mean and std.dev: (1191.46, 1612.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10699) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (276, 334, 1713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4587) [M::mem_pestat] mean and std.dev: (825.27, 1067.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6024) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1267.443 CPU sec, 56.233 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 938579, 35, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 281, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 855) [M::mem_pestat] mean and std.dev: (245.65, 112.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1073) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.33, 84.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (102, 953, 3137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9207) [M::mem_pestat] mean and std.dev: (1880.77, 2135.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12242) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (318, 600, 1481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3807) [M::mem_pestat] mean and std.dev: (783.20, 740.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4970) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1280.548 CPU sec, 75.883 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 938899, 36, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 285, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1548) [M::mem_pestat] mean and std.dev: (377.84, 282.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1998) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.40, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 1193, 2652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7870) [M::mem_pestat] mean and std.dev: (1565.00, 1854.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10479) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 938, 1550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4162) [M::mem_pestat] mean and std.dev: (768.60, 580.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5468) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1262.895 CPU sec, 55.613 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 938145, 45, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 464, 2096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5638) [M::mem_pestat] mean and std.dev: (932.04, 962.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.33, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (60, 126, 2611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7713) [M::mem_pestat] mean and std.dev: (1310.74, 2038.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10264) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 469, 1096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2784) [M::mem_pestat] mean and std.dev: (613.20, 567.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3628) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1296.215 CPU sec, 77.180 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938132, 38, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (268, 339, 671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1477) [M::mem_pestat] mean and std.dev: (391.39, 256.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1880) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.47, 84.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 467, 2738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8122) [M::mem_pestat] mean and std.dev: (1633.39, 2083.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10814) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 655, 923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2313) [M::mem_pestat] mean and std.dev: (663.71, 550.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3008) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1260.302 CPU sec, 55.479 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 938473, 44, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 327, 520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1078) [M::mem_pestat] mean and std.dev: (365.46, 189.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.45, 84.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (82, 945, 3549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10483) [M::mem_pestat] mean and std.dev: (2039.86, 2444.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13950) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (548, 836, 2043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5033) [M::mem_pestat] mean and std.dev: (1182.08, 1145.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6528) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1277.705 CPU sec, 65.245 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938701, 30, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 359, 825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2005) [M::mem_pestat] mean and std.dev: (536.86, 466.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (51, 366, 2587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7659) [M::mem_pestat] mean and std.dev: (1159.07, 1523.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10195) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 487, 1394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3708) [M::mem_pestat] mean and std.dev: (641.67, 528.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4865) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1302.896 CPU sec, 78.885 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 938479, 38, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 317, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 835) [M::mem_pestat] mean and std.dev: (269.85, 99.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (326, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (110, 650) [M::mem_pestat] mean and std.dev: (380.49, 83.93) [M::mem_pestat] low and high boundaries for proper pairs: (2, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (59, 1332, 3993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11861) [M::mem_pestat] mean and std.dev: (1861.26, 2083.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15795) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2400000 reads in 1251.416 CPU sec, 55.150 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938954, 34, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 338, 847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2123) [M::mem_pestat] mean and std.dev: (497.70, 443.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (325, 1993, 3131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8743) [M::mem_pestat] mean and std.dev: (2437.76, 2510.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12478) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (383, 788, 1043) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2363) [M::mem_pestat] mean and std.dev: (591.80, 295.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3023) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1282.070 CPU sec, 75.128 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 938859, 38, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 326, 1394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3754) [M::mem_pestat] mean and std.dev: (574.82, 676.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4934) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.31, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (65, 1273, 2619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7727) [M::mem_pestat] mean and std.dev: (1473.29, 1619.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10281) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (264, 552, 907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2193) [M::mem_pestat] mean and std.dev: (609.27, 471.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2836) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1252.659 CPU sec, 55.076 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 938650, 33, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (246, 329, 1328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3492) [M::mem_pestat] mean and std.dev: (709.00, 726.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (326, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (110, 650) [M::mem_pestat] mean and std.dev: (380.59, 84.01) [M::mem_pestat] low and high boundaries for proper pairs: (2, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 97, 1218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3574) [M::mem_pestat] mean and std.dev: (439.47, 787.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4752) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (689, 1054, 1775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3947) [M::mem_pestat] mean and std.dev: (980.27, 496.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5033) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1284.876 CPU sec, 77.125 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 937975, 37, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 595, 1149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2921) [M::mem_pestat] mean and std.dev: (722.48, 638.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.36, 84.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (99, 300, 4826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14280) [M::mem_pestat] mean and std.dev: (2353.57, 3203.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19007) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (348, 540, 1502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3810) [M::mem_pestat] mean and std.dev: (930.25, 864.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4964) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1270.293 CPU sec, 55.961 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938451, 39, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 410, 848) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2228) [M::mem_pestat] mean and std.dev: (639.00, 620.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3121) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (326, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (110, 650) [M::mem_pestat] mean and std.dev: (380.51, 83.98) [M::mem_pestat] low and high boundaries for proper pairs: (2, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 1049, 4443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13217) [M::mem_pestat] mean and std.dev: (2135.41, 2777.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17604) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (597, 986, 1591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3579) [M::mem_pestat] mean and std.dev: (1014.44, 606.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4573) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1272.403 CPU sec, 67.817 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938082, 38, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 313, 1165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2971) [M::mem_pestat] mean and std.dev: (397.20, 314.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3874) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (81, 1514, 3668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10842) [M::mem_pestat] mean and std.dev: (2262.05, 2671.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14429) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 1059, 1878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5186) [M::mem_pestat] mean and std.dev: (998.09, 947.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6840) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1295.332 CPU sec, 77.756 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938138, 31, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (355, 752, 1117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2641) [M::mem_pestat] mean and std.dev: (741.68, 518.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3403) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (68, 592, 4425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13139) [M::mem_pestat] mean and std.dev: (2108.06, 2766.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17496) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (307, 626, 1355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3451) [M::mem_pestat] mean and std.dev: (837.53, 801.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4499) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1263.913 CPU sec, 55.764 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 937892, 37, 21) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.28, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (68, 269, 1394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4046) [M::mem_pestat] mean and std.dev: (842.64, 1053.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5372) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 695, 1905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5231) [M::mem_pestat] mean and std.dev: (791.00, 721.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6894) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1267.347 CPU sec, 74.287 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938403, 29, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (269, 333, 1555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4127) [M::mem_pestat] mean and std.dev: (661.06, 658.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (97, 475, 3098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9100) [M::mem_pestat] mean and std.dev: (1627.00, 2297.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12101) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (430, 934, 1271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2953) [M::mem_pestat] mean and std.dev: (780.64, 524.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3794) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1262.063 CPU sec, 55.659 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 938394, 38, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 312, 1559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4177) [M::mem_pestat] mean and std.dev: (637.14, 734.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.46, 84.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 1924, 2960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8786) [M::mem_pestat] mean and std.dev: (2123.39, 2122.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11699) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 279, 1476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3966) [M::mem_pestat] mean and std.dev: (596.50, 621.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5211) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1279.496 CPU sec, 74.124 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938941, 32, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (110, 295, 877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2411) [M::mem_pestat] mean and std.dev: (480.71, 546.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3178) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.51, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (59, 698, 3149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9329) [M::mem_pestat] mean and std.dev: (1865.72, 2342.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12419) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (256, 489, 984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2440) [M::mem_pestat] mean and std.dev: (609.95, 531.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3168) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1251.650 CPU sec, 55.101 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938510, 33, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (266, 326, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1355) [M::mem_pestat] mean and std.dev: (417.95, 214.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (70, 1195, 2921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8623) [M::mem_pestat] mean and std.dev: (1653.85, 1891.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11474) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (324, 800, 1521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3915) [M::mem_pestat] mean and std.dev: (895.47, 687.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5112) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1268.636 CPU sec, 71.862 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 937170, 35, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (390, 898, 1607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4041) [M::mem_pestat] mean and std.dev: (858.62, 685.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5258) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.45, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 138, 1300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3810) [M::mem_pestat] mean and std.dev: (477.17, 752.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5065) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (301, 491, 2176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5926) [M::mem_pestat] mean and std.dev: (1052.17, 1421.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7801) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1265.523 CPU sec, 71.886 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938141, 29, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 356, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2464) [M::mem_pestat] mean and std.dev: (622.85, 546.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3195) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (326, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (110, 650) [M::mem_pestat] mean and std.dev: (380.42, 84.06) [M::mem_pestat] low and high boundaries for proper pairs: (2, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 292, 3109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9227) [M::mem_pestat] mean and std.dev: (1973.86, 2683.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12710) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 681, 1345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3521) [M::mem_pestat] mean and std.dev: (839.92, 757.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4609) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1252.761 CPU sec, 55.054 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 938438, 39, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 346, 1022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2664) [M::mem_pestat] mean and std.dev: (504.67, 403.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.52, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (60, 664, 3553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10539) [M::mem_pestat] mean and std.dev: (1840.97, 2189.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14032) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 393, 1815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4931) [M::mem_pestat] mean and std.dev: (885.17, 1092.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6489) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1275.579 CPU sec, 73.772 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 939018, 34, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (280, 339, 1028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2524) [M::mem_pestat] mean and std.dev: (523.22, 408.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3272) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.41, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (78, 1498, 3096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9132) [M::mem_pestat] mean and std.dev: (1980.44, 2318.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12150) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (262, 1041, 2074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5698) [M::mem_pestat] mean and std.dev: (1133.43, 832.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7510) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1255.504 CPU sec, 55.188 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 937513, 43, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (285, 326, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1218) [M::mem_pestat] mean and std.dev: (376.17, 204.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.36, 84.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 302, 2694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8000) [M::mem_pestat] mean and std.dev: (1245.20, 1700.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10653) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 1065, 1735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4781) [M::mem_pestat] mean and std.dev: (1095.18, 972.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6304) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1292.592 CPU sec, 75.746 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938037, 31, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 741, 1318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3370) [M::mem_pestat] mean and std.dev: (737.90, 479.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4396) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (65, 267, 3714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11012) [M::mem_pestat] mean and std.dev: (2046.29, 2915.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14661) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (374, 748, 2493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6731) [M::mem_pestat] mean and std.dev: (1567.07, 1891.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9134) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1254.322 CPU sec, 55.124 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938142, 31, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 373, 764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1750) [M::mem_pestat] mean and std.dev: (417.40, 260.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2243) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.48, 84.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 332, 4472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13326) [M::mem_pestat] mean and std.dev: (2541.48, 2950.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17753) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (282, 782, 1409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3663) [M::mem_pestat] mean and std.dev: (796.64, 829.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4790) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1278.709 CPU sec, 76.590 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 937652, 29, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (239, 354, 1132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2918) [M::mem_pestat] mean and std.dev: (542.20, 466.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.38, 84.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (95, 1294, 4412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13046) [M::mem_pestat] mean and std.dev: (2389.45, 2516.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17363) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (303, 991, 1381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3537) [M::mem_pestat] mean and std.dev: (970.92, 805.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4615) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1248.609 CPU sec, 58.172 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 939579, 30, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 295, 688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1656) [M::mem_pestat] mean and std.dev: (385.46, 366.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2140) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.49, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (79, 1097, 4438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13156) [M::mem_pestat] mean and std.dev: (2287.13, 2704.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17515) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (200, 738, 1405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3815) [M::mem_pestat] mean and std.dev: (650.00, 694.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5020) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1249.611 CPU sec, 55.048 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 938012, 37, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 292, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (295.70, 114.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.52, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (70, 426, 2856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8428) [M::mem_pestat] mean and std.dev: (1306.03, 1579.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11214) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 616, 1062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2676) [M::mem_pestat] mean and std.dev: (692.32, 592.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3483) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1275.163 CPU sec, 74.436 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 939131, 27, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (246, 421, 869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2115) [M::mem_pestat] mean and std.dev: (523.61, 439.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.48, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (73, 230, 4457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13225) [M::mem_pestat] mean and std.dev: (1858.26, 2352.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17609) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (415, 759, 1879) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4807) [M::mem_pestat] mean and std.dev: (1089.95, 851.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6271) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1248.485 CPU sec, 54.919 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938806, 31, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 321, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 966) [M::mem_pestat] mean and std.dev: (288.89, 163.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1222) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.19, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 287, 2569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7595) [M::mem_pestat] mean and std.dev: (1192.74, 1492.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10108) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 682, 1690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4616) [M::mem_pestat] mean and std.dev: (606.94, 515.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6079) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1268.523 CPU sec, 72.937 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938694, 38, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (239, 627, 1076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2750) [M::mem_pestat] mean and std.dev: (732.00, 603.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (74, 2569, 4065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12047) [M::mem_pestat] mean and std.dev: (2406.37, 2485.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16038) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (269, 482, 1613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4301) [M::mem_pestat] mean and std.dev: (902.12, 835.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5645) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1261.014 CPU sec, 55.438 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 938224, 41, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 333, 611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1279) [M::mem_pestat] mean and std.dev: (347.90, 250.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.47, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 188, 2744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8144) [M::mem_pestat] mean and std.dev: (1121.97, 1470.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10844) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 778, 1871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5125) [M::mem_pestat] mean and std.dev: (1012.25, 1198.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6752) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1279.089 CPU sec, 75.989 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 937876, 28, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 469, 1634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4528) [M::mem_pestat] mean and std.dev: (689.41, 684.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5975) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.62, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (108, 1091, 3070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8994) [M::mem_pestat] mean and std.dev: (1897.71, 2315.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11956) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (241, 656, 1137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2929) [M::mem_pestat] mean and std.dev: (772.73, 584.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3825) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1259.859 CPU sec, 66.604 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 938751, 26, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (167, 295, 722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1832) [M::mem_pestat] mean and std.dev: (505.88, 525.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.30, 84.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 252, 2763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8235) [M::mem_pestat] mean and std.dev: (1445.58, 1790.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10971) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (281, 385, 1768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4742) [M::mem_pestat] mean and std.dev: (882.71, 834.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6229) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1250.116 CPU sec, 55.007 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 938541, 43, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 407, 899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2235) [M::mem_pestat] mean and std.dev: (425.83, 362.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2903) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.52, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 117, 3638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10834) [M::mem_pestat] mean and std.dev: (1693.12, 2583.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14432) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 1044, 1447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3867) [M::mem_pestat] mean and std.dev: (1110.71, 781.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5077) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1269.921 CPU sec, 70.713 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938713, 38, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (246, 420, 1257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3279) [M::mem_pestat] mean and std.dev: (616.86, 562.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4290) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.40, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (67, 1913, 3502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10372) [M::mem_pestat] mean and std.dev: (2226.63, 2628.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13807) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 534, 793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1949) [M::mem_pestat] mean and std.dev: (517.67, 437.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1254.841 CPU sec, 55.187 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 939198, 36, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (258, 489, 1491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3957) [M::mem_pestat] mean and std.dev: (958.12, 972.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5190) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 145, 2471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7313) [M::mem_pestat] mean and std.dev: (1131.00, 1784.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9734) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (521, 811, 1042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2084) [M::mem_pestat] mean and std.dev: (772.29, 395.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2605) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1268.617 CPU sec, 72.506 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 938639, 39, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (300, 514, 1062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2586) [M::mem_pestat] mean and std.dev: (627.04, 478.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3348) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.36, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 179, 1989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5867) [M::mem_pestat] mean and std.dev: (979.76, 1317.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7806) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 285, 1337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3549) [M::mem_pestat] mean and std.dev: (489.50, 403.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4655) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1259.387 CPU sec, 55.384 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 937735, 31, 17) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.33, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (55, 223, 2618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7744) [M::mem_pestat] mean and std.dev: (1186.76, 1498.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10307) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (282, 925, 1489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3903) [M::mem_pestat] mean and std.dev: (839.73, 779.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5110) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1277.591 CPU sec, 74.410 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938408, 38, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (268, 323, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (300.00, 97.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (66, 1840, 3302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9774) [M::mem_pestat] mean and std.dev: (2253.42, 2526.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13010) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (303, 988, 1775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4719) [M::mem_pestat] mean and std.dev: (1106.00, 1111.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1248.051 CPU sec, 54.863 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 937705, 30, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (308, 419, 683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1433) [M::mem_pestat] mean and std.dev: (404.76, 279.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.54, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 215, 1348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3954) [M::mem_pestat] mean and std.dev: (699.82, 987.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5257) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2400000 reads in 1290.253 CPU sec, 76.051 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 938206, 41, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 268, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (315.74, 235.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.33, 84.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (110, 1695, 4429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13067) [M::mem_pestat] mean and std.dev: (2531.07, 2621.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17386) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2400000 reads in 1248.372 CPU sec, 56.643 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 938971, 34, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (257, 412, 1094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2768) [M::mem_pestat] mean and std.dev: (640.03, 536.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.45, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (64, 905, 4420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13132) [M::mem_pestat] mean and std.dev: (2242.38, 2730.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17488) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (234, 709, 1695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4617) [M::mem_pestat] mean and std.dev: (624.27, 487.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6078) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1265.837 CPU sec, 72.638 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 937828, 36, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 389, 1076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2738) [M::mem_pestat] mean and std.dev: (591.21, 542.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 122, 2014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6008) [M::mem_pestat] mean and std.dev: (899.74, 1283.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8005) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (274, 954, 1469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3859) [M::mem_pestat] mean and std.dev: (1024.19, 917.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5054) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1249.735 CPU sec, 55.131 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938695, 30, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (293, 421, 984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2366) [M::mem_pestat] mean and std.dev: (550.85, 452.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3057) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.40, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (181, 2581, 3678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10672) [M::mem_pestat] mean and std.dev: (2106.37, 1699.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14169) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 369, 893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2255) [M::mem_pestat] mean and std.dev: (511.10, 391.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2936) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1268.157 CPU sec, 73.264 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 938642, 41, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (270, 658, 2076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5688) [M::mem_pestat] mean and std.dev: (672.00, 560.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.50, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 191, 2894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8636) [M::mem_pestat] mean and std.dev: (1600.41, 2197.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11507) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (289, 798, 1441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3745) [M::mem_pestat] mean and std.dev: (845.18, 658.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4897) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1243.446 CPU sec, 54.782 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 939312, 20, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 423, 1227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3253) [M::mem_pestat] mean and std.dev: (483.79, 456.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4266) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.30, 84.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (63, 2665, 4454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13236) [M::mem_pestat] mean and std.dev: (2348.60, 2210.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17627) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (430, 823, 3844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10672) [M::mem_pestat] mean and std.dev: (1770.00, 1869.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14086) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1244.810 CPU sec, 60.214 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938933, 42, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 343, 944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2328) [M::mem_pestat] mean and std.dev: (560.42, 452.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3020) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.28, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 1782, 3310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9868) [M::mem_pestat] mean and std.dev: (2077.43, 2307.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13147) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (173, 281, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1211) [M::mem_pestat] mean and std.dev: (260.67, 161.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1557) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1277.512 CPU sec, 77.087 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 938343, 44, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 346, 1161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2899) [M::mem_pestat] mean and std.dev: (598.36, 663.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.51, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (71, 1382, 3063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9047) [M::mem_pestat] mean and std.dev: (2036.43, 2369.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12039) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (327, 787, 2588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7110) [M::mem_pestat] mean and std.dev: (1324.86, 1400.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9371) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1239.264 CPU sec, 55.476 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 938930, 42, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (310, 909, 1245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3115) [M::mem_pestat] mean and std.dev: (790.00, 516.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4050) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.33, 84.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (73, 270, 2589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7621) [M::mem_pestat] mean and std.dev: (1018.85, 1243.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10137) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (262, 582, 1051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2629) [M::mem_pestat] mean and std.dev: (619.50, 561.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3418) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1258.340 CPU sec, 72.218 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 938440, 23, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 318, 964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2396) [M::mem_pestat] mean and std.dev: (520.83, 531.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3112) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.41, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 156, 2863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8485) [M::mem_pestat] mean and std.dev: (1724.45, 2385.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11296) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (337, 905, 1324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3298) [M::mem_pestat] mean and std.dev: (872.28, 639.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4285) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1241.408 CPU sec, 54.769 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938661, 45, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (239, 333, 940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2342) [M::mem_pestat] mean and std.dev: (635.11, 637.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3184) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.40, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 1123, 4516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13452) [M::mem_pestat] mean and std.dev: (2217.89, 2730.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17920) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (520, 670, 4642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12886) [M::mem_pestat] mean and std.dev: (1907.83, 2477.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17008) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1261.769 CPU sec, 73.601 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 938649, 42, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (134, 294, 728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1916) [M::mem_pestat] mean and std.dev: (332.33, 294.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.30, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (87, 1462, 2680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7866) [M::mem_pestat] mean and std.dev: (1452.55, 1526.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10459) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (276, 520, 916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2196) [M::mem_pestat] mean and std.dev: (619.58, 511.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2836) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1248.945 CPU sec, 55.018 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 939442, 27, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 306, 728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1730) [M::mem_pestat] mean and std.dev: (448.45, 369.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.45, 84.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (93, 303, 1883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5463) [M::mem_pestat] mean and std.dev: (1139.54, 1462.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7253) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (265, 625, 1375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3595) [M::mem_pestat] mean and std.dev: (858.92, 742.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4705) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1248.970 CPU sec, 64.677 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 938845, 39, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (249, 348, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (272.00, 97.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 362, 2683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7955) [M::mem_pestat] mean and std.dev: (1148.20, 1531.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10591) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 315, 1743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4841) [M::mem_pestat] mean and std.dev: (813.13, 1128.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6390) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1265.661 CPU sec, 76.711 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938790, 41, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 422, 1452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3702) [M::mem_pestat] mean and std.dev: (726.95, 698.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.36, 84.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 157, 2694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7994) [M::mem_pestat] mean and std.dev: (1141.20, 1390.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10644) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (196, 331, 756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1876) [M::mem_pestat] mean and std.dev: (398.87, 268.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2436) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1233.467 CPU sec, 54.486 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 938299, 44, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (311, 580, 1168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2882) [M::mem_pestat] mean and std.dev: (809.05, 657.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.33, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (53, 589, 3319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9851) [M::mem_pestat] mean and std.dev: (1942.59, 2424.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13117) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 449, 1054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2790) [M::mem_pestat] mean and std.dev: (395.12, 288.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3658) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1266.766 CPU sec, 72.457 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 937517, 35, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 355, 713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1785) [M::mem_pestat] mean and std.dev: (411.04, 347.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.46, 84.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 106, 3387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10087) [M::mem_pestat] mean and std.dev: (1600.37, 2308.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13437) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 470, 1371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3661) [M::mem_pestat] mean and std.dev: (591.15, 532.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4806) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1249.003 CPU sec, 54.990 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 938669, 42, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 273, 912) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2344) [M::mem_pestat] mean and std.dev: (503.96, 460.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3060) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.35, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 310, 3696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10994) [M::mem_pestat] mean and std.dev: (1961.71, 2726.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14643) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (429, 1009, 1293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3021) [M::mem_pestat] mean and std.dev: (865.88, 435.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3885) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1258.787 CPU sec, 73.704 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 939064, 42, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 381, 1472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3910) [M::mem_pestat] mean and std.dev: (519.00, 489.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5129) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.41, 84.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 644, 2599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7707) [M::mem_pestat] mean and std.dev: (1141.25, 1334.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10261) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (352, 849, 1141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2719) [M::mem_pestat] mean and std.dev: (806.31, 583.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3508) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.566 CPU sec, 54.271 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938265, 45, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (257, 452, 906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2204) [M::mem_pestat] mean and std.dev: (570.57, 468.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (61, 934, 3110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9208) [M::mem_pestat] mean and std.dev: (1771.12, 2141.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12257) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (834, 1051, 1629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3219) [M::mem_pestat] mean and std.dev: (1057.82, 604.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4014) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1258.428 CPU sec, 68.409 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938454, 34, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 335, 651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1585) [M::mem_pestat] mean and std.dev: (400.00, 336.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2052) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.48, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (68, 213, 2733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8063) [M::mem_pestat] mean and std.dev: (1486.84, 2232.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10728) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 411, 1242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3286) [M::mem_pestat] mean and std.dev: (628.36, 621.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4308) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1258.253 CPU sec, 72.769 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 939278, 39, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 383, 659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1429) [M::mem_pestat] mean and std.dev: (431.80, 280.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1814) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.35, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 214, 2527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7469) [M::mem_pestat] mean and std.dev: (1025.97, 1476.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9940) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (370, 1463, 2255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6025) [M::mem_pestat] mean and std.dev: (1764.86, 1627.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8277) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1234.819 CPU sec, 54.357 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 939225, 25, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 293, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1448) [M::mem_pestat] mean and std.dev: (292.76, 185.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1867) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.46, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (62, 403, 3618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10730) [M::mem_pestat] mean and std.dev: (1975.72, 2557.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14286) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (585, 1199, 2368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5934) [M::mem_pestat] mean and std.dev: (1212.00, 914.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7717) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1253.971 CPU sec, 73.336 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 938270, 34, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (269, 372, 1104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2774) [M::mem_pestat] mean and std.dev: (610.78, 505.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 83.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 366, 4436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13224) [M::mem_pestat] mean and std.dev: (2154.94, 2884.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17618) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 446, 1638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4538) [M::mem_pestat] mean and std.dev: (716.12, 697.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5988) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1238.745 CPU sec, 54.507 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 938963, 40, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (163, 302, 1300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3574) [M::mem_pestat] mean and std.dev: (687.43, 864.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.40, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (53, 706, 3785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11249) [M::mem_pestat] mean and std.dev: (1843.58, 2522.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14981) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (332, 437, 3680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10376) [M::mem_pestat] mean and std.dev: (1364.88, 1611.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13724) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1258.654 CPU sec, 73.101 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 937809, 40, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 578, 1183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2921) [M::mem_pestat] mean and std.dev: (659.32, 436.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3790) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.38, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (72, 1621, 3297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9747) [M::mem_pestat] mean and std.dev: (2554.72, 2998.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14548) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (558, 931, 1828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4368) [M::mem_pestat] mean and std.dev: (798.80, 463.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5638) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1240.898 CPU sec, 54.668 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938505, 42, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 293, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (250.42, 85.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.26, 84.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 222, 3572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10642) [M::mem_pestat] mean and std.dev: (1722.29, 2339.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14177) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (262, 446, 1110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2806) [M::mem_pestat] mean and std.dev: (431.11, 311.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3654) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1264.198 CPU sec, 72.803 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938656, 39, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (390, 728, 1129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2607) [M::mem_pestat] mean and std.dev: (810.00, 568.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3346) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (84, 329, 2339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6849) [M::mem_pestat] mean and std.dev: (1335.03, 1790.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9104) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (655, 996, 1632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3586) [M::mem_pestat] mean and std.dev: (1161.35, 736.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4563) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1233.195 CPU sec, 60.725 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938569, 35, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (272, 509, 1406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3674) [M::mem_pestat] mean and std.dev: (865.22, 861.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4808) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.47, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 88, 2860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8508) [M::mem_pestat] mean and std.dev: (1223.94, 1932.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11332) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (271, 663, 1390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3628) [M::mem_pestat] mean and std.dev: (714.95, 596.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4747) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1225.507 CPU sec, 53.822 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 938588, 41, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 315, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 756) [M::mem_pestat] mean and std.dev: (283.44, 140.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 940) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.30, 84.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (72, 536, 3858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11430) [M::mem_pestat] mean and std.dev: (1786.98, 2284.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15216) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1251.750 CPU sec, 73.384 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938660, 34, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 288, 750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1908) [M::mem_pestat] mean and std.dev: (443.09, 413.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (86, 477, 2752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8084) [M::mem_pestat] mean and std.dev: (1722.06, 2127.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10750) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (317, 902, 1273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3185) [M::mem_pestat] mean and std.dev: (702.00, 528.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4141) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1234.327 CPU sec, 54.370 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938499, 37, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 300, 498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1054) [M::mem_pestat] mean and std.dev: (267.19, 102.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1332) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.33, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (67, 382, 2852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8422) [M::mem_pestat] mean and std.dev: (1362.08, 1723.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11207) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (797, 1246, 1668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3410) [M::mem_pestat] mean and std.dev: (1000.13, 517.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4281) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1259.952 CPU sec, 73.405 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 938164, 45, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 339, 847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2075) [M::mem_pestat] mean and std.dev: (469.04, 410.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.31, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 81, 2615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7777) [M::mem_pestat] mean and std.dev: (1195.91, 1805.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10358) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (178, 268, 1335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3649) [M::mem_pestat] mean and std.dev: (694.27, 679.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4806) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1240.649 CPU sec, 54.595 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 937913, 37, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 318, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 960) [M::mem_pestat] mean and std.dev: (311.00, 169.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1210) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.32, 84.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (72, 743, 4000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11856) [M::mem_pestat] mean and std.dev: (2074.05, 2602.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15784) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 879, 1524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4108) [M::mem_pestat] mean and std.dev: (850.82, 786.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5400) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1260.411 CPU sec, 75.171 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938190, 34, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 273, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (230.94, 121.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 116, 3081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9187) [M::mem_pestat] mean and std.dev: (1862.71, 2740.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12824) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (352, 505, 1314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3238) [M::mem_pestat] mean and std.dev: (614.59, 393.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4200) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1256.709 CPU sec, 63.783 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 937414, 38, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 315, 1114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2918) [M::mem_pestat] mean and std.dev: (614.06, 702.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3820) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (104, 1346, 3601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10595) [M::mem_pestat] mean and std.dev: (2213.47, 2594.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14092) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 404, 3632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10422) [M::mem_pestat] mean and std.dev: (1804.54, 2456.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13817) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1245.108 CPU sec, 54.671 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 938283, 38, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 317, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1219) [M::mem_pestat] mean and std.dev: (341.55, 247.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.41, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (91, 863, 2601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7621) [M::mem_pestat] mean and std.dev: (1534.26, 1813.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10131) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (360, 1127, 1518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3834) [M::mem_pestat] mean and std.dev: (882.21, 613.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4992) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1265.931 CPU sec, 73.794 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 937859, 32, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 365, 954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2458) [M::mem_pestat] mean and std.dev: (487.31, 402.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3210) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 971, 2320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6852) [M::mem_pestat] mean and std.dev: (1251.67, 1559.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9118) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (297, 389, 1044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2538) [M::mem_pestat] mean and std.dev: (551.64, 409.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3285) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1236.867 CPU sec, 54.442 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 938121, 36, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 271, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 769) [M::mem_pestat] mean and std.dev: (264.25, 130.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.55, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 1921, 3310) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9858) [M::mem_pestat] mean and std.dev: (2077.33, 2523.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13132) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (309, 426, 1201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2985) [M::mem_pestat] mean and std.dev: (786.15, 663.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3877) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1270.203 CPU sec, 70.153 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938699, 34, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 313, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1072) [M::mem_pestat] mean and std.dev: (325.19, 202.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.50, 84.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 206, 2435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7233) [M::mem_pestat] mean and std.dev: (1153.48, 1510.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9632) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 633, 1445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3975) [M::mem_pestat] mean and std.dev: (836.33, 968.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5240) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.076 CPU sec, 54.304 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 938567, 34, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 333, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006) [M::mem_pestat] mean and std.dev: (366.57, 219.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1256) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (326, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (110, 650) [M::mem_pestat] mean and std.dev: (380.43, 83.98) [M::mem_pestat] low and high boundaries for proper pairs: (2, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 202, 2918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8678) [M::mem_pestat] mean and std.dev: (1362.38, 1868.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11558) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1261.073 CPU sec, 74.999 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 937919, 31, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 351, 970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2344) [M::mem_pestat] mean and std.dev: (401.73, 294.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3031) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.40, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 153, 4041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12069) [M::mem_pestat] mean and std.dev: (1926.77, 2616.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16083) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (277, 380, 1027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2527) [M::mem_pestat] mean and std.dev: (466.00, 329.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3277) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1239.891 CPU sec, 54.470 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 939166, 35, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (306, 418, 1903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5097) [M::mem_pestat] mean and std.dev: (949.13, 914.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.36, 84.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 469, 3019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8995) [M::mem_pestat] mean and std.dev: (1652.34, 2185.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11983) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (226, 752, 1587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4309) [M::mem_pestat] mean and std.dev: (925.81, 733.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5670) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1278.046 CPU sec, 75.443 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938447, 30, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 217, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (253.62, 123.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1165) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 180, 1249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3711) [M::mem_pestat] mean and std.dev: (372.36, 696.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4942) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (341, 673, 1503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3827) [M::mem_pestat] mean and std.dev: (694.09, 500.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4989) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1240.235 CPU sec, 56.461 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 938620, 26, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (261, 345, 937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2289) [M::mem_pestat] mean and std.dev: (498.90, 340.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.56, 84.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (119, 2583, 4484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13214) [M::mem_pestat] mean and std.dev: (2737.08, 3065.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17579) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (322, 556, 1432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3652) [M::mem_pestat] mean and std.dev: (764.87, 678.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4762) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1258.611 CPU sec, 71.179 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 939652, 45, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (243, 318, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 978) [M::mem_pestat] mean and std.dev: (357.38, 192.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1223) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (326, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (110, 650) [M::mem_pestat] mean and std.dev: (380.50, 84.04) [M::mem_pestat] low and high boundaries for proper pairs: (2, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 71, 1873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5547) [M::mem_pestat] mean and std.dev: (772.52, 1226.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7384) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (354, 565, 829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1779) [M::mem_pestat] mean and std.dev: (613.18, 385.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2254) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1237.346 CPU sec, 54.588 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 938506, 33, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 343, 1000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2470) [M::mem_pestat] mean and std.dev: (556.04, 512.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3205) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.58, 84.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (64, 393, 1769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5179) [M::mem_pestat] mean and std.dev: (964.50, 1376.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6884) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 771, 1130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2894) [M::mem_pestat] mean and std.dev: (799.29, 699.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3776) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1263.068 CPU sec, 73.197 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938659, 37, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 384, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1312) [M::mem_pestat] mean and std.dev: (470.67, 275.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1655) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.45, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (60, 1266, 3079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9117) [M::mem_pestat] mean and std.dev: (1856.73, 2185.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12136) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (289, 1163, 1705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4537) [M::mem_pestat] mean and std.dev: (1058.46, 939.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5953) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1241.454 CPU sec, 54.666 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 938852, 36, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 400, 1073) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2737) [M::mem_pestat] mean and std.dev: (505.30, 483.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.40, 84.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (57, 1211, 3623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10755) [M::mem_pestat] mean and std.dev: (2017.08, 2218.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14321) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (316, 739, 1442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3694) [M::mem_pestat] mean and std.dev: (842.64, 759.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4820) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1231.724 CPU sec, 57.198 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 938782, 42, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 340, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (289.64, 106.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1088) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.54, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (82, 916, 4419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13093) [M::mem_pestat] mean and std.dev: (2277.67, 2758.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17430) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 566, 1324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3494) [M::mem_pestat] mean and std.dev: (709.00, 541.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4579) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1292.313 CPU sec, 75.808 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 939394, 30, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (170, 273, 448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004) [M::mem_pestat] mean and std.dev: (275.78, 190.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1282) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.55, 84.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (53, 156, 2528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7478) [M::mem_pestat] mean and std.dev: (980.36, 1285.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9953) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (269, 340, 1192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3038) [M::mem_pestat] mean and std.dev: (496.25, 458.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3961) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1233.950 CPU sec, 55.894 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 938186, 43, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 443, 1631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4419) [M::mem_pestat] mean and std.dev: (966.47, 1079.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5813) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (69, 1323, 3094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9144) [M::mem_pestat] mean and std.dev: (1759.52, 1985.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12169) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (219, 910, 1933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5361) [M::mem_pestat] mean and std.dev: (739.30, 638.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7075) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1260.239 CPU sec, 72.595 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 939473, 30, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 365, 1425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3781) [M::mem_pestat] mean and std.dev: (770.72, 839.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4959) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.50, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (77, 732, 2629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7733) [M::mem_pestat] mean and std.dev: (1472.90, 1902.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10285) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 930, 1285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3323) [M::mem_pestat] mean and std.dev: (902.24, 870.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4385) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1247.573 CPU sec, 55.121 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938217, 34, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 301, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112) [M::mem_pestat] mean and std.dev: (331.89, 206.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1416) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.34, 84.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 187, 3044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9024) [M::mem_pestat] mean and std.dev: (1763.94, 2493.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12014) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (310, 728, 1713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4519) [M::mem_pestat] mean and std.dev: (899.42, 857.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5922) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1265.769 CPU sec, 75.449 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 938683, 34, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (194, 389, 951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2465) [M::mem_pestat] mean and std.dev: (429.83, 301.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3222) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.40, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (62, 675, 4464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13268) [M::mem_pestat] mean and std.dev: (2089.09, 2679.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17670) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (221, 437, 1357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3629) [M::mem_pestat] mean and std.dev: (681.38, 581.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4765) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1233.206 CPU sec, 54.287 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 938581, 35, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 305, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (302.25, 161.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1135) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.30, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (64, 1272, 4452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13228) [M::mem_pestat] mean and std.dev: (2099.91, 2371.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17616) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (389, 1015, 1352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3278) [M::mem_pestat] mean and std.dev: (926.67, 620.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4241) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1245.610 CPU sec, 62.060 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 937884, 34, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (303, 405, 1037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2505) [M::mem_pestat] mean and std.dev: (631.50, 574.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3239) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.30, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 139, 1512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4458) [M::mem_pestat] mean and std.dev: (784.00, 1131.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5931) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (339, 546, 1361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3405) [M::mem_pestat] mean and std.dev: (814.11, 915.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4478) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1255.730 CPU sec, 70.463 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 939227, 43, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 288, 1029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2611) [M::mem_pestat] mean and std.dev: (622.81, 619.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.47, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 744, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7758) [M::mem_pestat] mean and std.dev: (1397.71, 1599.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10334) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 319, 1158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3066) [M::mem_pestat] mean and std.dev: (587.76, 527.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4020) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.789 CPU sec, 54.491 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 938279, 43, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 290, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (222.91, 86.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 859) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.53, 84.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 159, 2486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7372) [M::mem_pestat] mean and std.dev: (1202.35, 1609.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9815) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (332, 596, 1241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3059) [M::mem_pestat] mean and std.dev: (739.89, 483.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3968) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1261.088 CPU sec, 73.424 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938823, 35, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 419, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1656) [M::mem_pestat] mean and std.dev: (464.06, 301.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2128) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.59, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 239, 2782) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8262) [M::mem_pestat] mean and std.dev: (1436.54, 1932.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11002) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (338, 799, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2663) [M::mem_pestat] mean and std.dev: (864.89, 684.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3602) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1241.860 CPU sec, 54.621 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 938029, 40, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 343, 1104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2800) [M::mem_pestat] mean and std.dev: (485.50, 514.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 281, 2535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7505) [M::mem_pestat] mean and std.dev: (1066.50, 1409.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9990) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (322, 473, 1046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2494) [M::mem_pestat] mean and std.dev: (797.05, 615.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3258) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1270.048 CPU sec, 75.650 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 938814, 28, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 243, 720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1752) [M::mem_pestat] mean and std.dev: (284.25, 176.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2268) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.34, 84.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (60, 560, 2378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7014) [M::mem_pestat] mean and std.dev: (1139.96, 1447.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9332) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 686, 1019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2583) [M::mem_pestat] mean and std.dev: (666.89, 486.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3365) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1237.971 CPU sec, 54.447 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938671, 44, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 326, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (322.41, 178.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 272, 2924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8694) [M::mem_pestat] mean and std.dev: (1581.60, 2452.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11579) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2400000 reads in 1252.510 CPU sec, 66.364 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938479, 39, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 393, 787) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1919) [M::mem_pestat] mean and std.dev: (485.82, 401.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.35, 84.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (61, 227, 2751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8131) [M::mem_pestat] mean and std.dev: (1251.73, 1820.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10821) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 721, 1145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3005) [M::mem_pestat] mean and std.dev: (748.67, 588.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3935) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1264.141 CPU sec, 73.421 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 938669, 41, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 278, 727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1759) [M::mem_pestat] mean and std.dev: (390.75, 362.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2275) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (79, 783, 2610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7672) [M::mem_pestat] mean and std.dev: (1347.30, 1772.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10203) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (329, 411, 1718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4496) [M::mem_pestat] mean and std.dev: (854.42, 941.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5885) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1240.554 CPU sec, 54.822 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938120, 33, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 407, 1082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2752) [M::mem_pestat] mean and std.dev: (582.94, 647.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (59, 204, 2746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8120) [M::mem_pestat] mean and std.dev: (1433.78, 1948.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10807) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (357, 452, 1094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2568) [M::mem_pestat] mean and std.dev: (723.31, 538.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3305) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1264.165 CPU sec, 72.186 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938691, 44, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (246, 343, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (363.71, 191.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.34, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (64, 689, 2754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8134) [M::mem_pestat] mean and std.dev: (1486.27, 1859.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10824) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (295, 471, 1313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3349) [M::mem_pestat] mean and std.dev: (673.45, 554.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4367) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.322 CPU sec, 54.313 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 937730, 22, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (249, 394, 531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1095) [M::mem_pestat] mean and std.dev: (421.57, 248.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.28, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (64, 254, 3135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9277) [M::mem_pestat] mean and std.dev: (2056.27, 2564.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12348) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 341, 1699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4595) [M::mem_pestat] mean and std.dev: (1091.73, 1190.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6043) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1259.564 CPU sec, 73.017 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 938456, 41, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (267, 356, 789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1833) [M::mem_pestat] mean and std.dev: (440.29, 306.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.31, 84.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (51, 340, 4457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13269) [M::mem_pestat] mean and std.dev: (2115.98, 2788.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17675) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2400000 reads in 1244.046 CPU sec, 54.772 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 938402, 28, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (272, 792, 1324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3428) [M::mem_pestat] mean and std.dev: (813.25, 607.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4480) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.25, 84.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (97, 1381, 2918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8560) [M::mem_pestat] mean and std.dev: (1759.07, 1961.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11381) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (215, 707, 1539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4187) [M::mem_pestat] mean and std.dev: (656.40, 597.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5511) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1260.956 CPU sec, 70.192 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 938533, 38, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 323, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1190) [M::mem_pestat] mean and std.dev: (343.07, 227.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.38, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 74, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1294) [M::mem_pestat] mean and std.dev: (86.59, 107.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1712) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (343, 376, 2097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5605) [M::mem_pestat] mean and std.dev: (1116.40, 1018.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7359) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1251.203 CPU sec, 68.572 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 938512, 48, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 307, 554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1216) [M::mem_pestat] mean and std.dev: (348.14, 210.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 269, 3648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10848) [M::mem_pestat] mean and std.dev: (1720.92, 2411.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14448) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (254, 313, 898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2186) [M::mem_pestat] mean and std.dev: (530.68, 460.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2830) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1234.714 CPU sec, 54.361 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 937644, 38, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 376, 909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2225) [M::mem_pestat] mean and std.dev: (479.95, 401.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.52, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 225, 2285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6755) [M::mem_pestat] mean and std.dev: (1039.40, 1525.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8990) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (250, 394, 1203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3109) [M::mem_pestat] mean and std.dev: (649.00, 724.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4062) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1265.095 CPU sec, 72.616 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 938727, 27, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (258, 962, 1289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3351) [M::mem_pestat] mean and std.dev: (705.00, 516.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.51, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 1560, 4436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13196) [M::mem_pestat] mean and std.dev: (2142.04, 2490.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17576) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (277, 913, 2450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6796) [M::mem_pestat] mean and std.dev: (1300.00, 1065.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8969) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1244.445 CPU sec, 54.786 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 938205, 33, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (344, 472, 1291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3185) [M::mem_pestat] mean and std.dev: (736.44, 612.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4132) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (210, 1528, 4154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12042) [M::mem_pestat] mean and std.dev: (2300.88, 2616.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15986) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (348, 374, 1035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2409) [M::mem_pestat] mean and std.dev: (559.36, 341.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3096) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1262.520 CPU sec, 74.327 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938832, 44, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (288, 371, 983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2373) [M::mem_pestat] mean and std.dev: (525.75, 375.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3068) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.40, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 555, 4436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13230) [M::mem_pestat] mean and std.dev: (1883.05, 2425.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17627) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (654, 1113, 3269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8499) [M::mem_pestat] mean and std.dev: (1930.81, 1821.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11114) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1245.820 CPU sec, 54.771 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 938044, 40, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 316, 1590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4354) [M::mem_pestat] mean and std.dev: (548.00, 555.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.53, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 187, 2799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8301) [M::mem_pestat] mean and std.dev: (1323.82, 1837.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11052) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (264, 327, 557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1143) [M::mem_pestat] mean and std.dev: (374.06, 227.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1436) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1261.847 CPU sec, 69.138 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938336, 40, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 343, 923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2337) [M::mem_pestat] mean and std.dev: (392.38, 313.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3044) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (81, 342, 1975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5763) [M::mem_pestat] mean and std.dev: (1033.11, 1283.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7657) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (299, 422, 1242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3128) [M::mem_pestat] mean and std.dev: (806.00, 752.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4071) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1281.492 CPU sec, 77.493 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938454, 39, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 344, 1168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2958) [M::mem_pestat] mean and std.dev: (561.04, 484.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.47, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (64, 266, 2852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8428) [M::mem_pestat] mean and std.dev: (1519.92, 2016.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11216) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (349, 875, 3103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8611) [M::mem_pestat] mean and std.dev: (1917.44, 2351.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11365) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1241.264 CPU sec, 54.668 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 938304, 34, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 342, 964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2430) [M::mem_pestat] mean and std.dev: (431.45, 295.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3163) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.40, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (49, 79, 2984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8854) [M::mem_pestat] mean and std.dev: (1453.74, 2164.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11789) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 399, 1307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3459) [M::mem_pestat] mean and std.dev: (730.05, 795.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4535) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1262.852 CPU sec, 69.950 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938382, 31, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 347, 687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1631) [M::mem_pestat] mean and std.dev: (419.06, 314.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2103) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.27, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (65, 847, 4408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13094) [M::mem_pestat] mean and std.dev: (1945.90, 2218.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17437) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 411, 878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2210) [M::mem_pestat] mean and std.dev: (542.69, 446.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2876) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1237.575 CPU sec, 54.477 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 938707, 30, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 277, 950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2368) [M::mem_pestat] mean and std.dev: (421.19, 331.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3077) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (49, 898, 2570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7612) [M::mem_pestat] mean and std.dev: (1263.75, 1538.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10133) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (389, 1164, 2221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5885) [M::mem_pestat] mean and std.dev: (1594.25, 1670.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8277) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1263.536 CPU sec, 72.337 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 937677, 37, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (243, 280, 745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1749) [M::mem_pestat] mean and std.dev: (431.35, 354.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2251) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.54, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (80, 955, 3615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10685) [M::mem_pestat] mean and std.dev: (2219.49, 2749.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14220) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (313, 894, 2012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5410) [M::mem_pestat] mean and std.dev: (1087.71, 792.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7109) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1241.276 CPU sec, 54.612 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 938900, 39, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (288, 364, 599) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1221) [M::mem_pestat] mean and std.dev: (352.00, 193.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.29, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 287, 3070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9106) [M::mem_pestat] mean and std.dev: (1954.89, 2770.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13039) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (298, 453, 840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1924) [M::mem_pestat] mean and std.dev: (484.39, 265.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2466) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1263.052 CPU sec, 75.587 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 938265, 28, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 382, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1055) [M::mem_pestat] mean and std.dev: (368.35, 225.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1335) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.48, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (81, 347, 1963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5727) [M::mem_pestat] mean and std.dev: (1015.52, 1296.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7609) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (378, 1221, 1805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4659) [M::mem_pestat] mean and std.dev: (1091.38, 841.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6086) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1258.188 CPU sec, 68.003 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938298, 32, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 291, 602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1386) [M::mem_pestat] mean and std.dev: (325.12, 300.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 164, 2898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8618) [M::mem_pestat] mean and std.dev: (1489.72, 1853.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11478) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (377, 456, 945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2081) [M::mem_pestat] mean and std.dev: (595.38, 433.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2649) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1243.074 CPU sec, 54.680 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938451, 33, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 402, 730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1562) [M::mem_pestat] mean and std.dev: (510.90, 321.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1978) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.45, 84.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (60, 570, 3280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9720) [M::mem_pestat] mean and std.dev: (1613.70, 2085.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12940) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 496, 1160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2996) [M::mem_pestat] mean and std.dev: (565.33, 365.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3914) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1254.405 CPU sec, 71.180 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938911, 41, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 281, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (270.58, 135.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.33, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 1358, 4270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12714) [M::mem_pestat] mean and std.dev: (1998.54, 2312.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16936) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (282, 1231, 2473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6855) [M::mem_pestat] mean and std.dev: (1515.86, 1207.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9046) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1234.457 CPU sec, 54.293 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 939213, 35, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 344, 1215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3179) [M::mem_pestat] mean and std.dev: (695.78, 664.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (70, 309, 2578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7594) [M::mem_pestat] mean and std.dev: (1231.64, 1705.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10102) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (325, 852, 1213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2989) [M::mem_pestat] mean and std.dev: (699.35, 455.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3877) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1259.817 CPU sec, 74.651 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 938318, 46, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (258, 727, 1477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3915) [M::mem_pestat] mean and std.dev: (822.27, 750.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5134) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.58, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (53, 249, 2645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7829) [M::mem_pestat] mean and std.dev: (1333.26, 1643.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10421) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (339, 833, 1294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3204) [M::mem_pestat] mean and std.dev: (743.83, 394.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4159) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1252.219 CPU sec, 55.058 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 938256, 46, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 320, 791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1899) [M::mem_pestat] mean and std.dev: (545.54, 541.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 320, 2216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6568) [M::mem_pestat] mean and std.dev: (1140.41, 1426.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8744) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (278, 955, 1786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4802) [M::mem_pestat] mean and std.dev: (1050.35, 828.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6310) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1262.759 CPU sec, 74.444 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 938260, 42, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 327, 803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1939) [M::mem_pestat] mean and std.dev: (547.56, 529.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (326, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (110, 650) [M::mem_pestat] mean and std.dev: (380.48, 84.07) [M::mem_pestat] low and high boundaries for proper pairs: (2, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (68, 480, 4161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12347) [M::mem_pestat] mean and std.dev: (2048.69, 2607.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16440) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (525, 899, 1540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3570) [M::mem_pestat] mean and std.dev: (909.09, 544.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4585) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1243.573 CPU sec, 54.705 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 939289, 38, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 315, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (258.18, 133.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 924) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.48, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (158, 1537, 3592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10460) [M::mem_pestat] mean and std.dev: (2301.00, 2631.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13894) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (479, 688, 1533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3641) [M::mem_pestat] mean and std.dev: (917.15, 720.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4695) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1291.488 CPU sec, 75.896 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 938503, 33, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 291, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1301) [M::mem_pestat] mean and std.dev: (378.36, 328.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 84, 2017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5961) [M::mem_pestat] mean and std.dev: (955.75, 1423.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7933) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (341, 695, 1130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2708) [M::mem_pestat] mean and std.dev: (702.06, 489.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3497) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1238.863 CPU sec, 56.549 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938430, 36, 26) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 391, 897) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2137) [M::mem_pestat] mean and std.dev: (573.21, 501.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.40, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 237, 2480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7346) [M::mem_pestat] mean and std.dev: (1291.33, 1800.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9779) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 605, 1578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4410) [M::mem_pestat] mean and std.dev: (861.32, 895.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1260.356 CPU sec, 70.300 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938247, 39, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 372, 874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2210) [M::mem_pestat] mean and std.dev: (548.96, 496.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2878) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.34, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (63, 243, 1740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5094) [M::mem_pestat] mean and std.dev: (897.51, 1350.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6771) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (356, 627, 1116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2636) [M::mem_pestat] mean and std.dev: (757.93, 543.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3396) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1243.353 CPU sec, 54.734 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 938721, 32, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 347, 1384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3688) [M::mem_pestat] mean and std.dev: (695.76, 656.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4840) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.49, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (100, 397, 2892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8476) [M::mem_pestat] mean and std.dev: (1652.19, 2039.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11268) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (218, 881, 1511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4097) [M::mem_pestat] mean and std.dev: (819.60, 685.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5390) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1268.883 CPU sec, 74.082 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 937769, 37, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (242, 362, 830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2006) [M::mem_pestat] mean and std.dev: (467.31, 410.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.35, 84.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 197, 4496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13404) [M::mem_pestat] mean and std.dev: (2150.11, 2936.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17858) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 359, 1168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3064) [M::mem_pestat] mean and std.dev: (582.94, 497.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4012) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1242.967 CPU sec, 54.876 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938475, 39, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (399, 1154, 2270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6012) [M::mem_pestat] mean and std.dev: (1276.18, 1149.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (229, 886, 2527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7123) [M::mem_pestat] mean and std.dev: (1431.44, 1440.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9421) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (322, 470, 1381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3499) [M::mem_pestat] mean and std.dev: (803.95, 799.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4558) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1245.901 CPU sec, 59.713 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938311, 27, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 430, 2106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5862) [M::mem_pestat] mean and std.dev: (1004.28, 1401.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (366, 1370, 2928) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8052) [M::mem_pestat] mean and std.dev: (1795.19, 1579.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10614) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (311, 953, 1205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2993) [M::mem_pestat] mean and std.dev: (738.42, 611.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3887) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1276.941 CPU sec, 77.118 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938365, 34, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 690, 1308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3490) [M::mem_pestat] mean and std.dev: (670.71, 531.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4581) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.26, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 124, 2826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8390) [M::mem_pestat] mean and std.dev: (1188.00, 1718.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11172) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 258, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 893) [M::mem_pestat] mean and std.dev: (210.73, 109.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1236.345 CPU sec, 54.772 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 938448, 32, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (440, 529, 1541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3743) [M::mem_pestat] mean and std.dev: (692.30, 604.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4844) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.30, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (53, 858, 2873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8513) [M::mem_pestat] mean and std.dev: (1584.03, 1994.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11333) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 485, 1476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4050) [M::mem_pestat] mean and std.dev: (627.37, 540.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5337) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1266.363 CPU sec, 74.644 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938202, 42, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 345, 586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1372) [M::mem_pestat] mean and std.dev: (388.25, 270.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.33, 84.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (62, 311, 2959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8753) [M::mem_pestat] mean and std.dev: (1922.12, 2637.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12472) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (396, 794, 1905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4923) [M::mem_pestat] mean and std.dev: (1067.80, 819.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6432) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1251.967 CPU sec, 55.151 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938213, 35, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 704, 1114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2812) [M::mem_pestat] mean and std.dev: (703.28, 584.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.26, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 231, 2927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8701) [M::mem_pestat] mean and std.dev: (1583.74, 1968.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11588) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (323, 580, 1360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3434) [M::mem_pestat] mean and std.dev: (974.13, 879.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4493) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1270.658 CPU sec, 73.677 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938798, 37, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 297, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (295.61, 166.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 271, 3443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10243) [M::mem_pestat] mean and std.dev: (1794.03, 2440.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13643) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (237, 486, 1598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4320) [M::mem_pestat] mean and std.dev: (901.00, 970.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5681) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1237.953 CPU sec, 54.495 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938200, 46, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (242, 361, 1054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2678) [M::mem_pestat] mean and std.dev: (542.39, 489.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3490) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (326, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (110, 650) [M::mem_pestat] mean and std.dev: (380.57, 84.04) [M::mem_pestat] low and high boundaries for proper pairs: (2, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 157, 1832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5418) [M::mem_pestat] mean and std.dev: (930.07, 1493.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7211) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (383, 1000, 1573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3953) [M::mem_pestat] mean and std.dev: (772.00, 448.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5143) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1260.677 CPU sec, 64.546 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938080, 26, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 548, 1097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2749) [M::mem_pestat] mean and std.dev: (696.17, 600.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3575) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (51, 191, 2210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6528) [M::mem_pestat] mean and std.dev: (1346.00, 1912.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8995) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 255, 985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2651) [M::mem_pestat] mean and std.dev: (554.62, 564.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3484) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1259.352 CPU sec, 74.590 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938470, 39, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 275, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (53, 533) [M::mem_pestat] mean and std.dev: (280.76, 52.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.50, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (55, 131, 1206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3508) [M::mem_pestat] mean and std.dev: (390.42, 722.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4659) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (269, 767, 2248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6206) [M::mem_pestat] mean and std.dev: (1113.38, 892.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8185) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1236.561 CPU sec, 54.439 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 938355, 48, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 328, 562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1198) [M::mem_pestat] mean and std.dev: (378.82, 236.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 1242, 3791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11273) [M::mem_pestat] mean and std.dev: (2394.17, 2955.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15014) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (292, 874, 1225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3091) [M::mem_pestat] mean and std.dev: (743.39, 462.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4024) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1265.861 CPU sec, 73.374 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 939030, 30, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 374, 1734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4728) [M::mem_pestat] mean and std.dev: (693.48, 707.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6225) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (209, 1492, 4435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12887) [M::mem_pestat] mean and std.dev: (2204.37, 2141.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17113) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 404, 1272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3346) [M::mem_pestat] mean and std.dev: (801.61, 890.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4383) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1238.897 CPU sec, 54.491 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 937858, 40, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 301, 823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2131) [M::mem_pestat] mean and std.dev: (540.77, 503.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2785) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.41, 84.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 161, 2700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8012) [M::mem_pestat] mean and std.dev: (1672.17, 2227.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10668) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1263.547 CPU sec, 73.682 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938905, 26, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 1131, 2421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6587) [M::mem_pestat] mean and std.dev: (1435.62, 1423.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.35, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 180, 3499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10411) [M::mem_pestat] mean and std.dev: (1406.77, 1966.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13867) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (338, 936, 1026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2402) [M::mem_pestat] mean and std.dev: (739.65, 470.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3090) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1238.262 CPU sec, 54.431 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 939108, 43, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (291, 710, 1239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3135) [M::mem_pestat] mean and std.dev: (820.82, 682.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4083) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.19, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (75, 1116, 3869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11457) [M::mem_pestat] mean and std.dev: (2079.86, 2468.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15251) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 466, 932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2318) [M::mem_pestat] mean and std.dev: (522.82, 493.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3011) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1245.606 CPU sec, 68.232 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938689, 42, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 480, 1413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3825) [M::mem_pestat] mean and std.dev: (726.00, 756.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5031) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.50, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (76, 574, 3515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10393) [M::mem_pestat] mean and std.dev: (2183.43, 2951.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13989) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 320, 1058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2756) [M::mem_pestat] mean and std.dev: (547.09, 484.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3605) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1262.203 CPU sec, 75.904 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 938282, 33, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 347, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1041) [M::mem_pestat] mean and std.dev: (366.08, 174.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1310) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.66, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 212, 2924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8668) [M::mem_pestat] mean and std.dev: (1538.84, 2221.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11540) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (260, 755, 1963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5369) [M::mem_pestat] mean and std.dev: (893.44, 803.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7072) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1248.651 CPU sec, 55.041 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 937863, 40, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (288, 793, 1701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4527) [M::mem_pestat] mean and std.dev: (930.59, 767.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5940) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (133, 1558, 2668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7738) [M::mem_pestat] mean and std.dev: (1585.14, 1609.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10273) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 195, 2577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7381) [M::mem_pestat] mean and std.dev: (812.33, 940.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9783) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1263.418 CPU sec, 72.522 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 937949, 31, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (286, 477, 2983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8377) [M::mem_pestat] mean and std.dev: (1501.69, 2032.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11074) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.33, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (70, 458, 2917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8611) [M::mem_pestat] mean and std.dev: (1454.25, 2154.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11458) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (227, 425, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1190) [M::mem_pestat] mean and std.dev: (398.17, 247.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1511) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1237.191 CPU sec, 54.453 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 938307, 29, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (254, 389, 1105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2807) [M::mem_pestat] mean and std.dev: (654.43, 584.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (75, 619, 2909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8577) [M::mem_pestat] mean and std.dev: (1380.68, 1575.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11411) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (285, 805, 1404) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3642) [M::mem_pestat] mean and std.dev: (916.00, 786.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4761) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1265.645 CPU sec, 73.567 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938975, 34, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (261, 509, 1256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3246) [M::mem_pestat] mean and std.dev: (912.71, 896.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 342, 2637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7815) [M::mem_pestat] mean and std.dev: (1455.74, 1777.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10404) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 310, 2034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5686) [M::mem_pestat] mean and std.dev: (1068.53, 1050.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7512) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1233.882 CPU sec, 54.290 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 938491, 26, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (222, 320, 1074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2778) [M::mem_pestat] mean and std.dev: (473.16, 471.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3630) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 1343, 2611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7773) [M::mem_pestat] mean and std.dev: (1292.21, 1633.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10354) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2400000 reads in 1261.828 CPU sec, 72.321 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 937603, 40, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 278, 1145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2989) [M::mem_pestat] mean and std.dev: (720.55, 844.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4100) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.48, 84.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (72, 260, 3007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8877) [M::mem_pestat] mean and std.dev: (1842.90, 2551.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12049) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (312, 629, 1127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2757) [M::mem_pestat] mean and std.dev: (803.27, 622.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3572) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1250.879 CPU sec, 66.837 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 939057, 30, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 446, 1504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3982) [M::mem_pestat] mean and std.dev: (598.24, 586.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5221) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.29, 84.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (57, 112, 2562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7572) [M::mem_pestat] mean and std.dev: (1071.41, 1489.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10077) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (397, 678, 1290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3076) [M::mem_pestat] mean and std.dev: (596.92, 320.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3969) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1233.965 CPU sec, 54.269 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938091, 32, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 342, 1266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3376) [M::mem_pestat] mean and std.dev: (564.16, 677.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.30, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (101, 1767, 3017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8849) [M::mem_pestat] mean and std.dev: (2200.50, 2515.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12262) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (320, 602, 1133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2759) [M::mem_pestat] mean and std.dev: (692.88, 512.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3572) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1259.117 CPU sec, 74.057 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 938094, 38, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 297, 1594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4356) [M::mem_pestat] mean and std.dev: (763.39, 884.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5737) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 103, 2562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7606) [M::mem_pestat] mean and std.dev: (1085.66, 1846.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10128) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (233, 473, 645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1469) [M::mem_pestat] mean and std.dev: (411.33, 239.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1881) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.692 CPU sec, 54.377 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 937768, 43, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 269, 1034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2730) [M::mem_pestat] mean and std.dev: (685.29, 743.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.27, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 861, 3297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9797) [M::mem_pestat] mean and std.dev: (2230.60, 2880.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13753) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (292, 1312, 2135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5821) [M::mem_pestat] mean and std.dev: (1625.52, 1521.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7712) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1259.393 CPU sec, 72.547 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938007, 34, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (280, 364, 1214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3082) [M::mem_pestat] mean and std.dev: (596.24, 472.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4016) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.30, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (121, 876, 4452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13114) [M::mem_pestat] mean and std.dev: (2364.29, 2959.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17445) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (290, 428, 1763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4709) [M::mem_pestat] mean and std.dev: (939.00, 1065.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6182) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1241.134 CPU sec, 54.547 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938318, 28, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (270, 361, 1146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2898) [M::mem_pestat] mean and std.dev: (522.84, 411.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3774) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.40, 84.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 116, 1122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3274) [M::mem_pestat] mean and std.dev: (495.58, 716.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4350) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 323, 1402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3778) [M::mem_pestat] mean and std.dev: (755.23, 758.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4966) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1262.028 CPU sec, 74.085 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 939036, 28, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (297, 368, 727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1587) [M::mem_pestat] mean and std.dev: (463.68, 319.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2017) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.46, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (58, 242, 4431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13177) [M::mem_pestat] mean and std.dev: (1899.71, 2575.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17550) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 292, 1308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3410) [M::mem_pestat] mean and std.dev: (575.50, 485.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4461) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1256.216 CPU sec, 69.434 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938562, 45, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 345, 1737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4731) [M::mem_pestat] mean and std.dev: (898.89, 1187.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6228) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.26, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (79, 419, 3585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10597) [M::mem_pestat] mean and std.dev: (1898.47, 2630.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14103) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (365, 861, 1502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3776) [M::mem_pestat] mean and std.dev: (842.95, 580.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4913) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1243.451 CPU sec, 54.677 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 937696, 31, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 314, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1223) [M::mem_pestat] mean and std.dev: (376.30, 251.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (69, 1120, 2949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8709) [M::mem_pestat] mean and std.dev: (1841.68, 2246.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11589) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (300, 389, 1143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2829) [M::mem_pestat] mean and std.dev: (648.55, 556.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3672) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1254.744 CPU sec, 65.325 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938506, 27, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (306, 479, 1485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3843) [M::mem_pestat] mean and std.dev: (786.94, 748.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5022) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.38, 84.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (51, 271, 2330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6888) [M::mem_pestat] mean and std.dev: (1227.78, 1578.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9167) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (320, 506, 1406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3578) [M::mem_pestat] mean and std.dev: (732.31, 702.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4664) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.270 CPU sec, 54.273 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938105, 39, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 294, 942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2504) [M::mem_pestat] mean and std.dev: (413.60, 468.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3285) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (68, 707, 2740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8084) [M::mem_pestat] mean and std.dev: (1269.03, 1545.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10756) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (363, 1055, 2126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5652) [M::mem_pestat] mean and std.dev: (1369.59, 1203.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7415) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1264.413 CPU sec, 75.138 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 938819, 43, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 324, 737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1729) [M::mem_pestat] mean and std.dev: (380.05, 328.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2225) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.61, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 167, 3554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10594) [M::mem_pestat] mean and std.dev: (1840.74, 2643.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14114) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (394, 775, 1099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2509) [M::mem_pestat] mean and std.dev: (896.72, 626.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3402) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1237.562 CPU sec, 54.465 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938553, 32, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 510, 1919) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5179) [M::mem_pestat] mean and std.dev: (896.36, 1118.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.46, 84.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 191, 4478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13362) [M::mem_pestat] mean and std.dev: (2117.50, 2793.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17804) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 314, 867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2123) [M::mem_pestat] mean and std.dev: (444.53, 316.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2751) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1259.898 CPU sec, 75.184 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 938776, 23, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 320, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1864) [M::mem_pestat] mean and std.dev: (404.50, 375.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.45, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (68, 876, 2182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6410) [M::mem_pestat] mean and std.dev: (1474.91, 1619.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8524) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (382, 848, 1566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3934) [M::mem_pestat] mean and std.dev: (1006.37, 811.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5118) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1231.465 CPU sec, 54.205 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 938321, 40, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (336, 403, 562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1014) [M::mem_pestat] mean and std.dev: (398.91, 95.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1240) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.40, 84.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (76, 1904, 3094) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9130) [M::mem_pestat] mean and std.dev: (2052.12, 2203.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12148) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (387, 1226, 1759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4503) [M::mem_pestat] mean and std.dev: (1138.27, 1002.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5875) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1276.953 CPU sec, 75.350 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 938171, 32, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 495, 1003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2535) [M::mem_pestat] mean and std.dev: (645.54, 568.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.23, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (115, 892, 4378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12904) [M::mem_pestat] mean and std.dev: (2341.47, 2795.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17167) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 348, 681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1587) [M::mem_pestat] mean and std.dev: (497.69, 427.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2207) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1231.808 CPU sec, 55.765 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938256, 45, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 604, 1111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2683) [M::mem_pestat] mean and std.dev: (647.16, 409.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 440, 2676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8002) [M::mem_pestat] mean and std.dev: (1206.98, 1504.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10665) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 294, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077) [M::mem_pestat] mean and std.dev: (291.07, 186.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1380) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1255.961 CPU sec, 72.081 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938057, 34, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 349, 949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2391) [M::mem_pestat] mean and std.dev: (421.44, 316.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3112) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (51, 1235, 3046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9036) [M::mem_pestat] mean and std.dev: (2088.45, 2732.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13018) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (301, 1117, 2138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5812) [M::mem_pestat] mean and std.dev: (1147.94, 893.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7649) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1236.459 CPU sec, 54.713 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 937978, 29, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 414, 1876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5126) [M::mem_pestat] mean and std.dev: (984.59, 1357.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6751) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.53, 84.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (85, 1000, 3583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10579) [M::mem_pestat] mean and std.dev: (2285.79, 2706.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14077) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1046, 1209, 2320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4868) [M::mem_pestat] mean and std.dev: (1194.62, 756.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6142) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1264.506 CPU sec, 74.425 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 938040, 40, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 355, 697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1705) [M::mem_pestat] mean and std.dev: (341.30, 206.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2209) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.29, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 211, 3091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9189) [M::mem_pestat] mean and std.dev: (1902.85, 2687.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12652) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 270, 414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 782) [M::mem_pestat] mean and std.dev: (265.75, 93.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1233.661 CPU sec, 54.368 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938363, 33, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (290, 500, 1857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4991) [M::mem_pestat] mean and std.dev: (802.53, 840.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6558) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.55, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 120, 4462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13318) [M::mem_pestat] mean and std.dev: (1994.24, 2654.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17746) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (371, 867, 2515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6803) [M::mem_pestat] mean and std.dev: (1201.16, 1248.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8947) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.962 CPU sec, 57.369 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 937932, 29, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 330, 756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1756) [M::mem_pestat] mean and std.dev: (409.89, 303.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2256) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.38, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 1292, 3280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9732) [M::mem_pestat] mean and std.dev: (2075.48, 2458.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12958) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (469, 1127, 3044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8194) [M::mem_pestat] mean and std.dev: (1510.67, 1417.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10769) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1288.531 CPU sec, 75.675 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 938876, 29, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 364, 913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2135) [M::mem_pestat] mean and std.dev: (495.87, 384.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.52, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 762, 4426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13166) [M::mem_pestat] mean and std.dev: (2276.72, 2933.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17536) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 915, 1688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4592) [M::mem_pestat] mean and std.dev: (978.55, 905.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6044) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1241.606 CPU sec, 56.453 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938722, 29, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (315, 363, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1164) [M::mem_pestat] mean and std.dev: (400.38, 222.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1447) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.46, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 80, 3095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9185) [M::mem_pestat] mean and std.dev: (1341.97, 2017.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12230) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (309, 969, 1533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3981) [M::mem_pestat] mean and std.dev: (830.75, 629.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5205) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1262.560 CPU sec, 75.548 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938869, 32, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 460, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1263) [M::mem_pestat] mean and std.dev: (421.14, 249.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1613) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.46, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (66, 255, 1415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4113) [M::mem_pestat] mean and std.dev: (650.76, 980.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5462) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (413, 795, 1697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4265) [M::mem_pestat] mean and std.dev: (1002.50, 740.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5549) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1241.527 CPU sec, 54.776 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 938662, 34, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (286, 370, 995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2413) [M::mem_pestat] mean and std.dev: (594.09, 452.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3122) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.31, 84.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (69, 210, 3567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10563) [M::mem_pestat] mean and std.dev: (1846.47, 2430.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14061) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 374, 862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2098) [M::mem_pestat] mean and std.dev: (484.63, 335.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2716) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1264.667 CPU sec, 71.944 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 937699, 31, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (243, 330, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1050) [M::mem_pestat] mean and std.dev: (322.12, 162.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1319) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.51, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (74, 729, 2850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8402) [M::mem_pestat] mean and std.dev: (1559.77, 1768.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11178) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (287, 677, 921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2189) [M::mem_pestat] mean and std.dev: (678.75, 472.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2823) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1237.784 CPU sec, 54.545 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 938177, 39, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 365, 1042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2630) [M::mem_pestat] mean and std.dev: (431.11, 336.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3424) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.48, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (76, 963, 3727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11029) [M::mem_pestat] mean and std.dev: (2153.08, 2625.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14680) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 361, 1239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3245) [M::mem_pestat] mean and std.dev: (601.86, 560.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4248) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1249.159 CPU sec, 62.115 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938651, 49, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (476, 995, 2703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7157) [M::mem_pestat] mean and std.dev: (1887.32, 1806.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.40, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 1940, 3311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9837) [M::mem_pestat] mean and std.dev: (2120.85, 2422.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13100) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 788, 2111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5875) [M::mem_pestat] mean and std.dev: (1302.31, 1477.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7757) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1264.485 CPU sec, 73.672 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 937733, 36, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (258, 308, 1199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3081) [M::mem_pestat] mean and std.dev: (658.25, 608.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4022) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (101, 972, 3600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10598) [M::mem_pestat] mean and std.dev: (1834.69, 2262.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14097) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (583, 921, 1609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3661) [M::mem_pestat] mean and std.dev: (983.50, 552.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4687) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1238.703 CPU sec, 54.554 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 938657, 31, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (158, 426, 1292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3560) [M::mem_pestat] mean and std.dev: (691.29, 753.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.46, 84.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (61, 100, 2671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7891) [M::mem_pestat] mean and std.dev: (1289.20, 2030.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10501) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (303, 400, 1176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2922) [M::mem_pestat] mean and std.dev: (766.14, 701.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3795) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1256.440 CPU sec, 72.488 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 938984, 29, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (300, 317, 1970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5310) [M::mem_pestat] mean and std.dev: (795.54, 825.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.53, 84.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 344, 4475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13325) [M::mem_pestat] mean and std.dev: (2353.03, 3110.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17750) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 350, 744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1808) [M::mem_pestat] mean and std.dev: (461.69, 365.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2340) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1233.451 CPU sec, 54.261 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938542, 46, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 284, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023) [M::mem_pestat] mean and std.dev: (308.91, 141.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1296) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.49, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 213, 2671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7967) [M::mem_pestat] mean and std.dev: (1517.85, 2054.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10615) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (293, 768, 1129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2801) [M::mem_pestat] mean and std.dev: (690.62, 476.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3637) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1258.676 CPU sec, 72.532 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 938203, 35, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 468, 645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1537) [M::mem_pestat] mean and std.dev: (364.57, 219.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.48, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 198, 2227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6605) [M::mem_pestat] mean and std.dev: (1214.88, 1671.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8794) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2400000 reads in 1238.820 CPU sec, 54.446 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938719, 32, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (285, 315, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993) [M::mem_pestat] mean and std.dev: (386.90, 202.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1229) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (58, 371, 1868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5488) [M::mem_pestat] mean and std.dev: (1022.28, 1298.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7298) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (382, 552, 993) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2215) [M::mem_pestat] mean and std.dev: (518.40, 308.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1245.587 CPU sec, 65.303 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 937603, 40, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 361, 1277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3415) [M::mem_pestat] mean and std.dev: (776.24, 750.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4484) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (94, 1079, 2959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8689) [M::mem_pestat] mean and std.dev: (1942.72, 2340.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11554) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (249, 941, 1937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5313) [M::mem_pestat] mean and std.dev: (870.20, 732.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7001) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1260.266 CPU sec, 72.375 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 937813, 34, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 306, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1303) [M::mem_pestat] mean and std.dev: (412.14, 308.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.57, 84.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (152, 1337, 4475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13121) [M::mem_pestat] mean and std.dev: (2516.24, 2983.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17444) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (216, 792, 1045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2703) [M::mem_pestat] mean and std.dev: (635.82, 550.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3532) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1239.543 CPU sec, 54.695 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 938371, 38, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 328, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1243) [M::mem_pestat] mean and std.dev: (345.92, 189.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 1954, 3075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9137) [M::mem_pestat] mean and std.dev: (2022.53, 2250.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12168) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 809, 1357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3561) [M::mem_pestat] mean and std.dev: (757.33, 576.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4663) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1257.768 CPU sec, 72.984 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938538, 28, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (279, 392, 703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1551) [M::mem_pestat] mean and std.dev: (426.00, 250.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1975) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (89, 1667, 4428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13106) [M::mem_pestat] mean and std.dev: (2120.93, 2082.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17445) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (337, 872, 1365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3421) [M::mem_pestat] mean and std.dev: (880.94, 672.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4449) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1238.559 CPU sec, 54.454 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 938000, 35, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 359, 879) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2187) [M::mem_pestat] mean and std.dev: (436.12, 336.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2841) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.48, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 70, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1476) [M::mem_pestat] mean and std.dev: (146.13, 212.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1957) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (256, 1050, 3141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8911) [M::mem_pestat] mean and std.dev: (1913.93, 2301.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11796) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1260.033 CPU sec, 72.034 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 938494, 32, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 316, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (293.14, 93.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1076) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.38, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (59, 328, 2950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8732) [M::mem_pestat] mean and std.dev: (1600.75, 2170.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11623) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (289, 721, 2046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5560) [M::mem_pestat] mean and std.dev: (879.86, 750.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.892 CPU sec, 54.366 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 938611, 30, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 378, 622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1340) [M::mem_pestat] mean and std.dev: (495.44, 332.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.24, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (53, 216, 2564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7586) [M::mem_pestat] mean and std.dev: (1258.62, 1737.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10097) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (290, 765, 2710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7550) [M::mem_pestat] mean and std.dev: (1471.95, 1884.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9970) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1250.581 CPU sec, 67.505 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938387, 31, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 396, 1025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2743) [M::mem_pestat] mean and std.dev: (486.26, 603.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 119, 2322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6870) [M::mem_pestat] mean and std.dev: (835.29, 1393.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9144) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (280, 313, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2911) [M::mem_pestat] mean and std.dev: (664.00, 532.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3788) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1258.195 CPU sec, 75.938 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (35, 939257, 26, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 347, 1010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2506) [M::mem_pestat] mean and std.dev: (481.73, 393.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3254) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.31, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 112, 2618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7802) [M::mem_pestat] mean and std.dev: (1147.52, 1762.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10394) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (319, 1146, 1691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4435) [M::mem_pestat] mean and std.dev: (1111.00, 1076.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5807) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1233.606 CPU sec, 54.357 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 939219, 32, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 359, 752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1760) [M::mem_pestat] mean and std.dev: (443.48, 392.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2264) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.34, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 170, 3063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9105) [M::mem_pestat] mean and std.dev: (1852.56, 2818.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13126) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (674, 1094, 4567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12353) [M::mem_pestat] mean and std.dev: (2221.71, 2408.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16246) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1254.758 CPU sec, 73.481 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 938238, 33, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (371, 527, 817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1709) [M::mem_pestat] mean and std.dev: (502.05, 260.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2155) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.49, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 95, 2857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8499) [M::mem_pestat] mean and std.dev: (1182.94, 1665.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11320) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (344, 440, 1477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3743) [M::mem_pestat] mean and std.dev: (728.18, 759.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4876) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1237.897 CPU sec, 54.541 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 938713, 29, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 391, 922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2238) [M::mem_pestat] mean and std.dev: (543.43, 487.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.38, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (64, 233, 2589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7639) [M::mem_pestat] mean and std.dev: (1139.31, 1511.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10164) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (340, 690, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2326) [M::mem_pestat] mean and std.dev: (617.56, 344.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2988) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1252.889 CPU sec, 71.099 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 938832, 25, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 451, 1723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4665) [M::mem_pestat] mean and std.dev: (735.17, 669.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6136) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (72, 1451, 4455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13221) [M::mem_pestat] mean and std.dev: (2103.84, 2236.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17604) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (368, 945, 1134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2666) [M::mem_pestat] mean and std.dev: (779.87, 442.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3432) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1239.348 CPU sec, 54.525 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938051, 38, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (309, 425, 1209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3009) [M::mem_pestat] mean and std.dev: (636.56, 465.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3909) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 87, 2680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7952) [M::mem_pestat] mean and std.dev: (1019.86, 1606.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10588) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (343, 879, 1456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3682) [M::mem_pestat] mean and std.dev: (799.50, 663.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4795) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1265.100 CPU sec, 73.987 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 938161, 34, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (243, 317, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (271.64, 90.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 979) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.49, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (122, 1454, 3977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11687) [M::mem_pestat] mean and std.dev: (2370.24, 2585.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15542) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 425, 1433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3815) [M::mem_pestat] mean and std.dev: (758.92, 662.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5006) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1240.405 CPU sec, 63.412 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938150, 41, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (270, 354, 759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1737) [M::mem_pestat] mean and std.dev: (422.06, 270.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2226) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.41, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 529, 1571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4605) [M::mem_pestat] mean and std.dev: (858.54, 1110.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6122) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 907, 2574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7316) [M::mem_pestat] mean and std.dev: (1182.42, 1240.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9687) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1229.269 CPU sec, 54.018 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 939182, 28, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 303, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (259.72, 111.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.20, 84.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 1353, 4187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12497) [M::mem_pestat] mean and std.dev: (1870.36, 1979.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16652) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (346, 966, 2107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5629) [M::mem_pestat] mean and std.dev: (899.77, 743.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7390) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1255.603 CPU sec, 72.692 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 937452, 42, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 333, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2574) [M::mem_pestat] mean and std.dev: (527.21, 510.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.65, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (75, 487, 4463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13239) [M::mem_pestat] mean and std.dev: (2064.81, 2770.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17627) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (378, 1408, 2172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5760) [M::mem_pestat] mean and std.dev: (1495.44, 1322.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7554) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1242.287 CPU sec, 54.652 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 938406, 38, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 755, 1057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2629) [M::mem_pestat] mean and std.dev: (648.62, 456.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3415) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 112, 2667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7935) [M::mem_pestat] mean and std.dev: (1025.77, 1870.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10569) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (225, 317, 1063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2739) [M::mem_pestat] mean and std.dev: (511.21, 462.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3577) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1258.896 CPU sec, 74.089 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 937918, 40, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 310, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2122) [M::mem_pestat] mean and std.dev: (473.95, 445.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2764) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (164, 1767, 3055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8837) [M::mem_pestat] mean and std.dev: (2031.33, 2051.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11728) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 336, 1220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3240) [M::mem_pestat] mean and std.dev: (638.91, 511.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4250) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1238.578 CPU sec, 54.596 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938019, 36, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 295, 1194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3176) [M::mem_pestat] mean and std.dev: (536.94, 523.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4167) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.48, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (82, 800, 2855) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8401) [M::mem_pestat] mean and std.dev: (1713.63, 2015.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11174) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (383, 678, 1655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4199) [M::mem_pestat] mean and std.dev: (975.41, 963.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5471) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1263.332 CPU sec, 75.152 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 938663, 34, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 306, 893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2189) [M::mem_pestat] mean and std.dev: (523.72, 502.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.47, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (60, 1447, 2608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7704) [M::mem_pestat] mean and std.dev: (1751.24, 2085.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10252) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2400000 reads in 1250.762 CPU sec, 66.986 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938753, 40, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 286, 345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (232.07, 82.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.51, 84.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 577, 2637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7819) [M::mem_pestat] mean and std.dev: (1350.16, 1588.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10410) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (326, 842, 1306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3266) [M::mem_pestat] mean and std.dev: (828.15, 692.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4246) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1236.504 CPU sec, 54.354 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 938894, 34, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 310, 812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2114) [M::mem_pestat] mean and std.dev: (395.92, 383.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (64, 316, 3047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9013) [M::mem_pestat] mean and std.dev: (1914.97, 2571.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12202) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1256.289 CPU sec, 71.647 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 938094, 35, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 309, 1359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3627) [M::mem_pestat] mean and std.dev: (494.81, 473.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4761) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.41, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (75, 202, 2554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7512) [M::mem_pestat] mean and std.dev: (1300.49, 1708.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9991) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (303, 468, 1516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3942) [M::mem_pestat] mean and std.dev: (693.33, 616.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5155) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1237.455 CPU sec, 54.393 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938725, 41, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 285, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (261.95, 89.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.53, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 220, 3044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9058) [M::mem_pestat] mean and std.dev: (1613.51, 2163.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12065) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (688, 798, 1126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2002) [M::mem_pestat] mean and std.dev: (712.55, 268.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2440) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1252.276 CPU sec, 73.301 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 939267, 38, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 262, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (246.35, 120.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.33, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (63, 592, 3270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9684) [M::mem_pestat] mean and std.dev: (1664.21, 1865.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12891) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (305, 554, 2052) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5546) [M::mem_pestat] mean and std.dev: (1057.26, 1093.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7293) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1230.131 CPU sec, 54.060 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 937970, 33, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 318, 795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1913) [M::mem_pestat] mean and std.dev: (519.86, 485.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2472) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.22, 84.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 143, 1936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5748) [M::mem_pestat] mean and std.dev: (1157.85, 1641.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7724) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (510, 1131, 2019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5037) [M::mem_pestat] mean and std.dev: (1217.59, 1013.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6546) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1257.908 CPU sec, 73.927 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 939102, 37, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (182, 310, 963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2525) [M::mem_pestat] mean and std.dev: (457.00, 484.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3306) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 353, 2678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7956) [M::mem_pestat] mean and std.dev: (1600.46, 1911.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10595) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (792, 914, 1329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2403) [M::mem_pestat] mean and std.dev: (906.67, 483.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2940) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.798 CPU sec, 54.353 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 938171, 40, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 381, 2520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7182) [M::mem_pestat] mean and std.dev: (1211.33, 1804.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.35, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (63, 770, 3549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10521) [M::mem_pestat] mean and std.dev: (2037.72, 2589.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14007) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1292, 1544, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5231) [M::mem_pestat] mean and std.dev: (1772.31, 990.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6544) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1274.038 CPU sec, 74.946 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938491, 39, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 307, 1579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4241) [M::mem_pestat] mean and std.dev: (612.52, 793.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5572) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.47, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 127, 1906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5636) [M::mem_pestat] mean and std.dev: (1010.39, 1500.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7501) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (222, 359, 1204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3168) [M::mem_pestat] mean and std.dev: (478.75, 400.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4150) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1228.312 CPU sec, 55.731 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 937876, 35, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 418, 1022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2520) [M::mem_pestat] mean and std.dev: (602.67, 568.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3269) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.41, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (269, 1367, 4402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12668) [M::mem_pestat] mean and std.dev: (2213.34, 2426.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16801) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (304, 844, 2080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5632) [M::mem_pestat] mean and std.dev: (1292.42, 1422.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7408) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1255.893 CPU sec, 72.442 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 938157, 40, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 462, 1607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4431) [M::mem_pestat] mean and std.dev: (779.94, 795.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5843) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.40, 84.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (101, 971, 4029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11885) [M::mem_pestat] mean and std.dev: (2088.80, 2503.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15813) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 1187, 2075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5755) [M::mem_pestat] mean and std.dev: (1026.25, 851.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7595) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1230.408 CPU sec, 54.506 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938132, 30, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (304, 646, 1435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3697) [M::mem_pestat] mean and std.dev: (715.31, 605.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4828) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.47, 84.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 436, 2178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6440) [M::mem_pestat] mean and std.dev: (1154.79, 1453.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8571) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (356, 1047, 2579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7025) [M::mem_pestat] mean and std.dev: (1619.76, 1625.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9248) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1249.550 CPU sec, 71.324 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 937844, 45, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 357, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 959) [M::mem_pestat] mean and std.dev: (321.33, 151.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (62, 376, 4449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13223) [M::mem_pestat] mean and std.dev: (2128.51, 2641.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17610) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (750, 1245, 2774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6822) [M::mem_pestat] mean and std.dev: (1926.25, 2116.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10392) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1232.270 CPU sec, 54.343 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 937487, 41, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 348, 592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1328) [M::mem_pestat] mean and std.dev: (459.89, 346.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1847) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (156, 2150, 4439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13005) [M::mem_pestat] mean and std.dev: (2798.83, 2947.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17288) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (313, 457, 2864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7966) [M::mem_pestat] mean and std.dev: (1592.73, 2012.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10517) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1237.659 CPU sec, 58.422 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938248, 38, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (129, 348, 995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2727) [M::mem_pestat] mean and std.dev: (391.82, 483.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.51, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (79, 430, 3111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9175) [M::mem_pestat] mean and std.dev: (1750.58, 2337.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12207) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1263.155 CPU sec, 74.998 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 937933, 30, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (293, 340, 749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1661) [M::mem_pestat] mean and std.dev: (464.00, 305.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2117) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.47, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (59, 260, 1911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5615) [M::mem_pestat] mean and std.dev: (1203.60, 1549.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7467) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (344, 556, 1006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2330) [M::mem_pestat] mean and std.dev: (625.08, 344.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2992) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1230.193 CPU sec, 54.093 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 939227, 44, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 387, 980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2450) [M::mem_pestat] mean and std.dev: (473.33, 410.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3185) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.33, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 136, 2575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7681) [M::mem_pestat] mean and std.dev: (1191.35, 1754.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10234) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (193, 328, 1053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2773) [M::mem_pestat] mean and std.dev: (690.69, 729.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3633) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1248.831 CPU sec, 73.022 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 938673, 31, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 350, 914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2194) [M::mem_pestat] mean and std.dev: (458.09, 378.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (66, 806, 4434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13170) [M::mem_pestat] mean and std.dev: (2028.13, 2460.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17538) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (290, 481, 1278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3254) [M::mem_pestat] mean and std.dev: (652.94, 518.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4242) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1233.507 CPU sec, 54.309 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 937801, 36, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (249, 322, 738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1716) [M::mem_pestat] mean and std.dev: (428.44, 352.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2205) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (49, 253, 2583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7651) [M::mem_pestat] mean and std.dev: (1357.28, 1814.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10185) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (304, 1334, 2730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7582) [M::mem_pestat] mean and std.dev: (1255.30, 974.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10008) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1251.350 CPU sec, 68.008 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 937962, 45, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 335, 1151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3075) [M::mem_pestat] mean and std.dev: (635.10, 708.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4037) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 71, 1429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4211) [M::mem_pestat] mean and std.dev: (800.14, 1182.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5602) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (480, 1119, 1858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4614) [M::mem_pestat] mean and std.dev: (936.36, 634.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5992) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1237.414 CPU sec, 54.372 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 938172, 33, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (287, 326, 1526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4004) [M::mem_pestat] mean and std.dev: (875.55, 1102.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5286) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.58, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 146, 2592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7708) [M::mem_pestat] mean and std.dev: (1285.62, 1934.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10266) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (332, 1039, 1568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4040) [M::mem_pestat] mean and std.dev: (872.65, 600.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5276) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1240.100 CPU sec, 63.174 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 939319, 34, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 299, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1117) [M::mem_pestat] mean and std.dev: (313.23, 196.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.35, 84.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (51, 1532, 4462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13284) [M::mem_pestat] mean and std.dev: (2410.06, 2855.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17695) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 471, 1241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3355) [M::mem_pestat] mean and std.dev: (657.89, 568.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4412) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1262.605 CPU sec, 77.472 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938338, 29, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (259, 435, 1243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3211) [M::mem_pestat] mean and std.dev: (606.05, 525.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4195) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.32, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (105, 1746, 4441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13113) [M::mem_pestat] mean and std.dev: (2498.24, 2578.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17449) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (242, 675, 1030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2606) [M::mem_pestat] mean and std.dev: (749.77, 650.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3394) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1229.262 CPU sec, 54.217 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 938363, 27, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (500, 894, 3455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9365) [M::mem_pestat] mean and std.dev: (2140.74, 2548.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12336) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.54, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (57, 185, 2652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7842) [M::mem_pestat] mean and std.dev: (1164.23, 1465.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10437) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (188, 408, 1212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3260) [M::mem_pestat] mean and std.dev: (554.83, 495.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4284) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1259.695 CPU sec, 73.275 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 937818, 30, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 457, 1129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2833) [M::mem_pestat] mean and std.dev: (553.56, 525.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 126, 1483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4389) [M::mem_pestat] mean and std.dev: (749.70, 1057.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5842) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (343, 1097, 3998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11308) [M::mem_pestat] mean and std.dev: (2146.53, 2262.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14963) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1236.894 CPU sec, 54.564 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 937651, 49, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (261, 380, 1104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2790) [M::mem_pestat] mean and std.dev: (671.93, 643.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3633) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 248, 2642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7830) [M::mem_pestat] mean and std.dev: (1073.30, 1562.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10424) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2400000 reads in 1255.173 CPU sec, 67.363 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 938762, 43, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 277, 525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1189) [M::mem_pestat] mean and std.dev: (367.54, 249.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.56, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (77, 464, 2879) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8483) [M::mem_pestat] mean and std.dev: (1663.05, 2179.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11285) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (289, 463, 2673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7441) [M::mem_pestat] mean and std.dev: (1564.59, 1891.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9825) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1228.736 CPU sec, 53.976 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 937824, 34, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 382, 1264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3226) [M::mem_pestat] mean and std.dev: (659.21, 631.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4207) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.56, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 156, 1871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5537) [M::mem_pestat] mean and std.dev: (990.88, 1398.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7370) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (308, 730, 2088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5648) [M::mem_pestat] mean and std.dev: (1272.15, 1371.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7428) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1254.618 CPU sec, 68.401 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (29, 938855, 29, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 290, 807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2053) [M::mem_pestat] mean and std.dev: (422.59, 429.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2676) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.32, 84.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 694, 3649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10843) [M::mem_pestat] mean and std.dev: (2020.38, 2492.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14440) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (262, 668, 976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2404) [M::mem_pestat] mean and std.dev: (633.53, 497.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3118) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1249.253 CPU sec, 69.202 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 938581, 38, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 315, 917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2333) [M::mem_pestat] mean and std.dev: (478.82, 433.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3041) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (73, 898, 2932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8650) [M::mem_pestat] mean and std.dev: (1627.74, 1796.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11509) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (329, 871, 1504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3854) [M::mem_pestat] mean and std.dev: (790.21, 715.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5029) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1230.004 CPU sec, 54.114 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938977, 41, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 361, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1011) [M::mem_pestat] mean and std.dev: (319.79, 91.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1257) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 1264, 4419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13191) [M::mem_pestat] mean and std.dev: (2198.90, 2729.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17577) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (288, 1073, 1471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3837) [M::mem_pestat] mean and std.dev: (815.56, 624.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5020) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1253.923 CPU sec, 71.498 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938887, 27, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 379, 1834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4956) [M::mem_pestat] mean and std.dev: (993.81, 1128.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.40, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 733, 2054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6072) [M::mem_pestat] mean and std.dev: (959.33, 1367.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8081) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (232, 390, 746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1774) [M::mem_pestat] mean and std.dev: (409.80, 224.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2288) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1232.809 CPU sec, 54.269 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938042, 26, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 399, 500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 954) [M::mem_pestat] mean and std.dev: (348.75, 125.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 227, 2723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8093) [M::mem_pestat] mean and std.dev: (1347.44, 1538.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10778) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (231, 931, 2082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5784) [M::mem_pestat] mean and std.dev: (1228.67, 1190.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7635) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1260.582 CPU sec, 72.235 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 938622, 21, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (246, 337, 561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1191) [M::mem_pestat] mean and std.dev: (323.82, 167.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.24, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 230, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7708) [M::mem_pestat] mean and std.dev: (1094.86, 1305.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10262) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (696, 1276, 1952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4464) [M::mem_pestat] mean and std.dev: (1302.81, 984.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5720) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1234.524 CPU sec, 54.313 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 938674, 37, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 887, 2020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5410) [M::mem_pestat] mean and std.dev: (966.20, 921.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7105) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.50, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 407, 2623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7811) [M::mem_pestat] mean and std.dev: (1376.25, 1813.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10405) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2400000 reads in 1256.219 CPU sec, 72.207 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938540, 35, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 294, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (295.84, 169.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.47, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (96, 736, 4478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13242) [M::mem_pestat] mean and std.dev: (2513.74, 3105.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17624) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1252.763 CPU sec, 69.141 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938466, 40, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 292, 888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2232) [M::mem_pestat] mean and std.dev: (502.57, 531.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2904) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 199, 2905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8623) [M::mem_pestat] mean and std.dev: (1534.36, 2303.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11482) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (191, 357, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1916) [M::mem_pestat] mean and std.dev: (471.90, 376.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2491) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1228.540 CPU sec, 54.102 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 939037, 34, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 407, 856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2058) [M::mem_pestat] mean and std.dev: (507.47, 449.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.41, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (61, 976, 2977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8809) [M::mem_pestat] mean and std.dev: (1862.88, 2334.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11725) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (189, 376, 1640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4542) [M::mem_pestat] mean and std.dev: (422.90, 432.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5993) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1253.892 CPU sec, 69.462 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 939224, 37, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (336, 362, 400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (208, 528) [M::mem_pestat] mean and std.dev: (351.29, 28.52) [M::mem_pestat] low and high boundaries for proper pairs: (144, 592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (326, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (110, 650) [M::mem_pestat] mean and std.dev: (380.51, 84.05) [M::mem_pestat] low and high boundaries for proper pairs: (2, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 140, 2616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7788) [M::mem_pestat] mean and std.dev: (1084.56, 1720.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10374) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (324, 1293, 3124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8724) [M::mem_pestat] mean and std.dev: (1975.83, 2059.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11524) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1230.646 CPU sec, 54.133 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 938993, 29, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (269, 349, 1339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3479) [M::mem_pestat] mean and std.dev: (622.83, 617.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 115, 2631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7825) [M::mem_pestat] mean and std.dev: (1297.41, 1834.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10422) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 466, 1148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2942) [M::mem_pestat] mean and std.dev: (610.60, 470.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3839) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1250.657 CPU sec, 71.988 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 939201, 42, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 342, 727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1715) [M::mem_pestat] mean and std.dev: (451.56, 343.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2209) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (94, 942, 2067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6013) [M::mem_pestat] mean and std.dev: (1382.97, 1578.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7986) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (261, 781, 3086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8736) [M::mem_pestat] mean and std.dev: (1706.06, 1983.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11561) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1230.355 CPU sec, 54.045 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 937600, 38, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 271, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1022) [M::mem_pestat] mean and std.dev: (280.80, 181.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.25, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (81, 451, 2998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8832) [M::mem_pestat] mean and std.dev: (1750.84, 2271.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11749) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (192, 486, 1220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3276) [M::mem_pestat] mean and std.dev: (581.60, 527.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4304) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1254.996 CPU sec, 72.982 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938949, 29, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (337, 760, 1150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2776) [M::mem_pestat] mean and std.dev: (668.83, 502.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3589) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.34, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (51, 1895, 4469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13305) [M::mem_pestat] mean and std.dev: (2232.21, 2397.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17723) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 685, 1641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4413) [M::mem_pestat] mean and std.dev: (943.00, 914.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5799) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1249.263 CPU sec, 68.168 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (33, 938845, 24, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 761, 1952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5258) [M::mem_pestat] mean and std.dev: (891.55, 864.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6911) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (75, 219, 2613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7689) [M::mem_pestat] mean and std.dev: (1135.87, 1417.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10227) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (277, 390, 826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1924) [M::mem_pestat] mean and std.dev: (481.69, 398.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2473) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1231.503 CPU sec, 54.147 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938687, 28, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 301, 580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1286) [M::mem_pestat] mean and std.dev: (336.89, 232.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.45, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (220, 2585, 3736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10768) [M::mem_pestat] mean and std.dev: (2315.00, 2123.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14284) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (372, 961, 2319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6213) [M::mem_pestat] mean and std.dev: (1507.44, 1660.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8160) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1258.702 CPU sec, 73.313 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938713, 34, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 316, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1128) [M::mem_pestat] mean and std.dev: (351.95, 273.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1448) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.38, 84.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (70, 1847, 2942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8686) [M::mem_pestat] mean and std.dev: (2017.24, 2411.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11662) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (491, 851, 2286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5876) [M::mem_pestat] mean and std.dev: (1179.62, 808.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7671) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1234.485 CPU sec, 54.347 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 939284, 38, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 532, 1683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4401) [M::mem_pestat] mean and std.dev: (885.27, 915.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (181, 1604, 3482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10084) [M::mem_pestat] mean and std.dev: (2108.16, 2283.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13385) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (198, 332, 758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1878) [M::mem_pestat] mean and std.dev: (520.88, 490.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2484) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1251.506 CPU sec, 73.411 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938816, 31, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 532, 1038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2668) [M::mem_pestat] mean and std.dev: (537.39, 570.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.34, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (113, 1566, 3061) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8957) [M::mem_pestat] mean and std.dev: (1979.00, 2068.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11905) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (244, 332, 1294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3394) [M::mem_pestat] mean and std.dev: (717.44, 767.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4444) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1228.854 CPU sec, 54.065 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 938957, 31, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (309, 372, 1179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2919) [M::mem_pestat] mean and std.dev: (556.60, 463.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3789) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.55, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 122, 2201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6491) [M::mem_pestat] mean and std.dev: (880.20, 1401.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8636) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (852, 1082, 1646) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3234) [M::mem_pestat] mean and std.dev: (1177.12, 599.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4028) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1255.603 CPU sec, 72.981 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (31, 938846, 28, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 350, 1500) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3974) [M::mem_pestat] mean and std.dev: (739.50, 842.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5211) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (62, 184, 3409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10103) [M::mem_pestat] mean and std.dev: (1284.89, 1791.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13450) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (756, 1272, 3822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9954) [M::mem_pestat] mean and std.dev: (2158.08, 2374.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13020) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1234.771 CPU sec, 54.345 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (41, 938134, 45, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 453, 1305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3511) [M::mem_pestat] mean and std.dev: (690.70, 680.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.49, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (72, 896, 3136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9264) [M::mem_pestat] mean and std.dev: (1946.09, 2371.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12328) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (419, 1066, 1472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3578) [M::mem_pestat] mean and std.dev: (1087.36, 774.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4631) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1271.275 CPU sec, 75.223 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938856, 29, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 310, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1094) [M::mem_pestat] mean and std.dev: (289.75, 167.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1397) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.47, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 228, 4438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13270) [M::mem_pestat] mean and std.dev: (1926.17, 2439.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17686) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (351, 756, 1101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2601) [M::mem_pestat] mean and std.dev: (757.70, 645.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3351) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1232.682 CPU sec, 56.094 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 939366, 39, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 452, 1641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4497) [M::mem_pestat] mean and std.dev: (762.83, 728.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5925) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.55, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (53, 170, 3662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10880) [M::mem_pestat] mean and std.dev: (2139.46, 3023.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14489) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1249.017 CPU sec, 68.214 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 938633, 27, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 310, 657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1541) [M::mem_pestat] mean and std.dev: (371.67, 264.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.38, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 244, 2853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8511) [M::mem_pestat] mean and std.dev: (1462.52, 1704.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11340) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (322, 668, 1165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2851) [M::mem_pestat] mean and std.dev: (712.24, 456.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3694) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1230.706 CPU sec, 54.500 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 938505, 33, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (490, 992, 1594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3802) [M::mem_pestat] mean and std.dev: (831.15, 504.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4906) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.53, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 1019, 2610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7760) [M::mem_pestat] mean and std.dev: (1450.13, 1763.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10335) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (821, 1169, 1512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2894) [M::mem_pestat] mean and std.dev: (1080.00, 545.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3585) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1258.701 CPU sec, 72.485 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938259, 26, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 383, 1697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4563) [M::mem_pestat] mean and std.dev: (566.00, 689.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5996) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (99, 1462, 4465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13197) [M::mem_pestat] mean and std.dev: (2260.23, 2638.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17563) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (325, 852, 1323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3319) [M::mem_pestat] mean and std.dev: (788.64, 528.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1240.197 CPU sec, 54.575 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 938158, 50, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 339, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2149) [M::mem_pestat] mean and std.dev: (523.65, 442.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.31, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (55, 209, 2632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7786) [M::mem_pestat] mean and std.dev: (1165.52, 1592.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10363) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (287, 476, 989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2393) [M::mem_pestat] mean and std.dev: (669.85, 551.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1228.093 CPU sec, 55.884 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 938240, 40, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (293, 441, 1058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2588) [M::mem_pestat] mean and std.dev: (617.27, 493.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3353) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.45, 84.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (103, 2206, 3634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10696) [M::mem_pestat] mean and std.dev: (2445.25, 2687.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14227) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 509, 823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1937) [M::mem_pestat] mean and std.dev: (536.56, 290.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2494) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1276.106 CPU sec, 75.175 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 938130, 40, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (186, 294, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 927) [M::mem_pestat] mean and std.dev: (248.21, 101.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.25, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 118, 1817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5391) [M::mem_pestat] mean and std.dev: (839.31, 1345.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7178) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2400000 reads in 1237.668 CPU sec, 56.538 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 937650, 43, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 383, 1342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3526) [M::mem_pestat] mean and std.dev: (731.82, 645.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.56, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 100, 4443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13289) [M::mem_pestat] mean and std.dev: (1974.23, 2628.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17712) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 738, 2773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7841) [M::mem_pestat] mean and std.dev: (1361.93, 1527.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10375) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1254.893 CPU sec, 72.128 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 938505, 36, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 356, 609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1345) [M::mem_pestat] mean and std.dev: (389.36, 320.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.45, 84.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 650, 3133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9325) [M::mem_pestat] mean and std.dev: (1858.36, 2279.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12421) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2400000 reads in 1243.360 CPU sec, 54.899 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938147, 30, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (276, 310, 997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2439) [M::mem_pestat] mean and std.dev: (468.65, 394.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3160) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.52, 84.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 118, 663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1923) [M::mem_pestat] mean and std.dev: (177.12, 317.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2553) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (324, 800, 1812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4788) [M::mem_pestat] mean and std.dev: (1054.60, 1094.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6276) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1261.648 CPU sec, 73.789 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 938339, 33, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (272, 440, 954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2318) [M::mem_pestat] mean and std.dev: (663.89, 535.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3000) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (84, 623, 4426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13110) [M::mem_pestat] mean and std.dev: (2224.30, 2760.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17452) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (342, 747, 1377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3447) [M::mem_pestat] mean and std.dev: (849.44, 617.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4482) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1237.772 CPU sec, 54.617 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 938659, 29, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 343, 931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2247) [M::mem_pestat] mean and std.dev: (542.12, 396.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2905) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.45, 84.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (133, 1193, 4522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13300) [M::mem_pestat] mean and std.dev: (2376.86, 2626.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17689) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (451, 584, 1075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2323) [M::mem_pestat] mean and std.dev: (617.53, 313.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2947) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1230.573 CPU sec, 60.282 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938006, 36, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (209, 404, 2195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6167) [M::mem_pestat] mean and std.dev: (1270.62, 1675.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8153) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.38, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 80, 2695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8011) [M::mem_pestat] mean and std.dev: (1399.42, 2119.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10669) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (335, 671, 983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2279) [M::mem_pestat] mean and std.dev: (590.60, 414.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2927) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1273.462 CPU sec, 76.300 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 937805, 33, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (239, 342, 764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1814) [M::mem_pestat] mean and std.dev: (468.32, 406.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.34, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 85, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7764) [M::mem_pestat] mean and std.dev: (1093.88, 1487.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10343) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 394, 1154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2984) [M::mem_pestat] mean and std.dev: (630.06, 594.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3899) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1236.392 CPU sec, 54.694 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 938437, 32, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 367, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (307.00, 97.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (68, 220, 4510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13394) [M::mem_pestat] mean and std.dev: (2269.94, 3018.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17836) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (319, 809, 1488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3826) [M::mem_pestat] mean and std.dev: (916.67, 720.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4995) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1251.153 CPU sec, 71.965 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938004, 36, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 301, 1121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3021) [M::mem_pestat] mean and std.dev: (429.37, 453.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3971) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.47, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (80, 1007, 3237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9551) [M::mem_pestat] mean and std.dev: (1857.64, 2263.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12708) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (265, 309, 1563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4159) [M::mem_pestat] mean and std.dev: (775.83, 745.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5457) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1234.772 CPU sec, 54.436 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 938215, 36, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 306, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 873) [M::mem_pestat] mean and std.dev: (290.13, 146.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1099) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.31, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (62, 1336, 3831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11369) [M::mem_pestat] mean and std.dev: (1857.50, 1895.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15138) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (322, 1003, 1817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4807) [M::mem_pestat] mean and std.dev: (1345.76, 1254.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6364) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1254.118 CPU sec, 72.499 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 938607, 29, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 449, 797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1837) [M::mem_pestat] mean and std.dev: (475.68, 314.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.36, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (64, 1103, 4450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13222) [M::mem_pestat] mean and std.dev: (2208.38, 2316.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17608) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (310, 382, 1119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2737) [M::mem_pestat] mean and std.dev: (634.29, 481.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3546) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1226.543 CPU sec, 53.936 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938118, 27, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 271, 643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1631) [M::mem_pestat] mean and std.dev: (365.96, 345.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2125) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.32, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (209, 2028, 4450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12932) [M::mem_pestat] mean and std.dev: (2260.78, 2421.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17173) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (261, 486, 2106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5796) [M::mem_pestat] mean and std.dev: (954.50, 961.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7641) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1249.449 CPU sec, 65.707 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938686, 37, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (296, 513, 1657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4379) [M::mem_pestat] mean and std.dev: (879.25, 820.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5740) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.45, 84.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (70, 1113, 3133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9259) [M::mem_pestat] mean and std.dev: (2037.05, 2300.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12322) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1259.829 CPU sec, 72.870 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 939104, 31, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (297, 319, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1176) [M::mem_pestat] mean and std.dev: (373.05, 212.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.28, 84.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (51, 181, 2617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7749) [M::mem_pestat] mean and std.dev: (1382.45, 1796.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10315) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (177, 304, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1110) [M::mem_pestat] mean and std.dev: (326.58, 252.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1421) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1227.974 CPU sec, 54.158 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938654, 39, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (249, 386, 956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2370) [M::mem_pestat] mean and std.dev: (574.43, 496.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3077) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.38, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 677, 2587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7675) [M::mem_pestat] mean and std.dev: (1396.24, 1685.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10219) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (224, 437, 958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2426) [M::mem_pestat] mean and std.dev: (588.08, 494.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3160) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1255.108 CPU sec, 72.727 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 938280, 36, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 341, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1168) [M::mem_pestat] mean and std.dev: (323.00, 201.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.40, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 1099, 4313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12831) [M::mem_pestat] mean and std.dev: (2019.53, 2374.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17090) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (288, 787, 4306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12342) [M::mem_pestat] mean and std.dev: (1887.06, 2372.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16360) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1239.175 CPU sec, 54.498 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938000, 32, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 359, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2371) [M::mem_pestat] mean and std.dev: (499.95, 416.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3074) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.35, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (63, 417, 1862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5460) [M::mem_pestat] mean and std.dev: (1107.81, 1402.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7259) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (223, 504, 716) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1702) [M::mem_pestat] mean and std.dev: (485.06, 362.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2195) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1258.114 CPU sec, 72.606 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 938271, 39, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 387, 1041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2599) [M::mem_pestat] mean and std.dev: (614.04, 526.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3378) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (326, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (110, 650) [M::mem_pestat] mean and std.dev: (380.56, 83.93) [M::mem_pestat] low and high boundaries for proper pairs: (2, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (91, 1003, 2737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8029) [M::mem_pestat] mean and std.dev: (1420.95, 1599.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10675) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (308, 915, 1288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3248) [M::mem_pestat] mean and std.dev: (967.61, 743.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4228) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1232.015 CPU sec, 54.135 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938414, 35, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (145, 274, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000) [M::mem_pestat] mean and std.dev: (282.94, 161.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1285) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.49, 84.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 133, 2682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7934) [M::mem_pestat] mean and std.dev: (1301.32, 1902.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10560) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (209, 334, 1736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4790) [M::mem_pestat] mean and std.dev: (704.92, 999.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1249.335 CPU sec, 68.714 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 937781, 41, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 528, 1444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3754) [M::mem_pestat] mean and std.dev: (711.79, 831.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4909) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.49, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (65, 737, 2644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7802) [M::mem_pestat] mean and std.dev: (1439.41, 1638.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10381) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 521, 1829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5125) [M::mem_pestat] mean and std.dev: (935.20, 1179.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6773) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1249.321 CPU sec, 69.859 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 937847, 29, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 374, 1247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3447) [M::mem_pestat] mean and std.dev: (600.00, 759.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.47, 84.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (205, 1915, 2924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8362) [M::mem_pestat] mean and std.dev: (1687.15, 1524.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11081) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (263, 429, 1114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2816) [M::mem_pestat] mean and std.dev: (716.82, 512.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3667) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1236.433 CPU sec, 54.452 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938326, 45, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 296, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (329.00, 179.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1169) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.25, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (65, 1781, 4464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13262) [M::mem_pestat] mean and std.dev: (2623.53, 2808.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17661) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (342, 819, 1321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3279) [M::mem_pestat] mean and std.dev: (796.30, 567.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4258) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1253.905 CPU sec, 69.330 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (32, 938588, 29, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 345, 806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1888) [M::mem_pestat] mean and std.dev: (423.14, 295.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.36, 84.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (122, 1914, 4460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13136) [M::mem_pestat] mean and std.dev: (2601.24, 2804.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17474) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (256, 899, 1313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3427) [M::mem_pestat] mean and std.dev: (778.36, 522.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4484) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1233.808 CPU sec, 54.284 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 938165, 36, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 312, 808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1846) [M::mem_pestat] mean and std.dev: (450.85, 297.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2365) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.32, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 151, 2481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7377) [M::mem_pestat] mean and std.dev: (987.41, 1410.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9825) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (348, 761, 1300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3204) [M::mem_pestat] mean and std.dev: (803.15, 766.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4156) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1252.919 CPU sec, 70.097 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938913, 42, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (286, 406, 739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1645) [M::mem_pestat] mean and std.dev: (423.82, 249.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2098) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (60, 1952, 3513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10419) [M::mem_pestat] mean and std.dev: (2159.48, 2307.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13872) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1070, 1166, 3741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9083) [M::mem_pestat] mean and std.dev: (1813.00, 1409.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11754) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1228.355 CPU sec, 54.041 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 938732, 36, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 299, 1125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2915) [M::mem_pestat] mean and std.dev: (430.08, 347.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3810) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.24, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 164, 2595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7695) [M::mem_pestat] mean and std.dev: (1134.89, 1579.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10245) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (352, 1110, 1950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5146) [M::mem_pestat] mean and std.dev: (1187.15, 1103.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6744) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1265.379 CPU sec, 73.586 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 939154, 33, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 345, 1368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3656) [M::mem_pestat] mean and std.dev: (674.35, 763.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.54, 84.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (67, 1061, 3013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8905) [M::mem_pestat] mean and std.dev: (1908.97, 2229.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11851) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (302, 1089, 3090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8666) [M::mem_pestat] mean and std.dev: (1843.07, 2526.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11951) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1258.850 CPU sec, 70.257 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 938788, 38, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (318, 329, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (60, 705) [M::mem_pestat] mean and std.dev: (298.08, 100.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.35, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 80, 2147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6367) [M::mem_pestat] mean and std.dev: (788.18, 1478.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8477) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (382, 853, 1114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2578) [M::mem_pestat] mean and std.dev: (862.64, 551.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3310) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1240.161 CPU sec, 54.509 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 938737, 36, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (120, 241, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 849) [M::mem_pestat] mean and std.dev: (236.71, 178.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.49, 84.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (104, 903, 2645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7727) [M::mem_pestat] mean and std.dev: (1601.78, 1795.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10268) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 246, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1392) [M::mem_pestat] mean and std.dev: (340.00, 323.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1796) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1267.437 CPU sec, 74.424 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (28, 938492, 41, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 485, 1053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2669) [M::mem_pestat] mean and std.dev: (546.72, 400.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.30, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 230, 2612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7754) [M::mem_pestat] mean and std.dev: (1357.03, 1716.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10325) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (473, 689, 1211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2687) [M::mem_pestat] mean and std.dev: (878.53, 659.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3516) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.400 CPU sec, 54.323 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 939482, 38, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 403, 700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1522) [M::mem_pestat] mean and std.dev: (452.65, 306.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1933) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.38, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 138, 1184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3522) [M::mem_pestat] mean and std.dev: (415.64, 796.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4691) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 769, 1154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3150) [M::mem_pestat] mean and std.dev: (876.50, 717.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4148) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1256.535 CPU sec, 73.988 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 937606, 33, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (196, 326, 871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2221) [M::mem_pestat] mean and std.dev: (380.38, 295.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (70, 982, 4454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13222) [M::mem_pestat] mean and std.dev: (2239.67, 2558.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17606) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (395, 926, 1927) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4991) [M::mem_pestat] mean and std.dev: (918.08, 725.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6523) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1239.383 CPU sec, 54.537 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938944, 35, 21) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 395, 1265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3355) [M::mem_pestat] mean and std.dev: (638.25, 658.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4400) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.53, 84.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (61, 221, 2640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7798) [M::mem_pestat] mean and std.dev: (1304.59, 1609.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10377) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (254, 586, 1156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2960) [M::mem_pestat] mean and std.dev: (751.30, 639.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3862) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1263.427 CPU sec, 74.807 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 938057, 36, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 354, 1432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3854) [M::mem_pestat] mean and std.dev: (652.38, 609.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5065) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.28, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 537, 2924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8680) [M::mem_pestat] mean and std.dev: (1512.11, 2207.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11558) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (212, 313, 2213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6215) [M::mem_pestat] mean and std.dev: (824.15, 912.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8216) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1243.827 CPU sec, 63.773 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 938010, 54, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (298, 352, 1128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2788) [M::mem_pestat] mean and std.dev: (666.38, 538.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.45, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (79, 449, 2154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6304) [M::mem_pestat] mean and std.dev: (1312.26, 1591.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8379) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (183, 379, 1525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4209) [M::mem_pestat] mean and std.dev: (724.10, 671.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5551) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1236.676 CPU sec, 54.321 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938460, 31, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 392, 784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1806) [M::mem_pestat] mean and std.dev: (467.84, 388.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2317) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (89, 787, 3476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10250) [M::mem_pestat] mean and std.dev: (1590.68, 1846.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13637) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1262.460 CPU sec, 74.326 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938538, 34, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (282, 872, 1685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4491) [M::mem_pestat] mean and std.dev: (931.94, 981.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5894) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (326, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (110, 650) [M::mem_pestat] mean and std.dev: (380.49, 84.00) [M::mem_pestat] low and high boundaries for proper pairs: (2, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 121, 2114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6270) [M::mem_pestat] mean and std.dev: (739.20, 1262.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8348) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (304, 712, 1628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4276) [M::mem_pestat] mean and std.dev: (796.40, 579.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5600) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1233.897 CPU sec, 54.206 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 938553, 36, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 512, 1987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5595) [M::mem_pestat] mean and std.dev: (882.94, 918.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.24, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (112, 2619, 4482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13222) [M::mem_pestat] mean and std.dev: (2534.33, 2712.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17592) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (293, 510, 2445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6749) [M::mem_pestat] mean and std.dev: (1299.17, 1423.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8901) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1258.271 CPU sec, 75.500 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938382, 44, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 339, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1425) [M::mem_pestat] mean and std.dev: (443.71, 326.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.38, 84.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (123, 2441, 4488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13218) [M::mem_pestat] mean and std.dev: (2547.32, 2608.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17583) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (270, 612, 934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2262) [M::mem_pestat] mean and std.dev: (633.91, 472.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2926) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1233.376 CPU sec, 54.295 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 938806, 32, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 387, 764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1816) [M::mem_pestat] mean and std.dev: (432.64, 360.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2342) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.35, 84.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 182, 2912) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8632) [M::mem_pestat] mean and std.dev: (1625.50, 2449.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11492) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (240, 927, 1505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4035) [M::mem_pestat] mean and std.dev: (898.47, 839.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5300) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1257.853 CPU sec, 73.242 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 938907, 36, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 326, 781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1833) [M::mem_pestat] mean and std.dev: (439.39, 333.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2359) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.21, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (83, 963, 3453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10193) [M::mem_pestat] mean and std.dev: (2058.06, 2737.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13563) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (293, 397, 1759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4691) [M::mem_pestat] mean and std.dev: (970.27, 1128.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6157) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1231.443 CPU sec, 54.191 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 937942, 31, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 290, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1250) [M::mem_pestat] mean and std.dev: (339.05, 243.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (61, 240, 3332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9874) [M::mem_pestat] mean and std.dev: (2027.06, 2807.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13258) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (266, 495, 970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2378) [M::mem_pestat] mean and std.dev: (625.43, 517.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3082) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1286.944 CPU sec, 75.848 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 937710, 35, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 375, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1233) [M::mem_pestat] mean and std.dev: (360.96, 248.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1574) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (154, 764, 2937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8503) [M::mem_pestat] mean and std.dev: (1640.24, 1868.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11286) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (382, 652, 1588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4000) [M::mem_pestat] mean and std.dev: (805.13, 679.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5206) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.608 CPU sec, 56.310 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 938778, 28, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (320, 373, 1070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2570) [M::mem_pestat] mean and std.dev: (532.12, 520.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3320) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.48, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 246, 2995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8899) [M::mem_pestat] mean and std.dev: (1816.64, 2581.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12143) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (317, 427, 1262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3152) [M::mem_pestat] mean and std.dev: (648.85, 696.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4097) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1253.641 CPU sec, 70.050 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 938618, 31, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 322, 1291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3435) [M::mem_pestat] mean and std.dev: (449.13, 378.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.41, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (111, 1173, 2907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8499) [M::mem_pestat] mean and std.dev: (1746.32, 1877.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11295) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (274, 396, 1700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4552) [M::mem_pestat] mean and std.dev: (814.28, 720.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5978) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.157 CPU sec, 54.629 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 938330, 33, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 360, 1364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3630) [M::mem_pestat] mean and std.dev: (699.68, 713.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 77, 470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1330) [M::mem_pestat] mean and std.dev: (92.73, 100.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1760) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (302, 866, 1776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4724) [M::mem_pestat] mean and std.dev: (779.67, 624.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6198) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1259.430 CPU sec, 71.406 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938805, 36, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (304, 411, 1611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4225) [M::mem_pestat] mean and std.dev: (836.35, 776.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.36, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 168, 3068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9112) [M::mem_pestat] mean and std.dev: (1610.53, 2250.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12134) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (309, 1196, 2910) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8112) [M::mem_pestat] mean and std.dev: (1770.53, 1911.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10713) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1231.017 CPU sec, 54.224 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 938116, 41, 27) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 339, 872) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2086) [M::mem_pestat] mean and std.dev: (548.91, 471.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.38, 84.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (69, 1034, 4444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13194) [M::mem_pestat] mean and std.dev: (2089.07, 2371.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17569) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (246, 708, 1499) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4005) [M::mem_pestat] mean and std.dev: (828.31, 747.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5258) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1237.741 CPU sec, 59.057 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 939650, 35, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (259, 329, 897) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2173) [M::mem_pestat] mean and std.dev: (523.00, 411.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2811) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.43, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (66, 976, 4498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13362) [M::mem_pestat] mean and std.dev: (2470.17, 2893.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17794) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 2400000 reads in 1263.851 CPU sec, 75.979 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 938501, 35, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (246, 386, 1444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3840) [M::mem_pestat] mean and std.dev: (767.94, 731.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5038) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (167, 2050, 3835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11171) [M::mem_pestat] mean and std.dev: (1999.69, 1758.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14839) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (379, 927, 2288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6106) [M::mem_pestat] mean and std.dev: (1447.25, 1419.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8015) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.415 CPU sec, 54.636 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 938565, 44, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 278, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 918) [M::mem_pestat] mean and std.dev: (316.53, 208.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.19, 84.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 220, 2628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7812) [M::mem_pestat] mean and std.dev: (1372.95, 1928.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10404) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1253.901 CPU sec, 73.078 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 938962, 30, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 257, 724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1724) [M::mem_pestat] mean and std.dev: (393.80, 325.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2224) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (142, 945, 2999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8713) [M::mem_pestat] mean and std.dev: (1793.50, 2070.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11570) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 742, 1046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2682) [M::mem_pestat] mean and std.dev: (763.83, 607.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3500) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1232.511 CPU sec, 54.333 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938370, 41, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 448, 1101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2905) [M::mem_pestat] mean and std.dev: (621.38, 544.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.34, 84.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (69, 243, 3024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8934) [M::mem_pestat] mean and std.dev: (1519.38, 2032.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11889) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 634, 1800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4940) [M::mem_pestat] mean and std.dev: (904.67, 967.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6510) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1254.260 CPU sec, 72.929 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938695, 49, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 343, 640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1416) [M::mem_pestat] mean and std.dev: (388.14, 199.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1804) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.45, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (53, 178, 3112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9230) [M::mem_pestat] mean and std.dev: (1516.20, 2061.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12289) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (235, 419, 1559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4207) [M::mem_pestat] mean and std.dev: (703.94, 694.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5531) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1231.700 CPU sec, 54.201 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 938440, 30, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (243, 252, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (249.75, 88.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 755) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.32, 84.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (69, 1124, 4427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13143) [M::mem_pestat] mean and std.dev: (2366.80, 2889.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17501) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (261, 383, 1181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3021) [M::mem_pestat] mean and std.dev: (679.20, 577.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3941) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1246.671 CPU sec, 63.281 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 937972, 35, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (246, 343, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (272.94, 100.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 942) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.34, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 1253, 2678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7944) [M::mem_pestat] mean and std.dev: (1584.26, 1912.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10577) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (371, 772, 1892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4934) [M::mem_pestat] mean and std.dev: (795.94, 701.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6455) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1273.898 CPU sec, 76.147 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 937921, 26, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 345, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1194) [M::mem_pestat] mean and std.dev: (329.33, 241.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.36, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (102, 1195, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7605) [M::mem_pestat] mean and std.dev: (1551.08, 1709.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10106) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (512, 1045, 1328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2960) [M::mem_pestat] mean and std.dev: (838.78, 482.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3776) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1234.829 CPU sec, 54.910 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 939018, 43, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (203, 287, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082) [M::mem_pestat] mean and std.dev: (286.84, 142.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1375) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (95, 810, 2692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7886) [M::mem_pestat] mean and std.dev: (1399.20, 1644.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10483) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (180, 308, 787) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2001) [M::mem_pestat] mean and std.dev: (401.07, 317.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2608) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1257.658 CPU sec, 73.286 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 938095, 25, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (275, 384, 828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1934) [M::mem_pestat] mean and std.dev: (412.00, 245.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.34, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (78, 519, 3016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8892) [M::mem_pestat] mean and std.dev: (1708.56, 2024.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11830) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (257, 660, 1540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4106) [M::mem_pestat] mean and std.dev: (729.67, 565.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5389) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1236.946 CPU sec, 54.566 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 937981, 48, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 322, 2409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6771) [M::mem_pestat] mean and std.dev: (960.45, 1449.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8952) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.48, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 585, 2986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8862) [M::mem_pestat] mean and std.dev: (1718.79, 2147.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11800) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (220, 723, 3020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8620) [M::mem_pestat] mean and std.dev: (1652.08, 1965.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11420) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1270.289 CPU sec, 73.577 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 937881, 36, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 319, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7344) [M::mem_pestat] mean and std.dev: (1057.37, 1654.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9713) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.57, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (65, 378, 2720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8030) [M::mem_pestat] mean and std.dev: (1300.91, 1625.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10685) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (329, 637, 954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2204) [M::mem_pestat] mean and std.dev: (685.00, 457.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2829) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1257.258 CPU sec, 55.462 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938516, 50, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 369, 614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1392) [M::mem_pestat] mean and std.dev: (327.21, 174.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1781) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.36, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (55, 151, 1255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3655) [M::mem_pestat] mean and std.dev: (579.98, 879.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4855) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (248, 911, 1871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5117) [M::mem_pestat] mean and std.dev: (942.22, 800.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6740) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1256.150 CPU sec, 68.004 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 938121, 31, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 348, 734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1754) [M::mem_pestat] mean and std.dev: (511.36, 434.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2264) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.36, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (58, 374, 3128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9268) [M::mem_pestat] mean and std.dev: (1905.94, 2587.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12338) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 270, 1036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2796) [M::mem_pestat] mean and std.dev: (530.68, 537.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3676) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1269.877 CPU sec, 73.988 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 937472, 33, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (173, 279, 568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1358) [M::mem_pestat] mean and std.dev: (398.96, 329.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1753) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.34, 84.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 129, 4450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13268) [M::mem_pestat] mean and std.dev: (2065.82, 3095.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17677) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (276, 1084, 3406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9666) [M::mem_pestat] mean and std.dev: (1851.76, 1929.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12796) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1246.953 CPU sec, 54.900 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 938410, 32, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (280, 392, 1326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3418) [M::mem_pestat] mean and std.dev: (625.55, 548.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.28, 84.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 110, 3374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10068) [M::mem_pestat] mean and std.dev: (1421.25, 2129.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13415) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (307, 682, 1373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3505) [M::mem_pestat] mean and std.dev: (819.33, 746.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4571) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1252.605 CPU sec, 61.405 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 938921, 35, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 289, 609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1367) [M::mem_pestat] mean and std.dev: (386.79, 265.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1746) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.47, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 139, 2970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8822) [M::mem_pestat] mean and std.dev: (1453.97, 1724.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11748) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (847, 1054, 2115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4651) [M::mem_pestat] mean and std.dev: (1208.43, 711.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5919) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1240.676 CPU sec, 54.494 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 939095, 33, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (130, 319, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2746) [M::mem_pestat] mean and std.dev: (495.35, 519.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3618) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (60, 529, 4395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13065) [M::mem_pestat] mean and std.dev: (2171.58, 2470.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17400) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (551, 1095, 1977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4829) [M::mem_pestat] mean and std.dev: (1047.12, 737.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6255) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1259.245 CPU sec, 72.182 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 938145, 34, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 367, 1229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3161) [M::mem_pestat] mean and std.dev: (609.52, 574.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4127) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.33, 84.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 253, 4326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12908) [M::mem_pestat] mean and std.dev: (1968.59, 2402.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17199) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (329, 1001, 1456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3710) [M::mem_pestat] mean and std.dev: (809.56, 566.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4837) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1232.548 CPU sec, 54.282 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 937938, 32, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 392, 1168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2938) [M::mem_pestat] mean and std.dev: (688.44, 737.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.57, 84.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (194, 862, 2585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7367) [M::mem_pestat] mean and std.dev: (1409.60, 1555.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9758) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 437, 954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2454) [M::mem_pestat] mean and std.dev: (495.28, 357.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3204) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1269.499 CPU sec, 72.188 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 938458, 45, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 352, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029) [M::mem_pestat] mean and std.dev: (328.00, 159.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1288) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.51, 84.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (101, 2007, 5254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15560) [M::mem_pestat] mean and std.dev: (2830.49, 2869.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20713) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (712, 1109, 1673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3595) [M::mem_pestat] mean and std.dev: (904.70, 487.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4556) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1254.437 CPU sec, 66.892 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938285, 33, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 368, 984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2470) [M::mem_pestat] mean and std.dev: (598.35, 666.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3266) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.41, 84.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (91, 1821, 3124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9190) [M::mem_pestat] mean and std.dev: (1895.06, 2033.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12223) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (282, 367, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2079) [M::mem_pestat] mean and std.dev: (456.15, 326.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2678) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.413 CPU sec, 54.304 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 938887, 27, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 321, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (74, 574) [M::mem_pestat] mean and std.dev: (289.84, 83.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 577, 4444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13260) [M::mem_pestat] mean and std.dev: (2083.07, 2403.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17668) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (208, 813, 2103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5893) [M::mem_pestat] mean and std.dev: (1139.21, 1403.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7788) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1273.446 CPU sec, 73.986 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938968, 32, 22) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 335, 1041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2689) [M::mem_pestat] mean and std.dev: (502.10, 451.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.49, 84.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (72, 194, 1892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5532) [M::mem_pestat] mean and std.dev: (656.34, 1095.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7352) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 648, 1042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2692) [M::mem_pestat] mean and std.dev: (675.81, 574.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3517) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.981 CPU sec, 54.356 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 938501, 39, 20) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (254, 329, 614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1334) [M::mem_pestat] mean and std.dev: (380.47, 265.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1694) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (326, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (110, 650) [M::mem_pestat] mean and std.dev: (380.66, 84.10) [M::mem_pestat] low and high boundaries for proper pairs: (2, 758) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (58, 325, 4443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13213) [M::mem_pestat] mean and std.dev: (2171.67, 2737.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17598) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (230, 470, 1812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4976) [M::mem_pestat] mean and std.dev: (1060.90, 1121.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6558) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1259.146 CPU sec, 72.674 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 938266, 34, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (280, 365, 1052) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2596) [M::mem_pestat] mean and std.dev: (612.00, 560.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.36, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (71, 518, 4502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13364) [M::mem_pestat] mean and std.dev: (2174.44, 2857.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17795) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (305, 407, 1058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2564) [M::mem_pestat] mean and std.dev: (556.33, 362.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1239.883 CPU sec, 54.539 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 937811, 36, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (260, 343, 672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1496) [M::mem_pestat] mean and std.dev: (382.00, 264.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1908) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.35, 84.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (77, 556, 2967) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8747) [M::mem_pestat] mean and std.dev: (1667.31, 2177.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11637) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (312, 780, 1197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2967) [M::mem_pestat] mean and std.dev: (717.31, 410.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3852) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1271.492 CPU sec, 75.688 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (23, 938720, 42, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 276, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 724) [M::mem_pestat] mean and std.dev: (275.19, 139.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 898) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.52, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 812, 2617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7739) [M::mem_pestat] mean and std.dev: (1485.10, 1792.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10300) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (261, 745, 1014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2520) [M::mem_pestat] mean and std.dev: (722.00, 630.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3273) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1236.912 CPU sec, 58.805 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938220, 44, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 356, 693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1513) [M::mem_pestat] mean and std.dev: (502.12, 392.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2074) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.38, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (67, 1323, 2656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7834) [M::mem_pestat] mean and std.dev: (1641.93, 1689.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10423) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (126, 657, 1319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3705) [M::mem_pestat] mean and std.dev: (659.08, 563.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4898) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1235.001 CPU sec, 54.253 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 937468, 35, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 518, 1842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5080) [M::mem_pestat] mean and std.dev: (898.57, 879.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.48, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (91, 1287, 2981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8761) [M::mem_pestat] mean and std.dev: (1837.15, 2178.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11651) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (269, 411, 1095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2747) [M::mem_pestat] mean and std.dev: (637.17, 643.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3573) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1270.024 CPU sec, 73.211 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 937991, 43, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (298, 346, 1481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3847) [M::mem_pestat] mean and std.dev: (1017.00, 1084.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5355) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.45, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 81, 3018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8980) [M::mem_pestat] mean and std.dev: (1478.74, 2223.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11961) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (355, 444, 1557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3961) [M::mem_pestat] mean and std.dev: (623.94, 481.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5163) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1239.181 CPU sec, 54.477 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 938718, 38, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 318, 429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 807) [M::mem_pestat] mean and std.dev: (260.67, 113.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 996) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.48, 84.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 217, 1777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5239) [M::mem_pestat] mean and std.dev: (967.29, 1132.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6970) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (236, 1099, 1781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4871) [M::mem_pestat] mean and std.dev: (981.00, 809.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6416) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1257.450 CPU sec, 73.109 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (26, 939349, 33, 23) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 288, 797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1949) [M::mem_pestat] mean and std.dev: (456.50, 423.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 217, 3033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8999) [M::mem_pestat] mean and std.dev: (1550.55, 1968.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11982) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (278, 693, 1140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2864) [M::mem_pestat] mean and std.dev: (719.36, 605.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3726) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1230.972 CPU sec, 54.091 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938524, 28, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 335, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (316.43, 200.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.36, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (113, 1390, 2678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7808) [M::mem_pestat] mean and std.dev: (1868.52, 2019.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10373) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (252, 465, 1154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2958) [M::mem_pestat] mean and std.dev: (702.55, 702.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3860) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1263.225 CPU sec, 75.368 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 938643, 41, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 349, 674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1560) [M::mem_pestat] mean and std.dev: (395.41, 255.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2003) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.28, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 210, 2688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7956) [M::mem_pestat] mean and std.dev: (1275.08, 2032.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10590) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (365, 864, 1050) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2420) [M::mem_pestat] mean and std.dev: (694.93, 395.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3105) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1238.637 CPU sec, 54.592 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 938385, 40, 24) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 359, 835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1959) [M::mem_pestat] mean and std.dev: (437.94, 301.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 635, 3261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9707) [M::mem_pestat] mean and std.dev: (1678.53, 1992.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12930) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (339, 794, 1792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4698) [M::mem_pestat] mean and std.dev: (860.77, 689.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6151) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1270.261 CPU sec, 74.749 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (25, 937511, 39, 17) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 322, 720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1688) [M::mem_pestat] mean and std.dev: (349.62, 239.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2172) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.28, 84.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (60, 438, 4348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12924) [M::mem_pestat] mean and std.dev: (2208.28, 2878.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17212) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (322, 1113, 2003) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5365) [M::mem_pestat] mean and std.dev: (1112.75, 927.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7046) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1232.426 CPU sec, 56.149 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 938935, 31, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 318, 1104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2902) [M::mem_pestat] mean and std.dev: (620.64, 705.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3801) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (61, 178, 3738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11092) [M::mem_pestat] mean and std.dev: (1666.58, 1913.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14769) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 571, 3135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8927) [M::mem_pestat] mean and std.dev: (1715.00, 2256.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11823) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1253.507 CPU sec, 72.469 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 938992, 31, 18) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 302, 1037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2629) [M::mem_pestat] mean and std.dev: (553.44, 487.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3425) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.58, 84.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (58, 216, 3064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9076) [M::mem_pestat] mean and std.dev: (1394.23, 1748.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12082) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (283, 778, 2143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5863) [M::mem_pestat] mean and std.dev: (660.71, 598.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7723) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1231.050 CPU sec, 54.467 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 938399, 33, 25) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 272, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (234.33, 106.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.27, 84.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 388, 2966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8804) [M::mem_pestat] mean and std.dev: (1739.45, 2304.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11723) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (239, 331, 1292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3398) [M::mem_pestat] mean and std.dev: (741.04, 791.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4451) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1255.901 CPU sec, 72.794 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 939089, 30, 19) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 281, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (229.91, 92.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.29, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 147, 773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2207) [M::mem_pestat] mean and std.dev: (296.46, 445.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2924) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (434, 1397, 2522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6698) [M::mem_pestat] mean and std.dev: (1671.56, 1513.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8786) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1230.368 CPU sec, 54.207 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938278, 28, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 345, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1189) [M::mem_pestat] mean and std.dev: (360.30, 251.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.37, 84.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 830, 4455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13287) [M::mem_pestat] mean and std.dev: (2186.43, 2649.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17703) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (335, 1178, 4025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11405) [M::mem_pestat] mean and std.dev: (1953.09, 1982.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1234.930 CPU sec, 56.606 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 2400000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 937785, 35, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 401, 1023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2459) [M::mem_pestat] mean and std.dev: (561.91, 411.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3177) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.39, 84.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (80, 554, 4473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13259) [M::mem_pestat] mean and std.dev: (2426.97, 3051.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17652) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (204, 662, 6344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18624) [M::mem_pestat] mean and std.dev: (2352.25, 3206.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 24764) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1271.386 CPU sec, 74.727 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (24, 937629, 37, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (254, 348, 832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1988) [M::mem_pestat] mean and std.dev: (476.26, 331.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.45, 84.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (62, 144, 2584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7628) [M::mem_pestat] mean and std.dev: (928.73, 1532.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10150) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (815, 1113, 2210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5000) [M::mem_pestat] mean and std.dev: (1204.83, 795.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6395) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1241.843 CPU sec, 56.571 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (22, 938773, 27, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 345, 823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1989) [M::mem_pestat] mean and std.dev: (567.10, 519.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2643) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.52, 84.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (68, 592, 1987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5825) [M::mem_pestat] mean and std.dev: (1094.15, 1230.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7744) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 422, 1334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3678) [M::mem_pestat] mean and std.dev: (811.79, 845.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4850) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1248.024 CPU sec, 71.100 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 937949, 43, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 344, 555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1161) [M::mem_pestat] mean and std.dev: (362.89, 195.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.26, 84.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (70, 349, 4426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13138) [M::mem_pestat] mean and std.dev: (2058.33, 2689.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17494) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (210, 340, 960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2460) [M::mem_pestat] mean and std.dev: (435.33, 324.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3210) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1229.033 CPU sec, 54.323 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 937747, 33, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 400, 1127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2893) [M::mem_pestat] mean and std.dev: (539.35, 503.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3776) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.33, 84.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 204, 2158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6410) [M::mem_pestat] mean and std.dev: (853.87, 1123.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8536) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (253, 653, 3141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8917) [M::mem_pestat] mean and std.dev: (1505.67, 1592.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11805) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 2400000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 2400000 reads in 1257.674 CPU sec, 74.302 real sec [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (27, 938712, 52, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 362, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1094) [M::mem_pestat] mean and std.dev: (380.38, 264.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1438) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.44, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (77, 1118, 3572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10562) [M::mem_pestat] mean and std.dev: (2163.56, 2670.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14057) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (417, 861, 2070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5376) [M::mem_pestat] mean and std.dev: (969.09, 799.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7029) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1229.013 CPU sec, 54.095 real sec [M::process] read 2400000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 2400000 paired-end sequences [M::process] read 733620 sequences (73362000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (30, 938674, 34, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 364, 1250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3238) [M::mem_pestat] mean and std.dev: (545.23, 549.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4232) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.42, 84.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (58, 214, 1924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5656) [M::mem_pestat] mean and std.dev: (972.91, 1378.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7522) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (278, 800, 1705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4559) [M::mem_pestat] mean and std.dev: (996.23, 1044.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5986) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 2400000 reads in 1233.561 CPU sec, 59.464 real sec [M::process] 0 single-end sequences; 733620 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 286730, 18, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 336, 1953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5523) [M::mem_pestat] mean and std.dev: (1190.50, 1489.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7308) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (325, 377, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (107, 652) [M::mem_pestat] mean and std.dev: (380.52, 83.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (211, 2567, 4460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12958) [M::mem_pestat] mean and std.dev: (2689.61, 2768.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17207) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 733620 reads in 387.741 CPU sec, 17.054 real sec [main] Version: 0.7.17-r1188 [main] CMD: /home/csyk2proj/tools/bwa.kit/bwa mem -p -t24 -R@RG\tID:KPGP-00256\tPL:ILLUMINA\tSM:KPGP-00256 -C /home/csyk2proj/data/bwa.kit/hs38DH.fa - [main] Real time: 56947.227 sec; CPU: 667672.965 sec