[M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::process] read 1925986 sequences (240000204 bp)... [M::process] 0 single-end sequences; 1925986 paired-end sequences [M::process] read 1925966 sequences (240000150 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1263, 752182, 48, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 110, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (125.16, 73.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.21) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 89, 2965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8837) [M::mem_pestat] mean and std.dev: (1357.27, 2020.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11773) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (81, 230, 1347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3879) [M::mem_pestat] mean and std.dev: (599.82, 716.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5145) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925986 reads in 1024.619 CPU sec, 63.332 real sec [M::process] 0 single-end sequences; 1925966 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1302, 751637, 49, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 112, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (124.68, 71.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 110, 2654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7916) [M::mem_pestat] mean and std.dev: (1137.60, 2027.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10547) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (123, 183, 1037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2865) [M::mem_pestat] mean and std.dev: (495.18, 585.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3779) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925966 reads in 963.768 CPU sec, 44.234 real sec [M::process] read 1925878 sequences (240000174 bp)... [M::process] 0 single-end sequences; 1925878 paired-end sequences [M::process] read 1925906 sequences (240000190 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1319, 751571, 62, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 110, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (124.30, 72.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 438, 4373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13053) [M::mem_pestat] mean and std.dev: (1765.89, 2132.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17393) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (143, 240, 1104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3026) [M::mem_pestat] mean and std.dev: (599.23, 624.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3987) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925878 reads in 990.750 CPU sec, 64.553 real sec [M::process] 0 single-end sequences; 1925906 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1220, 751100, 61, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 107, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (123.38, 72.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 481, 3069) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9113) [M::mem_pestat] mean and std.dev: (1737.77, 2296.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12135) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (71, 133, 889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2525) [M::mem_pestat] mean and std.dev: (462.52, 602.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3343) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925906 reads in 962.421 CPU sec, 42.495 real sec [M::process] read 1925828 sequences (240000072 bp)... [M::process] 0 single-end sequences; 1925828 paired-end sequences [M::process] read 1925940 sequences (240000174 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1170, 752249, 46, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 114, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (126.73, 71.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 176, 2135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6341) [M::mem_pestat] mean and std.dev: (1193.24, 1680.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8444) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (80, 192, 684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1892) [M::mem_pestat] mean and std.dev: (310.56, 407.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2496) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925828 reads in 994.190 CPU sec, 63.161 real sec [M::process] 0 single-end sequences; 1925940 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1239, 752540, 54, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 112, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (125.34, 73.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 292, 1922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5698) [M::mem_pestat] mean and std.dev: (1026.44, 1334.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7586) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (87, 161, 913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2565) [M::mem_pestat] mean and std.dev: (340.66, 480.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3391) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925940 reads in 959.684 CPU sec, 42.250 real sec [M::process] read 1925894 sequences (240000220 bp)... [M::process] 0 single-end sequences; 1925894 paired-end sequences [M::process] read 1925918 sequences (240000102 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1233, 751559, 44, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 110, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (124.08, 73.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.42, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 87, 1679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4991) [M::mem_pestat] mean and std.dev: (880.05, 1443.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6652) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (124, 249, 1403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3961) [M::mem_pestat] mean and std.dev: (546.66, 606.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5240) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925894 reads in 984.220 CPU sec, 63.510 real sec [M::process] 0 single-end sequences; 1925918 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1283, 752740, 49, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 110, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (121.86, 71.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 133, 1557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4599) [M::mem_pestat] mean and std.dev: (899.32, 1431.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6625) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (60, 163, 673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1899) [M::mem_pestat] mean and std.dev: (414.00, 557.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2645) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925918 reads in 958.828 CPU sec, 42.184 real sec [M::process] read 1925940 sequences (240000100 bp)... [M::process] 0 single-end sequences; 1925940 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1248, 751860, 61, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 114, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (125.62, 72.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 153, 3230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9652) [M::mem_pestat] mean and std.dev: (1335.65, 2125.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12863) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (114, 242, 1232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3468) [M::mem_pestat] mean and std.dev: (667.91, 873.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4586) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925940 reads in 976.356 CPU sec, 55.352 real sec [M::process] read 1925940 sequences (240000152 bp)... [M::process] 0 single-end sequences; 1925940 paired-end sequences [M::process] read 1926020 sequences (240000028 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1195, 751583, 53, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 105, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (121.83, 71.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.43, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 130, 1360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4040) [M::mem_pestat] mean and std.dev: (463.26, 842.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5380) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (103, 197, 878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2428) [M::mem_pestat] mean and std.dev: (460.72, 537.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3203) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925940 reads in 1005.845 CPU sec, 62.590 real sec [M::process] 0 single-end sequences; 1926020 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1219, 751953, 49, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 110, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (127.43, 74.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (51, 114, 1914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5640) [M::mem_pestat] mean and std.dev: (749.53, 1209.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7503) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (109, 197, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 760) [M::mem_pestat] mean and std.dev: (168.79, 122.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 977) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1926020 reads in 961.730 CPU sec, 43.509 real sec [M::process] read 1925896 sequences (240000094 bp)... [M::process] 0 single-end sequences; 1925896 paired-end sequences [M::process] read 1925964 sequences (240000140 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1296, 751505, 47, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 107, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (125.45, 75.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 403, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.45, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 77, 1698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5040) [M::mem_pestat] mean and std.dev: (977.36, 1592.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7349) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (92, 194, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (170.28, 110.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925896 reads in 1002.546 CPU sec, 63.141 real sec [M::process] 0 single-end sequences; 1925964 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1296, 751326, 59, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 113, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (124.77, 72.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.42, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 187, 2168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6480) [M::mem_pestat] mean and std.dev: (1187.70, 1689.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8636) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (122, 198, 645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1691) [M::mem_pestat] mean and std.dev: (354.13, 429.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2214) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925964 reads in 960.741 CPU sec, 42.700 real sec [M::process] read 1925772 sequences (240000152 bp)... [M::process] 0 single-end sequences; 1925772 paired-end sequences [M::process] read 1925936 sequences (240000020 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1239, 752208, 49, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 105, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (121.37, 70.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.28, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 120, 1343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3971) [M::mem_pestat] mean and std.dev: (500.37, 901.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5285) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (75, 275, 1061) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3033) [M::mem_pestat] mean and std.dev: (629.71, 796.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4019) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925772 reads in 979.159 CPU sec, 63.173 real sec [M::process] 0 single-end sequences; 1925936 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1272, 751828, 52, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 106, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (123.24, 73.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 63, 1906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5666) [M::mem_pestat] mean and std.dev: (886.90, 1533.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7546) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (110, 211, 775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2105) [M::mem_pestat] mean and std.dev: (416.91, 432.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2770) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925936 reads in 955.964 CPU sec, 42.058 real sec [M::process] read 1925964 sequences (240000030 bp)... [M::process] 0 single-end sequences; 1925964 paired-end sequences [M::process] read 1925858 sequences (240000054 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1295, 751163, 54, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 112, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (126.46, 73.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.33, 30.21) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 136, 1827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5445) [M::mem_pestat] mean and std.dev: (823.04, 1297.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7254) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (101, 232, 759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2075) [M::mem_pestat] mean and std.dev: (437.86, 515.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2733) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925964 reads in 1008.376 CPU sec, 65.045 real sec [M::process] 0 single-end sequences; 1925858 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1269, 752007, 42, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 111, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (125.33, 72.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 49, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1070) [M::mem_pestat] mean and std.dev: (71.16, 99.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1421) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (117, 176, 754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2028) [M::mem_pestat] mean and std.dev: (373.00, 527.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2665) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925858 reads in 961.748 CPU sec, 42.276 real sec [M::process] read 1925954 sequences (240000084 bp)... [M::process] 0 single-end sequences; 1925954 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1181, 752041, 51, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 113, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (127.70, 75.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 184, 1859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5515) [M::mem_pestat] mean and std.dev: (1174.84, 1644.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7753) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 263, 1227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3373) [M::mem_pestat] mean and std.dev: (627.89, 710.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4446) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925954 reads in 995.417 CPU sec, 63.388 real sec [M::process] read 1925908 sequences (240000246 bp)... [M::process] 0 single-end sequences; 1925908 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1244, 751774, 39, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 112, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (126.81, 74.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 130, 1015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2973) [M::mem_pestat] mean and std.dev: (206.84, 405.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3952) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (113, 195, 851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2327) [M::mem_pestat] mean and std.dev: (318.38, 432.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3065) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1925874 sequences (240000018 bp)... [M::mem_process_seqs] Processed 1925908 reads in 992.794 CPU sec, 63.666 real sec [M::process] 0 single-end sequences; 1925874 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1248, 752872, 44, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 106, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (120.91, 71.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.32, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 194, 3674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10952) [M::mem_pestat] mean and std.dev: (1458.20, 2120.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14591) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (86, 155, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1358) [M::mem_pestat] mean and std.dev: (268.53, 317.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1782) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925874 reads in 947.753 CPU sec, 41.836 real sec [M::process] read 1925914 sequences (240000058 bp)... [M::process] 0 single-end sequences; 1925914 paired-end sequences [M::process] read 1925930 sequences (240000198 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1282, 751801, 46, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 106, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (122.67, 71.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 448, 3641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10819) [M::mem_pestat] mean and std.dev: (1722.43, 2134.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14408) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (121, 181, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1282) [M::mem_pestat] mean and std.dev: (227.53, 225.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1669) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925914 reads in 989.828 CPU sec, 63.554 real sec [M::process] 0 single-end sequences; 1925930 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1250, 752174, 49, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 115, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (126.82, 71.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.42, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 120, 1015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3001) [M::mem_pestat] mean and std.dev: (389.31, 685.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3994) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 263, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3165) [M::mem_pestat] mean and std.dev: (422.57, 431.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4169) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925930 reads in 959.052 CPU sec, 42.228 real sec [M::process] read 1925864 sequences (240000124 bp)... [M::process] 0 single-end sequences; 1925864 paired-end sequences [M::process] read 1926026 sequences (240000090 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1273, 752467, 47, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 111, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (123.95, 72.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 191, 3574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10656) [M::mem_pestat] mean and std.dev: (1630.70, 2111.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14197) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (112, 225, 698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1870) [M::mem_pestat] mean and std.dev: (389.27, 441.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2456) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925864 reads in 988.691 CPU sec, 64.845 real sec [M::process] 0 single-end sequences; 1926026 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1241, 752081, 45, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 114, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (128.94, 73.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.46, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (60, 300, 3214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9522) [M::mem_pestat] mean and std.dev: (1647.27, 2238.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12676) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (117, 175, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 897) [M::mem_pestat] mean and std.dev: (172.08, 125.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1157) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1926026 reads in 956.013 CPU sec, 41.977 real sec [M::process] read 1925942 sequences (240000032 bp)... [M::process] 0 single-end sequences; 1925942 paired-end sequences [M::process] read 1925848 sequences (240000098 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1316, 751827, 42, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 112, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (125.40, 73.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 316, 2685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8001) [M::mem_pestat] mean and std.dev: (1525.14, 2030.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10659) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (117, 263, 1293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3645) [M::mem_pestat] mean and std.dev: (531.00, 622.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4821) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925942 reads in 999.313 CPU sec, 63.809 real sec [M::process] 0 single-end sequences; 1925848 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1231, 751900, 47, 65) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 112, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (126.24, 73.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.42, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 188, 4375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13051) [M::mem_pestat] mean and std.dev: (1728.87, 2197.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17389) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (89, 190, 658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1796) [M::mem_pestat] mean and std.dev: (306.22, 374.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2365) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925848 reads in 955.346 CPU sec, 41.978 real sec [M::process] read 1925854 sequences (240000242 bp)... [M::process] 0 single-end sequences; 1925854 paired-end sequences [M::process] read 1925782 sequences (240000052 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1278, 751374, 37, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 112, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (127.40, 73.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (54, 185, 4344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12924) [M::mem_pestat] mean and std.dev: (1836.68, 2520.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17214) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (90, 159, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (137.45, 64.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925854 reads in 994.470 CPU sec, 64.527 real sec [M::process] 0 single-end sequences; 1925782 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1232, 751890, 43, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 109, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (121.80, 71.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 121, 2799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8321) [M::mem_pestat] mean and std.dev: (1271.33, 1726.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11082) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (105, 202, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (181.93, 143.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1033) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925782 reads in 953.499 CPU sec, 41.883 real sec [M::process] read 1925832 sequences (240000104 bp)... [M::process] 0 single-end sequences; 1925832 paired-end sequences [M::process] read 1925872 sequences (240000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1222, 751970, 38, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 105, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (121.74, 72.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 139, 2678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7998) [M::mem_pestat] mean and std.dev: (1410.84, 1931.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10658) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (125, 204, 389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (179.88, 120.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925832 reads in 1001.483 CPU sec, 63.199 real sec [M::process] 0 single-end sequences; 1925872 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1221, 752274, 33, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 112, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (124.92, 71.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 76, 987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2917) [M::mem_pestat] mean and std.dev: (253.52, 480.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3882) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (120, 192, 726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1938) [M::mem_pestat] mean and std.dev: (405.75, 488.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2544) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925872 reads in 951.846 CPU sec, 43.232 real sec [M::process] read 1925862 sequences (240000060 bp)... [M::process] 0 single-end sequences; 1925862 paired-end sequences [M::process] read 1925870 sequences (240000184 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1245, 751617, 53, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 104, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (121.84, 71.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 159, 2619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7807) [M::mem_pestat] mean and std.dev: (1212.26, 1676.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10401) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 231, 964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2634) [M::mem_pestat] mean and std.dev: (462.65, 526.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3469) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925862 reads in 983.280 CPU sec, 64.132 real sec [M::process] 0 single-end sequences; 1925870 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1231, 752005, 56, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 115, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (127.30, 73.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 118, 2507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7463) [M::mem_pestat] mean and std.dev: (1264.77, 1795.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9941) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (75, 182, 913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2589) [M::mem_pestat] mean and std.dev: (504.83, 671.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3427) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925870 reads in 950.185 CPU sec, 41.900 real sec [M::process] read 1925918 sequences (240000054 bp)... [M::process] 0 single-end sequences; 1925918 paired-end sequences [M::process] read 1925928 sequences (240000044 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1208, 752358, 56, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 116, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (127.70, 74.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.46, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 151, 2575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7661) [M::mem_pestat] mean and std.dev: (1243.24, 1742.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10204) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (91, 215, 1010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2848) [M::mem_pestat] mean and std.dev: (531.33, 685.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3767) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925918 reads in 1001.389 CPU sec, 63.472 real sec [M::process] 0 single-end sequences; 1925928 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1274, 752676, 62, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 113, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (125.62, 72.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 107, 2710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8072) [M::mem_pestat] mean and std.dev: (1132.60, 1635.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10753) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 207, 645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1657) [M::mem_pestat] mean and std.dev: (306.26, 322.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2163) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925928 reads in 959.034 CPU sec, 42.174 real sec [M::process] read 1926036 sequences (240000042 bp)... [M::process] 0 single-end sequences; 1926036 paired-end sequences [M::process] read 1925936 sequences (240000062 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1335, 751403, 56, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 111, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (125.24, 74.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 225, 3705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11029) [M::mem_pestat] mean and std.dev: (1579.20, 1960.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14691) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (119, 192, 297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 653) [M::mem_pestat] mean and std.dev: (163.41, 76.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 831) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1926036 reads in 996.897 CPU sec, 63.500 real sec [M::process] 0 single-end sequences; 1925936 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1298, 751759, 46, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 111, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (125.82, 74.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.45, 30.06) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 152, 1143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3383) [M::mem_pestat] mean and std.dev: (477.10, 845.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4503) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (114, 190, 242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (160.93, 74.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 626) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925936 reads in 945.793 CPU sec, 41.590 real sec [M::process] read 1925954 sequences (240000102 bp)... [M::process] 0 single-end sequences; 1925954 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1262, 752041, 58, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 111, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (123.25, 71.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 321, 1865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5547) [M::mem_pestat] mean and std.dev: (1147.46, 1547.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7388) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (103, 195, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 937) [M::mem_pestat] mean and std.dev: (176.63, 144.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1215) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925954 reads in 986.548 CPU sec, 59.222 real sec [M::process] read 1925906 sequences (240000130 bp)... [M::process] 0 single-end sequences; 1925906 paired-end sequences [M::process] read 1925956 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1232, 752217, 40, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 108, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (123.50, 73.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.31, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 149, 1030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3018) [M::mem_pestat] mean and std.dev: (328.83, 529.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4012) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (128, 395, 1465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4139) [M::mem_pestat] mean and std.dev: (684.24, 760.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5476) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925906 reads in 1007.462 CPU sec, 62.814 real sec [M::process] 0 single-end sequences; 1925956 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1234, 752263, 50, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 108, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (125.27, 72.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 195, 1916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5686) [M::mem_pestat] mean and std.dev: (969.02, 1446.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7571) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (111, 173, 721) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1941) [M::mem_pestat] mean and std.dev: (311.75, 340.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2551) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925956 reads in 961.945 CPU sec, 43.628 real sec [M::process] read 1925934 sequences (240000070 bp)... [M::process] 0 single-end sequences; 1925934 paired-end sequences [M::process] read 1925940 sequences (240000236 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1257, 751873, 43, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 107, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (122.17, 72.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 147, 1771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5251) [M::mem_pestat] mean and std.dev: (1096.56, 1615.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7557) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (95, 185, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (157.53, 86.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 811) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925934 reads in 999.923 CPU sec, 63.434 real sec [M::process] 0 single-end sequences; 1925940 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1304, 751920, 29, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 115, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (128.26, 74.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.11) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 63, 1648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4880) [M::mem_pestat] mean and std.dev: (981.79, 1583.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7315) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (184, 616, 1313) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3571) [M::mem_pestat] mean and std.dev: (722.57, 663.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4700) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925940 reads in 948.654 CPU sec, 41.972 real sec [M::process] read 1925890 sequences (240000240 bp)... [M::process] 0 single-end sequences; 1925890 paired-end sequences [M::process] read 1925892 sequences (240000128 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1233, 751542, 48, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 111, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (127.06, 75.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.21) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 132, 2664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7946) [M::mem_pestat] mean and std.dev: (1242.74, 1837.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10587) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 241, 1046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2858) [M::mem_pestat] mean and std.dev: (611.74, 760.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3764) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925890 reads in 1002.202 CPU sec, 63.800 real sec [M::process] 0 single-end sequences; 1925892 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1175, 751628, 41, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 111, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (123.88, 71.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 295, 1929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5725) [M::mem_pestat] mean and std.dev: (1039.85, 1509.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7623) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 230, 944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2552) [M::mem_pestat] mean and std.dev: (484.54, 527.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3356) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925892 reads in 961.233 CPU sec, 42.327 real sec [M::process] read 1925978 sequences (240000162 bp)... [M::process] 0 single-end sequences; 1925978 paired-end sequences [M::process] read 1925966 sequences (240000236 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1232, 752957, 41, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 106, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (124.07, 73.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 317, 4387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13131) [M::mem_pestat] mean and std.dev: (1876.20, 2395.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17503) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (109, 215, 1063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2971) [M::mem_pestat] mean and std.dev: (616.66, 743.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3925) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925978 reads in 981.350 CPU sec, 64.359 real sec [M::process] 0 single-end sequences; 1925966 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1260, 751562, 41, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 114, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (128.37, 73.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.31, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 116, 2649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7915) [M::mem_pestat] mean and std.dev: (1150.34, 1700.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10548) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (42, 127, 1120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3276) [M::mem_pestat] mean and std.dev: (567.41, 861.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4354) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925966 reads in 961.384 CPU sec, 42.252 real sec [M::process] read 1925986 sequences (240000048 bp)... [M::process] 0 single-end sequences; 1925986 paired-end sequences [M::process] read 1925838 sequences (240000180 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1261, 752626, 52, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 112, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (125.21, 72.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 200, 4395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13073) [M::mem_pestat] mean and std.dev: (1800.13, 2234.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17412) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (72, 192, 1588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4620) [M::mem_pestat] mean and std.dev: (798.02, 1157.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6136) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925986 reads in 993.360 CPU sec, 64.806 real sec [M::process] 0 single-end sequences; 1925838 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1287, 751960, 44, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 107, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (123.61, 72.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 178, 4378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13034) [M::mem_pestat] mean and std.dev: (1620.41, 2204.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17362) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (124, 310, 984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2704) [M::mem_pestat] mean and std.dev: (510.57, 517.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3564) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925838 reads in 957.913 CPU sec, 42.177 real sec [M::process] read 1925970 sequences (240000008 bp)... [M::process] 0 single-end sequences; 1925970 paired-end sequences [M::process] read 1925988 sequences (240000196 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1286, 751494, 53, 64) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 109, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (124.28, 74.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 59, 1036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3078) [M::mem_pestat] mean and std.dev: (261.53, 602.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4099) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (79, 246, 1004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2854) [M::mem_pestat] mean and std.dev: (501.66, 648.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3779) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925970 reads in 1009.247 CPU sec, 62.862 real sec [M::process] 0 single-end sequences; 1925988 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1225, 752127, 48, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 110, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (125.48, 75.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.44, 30.08) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 42, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 951) [M::mem_pestat] mean and std.dev: (98.38, 160.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1264) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (100, 185, 514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1342) [M::mem_pestat] mean and std.dev: (244.62, 275.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1756) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925988 reads in 962.474 CPU sec, 43.603 real sec [M::process] read 1925938 sequences (240000216 bp)... [M::process] 0 single-end sequences; 1925938 paired-end sequences [M::process] read 1926010 sequences (240000208 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1277, 752240, 60, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 107, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (123.62, 72.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 168, 2343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6953) [M::mem_pestat] mean and std.dev: (1079.50, 1592.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9258) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (170, 270, 1032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2756) [M::mem_pestat] mean and std.dev: (525.76, 578.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3618) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925938 reads in 997.609 CPU sec, 63.679 real sec [M::process] 0 single-end sequences; 1926010 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1270, 751892, 68, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 119, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (130.80, 75.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.33, 30.22) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 172, 2673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7949) [M::mem_pestat] mean and std.dev: (1008.47, 1613.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10587) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (113, 183, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 917) [M::mem_pestat] mean and std.dev: (196.64, 158.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1185) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1926010 reads in 965.711 CPU sec, 42.599 real sec [M::process] read 1925814 sequences (240000112 bp)... [M::process] 0 single-end sequences; 1925814 paired-end sequences [M::process] read 1925962 sequences (240000186 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1211, 751563, 44, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 111, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (127.69, 73.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.10) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 163, 1960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5830) [M::mem_pestat] mean and std.dev: (738.30, 1336.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7765) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (109, 188, 880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2422) [M::mem_pestat] mean and std.dev: (439.79, 566.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3193) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925814 reads in 1005.834 CPU sec, 63.926 real sec [M::process] 0 single-end sequences; 1925962 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1280, 752552, 53, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 108, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (124.30, 73.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 69, 919) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2721) [M::mem_pestat] mean and std.dev: (189.57, 301.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3622) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (84, 196, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1776) [M::mem_pestat] mean and std.dev: (324.28, 359.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2340) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925962 reads in 959.769 CPU sec, 42.279 real sec [M::process] read 1925850 sequences (240000234 bp)... [M::process] 0 single-end sequences; 1925850 paired-end sequences [M::process] read 1925910 sequences (240000002 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1302, 752210, 51, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 111, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (126.32, 74.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.46, 30.08) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (61, 187, 3519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10435) [M::mem_pestat] mean and std.dev: (1879.59, 2587.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13893) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (122, 193, 1271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3569) [M::mem_pestat] mean and std.dev: (537.87, 790.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4718) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925850 reads in 994.937 CPU sec, 64.815 real sec [M::process] 0 single-end sequences; 1925910 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1293, 751535, 44, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 113, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (124.20, 71.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.11) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 312, 2985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8905) [M::mem_pestat] mean and std.dev: (1622.34, 1996.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11865) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (98, 183, 647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1745) [M::mem_pestat] mean and std.dev: (337.92, 425.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2294) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925910 reads in 960.660 CPU sec, 42.235 real sec [M::process] read 1925958 sequences (240000152 bp)... [M::process] 0 single-end sequences; 1925958 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1280, 751694, 44, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 114, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (127.59, 74.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.31, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (86, 939, 3073) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9047) [M::mem_pestat] mean and std.dev: (1561.67, 1690.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12034) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 241, 1141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3135) [M::mem_pestat] mean and std.dev: (578.47, 703.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4132) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925958 reads in 987.577 CPU sec, 55.674 real sec [M::process] read 1925914 sequences (240000184 bp)... [M::process] 0 single-end sequences; 1925914 paired-end sequences [M::process] read 1925918 sequences (240000096 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1210, 752565, 45, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 106, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (121.84, 72.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 167, 2047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6069) [M::mem_pestat] mean and std.dev: (947.26, 1493.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8080) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 201, 895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2419) [M::mem_pestat] mean and std.dev: (483.03, 574.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3181) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925914 reads in 985.961 CPU sec, 62.324 real sec [M::process] 0 single-end sequences; 1925918 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1316, 751441, 36, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 109, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (123.23, 72.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (55, 288, 4377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13021) [M::mem_pestat] mean and std.dev: (1761.67, 2610.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17343) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (120, 178, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (185.82, 139.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 956) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925918 reads in 972.152 CPU sec, 43.991 real sec [M::process] read 1925998 sequences (240000170 bp)... [M::process] 0 single-end sequences; 1925998 paired-end sequences [M::process] read 1925918 sequences (240000144 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1247, 752415, 49, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 109, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (126.00, 73.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 38, 580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1704) [M::mem_pestat] mean and std.dev: (122.95, 252.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2266) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (175, 249, 926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2428) [M::mem_pestat] mean and std.dev: (481.53, 513.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3179) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925998 reads in 998.311 CPU sec, 63.518 real sec [M::process] 0 single-end sequences; 1925918 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1206, 751781, 52, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 110, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (127.05, 75.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 429, 2021) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5997) [M::mem_pestat] mean and std.dev: (1300.43, 1689.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8060) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (164, 265, 1119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3029) [M::mem_pestat] mean and std.dev: (515.38, 571.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3984) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925918 reads in 965.914 CPU sec, 42.646 real sec [M::process] read 1925908 sequences (240000094 bp)... [M::process] 0 single-end sequences; 1925908 paired-end sequences [M::process] read 1926004 sequences (240000036 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1322, 751720, 46, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 104, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (120.97, 72.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.32, 30.21) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 85, 2410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7190) [M::mem_pestat] mean and std.dev: (1208.89, 1692.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9580) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (132, 209, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963) [M::mem_pestat] mean and std.dev: (229.05, 178.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1240) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925908 reads in 997.401 CPU sec, 64.094 real sec [M::process] 0 single-end sequences; 1926004 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1246, 752302, 42, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 112, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (126.86, 73.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.21) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 133, 2738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8140) [M::mem_pestat] mean and std.dev: (1299.37, 1824.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10841) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (112, 230, 1084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3028) [M::mem_pestat] mean and std.dev: (484.31, 665.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4000) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1926004 reads in 955.739 CPU sec, 42.099 real sec [M::process] read 1925892 sequences (240000100 bp)... [M::process] 0 single-end sequences; 1925892 paired-end sequences [M::process] read 1925932 sequences (240000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1279, 752174, 46, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 111, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (124.36, 72.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.43, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 369, 2602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7752) [M::mem_pestat] mean and std.dev: (1291.69, 1576.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10327) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (94, 180, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 967) [M::mem_pestat] mean and std.dev: (221.38, 203.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1258) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925892 reads in 1006.277 CPU sec, 65.547 real sec [M::process] 0 single-end sequences; 1925932 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1277, 752049, 47, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 108, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (124.19, 73.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (379, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (295, 505) [M::mem_pestat] mean and std.dev: (403.69, 29.69) [M::mem_pestat] low and high boundaries for proper pairs: (253, 547) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 69, 1290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3828) [M::mem_pestat] mean and std.dev: (275.79, 656.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5097) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (127, 214, 765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2041) [M::mem_pestat] mean and std.dev: (416.12, 492.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2679) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925932 reads in 947.822 CPU sec, 41.666 real sec [M::process] read 1925944 sequences (240000066 bp)... [M::process] 0 single-end sequences; 1925944 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1240, 752552, 52, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 112, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (125.21, 71.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.43, 30.09) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 128, 2468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7358) [M::mem_pestat] mean and std.dev: (1236.21, 1814.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9803) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (127, 182, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 604) [M::mem_pestat] mean and std.dev: (162.00, 104.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925944 reads in 1000.393 CPU sec, 66.459 real sec [M::process] read 1925940 sequences (240000180 bp)... [M::process] 0 single-end sequences; 1925940 paired-end sequences [M::process] read 1925892 sequences (240000158 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1239, 751721, 51, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 113, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (125.06, 72.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 136, 3046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9070) [M::mem_pestat] mean and std.dev: (1591.42, 1931.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12082) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 248, 1125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3117) [M::mem_pestat] mean and std.dev: (567.90, 727.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4113) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925940 reads in 997.339 CPU sec, 61.901 real sec [M::process] 0 single-end sequences; 1925892 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1202, 752892, 44, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 104, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (121.46, 72.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.10) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 176, 2664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7942) [M::mem_pestat] mean and std.dev: (1188.61, 1717.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10581) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (102, 188, 1089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3063) [M::mem_pestat] mean and std.dev: (409.23, 542.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4050) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925892 reads in 971.322 CPU sec, 43.948 real sec [M::process] read 1925892 sequences (240000024 bp)... [M::process] 0 single-end sequences; 1925892 paired-end sequences [M::process] read 1925866 sequences (240000100 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1201, 751643, 58, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 114, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (125.36, 72.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.08) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 143, 1753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5185) [M::mem_pestat] mean and std.dev: (1001.98, 1510.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7044) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 305, 1098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2992) [M::mem_pestat] mean and std.dev: (688.73, 711.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3939) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925892 reads in 988.507 CPU sec, 63.140 real sec [M::process] 0 single-end sequences; 1925866 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1277, 751526, 52, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 109, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (123.12, 73.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 720, 2613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7765) [M::mem_pestat] mean and std.dev: (1500.52, 1862.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10341) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (75, 174, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1032) [M::mem_pestat] mean and std.dev: (202.29, 214.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1351) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925866 reads in 948.177 CPU sec, 41.925 real sec [M::process] read 1925892 sequences (240000216 bp)... [M::process] 0 single-end sequences; 1925892 paired-end sequences [M::process] read 1925930 sequences (240000202 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1277, 751499, 37, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 114, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (127.59, 73.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.10) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 139, 2677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7995) [M::mem_pestat] mean and std.dev: (1234.65, 2049.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10654) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (97, 175, 788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2170) [M::mem_pestat] mean and std.dev: (347.40, 419.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2861) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925892 reads in 1005.033 CPU sec, 64.388 real sec [M::process] 0 single-end sequences; 1925930 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1288, 752251, 37, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 112, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (124.53, 72.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 90, 1983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5899) [M::mem_pestat] mean and std.dev: (698.48, 1359.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7857) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 237, 833) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2187) [M::mem_pestat] mean and std.dev: (356.50, 381.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2864) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925930 reads in 961.822 CPU sec, 42.432 real sec [M::process] read 1925898 sequences (240000120 bp)... [M::process] 0 single-end sequences; 1925898 paired-end sequences [M::process] read 1925826 sequences (240000070 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1240, 752416, 41, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 117, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (127.35, 73.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.28, 30.22) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 166, 2612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7788) [M::mem_pestat] mean and std.dev: (1195.40, 1736.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10376) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (84, 187, 358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 906) [M::mem_pestat] mean and std.dev: (182.62, 170.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1180) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925898 reads in 989.071 CPU sec, 64.250 real sec [M::process] 0 single-end sequences; 1925826 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1291, 751507, 41, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 109, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (123.58, 73.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 160, 2615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7813) [M::mem_pestat] mean and std.dev: (1215.95, 1743.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10412) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (116, 192, 1221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3431) [M::mem_pestat] mean and std.dev: (503.80, 610.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4536) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925826 reads in 963.464 CPU sec, 42.888 real sec [M::process] read 1925988 sequences (240000170 bp)... [M::process] 0 single-end sequences; 1925988 paired-end sequences [M::process] read 1925970 sequences (240000002 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1222, 751671, 63, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 109, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (124.40, 72.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (63, 205, 3025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8949) [M::mem_pestat] mean and std.dev: (1526.16, 2062.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11911) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (105, 271, 1331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3783) [M::mem_pestat] mean and std.dev: (751.73, 905.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5009) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925988 reads in 985.998 CPU sec, 65.110 real sec [M::process] 0 single-end sequences; 1925970 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1287, 751947, 52, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 114, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (126.70, 73.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 116, 3062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9150) [M::mem_pestat] mean and std.dev: (1581.38, 2540.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12194) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (228, 680, 1567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4245) [M::mem_pestat] mean and std.dev: (860.91, 973.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5584) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925970 reads in 957.496 CPU sec, 42.139 real sec [M::process] read 1925870 sequences (240000212 bp)... [M::process] 0 single-end sequences; 1925870 paired-end sequences [M::process] read 1925902 sequences (240000212 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1247, 751683, 58, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 111, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (126.76, 74.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 52, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1237) [M::mem_pestat] mean and std.dev: (68.30, 83.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1645) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (106, 179, 1054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2950) [M::mem_pestat] mean and std.dev: (442.91, 592.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3898) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925870 reads in 1002.738 CPU sec, 63.190 real sec [M::process] 0 single-end sequences; 1925902 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1325, 752162, 50, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 108, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (124.15, 72.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.22) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 140, 1139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3379) [M::mem_pestat] mean and std.dev: (479.11, 831.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4499) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (109, 184, 909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2509) [M::mem_pestat] mean and std.dev: (520.94, 673.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3309) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925902 reads in 968.115 CPU sec, 43.907 real sec [M::process] read 1925898 sequences (240000218 bp)... [M::process] 0 single-end sequences; 1925898 paired-end sequences [M::process] read 1925962 sequences (240000068 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1244, 751802, 47, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 112, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (124.68, 71.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 175, 2863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8495) [M::mem_pestat] mean and std.dev: (1212.38, 1710.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11311) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (104, 188, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 590) [M::mem_pestat] mean and std.dev: (178.56, 126.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925898 reads in 999.658 CPU sec, 63.142 real sec [M::process] 0 single-end sequences; 1925962 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1197, 752211, 49, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 106, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (123.10, 73.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.44, 30.04) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 144, 2830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8420) [M::mem_pestat] mean and std.dev: (1086.17, 1644.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11215) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (66, 182, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (145.38, 99.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 782) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925962 reads in 966.775 CPU sec, 42.723 real sec [M::process] read 1925950 sequences (240000150 bp)... [M::process] 0 single-end sequences; 1925950 paired-end sequences [M::process] read 1925926 sequences (240000092 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1231, 752260, 52, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 115, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (126.36, 72.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.29, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 137, 1687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5011) [M::mem_pestat] mean and std.dev: (924.37, 1569.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7202) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (80, 178, 432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1136) [M::mem_pestat] mean and std.dev: (254.74, 283.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1488) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925950 reads in 999.722 CPU sec, 65.175 real sec [M::process] 0 single-end sequences; 1925926 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1264, 751482, 47, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 114, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (126.13, 71.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 119, 788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2314) [M::mem_pestat] mean and std.dev: (247.95, 421.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3077) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (121, 203, 1037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2869) [M::mem_pestat] mean and std.dev: (454.19, 540.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3785) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925926 reads in 954.010 CPU sec, 41.971 real sec [M::process] read 1925908 sequences (240000016 bp)... [M::process] 0 single-end sequences; 1925908 paired-end sequences [M::process] read 1925788 sequences (240000134 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1316, 751657, 48, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 109, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (125.20, 74.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 174, 1956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5818) [M::mem_pestat] mean and std.dev: (1074.38, 1572.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7749) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (172, 280, 1243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3385) [M::mem_pestat] mean and std.dev: (592.19, 651.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4456) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925908 reads in 998.454 CPU sec, 63.748 real sec [M::process] 0 single-end sequences; 1925788 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1249, 751637, 34, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 114, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (127.73, 74.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (59, 210, 3658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10856) [M::mem_pestat] mean and std.dev: (2028.91, 2820.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14455) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (121, 221, 1015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2803) [M::mem_pestat] mean and std.dev: (522.27, 573.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3697) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925788 reads in 954.601 CPU sec, 41.991 real sec [M::process] read 1925852 sequences (240000236 bp)... [M::process] 0 single-end sequences; 1925852 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1224, 752633, 46, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 112, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (125.68, 73.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.42, 30.06) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 93, 663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1915) [M::mem_pestat] mean and std.dev: (237.97, 417.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2541) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (94, 189, 581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1555) [M::mem_pestat] mean and std.dev: (346.72, 386.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2042) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925852 reads in 995.102 CPU sec, 62.824 real sec [M::process] read 1925816 sequences (240000030 bp)... [M::process] 0 single-end sequences; 1925816 paired-end sequences [M::process] read 1925842 sequences (240000112 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1241, 752401, 48, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 106, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (124.78, 74.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 103, 4386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13118) [M::mem_pestat] mean and std.dev: (1714.08, 2499.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17484) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (97, 213, 1277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3637) [M::mem_pestat] mean and std.dev: (549.48, 679.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4817) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925816 reads in 995.298 CPU sec, 63.155 real sec [M::process] 0 single-end sequences; 1925842 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1285, 751206, 45, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 110, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (125.13, 71.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 93, 2948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8820) [M::mem_pestat] mean and std.dev: (1278.56, 1851.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11756) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 214, 839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2251) [M::mem_pestat] mean and std.dev: (459.52, 554.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2957) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925842 reads in 969.312 CPU sec, 43.153 real sec [M::process] read 1925962 sequences (240000162 bp)... [M::process] 0 single-end sequences; 1925962 paired-end sequences [M::process] read 1925914 sequences (240000190 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1235, 752292, 50, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 109, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (121.99, 72.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.45, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 94, 569) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1669) [M::mem_pestat] mean and std.dev: (82.11, 108.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2219) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (95, 159, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (169.10, 126.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925962 reads in 992.868 CPU sec, 63.651 real sec [M::process] 0 single-end sequences; 1925914 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1267, 751674, 53, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 112, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (124.09, 71.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.43, 30.10) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 140, 4375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13067) [M::mem_pestat] mean and std.dev: (1553.38, 2188.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17413) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (68, 204, 1103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3173) [M::mem_pestat] mean and std.dev: (547.33, 725.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4208) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925914 reads in 960.859 CPU sec, 42.558 real sec [M::process] read 1925874 sequences (240000242 bp)... [M::process] 0 single-end sequences; 1925874 paired-end sequences [M::process] read 1925840 sequences (240000216 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1203, 752112, 49, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 109, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (122.88, 73.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 90, 3037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9077) [M::mem_pestat] mean and std.dev: (1295.55, 1933.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12097) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (118, 253, 1191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3337) [M::mem_pestat] mean and std.dev: (713.75, 896.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4410) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925874 reads in 989.339 CPU sec, 62.866 real sec [M::process] 0 single-end sequences; 1925840 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1267, 751467, 61, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 111, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (124.39, 73.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.33, 30.22) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 55, 3028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9038) [M::mem_pestat] mean and std.dev: (1492.12, 2300.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12043) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (112, 202, 1179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3313) [M::mem_pestat] mean and std.dev: (585.39, 713.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4380) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925840 reads in 952.772 CPU sec, 41.921 real sec [M::process] read 1925870 sequences (240000160 bp)... [M::process] 0 single-end sequences; 1925870 paired-end sequences [M::process] read 1925990 sequences (240000094 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1210, 752039, 52, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 113, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (126.00, 73.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 133, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3415) [M::mem_pestat] mean and std.dev: (268.48, 471.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4544) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (89, 183, 742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2048) [M::mem_pestat] mean and std.dev: (380.71, 492.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2701) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925870 reads in 997.505 CPU sec, 64.669 real sec [M::process] 0 single-end sequences; 1925990 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1239, 752113, 54, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 106, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (123.16, 75.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.42, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 101, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1059) [M::mem_pestat] mean and std.dev: (97.88, 106.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1405) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (117, 202, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (216.67, 185.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1221) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925990 reads in 959.514 CPU sec, 42.172 real sec [M::process] read 1925950 sequences (240000046 bp)... [M::process] 0 single-end sequences; 1925950 paired-end sequences [M::process] read 1925888 sequences (240000008 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1199, 751871, 46, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 110, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (124.12, 73.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 78, 1621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4831) [M::mem_pestat] mean and std.dev: (1038.83, 1559.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7277) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 194, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1294) [M::mem_pestat] mean and std.dev: (282.65, 278.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1679) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925950 reads in 981.178 CPU sec, 65.028 real sec [M::process] 0 single-end sequences; 1925888 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1277, 751993, 52, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 111, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (123.37, 71.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.20) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 138, 3258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9722) [M::mem_pestat] mean and std.dev: (1657.54, 2448.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12954) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 208, 846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2224) [M::mem_pestat] mean and std.dev: (348.17, 398.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2913) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925888 reads in 963.278 CPU sec, 42.401 real sec [M::process] read 1925924 sequences (240000074 bp)... [M::process] 0 single-end sequences; 1925924 paired-end sequences [M::process] read 1925954 sequences (240000090 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1309, 750949, 44, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 107, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (124.27, 73.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.33, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 115, 2758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8226) [M::mem_pestat] mean and std.dev: (1203.40, 1947.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10960) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (147, 193, 941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2529) [M::mem_pestat] mean and std.dev: (533.02, 675.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3323) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925924 reads in 985.893 CPU sec, 63.030 real sec [M::process] 0 single-end sequences; 1925954 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1223, 751691, 47, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 112, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (127.66, 74.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 484, 4403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13131) [M::mem_pestat] mean and std.dev: (1905.98, 2536.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17495) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (93, 157, 604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1626) [M::mem_pestat] mean and std.dev: (321.68, 398.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2137) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925954 reads in 960.878 CPU sec, 43.538 real sec [M::process] read 1925838 sequences (240000100 bp)... [M::process] 0 single-end sequences; 1925838 paired-end sequences [M::process] read 1925954 sequences (240000240 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1234, 752495, 48, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 109, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (125.47, 73.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 600, 4378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13086) [M::mem_pestat] mean and std.dev: (1960.92, 2266.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17440) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (62, 194, 1137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3287) [M::mem_pestat] mean and std.dev: (448.85, 532.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4362) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925838 reads in 1000.654 CPU sec, 64.119 real sec [M::process] 0 single-end sequences; 1925954 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1261, 752346, 40, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 108, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (122.86, 72.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 148, 1284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3798) [M::mem_pestat] mean and std.dev: (515.00, 972.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5055) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 192, 579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1431) [M::mem_pestat] mean and std.dev: (289.53, 315.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1857) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925954 reads in 968.304 CPU sec, 42.735 real sec [M::process] read 1925888 sequences (240000086 bp)... [M::process] 0 single-end sequences; 1925888 paired-end sequences [M::process] read 1925922 sequences (240000148 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1254, 752504, 52, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 114, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (126.89, 73.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.20) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 175, 2640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7870) [M::mem_pestat] mean and std.dev: (1233.55, 1838.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10485) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (125, 232, 811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2183) [M::mem_pestat] mean and std.dev: (382.71, 422.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2869) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925888 reads in 998.762 CPU sec, 63.117 real sec [M::process] 0 single-end sequences; 1925922 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1228, 752086, 54, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 107, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (123.14, 72.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 163, 4378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13092) [M::mem_pestat] mean and std.dev: (1808.22, 2410.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17449) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (111, 191, 997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2769) [M::mem_pestat] mean and std.dev: (485.39, 640.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3655) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925922 reads in 958.338 CPU sec, 42.163 real sec [M::process] read 1926006 sequences (240000062 bp)... [M::process] 0 single-end sequences; 1926006 paired-end sequences [M::process] read 1925958 sequences (240000152 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1261, 751812, 58, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 116, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (128.87, 73.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 184, 2675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7981) [M::mem_pestat] mean and std.dev: (1324.74, 1799.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10634) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 211, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 817) [M::mem_pestat] mean and std.dev: (205.40, 138.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1037) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1926006 reads in 981.158 CPU sec, 62.479 real sec [M::process] 0 single-end sequences; 1925958 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1291, 752034, 50, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 111, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (124.25, 72.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.42, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 113, 1960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5840) [M::mem_pestat] mean and std.dev: (1016.98, 1553.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7780) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (119, 249, 1008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2786) [M::mem_pestat] mean and std.dev: (539.47, 645.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3675) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925958 reads in 955.005 CPU sec, 43.481 real sec [M::process] read 1925854 sequences (240000104 bp)... [M::process] 0 single-end sequences; 1925854 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1300, 751629, 58, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 113, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (125.98, 74.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 148, 2711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8065) [M::mem_pestat] mean and std.dev: (1258.54, 1667.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10742) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 240, 1416) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3926) [M::mem_pestat] mean and std.dev: (715.17, 896.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5181) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925854 reads in 981.555 CPU sec, 58.247 real sec [M::process] read 1925888 sequences (240000198 bp)... [M::process] 0 single-end sequences; 1925888 paired-end sequences [M::process] read 1925844 sequences (240000188 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1232, 751893, 39, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 110, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (125.33, 73.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.21) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 70, 1767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5263) [M::mem_pestat] mean and std.dev: (970.10, 1589.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7327) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (98, 252, 1281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3647) [M::mem_pestat] mean and std.dev: (633.77, 863.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4830) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925888 reads in 999.681 CPU sec, 62.014 real sec [M::process] 0 single-end sequences; 1925844 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1232, 751934, 54, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 109, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (124.06, 71.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 114, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1440) [M::mem_pestat] mean and std.dev: (111.69, 162.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1912) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (106, 176, 1191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3361) [M::mem_pestat] mean and std.dev: (492.00, 652.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4446) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925844 reads in 951.290 CPU sec, 43.549 real sec [M::process] read 1925952 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1925952 paired-end sequences [M::process] read 1925882 sequences (240000176 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1300, 750992, 47, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 110, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (125.00, 72.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 135, 3076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9164) [M::mem_pestat] mean and std.dev: (1628.13, 2431.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12208) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (95, 190, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (165.21, 124.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925952 reads in 1000.710 CPU sec, 64.137 real sec [M::process] 0 single-end sequences; 1925882 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1195, 752205, 56, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 106, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (120.88, 71.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.45, 30.10) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 132, 2497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7447) [M::mem_pestat] mean and std.dev: (1186.80, 1692.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9922) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (83, 167, 1020) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2894) [M::mem_pestat] mean and std.dev: (459.44, 621.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3831) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925882 reads in 959.113 CPU sec, 42.313 real sec [M::process] read 1925874 sequences (240000216 bp)... [M::process] 0 single-end sequences; 1925874 paired-end sequences [M::process] read 1926002 sequences (240000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1304, 751563, 44, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 113, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (123.75, 72.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.31, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 474, 2621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7813) [M::mem_pestat] mean and std.dev: (1235.71, 1554.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10409) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (118, 204, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (163.26, 94.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925874 reads in 1006.669 CPU sec, 63.925 real sec [M::process] 0 single-end sequences; 1926002 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1195, 752068, 41, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 110, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (124.47, 71.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 193, 2684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8016) [M::mem_pestat] mean and std.dev: (1089.84, 1462.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10682) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (127, 209, 1119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3103) [M::mem_pestat] mean and std.dev: (597.59, 717.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4095) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1926002 reads in 956.621 CPU sec, 42.235 real sec [M::process] read 1925904 sequences (240000032 bp)... [M::process] 0 single-end sequences; 1925904 paired-end sequences [M::process] read 1925876 sequences (240000140 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1252, 751952, 56, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 107, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (123.37, 73.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 168, 2048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6090) [M::mem_pestat] mean and std.dev: (982.15, 1516.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8111) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 803, 1531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4289) [M::mem_pestat] mean and std.dev: (821.36, 800.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5668) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925904 reads in 998.173 CPU sec, 63.893 real sec [M::process] 0 single-end sequences; 1925876 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1241, 752334, 43, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 106, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (122.67, 72.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.11) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 166, 4096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12222) [M::mem_pestat] mean and std.dev: (1690.81, 2291.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16285) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (105, 179, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (140.77, 73.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925876 reads in 952.219 CPU sec, 41.968 real sec [M::process] read 1925944 sequences (240000008 bp)... [M::process] 0 single-end sequences; 1925944 paired-end sequences [M::process] read 1925898 sequences (240000046 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1301, 752485, 51, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 111, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (124.72, 73.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.11) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 65, 856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2544) [M::mem_pestat] mean and std.dev: (158.51, 310.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3388) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (121, 234, 946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2596) [M::mem_pestat] mean and std.dev: (477.83, 603.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3421) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925944 reads in 997.800 CPU sec, 64.430 real sec [M::process] 0 single-end sequences; 1925898 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1214, 751737, 50, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 112, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (125.35, 72.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.30, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 139, 2032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6016) [M::mem_pestat] mean and std.dev: (1074.50, 1736.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8019) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (107, 240, 1000) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2786) [M::mem_pestat] mean and std.dev: (546.05, 646.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3679) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925898 reads in 952.955 CPU sec, 41.874 real sec [M::process] read 1925974 sequences (240000228 bp)... [M::process] 0 single-end sequences; 1925974 paired-end sequences [M::process] read 1925894 sequences (240000112 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1253, 751986, 47, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 117, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (130.00, 75.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 127, 2627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7845) [M::mem_pestat] mean and std.dev: (1107.67, 1719.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10454) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (94, 196, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2194) [M::mem_pestat] mean and std.dev: (430.33, 517.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2894) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925974 reads in 999.884 CPU sec, 62.347 real sec [M::process] 0 single-end sequences; 1925894 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1272, 751539, 45, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 111, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (124.94, 73.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 126, 2849) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8497) [M::mem_pestat] mean and std.dev: (1294.36, 1797.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11321) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 191, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1256) [M::mem_pestat] mean and std.dev: (239.44, 237.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1629) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925894 reads in 967.271 CPU sec, 44.316 real sec [M::process] read 1925886 sequences (240000036 bp)... [M::process] 0 single-end sequences; 1925886 paired-end sequences [M::process] read 1925948 sequences (240000174 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1262, 752201, 47, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 108, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (124.42, 73.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.32, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 77, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 690) [M::mem_pestat] mean and std.dev: (78.05, 80.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 912) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 205, 994) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2720) [M::mem_pestat] mean and std.dev: (506.21, 628.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3583) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925886 reads in 991.101 CPU sec, 62.711 real sec [M::process] 0 single-end sequences; 1925948 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1222, 752143, 44, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 112, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (123.56, 69.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 82, 1553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4599) [M::mem_pestat] mean and std.dev: (1009.50, 1628.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7523) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (74, 222, 934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2654) [M::mem_pestat] mean and std.dev: (454.88, 569.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3514) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925948 reads in 955.403 CPU sec, 43.246 real sec [M::process] read 1925956 sequences (240000208 bp)... [M::process] 0 single-end sequences; 1925956 paired-end sequences [M::process] read 1925846 sequences (240000172 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1218, 752424, 56, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 110, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (123.37, 72.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 162, 2814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8390) [M::mem_pestat] mean and std.dev: (1268.04, 1704.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11178) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 194, 1172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3202) [M::mem_pestat] mean and std.dev: (456.27, 504.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4217) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925956 reads in 993.112 CPU sec, 62.905 real sec [M::process] 0 single-end sequences; 1925846 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1245, 751924, 46, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 116, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (126.65, 72.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.21) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (53, 314, 2564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7586) [M::mem_pestat] mean and std.dev: (1230.16, 1725.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10097) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (86, 181, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 956) [M::mem_pestat] mean and std.dev: (203.63, 186.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1246) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925846 reads in 961.397 CPU sec, 42.457 real sec [M::process] read 1925948 sequences (240000074 bp)... [M::process] 0 single-end sequences; 1925948 paired-end sequences [M::process] read 1925864 sequences (240000052 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1230, 751840, 41, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 109, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (123.41, 72.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 115, 1037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3065) [M::mem_pestat] mean and std.dev: (507.21, 847.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4079) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (96, 178, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1527) [M::mem_pestat] mean and std.dev: (338.86, 400.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2004) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925948 reads in 990.884 CPU sec, 64.007 real sec [M::process] 0 single-end sequences; 1925864 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1248, 752229, 52, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 115, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (125.67, 73.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 403, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.45, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 133, 2668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7934) [M::mem_pestat] mean and std.dev: (1117.46, 1682.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10567) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (110, 227, 1170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3290) [M::mem_pestat] mean and std.dev: (684.00, 862.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4350) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925864 reads in 959.671 CPU sec, 42.239 real sec [M::process] read 1925944 sequences (240000088 bp)... [M::process] 0 single-end sequences; 1925944 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1228, 752416, 41, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 107, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (122.93, 72.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.21) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 125, 3848) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11500) [M::mem_pestat] mean and std.dev: (1871.61, 2703.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15326) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (84, 165, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (135.54, 102.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925944 reads in 963.165 CPU sec, 54.887 real sec [M::process] read 1925884 sequences (240000128 bp)... [M::process] 0 single-end sequences; 1925884 paired-end sequences [M::process] read 1925922 sequences (240000026 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1216, 751777, 41, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 110, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (125.28, 71.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 121, 3041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9079) [M::mem_pestat] mean and std.dev: (1498.93, 2539.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12098) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (101, 193, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (170.95, 100.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 841) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925884 reads in 1008.013 CPU sec, 62.691 real sec [M::process] 0 single-end sequences; 1925922 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1222, 752131, 41, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 111, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (123.66, 71.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.43, 30.11) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 154, 4380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13090) [M::mem_pestat] mean and std.dev: (1788.63, 2661.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17445) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (82, 187, 574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1558) [M::mem_pestat] mean and std.dev: (313.33, 414.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2050) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925922 reads in 969.026 CPU sec, 44.226 real sec [M::process] read 1925932 sequences (240000176 bp)... [M::process] 0 single-end sequences; 1925932 paired-end sequences [M::process] read 1925894 sequences (240000186 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1242, 751985, 48, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 116, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (129.04, 74.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.32, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 152, 2023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5999) [M::mem_pestat] mean and std.dev: (1191.72, 1742.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8161) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (80, 160, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (147.48, 112.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 872) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925932 reads in 1000.217 CPU sec, 63.511 real sec [M::process] 0 single-end sequences; 1925894 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1204, 752114, 39, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 106, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (122.74, 73.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.23) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 46, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1627) [M::mem_pestat] mean and std.dev: (189.48, 322.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2164) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 216, 1140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3146) [M::mem_pestat] mean and std.dev: (532.79, 626.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4149) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925894 reads in 957.438 CPU sec, 42.257 real sec [M::process] read 1925838 sequences (240000248 bp)... [M::process] 0 single-end sequences; 1925838 paired-end sequences [M::process] read 1925966 sequences (240000134 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1299, 752320, 57, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 115, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (128.08, 73.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 249, 4070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12110) [M::mem_pestat] mean and std.dev: (1687.26, 2112.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16130) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 251, 910) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2422) [M::mem_pestat] mean and std.dev: (450.81, 512.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3178) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925838 reads in 997.117 CPU sec, 63.343 real sec [M::process] 0 single-end sequences; 1925966 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1246, 751991, 45, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 113, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (126.76, 71.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.43, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 595, 4393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13093) [M::mem_pestat] mean and std.dev: (1924.67, 2285.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17443) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (107, 178, 584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1538) [M::mem_pestat] mean and std.dev: (294.98, 373.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2015) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925966 reads in 958.451 CPU sec, 42.486 real sec [M::process] read 1926010 sequences (240000164 bp)... [M::process] 0 single-end sequences; 1926010 paired-end sequences [M::process] read 1925826 sequences (240000078 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1283, 751964, 47, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 110, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (123.87, 71.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.44, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (93, 182, 2371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6927) [M::mem_pestat] mean and std.dev: (1152.17, 1587.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9205) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (100, 196, 1292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3676) [M::mem_pestat] mean and std.dev: (580.47, 749.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4868) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1926010 reads in 976.824 CPU sec, 64.948 real sec [M::process] 0 single-end sequences; 1925826 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1220, 751584, 47, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 113, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (126.88, 72.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 167, 2221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6617) [M::mem_pestat] mean and std.dev: (873.43, 1409.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8815) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (194, 263, 1253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3371) [M::mem_pestat] mean and std.dev: (605.86, 636.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4430) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925826 reads in 957.624 CPU sec, 42.130 real sec [M::process] read 1925894 sequences (240000124 bp)... [M::process] 0 single-end sequences; 1925894 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1226, 752058, 53, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 110, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (123.73, 71.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 91, 3032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9060) [M::mem_pestat] mean and std.dev: (1390.70, 1937.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12074) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (110, 215, 786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2138) [M::mem_pestat] mean and std.dev: (504.74, 625.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3006) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925894 reads in 1012.708 CPU sec, 65.828 real sec [M::process] read 1925764 sequences (240000246 bp)... [M::process] 0 single-end sequences; 1925764 paired-end sequences [M::process] read 1925950 sequences (240000072 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1205, 751285, 42, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 117, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (128.57, 73.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 456, 4388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13116) [M::mem_pestat] mean and std.dev: (2318.60, 3063.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17480) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (102, 256, 1013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2835) [M::mem_pestat] mean and std.dev: (599.00, 678.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3746) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925764 reads in 999.557 CPU sec, 62.123 real sec [M::process] 0 single-end sequences; 1925950 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1211, 751619, 54, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 112, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (124.34, 72.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 139, 1988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5892) [M::mem_pestat] mean and std.dev: (1017.33, 1624.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7844) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (125, 219, 921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2513) [M::mem_pestat] mean and std.dev: (454.79, 519.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3309) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925950 reads in 965.550 CPU sec, 43.923 real sec [M::process] read 1925950 sequences (240000032 bp)... [M::process] 0 single-end sequences; 1925950 paired-end sequences [M::process] read 1925918 sequences (240000058 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1265, 751833, 54, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 116, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (127.16, 70.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.06) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 120, 2175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6489) [M::mem_pestat] mean and std.dev: (976.12, 1618.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8646) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 259, 1273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3521) [M::mem_pestat] mean and std.dev: (706.88, 809.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4645) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925950 reads in 998.199 CPU sec, 64.949 real sec [M::process] 0 single-end sequences; 1925918 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1265, 752622, 51, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 111, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (125.84, 75.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.29, 30.20) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 141, 1489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4421) [M::mem_pestat] mean and std.dev: (560.00, 1091.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5887) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (99, 185, 890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2472) [M::mem_pestat] mean and std.dev: (462.59, 625.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3263) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925918 reads in 950.904 CPU sec, 41.956 real sec [M::process] read 1925912 sequences (240000040 bp)... [M::process] 0 single-end sequences; 1925912 paired-end sequences [M::process] read 1925872 sequences (240000010 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1295, 752003, 58, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 106, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (121.88, 71.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.46, 30.10) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 134, 2666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7942) [M::mem_pestat] mean and std.dev: (1219.95, 1771.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10580) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (76, 175, 896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2536) [M::mem_pestat] mean and std.dev: (393.67, 558.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3356) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925912 reads in 1008.961 CPU sec, 63.327 real sec [M::process] 0 single-end sequences; 1925872 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1185, 752324, 54, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 115, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (129.87, 73.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.44, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 145, 2259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6735) [M::mem_pestat] mean and std.dev: (1161.49, 1753.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8973) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (91, 204, 754) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2080) [M::mem_pestat] mean and std.dev: (343.00, 419.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2743) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925872 reads in 966.572 CPU sec, 44.140 real sec [M::process] read 1926002 sequences (240000120 bp)... [M::process] 0 single-end sequences; 1926002 paired-end sequences [M::process] read 1925922 sequences (240000102 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1258, 751459, 60, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 112, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (125.35, 72.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 75, 1491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4429) [M::mem_pestat] mean and std.dev: (817.07, 1455.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6640) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (102, 213, 1415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4041) [M::mem_pestat] mean and std.dev: (454.52, 663.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5354) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1926002 reads in 1003.995 CPU sec, 63.973 real sec [M::process] 0 single-end sequences; 1925922 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1207, 751675, 43, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 114, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (127.84, 73.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 129, 2492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7444) [M::mem_pestat] mean and std.dev: (1229.02, 1858.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9920) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 197, 884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2370) [M::mem_pestat] mean and std.dev: (476.75, 548.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3113) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925922 reads in 954.727 CPU sec, 42.200 real sec [M::process] read 1925882 sequences (240000038 bp)... [M::process] 0 single-end sequences; 1925882 paired-end sequences [M::process] read 1925878 sequences (240000148 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1270, 751879, 47, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 109, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (124.98, 73.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 66, 1407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4181) [M::mem_pestat] mean and std.dev: (450.12, 868.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5568) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (82, 187, 1129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3223) [M::mem_pestat] mean and std.dev: (496.16, 630.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4270) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925882 reads in 999.476 CPU sec, 63.741 real sec [M::process] 0 single-end sequences; 1925878 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1236, 752422, 51, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 111, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (124.27, 73.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.31, 30.21) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 306, 2714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8080) [M::mem_pestat] mean and std.dev: (1412.00, 1750.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10763) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (96, 193, 880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2448) [M::mem_pestat] mean and std.dev: (457.00, 562.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3232) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925878 reads in 968.602 CPU sec, 42.598 real sec [M::process] read 1925838 sequences (240000118 bp)... [M::process] 0 single-end sequences; 1925838 paired-end sequences [M::process] read 1925876 sequences (240000192 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1176, 752438, 54, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 108, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (123.55, 71.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.10) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 61, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (79.09, 93.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (107, 188, 830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2276) [M::mem_pestat] mean and std.dev: (263.03, 289.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2999) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925838 reads in 992.131 CPU sec, 61.650 real sec [M::process] 0 single-end sequences; 1925876 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1251, 751466, 53, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 108, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (123.55, 73.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 89, 2949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8791) [M::mem_pestat] mean and std.dev: (1366.09, 2279.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11712) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (115, 197, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (163.32, 84.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925876 reads in 972.738 CPU sec, 44.520 real sec [M::process] read 1926038 sequences (240000128 bp)... [M::process] 0 single-end sequences; 1926038 paired-end sequences [M::process] read 1925930 sequences (240000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1232, 752311, 50, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 105, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (120.43, 69.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.32, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 186, 3175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9471) [M::mem_pestat] mean and std.dev: (1550.00, 2110.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12619) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (79, 176, 700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1942) [M::mem_pestat] mean and std.dev: (342.37, 458.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2563) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1926038 reads in 993.906 CPU sec, 62.441 real sec [M::process] 0 single-end sequences; 1925930 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1219, 751949, 57, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 109, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (124.99, 71.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.10) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 163, 2687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7979) [M::mem_pestat] mean and std.dev: (1325.18, 1892.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10625) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (128, 257, 1152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3200) [M::mem_pestat] mean and std.dev: (528.33, 618.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4224) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925930 reads in 951.876 CPU sec, 42.037 real sec [M::process] read 1925922 sequences (240000112 bp)... [M::process] 0 single-end sequences; 1925922 paired-end sequences [M::process] read 1925914 sequences (240000244 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1300, 751913, 52, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 112, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (126.80, 73.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 177, 2888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8606) [M::mem_pestat] mean and std.dev: (1513.29, 2378.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11465) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (148, 219, 1012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2740) [M::mem_pestat] mean and std.dev: (440.18, 499.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3604) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925922 reads in 991.921 CPU sec, 64.487 real sec [M::process] 0 single-end sequences; 1925914 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1238, 752050, 56, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 115, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (126.73, 72.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 188, 2186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6494) [M::mem_pestat] mean and std.dev: (1264.73, 1781.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8648) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 205, 748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1972) [M::mem_pestat] mean and std.dev: (358.12, 406.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2584) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925914 reads in 956.916 CPU sec, 42.166 real sec [M::process] read 1925928 sequences (240000218 bp)... [M::process] 0 single-end sequences; 1925928 paired-end sequences [M::process] read 1925888 sequences (240000084 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1280, 752454, 57, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 105, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (121.42, 70.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.10) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 154, 1947) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5795) [M::mem_pestat] mean and std.dev: (994.38, 1523.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7719) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 208, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2361) [M::mem_pestat] mean and std.dev: (446.93, 457.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3101) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925928 reads in 1004.309 CPU sec, 63.692 real sec [M::process] 0 single-end sequences; 1925888 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1225, 751863, 38, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 108, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (123.12, 72.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 145, 2636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7854) [M::mem_pestat] mean and std.dev: (1296.74, 1990.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10463) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 217, 411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 911) [M::mem_pestat] mean and std.dev: (232.19, 141.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925888 reads in 957.505 CPU sec, 42.121 real sec [M::process] read 1925928 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1925928 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1298, 752107, 44, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 113, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (127.82, 72.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.33, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 188, 3055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9127) [M::mem_pestat] mean and std.dev: (1490.89, 1969.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12163) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (112, 171, 878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2410) [M::mem_pestat] mean and std.dev: (392.62, 525.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3176) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925928 reads in 996.187 CPU sec, 62.957 real sec [M::process] read 1925866 sequences (240000198 bp)... [M::process] 0 single-end sequences; 1925866 paired-end sequences [M::process] read 1925918 sequences (240000146 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1291, 751968, 47, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 115, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (129.10, 73.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.43, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 178, 3035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9047) [M::mem_pestat] mean and std.dev: (1304.94, 1742.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12053) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (61, 197, 817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2329) [M::mem_pestat] mean and std.dev: (390.19, 460.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3085) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925866 reads in 1008.171 CPU sec, 63.322 real sec [M::process] 0 single-end sequences; 1925918 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1234, 752010, 62, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 115, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (128.81, 75.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 403, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.48, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 122, 2616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7806) [M::mem_pestat] mean and std.dev: (1286.08, 1932.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10401) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (107, 181, 1033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2885) [M::mem_pestat] mean and std.dev: (477.00, 635.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3811) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925918 reads in 960.273 CPU sec, 42.875 real sec [M::process] read 1925872 sequences (240000142 bp)... [M::process] 0 single-end sequences; 1925872 paired-end sequences [M::process] read 1925892 sequences (240000032 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1271, 752209, 65, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 114, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (126.55, 73.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 90, 1651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4897) [M::mem_pestat] mean and std.dev: (850.68, 1384.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6520) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (117, 181, 637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1677) [M::mem_pestat] mean and std.dev: (276.33, 329.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2197) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925872 reads in 993.228 CPU sec, 64.338 real sec [M::process] 0 single-end sequences; 1925892 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1254, 751771, 40, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 106, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (122.31, 71.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 70, 874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2584) [M::mem_pestat] mean and std.dev: (248.15, 527.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3439) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (117, 244, 925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2541) [M::mem_pestat] mean and std.dev: (426.74, 508.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3349) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925892 reads in 959.003 CPU sec, 42.372 real sec [M::process] read 1925958 sequences (240000150 bp)... [M::process] 0 single-end sequences; 1925958 paired-end sequences [M::process] read 1925790 sequences (240000128 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1187, 751936, 54, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 108, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (122.53, 72.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 95, 1154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3416) [M::mem_pestat] mean and std.dev: (289.53, 653.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4547) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (104, 165, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 803) [M::mem_pestat] mean and std.dev: (172.36, 133.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1036) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925958 reads in 986.762 CPU sec, 64.393 real sec [M::process] 0 single-end sequences; 1925790 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1192, 752048, 40, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 113, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (125.60, 72.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 175, 1969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5833) [M::mem_pestat] mean and std.dev: (1116.03, 1551.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7765) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (119, 197, 647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1703) [M::mem_pestat] mean and std.dev: (349.59, 381.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2231) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925790 reads in 965.866 CPU sec, 42.659 real sec [M::process] read 1925996 sequences (240000226 bp)... [M::process] 0 single-end sequences; 1925996 paired-end sequences [M::process] read 1925922 sequences (240000070 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1267, 751844, 51, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 110, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (126.91, 75.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 159, 2636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7848) [M::mem_pestat] mean and std.dev: (1335.75, 1936.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10454) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (127, 217, 1307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3667) [M::mem_pestat] mean and std.dev: (587.83, 801.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4847) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925996 reads in 996.200 CPU sec, 64.279 real sec [M::process] 0 single-end sequences; 1925922 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1257, 752244, 48, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 109, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (124.77, 74.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.33, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 153, 4372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13062) [M::mem_pestat] mean and std.dev: (1377.19, 1921.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17407) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (120, 215, 986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2718) [M::mem_pestat] mean and std.dev: (478.60, 539.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3584) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925922 reads in 957.880 CPU sec, 42.135 real sec [M::process] read 1925930 sequences (240000210 bp)... [M::process] 0 single-end sequences; 1925930 paired-end sequences [M::process] read 1925944 sequences (240000032 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1227, 752016, 51, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 108, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (125.61, 72.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.42, 30.09) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 164, 1997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5933) [M::mem_pestat] mean and std.dev: (1019.77, 1528.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7901) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (130, 199, 710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1870) [M::mem_pestat] mean and std.dev: (331.76, 370.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2450) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925930 reads in 999.601 CPU sec, 64.592 real sec [M::process] 0 single-end sequences; 1925944 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1256, 752295, 52, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 114, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (125.01, 71.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.33, 30.10) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 128, 2062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6128) [M::mem_pestat] mean and std.dev: (932.08, 1631.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8161) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (116, 187, 706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1886) [M::mem_pestat] mean and std.dev: (385.33, 456.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2476) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925944 reads in 955.415 CPU sec, 42.071 real sec [M::process] read 1925884 sequences (240000112 bp)... [M::process] 0 single-end sequences; 1925884 paired-end sequences [M::process] read 1925932 sequences (240000182 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1265, 751722, 41, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 115, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (130.27, 73.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 168, 2678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7966) [M::mem_pestat] mean and std.dev: (1144.56, 1557.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10610) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 213, 842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2244) [M::mem_pestat] mean and std.dev: (379.68, 434.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2945) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925884 reads in 979.617 CPU sec, 62.519 real sec [M::process] 0 single-end sequences; 1925932 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1233, 751609, 46, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 106, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (122.57, 71.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 143, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1329) [M::mem_pestat] mean and std.dev: (159.18, 224.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1765) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (125, 231, 948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2594) [M::mem_pestat] mean and std.dev: (484.51, 536.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3417) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925932 reads in 968.648 CPU sec, 44.050 real sec [M::process] read 1925930 sequences (240000026 bp)... [M::process] 0 single-end sequences; 1925930 paired-end sequences [M::process] read 1925960 sequences (240000168 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1335, 752121, 55, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 110, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (124.78, 75.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.42, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (61, 149, 3045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9013) [M::mem_pestat] mean and std.dev: (1430.64, 2161.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11997) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (115, 194, 750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2020) [M::mem_pestat] mean and std.dev: (374.72, 434.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2655) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925930 reads in 982.703 CPU sec, 64.094 real sec [M::process] 0 single-end sequences; 1925960 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1171, 752014, 49, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 106, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (124.55, 74.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.22) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 161, 4370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13028) [M::mem_pestat] mean and std.dev: (1578.18, 2242.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17357) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (63, 169, 767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2175) [M::mem_pestat] mean and std.dev: (366.41, 463.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2879) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925960 reads in 964.360 CPU sec, 42.600 real sec [M::process] read 1925964 sequences (240000060 bp)... [M::process] 0 single-end sequences; 1925964 paired-end sequences [M::process] read 1925954 sequences (240000200 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1264, 751807, 44, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 108, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (123.34, 73.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.09) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 62, 1988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5920) [M::mem_pestat] mean and std.dev: (862.74, 1450.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7886) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (102, 185, 1132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3192) [M::mem_pestat] mean and std.dev: (518.03, 763.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4222) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925964 reads in 980.090 CPU sec, 64.766 real sec [M::process] 0 single-end sequences; 1925954 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1203, 751989, 58, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 111, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (126.00, 74.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 176, 2143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6387) [M::mem_pestat] mean and std.dev: (1136.80, 1647.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8509) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (78, 218, 836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2352) [M::mem_pestat] mean and std.dev: (489.25, 573.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3110) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925954 reads in 960.494 CPU sec, 42.311 real sec [M::process] read 1925882 sequences (240000212 bp)... [M::process] 0 single-end sequences; 1925882 paired-end sequences [M::process] read 1925888 sequences (240000078 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1212, 751989, 41, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 109, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (123.07, 70.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 84, 1096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3240) [M::mem_pestat] mean and std.dev: (362.36, 715.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4312) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (117, 191, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (182.51, 148.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925882 reads in 994.512 CPU sec, 63.584 real sec [M::process] 0 single-end sequences; 1925888 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1319, 752343, 38, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 105, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (123.68, 73.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 151, 1584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4694) [M::mem_pestat] mean and std.dev: (825.49, 1264.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6249) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (101, 186, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2312) [M::mem_pestat] mean and std.dev: (371.18, 451.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3049) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925888 reads in 958.118 CPU sec, 42.097 real sec [M::process] read 1925942 sequences (240000076 bp)... [M::process] 0 single-end sequences; 1925942 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1244, 752025, 45, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 111, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (124.01, 72.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 96, 3538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10570) [M::mem_pestat] mean and std.dev: (1693.87, 2369.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14086) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (152, 233, 708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1820) [M::mem_pestat] mean and std.dev: (433.17, 483.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2376) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925942 reads in 995.995 CPU sec, 57.919 real sec [M::process] read 1925954 sequences (240000104 bp)... [M::process] 0 single-end sequences; 1925954 paired-end sequences [M::process] read 1925846 sequences (240000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1268, 752482, 36, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 109, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (125.64, 74.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.11) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 52, 1128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3332) [M::mem_pestat] mean and std.dev: (319.03, 652.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4434) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (83, 172, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (146.42, 124.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 959) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925954 reads in 1008.006 CPU sec, 63.113 real sec [M::process] 0 single-end sequences; 1925846 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1243, 752020, 42, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 109, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (124.51, 74.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 66, 512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1502) [M::mem_pestat] mean and std.dev: (154.09, 295.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1997) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 252, 1346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3706) [M::mem_pestat] mean and std.dev: (632.78, 657.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4886) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925846 reads in 962.308 CPU sec, 43.862 real sec [M::process] read 1925912 sequences (240000112 bp)... [M::process] 0 single-end sequences; 1925912 paired-end sequences [M::process] read 1925964 sequences (240000228 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1229, 753010, 48, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 108, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (123.83, 72.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 159, 2976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8886) [M::mem_pestat] mean and std.dev: (1552.27, 2107.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11841) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (90, 166, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1248) [M::mem_pestat] mean and std.dev: (249.00, 289.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1634) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925912 reads in 1001.381 CPU sec, 63.378 real sec [M::process] 0 single-end sequences; 1925964 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1204, 751918, 52, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 111, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (125.46, 73.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 150, 2584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7690) [M::mem_pestat] mean and std.dev: (1190.65, 1746.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10243) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (83, 217, 755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2099) [M::mem_pestat] mean and std.dev: (373.44, 476.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2771) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925964 reads in 957.784 CPU sec, 42.285 real sec [M::process] read 1925932 sequences (240000100 bp)... [M::process] 0 single-end sequences; 1925932 paired-end sequences [M::process] read 1925918 sequences (240000206 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1274, 751647, 43, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 115, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (128.42, 74.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 62, 1590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4748) [M::mem_pestat] mean and std.dev: (911.43, 1590.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7273) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (98, 190, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (180.21, 164.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1122) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925932 reads in 1008.166 CPU sec, 63.560 real sec [M::process] 0 single-end sequences; 1925918 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1196, 752131, 50, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 113, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (124.03, 72.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 125, 2610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7782) [M::mem_pestat] mean and std.dev: (1332.76, 1919.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10368) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (132, 244, 1176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3264) [M::mem_pestat] mean and std.dev: (586.04, 644.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4308) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925918 reads in 957.747 CPU sec, 42.169 real sec [M::process] read 1925908 sequences (240000096 bp)... [M::process] 0 single-end sequences; 1925908 paired-end sequences [M::process] read 1925900 sequences (240000222 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1228, 752288, 64, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 116, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (128.67, 72.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 187, 2541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7549) [M::mem_pestat] mean and std.dev: (1304.30, 1822.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10053) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (93, 172, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (188.37, 181.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1189) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925908 reads in 990.846 CPU sec, 63.453 real sec [M::process] 0 single-end sequences; 1925900 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1237, 751985, 55, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 107, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (122.07, 72.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 170, 1804) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5338) [M::mem_pestat] mean and std.dev: (1008.57, 1468.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7105) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (69, 127, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (130.85, 85.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925900 reads in 952.904 CPU sec, 41.888 real sec [M::process] read 1925888 sequences (240000154 bp)... [M::process] 0 single-end sequences; 1925888 paired-end sequences [M::process] read 1925934 sequences (240000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1258, 752282, 46, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 113, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (125.54, 72.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 208, 4374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13050) [M::mem_pestat] mean and std.dev: (1674.83, 2347.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17388) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (103, 182, 988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2758) [M::mem_pestat] mean and std.dev: (457.48, 612.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3643) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925888 reads in 991.785 CPU sec, 64.943 real sec [M::process] 0 single-end sequences; 1925934 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1313, 750775, 48, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 109, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (124.42, 73.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (66, 477, 4372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12984) [M::mem_pestat] mean and std.dev: (1740.44, 2088.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17290) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (109, 235, 663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1771) [M::mem_pestat] mean and std.dev: (331.76, 363.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2325) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925934 reads in 966.001 CPU sec, 42.519 real sec [M::process] read 1926034 sequences (240000064 bp)... [M::process] 0 single-end sequences; 1926034 paired-end sequences [M::process] read 1925786 sequences (240000244 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1222, 752597, 47, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 108, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (122.97, 70.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 501) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (67, 194, 4071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12079) [M::mem_pestat] mean and std.dev: (1547.23, 2219.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16083) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (105, 211, 1049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2937) [M::mem_pestat] mean and std.dev: (550.64, 682.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3881) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1926034 reads in 990.813 CPU sec, 62.645 real sec [M::process] 0 single-end sequences; 1925786 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1285, 752136, 44, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 111, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (125.18, 72.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 181, 2002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5930) [M::mem_pestat] mean and std.dev: (994.14, 1574.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7894) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (125, 242, 904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2462) [M::mem_pestat] mean and std.dev: (410.29, 431.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3241) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925786 reads in 962.512 CPU sec, 43.790 real sec [M::process] read 1925972 sequences (240000192 bp)... [M::process] 0 single-end sequences; 1925972 paired-end sequences [M::process] read 1925970 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1270, 752454, 67, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 106, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (123.36, 73.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 136, 2677) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7963) [M::mem_pestat] mean and std.dev: (1155.80, 1808.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10606) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (118, 193, 1015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2809) [M::mem_pestat] mean and std.dev: (397.64, 478.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3706) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925972 reads in 998.344 CPU sec, 63.257 real sec [M::process] 0 single-end sequences; 1925970 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1210, 751810, 48, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 109, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (123.05, 72.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.10) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 171, 2537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7557) [M::mem_pestat] mean and std.dev: (1182.61, 1592.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10067) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (74, 164, 809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2279) [M::mem_pestat] mean and std.dev: (342.14, 458.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3014) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925970 reads in 961.112 CPU sec, 42.378 real sec [M::process] read 1925858 sequences (240000140 bp)... [M::process] 0 single-end sequences; 1925858 paired-end sequences [M::process] read 1925956 sequences (240000184 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1290, 752101, 42, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 107, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (122.50, 73.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 161, 2621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7817) [M::mem_pestat] mean and std.dev: (1274.90, 1894.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10415) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (111, 224, 1249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3525) [M::mem_pestat] mean and std.dev: (567.19, 658.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4663) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925858 reads in 994.791 CPU sec, 63.629 real sec [M::process] 0 single-end sequences; 1925956 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1252, 752354, 40, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 110, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (125.86, 73.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (61, 167, 2704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7990) [M::mem_pestat] mean and std.dev: (1251.10, 1820.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10633) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (109, 211, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1012) [M::mem_pestat] mean and std.dev: (223.02, 195.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1313) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925956 reads in 958.548 CPU sec, 42.164 real sec [M::process] read 1925868 sequences (240000028 bp)... [M::process] 0 single-end sequences; 1925868 paired-end sequences [M::process] read 1925860 sequences (240000192 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1210, 752034, 56, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 112, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (125.67, 73.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 228, 1999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5941) [M::mem_pestat] mean and std.dev: (1110.26, 1605.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7912) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (125, 191, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (170.67, 92.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 785) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925868 reads in 998.260 CPU sec, 63.189 real sec [M::process] 0 single-end sequences; 1925860 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1244, 751628, 54, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 104, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (120.35, 71.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 60, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1190) [M::mem_pestat] mean and std.dev: (105.21, 201.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1582) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (104, 191, 751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2045) [M::mem_pestat] mean and std.dev: (309.76, 444.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2692) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925860 reads in 960.804 CPU sec, 42.280 real sec [M::process] read 1925898 sequences (240000194 bp)... [M::process] 0 single-end sequences; 1925898 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1243, 752036, 47, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 110, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (125.65, 73.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 242, 3193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9535) [M::mem_pestat] mean and std.dev: (1466.96, 1851.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12706) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (128, 207, 998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2738) [M::mem_pestat] mean and std.dev: (545.98, 686.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3608) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925898 reads in 977.476 CPU sec, 54.331 real sec [M::process] read 1925898 sequences (240000170 bp)... [M::process] 0 single-end sequences; 1925898 paired-end sequences [M::process] read 1925960 sequences (240000226 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1254, 751811, 46, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 114, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (125.41, 72.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 115, 611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1783) [M::mem_pestat] mean and std.dev: (214.85, 356.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2369) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (99, 183, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (175.31, 137.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1003) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925898 reads in 992.503 CPU sec, 62.283 real sec [M::process] 0 single-end sequences; 1925960 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1275, 752410, 41, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 114, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (126.44, 74.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 57, 1330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3960) [M::mem_pestat] mean and std.dev: (373.06, 782.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5275) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (109, 185, 1056) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2950) [M::mem_pestat] mean and std.dev: (560.62, 729.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3897) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925960 reads in 962.025 CPU sec, 43.704 real sec [M::process] read 1925898 sequences (240000030 bp)... [M::process] 0 single-end sequences; 1925898 paired-end sequences [M::process] read 1925918 sequences (240000162 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1225, 751874, 51, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 117, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (129.60, 73.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 156, 1511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4459) [M::mem_pestat] mean and std.dev: (784.28, 1314.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6042) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 228, 1388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3850) [M::mem_pestat] mean and std.dev: (516.72, 582.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5081) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925898 reads in 1007.969 CPU sec, 63.978 real sec [M::process] 0 single-end sequences; 1925918 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1266, 751589, 42, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 111, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (125.17, 72.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.08) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 72, 2446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7314) [M::mem_pestat] mean and std.dev: (1026.90, 1593.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9748) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (119, 193, 1106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3080) [M::mem_pestat] mean and std.dev: (490.52, 662.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4067) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925918 reads in 952.726 CPU sec, 42.057 real sec [M::process] read 1925908 sequences (240000240 bp)... [M::process] 0 single-end sequences; 1925908 paired-end sequences [M::process] read 1925836 sequences (240000192 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1301, 751322, 44, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 106, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (122.63, 72.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 135, 1671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4959) [M::mem_pestat] mean and std.dev: (925.24, 1482.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6856) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 237, 1193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3253) [M::mem_pestat] mean and std.dev: (520.51, 579.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4283) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925908 reads in 995.778 CPU sec, 65.331 real sec [M::process] 0 single-end sequences; 1925836 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1271, 752551, 40, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 108, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (122.84, 73.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.33, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 140, 1497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4455) [M::mem_pestat] mean and std.dev: (815.54, 1279.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5934) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (122, 209, 1109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3083) [M::mem_pestat] mean and std.dev: (634.56, 785.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4070) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925836 reads in 953.704 CPU sec, 42.015 real sec [M::process] read 1925888 sequences (240000238 bp)... [M::process] 0 single-end sequences; 1925888 paired-end sequences [M::process] read 1925914 sequences (240000108 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1262, 752302, 66, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 110, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (125.58, 72.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 88, 864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2544) [M::mem_pestat] mean and std.dev: (156.46, 311.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3384) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (121, 212, 1039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2875) [M::mem_pestat] mean and std.dev: (530.14, 612.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3793) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925888 reads in 1004.471 CPU sec, 64.184 real sec [M::process] 0 single-end sequences; 1925914 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1258, 752134, 45, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 110, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (127.07, 72.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 91, 1603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4767) [M::mem_pestat] mean and std.dev: (653.00, 1202.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6349) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 236, 1193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3301) [M::mem_pestat] mean and std.dev: (607.05, 735.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4355) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925914 reads in 960.174 CPU sec, 42.224 real sec [M::process] read 1925832 sequences (240000144 bp)... [M::process] 0 single-end sequences; 1925832 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1240, 751571, 45, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 110, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (125.71, 73.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.11) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 147, 2550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7570) [M::mem_pestat] mean and std.dev: (1306.71, 1837.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10080) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (166, 234, 691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1741) [M::mem_pestat] mean and std.dev: (328.82, 299.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2266) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925832 reads in 1004.970 CPU sec, 65.060 real sec [M::process] read 1925938 sequences (240000180 bp)... [M::process] 0 single-end sequences; 1925938 paired-end sequences [M::process] read 1925894 sequences (240000098 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1301, 751996, 60, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 111, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (126.12, 73.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.10) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 144, 1323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3911) [M::mem_pestat] mean and std.dev: (456.61, 843.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5205) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 258, 853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2287) [M::mem_pestat] mean and std.dev: (435.10, 463.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3004) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925938 reads in 998.785 CPU sec, 62.442 real sec [M::process] 0 single-end sequences; 1925894 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1291, 752547, 45, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 112, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (124.22, 72.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 148, 2643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7867) [M::mem_pestat] mean and std.dev: (991.88, 1607.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10479) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (126, 224, 842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2274) [M::mem_pestat] mean and std.dev: (429.22, 514.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2990) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925894 reads in 962.607 CPU sec, 43.658 real sec [M::process] read 1925932 sequences (240000210 bp)... [M::process] 0 single-end sequences; 1925932 paired-end sequences [M::process] read 1925920 sequences (240000120 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1233, 752072, 48, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 106, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (124.04, 74.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (64, 172, 4214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12514) [M::mem_pestat] mean and std.dev: (1966.19, 2631.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16664) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (156, 241, 985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2643) [M::mem_pestat] mean and std.dev: (504.76, 495.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3472) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925932 reads in 1003.932 CPU sec, 62.997 real sec [M::process] 0 single-end sequences; 1925920 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1229, 751815, 47, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 108, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (123.85, 73.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 120, 1446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4258) [M::mem_pestat] mean and std.dev: (477.39, 910.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5664) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (111, 198, 1075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3003) [M::mem_pestat] mean and std.dev: (506.64, 642.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3967) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925920 reads in 962.822 CPU sec, 42.502 real sec [M::process] read 1925942 sequences (240000244 bp)... [M::process] 0 single-end sequences; 1925942 paired-end sequences [M::process] read 1925998 sequences (240000140 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1239, 752206, 58, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 111, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (124.71, 72.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 148, 3224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9610) [M::mem_pestat] mean and std.dev: (1400.81, 1929.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12803) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (186, 473, 1028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2712) [M::mem_pestat] mean and std.dev: (583.08, 520.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3554) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925942 reads in 984.615 CPU sec, 62.647 real sec [M::process] 0 single-end sequences; 1925998 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1272, 751696, 43, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 110, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (123.23, 71.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (60, 158, 3496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10368) [M::mem_pestat] mean and std.dev: (1359.09, 2073.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13804) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (124, 226, 1030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2842) [M::mem_pestat] mean and std.dev: (518.80, 621.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3748) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925998 reads in 964.932 CPU sec, 43.993 real sec [M::process] read 1925950 sequences (240000114 bp)... [M::process] 0 single-end sequences; 1925950 paired-end sequences [M::process] read 1925932 sequences (240000184 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1270, 751811, 45, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 113, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (126.25, 71.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 188, 3850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11476) [M::mem_pestat] mean and std.dev: (1750.42, 2500.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15289) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 235, 741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1943) [M::mem_pestat] mean and std.dev: (336.47, 365.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2544) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925950 reads in 989.603 CPU sec, 64.549 real sec [M::process] 0 single-end sequences; 1925932 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1235, 752351, 33, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 116, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (127.60, 73.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.20) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 163, 4391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13117) [M::mem_pestat] mean and std.dev: (1411.24, 2011.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17480) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (85, 231, 1078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3064) [M::mem_pestat] mean and std.dev: (603.27, 672.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4057) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925932 reads in 954.410 CPU sec, 42.138 real sec [M::process] read 1925912 sequences (240000014 bp)... [M::process] 0 single-end sequences; 1925912 paired-end sequences [M::process] read 1926000 sequences (240000186 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1218, 752103, 51, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 112, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (125.25, 72.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 146, 2372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7070) [M::mem_pestat] mean and std.dev: (1130.54, 1642.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9419) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (137, 229, 958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2600) [M::mem_pestat] mean and std.dev: (551.98, 674.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3421) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925912 reads in 999.356 CPU sec, 64.209 real sec [M::process] 0 single-end sequences; 1926000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1169, 752257, 43, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 117, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (127.21, 74.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.44, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 23, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1095) [M::mem_pestat] mean and std.dev: (60.29, 123.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1457) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (77, 158, 674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1868) [M::mem_pestat] mean and std.dev: (289.84, 379.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2465) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1926000 reads in 950.557 CPU sec, 41.843 real sec [M::process] read 1925922 sequences (240000008 bp)... [M::process] 0 single-end sequences; 1925922 paired-end sequences [M::process] read 1925962 sequences (240000224 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1275, 751909, 53, 59) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 114, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (127.56, 74.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 231, 4376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13052) [M::mem_pestat] mean and std.dev: (1942.66, 2370.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17390) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (104, 241, 1075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3017) [M::mem_pestat] mean and std.dev: (560.93, 677.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3988) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925922 reads in 994.255 CPU sec, 61.797 real sec [M::process] 0 single-end sequences; 1925962 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1227, 751056, 39, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 108, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (124.39, 73.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.44, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 131, 597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1757) [M::mem_pestat] mean and std.dev: (140.61, 179.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2337) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (130, 194, 1031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2833) [M::mem_pestat] mean and std.dev: (522.93, 600.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3734) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925962 reads in 970.189 CPU sec, 44.038 real sec [M::process] read 1925818 sequences (240000208 bp)... [M::process] 0 single-end sequences; 1925818 paired-end sequences [M::process] read 1925868 sequences (240000128 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1310, 751206, 48, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 107, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 389) [M::mem_pestat] mean and std.dev: (121.33, 70.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 497) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.21) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 203, 3661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10953) [M::mem_pestat] mean and std.dev: (1947.60, 2717.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14599) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (117, 199, 815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2211) [M::mem_pestat] mean and std.dev: (409.90, 496.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2909) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925818 reads in 993.963 CPU sec, 63.661 real sec [M::process] 0 single-end sequences; 1925868 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1296, 752150, 45, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 112, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (123.90, 72.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.11) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (51, 179, 3497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10389) [M::mem_pestat] mean and std.dev: (1605.40, 2360.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13835) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (64, 137, 732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2068) [M::mem_pestat] mean and std.dev: (340.15, 469.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2736) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925868 reads in 968.030 CPU sec, 43.692 real sec [M::process] read 1925876 sequences (240000036 bp)... [M::process] 0 single-end sequences; 1925876 paired-end sequences [M::process] read 1925902 sequences (240000056 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1227, 752721, 56, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 109, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (123.32, 69.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 499) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 59, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (112.39, 177.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1262) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (126, 284, 1006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2766) [M::mem_pestat] mean and std.dev: (572.39, 556.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3646) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925876 reads in 999.122 CPU sec, 63.792 real sec [M::process] 0 single-end sequences; 1925902 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1277, 751974, 41, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 119, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (130.65, 74.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 95, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3421) [M::mem_pestat] mean and std.dev: (595.82, 975.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4553) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (114, 190, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 555) [M::mem_pestat] mean and std.dev: (148.62, 76.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 702) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925902 reads in 962.471 CPU sec, 42.464 real sec [M::process] read 1925902 sequences (240000134 bp)... [M::process] 0 single-end sequences; 1925902 paired-end sequences [M::process] read 1925886 sequences (240000148 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1226, 751845, 56, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 110, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (125.40, 72.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 82, 1466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4354) [M::mem_pestat] mean and std.dev: (527.46, 1021.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5798) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (128, 205, 914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2486) [M::mem_pestat] mean and std.dev: (447.20, 508.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3272) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925902 reads in 1002.861 CPU sec, 63.968 real sec [M::process] 0 single-end sequences; 1925886 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1161, 751665, 46, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 114, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (128.42, 76.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 74, 544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1594) [M::mem_pestat] mean and std.dev: (132.84, 257.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2119) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (87, 292, 1075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3051) [M::mem_pestat] mean and std.dev: (654.33, 810.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4039) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925886 reads in 949.886 CPU sec, 41.780 real sec [M::process] read 1925908 sequences (240000198 bp)... [M::process] 0 single-end sequences; 1925908 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1216, 751934, 42, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 106, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (124.13, 73.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.33, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 159, 707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2081) [M::mem_pestat] mean and std.dev: (274.33, 420.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2768) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (113, 213, 871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2387) [M::mem_pestat] mean and std.dev: (407.87, 497.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3145) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925908 reads in 987.222 CPU sec, 60.802 real sec [M::process] read 1925806 sequences (240000118 bp)... [M::process] 0 single-end sequences; 1925806 paired-end sequences [M::process] read 1925928 sequences (240000052 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1257, 751111, 58, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 109, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (124.78, 73.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 154, 3189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9503) [M::mem_pestat] mean and std.dev: (1506.03, 1931.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12660) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (111, 191, 964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2670) [M::mem_pestat] mean and std.dev: (492.68, 670.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3523) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925806 reads in 993.130 CPU sec, 62.742 real sec [M::process] 0 single-end sequences; 1925928 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1327, 752037, 41, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 113, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (123.14, 70.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.22) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 438, 3645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10841) [M::mem_pestat] mean and std.dev: (1608.10, 2095.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14439) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (102, 175, 1133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3195) [M::mem_pestat] mean and std.dev: (512.71, 584.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4226) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925928 reads in 968.102 CPU sec, 43.864 real sec [M::process] read 1925878 sequences (240000134 bp)... [M::process] 0 single-end sequences; 1925878 paired-end sequences [M::process] read 1925894 sequences (240000052 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1204, 751724, 55, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 113, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (124.09, 72.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.11) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 162, 2641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7849) [M::mem_pestat] mean and std.dev: (1190.57, 1797.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10453) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (114, 207, 1051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2925) [M::mem_pestat] mean and std.dev: (567.39, 743.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3862) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925878 reads in 998.035 CPU sec, 63.597 real sec [M::process] 0 single-end sequences; 1925894 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1235, 751587, 42, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 117, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (127.86, 73.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.11) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 147, 1972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5836) [M::mem_pestat] mean and std.dev: (995.22, 1570.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7768) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (134, 254, 1035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2837) [M::mem_pestat] mean and std.dev: (538.86, 543.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3738) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925894 reads in 964.195 CPU sec, 42.657 real sec [M::process] read 1925822 sequences (240000006 bp)... [M::process] 0 single-end sequences; 1925822 paired-end sequences [M::process] read 1925908 sequences (240000242 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1190, 751940, 32, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 105, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 385) [M::mem_pestat] mean and std.dev: (120.84, 70.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 82, 1776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5284) [M::mem_pestat] mean and std.dev: (840.13, 1411.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7038) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (89, 192, 1564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4514) [M::mem_pestat] mean and std.dev: (528.78, 635.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5989) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925822 reads in 989.597 CPU sec, 65.080 real sec [M::process] 0 single-end sequences; 1925908 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1242, 751151, 54, 60) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 115, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (128.22, 72.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.44, 30.10) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 147, 3667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10939) [M::mem_pestat] mean and std.dev: (1487.80, 2316.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14575) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (125, 218, 923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2519) [M::mem_pestat] mean and std.dev: (447.39, 558.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925908 reads in 966.003 CPU sec, 42.526 real sec [M::process] read 1925776 sequences (240000194 bp)... [M::process] 0 single-end sequences; 1925776 paired-end sequences [M::process] read 1925970 sequences (240000242 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1231, 751967, 64, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 116, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (127.99, 73.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.42, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 130, 1228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3618) [M::mem_pestat] mean and std.dev: (404.15, 755.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4813) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (106, 187, 681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1831) [M::mem_pestat] mean and std.dev: (387.53, 447.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2406) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925776 reads in 994.341 CPU sec, 64.614 real sec [M::process] 0 single-end sequences; 1925970 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1241, 751817, 39, 29) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 108, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (121.87, 70.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 147, 2618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7800) [M::mem_pestat] mean and std.dev: (1163.26, 1778.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10391) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (109, 185, 879) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2419) [M::mem_pestat] mean and std.dev: (418.44, 513.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3189) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925970 reads in 951.114 CPU sec, 41.815 real sec [M::process] read 1925824 sequences (240000064 bp)... [M::process] 0 single-end sequences; 1925824 paired-end sequences [M::process] read 1925882 sequences (240000226 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1225, 751541, 43, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 107, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (123.70, 74.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 146, 4397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13143) [M::mem_pestat] mean and std.dev: (1980.16, 2533.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17516) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (79, 157, 788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2206) [M::mem_pestat] mean and std.dev: (348.61, 490.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2915) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925824 reads in 995.429 CPU sec, 65.124 real sec [M::process] 0 single-end sequences; 1925882 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1286, 751601, 54, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 110, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (123.73, 72.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 191, 4396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13110) [M::mem_pestat] mean and std.dev: (1986.44, 2729.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17467) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 235, 1203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3325) [M::mem_pestat] mean and std.dev: (506.00, 606.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4386) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925882 reads in 962.706 CPU sec, 42.278 real sec [M::process] read 1925870 sequences (240000120 bp)... [M::process] 0 single-end sequences; 1925870 paired-end sequences [M::process] read 1925896 sequences (240000136 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1244, 751002, 58, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 111, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (125.40, 71.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.11) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 178, 3782) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11300) [M::mem_pestat] mean and std.dev: (1909.43, 2711.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15059) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (67, 134, 738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2080) [M::mem_pestat] mean and std.dev: (238.19, 387.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2751) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925870 reads in 998.032 CPU sec, 62.292 real sec [M::process] 0 single-end sequences; 1925896 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1232, 752071, 47, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 109, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (123.73, 73.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.43, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 100, 2645) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7905) [M::mem_pestat] mean and std.dev: (1041.51, 1647.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10535) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (79, 211, 758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2116) [M::mem_pestat] mean and std.dev: (387.82, 505.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2795) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925896 reads in 966.730 CPU sec, 44.040 real sec [M::process] read 1925790 sequences (240000046 bp)... [M::process] 0 single-end sequences; 1925790 paired-end sequences [M::process] read 1925906 sequences (240000048 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1205, 751448, 41, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 106, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (122.93, 74.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (92, 587, 4375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12941) [M::mem_pestat] mean and std.dev: (1853.80, 2334.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17224) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 268, 1308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3640) [M::mem_pestat] mean and std.dev: (731.88, 903.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4806) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925790 reads in 992.477 CPU sec, 62.974 real sec [M::process] 0 single-end sequences; 1925906 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1261, 751891, 55, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 105, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (122.09, 73.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.45, 30.10) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 136, 4379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13071) [M::mem_pestat] mean and std.dev: (1657.27, 2364.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17417) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (108, 182, 747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2025) [M::mem_pestat] mean and std.dev: (342.49, 424.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2664) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925906 reads in 956.571 CPU sec, 42.236 real sec [M::process] read 1925830 sequences (240000188 bp)... [M::process] 0 single-end sequences; 1925830 paired-end sequences [M::process] read 1925966 sequences (240000138 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1276, 751194, 50, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 108, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (124.20, 72.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 175, 2906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8676) [M::mem_pestat] mean and std.dev: (1359.24, 1915.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11561) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 277, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2573) [M::mem_pestat] mean and std.dev: (554.58, 619.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3377) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925830 reads in 995.297 CPU sec, 62.731 real sec [M::process] 0 single-end sequences; 1925966 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1231, 752214, 44, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 105, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (122.72, 73.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (59, 184, 2802) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8288) [M::mem_pestat] mean and std.dev: (1340.28, 1908.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11031) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (92, 192, 339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 833) [M::mem_pestat] mean and std.dev: (179.30, 154.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1080) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925966 reads in 965.345 CPU sec, 42.477 real sec [M::process] read 1925878 sequences (240000196 bp)... [M::process] 0 single-end sequences; 1925878 paired-end sequences [M::process] read 1925950 sequences (240000230 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1287, 752783, 50, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 107, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (122.61, 72.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.42, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 200, 2820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8380) [M::mem_pestat] mean and std.dev: (1461.18, 2145.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11160) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (104, 208, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1394) [M::mem_pestat] mean and std.dev: (313.35, 321.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1824) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925878 reads in 982.625 CPU sec, 64.263 real sec [M::process] 0 single-end sequences; 1925950 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1239, 752111, 48, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 114, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (126.28, 72.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 155, 3688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11012) [M::mem_pestat] mean and std.dev: (1807.90, 2544.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14674) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (106, 188, 763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2077) [M::mem_pestat] mean and std.dev: (364.00, 386.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2734) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925950 reads in 953.889 CPU sec, 41.913 real sec [M::process] read 1925886 sequences (240000230 bp)... [M::process] 0 single-end sequences; 1925886 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1267, 752446, 70, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 113, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (126.75, 75.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.42, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 171, 2667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7937) [M::mem_pestat] mean and std.dev: (1118.38, 1670.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10572) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (89, 183, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (153.84, 110.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 797) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925886 reads in 988.030 CPU sec, 57.448 real sec [M::process] read 1925918 sequences (240000130 bp)... [M::process] 0 single-end sequences; 1925918 paired-end sequences [M::process] read 1925906 sequences (240000202 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1206, 751786, 53, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 113, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (127.89, 73.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 159, 2615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7777) [M::mem_pestat] mean and std.dev: (1178.78, 1691.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10358) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (118, 204, 1347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3805) [M::mem_pestat] mean and std.dev: (625.98, 713.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5034) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925918 reads in 996.669 CPU sec, 62.438 real sec [M::process] 0 single-end sequences; 1925906 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1214, 751918, 44, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 108, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (125.10, 74.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 148, 1765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5249) [M::mem_pestat] mean and std.dev: (986.00, 1581.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7312) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (106, 167, 1197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3379) [M::mem_pestat] mean and std.dev: (580.42, 842.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4470) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925906 reads in 960.977 CPU sec, 43.961 real sec [M::process] read 1925860 sequences (240000108 bp)... [M::process] 0 single-end sequences; 1925860 paired-end sequences [M::process] read 1925928 sequences (240000092 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1230, 751398, 38, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 113, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (127.88, 73.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 211, 4367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13057) [M::mem_pestat] mean and std.dev: (1582.50, 2004.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17402) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (174, 237, 1169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3159) [M::mem_pestat] mean and std.dev: (523.89, 659.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4154) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925860 reads in 990.300 CPU sec, 64.581 real sec [M::process] 0 single-end sequences; 1925928 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1218, 752275, 53, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 112, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (125.56, 74.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 90, 4385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13115) [M::mem_pestat] mean and std.dev: (1526.00, 2231.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17480) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (128, 196, 1165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3239) [M::mem_pestat] mean and std.dev: (541.70, 652.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4276) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925928 reads in 955.870 CPU sec, 42.291 real sec [M::process] read 1925926 sequences (240000134 bp)... [M::process] 0 single-end sequences; 1925926 paired-end sequences [M::process] read 1925936 sequences (240000198 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1235, 752355, 50, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 112, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (125.98, 73.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 195, 1795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5319) [M::mem_pestat] mean and std.dev: (900.59, 1435.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7081) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 253, 1261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3495) [M::mem_pestat] mean and std.dev: (642.53, 794.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4612) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925926 reads in 989.475 CPU sec, 65.500 real sec [M::process] 0 single-end sequences; 1925936 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1241, 752135, 56, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 112, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (126.99, 75.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.42, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 120, 1525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4529) [M::mem_pestat] mean and std.dev: (621.22, 1108.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6031) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (83, 212, 1177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3365) [M::mem_pestat] mean and std.dev: (640.02, 867.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4459) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925936 reads in 962.026 CPU sec, 42.388 real sec [M::process] read 1925928 sequences (240000110 bp)... [M::process] 0 single-end sequences; 1925928 paired-end sequences [M::process] read 1925962 sequences (240000178 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1275, 751879, 53, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 112, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (125.63, 71.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.20) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 88, 3177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9497) [M::mem_pestat] mean and std.dev: (1544.19, 2322.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12657) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (121, 214, 1067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2959) [M::mem_pestat] mean and std.dev: (482.00, 590.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3905) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925928 reads in 992.198 CPU sec, 63.981 real sec [M::process] 0 single-end sequences; 1925962 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1285, 751865, 41, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 108, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (124.36, 71.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.31, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 115, 1968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5860) [M::mem_pestat] mean and std.dev: (1109.05, 1620.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7806) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (121, 207, 962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2644) [M::mem_pestat] mean and std.dev: (509.61, 690.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3485) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925962 reads in 958.950 CPU sec, 42.344 real sec [M::process] read 1925908 sequences (240000050 bp)... [M::process] 0 single-end sequences; 1925908 paired-end sequences [M::process] read 1925838 sequences (240000024 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1241, 752279, 40, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 112, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (127.36, 72.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 175, 3818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11400) [M::mem_pestat] mean and std.dev: (1539.17, 1864.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15191) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 218, 743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1929) [M::mem_pestat] mean and std.dev: (418.32, 469.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2522) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925908 reads in 1002.075 CPU sec, 65.216 real sec [M::process] 0 single-end sequences; 1925838 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1293, 752252, 44, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 112, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (126.53, 74.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 146, 1972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5862) [M::mem_pestat] mean and std.dev: (923.29, 1403.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7807) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (190, 638, 1493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4099) [M::mem_pestat] mean and std.dev: (918.05, 955.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5402) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925838 reads in 952.363 CPU sec, 41.860 real sec [M::process] read 1925874 sequences (240000056 bp)... [M::process] 0 single-end sequences; 1925874 paired-end sequences [M::process] read 1925988 sequences (240000216 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1235, 751499, 47, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 113, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (127.05, 74.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 136, 1459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4327) [M::mem_pestat] mean and std.dev: (597.74, 1012.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5761) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (95, 169, 953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2669) [M::mem_pestat] mean and std.dev: (422.24, 581.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3527) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925874 reads in 1006.659 CPU sec, 62.310 real sec [M::process] 0 single-end sequences; 1925988 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1246, 752277, 60, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 108, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (124.22, 73.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.11) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 121, 767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2237) [M::mem_pestat] mean and std.dev: (246.37, 425.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2972) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (106, 651, 1323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3757) [M::mem_pestat] mean and std.dev: (675.20, 656.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4974) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925988 reads in 951.368 CPU sec, 43.232 real sec [M::process] read 1925948 sequences (240000156 bp)... [M::process] 0 single-end sequences; 1925948 paired-end sequences [M::process] read 1925908 sequences (240000076 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1265, 752065, 48, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 106, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (120.70, 72.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 96, 1231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3653) [M::mem_pestat] mean and std.dev: (310.95, 550.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4864) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (120, 198, 890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2430) [M::mem_pestat] mean and std.dev: (466.00, 529.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3200) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925948 reads in 983.704 CPU sec, 63.478 real sec [M::process] 0 single-end sequences; 1925908 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1310, 751824, 43, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 110, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (125.39, 73.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 166, 2039) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6063) [M::mem_pestat] mean and std.dev: (1036.73, 1476.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8075) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (132, 218, 845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2271) [M::mem_pestat] mean and std.dev: (423.31, 498.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2984) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925908 reads in 966.326 CPU sec, 42.649 real sec [M::process] read 1925918 sequences (240000120 bp)... [M::process] 0 single-end sequences; 1925918 paired-end sequences [M::process] read 1925850 sequences (240000026 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1201, 752143, 55, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 103, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (121.23, 71.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 83, 2804) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8358) [M::mem_pestat] mean and std.dev: (1350.93, 2033.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11135) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (141, 360, 1118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3072) [M::mem_pestat] mean and std.dev: (683.44, 712.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4049) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925918 reads in 998.721 CPU sec, 63.230 real sec [M::process] 0 single-end sequences; 1925850 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1258, 752100, 57, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 111, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (125.53, 73.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.11) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 196, 1967) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5847) [M::mem_pestat] mean and std.dev: (975.89, 1480.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7787) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (94, 194, 911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2545) [M::mem_pestat] mean and std.dev: (413.86, 510.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3362) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925850 reads in 964.894 CPU sec, 42.416 real sec [M::process] read 1925796 sequences (240000244 bp)... [M::process] 0 single-end sequences; 1925796 paired-end sequences [M::process] read 1925832 sequences (240000222 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1214, 751947, 57, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 111, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (127.07, 74.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 55, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2166) [M::mem_pestat] mean and std.dev: (171.98, 306.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2881) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (108, 199, 1089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3051) [M::mem_pestat] mean and std.dev: (522.73, 712.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4032) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925796 reads in 988.787 CPU sec, 64.853 real sec [M::process] 0 single-end sequences; 1925832 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1266, 751948, 42, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 116, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (127.45, 74.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.24) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (61, 311, 3779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11215) [M::mem_pestat] mean and std.dev: (1654.93, 2010.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14933) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (136, 203, 1029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2815) [M::mem_pestat] mean and std.dev: (519.71, 684.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3708) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925832 reads in 952.867 CPU sec, 41.886 real sec [M::process] read 1925928 sequences (240000244 bp)... [M::process] 0 single-end sequences; 1925928 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1290, 751705, 38, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 117, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (127.00, 72.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.32, 30.20) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (67, 190, 4372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12982) [M::mem_pestat] mean and std.dev: (1637.84, 2346.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17287) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (95, 185, 847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2351) [M::mem_pestat] mean and std.dev: (368.00, 431.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3103) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925928 reads in 967.024 CPU sec, 53.553 real sec [M::process] read 1926024 sequences (240000156 bp)... [M::process] 0 single-end sequences; 1926024 paired-end sequences [M::process] read 1925910 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1272, 752764, 47, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 113, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (126.51, 74.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 100, 1358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3998) [M::mem_pestat] mean and std.dev: (441.34, 858.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5318) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (78, 181, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 753) [M::mem_pestat] mean and std.dev: (163.48, 137.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1926024 reads in 1005.029 CPU sec, 62.879 real sec [M::process] 0 single-end sequences; 1925910 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1251, 752216, 37, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 109, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (123.65, 73.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 108, 1908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5684) [M::mem_pestat] mean and std.dev: (1002.54, 1503.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7572) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (109, 189, 807) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2203) [M::mem_pestat] mean and std.dev: (415.11, 506.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2901) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925910 reads in 965.506 CPU sec, 44.188 real sec [M::process] read 1925796 sequences (240000246 bp)... [M::process] 0 single-end sequences; 1925796 paired-end sequences [M::process] read 1925886 sequences (240000168 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1246, 751696, 60, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 108, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (123.13, 71.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 161, 3026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9028) [M::mem_pestat] mean and std.dev: (1262.80, 1704.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12029) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (118, 195, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (176.29, 130.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925796 reads in 1005.988 CPU sec, 63.837 real sec [M::process] 0 single-end sequences; 1925886 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1210, 751542, 43, 61) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 113, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (128.22, 75.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 63, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1341) [M::mem_pestat] mean and std.dev: (98.18, 199.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1780) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (97, 178, 1044) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2938) [M::mem_pestat] mean and std.dev: (578.28, 759.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3885) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925886 reads in 947.328 CPU sec, 41.810 real sec [M::process] read 1925970 sequences (240000120 bp)... [M::process] 0 single-end sequences; 1925970 paired-end sequences [M::process] read 1925986 sequences (240000188 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1272, 752262, 46, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 116, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (128.62, 73.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (70, 739, 3066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9058) [M::mem_pestat] mean and std.dev: (1805.63, 2143.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12054) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (103, 198, 875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2419) [M::mem_pestat] mean and std.dev: (424.49, 540.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3191) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925970 reads in 1003.698 CPU sec, 63.380 real sec [M::process] 0 single-end sequences; 1925986 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1289, 751448, 42, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 112, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (125.13, 72.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (68, 215, 3587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10625) [M::mem_pestat] mean and std.dev: (1715.83, 2310.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14144) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (105, 268, 945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2625) [M::mem_pestat] mean and std.dev: (449.46, 567.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3465) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925986 reads in 958.293 CPU sec, 42.204 real sec [M::process] read 1925908 sequences (240000222 bp)... [M::process] 0 single-end sequences; 1925908 paired-end sequences [M::process] read 1925998 sequences (240000214 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1210, 751529, 35, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 113, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (126.66, 73.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 162, 1409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4131) [M::mem_pestat] mean and std.dev: (532.19, 791.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5492) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (107, 191, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1466) [M::mem_pestat] mean and std.dev: (283.82, 316.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1919) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925908 reads in 1004.572 CPU sec, 63.900 real sec [M::process] 0 single-end sequences; 1925998 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1268, 752338, 54, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 113, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (125.84, 72.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.11) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 131, 1517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4523) [M::mem_pestat] mean and std.dev: (594.84, 1036.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6026) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (68, 189, 936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2672) [M::mem_pestat] mean and std.dev: (413.00, 576.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3540) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925998 reads in 959.818 CPU sec, 42.731 real sec [M::process] read 1925784 sequences (240000182 bp)... [M::process] 0 single-end sequences; 1925784 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1302, 751553, 48, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 113, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (125.09, 73.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 73, 2982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8902) [M::mem_pestat] mean and std.dev: (1395.27, 1989.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11862) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (163, 228, 682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1720) [M::mem_pestat] mean and std.dev: (415.02, 433.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2239) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925784 reads in 996.204 CPU sec, 65.799 real sec [M::process] read 1925850 sequences (240000090 bp)... [M::process] 0 single-end sequences; 1925850 paired-end sequences [M::process] read 1925936 sequences (240000202 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1226, 751717, 41, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 115, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (126.99, 71.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 400, 3501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10453) [M::mem_pestat] mean and std.dev: (1774.10, 2254.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13929) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 223, 1516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4268) [M::mem_pestat] mean and std.dev: (661.58, 856.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5644) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925850 reads in 997.120 CPU sec, 62.654 real sec [M::process] 0 single-end sequences; 1925936 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1255, 752179, 45, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 110, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (122.19, 69.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 489) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 156, 2826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8426) [M::mem_pestat] mean and std.dev: (1312.58, 1678.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11226) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (113, 181, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 785) [M::mem_pestat] mean and std.dev: (167.22, 120.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1009) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925936 reads in 958.372 CPU sec, 43.175 real sec [M::process] read 1925890 sequences (240000042 bp)... [M::process] 0 single-end sequences; 1925890 paired-end sequences [M::process] read 1925892 sequences (240000186 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1215, 751530, 56, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 112, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (127.03, 73.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 166, 1180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3480) [M::mem_pestat] mean and std.dev: (415.52, 652.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4630) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 368, 1274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3564) [M::mem_pestat] mean and std.dev: (752.59, 797.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4709) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925890 reads in 1002.799 CPU sec, 63.683 real sec [M::process] 0 single-end sequences; 1925892 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1229, 752129, 50, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 116, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (127.79, 73.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.42, 30.10) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 164, 1192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3520) [M::mem_pestat] mean and std.dev: (409.31, 701.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4684) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (79, 201, 1247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3583) [M::mem_pestat] mean and std.dev: (620.25, 776.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4751) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925892 reads in 957.941 CPU sec, 42.271 real sec [M::process] read 1925972 sequences (240000234 bp)... [M::process] 0 single-end sequences; 1925972 paired-end sequences [M::process] read 1925918 sequences (240000246 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1230, 752123, 33, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 112, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (125.23, 72.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 189, 4402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13136) [M::mem_pestat] mean and std.dev: (1869.45, 2319.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17503) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (108, 182, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (138.53, 80.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 868) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925972 reads in 996.949 CPU sec, 63.939 real sec [M::process] 0 single-end sequences; 1925918 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1266, 751402, 52, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 110, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (126.25, 76.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.33, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 157, 2549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7597) [M::mem_pestat] mean and std.dev: (1321.65, 2004.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10121) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 206, 1036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2842) [M::mem_pestat] mean and std.dev: (511.03, 633.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3745) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925918 reads in 958.223 CPU sec, 42.209 real sec [M::process] read 1925970 sequences (240000202 bp)... [M::process] 0 single-end sequences; 1925970 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1276, 750805, 40, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 112, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (126.61, 72.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 135, 1685) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5015) [M::mem_pestat] mean and std.dev: (818.55, 1283.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6680) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (109, 188, 877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2413) [M::mem_pestat] mean and std.dev: (428.34, 532.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3181) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1925932 sequences (240000012 bp)... [M::mem_process_seqs] Processed 1925970 reads in 1002.614 CPU sec, 65.198 real sec [M::process] 0 single-end sequences; 1925932 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1282, 751844, 36, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 108, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (121.43, 69.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 118, 2373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7081) [M::mem_pestat] mean and std.dev: (1014.49, 1612.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9435) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (135, 202, 1012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2766) [M::mem_pestat] mean and std.dev: (518.32, 475.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3643) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925932 reads in 947.162 CPU sec, 41.595 real sec [M::process] read 1925908 sequences (240000222 bp)... [M::process] 0 single-end sequences; 1925908 paired-end sequences [M::process] read 1925982 sequences (240000112 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1249, 751927, 61, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 109, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (122.55, 70.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.10) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 149, 2628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7848) [M::mem_pestat] mean and std.dev: (1194.07, 1825.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10458) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (128, 267, 1098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3038) [M::mem_pestat] mean and std.dev: (648.02, 737.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4008) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925908 reads in 993.263 CPU sec, 63.428 real sec [M::process] 0 single-end sequences; 1925982 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1316, 751961, 51, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 111, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (125.89, 73.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 109, 1999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5927) [M::mem_pestat] mean and std.dev: (779.35, 1406.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7891) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (67, 168, 287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 727) [M::mem_pestat] mean and std.dev: (118.68, 74.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925982 reads in 954.200 CPU sec, 41.964 real sec [M::process] read 1925832 sequences (240000150 bp)... [M::process] 0 single-end sequences; 1925832 paired-end sequences [M::process] read 1925964 sequences (240000098 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1210, 752886, 49, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 114, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (129.89, 75.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.43, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 59, 1341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4003) [M::mem_pestat] mean and std.dev: (398.55, 760.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5334) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 250, 1041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2843) [M::mem_pestat] mean and std.dev: (566.15, 682.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3744) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925832 reads in 991.101 CPU sec, 62.435 real sec [M::process] 0 single-end sequences; 1925964 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1291, 752473, 57, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 111, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (125.67, 72.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 175, 3114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9260) [M::mem_pestat] mean and std.dev: (1488.79, 2051.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12333) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (90, 188, 1008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2844) [M::mem_pestat] mean and std.dev: (469.26, 640.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3762) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925964 reads in 964.660 CPU sec, 43.846 real sec [M::process] read 1925934 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1925934 paired-end sequences [M::process] read 1925946 sequences (240000002 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1233, 750914, 53, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 112, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (125.75, 72.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (48, 147, 1966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5802) [M::mem_pestat] mean and std.dev: (1052.47, 1538.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7720) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (106, 193, 317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (171.85, 115.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 950) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925934 reads in 994.922 CPU sec, 64.674 real sec [M::process] 0 single-end sequences; 1925946 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1245, 752431, 52, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 108, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (122.96, 73.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 186, 2705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8033) [M::mem_pestat] mean and std.dev: (1324.88, 1789.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10697) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (113, 217, 1153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3233) [M::mem_pestat] mean and std.dev: (501.28, 662.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4273) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925946 reads in 962.598 CPU sec, 42.534 real sec [M::process] read 1925926 sequences (240000130 bp)... [M::process] 0 single-end sequences; 1925926 paired-end sequences [M::process] read 1925862 sequences (240000246 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1249, 751768, 58, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 121, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (130.98, 75.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.33, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 52, 582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1710) [M::mem_pestat] mean and std.dev: (175.19, 342.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2274) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (125, 195, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (151.92, 77.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 897) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925926 reads in 996.882 CPU sec, 63.409 real sec [M::process] 0 single-end sequences; 1925862 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1318, 751940, 50, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 112, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (125.82, 73.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 192, 2978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8868) [M::mem_pestat] mean and std.dev: (1499.94, 2232.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11813) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (117, 227, 327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 747) [M::mem_pestat] mean and std.dev: (180.18, 122.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925862 reads in 950.997 CPU sec, 41.828 real sec [M::process] read 1925836 sequences (240000176 bp)... [M::process] 0 single-end sequences; 1925836 paired-end sequences [M::process] read 1925926 sequences (240000086 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1247, 752477, 42, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 103, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (119.26, 70.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.42, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 37, 1745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5209) [M::mem_pestat] mean and std.dev: (792.77, 1555.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7013) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (77, 188, 1176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3374) [M::mem_pestat] mean and std.dev: (599.50, 767.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4473) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925836 reads in 990.557 CPU sec, 64.380 real sec [M::process] 0 single-end sequences; 1925926 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1275, 752433, 55, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 115, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (128.16, 74.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.44, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 160, 2339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6933) [M::mem_pestat] mean and std.dev: (1234.43, 1686.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9230) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (56, 137, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (131.05, 102.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 728) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925926 reads in 959.260 CPU sec, 42.188 real sec [M::process] read 1925838 sequences (240000152 bp)... [M::process] 0 single-end sequences; 1925838 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1286, 752194, 49, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 115, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (128.13, 73.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 38, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 554) [M::mem_pestat] mean and std.dev: (62.87, 85.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 733) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (108, 164, 997) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2775) [M::mem_pestat] mean and std.dev: (356.14, 483.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3664) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925838 reads in 984.807 CPU sec, 61.415 real sec [M::process] read 1925898 sequences (240000162 bp)... [M::process] 0 single-end sequences; 1925898 paired-end sequences [M::process] read 1925862 sequences (240000176 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1272, 752404, 46, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 111, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (123.64, 72.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 342, 4363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13023) [M::mem_pestat] mean and std.dev: (1847.04, 2326.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17353) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (83, 234, 744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2066) [M::mem_pestat] mean and std.dev: (350.85, 429.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2727) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925898 reads in 1005.684 CPU sec, 62.933 real sec [M::process] 0 single-end sequences; 1925862 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1236, 752517, 52, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 112, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (125.88, 74.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 91, 1915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5715) [M::mem_pestat] mean and std.dev: (941.10, 1530.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7615) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (87, 168, 810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2256) [M::mem_pestat] mean and std.dev: (351.02, 485.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2979) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925862 reads in 966.342 CPU sec, 44.199 real sec [M::process] read 1926020 sequences (240000202 bp)... [M::process] 0 single-end sequences; 1926020 paired-end sequences [M::process] read 1925890 sequences (240000160 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1277, 752519, 52, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 106, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (124.34, 74.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 138, 3007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8927) [M::mem_pestat] mean and std.dev: (1425.85, 2120.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11887) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (96, 196, 812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2244) [M::mem_pestat] mean and std.dev: (316.54, 411.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2960) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1926020 reads in 985.693 CPU sec, 64.514 real sec [M::process] 0 single-end sequences; 1925890 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1252, 751127, 56, 34) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 112, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (127.78, 74.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.10) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 407, 4371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13035) [M::mem_pestat] mean and std.dev: (1838.77, 2260.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17367) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (90, 154, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (128.04, 69.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925890 reads in 960.041 CPU sec, 42.368 real sec [M::process] read 1925988 sequences (240000046 bp)... [M::process] 0 single-end sequences; 1925988 paired-end sequences [M::process] read 1925922 sequences (240000194 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1229, 751934, 38, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 109, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (125.39, 73.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.30, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 140, 1790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5314) [M::mem_pestat] mean and std.dev: (1142.73, 1718.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8017) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (106, 195, 368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (200.75, 173.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1154) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925988 reads in 979.137 CPU sec, 64.091 real sec [M::process] 0 single-end sequences; 1925922 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1252, 751784, 60, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 111, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (124.77, 71.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 143, 1112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3280) [M::mem_pestat] mean and std.dev: (415.72, 704.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4364) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (110, 195, 1182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3326) [M::mem_pestat] mean and std.dev: (563.55, 758.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4398) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925922 reads in 955.736 CPU sec, 42.036 real sec [M::process] read 1925898 sequences (240000098 bp)... [M::process] 0 single-end sequences; 1925898 paired-end sequences [M::process] read 1925900 sequences (240000182 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1297, 752339, 59, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 110, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (126.10, 73.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 188, 2688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7972) [M::mem_pestat] mean and std.dev: (1164.90, 1615.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10614) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (88, 176, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (146.65, 97.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925898 reads in 997.434 CPU sec, 63.727 real sec [M::process] 0 single-end sequences; 1925900 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1303, 751416, 44, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 112, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (125.97, 72.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.33, 30.21) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 169, 3616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10776) [M::mem_pestat] mean and std.dev: (1719.75, 2313.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14356) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (113, 175, 811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2207) [M::mem_pestat] mean and std.dev: (276.34, 343.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2905) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925900 reads in 954.583 CPU sec, 42.047 real sec [M::process] read 1925924 sequences (240000116 bp)... [M::process] 0 single-end sequences; 1925924 paired-end sequences [M::process] read 1925938 sequences (240000092 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1242, 752952, 40, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 110, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (124.61, 73.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 102, 4103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12259) [M::mem_pestat] mean and std.dev: (1793.85, 2495.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16337) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 595, 1091) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2995) [M::mem_pestat] mean and std.dev: (672.34, 632.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3947) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925924 reads in 992.484 CPU sec, 64.484 real sec [M::process] 0 single-end sequences; 1925938 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1206, 751474, 61, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 114, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (125.99, 72.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.30, 30.28) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 161, 4120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12310) [M::mem_pestat] mean and std.dev: (1634.33, 2089.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16405) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (86, 196, 577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1559) [M::mem_pestat] mean and std.dev: (273.88, 297.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2050) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925938 reads in 960.501 CPU sec, 42.171 real sec [M::process] read 1925904 sequences (240000048 bp)... [M::process] 0 single-end sequences; 1925904 paired-end sequences [M::process] read 1925906 sequences (240000160 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1264, 752437, 49, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 115, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (127.88, 72.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.47, 30.07) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 147, 2232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6672) [M::mem_pestat] mean and std.dev: (1131.33, 1617.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8892) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (63, 172, 1024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2946) [M::mem_pestat] mean and std.dev: (496.78, 652.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3907) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925904 reads in 980.144 CPU sec, 62.254 real sec [M::process] 0 single-end sequences; 1925906 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1216, 750671, 46, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 112, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (124.87, 71.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.30, 30.26) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 131, 1972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5882) [M::mem_pestat] mean and std.dev: (955.47, 1499.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7837) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 240, 878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2376) [M::mem_pestat] mean and std.dev: (404.45, 450.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3125) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925906 reads in 970.658 CPU sec, 44.262 real sec [M::process] read 1925880 sequences (240000158 bp)... [M::process] 0 single-end sequences; 1925880 paired-end sequences [M::process] read 1925952 sequences (240000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1184, 752057, 53, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 116, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (125.97, 72.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 415, 4376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13046) [M::mem_pestat] mean and std.dev: (1705.25, 2117.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17381) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (103, 160, 588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1558) [M::mem_pestat] mean and std.dev: (291.02, 329.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2043) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925880 reads in 998.922 CPU sec, 64.066 real sec [M::process] 0 single-end sequences; 1925952 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1227, 751706, 51, 31) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 110, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (125.55, 73.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 159, 906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2636) [M::mem_pestat] mean and std.dev: (316.76, 567.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3501) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (159, 207, 1576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4410) [M::mem_pestat] mean and std.dev: (705.67, 884.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5827) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925952 reads in 954.809 CPU sec, 42.186 real sec [M::process] read 1925926 sequences (240000202 bp)... [M::process] 0 single-end sequences; 1925926 paired-end sequences [M::process] read 1925920 sequences (240000158 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1243, 752152, 49, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 107, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (123.97, 73.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.10) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 232, 4392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13110) [M::mem_pestat] mean and std.dev: (2138.22, 2769.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17469) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (67, 186, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (143.59, 93.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 843) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925926 reads in 997.335 CPU sec, 63.502 real sec [M::process] 0 single-end sequences; 1925920 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1302, 751855, 66, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 110, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (124.66, 73.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 156, 1654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4904) [M::mem_pestat] mean and std.dev: (982.98, 1509.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7020) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (140, 210, 856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2288) [M::mem_pestat] mean and std.dev: (463.74, 533.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3004) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925920 reads in 956.814 CPU sec, 42.511 real sec [M::process] read 1925908 sequences (240000212 bp)... [M::process] 0 single-end sequences; 1925908 paired-end sequences [M::process] read 1925848 sequences (240000052 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1336, 752242, 37, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 111, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (126.28, 74.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (58, 169, 4380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13024) [M::mem_pestat] mean and std.dev: (1602.11, 2344.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17346) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (84, 237, 962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2718) [M::mem_pestat] mean and std.dev: (452.26, 553.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3596) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925908 reads in 999.001 CPU sec, 63.546 real sec [M::process] 0 single-end sequences; 1925848 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1249, 752305, 46, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 113, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (125.73, 71.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.33, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 124, 1885) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5607) [M::mem_pestat] mean and std.dev: (966.53, 1537.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7468) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (87, 193, 795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2211) [M::mem_pestat] mean and std.dev: (402.88, 499.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2919) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925848 reads in 955.175 CPU sec, 43.332 real sec [M::process] read 1925876 sequences (240000234 bp)... [M::process] 0 single-end sequences; 1925876 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1252, 751654, 47, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 113, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (125.25, 71.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.10) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 172, 1777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5291) [M::mem_pestat] mean and std.dev: (884.09, 1452.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7048) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (150, 260, 896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2388) [M::mem_pestat] mean and std.dev: (452.51, 490.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3134) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925876 reads in 984.308 CPU sec, 56.863 real sec [M::process] read 1925890 sequences (240000162 bp)... [M::process] 0 single-end sequences; 1925890 paired-end sequences [M::process] read 1925956 sequences (240000084 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1258, 752793, 48, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 110, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (125.33, 75.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 146, 2421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7219) [M::mem_pestat] mean and std.dev: (1167.94, 1733.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9618) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (127, 199, 1592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4522) [M::mem_pestat] mean and std.dev: (629.07, 803.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5987) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925890 reads in 985.288 CPU sec, 62.681 real sec [M::process] 0 single-end sequences; 1925956 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1207, 752058, 49, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 102, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (120.64, 72.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.23) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 140, 1747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5189) [M::mem_pestat] mean and std.dev: (983.50, 1471.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6910) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (88, 187, 932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2620) [M::mem_pestat] mean and std.dev: (397.11, 456.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3464) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925956 reads in 960.689 CPU sec, 43.603 real sec [M::process] read 1925918 sequences (240000214 bp)... [M::process] 0 single-end sequences; 1925918 paired-end sequences [M::process] read 1925846 sequences (240000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1224, 751211, 56, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 113, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (125.29, 71.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.11) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 150, 1536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4554) [M::mem_pestat] mean and std.dev: (880.17, 1432.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6609) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (109, 739, 1302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3688) [M::mem_pestat] mean and std.dev: (736.97, 732.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4881) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925918 reads in 1002.998 CPU sec, 63.638 real sec [M::process] 0 single-end sequences; 1925846 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1249, 752617, 46, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 115, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (128.11, 74.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 133, 1939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5795) [M::mem_pestat] mean and std.dev: (1065.28, 1652.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7723) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (113, 217, 1246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3512) [M::mem_pestat] mean and std.dev: (720.71, 953.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4645) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925846 reads in 952.531 CPU sec, 42.022 real sec [M::process] read 1925844 sequences (240000020 bp)... [M::process] 0 single-end sequences; 1925844 paired-end sequences [M::process] read 1925894 sequences (240000248 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1262, 751934, 40, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 108, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (124.01, 73.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 177, 2042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6074) [M::mem_pestat] mean and std.dev: (970.18, 1537.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8090) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (168, 309, 1150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3114) [M::mem_pestat] mean and std.dev: (637.56, 633.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4096) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925844 reads in 987.131 CPU sec, 63.651 real sec [M::process] 0 single-end sequences; 1925894 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1257, 751617, 51, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 114, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (127.84, 75.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 116, 1808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5340) [M::mem_pestat] mean and std.dev: (865.62, 1365.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7106) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (118, 210, 1027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2845) [M::mem_pestat] mean and std.dev: (526.20, 597.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3754) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925894 reads in 946.111 CPU sec, 41.619 real sec [M::process] read 1926002 sequences (240000090 bp)... [M::process] 0 single-end sequences; 1926002 paired-end sequences [M::process] read 1925916 sequences (240000134 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1185, 751881, 38, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 108, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (123.77, 71.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.11) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 198, 2279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6763) [M::mem_pestat] mean and std.dev: (1239.89, 1772.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9005) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 329, 1294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3584) [M::mem_pestat] mean and std.dev: (789.85, 822.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4729) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1926002 reads in 992.320 CPU sec, 63.466 real sec [M::process] 0 single-end sequences; 1925916 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1283, 752517, 63, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 118, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (129.21, 73.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 234, 3098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9194) [M::mem_pestat] mean and std.dev: (1670.05, 2401.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12242) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 259, 996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2730) [M::mem_pestat] mean and std.dev: (459.59, 450.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3597) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925916 reads in 952.529 CPU sec, 41.838 real sec [M::process] read 1925854 sequences (240000220 bp)... [M::process] 0 single-end sequences; 1925854 paired-end sequences [M::process] read 1926022 sequences (240000128 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1196, 751634, 31, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 112, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (126.57, 74.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.21) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (49, 162, 2556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7570) [M::mem_pestat] mean and std.dev: (1124.45, 1715.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10077) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (101, 202, 392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (175.36, 122.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1265) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925854 reads in 1003.476 CPU sec, 65.014 real sec [M::process] 0 single-end sequences; 1926022 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1264, 751560, 42, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 110, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (122.17, 70.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 176, 3692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11036) [M::mem_pestat] mean and std.dev: (1511.02, 1863.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14708) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (130, 238, 959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2617) [M::mem_pestat] mean and std.dev: (493.74, 631.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3446) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1926022 reads in 943.166 CPU sec, 41.459 real sec [M::process] read 1925978 sequences (240000042 bp)... [M::process] 0 single-end sequences; 1925978 paired-end sequences [M::process] read 1925906 sequences (240000166 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1229, 753196, 57, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 115, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (127.04, 73.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.33, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 169, 2664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7954) [M::mem_pestat] mean and std.dev: (1227.71, 1732.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10599) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (123, 189, 887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2415) [M::mem_pestat] mean and std.dev: (401.00, 504.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3179) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925978 reads in 978.400 CPU sec, 62.490 real sec [M::process] 0 single-end sequences; 1925906 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1248, 751595, 44, 57) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 111, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (122.56, 69.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.09) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 152, 4388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13074) [M::mem_pestat] mean and std.dev: (1850.77, 2059.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17417) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (151, 242, 968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2602) [M::mem_pestat] mean and std.dev: (494.18, 458.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3419) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925906 reads in 964.876 CPU sec, 44.108 real sec [M::process] read 1925886 sequences (240000138 bp)... [M::process] 0 single-end sequences; 1925886 paired-end sequences [M::process] read 1925916 sequences (240000076 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1303, 751352, 50, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 111, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (125.11, 73.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 136, 3058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9142) [M::mem_pestat] mean and std.dev: (1192.88, 1692.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12184) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (120, 197, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (166.29, 106.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925886 reads in 982.504 CPU sec, 62.365 real sec [M::process] 0 single-end sequences; 1925916 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1264, 751852, 47, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 114, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (126.46, 72.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.32, 30.21) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 167, 3086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9200) [M::mem_pestat] mean and std.dev: (1465.91, 2048.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12257) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (93, 168, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 567) [M::mem_pestat] mean and std.dev: (132.55, 69.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925916 reads in 958.385 CPU sec, 42.365 real sec [M::process] read 1925944 sequences (240000002 bp)... [M::process] 0 single-end sequences; 1925944 paired-end sequences [M::process] read 1925974 sequences (240000182 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1207, 751668, 50, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 112, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (123.86, 71.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 156, 2101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6249) [M::mem_pestat] mean and std.dev: (1261.84, 1699.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8323) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 225, 839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2229) [M::mem_pestat] mean and std.dev: (452.05, 494.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2924) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925944 reads in 995.262 CPU sec, 62.689 real sec [M::process] 0 single-end sequences; 1925974 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1285, 751944, 49, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 115, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (128.11, 72.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 91, 1697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5051) [M::mem_pestat] mean and std.dev: (946.52, 1524.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7044) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (90, 164, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 732) [M::mem_pestat] mean and std.dev: (159.75, 113.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 946) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925974 reads in 947.828 CPU sec, 41.784 real sec [M::process] read 1925868 sequences (240000188 bp)... [M::process] 0 single-end sequences; 1925868 paired-end sequences [M::process] read 1925876 sequences (240000130 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1161, 752023, 53, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 114, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (129.18, 72.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 170, 2644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7860) [M::mem_pestat] mean and std.dev: (1082.04, 1636.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10468) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (101, 208, 688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1862) [M::mem_pestat] mean and std.dev: (390.57, 504.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2449) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925868 reads in 990.462 CPU sec, 62.703 real sec [M::process] 0 single-end sequences; 1925876 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1279, 751718, 49, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 109, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (126.80, 74.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 147, 1956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5808) [M::mem_pestat] mean and std.dev: (910.81, 1402.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7734) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (105, 182, 747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2031) [M::mem_pestat] mean and std.dev: (367.42, 425.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2673) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925876 reads in 942.786 CPU sec, 41.811 real sec [M::process] read 1925902 sequences (240000160 bp)... [M::process] 0 single-end sequences; 1925902 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1291, 752456, 43, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 106, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (124.44, 72.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 126, 1049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3069) [M::mem_pestat] mean and std.dev: (385.14, 748.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4079) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 202, 760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2018) [M::mem_pestat] mean and std.dev: (401.18, 432.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2647) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925902 reads in 957.116 CPU sec, 52.494 real sec [M::process] read 1925882 sequences (240000172 bp)... [M::process] 0 single-end sequences; 1925882 paired-end sequences [M::process] read 1925932 sequences (240000190 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1256, 751747, 54, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 109, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (124.65, 72.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 141, 3146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9360) [M::mem_pestat] mean and std.dev: (1451.19, 2229.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12467) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (118, 185, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (162.64, 115.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925882 reads in 995.707 CPU sec, 61.828 real sec [M::process] 0 single-end sequences; 1925932 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1238, 751788, 53, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 106, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (124.50, 73.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 134, 4370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13040) [M::mem_pestat] mean and std.dev: (1612.40, 2076.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17375) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (130, 239, 962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2626) [M::mem_pestat] mean and std.dev: (497.96, 557.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3458) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925932 reads in 957.329 CPU sec, 43.933 real sec [M::process] read 1925916 sequences (240000110 bp)... [M::process] 0 single-end sequences; 1925916 paired-end sequences [M::process] read 1925990 sequences (240000134 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1232, 752222, 48, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 105, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (121.79, 71.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.28, 30.22) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 133, 1047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3077) [M::mem_pestat] mean and std.dev: (368.46, 715.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4092) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 241, 1662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4672) [M::mem_pestat] mean and std.dev: (689.71, 838.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6177) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925916 reads in 992.855 CPU sec, 62.893 real sec [M::process] 0 single-end sequences; 1925990 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1256, 751556, 47, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 111, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (125.40, 73.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 60, 1133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3359) [M::mem_pestat] mean and std.dev: (390.93, 804.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4472) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (87, 173, 810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2256) [M::mem_pestat] mean and std.dev: (399.53, 470.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2979) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925990 reads in 939.857 CPU sec, 41.421 real sec [M::process] read 1925936 sequences (240000194 bp)... [M::process] 0 single-end sequences; 1925936 paired-end sequences [M::process] read 1925852 sequences (240000010 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1233, 751547, 52, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 108, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (122.85, 72.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.11) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 76, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1800) [M::mem_pestat] mean and std.dev: (137.60, 232.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2392) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (91, 173, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2026) [M::mem_pestat] mean and std.dev: (368.33, 478.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2671) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925936 reads in 974.040 CPU sec, 62.803 real sec [M::process] 0 single-end sequences; 1925852 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1255, 751892, 52, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 107, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (124.27, 72.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.42, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 139, 1759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5221) [M::mem_pestat] mean and std.dev: (943.66, 1423.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6952) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (63, 193, 875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2499) [M::mem_pestat] mean and std.dev: (286.50, 479.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3311) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925852 reads in 956.873 CPU sec, 42.120 real sec [M::process] read 1925922 sequences (240000220 bp)... [M::process] 0 single-end sequences; 1925922 paired-end sequences [M::process] read 1926008 sequences (240000186 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1193, 752169, 53, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 118, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (129.51, 74.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.07) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 193, 4370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13034) [M::mem_pestat] mean and std.dev: (1582.06, 2191.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17366) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (83, 154, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 935) [M::mem_pestat] mean and std.dev: (198.33, 224.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1219) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925922 reads in 983.441 CPU sec, 62.469 real sec [M::process] 0 single-end sequences; 1926008 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1274, 751984, 44, 30) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 115, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (129.47, 74.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 128, 1093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3239) [M::mem_pestat] mean and std.dev: (324.41, 633.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4312) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (154, 527, 1332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3688) [M::mem_pestat] mean and std.dev: (621.64, 576.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4866) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1926008 reads in 940.148 CPU sec, 41.360 real sec [M::process] read 1925886 sequences (240000226 bp)... [M::process] 0 single-end sequences; 1925886 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1258, 751682, 43, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 110, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (124.59, 73.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.33, 30.20) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 180, 2840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8462) [M::mem_pestat] mean and std.dev: (1332.00, 1854.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11273) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (97, 174, 492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1282) [M::mem_pestat] mean and std.dev: (219.90, 243.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1677) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925886 reads in 979.968 CPU sec, 62.581 real sec [M::process] read 1925916 sequences (240000124 bp)... [M::process] 0 single-end sequences; 1925916 paired-end sequences [M::process] read 1925920 sequences (240000128 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1278, 751683, 44, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 107, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (121.92, 71.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.11) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 162, 2667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7955) [M::mem_pestat] mean and std.dev: (1163.74, 1705.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10599) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (104, 205, 1016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2840) [M::mem_pestat] mean and std.dev: (433.90, 592.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3752) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925916 reads in 983.779 CPU sec, 61.923 real sec [M::process] 0 single-end sequences; 1925920 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1255, 752354, 47, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 112, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (125.73, 73.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.10) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 167, 2322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6884) [M::mem_pestat] mean and std.dev: (1009.25, 1545.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9165) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 211, 976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2662) [M::mem_pestat] mean and std.dev: (486.72, 608.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3505) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925920 reads in 955.807 CPU sec, 43.546 real sec [M::process] read 1925936 sequences (240000222 bp)... [M::process] 0 single-end sequences; 1925936 paired-end sequences [M::process] read 1925884 sequences (240000070 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1283, 751454, 47, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 106, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 381) [M::mem_pestat] mean and std.dev: (121.15, 72.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 95, 2241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6677) [M::mem_pestat] mean and std.dev: (1058.16, 1600.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8895) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (101, 192, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (162.73, 101.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 817) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925936 reads in 985.960 CPU sec, 62.599 real sec [M::process] 0 single-end sequences; 1925884 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1244, 751677, 46, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 112, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (126.29, 75.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 326, 3034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9068) [M::mem_pestat] mean and std.dev: (1525.67, 2172.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12085) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (81, 186, 1432) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4134) [M::mem_pestat] mean and std.dev: (657.13, 999.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5485) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925884 reads in 949.140 CPU sec, 41.865 real sec [M::process] read 1925864 sequences (240000064 bp)... [M::process] 0 single-end sequences; 1925864 paired-end sequences [M::process] read 1925964 sequences (240000076 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1229, 751846, 57, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 109, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (124.80, 74.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 78, 2707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8093) [M::mem_pestat] mean and std.dev: (1145.93, 1764.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10786) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (98, 169, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 791) [M::mem_pestat] mean and std.dev: (196.54, 175.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1022) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925864 reads in 977.693 CPU sec, 62.572 real sec [M::process] 0 single-end sequences; 1925964 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1244, 752388, 59, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 114, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (127.86, 73.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.33, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 89, 1897) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5637) [M::mem_pestat] mean and std.dev: (921.60, 1537.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7507) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (62, 206, 753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2135) [M::mem_pestat] mean and std.dev: (403.62, 473.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925964 reads in 940.136 CPU sec, 41.380 real sec [M::process] read 1925874 sequences (240000002 bp)... [M::process] 0 single-end sequences; 1925874 paired-end sequences [M::process] read 1925962 sequences (240000038 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1254, 751905, 49, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 114, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (125.73, 73.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.44, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 178, 2405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7131) [M::mem_pestat] mean and std.dev: (1094.79, 1596.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9494) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (102, 199, 1031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2889) [M::mem_pestat] mean and std.dev: (486.46, 639.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3818) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925874 reads in 983.361 CPU sec, 63.115 real sec [M::process] 0 single-end sequences; 1925962 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1231, 752024, 53, 36) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 109, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 420) [M::mem_pestat] mean and std.dev: (124.72, 73.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.31, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 102, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1708) [M::mem_pestat] mean and std.dev: (135.40, 166.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2263) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (81, 174, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (141.37, 82.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925962 reads in 948.000 CPU sec, 41.777 real sec [M::process] read 1925838 sequences (240000090 bp)... [M::process] 0 single-end sequences; 1925838 paired-end sequences [M::process] read 1925894 sequences (240000086 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1217, 752112, 48, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 112, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (127.17, 73.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 163, 4374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13050) [M::mem_pestat] mean and std.dev: (1803.12, 2665.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17388) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (119, 169, 741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1985) [M::mem_pestat] mean and std.dev: (337.61, 416.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2607) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925838 reads in 986.206 CPU sec, 63.452 real sec [M::process] 0 single-end sequences; 1925894 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1282, 752153, 56, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 107, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (123.30, 73.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (56, 369, 3810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11318) [M::mem_pestat] mean and std.dev: (1892.04, 2343.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15072) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (106, 205, 856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2356) [M::mem_pestat] mean and std.dev: (379.68, 446.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3106) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925894 reads in 950.802 CPU sec, 41.826 real sec [M::process] read 1925898 sequences (240000160 bp)... [M::process] 0 single-end sequences; 1925898 paired-end sequences [M::process] read 1925882 sequences (240000104 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1254, 751583, 53, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 112, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (127.41, 72.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 80, 1259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3737) [M::mem_pestat] mean and std.dev: (460.30, 866.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4976) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (121, 222, 929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2545) [M::mem_pestat] mean and std.dev: (445.03, 518.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3353) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925898 reads in 988.098 CPU sec, 62.565 real sec [M::process] 0 single-end sequences; 1925882 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1244, 751730, 57, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 112, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (124.72, 71.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 94, 4383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13107) [M::mem_pestat] mean and std.dev: (1558.89, 2269.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17469) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (124, 218, 783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2101) [M::mem_pestat] mean and std.dev: (408.16, 524.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2760) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925882 reads in 949.245 CPU sec, 43.418 real sec [M::process] read 1925982 sequences (240000088 bp)... [M::process] 0 single-end sequences; 1925982 paired-end sequences [M::process] read 1925886 sequences (240000036 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1193, 752603, 41, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 113, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (126.17, 73.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 192, 4389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13117) [M::mem_pestat] mean and std.dev: (2014.83, 2543.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17481) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (144, 205, 769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2019) [M::mem_pestat] mean and std.dev: (331.15, 421.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2644) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925982 reads in 995.422 CPU sec, 63.864 real sec [M::process] 0 single-end sequences; 1925886 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1237, 751988, 57, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 106, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (123.37, 72.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 260, 3249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9673) [M::mem_pestat] mean and std.dev: (1650.47, 2256.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12885) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (122, 192, 786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2114) [M::mem_pestat] mean and std.dev: (315.05, 337.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2778) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925886 reads in 950.483 CPU sec, 41.931 real sec [M::process] read 1925994 sequences (240000088 bp)... [M::process] 0 single-end sequences; 1925994 paired-end sequences [M::process] read 1925858 sequences (240000144 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1300, 751066, 43, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 112, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (125.20, 73.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 97, 3422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10232) [M::mem_pestat] mean and std.dev: (1471.30, 2160.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13637) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (123, 256, 1051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2907) [M::mem_pestat] mean and std.dev: (513.32, 543.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3835) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925994 reads in 999.290 CPU sec, 63.533 real sec [M::process] 0 single-end sequences; 1925858 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1244, 752880, 41, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 114, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (127.50, 73.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 127, 3047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9067) [M::mem_pestat] mean and std.dev: (1306.95, 1685.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12077) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 218, 781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2065) [M::mem_pestat] mean and std.dev: (397.49, 403.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2707) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925858 reads in 953.555 CPU sec, 42.178 real sec [M::process] read 1925882 sequences (240000142 bp)... [M::process] 0 single-end sequences; 1925882 paired-end sequences [M::process] read 1926052 sequences (240000096 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1320, 752166, 46, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 108, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (123.03, 72.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.30, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 205, 2701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8023) [M::mem_pestat] mean and std.dev: (1057.19, 1557.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10684) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 191, 833) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2241) [M::mem_pestat] mean and std.dev: (388.29, 424.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2945) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925882 reads in 996.159 CPU sec, 64.320 real sec [M::process] 0 single-end sequences; 1926052 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1243, 753010, 51, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 116, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (129.01, 74.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.20) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 146, 1397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4145) [M::mem_pestat] mean and std.dev: (448.12, 863.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5519) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (105, 245, 1185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3345) [M::mem_pestat] mean and std.dev: (513.05, 761.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4425) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1926052 reads in 952.117 CPU sec, 41.857 real sec [M::process] read 1925882 sequences (240000140 bp)... [M::process] 0 single-end sequences; 1925882 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1181, 752183, 46, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 106, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (121.89, 70.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 135, 2842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8440) [M::mem_pestat] mean and std.dev: (1247.93, 1960.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11239) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (146, 213, 1175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3233) [M::mem_pestat] mean and std.dev: (540.40, 656.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4262) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925882 reads in 985.633 CPU sec, 61.356 real sec [M::process] read 1925836 sequences (240000226 bp)... [M::process] 0 single-end sequences; 1925836 paired-end sequences [M::process] read 1925914 sequences (240000030 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1238, 751672, 41, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 105, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (120.47, 71.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 151, 2695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8019) [M::mem_pestat] mean and std.dev: (1133.22, 1721.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10681) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (145, 256, 903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2419) [M::mem_pestat] mean and std.dev: (431.08, 447.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3177) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925836 reads in 986.783 CPU sec, 62.446 real sec [M::process] 0 single-end sequences; 1925914 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1246, 751200, 50, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 115, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (126.75, 74.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 74, 1812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5404) [M::mem_pestat] mean and std.dev: (1022.33, 1619.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7501) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (87, 229, 549) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1473) [M::mem_pestat] mean and std.dev: (308.02, 334.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1935) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925914 reads in 966.816 CPU sec, 43.983 real sec [M::process] read 1925852 sequences (240000176 bp)... [M::process] 0 single-end sequences; 1925852 paired-end sequences [M::process] read 1925920 sequences (240000182 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1275, 752399, 64, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 113, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (127.40, 73.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 552, 3648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10896) [M::mem_pestat] mean and std.dev: (1747.66, 2209.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14520) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (84, 171, 299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (156.97, 128.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 944) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925852 reads in 976.185 CPU sec, 63.674 real sec [M::process] 0 single-end sequences; 1925920 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1239, 752081, 51, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 109, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (124.54, 71.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 185, 1946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5770) [M::mem_pestat] mean and std.dev: (1271.86, 1604.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7689) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (102, 161, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 576) [M::mem_pestat] mean and std.dev: (142.41, 85.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 734) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925920 reads in 951.642 CPU sec, 42.011 real sec [M::process] read 1925908 sequences (240000014 bp)... [M::process] 0 single-end sequences; 1925908 paired-end sequences [M::process] read 1925982 sequences (240000198 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1219, 751850, 48, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 113, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (127.67, 75.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 156, 1836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5462) [M::mem_pestat] mean and std.dev: (876.96, 1449.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7275) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (107, 222, 769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2093) [M::mem_pestat] mean and std.dev: (446.90, 511.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2755) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925908 reads in 982.257 CPU sec, 63.867 real sec [M::process] 0 single-end sequences; 1925982 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1288, 751492, 50, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 113, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (127.31, 73.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 406, 2449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7285) [M::mem_pestat] mean and std.dev: (1029.91, 1381.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9703) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (74, 155, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 794) [M::mem_pestat] mean and std.dev: (147.05, 96.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1034) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925982 reads in 953.104 CPU sec, 41.977 real sec [M::process] read 1925932 sequences (240000060 bp)... [M::process] 0 single-end sequences; 1925932 paired-end sequences [M::process] read 1925902 sequences (240000146 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1227, 751741, 57, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 116, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (128.27, 73.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 96, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7779) [M::mem_pestat] mean and std.dev: (1118.71, 1739.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10366) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (181, 234, 965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2533) [M::mem_pestat] mean and std.dev: (433.68, 466.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925932 reads in 983.873 CPU sec, 64.618 real sec [M::process] 0 single-end sequences; 1925902 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1290, 751827, 54, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 113, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (126.13, 71.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.48, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 128, 1744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5174) [M::mem_pestat] mean and std.dev: (1073.42, 1684.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7811) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (97, 181, 1149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3253) [M::mem_pestat] mean and std.dev: (603.32, 798.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4305) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925902 reads in 949.805 CPU sec, 41.749 real sec [M::process] read 1925864 sequences (240000042 bp)... [M::process] 0 single-end sequences; 1925864 paired-end sequences [M::process] read 1925944 sequences (240000048 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1227, 751792, 57, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 108, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (125.36, 74.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.22) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 152, 2557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7609) [M::mem_pestat] mean and std.dev: (1212.16, 1732.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10135) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (90, 168, 529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1407) [M::mem_pestat] mean and std.dev: (292.78, 354.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1846) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925864 reads in 995.148 CPU sec, 64.489 real sec [M::process] 0 single-end sequences; 1925944 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1308, 752033, 56, 53) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 112, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (125.24, 72.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 528) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.20) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 95, 2625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7839) [M::mem_pestat] mean and std.dev: (1275.44, 1922.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10446) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (110, 246, 1381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3923) [M::mem_pestat] mean and std.dev: (714.29, 858.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5194) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925944 reads in 957.428 CPU sec, 42.264 real sec [M::process] read 1925926 sequences (240000214 bp)... [M::process] 0 single-end sequences; 1925926 paired-end sequences [M::process] read 1925970 sequences (240000126 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1157, 752688, 53, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 110, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (124.64, 73.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 167, 2560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7618) [M::mem_pestat] mean and std.dev: (1082.45, 1579.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10147) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (112, 218, 1033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2875) [M::mem_pestat] mean and std.dev: (552.61, 596.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3796) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925926 reads in 997.375 CPU sec, 63.012 real sec [M::process] 0 single-end sequences; 1925970 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1247, 752435, 43, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 119, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (128.69, 72.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (73, 169, 848) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2398) [M::mem_pestat] mean and std.dev: (341.74, 474.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3173) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (97, 156, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 694) [M::mem_pestat] mean and std.dev: (134.38, 79.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925970 reads in 957.005 CPU sec, 43.542 real sec [M::process] read 1925994 sequences (240000134 bp)... [M::process] 0 single-end sequences; 1925994 paired-end sequences [M::process] read 1925938 sequences (240000140 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1195, 751858, 60, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 108, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (122.74, 72.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 167, 3642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10848) [M::mem_pestat] mean and std.dev: (1788.42, 2693.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14451) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (98, 226, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1652) [M::mem_pestat] mean and std.dev: (310.17, 340.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2170) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925994 reads in 985.835 CPU sec, 63.226 real sec [M::process] 0 single-end sequences; 1925938 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1279, 751319, 52, 35) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 107, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (122.22, 71.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 182, 3309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9859) [M::mem_pestat] mean and std.dev: (1610.87, 2024.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13134) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (125, 222, 832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2246) [M::mem_pestat] mean and std.dev: (450.67, 538.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2953) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925938 reads in 953.231 CPU sec, 42.108 real sec [M::process] read 1925888 sequences (240000048 bp)... [M::process] 0 single-end sequences; 1925888 paired-end sequences [M::process] read 1925888 sequences (240000190 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1236, 751362, 52, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 115, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (125.76, 73.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.32, 30.21) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 134, 1757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5225) [M::mem_pestat] mean and std.dev: (970.38, 1541.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7134) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (217, 323, 1330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3556) [M::mem_pestat] mean and std.dev: (622.00, 603.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4669) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925888 reads in 999.980 CPU sec, 63.034 real sec [M::process] 0 single-end sequences; 1925888 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1271, 752211, 36, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 115, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (128.83, 72.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 158, 4401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13167) [M::mem_pestat] mean and std.dev: (1869.00, 2710.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17550) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (78, 140, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1542) [M::mem_pestat] mean and std.dev: (312.83, 384.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2030) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925888 reads in 955.150 CPU sec, 42.091 real sec [M::process] read 1925968 sequences (240000044 bp)... [M::process] 0 single-end sequences; 1925968 paired-end sequences [M::process] read 1925890 sequences (240000020 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1226, 752102, 38, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 111, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (123.73, 72.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 109, 585) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1729) [M::mem_pestat] mean and std.dev: (173.97, 256.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2301) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (161, 232, 1142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3104) [M::mem_pestat] mean and std.dev: (475.76, 588.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4085) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925968 reads in 998.794 CPU sec, 63.258 real sec [M::process] 0 single-end sequences; 1925890 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1309, 752226, 51, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 112, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (125.48, 72.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.43, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 142, 1545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4585) [M::mem_pestat] mean and std.dev: (623.37, 1272.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6105) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (94, 212, 1027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2893) [M::mem_pestat] mean and std.dev: (563.98, 643.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3826) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925890 reads in 956.727 CPU sec, 42.103 real sec [M::process] read 1925934 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1925934 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1256, 752065, 48, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 111, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (124.99, 72.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.12) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 81, 1938) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5760) [M::mem_pestat] mean and std.dev: (875.96, 1503.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7671) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (119, 192, 991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2735) [M::mem_pestat] mean and std.dev: (435.83, 547.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3607) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925934 reads in 978.391 CPU sec, 56.491 real sec [M::process] read 1925896 sequences (240000184 bp)... [M::process] 0 single-end sequences; 1925896 paired-end sequences [M::process] read 1925928 sequences (240000134 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1237, 751642, 39, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 105, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (122.01, 73.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 139, 2621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7789) [M::mem_pestat] mean and std.dev: (1206.82, 1585.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10373) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (108, 177, 1230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3474) [M::mem_pestat] mean and std.dev: (690.35, 888.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4596) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925896 reads in 998.927 CPU sec, 62.804 real sec [M::process] 0 single-end sequences; 1925928 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1322, 752220, 53, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 108, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (121.92, 72.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 86, 2270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6762) [M::mem_pestat] mean and std.dev: (1088.32, 1698.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9008) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (78, 185, 922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2610) [M::mem_pestat] mean and std.dev: (522.10, 702.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3454) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925928 reads in 966.742 CPU sec, 43.952 real sec [M::process] read 1925878 sequences (240000154 bp)... [M::process] 0 single-end sequences; 1925878 paired-end sequences [M::process] read 1925860 sequences (240000088 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1212, 752744, 46, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 106, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (122.38, 71.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (66, 252, 2354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6930) [M::mem_pestat] mean and std.dev: (1255.36, 1698.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9218) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (78, 129, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (142.66, 120.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925878 reads in 1000.851 CPU sec, 63.306 real sec [M::process] 0 single-end sequences; 1925860 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1223, 752646, 51, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 107, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (122.74, 71.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 167, 2610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7742) [M::mem_pestat] mean and std.dev: (1129.12, 1670.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10308) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 251, 1122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3060) [M::mem_pestat] mean and std.dev: (651.76, 745.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4029) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925860 reads in 939.797 CPU sec, 41.473 real sec [M::process] read 1925918 sequences (240000168 bp)... [M::process] 0 single-end sequences; 1925918 paired-end sequences [M::process] read 1925918 sequences (240000028 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1255, 751680, 54, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 117, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (128.08, 73.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.32, 30.11) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 136, 1477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4363) [M::mem_pestat] mean and std.dev: (586.38, 1013.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5806) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (83, 215, 710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1964) [M::mem_pestat] mean and std.dev: (380.86, 493.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2591) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925918 reads in 993.555 CPU sec, 63.862 real sec [M::process] 0 single-end sequences; 1925918 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1275, 751725, 56, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 110, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (124.53, 72.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.11) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 139, 1492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4414) [M::mem_pestat] mean and std.dev: (689.90, 1269.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5875) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (69, 165, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 636) [M::mem_pestat] mean and std.dev: (158.36, 129.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 825) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925918 reads in 956.256 CPU sec, 42.122 real sec [M::process] read 1925872 sequences (240000086 bp)... [M::process] 0 single-end sequences; 1925872 paired-end sequences [M::process] read 1925922 sequences (240000124 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1292, 751504, 47, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 106, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (122.58, 73.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 154, 1352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4014) [M::mem_pestat] mean and std.dev: (453.62, 722.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5345) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (87, 207, 957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2697) [M::mem_pestat] mean and std.dev: (545.98, 685.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3567) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925872 reads in 990.947 CPU sec, 63.928 real sec [M::process] 0 single-end sequences; 1925922 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1228, 752095, 52, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 112, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (125.19, 73.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 120, 2023) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6039) [M::mem_pestat] mean and std.dev: (987.70, 1609.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8047) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (87, 174, 936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2634) [M::mem_pestat] mean and std.dev: (421.68, 551.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3483) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925922 reads in 962.592 CPU sec, 42.385 real sec [M::process] read 1925926 sequences (240000162 bp)... [M::process] 0 single-end sequences; 1925926 paired-end sequences [M::process] read 1925866 sequences (240000024 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1265, 752015, 57, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 107, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (124.79, 74.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 94, 1736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5154) [M::mem_pestat] mean and std.dev: (899.42, 1541.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7067) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (80, 180, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (141.22, 80.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925926 reads in 1002.978 CPU sec, 64.630 real sec [M::process] 0 single-end sequences; 1925866 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1275, 752105, 53, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 114, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (128.53, 75.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 108, 1966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5860) [M::mem_pestat] mean and std.dev: (1110.36, 1801.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8317) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (54, 150, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 846) [M::mem_pestat] mean and std.dev: (149.53, 154.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925866 reads in 960.196 CPU sec, 43.711 real sec [M::process] read 1925864 sequences (240000072 bp)... [M::process] 0 single-end sequences; 1925864 paired-end sequences [M::process] read 1925940 sequences (240000110 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1197, 752110, 48, 52) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 109, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (125.20, 73.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (61, 186, 1651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4831) [M::mem_pestat] mean and std.dev: (783.13, 1159.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6421) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (79, 167, 751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2095) [M::mem_pestat] mean and std.dev: (406.88, 519.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2767) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925864 reads in 1000.040 CPU sec, 62.618 real sec [M::process] 0 single-end sequences; 1925940 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1261, 751776, 43, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 106, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (121.90, 73.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 525) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 272, 4251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12687) [M::mem_pestat] mean and std.dev: (1759.47, 2352.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16905) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (149, 270, 1443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4031) [M::mem_pestat] mean and std.dev: (689.25, 750.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5325) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925940 reads in 963.539 CPU sec, 43.661 real sec [M::process] read 1925870 sequences (240000234 bp)... [M::process] 0 single-end sequences; 1925870 paired-end sequences [M::process] read 1925952 sequences (240000008 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1233, 752503, 46, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 119, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (131.37, 74.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.32, 30.21) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 60, 1212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3608) [M::mem_pestat] mean and std.dev: (399.48, 854.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4806) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (88, 182, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2830) [M::mem_pestat] mean and std.dev: (409.73, 563.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3744) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925870 reads in 1003.429 CPU sec, 63.563 real sec [M::process] 0 single-end sequences; 1925952 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1239, 751978, 55, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 118, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (129.55, 74.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 87, 1508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4470) [M::mem_pestat] mean and std.dev: (573.06, 1121.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5951) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (79, 216, 817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2293) [M::mem_pestat] mean and std.dev: (392.98, 520.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3031) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925952 reads in 940.881 CPU sec, 41.549 real sec [M::process] read 1925888 sequences (240000036 bp)... [M::process] 0 single-end sequences; 1925888 paired-end sequences [M::process] read 1925900 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1249, 751472, 48, 43) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 110, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (125.29, 72.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 207, 2444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7266) [M::mem_pestat] mean and std.dev: (1240.79, 1628.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9677) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (112, 194, 1082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3022) [M::mem_pestat] mean and std.dev: (569.95, 677.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3992) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925888 reads in 996.055 CPU sec, 63.356 real sec [M::process] 0 single-end sequences; 1925900 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1252, 751408, 45, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 114, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (126.37, 73.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 534) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.42, 30.08) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 198, 4383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13103) [M::mem_pestat] mean and std.dev: (1724.27, 2168.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17463) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (104, 208, 947) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2633) [M::mem_pestat] mean and std.dev: (486.07, 569.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3476) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925900 reads in 963.888 CPU sec, 42.564 real sec [M::process] read 1925902 sequences (240000024 bp)... [M::process] 0 single-end sequences; 1925902 paired-end sequences [M::process] read 1925918 sequences (240000188 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1226, 751656, 41, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 113, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (127.39, 74.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 152, 4364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13036) [M::mem_pestat] mean and std.dev: (1553.78, 2084.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17372) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (118, 191, 1271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3577) [M::mem_pestat] mean and std.dev: (605.70, 837.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4730) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925902 reads in 998.282 CPU sec, 64.061 real sec [M::process] 0 single-end sequences; 1925918 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1248, 751429, 48, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 112, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (125.14, 72.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.43, 30.10) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 164, 4380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13082) [M::mem_pestat] mean and std.dev: (1632.42, 2504.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17433) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 209, 962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2624) [M::mem_pestat] mean and std.dev: (423.77, 520.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3455) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925918 reads in 954.115 CPU sec, 41.961 real sec [M::process] read 1925852 sequences (240000124 bp)... [M::process] 0 single-end sequences; 1925852 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1251, 752444, 50, 37) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 116, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (128.97, 74.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.33, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 102, 714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2116) [M::mem_pestat] mean and std.dev: (200.95, 412.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2817) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (107, 187, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 689) [M::mem_pestat] mean and std.dev: (181.28, 138.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 883) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925852 reads in 965.445 CPU sec, 53.190 real sec [M::process] read 1925940 sequences (240000214 bp)... [M::process] 0 single-end sequences; 1925940 paired-end sequences [M::process] read 1926016 sequences (240000040 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1266, 752644, 52, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 111, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (125.51, 72.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.42, 30.11) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 106, 2691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8031) [M::mem_pestat] mean and std.dev: (1257.20, 1994.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10701) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (108, 209, 438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1098) [M::mem_pestat] mean and std.dev: (243.80, 233.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1428) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925940 reads in 1001.603 CPU sec, 62.585 real sec [M::process] 0 single-end sequences; 1926016 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1252, 752396, 58, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 114, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (127.19, 73.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.42, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 150, 1502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4446) [M::mem_pestat] mean and std.dev: (826.44, 1310.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6068) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (56, 183, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 710) [M::mem_pestat] mean and std.dev: (128.06, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 928) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1926016 reads in 951.858 CPU sec, 43.072 real sec [M::process] read 1925890 sequences (240000138 bp)... [M::process] 0 single-end sequences; 1925890 paired-end sequences [M::process] read 1925962 sequences (240000084 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1318, 751250, 49, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 113, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (124.91, 71.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 43, 4374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13098) [M::mem_pestat] mean and std.dev: (1294.08, 1867.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17460) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (108, 203, 932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2580) [M::mem_pestat] mean and std.dev: (385.05, 418.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3404) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925890 reads in 990.236 CPU sec, 64.623 real sec [M::process] 0 single-end sequences; 1925962 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1309, 751041, 50, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (60, 106, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 408) [M::mem_pestat] mean and std.dev: (123.31, 73.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 524) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 85, 2323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6909) [M::mem_pestat] mean and std.dev: (1132.52, 1667.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9202) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (60, 131, 235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (121.10, 98.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925962 reads in 964.423 CPU sec, 42.572 real sec [M::process] read 1925824 sequences (240000034 bp)... [M::process] 0 single-end sequences; 1925824 paired-end sequences [M::process] read 1925904 sequences (240000050 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1235, 752012, 49, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 116, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 444) [M::mem_pestat] mean and std.dev: (130.12, 75.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.20) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 64, 1151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3409) [M::mem_pestat] mean and std.dev: (277.24, 620.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4538) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (113, 179, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 593) [M::mem_pestat] mean and std.dev: (149.62, 81.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925824 reads in 982.894 CPU sec, 65.166 real sec [M::process] 0 single-end sequences; 1925904 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1257, 751843, 55, 38) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 108, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (125.79, 75.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 161, 2669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7955) [M::mem_pestat] mean and std.dev: (1309.94, 1674.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10598) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (106, 209, 747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2029) [M::mem_pestat] mean and std.dev: (463.03, 543.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2670) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925904 reads in 961.219 CPU sec, 42.258 real sec [M::process] read 1925876 sequences (240000134 bp)... [M::process] 0 single-end sequences; 1925876 paired-end sequences [M::process] read 1925770 sequences (240000198 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1283, 751210, 42, 45) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 106, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (122.26, 72.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.29, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (8, 63, 1035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3089) [M::mem_pestat] mean and std.dev: (446.68, 878.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4116) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (97, 162, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 556) [M::mem_pestat] mean and std.dev: (166.46, 104.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 709) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925876 reads in 997.353 CPU sec, 62.904 real sec [M::process] 0 single-end sequences; 1925770 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1311, 751742, 35, 51) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 109, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (123.57, 73.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.32, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (68, 210, 3661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10847) [M::mem_pestat] mean and std.dev: (2016.86, 2622.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14440) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (123, 172, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 591) [M::mem_pestat] mean and std.dev: (153.68, 74.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925770 reads in 947.997 CPU sec, 41.712 real sec [M::process] read 1925868 sequences (240000236 bp)... [M::process] 0 single-end sequences; 1925868 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1304, 751710, 55, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 114, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (127.17, 73.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.32, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 254, 3719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11093) [M::mem_pestat] mean and std.dev: (1726.27, 2347.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14780) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (117, 221, 1797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5157) [M::mem_pestat] mean and std.dev: (767.35, 1136.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6837) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925868 reads in 990.933 CPU sec, 67.053 real sec [M::process] read 1925914 sequences (240000060 bp)... [M::process] 0 single-end sequences; 1925914 paired-end sequences [M::process] read 1925858 sequences (240000006 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1240, 751795, 53, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 112, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (127.30, 74.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 136, 2648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7898) [M::mem_pestat] mean and std.dev: (1186.50, 1798.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10523) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (60, 218, 1362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3966) [M::mem_pestat] mean and std.dev: (760.94, 1019.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5268) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925914 reads in 1003.915 CPU sec, 62.925 real sec [M::process] 0 single-end sequences; 1925858 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1258, 751594, 54, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 119, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (128.18, 70.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 232, 3066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9154) [M::mem_pestat] mean and std.dev: (1519.41, 1962.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12198) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 217, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1320) [M::mem_pestat] mean and std.dev: (275.94, 273.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1709) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925858 reads in 960.172 CPU sec, 43.551 real sec [M::process] read 1925948 sequences (240000026 bp)... [M::process] 0 single-end sequences; 1925948 paired-end sequences [M::process] read 1925864 sequences (240000134 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1271, 752585, 56, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 107, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (121.46, 71.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 410, 2651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7871) [M::mem_pestat] mean and std.dev: (1277.47, 1789.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10481) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (114, 212, 1061) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2955) [M::mem_pestat] mean and std.dev: (488.45, 516.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3902) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925948 reads in 979.411 CPU sec, 63.694 real sec [M::process] 0 single-end sequences; 1925864 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1256, 752051, 46, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 107, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (122.00, 71.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.38, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 146, 1215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3589) [M::mem_pestat] mean and std.dev: (419.32, 705.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4776) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 228, 920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2502) [M::mem_pestat] mean and std.dev: (364.00, 429.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3293) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925864 reads in 956.430 CPU sec, 42.207 real sec [M::process] read 1925938 sequences (240000112 bp)... [M::process] 0 single-end sequences; 1925938 paired-end sequences [M::process] read 1925930 sequences (240000240 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1215, 751832, 49, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 107, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 407) [M::mem_pestat] mean and std.dev: (122.72, 72.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 136, 1450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4296) [M::mem_pestat] mean and std.dev: (482.12, 961.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5719) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (84, 165, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1116) [M::mem_pestat] mean and std.dev: (193.78, 194.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1460) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925938 reads in 989.793 CPU sec, 64.839 real sec [M::process] 0 single-end sequences; 1925930 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1283, 751995, 39, 42) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 112, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (125.78, 71.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.32, 30.24) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 140, 1320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3890) [M::mem_pestat] mean and std.dev: (312.00, 646.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5175) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 203, 1026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2802) [M::mem_pestat] mean and std.dev: (438.87, 546.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3690) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925930 reads in 957.356 CPU sec, 42.167 real sec [M::process] read 1925920 sequences (240000020 bp)... [M::process] 0 single-end sequences; 1925920 paired-end sequences [M::process] read 1925906 sequences (240000054 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1233, 752615, 30, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 112, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (127.01, 74.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 403, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 1124, 2879) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8597) [M::mem_pestat] mean and std.dev: (1536.80, 1705.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11456) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (72, 137, 875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2481) [M::mem_pestat] mean and std.dev: (367.00, 510.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3284) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925920 reads in 993.328 CPU sec, 63.849 real sec [M::process] 0 single-end sequences; 1925906 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1288, 752371, 56, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 115, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (126.45, 73.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 152, 2856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8526) [M::mem_pestat] mean and std.dev: (1106.67, 1699.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11361) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (142, 231, 1087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2977) [M::mem_pestat] mean and std.dev: (573.22, 620.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3922) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925906 reads in 956.765 CPU sec, 42.054 real sec [M::process] read 1925900 sequences (240000030 bp)... [M::process] 0 single-end sequences; 1925900 paired-end sequences [M::process] read 1925924 sequences (240000016 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1236, 752170, 51, 55) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 108, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (126.24, 72.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.14) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 108, 1183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3499) [M::mem_pestat] mean and std.dev: (320.00, 609.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4657) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (97, 216, 566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1504) [M::mem_pestat] mean and std.dev: (313.66, 353.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1973) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925900 reads in 994.068 CPU sec, 64.134 real sec [M::process] 0 single-end sequences; 1925924 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1252, 752725, 40, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 112, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (126.53, 74.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.44, 30.08) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 185, 2663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7951) [M::mem_pestat] mean and std.dev: (1208.46, 1740.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10595) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (72, 179, 873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2475) [M::mem_pestat] mean and std.dev: (436.67, 551.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3276) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925924 reads in 951.457 CPU sec, 41.773 real sec [M::process] read 1925812 sequences (240000086 bp)... [M::process] 0 single-end sequences; 1925812 paired-end sequences [M::process] read 1925908 sequences (240000214 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1199, 752400, 69, 50) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (62, 112, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (124.28, 72.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.11) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 173, 3053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9107) [M::mem_pestat] mean and std.dev: (1548.97, 2241.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12134) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (122, 211, 1021) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2819) [M::mem_pestat] mean and std.dev: (536.62, 665.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3718) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925812 reads in 994.260 CPU sec, 62.792 real sec [M::process] 0 single-end sequences; 1925908 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1252, 752068, 46, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (69, 110, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 399) [M::mem_pestat] mean and std.dev: (126.23, 72.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (55, 753, 2748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8134) [M::mem_pestat] mean and std.dev: (1327.91, 1581.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10827) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (127, 209, 781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2089) [M::mem_pestat] mean and std.dev: (433.12, 518.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2743) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925908 reads in 964.558 CPU sec, 43.672 real sec [M::process] read 1925900 sequences (240000126 bp)... [M::process] 0 single-end sequences; 1925900 paired-end sequences [M::process] read 1925952 sequences (240000006 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1273, 752338, 39, 44) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 110, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (126.23, 72.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.42, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 164, 1618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4768) [M::mem_pestat] mean and std.dev: (911.47, 1492.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6881) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (99, 181, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065) [M::mem_pestat] mean and std.dev: (232.74, 246.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1387) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925900 reads in 998.802 CPU sec, 63.257 real sec [M::process] 0 single-end sequences; 1925952 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1300, 751973, 44, 33) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 107, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (123.58, 72.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 145, 2803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8353) [M::mem_pestat] mean and std.dev: (1460.28, 2286.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11128) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (153, 232, 1141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3117) [M::mem_pestat] mean and std.dev: (636.53, 769.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4105) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925952 reads in 951.754 CPU sec, 41.922 real sec [M::process] read 1925890 sequences (240000118 bp)... [M::process] 0 single-end sequences; 1925890 paired-end sequences [M::process] read 1925952 sequences (240000066 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1266, 750912, 46, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 110, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (125.57, 73.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.17) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 131, 3409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10171) [M::mem_pestat] mean and std.dev: (1596.07, 2429.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13552) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (129, 200, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1338) [M::mem_pestat] mean and std.dev: (325.05, 360.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1768) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925890 reads in 997.935 CPU sec, 63.461 real sec [M::process] 0 single-end sequences; 1925952 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1265, 752401, 53, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 108, 175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (122.67, 71.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.44, 30.11) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 175, 3834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11456) [M::mem_pestat] mean and std.dev: (1482.25, 1895.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15267) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (120, 208, 939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2577) [M::mem_pestat] mean and std.dev: (428.46, 471.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3396) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925952 reads in 945.267 CPU sec, 41.570 real sec [M::process] read 1925942 sequences (240000192 bp)... [M::process] 0 single-end sequences; 1925942 paired-end sequences [M::process] read 1925924 sequences (240000080 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1199, 752770, 48, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (67, 115, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (129.79, 74.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.19) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 366, 3627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10797) [M::mem_pestat] mean and std.dev: (1748.23, 2111.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14382) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (95, 239, 1574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4532) [M::mem_pestat] mean and std.dev: (787.95, 989.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6011) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925942 reads in 986.470 CPU sec, 63.971 real sec [M::process] 0 single-end sequences; 1925924 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1260, 752147, 40, 46) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 109, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (125.05, 73.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.40, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 139, 4393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13119) [M::mem_pestat] mean and std.dev: (1576.72, 2148.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17482) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (131, 196, 1251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3491) [M::mem_pestat] mean and std.dev: (593.70, 687.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4611) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925924 reads in 960.700 CPU sec, 42.200 real sec [M::process] read 1925910 sequences (240000152 bp)... [M::process] 0 single-end sequences; 1925910 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1243, 752480, 46, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 111, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (124.32, 73.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.37, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 371, 2242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6666) [M::mem_pestat] mean and std.dev: (1219.77, 1682.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8878) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (115, 199, 801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2173) [M::mem_pestat] mean and std.dev: (433.14, 486.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2859) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925910 reads in 1004.719 CPU sec, 62.678 real sec [M::process] read 1926006 sequences (240000198 bp)... [M::process] 0 single-end sequences; 1926006 paired-end sequences [M::process] read 1925916 sequences (240000178 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1260, 751680, 59, 39) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 107, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (123.19, 72.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 152, 2347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6985) [M::mem_pestat] mean and std.dev: (1131.12, 1672.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9304) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (97, 161, 274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 628) [M::mem_pestat] mean and std.dev: (172.57, 114.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1926006 reads in 1006.110 CPU sec, 62.559 real sec [M::process] 0 single-end sequences; 1925916 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1240, 751971, 52, 41) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 114, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (128.15, 75.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.36, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 123, 1945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5799) [M::mem_pestat] mean and std.dev: (1022.35, 1523.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7726) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (108, 189, 839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2301) [M::mem_pestat] mean and std.dev: (428.77, 504.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3032) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925916 reads in 963.796 CPU sec, 43.509 real sec [M::process] read 1925880 sequences (240000162 bp)... [M::process] 0 single-end sequences; 1925880 paired-end sequences [M::process] read 1925968 sequences (240000038 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1283, 751370, 58, 48) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (66, 117, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (127.61, 72.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.34, 30.20) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 138, 2953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8811) [M::mem_pestat] mean and std.dev: (1287.81, 2022.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11740) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (105, 385, 1111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3123) [M::mem_pestat] mean and std.dev: (653.56, 702.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4129) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925880 reads in 997.178 CPU sec, 63.877 real sec [M::process] 0 single-end sequences; 1925968 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1272, 752245, 42, 32) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 113, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (126.37, 73.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.42, 30.13) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 106, 2886) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8604) [M::mem_pestat] mean and std.dev: (1552.07, 2418.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11463) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (162, 211, 933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2475) [M::mem_pestat] mean and std.dev: (406.47, 445.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3246) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925968 reads in 961.983 CPU sec, 42.429 real sec [M::process] read 1925914 sequences (240000114 bp)... [M::process] 0 single-end sequences; 1925914 paired-end sequences [M::process] read 1925868 sequences (240000242 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1177, 752266, 48, 58) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (65, 114, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (128.61, 74.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.10) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 174, 2758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8206) [M::mem_pestat] mean and std.dev: (1310.19, 1853.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10930) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (133, 198, 917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2485) [M::mem_pestat] mean and std.dev: (498.88, 607.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3269) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925914 reads in 990.705 CPU sec, 63.348 real sec [M::process] 0 single-end sequences; 1925868 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1244, 752123, 52, 40) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 114, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 406) [M::mem_pestat] mean and std.dev: (128.81, 74.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.29, 30.18) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 149, 2267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6759) [M::mem_pestat] mean and std.dev: (1059.28, 1621.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9005) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (123, 212, 992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2730) [M::mem_pestat] mean and std.dev: (536.16, 727.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3599) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925868 reads in 969.685 CPU sec, 44.224 real sec [M::process] read 1925890 sequences (240000240 bp)... [M::process] 0 single-end sequences; 1925890 paired-end sequences [M::process] read 1925886 sequences (240000046 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1240, 751472, 43, 54) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 118, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (129.24, 73.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.41, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 94, 2642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7872) [M::mem_pestat] mean and std.dev: (1154.90, 1699.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10487) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (157, 271, 1189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3253) [M::mem_pestat] mean and std.dev: (572.96, 614.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4285) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925890 reads in 994.614 CPU sec, 62.797 real sec [M::process] 0 single-end sequences; 1925886 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1254, 751330, 47, 56) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (63, 105, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 423) [M::mem_pestat] mean and std.dev: (123.21, 73.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.35, 30.16) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 54, 825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2451) [M::mem_pestat] mean and std.dev: (195.69, 344.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3264) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (108, 182, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1218) [M::mem_pestat] mean and std.dev: (247.14, 260.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1588) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925886 reads in 964.911 CPU sec, 42.500 real sec [M::process] read 1925936 sequences (240000058 bp)... [M::process] 0 single-end sequences; 1925936 paired-end sequences [M::process] read 1925906 sequences (240000106 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1264, 752220, 39, 47) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (64, 105, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 403) [M::mem_pestat] mean and std.dev: (122.39, 72.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.42, 30.09) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (63, 225, 2535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7479) [M::mem_pestat] mean and std.dev: (1202.84, 1766.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9951) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (139, 330, 1131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3115) [M::mem_pestat] mean and std.dev: (660.57, 731.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4107) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925936 reads in 988.306 CPU sec, 64.442 real sec [M::process] 0 single-end sequences; 1925906 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1191, 751392, 57, 49) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (61, 114, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 412) [M::mem_pestat] mean and std.dev: (123.75, 72.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.39, 30.15) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 175, 1649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4913) [M::mem_pestat] mean and std.dev: (821.74, 1338.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6545) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (138, 232, 828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2208) [M::mem_pestat] mean and std.dev: (511.60, 543.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2898) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1925906 reads in 963.611 CPU sec, 42.501 real sec [M::process] read 367610 sequences (45811766 bp)... [M::process] 0 single-end sequences; 367610 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (224, 143301, 6, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (71, 113, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (130.45, 71.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (378, 402, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (292, 507) [M::mem_pestat] mean and std.dev: (403.32, 30.26) [M::mem_pestat] low and high boundaries for proper pairs: (249, 550) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_process_seqs] Processed 367610 reads in 204.094 CPU sec, 9.016 real sec [main] Version: 0.7.17-r1188 [main] CMD: /home/csyk2proj/tools/bwa.kit/bwa mem -p -t24 -R@RG\tID:KPGP-00318\tPL:ILLUMINA\tSM:KPGP-00318 -C /home/csyk2proj/data/bwa.kit/hs38DH.fa - [main] Real time: 39132.170 sec; CPU: 415829.583 sec