[M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671265, 39, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 655) [M::mem_pestat] mean and std.dev: (390.80, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (19, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 32, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (38.23, 39.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.263 CPU sec, 63.766 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 672150, 51, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.56, 79.93) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 35, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1224) [M::mem_pestat] mean and std.dev: (83.73, 194.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1626) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1010.507 CPU sec, 45.100 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671424, 41, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.54, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 54, 2242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6666) [M::mem_pestat] mean and std.dev: (1135.22, 1671.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8878) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1033.947 CPU sec, 62.937 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671730, 41, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.54, 80.04) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 53, 1362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4042) [M::mem_pestat] mean and std.dev: (388.28, 784.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5382) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1013.111 CPU sec, 44.939 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671851, 60, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.63, 79.98) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 46, 1029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3039) [M::mem_pestat] mean and std.dev: (330.38, 654.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4044) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1030.762 CPU sec, 62.704 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671903, 43, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.61, 80.04) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 61, 1761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5225) [M::mem_pestat] mean and std.dev: (980.85, 1536.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7127) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1014.958 CPU sec, 45.064 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671715, 41, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.50, 80.00) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 33, 659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1939) [M::mem_pestat] mean and std.dev: (230.53, 372.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2579) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1028.873 CPU sec, 63.193 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671632, 46, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.50, 80.06) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 59, 658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1930) [M::mem_pestat] mean and std.dev: (219.87, 446.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2566) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1018.206 CPU sec, 45.307 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671588, 49, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.47, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 30, 1290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3842) [M::mem_pestat] mean and std.dev: (274.90, 608.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5118) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.407 CPU sec, 64.196 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 672103, 45, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.64, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 42, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 391) [M::mem_pestat] mean and std.dev: (41.20, 32.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1007.935 CPU sec, 44.700 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 671881, 44, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.62, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 42, 1196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3560) [M::mem_pestat] mean and std.dev: (587.67, 937.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4742) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.101 CPU sec, 63.509 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671707, 49, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.53, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 52, 1082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3208) [M::mem_pestat] mean and std.dev: (418.77, 720.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4271) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1007.229 CPU sec, 44.687 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670975, 33, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.52, 80.06) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 37, 1013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3003) [M::mem_pestat] mean and std.dev: (343.00, 714.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3998) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1053.551 CPU sec, 58.480 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671652, 40, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.60, 80.25) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 40, 396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1144) [M::mem_pestat] mean and std.dev: (89.38, 181.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1518) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.765 CPU sec, 63.998 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671593, 45, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.48, 80.12) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 81, 1977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5873) [M::mem_pestat] mean and std.dev: (915.33, 1425.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7821) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1017.102 CPU sec, 45.248 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671265, 42, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 305, 458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 972) [M::mem_pestat] mean and std.dev: (253.00, 115.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1229) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.62, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 77, 1189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3533) [M::mem_pestat] mean and std.dev: (403.24, 798.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4705) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.880 CPU sec, 65.033 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671563, 56, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (315, 512, 1342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3396) [M::mem_pestat] mean and std.dev: (795.36, 522.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.36, 80.05) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 37, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (52.18, 69.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1067) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1019.581 CPU sec, 45.268 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 672229, 54, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.61, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 43, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1325) [M::mem_pestat] mean and std.dev: (97.78, 181.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1760) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.521 CPU sec, 64.819 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671991, 47, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.57, 80.17) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 54, 241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 693) [M::mem_pestat] mean and std.dev: (73.58, 118.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 919) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1012.215 CPU sec, 44.808 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 672088, 46, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.49, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 32, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (62.17, 89.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1034.915 CPU sec, 64.487 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671737, 55, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.55, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 47, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (61.63, 99.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1148, 1269, 2961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6587) [M::mem_pestat] mean and std.dev: (1990.09, 1146.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8400) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1016.207 CPU sec, 45.189 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 672486, 47, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (260, 376, 519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037) [M::mem_pestat] mean and std.dev: (357.00, 214.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1296) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.55, 80.06) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 38, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (44.86, 57.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1034.928 CPU sec, 64.378 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671470, 35, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.49, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 41, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990) [M::mem_pestat] mean and std.dev: (80.29, 143.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1316) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1009.233 CPU sec, 44.858 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 672031, 36, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (290, 448, 1245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3155) [M::mem_pestat] mean and std.dev: (773.00, 849.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4170) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.64, 80.20) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 41, 3262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9758) [M::mem_pestat] mean and std.dev: (1290.78, 2131.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13006) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1028.233 CPU sec, 63.690 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671483, 58, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.42, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 36, 1065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3161) [M::mem_pestat] mean and std.dev: (491.65, 878.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4209) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1010.044 CPU sec, 44.908 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 672158, 47, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.48, 80.22) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 45, 1069) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3161) [M::mem_pestat] mean and std.dev: (341.22, 754.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4207) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.416 CPU sec, 65.167 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671808, 52, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.72, 80.37) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 54, 3728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11132) [M::mem_pestat] mean and std.dev: (1783.02, 2823.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14834) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1002.701 CPU sec, 44.397 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671945, 49, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.51, 80.17) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 30, 670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1992) [M::mem_pestat] mean and std.dev: (169.50, 368.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2653) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1086.395 CPU sec, 65.389 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671899, 51, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.47, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 55, 393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1141) [M::mem_pestat] mean and std.dev: (54.13, 69.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1515) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1028.591 CPU sec, 45.540 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670981, 41, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.43, 80.04) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 46, 1448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4300) [M::mem_pestat] mean and std.dev: (379.00, 900.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5726) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1057.778 CPU sec, 65.021 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671995, 65, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.67, 80.12) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 45, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1353) [M::mem_pestat] mean and std.dev: (86.25, 165.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1798) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1031.178 CPU sec, 45.773 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671274, 41, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 326, 619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1383) [M::mem_pestat] mean and std.dev: (369.80, 303.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.60, 80.25) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 42, 510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1488) [M::mem_pestat] mean and std.dev: (97.30, 208.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1977) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1069.896 CPU sec, 65.021 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671372, 43, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.51, 80.07) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 43, 1140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3380) [M::mem_pestat] mean and std.dev: (402.10, 758.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4500) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (575, 1252, 2032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4946) [M::mem_pestat] mean and std.dev: (1152.90, 777.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6403) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1029.401 CPU sec, 45.647 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671575, 53, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.48, 80.12) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 49, 1761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5237) [M::mem_pestat] mean and std.dev: (824.88, 1478.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6975) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1055.304 CPU sec, 64.516 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671676, 37, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 387, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.78, 79.93) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 31, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (38.90, 39.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1024.460 CPU sec, 45.460 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 672434, 50, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.58, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 48, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 452) [M::mem_pestat] mean and std.dev: (65.49, 83.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1054.232 CPU sec, 65.086 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671783, 46, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.57, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 49, 1925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5727) [M::mem_pestat] mean and std.dev: (835.28, 1314.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7628) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1020.907 CPU sec, 45.344 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671266, 46, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.43, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 54, 1524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4536) [M::mem_pestat] mean and std.dev: (662.30, 1193.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6042) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1056.452 CPU sec, 64.532 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 672049, 45, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (491, 855, 1060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2198) [M::mem_pestat] mean and std.dev: (724.80, 375.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.63, 79.97) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 38, 1178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3494) [M::mem_pestat] mean and std.dev: (365.38, 782.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4652) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1019.361 CPU sec, 45.345 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671762, 41, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 349, 581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1329) [M::mem_pestat] mean and std.dev: (322.67, 207.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.41, 80.19) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 52, 1109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3291) [M::mem_pestat] mean and std.dev: (357.97, 716.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4382) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (207, 630, 860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2166) [M::mem_pestat] mean and std.dev: (681.60, 512.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2819) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1067.174 CPU sec, 60.002 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671701, 32, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (485, 737, 1348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3074) [M::mem_pestat] mean and std.dev: (879.55, 532.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3937) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.52, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 32, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (44.48, 55.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.893 CPU sec, 64.506 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671630, 47, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.66, 80.19) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 43, 1166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3474) [M::mem_pestat] mean and std.dev: (479.72, 885.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4628) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1022.562 CPU sec, 45.377 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671205, 53, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.52, 79.93) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 33, 67) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 169) [M::mem_pestat] mean and std.dev: (30.21, 26.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 220) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1050.379 CPU sec, 64.130 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 672004, 47, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.41, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 42, 1068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3174) [M::mem_pestat] mean and std.dev: (272.02, 539.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4227) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1019.647 CPU sec, 45.387 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671452, 38, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.57, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 48, 909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2679) [M::mem_pestat] mean and std.dev: (264.12, 496.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3564) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1051.150 CPU sec, 64.853 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671759, 53, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.55, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 65, 1968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5858) [M::mem_pestat] mean and std.dev: (878.12, 1453.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7803) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1021.874 CPU sec, 45.348 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671666, 43, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.41, 80.26) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 36, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 243) [M::mem_pestat] mean and std.dev: (31.27, 25.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 319) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.402 CPU sec, 64.542 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671440, 48, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.36, 80.04) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 97, 899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2651) [M::mem_pestat] mean and std.dev: (270.63, 518.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3527) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1020.598 CPU sec, 45.404 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671573, 43, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.45, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 50, 1065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3153) [M::mem_pestat] mean and std.dev: (405.69, 790.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4197) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1048.196 CPU sec, 64.544 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671552, 48, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.47, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 74, 1524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4514) [M::mem_pestat] mean and std.dev: (684.27, 1118.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6009) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1018.546 CPU sec, 45.276 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671754, 47, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.44, 80.17) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 49, 1217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3597) [M::mem_pestat] mean and std.dev: (524.82, 906.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4787) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1045.557 CPU sec, 64.320 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671496, 49, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 655) [M::mem_pestat] mean and std.dev: (390.77, 80.21) [M::mem_pestat] low and high boundaries for proper pairs: (19, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 56, 776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2274) [M::mem_pestat] mean and std.dev: (196.05, 481.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3023) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1017.435 CPU sec, 45.214 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671630, 40, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 387, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 655) [M::mem_pestat] mean and std.dev: (390.85, 80.14) [M::mem_pestat] low and high boundaries for proper pairs: (19, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 56, 1162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3424) [M::mem_pestat] mean and std.dev: (331.88, 628.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4555) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1046.362 CPU sec, 64.853 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671766, 54, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 331, 560) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1270) [M::mem_pestat] mean and std.dev: (326.44, 183.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.43, 80.17) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 43, 85) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 227) [M::mem_pestat] mean and std.dev: (35.56, 32.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 298) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1012.725 CPU sec, 45.063 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671196, 46, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.65, 80.31) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 52, 1172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3478) [M::mem_pestat] mean and std.dev: (252.71, 536.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4631) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1094.902 CPU sec, 66.248 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671843, 49, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.60, 80.03) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 46, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1313) [M::mem_pestat] mean and std.dev: (113.85, 240.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1745) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.912 CPU sec, 46.296 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 671493, 46, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 337, 1338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3850) [M::mem_pestat] mean and std.dev: (740.08, 851.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5106) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.59, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 78, 1533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4571) [M::mem_pestat] mean and std.dev: (798.95, 1401.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6405) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1058.790 CPU sec, 64.953 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 671773, 37, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 298, 619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1395) [M::mem_pestat] mean and std.dev: (399.25, 311.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.60, 80.06) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 29, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (29.90, 35.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 614) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1048.370 CPU sec, 46.518 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671757, 48, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.51, 80.23) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 37, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 563) [M::mem_pestat] mean and std.dev: (53.97, 84.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1062.203 CPU sec, 65.096 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 672028, 56, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.54, 79.96) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 38, 650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1918) [M::mem_pestat] mean and std.dev: (188.96, 413.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2552) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.006 CPU sec, 46.147 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 672503, 49, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.53, 80.00) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 119, 1355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4021) [M::mem_pestat] mean and std.dev: (546.47, 955.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5354) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1071.550 CPU sec, 65.590 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671483, 45, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.52, 80.21) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 53, 1259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3745) [M::mem_pestat] mean and std.dev: (541.17, 955.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4988) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.485 CPU sec, 46.379 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671597, 41, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.61, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 41, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (76.00, 94.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1077.346 CPU sec, 66.197 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671245, 44, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (298, 380, 664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1396) [M::mem_pestat] mean and std.dev: (404.90, 199.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1762) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.56, 80.17) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 38, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 441) [M::mem_pestat] mean and std.dev: (52.00, 76.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.159 CPU sec, 46.152 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671480, 41, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 387, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.76, 80.20) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 35, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 469) [M::mem_pestat] mean and std.dev: (49.38, 78.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 620) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1080.108 CPU sec, 66.500 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 672097, 44, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.52, 80.21) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 49, 1230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3634) [M::mem_pestat] mean and std.dev: (439.70, 840.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4836) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.937 CPU sec, 46.317 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671981, 45, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.59, 80.19) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 33, 99) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 277) [M::mem_pestat] mean and std.dev: (34.76, 36.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 366) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1062.486 CPU sec, 62.097 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671523, 49, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.48, 80.00) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 50, 1223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3621) [M::mem_pestat] mean and std.dev: (395.84, 641.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4820) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1054.176 CPU sec, 63.584 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671672, 46, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.61, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 73, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1212) [M::mem_pestat] mean and std.dev: (120.39, 217.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1608) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1050.648 CPU sec, 46.682 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671307, 36, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.58, 80.19) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 32, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 232) [M::mem_pestat] mean and std.dev: (36.19, 39.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 306) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1078.157 CPU sec, 65.169 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671900, 40, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.52, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 50, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2488) [M::mem_pestat] mean and std.dev: (184.18, 391.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3313) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.344 CPU sec, 46.456 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671218, 53, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.47, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 36, 1218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3624) [M::mem_pestat] mean and std.dev: (269.42, 707.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4827) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1059.746 CPU sec, 64.032 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 672266, 45, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.44, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 53, 1172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3462) [M::mem_pestat] mean and std.dev: (473.77, 822.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4607) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.612 CPU sec, 46.431 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 672089, 36, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (275, 415, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1658) [M::mem_pestat] mean and std.dev: (486.11, 400.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2119) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.54, 80.17) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 77, 2087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6215) [M::mem_pestat] mean and std.dev: (1052.14, 1737.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8279) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1070.247 CPU sec, 65.649 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 672146, 36, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (109, 1301, 1899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5479) [M::mem_pestat] mean and std.dev: (976.50, 850.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7269) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.45, 80.12) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 44, 1178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3498) [M::mem_pestat] mean and std.dev: (407.18, 735.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4658) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.049 CPU sec, 46.345 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 672005, 41, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.44, 80.19) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 46, 1067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3171) [M::mem_pestat] mean and std.dev: (329.32, 677.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4223) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1069.879 CPU sec, 65.537 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671534, 47, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.59, 80.24) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 39, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (50.19, 70.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1044.778 CPU sec, 46.557 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671699, 40, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.51, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 38, 1177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3505) [M::mem_pestat] mean and std.dev: (269.00, 682.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4669) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1072.448 CPU sec, 65.739 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671339, 45, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (300, 356, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (6, 741) [M::mem_pestat] mean and std.dev: (306.56, 99.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 888) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.57, 80.24) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 106, 1838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5458) [M::mem_pestat] mean and std.dev: (987.30, 1519.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7268) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1048.528 CPU sec, 46.548 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671397, 42, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.63, 80.32) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 63, 1033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3069) [M::mem_pestat] mean and std.dev: (451.08, 764.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4087) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1069.170 CPU sec, 65.761 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671984, 48, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.40, 80.12) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 35, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (43.32, 56.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 806) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.744 CPU sec, 46.254 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671729, 54, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.56, 80.24) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 49, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 826) [M::mem_pestat] mean and std.dev: (99.64, 151.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1096) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1068.268 CPU sec, 65.563 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 671565, 47, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (308, 409, 1018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2438) [M::mem_pestat] mean and std.dev: (539.77, 377.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3148) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.68, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 63, 1745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5193) [M::mem_pestat] mean and std.dev: (642.95, 1140.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6917) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.530 CPU sec, 46.196 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671360, 39, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.51, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 49, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 413) [M::mem_pestat] mean and std.dev: (49.61, 42.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 541) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1075.519 CPU sec, 65.350 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 672221, 54, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (102, 372, 999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2793) [M::mem_pestat] mean and std.dev: (499.70, 430.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.49, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 63, 1608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4780) [M::mem_pestat] mean and std.dev: (770.10, 1232.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6366) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.501 CPU sec, 46.193 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 672196, 51, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.53, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 50, 673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1971) [M::mem_pestat] mean and std.dev: (236.75, 489.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2620) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1075.339 CPU sec, 66.106 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 671734, 40, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.55, 80.17) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 51, 1272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3778) [M::mem_pestat] mean and std.dev: (444.27, 832.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5031) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.288 CPU sec, 46.100 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671899, 45, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.66, 80.21) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 39, 1343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3999) [M::mem_pestat] mean and std.dev: (329.08, 808.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5327) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1078.054 CPU sec, 66.533 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671294, 40, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 387, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 655) [M::mem_pestat] mean and std.dev: (390.88, 80.28) [M::mem_pestat] low and high boundaries for proper pairs: (19, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 104, 1745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5189) [M::mem_pestat] mean and std.dev: (887.84, 1334.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6911) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.852 CPU sec, 46.194 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 672310, 50, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 419, 2257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6433) [M::mem_pestat] mean and std.dev: (1165.70, 1438.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8521) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.52, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 28, 199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 573) [M::mem_pestat] mean and std.dev: (34.61, 44.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1067.381 CPU sec, 64.622 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671628, 56, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.55, 80.21) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 49, 935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2767) [M::mem_pestat] mean and std.dev: (340.58, 579.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3683) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.368 CPU sec, 46.243 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671907, 43, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.42, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 60, 1186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3502) [M::mem_pestat] mean and std.dev: (364.24, 728.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4660) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1066.954 CPU sec, 65.054 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 672054, 46, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.67, 80.20) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 33, 244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (82.97, 143.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.878 CPU sec, 46.299 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 671701, 50, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (286, 480, 1820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4888) [M::mem_pestat] mean and std.dev: (675.45, 639.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.58, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 48, 540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1576) [M::mem_pestat] mean and std.dev: (198.66, 345.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2094) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1075.615 CPU sec, 63.989 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 672207, 46, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.38, 80.12) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 58, 897) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2655) [M::mem_pestat] mean and std.dev: (234.54, 522.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3534) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1063.375 CPU sec, 64.891 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671630, 41, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.60, 80.38) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 43, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1581) [M::mem_pestat] mean and std.dev: (111.00, 241.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2102) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1044.704 CPU sec, 46.598 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 671001, 44, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 545, 1177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3075) [M::mem_pestat] mean and std.dev: (704.86, 613.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4024) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 387, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.67, 79.99) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 57, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1367) [M::mem_pestat] mean and std.dev: (137.56, 221.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1815) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.209 CPU sec, 65.066 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 672386, 53, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.53, 80.22) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 33, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (33.00, 30.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 379) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1044.949 CPU sec, 46.519 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 671946, 38, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 420, 956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2422) [M::mem_pestat] mean and std.dev: (489.42, 456.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3155) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.73, 80.28) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 43, 2880) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8608) [M::mem_pestat] mean and std.dev: (1277.05, 1635.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11472) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1080.132 CPU sec, 65.485 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 672040, 51, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (581, 975, 1397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3029) [M::mem_pestat] mean and std.dev: (843.58, 508.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3845) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.58, 80.24) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 48, 1199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3559) [M::mem_pestat] mean and std.dev: (363.55, 715.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4739) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1049.789 CPU sec, 46.644 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671565, 48, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (433, 842, 1505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3649) [M::mem_pestat] mean and std.dev: (1024.55, 650.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.60, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 37, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (44.41, 47.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1086.920 CPU sec, 66.258 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671083, 49, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.54, 80.27) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 21, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 222) [M::mem_pestat] mean and std.dev: (26.23, 32.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 293) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.606 CPU sec, 46.331 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 671584, 45, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.37, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 35, 803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2369) [M::mem_pestat] mean and std.dev: (176.47, 442.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3152) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (744, 1087, 2132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4908) [M::mem_pestat] mean and std.dev: (1260.40, 1165.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6296) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1063.150 CPU sec, 65.476 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671626, 37, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.65, 80.14) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 27, 65) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 171) [M::mem_pestat] mean and std.dev: (29.45, 30.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 224) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.024 CPU sec, 46.190 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671915, 45, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.59, 80.07) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 48, 1944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5784) [M::mem_pestat] mean and std.dev: (801.33, 1333.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7704) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1080.389 CPU sec, 66.098 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671431, 35, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.63, 80.24) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 38, 3116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9304) [M::mem_pestat] mean and std.dev: (1385.31, 2173.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12398) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.564 CPU sec, 46.274 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 672250, 48, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.66, 80.12) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 52, 558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1618) [M::mem_pestat] mean and std.dev: (141.80, 302.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2148) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1076.457 CPU sec, 65.682 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671930, 56, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (311, 392, 729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1565) [M::mem_pestat] mean and std.dev: (446.10, 265.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1983) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.62, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 39, 433) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1269) [M::mem_pestat] mean and std.dev: (149.71, 290.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1687) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.968 CPU sec, 46.237 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671267, 44, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.61, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 50, 922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2706) [M::mem_pestat] mean and std.dev: (197.27, 400.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3598) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1058.246 CPU sec, 64.622 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671944, 48, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.47, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 48, 139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (43.49, 35.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 484) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.638 CPU sec, 46.210 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671972, 57, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.41, 80.00) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 46, 1145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3413) [M::mem_pestat] mean and std.dev: (240.42, 557.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4547) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1065.144 CPU sec, 65.353 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 672445, 45, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.43, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 74, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 859) [M::mem_pestat] mean and std.dev: (91.03, 96.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1137) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1050.338 CPU sec, 46.632 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671771, 48, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (331, 418, 760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1618) [M::mem_pestat] mean and std.dev: (556.36, 356.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2047) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.45, 79.83) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 36, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 219) [M::mem_pestat] mean and std.dev: (43.21, 44.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 287) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1068.016 CPU sec, 65.827 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671419, 49, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.58, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 74, 2376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7088) [M::mem_pestat] mean and std.dev: (1222.94, 1776.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9444) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.610 CPU sec, 46.498 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671402, 45, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.52, 79.99) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 52, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (84.95, 130.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1111) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1068.397 CPU sec, 65.213 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 672148, 48, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.50, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 66, 1668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4954) [M::mem_pestat] mean and std.dev: (673.73, 1167.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6597) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.734 CPU sec, 46.492 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671734, 44, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.49, 80.24) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 52, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1180) [M::mem_pestat] mean and std.dev: (97.28, 182.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1567) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1075.271 CPU sec, 65.520 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 672081, 37, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.64, 79.97) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 73, 2908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8678) [M::mem_pestat] mean and std.dev: (1416.11, 2007.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11563) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1033.347 CPU sec, 45.950 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671917, 45, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.49, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 33, 108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (40.49, 43.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 372) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1062.702 CPU sec, 64.771 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 671489, 52, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 459, 1699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4601) [M::mem_pestat] mean and std.dev: (856.08, 813.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6052) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.49, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 44, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1248) [M::mem_pestat] mean and std.dev: (91.07, 199.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1659) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1050.419 CPU sec, 46.759 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671694, 56, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (284, 379, 803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1841) [M::mem_pestat] mean and std.dev: (486.00, 262.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2360) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.59, 80.33) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 46, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 543) [M::mem_pestat] mean and std.dev: (59.20, 69.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1075.080 CPU sec, 65.370 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671624, 41, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.56, 80.14) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 41, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1235) [M::mem_pestat] mean and std.dev: (111.80, 216.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1639) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1079.833 CPU sec, 65.414 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 672438, 38, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.59, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 33, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 838) [M::mem_pestat] mean and std.dev: (91.79, 166.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1112) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.524 CPU sec, 46.156 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 672437, 47, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.47, 80.00) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 51, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 819) [M::mem_pestat] mean and std.dev: (70.35, 95.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1084) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1074.607 CPU sec, 64.819 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671542, 49, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.62, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 42, 1056) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3130) [M::mem_pestat] mean and std.dev: (329.59, 633.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4167) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1054.183 CPU sec, 46.974 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671985, 38, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.52, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 57, 1532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4566) [M::mem_pestat] mean and std.dev: (864.46, 1362.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6315) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1066.300 CPU sec, 64.785 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671384, 49, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.75, 80.35) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 39, 1186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3522) [M::mem_pestat] mean and std.dev: (490.44, 959.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4690) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.957 CPU sec, 46.373 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 672174, 65, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.63, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 41, 1201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3573) [M::mem_pestat] mean and std.dev: (206.98, 558.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4759) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1071.345 CPU sec, 65.363 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671706, 45, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.46, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 133, 2723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8113) [M::mem_pestat] mean and std.dev: (1397.22, 1997.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10808) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.947 CPU sec, 46.375 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671603, 46, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.57, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 57, 867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2555) [M::mem_pestat] mean and std.dev: (290.98, 620.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3399) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1068.043 CPU sec, 65.660 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671919, 36, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.39, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 57, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (70.33, 101.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 845) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1045.325 CPU sec, 46.588 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671588, 45, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.39, 80.03) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 30, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249) [M::mem_pestat] mean and std.dev: (33.65, 39.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1077.643 CPU sec, 66.006 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 672305, 50, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.45, 80.01) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 62, 1446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4278) [M::mem_pestat] mean and std.dev: (553.69, 1048.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5694) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.200 CPU sec, 46.133 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671789, 48, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.56, 80.19) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 38, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1321) [M::mem_pestat] mean and std.dev: (77.08, 163.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1756) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1068.200 CPU sec, 66.301 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671782, 46, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.43, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 63, 1420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4214) [M::mem_pestat] mean and std.dev: (434.30, 848.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5611) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.844 CPU sec, 46.088 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671582, 44, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.55, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 48, 1260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3744) [M::mem_pestat] mean and std.dev: (472.10, 832.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4986) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1060.058 CPU sec, 64.904 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 672399, 49, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.58, 80.17) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 55, 876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2582) [M::mem_pestat] mean and std.dev: (297.84, 506.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3435) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1053.544 CPU sec, 46.779 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671614, 55, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.42, 80.04) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 50, 1030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3058) [M::mem_pestat] mean and std.dev: (287.12, 570.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4072) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1068.704 CPU sec, 65.050 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671368, 42, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.46, 80.23) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 71, 1854) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5526) [M::mem_pestat] mean and std.dev: (881.02, 1446.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7362) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.801 CPU sec, 46.338 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671919, 54, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.49, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 52, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1418) [M::mem_pestat] mean and std.dev: (130.91, 269.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1883) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1069.501 CPU sec, 65.480 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671990, 41, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (435, 623, 1065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2325) [M::mem_pestat] mean and std.dev: (764.55, 477.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2955) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.58, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 60, 1402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4142) [M::mem_pestat] mean and std.dev: (503.47, 838.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (927, 1138, 1539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2763) [M::mem_pestat] mean and std.dev: (1125.78, 385.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3375) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1037.944 CPU sec, 46.393 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 672071, 45, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 387, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 655) [M::mem_pestat] mean and std.dev: (390.80, 80.05) [M::mem_pestat] low and high boundaries for proper pairs: (19, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 66, 674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1980) [M::mem_pestat] mean and std.dev: (167.49, 333.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2633) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1082.908 CPU sec, 65.536 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671486, 41, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 757, 946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2460) [M::mem_pestat] mean and std.dev: (510.78, 347.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3217) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.45, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 61, 1657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4917) [M::mem_pestat] mean and std.dev: (872.90, 1456.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6699) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.164 CPU sec, 46.296 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671519, 49, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.58, 80.04) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 37, 1643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4893) [M::mem_pestat] mean and std.dev: (793.17, 1458.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6629) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1077.424 CPU sec, 66.405 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671742, 57, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.59, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 32, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1315) [M::mem_pestat] mean and std.dev: (81.96, 153.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1748) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.049 CPU sec, 46.230 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 671548, 45, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.72, 80.26) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 70, 1183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3505) [M::mem_pestat] mean and std.dev: (358.93, 652.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4666) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1062.231 CPU sec, 65.344 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671905, 53, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 408, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1334) [M::mem_pestat] mean and std.dev: (422.20, 284.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.60, 80.04) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 32, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (41.31, 53.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.330 CPU sec, 46.311 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671712, 48, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.54, 80.01) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 80, 1648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4890) [M::mem_pestat] mean and std.dev: (1025.30, 1531.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7153) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1058.705 CPU sec, 64.817 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671517, 44, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.49, 80.19) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 57, 286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 804) [M::mem_pestat] mean and std.dev: (86.97, 140.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (540, 1056, 1873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4539) [M::mem_pestat] mean and std.dev: (1169.78, 846.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5872) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1062.117 CPU sec, 64.499 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671337, 45, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (303, 330, 625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1269) [M::mem_pestat] mean and std.dev: (365.33, 116.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.50, 80.21) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 56, 704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2084) [M::mem_pestat] mean and std.dev: (274.12, 470.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2774) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.113 CPU sec, 46.399 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 672023, 54, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.42, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 49, 701) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2071) [M::mem_pestat] mean and std.dev: (244.09, 480.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2756) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1075.450 CPU sec, 65.814 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671868, 40, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.58, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 50, 805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2391) [M::mem_pestat] mean and std.dev: (350.57, 596.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3184) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1044.272 CPU sec, 46.483 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 672139, 46, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.54, 80.22) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 51, 955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2819) [M::mem_pestat] mean and std.dev: (316.82, 646.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3751) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1062.855 CPU sec, 65.225 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671344, 43, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.43, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 74, 1899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5667) [M::mem_pestat] mean and std.dev: (888.35, 1421.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7551) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.978 CPU sec, 46.402 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671884, 41, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.55, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 44, 1451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4309) [M::mem_pestat] mean and std.dev: (650.17, 1081.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5738) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1070.624 CPU sec, 65.541 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671788, 44, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.52, 80.00) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 56, 1202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3568) [M::mem_pestat] mean and std.dev: (413.50, 731.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4751) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.593 CPU sec, 46.233 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671629, 48, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 387, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.71, 79.97) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 85, 972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2870) [M::mem_pestat] mean and std.dev: (258.93, 480.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3819) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1072.229 CPU sec, 65.902 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 671768, 58, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.55, 80.12) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 46, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1750) [M::mem_pestat] mean and std.dev: (165.90, 329.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2327) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.649 CPU sec, 46.019 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 672347, 55, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.36, 80.04) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 30, 70) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 172) [M::mem_pestat] mean and std.dev: (32.67, 24.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 223) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1076.591 CPU sec, 65.896 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 672208, 44, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (287, 578, 899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2123) [M::mem_pestat] mean and std.dev: (506.11, 310.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.61, 80.14) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 40, 1943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5795) [M::mem_pestat] mean and std.dev: (1000.55, 1547.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7721) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.609 CPU sec, 46.304 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671512, 38, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.59, 80.17) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 48, 1321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3905) [M::mem_pestat] mean and std.dev: (477.76, 807.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5197) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1080.741 CPU sec, 66.048 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671614, 43, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 326, 1579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4383) [M::mem_pestat] mean and std.dev: (645.90, 822.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5785) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.58, 80.07) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 31, 78) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 192) [M::mem_pestat] mean and std.dev: (32.57, 27.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 249) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.675 CPU sec, 46.287 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 671594, 37, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.26, 80.05) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 38, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 989) [M::mem_pestat] mean and std.dev: (76.60, 131.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1313) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.774 CPU sec, 64.715 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 672041, 48, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.49, 80.01) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 55, 2454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7316) [M::mem_pestat] mean and std.dev: (1078.83, 1637.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9747) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1032.862 CPU sec, 45.871 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671469, 47, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.47, 80.04) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 41, 3596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10750) [M::mem_pestat] mean and std.dev: (1511.43, 2450.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14327) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1079.015 CPU sec, 66.111 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671532, 46, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.36, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 52, 1609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4785) [M::mem_pestat] mean and std.dev: (868.71, 1375.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6373) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1047.203 CPU sec, 46.663 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671828, 30, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.54, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 44, 1687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5025) [M::mem_pestat] mean and std.dev: (734.45, 1190.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6694) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1059.443 CPU sec, 64.345 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 672257, 48, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.49, 80.17) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 72, 2178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6486) [M::mem_pestat] mean and std.dev: (1152.68, 1792.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8640) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.731 CPU sec, 46.332 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 672333, 60, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 387, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.73, 80.24) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 48, 1001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2977) [M::mem_pestat] mean and std.dev: (415.71, 772.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3965) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1072.450 CPU sec, 65.967 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671962, 33, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.55, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 46, 2615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7801) [M::mem_pestat] mean and std.dev: (903.03, 1473.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10394) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1028.852 CPU sec, 45.822 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 671901, 41, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 313, 839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1993) [M::mem_pestat] mean and std.dev: (522.92, 416.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.40, 80.06) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 36, 138) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (43.06, 55.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 510) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1058.387 CPU sec, 64.997 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671175, 48, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.69, 80.17) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 38, 1047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3103) [M::mem_pestat] mean and std.dev: (202.88, 480.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4131) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1032.216 CPU sec, 45.914 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671461, 49, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.64, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 40, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (107.56, 213.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1165) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1057.490 CPU sec, 65.256 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671798, 37, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.60, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 37, 169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 477) [M::mem_pestat] mean and std.dev: (35.55, 34.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1031.999 CPU sec, 46.087 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671558, 26, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.63, 80.00) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 45, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (44.05, 43.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1085.569 CPU sec, 69.724 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671711, 30, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (335, 1073, 1159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2807) [M::mem_pestat] mean and std.dev: (659.11, 437.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.54, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 51, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (95.73, 137.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 654) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.025 CPU sec, 63.647 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 670955, 42, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.61, 80.23) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 39, 1512) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4504) [M::mem_pestat] mean and std.dev: (798.65, 1528.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6912) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1029.710 CPU sec, 45.810 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671456, 51, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.52, 80.23) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 53, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (74.55, 113.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1429) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1054.529 CPU sec, 64.574 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 672142, 47, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.37, 80.01) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 65, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1401) [M::mem_pestat] mean and std.dev: (158.95, 296.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1863) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1022.795 CPU sec, 45.423 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671285, 38, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.65, 80.31) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 48, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (47.10, 49.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.079 CPU sec, 64.455 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671789, 47, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (260, 1046, 1450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3830) [M::mem_pestat] mean and std.dev: (1133.60, 989.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5090) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.39, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 58, 1161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3437) [M::mem_pestat] mean and std.dev: (342.19, 645.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4575) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1020.032 CPU sec, 45.387 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 671414, 46, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (204, 374, 868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2196) [M::mem_pestat] mean and std.dev: (483.86, 475.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2860) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.37, 80.20) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 46, 1194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3540) [M::mem_pestat] mean and std.dev: (479.77, 781.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4713) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (214, 1038, 1190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3142) [M::mem_pestat] mean and std.dev: (877.56, 683.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4118) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1057.112 CPU sec, 65.555 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 671951, 41, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (303, 385, 789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1761) [M::mem_pestat] mean and std.dev: (456.50, 300.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2247) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.40, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 31, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (71.71, 131.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1018.991 CPU sec, 45.276 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671845, 40, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.64, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 56, 1097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3255) [M::mem_pestat] mean and std.dev: (323.00, 536.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4334) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1044.622 CPU sec, 64.804 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671900, 49, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.55, 80.07) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 72, 1339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3965) [M::mem_pestat] mean and std.dev: (522.95, 901.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5278) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1018.266 CPU sec, 45.179 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671427, 64, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.47, 80.22) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 46, 1886) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5610) [M::mem_pestat] mean and std.dev: (957.34, 1557.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7472) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.472 CPU sec, 64.759 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671710, 42, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.46, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 56, 1640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4892) [M::mem_pestat] mean and std.dev: (723.95, 1266.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6518) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1021.015 CPU sec, 45.560 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671748, 46, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 783, 908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2044) [M::mem_pestat] mean and std.dev: (658.80, 295.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.62, 80.14) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 36, 658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1946) [M::mem_pestat] mean and std.dev: (197.00, 411.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2590) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.398 CPU sec, 64.706 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671365, 36, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 387, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 655) [M::mem_pestat] mean and std.dev: (390.75, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (19, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 43, 1302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3874) [M::mem_pestat] mean and std.dev: (314.62, 631.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5160) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1013.350 CPU sec, 45.085 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671446, 44, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.59, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 34, 1537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4569) [M::mem_pestat] mean and std.dev: (735.12, 1393.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6309) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1047.616 CPU sec, 64.008 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671996, 48, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.49, 79.99) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 66, 2231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6645) [M::mem_pestat] mean and std.dev: (901.70, 1287.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8852) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1014.447 CPU sec, 45.051 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 672072, 34, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.54, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 36, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 176) [M::mem_pestat] mean and std.dev: (39.45, 32.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 230) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.068 CPU sec, 64.283 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671798, 47, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.52, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 40, 289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 805) [M::mem_pestat] mean and std.dev: (67.84, 115.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1020.614 CPU sec, 45.475 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 672564, 55, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.54, 80.17) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 49, 1608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4786) [M::mem_pestat] mean and std.dev: (891.64, 1479.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6811) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1045.025 CPU sec, 64.591 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671708, 47, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.38, 79.99) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 50, 1557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4625) [M::mem_pestat] mean and std.dev: (921.58, 1518.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6994) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1014.162 CPU sec, 45.135 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671582, 45, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 655) [M::mem_pestat] mean and std.dev: (390.70, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (19, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 55, 1291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3823) [M::mem_pestat] mean and std.dev: (439.07, 760.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5089) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1044.158 CPU sec, 64.290 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671288, 39, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (275, 370, 1820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4910) [M::mem_pestat] mean and std.dev: (724.40, 664.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.49, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 44, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1914) [M::mem_pestat] mean and std.dev: (77.90, 134.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2547) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1014.042 CPU sec, 45.092 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 672055, 41, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.49, 80.12) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 70, 1987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5893) [M::mem_pestat] mean and std.dev: (628.42, 1103.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7846) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.894 CPU sec, 64.499 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671906, 56, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.68, 79.99) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 55, 1862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5524) [M::mem_pestat] mean and std.dev: (915.75, 1524.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7355) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1010.543 CPU sec, 44.733 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671627, 49, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 387, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 655) [M::mem_pestat] mean and std.dev: (390.77, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (19, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 43, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1033) [M::mem_pestat] mean and std.dev: (99.34, 177.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1369) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.353 CPU sec, 64.435 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671267, 45, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.45, 80.14) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 52, 1061) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3123) [M::mem_pestat] mean and std.dev: (389.71, 725.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4154) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1017.115 CPU sec, 45.431 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671577, 42, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.51, 80.06) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 51, 989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2917) [M::mem_pestat] mean and std.dev: (277.25, 540.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3881) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1075.598 CPU sec, 69.724 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671855, 42, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.57, 80.03) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 51, 1075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3181) [M::mem_pestat] mean and std.dev: (450.15, 841.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4234) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1049.293 CPU sec, 64.714 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 671766, 41, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 353, 849) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1993) [M::mem_pestat] mean and std.dev: (432.00, 301.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2565) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.33, 80.01) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 48, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 945) [M::mem_pestat] mean and std.dev: (81.88, 141.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1253) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1022.329 CPU sec, 45.519 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671495, 52, 15) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.53, 80.00) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 33, 110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 298) [M::mem_pestat] mean and std.dev: (37.69, 40.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (766, 1090, 1819) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3925) [M::mem_pestat] mean and std.dev: (1047.00, 622.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4978) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1045.337 CPU sec, 64.677 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 672200, 47, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.51, 80.14) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 53, 918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2712) [M::mem_pestat] mean and std.dev: (290.83, 523.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3609) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1009.079 CPU sec, 44.762 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 672154, 36, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.54, 80.23) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 117, 2604) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7784) [M::mem_pestat] mean and std.dev: (1093.33, 1613.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10374) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1044.375 CPU sec, 64.738 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 672208, 52, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.47, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 44, 748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2212) [M::mem_pestat] mean and std.dev: (116.38, 267.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2944) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1015.351 CPU sec, 45.129 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671994, 50, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.51, 80.12) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 44, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1425) [M::mem_pestat] mean and std.dev: (151.79, 295.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1896) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.622 CPU sec, 64.286 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671458, 33, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.64, 80.06) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 48, 1237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3671) [M::mem_pestat] mean and std.dev: (265.38, 446.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4888) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1020.693 CPU sec, 45.405 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671733, 39, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 412, 708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1674) [M::mem_pestat] mean and std.dev: (423.22, 314.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2157) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.61, 80.04) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 34, 1238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3680) [M::mem_pestat] mean and std.dev: (267.82, 596.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4901) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.866 CPU sec, 64.133 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671622, 27, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.58, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 437, 1921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5729) [M::mem_pestat] mean and std.dev: (1234.67, 1518.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7633) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1009.598 CPU sec, 44.906 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671755, 47, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.44, 80.01) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 67, 1305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3857) [M::mem_pestat] mean and std.dev: (590.95, 977.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5133) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.191 CPU sec, 64.132 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671834, 42, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (350, 536, 1086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2558) [M::mem_pestat] mean and std.dev: (653.11, 420.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3294) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.48, 80.03) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 48, 1025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3033) [M::mem_pestat] mean and std.dev: (285.89, 644.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4037) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1010.897 CPU sec, 45.006 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 670994, 42, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (399, 438, 2818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7656) [M::mem_pestat] mean and std.dev: (1345.55, 1358.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.43, 80.21) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 34, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 648) [M::mem_pestat] mean and std.dev: (53.03, 111.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 858) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1045.172 CPU sec, 64.348 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671449, 54, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 387, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 655) [M::mem_pestat] mean and std.dev: (390.77, 80.03) [M::mem_pestat] low and high boundaries for proper pairs: (19, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 43, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 531) [M::mem_pestat] mean and std.dev: (37.80, 38.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 703) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1014.012 CPU sec, 45.180 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671595, 48, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.56, 80.19) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 32, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (31.89, 33.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 414) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.414 CPU sec, 63.969 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671785, 47, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 487, 831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2143) [M::mem_pestat] mean and std.dev: (449.67, 290.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2799) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.35, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 50, 1710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5092) [M::mem_pestat] mean and std.dev: (760.59, 1387.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6783) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1013.307 CPU sec, 45.396 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671049, 41, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.44, 80.12) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 41, 1952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5822) [M::mem_pestat] mean and std.dev: (875.23, 1385.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7757) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.255 CPU sec, 64.403 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 672054, 26, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 437, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (307.25, 117.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.55, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 173, 2624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7826) [M::mem_pestat] mean and std.dev: (1110.85, 1619.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10427) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1014.503 CPU sec, 45.203 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 672087, 56, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 1079, 1834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5014) [M::mem_pestat] mean and std.dev: (1082.40, 820.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6604) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.47, 80.21) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 32, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 313) [M::mem_pestat] mean and std.dev: (38.40, 47.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 412) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.828 CPU sec, 64.309 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 672216, 53, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.37, 80.12) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 66, 1578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4690) [M::mem_pestat] mean and std.dev: (787.37, 1336.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6246) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1011.812 CPU sec, 44.895 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 671653, 53, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 505, 1235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3257) [M::mem_pestat] mean and std.dev: (559.08, 504.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4268) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.57, 79.93) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 43, 1709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5075) [M::mem_pestat] mean and std.dev: (669.45, 1218.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6758) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1033.699 CPU sec, 63.969 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671558, 31, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.69, 80.20) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 38, 505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1477) [M::mem_pestat] mean and std.dev: (164.33, 287.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1963) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1018.231 CPU sec, 45.285 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 671814, 54, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.63, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 38, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 383) [M::mem_pestat] mean and std.dev: (45.26, 46.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 506) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.769 CPU sec, 64.306 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671619, 46, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (358, 485, 940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2104) [M::mem_pestat] mean and std.dev: (517.89, 277.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2686) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.47, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 38, 643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1903) [M::mem_pestat] mean and std.dev: (75.20, 140.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2533) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1008.971 CPU sec, 44.933 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671734, 42, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.41, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 51, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (46.78, 50.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 932) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.323 CPU sec, 64.321 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671526, 43, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.57, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 39, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 780) [M::mem_pestat] mean and std.dev: (66.86, 89.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1031) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1015.757 CPU sec, 45.187 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671687, 43, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.53, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 40, 1189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3545) [M::mem_pestat] mean and std.dev: (310.32, 544.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4723) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.992 CPU sec, 65.287 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 672066, 56, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.41, 79.95) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 82, 1155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3415) [M::mem_pestat] mean and std.dev: (426.60, 840.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4545) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1016.345 CPU sec, 45.147 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671362, 43, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.56, 80.03) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 37, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (46.49, 68.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.859 CPU sec, 63.664 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671826, 45, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 331, 883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2071) [M::mem_pestat] mean and std.dev: (608.91, 580.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2930) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.48, 80.22) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 42, 1691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5029) [M::mem_pestat] mean and std.dev: (714.21, 1349.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6698) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1011.047 CPU sec, 44.918 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 671564, 50, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (364, 529, 2222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5938) [M::mem_pestat] mean and std.dev: (1289.08, 1422.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7796) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.51, 80.02) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 53, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1142) [M::mem_pestat] mean and std.dev: (84.41, 140.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1516) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1035.989 CPU sec, 64.338 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 672170, 59, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 387, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.62, 79.95) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 45, 1084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3212) [M::mem_pestat] mean and std.dev: (321.43, 657.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4276) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1008.523 CPU sec, 44.829 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671707, 42, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.63, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 123, 1482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4400) [M::mem_pestat] mean and std.dev: (810.45, 1292.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5981) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.582 CPU sec, 64.471 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671269, 51, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.55, 80.12) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 34, 190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 546) [M::mem_pestat] mean and std.dev: (52.45, 95.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 724) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1020.252 CPU sec, 45.346 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671895, 51, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.51, 80.07) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 46, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1297) [M::mem_pestat] mean and std.dev: (135.24, 275.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1721) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1044.822 CPU sec, 64.467 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671404, 47, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.64, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 36, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1247) [M::mem_pestat] mean and std.dev: (131.54, 238.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1655) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1014.653 CPU sec, 45.012 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671626, 42, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 284, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 757) [M::mem_pestat] mean and std.dev: (269.11, 89.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 918) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.41, 80.03) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 35, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1291) [M::mem_pestat] mean and std.dev: (86.03, 142.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1715) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.919 CPU sec, 64.369 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671611, 48, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.50, 80.17) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 26, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1216) [M::mem_pestat] mean and std.dev: (38.43, 71.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1618) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1011.748 CPU sec, 45.031 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 672108, 41, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 262, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (309.30, 213.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1162) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.69, 80.20) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 42, 250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (63.06, 98.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 967) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.489 CPU sec, 64.728 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671770, 51, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 956, 2149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5837) [M::mem_pestat] mean and std.dev: (1326.90, 1312.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.67, 79.86) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 54, 932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2748) [M::mem_pestat] mean and std.dev: (311.13, 570.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3656) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1009.892 CPU sec, 44.916 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671929, 55, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.37, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 29, 423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1241) [M::mem_pestat] mean and std.dev: (133.19, 292.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1650) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1076.678 CPU sec, 61.112 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671672, 34, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.58, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 53, 624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1840) [M::mem_pestat] mean and std.dev: (224.17, 432.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2448) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1066.205 CPU sec, 65.226 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671092, 48, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 387, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.71, 80.36) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 38, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 777) [M::mem_pestat] mean and std.dev: (49.73, 57.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1033.943 CPU sec, 45.942 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 672086, 49, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.51, 79.96) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 60, 1222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3624) [M::mem_pestat] mean and std.dev: (448.53, 884.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4825) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.442 CPU sec, 64.883 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 672175, 48, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.63, 80.38) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 41, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (63.77, 109.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 981) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1023.245 CPU sec, 45.400 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 671300, 49, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 393, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 946) [M::mem_pestat] mean and std.dev: (309.80, 128.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.35, 80.17) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 50, 1971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5861) [M::mem_pestat] mean and std.dev: (924.67, 1308.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7806) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.329 CPU sec, 65.334 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671556, 53, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 655) [M::mem_pestat] mean and std.dev: (390.79, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (19, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 98, 3601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10747) [M::mem_pestat] mean and std.dev: (1767.17, 2740.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14320) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1023.930 CPU sec, 45.400 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671657, 43, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.42, 80.03) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 39, 621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1831) [M::mem_pestat] mean and std.dev: (179.36, 322.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2436) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1049.117 CPU sec, 64.673 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671795, 45, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.57, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 83, 1932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5752) [M::mem_pestat] mean and std.dev: (903.33, 1450.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7662) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1034.839 CPU sec, 45.996 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671119, 54, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.61, 80.36) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 36, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 291) [M::mem_pestat] mean and std.dev: (31.00, 27.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.136 CPU sec, 65.643 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671745, 37, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.49, 80.05) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 34, 102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248) [M::mem_pestat] mean and std.dev: (47.55, 50.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 321) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1034.147 CPU sec, 45.938 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671847, 41, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.57, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 46, 1171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3479) [M::mem_pestat] mean and std.dev: (412.38, 701.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4633) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1062.553 CPU sec, 65.056 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671755, 48, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.56, 79.97) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 45, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 699) [M::mem_pestat] mean and std.dev: (72.95, 133.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 926) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1025.173 CPU sec, 45.491 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671996, 42, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.51, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 38, 1460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4344) [M::mem_pestat] mean and std.dev: (595.39, 1068.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5786) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1062.140 CPU sec, 65.552 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671440, 48, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.58, 80.23) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 44, 862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2534) [M::mem_pestat] mean and std.dev: (322.73, 603.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3370) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1022.945 CPU sec, 45.455 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 672243, 52, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.39, 80.12) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 51, 1384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4104) [M::mem_pestat] mean and std.dev: (525.94, 974.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5464) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1062.858 CPU sec, 64.920 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 672028, 30, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.35, 80.07) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 59, 614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1760) [M::mem_pestat] mean and std.dev: (193.52, 357.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2333) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1044.105 CPU sec, 46.492 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671696, 50, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (185, 230, 918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2384) [M::mem_pestat] mean and std.dev: (444.00, 555.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3117) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.49, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 39, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (30.34, 32.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1060.251 CPU sec, 64.603 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 672257, 43, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.67, 79.95) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 59, 1164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3446) [M::mem_pestat] mean and std.dev: (398.69, 718.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4587) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1045.487 CPU sec, 46.595 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671907, 41, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.56, 80.22) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 35, 887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2633) [M::mem_pestat] mean and std.dev: (322.00, 622.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3506) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1070.771 CPU sec, 65.699 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671556, 50, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.59, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 55, 2728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8140) [M::mem_pestat] mean and std.dev: (1022.53, 1565.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10846) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.294 CPU sec, 46.276 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671818, 54, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.52, 80.19) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 51, 1079) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3187) [M::mem_pestat] mean and std.dev: (434.68, 737.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4241) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1081.055 CPU sec, 66.343 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 672003, 56, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.46, 80.22) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 55, 1193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3537) [M::mem_pestat] mean and std.dev: (423.56, 778.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4709) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.103 CPU sec, 46.244 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 672227, 51, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.42, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 44, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1835) [M::mem_pestat] mean and std.dev: (204.82, 400.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2438) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1072.502 CPU sec, 66.644 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671726, 47, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.52, 80.24) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 58, 1167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3441) [M::mem_pestat] mean and std.dev: (290.71, 603.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4578) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1035.729 CPU sec, 46.103 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 672130, 44, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.53, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 80, 1700) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5060) [M::mem_pestat] mean and std.dev: (896.47, 1454.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6740) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1083.937 CPU sec, 66.137 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 672076, 32, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 387, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.63, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 92, 1884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5598) [M::mem_pestat] mean and std.dev: (943.68, 1396.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7455) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.520 CPU sec, 46.293 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671373, 38, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 655) [M::mem_pestat] mean and std.dev: (390.72, 80.28) [M::mem_pestat] low and high boundaries for proper pairs: (19, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 103, 636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1838) [M::mem_pestat] mean and std.dev: (213.23, 374.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2439) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1070.766 CPU sec, 61.666 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671380, 48, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.58, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 49, 1124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3334) [M::mem_pestat] mean and std.dev: (396.81, 733.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4439) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1056.100 CPU sec, 64.284 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 671747, 52, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (291, 501, 790) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1788) [M::mem_pestat] mean and std.dev: (550.91, 415.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2287) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.62, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 42, 1202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3580) [M::mem_pestat] mean and std.dev: (346.34, 582.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4769) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.289 CPU sec, 46.323 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671350, 51, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.44, 80.22) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 29, 813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2401) [M::mem_pestat] mean and std.dev: (226.16, 512.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3195) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1086.208 CPU sec, 66.048 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 670898, 48, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.57, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 56, 2868) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8556) [M::mem_pestat] mean and std.dev: (1247.38, 1980.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11400) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.651 CPU sec, 46.498 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 672180, 39, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.51, 80.03) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 46, 1502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4464) [M::mem_pestat] mean and std.dev: (574.40, 1087.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5945) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1071.027 CPU sec, 65.282 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671567, 42, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.48, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 46, 1229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3647) [M::mem_pestat] mean and std.dev: (229.06, 498.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4856) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1049.116 CPU sec, 46.721 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671529, 30, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.51, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 60, 2107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6279) [M::mem_pestat] mean and std.dev: (1026.03, 1713.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8365) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.074 CPU sec, 64.689 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671618, 58, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.56, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 34, 920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2724) [M::mem_pestat] mean and std.dev: (260.64, 555.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3626) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1045.119 CPU sec, 46.478 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671558, 50, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (571, 943, 1543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3487) [M::mem_pestat] mean and std.dev: (918.50, 599.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4459) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.51, 80.19) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 41, 89) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 237) [M::mem_pestat] mean and std.dev: (39.68, 39.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 311) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1057.464 CPU sec, 64.226 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671746, 62, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.48, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 35, 668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1970) [M::mem_pestat] mean and std.dev: (172.38, 391.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2621) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1035.281 CPU sec, 46.147 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671717, 44, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (321, 411, 1493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3837) [M::mem_pestat] mean and std.dev: (828.18, 659.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5009) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.69, 80.23) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 54, 1122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3316) [M::mem_pestat] mean and std.dev: (330.40, 549.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4413) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1072.185 CPU sec, 65.738 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671699, 39, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.56, 80.28) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 29, 1447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4321) [M::mem_pestat] mean and std.dev: (419.35, 940.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5758) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1044.779 CPU sec, 46.467 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 671320, 47, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.31, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 41, 752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2204) [M::mem_pestat] mean and std.dev: (197.32, 438.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2930) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.767 CPU sec, 64.079 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 672193, 52, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (149, 339, 3472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10118) [M::mem_pestat] mean and std.dev: (1258.75, 1634.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13441) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.48, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 31, 1960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5860) [M::mem_pestat] mean and std.dev: (823.51, 1363.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7810) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1034.080 CPU sec, 46.077 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671203, 45, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.53, 80.14) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 51, 727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2145) [M::mem_pestat] mean and std.dev: (269.27, 556.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2854) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1068.153 CPU sec, 65.519 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671702, 42, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.36, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 38, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (57.89, 78.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1034.521 CPU sec, 45.873 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671589, 45, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 260, 930) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2358) [M::mem_pestat] mean and std.dev: (454.40, 474.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3072) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.55, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 65, 2370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7076) [M::mem_pestat] mean and std.dev: (983.41, 1585.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9429) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1078.832 CPU sec, 65.963 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671806, 40, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.47, 80.01) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 39, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1102) [M::mem_pestat] mean and std.dev: (52.45, 75.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1461) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.537 CPU sec, 46.364 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671519, 48, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.58, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 42, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (52.08, 51.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 598) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1077.500 CPU sec, 65.726 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671295, 48, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.44, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 44, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 891) [M::mem_pestat] mean and std.dev: (63.67, 111.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1183) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.583 CPU sec, 46.439 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 671872, 40, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (301, 380, 939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2215) [M::mem_pestat] mean and std.dev: (596.79, 447.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2853) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.55, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 29, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (30.91, 34.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 466) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1075.120 CPU sec, 66.101 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671601, 46, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 655) [M::mem_pestat] mean and std.dev: (390.71, 80.21) [M::mem_pestat] low and high boundaries for proper pairs: (19, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 51, 2702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8056) [M::mem_pestat] mean and std.dev: (1171.64, 1758.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10733) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (842, 1140, 1643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3245) [M::mem_pestat] mean and std.dev: (1021.25, 393.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4046) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1035.575 CPU sec, 46.058 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671798, 45, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 438, 1030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2466) [M::mem_pestat] mean and std.dev: (649.73, 576.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3184) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.52, 80.12) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 45, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 627) [M::mem_pestat] mean and std.dev: (52.80, 57.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1061.597 CPU sec, 65.619 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671208, 41, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.62, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 58, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1337) [M::mem_pestat] mean and std.dev: (135.06, 246.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1770) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1032.964 CPU sec, 46.039 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671365, 43, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.37, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 41, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7790) [M::mem_pestat] mean and std.dev: (1056.36, 1672.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10382) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1076.923 CPU sec, 65.796 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671861, 45, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (396, 440, 1336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3216) [M::mem_pestat] mean and std.dev: (649.50, 556.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4156) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.53, 80.24) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 64, 2243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6685) [M::mem_pestat] mean and std.dev: (821.63, 1247.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8906) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.265 CPU sec, 46.353 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671258, 53, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 211, 428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (219.78, 108.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.46, 79.96) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 52, 882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2598) [M::mem_pestat] mean and std.dev: (186.88, 376.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3456) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1080.479 CPU sec, 63.175 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 671801, 37, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (278, 298, 1814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4886) [M::mem_pestat] mean and std.dev: (927.92, 1151.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.52, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 48, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (95.77, 163.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1194) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1065.390 CPU sec, 65.030 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 672124, 45, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 990, 3832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10838) [M::mem_pestat] mean and std.dev: (1903.00, 2159.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14341) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.51, 80.06) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 36, 2725) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8135) [M::mem_pestat] mean and std.dev: (1027.91, 1677.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10840) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1048.000 CPU sec, 46.667 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671973, 46, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.51, 80.23) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 41, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1593) [M::mem_pestat] mean and std.dev: (199.60, 407.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2118) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (514, 1731, 2321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5935) [M::mem_pestat] mean and std.dev: (1513.00, 1115.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7742) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1069.309 CPU sec, 65.948 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671935, 41, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.54, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 56, 719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2105) [M::mem_pestat] mean and std.dev: (158.85, 304.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2798) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1051.692 CPU sec, 46.835 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 672135, 37, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 387, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.60, 80.05) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 62, 1159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3435) [M::mem_pestat] mean and std.dev: (501.83, 679.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4573) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1072.944 CPU sec, 65.975 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671728, 40, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.42, 80.19) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 102, 2369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7065) [M::mem_pestat] mean and std.dev: (1069.21, 1746.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9413) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.540 CPU sec, 46.391 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671557, 40, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.38, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 55, 2651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7905) [M::mem_pestat] mean and std.dev: (1111.97, 1629.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10532) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1073.568 CPU sec, 65.586 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671493, 40, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.35, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 36, 240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 682) [M::mem_pestat] mean and std.dev: (77.85, 122.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 903) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.067 CPU sec, 46.079 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671357, 53, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.60, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 41, 1067) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3161) [M::mem_pestat] mean and std.dev: (393.96, 764.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4208) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1077.152 CPU sec, 65.872 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 670569, 58, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 422, 1123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2903) [M::mem_pestat] mean and std.dev: (423.75, 349.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.52, 80.27) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 54, 1266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3766) [M::mem_pestat] mean and std.dev: (581.44, 942.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5016) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1052.828 CPU sec, 46.762 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 671905, 39, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.38, 80.04) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 35, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 566) [M::mem_pestat] mean and std.dev: (44.68, 55.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 750) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1083.226 CPU sec, 66.377 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 671786, 49, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 332, 1057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2677) [M::mem_pestat] mean and std.dev: (686.46, 627.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3487) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 387, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.65, 79.99) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 51, 1587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4723) [M::mem_pestat] mean and std.dev: (710.35, 1161.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6291) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.140 CPU sec, 46.426 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671644, 42, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.69, 80.04) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 51, 870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2562) [M::mem_pestat] mean and std.dev: (208.78, 402.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3408) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (285, 1136, 2089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5697) [M::mem_pestat] mean and std.dev: (1288.90, 1077.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7501) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1071.147 CPU sec, 66.061 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671348, 41, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.58, 80.07) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 39, 1226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3632) [M::mem_pestat] mean and std.dev: (515.03, 928.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4835) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.040 CPU sec, 46.331 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671664, 36, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (154, 836, 1325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3667) [M::mem_pestat] mean and std.dev: (639.89, 535.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4838) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.43, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 36, 99) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 267) [M::mem_pestat] mean and std.dev: (32.97, 31.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 351) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1060.687 CPU sec, 63.831 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671934, 54, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.48, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 40, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (51.39, 70.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.484 CPU sec, 46.175 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671997, 56, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 387, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.69, 79.97) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 31, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (49.70, 74.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 502) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1075.038 CPU sec, 65.604 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 672431, 31, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.68, 79.95) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 67, 1336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3948) [M::mem_pestat] mean and std.dev: (561.82, 1011.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5254) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.882 CPU sec, 46.191 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671918, 41, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.49, 80.06) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 46, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1327) [M::mem_pestat] mean and std.dev: (149.37, 296.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1764) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1070.934 CPU sec, 64.979 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 672361, 56, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (353, 924, 1311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3227) [M::mem_pestat] mean and std.dev: (913.20, 568.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4185) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.53, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 54, 390) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1122) [M::mem_pestat] mean and std.dev: (144.40, 251.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1488) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.791 CPU sec, 46.076 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 672233, 47, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.63, 80.20) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (8, 60, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (82.00, 139.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1232) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1078.609 CPU sec, 66.079 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 671527, 43, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 520, 1109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2703) [M::mem_pestat] mean and std.dev: (560.46, 408.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.76, 80.26) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 47, 2483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7413) [M::mem_pestat] mean and std.dev: (1210.45, 1972.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9878) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.004 CPU sec, 46.308 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671053, 49, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.61, 79.95) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 51, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1423) [M::mem_pestat] mean and std.dev: (127.24, 277.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1890) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1087.316 CPU sec, 66.116 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 672007, 47, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.56, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 68, 1450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4310) [M::mem_pestat] mean and std.dev: (632.18, 1078.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5740) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.942 CPU sec, 46.151 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671713, 56, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.55, 80.01) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 37, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1270) [M::mem_pestat] mean and std.dev: (86.36, 184.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1687) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1076.983 CPU sec, 65.447 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671958, 45, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.61, 80.02) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 42, 1419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4225) [M::mem_pestat] mean and std.dev: (489.95, 984.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5628) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1045.877 CPU sec, 46.623 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671355, 43, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 435, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 843) [M::mem_pestat] mean and std.dev: (359.50, 102.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.43, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 82, 1289) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3813) [M::mem_pestat] mean and std.dev: (677.71, 1064.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5075) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1072.121 CPU sec, 64.553 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671421, 34, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.62, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 40, 1905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5689) [M::mem_pestat] mean and std.dev: (867.34, 1389.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7581) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1057.056 CPU sec, 64.810 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671760, 49, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.67, 80.03) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 50, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7777) [M::mem_pestat] mean and std.dev: (1021.74, 1601.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10363) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.453 CPU sec, 46.119 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 672117, 47, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.55, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 47, 332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 934) [M::mem_pestat] mean and std.dev: (64.75, 74.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1235) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1062.479 CPU sec, 65.202 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671776, 49, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.40, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 72, 1575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4693) [M::mem_pestat] mean and std.dev: (852.85, 1414.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6511) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.030 CPU sec, 46.273 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671672, 40, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 655) [M::mem_pestat] mean and std.dev: (390.70, 80.19) [M::mem_pestat] low and high boundaries for proper pairs: (19, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 67, 2021) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6007) [M::mem_pestat] mean and std.dev: (700.42, 1180.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8000) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1069.571 CPU sec, 65.033 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 672291, 39, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 387, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.68, 80.07) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 66, 1818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5426) [M::mem_pestat] mean and std.dev: (928.05, 1454.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7230) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1028.209 CPU sec, 45.690 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671551, 53, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.53, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 44, 1155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3415) [M::mem_pestat] mean and std.dev: (486.65, 823.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4545) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1069.438 CPU sec, 65.651 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 672037, 57, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 387, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 655) [M::mem_pestat] mean and std.dev: (390.76, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (19, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 36, 2076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6190) [M::mem_pestat] mean and std.dev: (759.92, 1512.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8247) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.937 CPU sec, 46.420 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671463, 53, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.66, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 35, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 358) [M::mem_pestat] mean and std.dev: (38.45, 38.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 471) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1060.625 CPU sec, 64.973 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 672811, 54, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.66, 79.99) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 37, 1034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3062) [M::mem_pestat] mean and std.dev: (428.59, 853.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4076) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1032.658 CPU sec, 45.973 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671172, 47, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.55, 79.90) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 35, 678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2012) [M::mem_pestat] mean and std.dev: (223.88, 402.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2679) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1078.023 CPU sec, 66.000 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671934, 56, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.50, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 56, 2415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7199) [M::mem_pestat] mean and std.dev: (876.94, 1676.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9591) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.534 CPU sec, 46.358 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 670756, 42, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.55, 80.14) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 43, 2082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6216) [M::mem_pestat] mean and std.dev: (802.00, 1495.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8283) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1087.885 CPU sec, 66.431 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671121, 52, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.58, 80.07) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 33, 276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (67.43, 109.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1059) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1044.705 CPU sec, 46.457 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671890, 40, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 387, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.78, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 65, 1200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3554) [M::mem_pestat] mean and std.dev: (533.84, 818.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4731) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1065.611 CPU sec, 65.329 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671748, 38, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.57, 80.19) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 68, 1298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3848) [M::mem_pestat] mean and std.dev: (336.45, 722.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5123) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1028.629 CPU sec, 45.715 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671807, 42, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.68, 79.94) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 32, 705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2087) [M::mem_pestat] mean and std.dev: (164.06, 406.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2778) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1072.168 CPU sec, 65.301 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671614, 51, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 386, 1281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3543) [M::mem_pestat] mean and std.dev: (543.80, 492.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4674) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.52, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 51, 270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 772) [M::mem_pestat] mean and std.dev: (103.95, 161.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1023) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.504 CPU sec, 46.323 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671554, 40, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.51, 80.22) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 74, 1248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3696) [M::mem_pestat] mean and std.dev: (600.18, 1048.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4920) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1071.002 CPU sec, 65.601 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671788, 53, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.41, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 61, 1049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3107) [M::mem_pestat] mean and std.dev: (362.26, 707.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4136) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.587 CPU sec, 46.172 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671672, 42, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.58, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 124, 1377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4065) [M::mem_pestat] mean and std.dev: (567.27, 976.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5409) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1056.067 CPU sec, 64.659 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671242, 39, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.47, 80.06) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 61, 1909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5681) [M::mem_pestat] mean and std.dev: (769.83, 1256.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7567) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.743 CPU sec, 46.437 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671803, 47, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.45, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 78, 2761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8219) [M::mem_pestat] mean and std.dev: (1307.00, 1857.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10948) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1047.910 CPU sec, 64.754 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 672196, 43, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.62, 80.19) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 32, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (61.06, 82.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 939) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1031.299 CPU sec, 45.951 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671746, 43, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (370, 507, 1159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2737) [M::mem_pestat] mean and std.dev: (503.75, 315.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3526) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.50, 80.22) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 83, 2791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8313) [M::mem_pestat] mean and std.dev: (1197.38, 1629.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11074) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1066.535 CPU sec, 65.287 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671992, 46, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.41, 80.26) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 31, 54) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 120) [M::mem_pestat] mean and std.dev: (30.51, 17.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 153) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1033.177 CPU sec, 45.851 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 672003, 42, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.49, 80.17) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 54, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (65.88, 115.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1058.900 CPU sec, 65.112 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 671749, 51, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 715, 2406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6786) [M::mem_pestat] mean and std.dev: (1346.00, 1561.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8976) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.63, 80.05) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 52, 1136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3366) [M::mem_pestat] mean and std.dev: (407.78, 787.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4481) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1053.520 CPU sec, 64.357 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671504, 46, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.41, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 68, 1186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3506) [M::mem_pestat] mean and std.dev: (414.85, 690.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4666) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.687 CPU sec, 46.463 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 671526, 59, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 384, 1192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3172) [M::mem_pestat] mean and std.dev: (675.92, 571.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4162) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.50, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 40, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (62.72, 111.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1058.929 CPU sec, 64.186 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 672270, 44, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (393, 1300, 1571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3927) [M::mem_pestat] mean and std.dev: (1249.10, 846.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5105) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.47, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 49, 1644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4892) [M::mem_pestat] mean and std.dev: (688.56, 1234.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6516) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1035.432 CPU sec, 46.032 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671282, 43, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (321, 452, 1173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2877) [M::mem_pestat] mean and std.dev: (664.82, 478.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3729) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.34, 80.19) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 67, 1773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5257) [M::mem_pestat] mean and std.dev: (709.80, 1207.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6999) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1068.238 CPU sec, 64.717 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671137, 63, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.49, 80.19) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 30, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1175) [M::mem_pestat] mean and std.dev: (108.39, 221.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1562) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.537 CPU sec, 46.335 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 671989, 44, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (323, 666, 1037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2465) [M::mem_pestat] mean and std.dev: (508.27, 314.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3179) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.78, 80.32) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 77, 2117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6301) [M::mem_pestat] mean and std.dev: (885.79, 1423.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8393) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1056.286 CPU sec, 64.163 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671290, 38, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.45, 80.24) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 37, 1145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3399) [M::mem_pestat] mean and std.dev: (377.18, 707.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4526) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1026.419 CPU sec, 45.713 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671391, 39, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.59, 79.94) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 29, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 451) [M::mem_pestat] mean and std.dev: (43.35, 67.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 597) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1063.556 CPU sec, 65.044 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671763, 43, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.50, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 37, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 737) [M::mem_pestat] mean and std.dev: (47.94, 63.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 975) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1025.055 CPU sec, 45.631 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 672109, 45, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 387, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 655) [M::mem_pestat] mean and std.dev: (390.79, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (19, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 39, 1462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4354) [M::mem_pestat] mean and std.dev: (519.07, 978.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5800) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1062.028 CPU sec, 64.789 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671430, 51, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 655) [M::mem_pestat] mean and std.dev: (390.73, 80.06) [M::mem_pestat] low and high boundaries for proper pairs: (19, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 38, 1774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5282) [M::mem_pestat] mean and std.dev: (708.04, 1318.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7036) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1028.251 CPU sec, 45.727 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671706, 51, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.34, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 49, 910) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2678) [M::mem_pestat] mean and std.dev: (256.89, 460.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3562) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1061.165 CPU sec, 64.952 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671581, 53, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.45, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 45, 1547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4607) [M::mem_pestat] mean and std.dev: (708.18, 1182.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6137) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1022.492 CPU sec, 45.490 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671440, 50, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.53, 80.14) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 45, 1491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4437) [M::mem_pestat] mean and std.dev: (724.11, 1281.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5910) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1061.909 CPU sec, 64.417 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671563, 38, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.41, 80.03) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 33, 107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (48.19, 63.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 392) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1028.565 CPU sec, 45.843 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 672038, 52, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.74, 80.30) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 57, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1668) [M::mem_pestat] mean and std.dev: (95.00, 248.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2216) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.331 CPU sec, 64.942 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671572, 44, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.54, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 31, 191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 547) [M::mem_pestat] mean and std.dev: (45.37, 61.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 725) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1027.282 CPU sec, 45.729 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671633, 32, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.62, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 40, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (62.46, 76.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 948) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1056.304 CPU sec, 64.468 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 672030, 50, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.60, 80.14) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 69, 1185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3515) [M::mem_pestat] mean and std.dev: (474.69, 793.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4680) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1034.913 CPU sec, 46.070 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 672018, 45, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.51, 80.06) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 44, 1196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3564) [M::mem_pestat] mean and std.dev: (385.78, 694.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4748) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1059.777 CPU sec, 64.643 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671393, 40, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.67, 80.17) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 50, 243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 671) [M::mem_pestat] mean and std.dev: (64.19, 77.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1027.153 CPU sec, 45.945 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671893, 43, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 381, 1415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3803) [M::mem_pestat] mean and std.dev: (665.10, 630.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4997) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.48, 80.21) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 44, 698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2058) [M::mem_pestat] mean and std.dev: (186.81, 389.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2738) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1061.927 CPU sec, 64.938 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671180, 38, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (294, 730, 1119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2769) [M::mem_pestat] mean and std.dev: (807.70, 682.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.63, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 24, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1073) [M::mem_pestat] mean and std.dev: (31.10, 64.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1426) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1027.107 CPU sec, 45.711 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 671575, 36, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (311, 460, 1830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4868) [M::mem_pestat] mean and std.dev: (1090.33, 1037.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6387) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.62, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 43, 2245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6697) [M::mem_pestat] mean and std.dev: (992.00, 1524.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8923) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1055.007 CPU sec, 64.881 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671512, 56, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.57, 80.04) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 51, 1388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4108) [M::mem_pestat] mean and std.dev: (498.14, 906.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5468) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1023.849 CPU sec, 45.609 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671578, 37, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.34, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 39, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 587) [M::mem_pestat] mean and std.dev: (46.55, 44.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 775) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1076.622 CPU sec, 67.521 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671631, 44, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.32, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 59, 898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2642) [M::mem_pestat] mean and std.dev: (272.58, 524.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3514) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1058.062 CPU sec, 64.385 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 672411, 39, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.57, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 57, 865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2529) [M::mem_pestat] mean and std.dev: (246.71, 463.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3361) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.154 CPU sec, 46.115 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671531, 51, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.72, 80.22) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 36, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (32.82, 30.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1063.953 CPU sec, 65.357 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671620, 50, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.61, 80.00) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 42, 860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2530) [M::mem_pestat] mean and std.dev: (260.91, 478.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3365) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1034.015 CPU sec, 46.021 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671412, 40, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 287, 825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2085) [M::mem_pestat] mean and std.dev: (362.33, 285.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.59, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 39, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1213) [M::mem_pestat] mean and std.dev: (139.80, 287.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1611) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1054.421 CPU sec, 64.274 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 672021, 33, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.58, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 44, 1839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5495) [M::mem_pestat] mean and std.dev: (758.69, 1238.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7323) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.212 CPU sec, 46.134 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671412, 48, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (553, 901, 982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1840) [M::mem_pestat] mean and std.dev: (610.88, 315.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2269) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.52, 80.02) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 57, 1358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4032) [M::mem_pestat] mean and std.dev: (454.88, 878.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5369) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1057.112 CPU sec, 64.999 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671420, 44, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.64, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 49, 1882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5600) [M::mem_pestat] mean and std.dev: (814.17, 1376.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7459) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.187 CPU sec, 46.113 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671131, 49, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.54, 80.23) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 30, 1151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3421) [M::mem_pestat] mean and std.dev: (341.91, 680.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4556) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (722, 1557, 1592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3332) [M::mem_pestat] mean and std.dev: (1123.80, 621.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4202) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1063.647 CPU sec, 64.771 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671913, 38, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 655) [M::mem_pestat] mean and std.dev: (390.74, 80.23) [M::mem_pestat] low and high boundaries for proper pairs: (19, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 39, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (63.68, 97.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 970) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1031.195 CPU sec, 45.762 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671917, 51, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.49, 80.03) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 42, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (80.46, 122.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1059.288 CPU sec, 64.352 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 672103, 36, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.68, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 70, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1567) [M::mem_pestat] mean and std.dev: (98.38, 141.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2083) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1026.921 CPU sec, 45.575 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671714, 48, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.67, 80.12) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 64, 1189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3515) [M::mem_pestat] mean and std.dev: (357.51, 699.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4678) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1061.353 CPU sec, 65.562 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671897, 47, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 342, 609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1351) [M::mem_pestat] mean and std.dev: (368.11, 309.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.44, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 41, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (55.84, 60.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1026.173 CPU sec, 45.786 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 672181, 38, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.55, 80.03) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 30, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 714) [M::mem_pestat] mean and std.dev: (61.43, 107.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 947) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1062.910 CPU sec, 64.416 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671693, 51, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.49, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 38, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1288) [M::mem_pestat] mean and std.dev: (104.76, 249.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1711) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.899 CPU sec, 46.011 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671861, 52, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.47, 80.14) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 44, 1010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2986) [M::mem_pestat] mean and std.dev: (384.88, 770.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3974) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1074.663 CPU sec, 65.366 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671867, 40, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.46, 80.17) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 56, 1175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3501) [M::mem_pestat] mean and std.dev: (364.46, 770.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4664) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.082 CPU sec, 46.241 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 672351, 33, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.36, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 44, 913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2699) [M::mem_pestat] mean and std.dev: (255.40, 486.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3592) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1071.748 CPU sec, 65.487 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671857, 43, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (267, 689, 1228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3150) [M::mem_pestat] mean and std.dev: (612.78, 455.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4111) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 387, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.72, 79.99) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 52, 1565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4663) [M::mem_pestat] mean and std.dev: (766.00, 1303.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6212) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1034.433 CPU sec, 46.037 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671020, 49, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.51, 80.28) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 89, 2143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6379) [M::mem_pestat] mean and std.dev: (997.06, 1523.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8497) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.235 CPU sec, 64.746 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 672113, 52, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 939, 1214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3118) [M::mem_pestat] mean and std.dev: (837.80, 572.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4070) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.49, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 52, 1475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4371) [M::mem_pestat] mean and std.dev: (697.35, 1144.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5819) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.978 CPU sec, 46.162 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671831, 36, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.50, 80.17) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 46, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 914) [M::mem_pestat] mean and std.dev: (64.46, 87.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1208) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1061.315 CPU sec, 64.683 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671668, 47, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 340, 676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1552) [M::mem_pestat] mean and std.dev: (416.45, 299.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1990) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.49, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 38, 976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2900) [M::mem_pestat] mean and std.dev: (221.28, 535.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3862) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1031.160 CPU sec, 45.976 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671804, 51, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.54, 79.98) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 52, 1110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3270) [M::mem_pestat] mean and std.dev: (436.21, 770.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4350) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1070.551 CPU sec, 65.293 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 671958, 53, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 363, 662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1492) [M::mem_pestat] mean and std.dev: (306.90, 169.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1907) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.61, 79.93) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 66, 1266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3750) [M::mem_pestat] mean and std.dev: (561.00, 887.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4992) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1033.861 CPU sec, 45.943 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671606, 37, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.36, 80.25) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 48, 1223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3623) [M::mem_pestat] mean and std.dev: (637.26, 1066.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4901) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1067.538 CPU sec, 67.660 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671706, 48, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.59, 79.90) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 41, 668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1970) [M::mem_pestat] mean and std.dev: (123.72, 269.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2621) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1063.272 CPU sec, 64.398 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 672013, 46, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.51, 80.07) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 43, 202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (51.83, 68.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 760) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1027.486 CPU sec, 45.746 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671452, 44, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.61, 80.03) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 52, 898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2642) [M::mem_pestat] mean and std.dev: (342.55, 646.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3514) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1067.292 CPU sec, 64.954 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671797, 49, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.64, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 990) [M::mem_pestat] mean and std.dev: (81.85, 161.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1315) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.456 CPU sec, 46.336 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 671634, 37, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.43, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 41, 840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2482) [M::mem_pestat] mean and std.dev: (206.28, 449.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3303) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1067.163 CPU sec, 65.382 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671839, 49, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.48, 80.28) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 32, 804) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2388) [M::mem_pestat] mean and std.dev: (291.49, 531.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3180) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1033.794 CPU sec, 45.936 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671991, 44, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.50, 80.07) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 43, 743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2185) [M::mem_pestat] mean and std.dev: (147.56, 389.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2906) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1062.003 CPU sec, 65.327 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671881, 38, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.63, 80.23) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 86, 1251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3711) [M::mem_pestat] mean and std.dev: (472.06, 727.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4941) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1030.558 CPU sec, 45.827 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671870, 44, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.68, 79.91) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 49, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1040) [M::mem_pestat] mean and std.dev: (77.78, 130.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1382) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (953, 1307, 1900) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3794) [M::mem_pestat] mean and std.dev: (1285.67, 503.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4741) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1050.959 CPU sec, 64.832 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671862, 42, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.47, 80.20) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 75, 2740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8188) [M::mem_pestat] mean and std.dev: (1317.95, 1963.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10912) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1035.411 CPU sec, 46.439 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 672306, 45, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.42, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 36, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 740) [M::mem_pestat] mean and std.dev: (75.41, 142.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 982) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1059.408 CPU sec, 65.030 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 672066, 46, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.55, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 49, 1436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4268) [M::mem_pestat] mean and std.dev: (377.51, 755.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5684) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1034.421 CPU sec, 45.930 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671933, 49, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 896, 1689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4639) [M::mem_pestat] mean and std.dev: (940.33, 858.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6114) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.35, 80.01) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 33, 811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2415) [M::mem_pestat] mean and std.dev: (251.45, 578.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3217) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1058.470 CPU sec, 65.460 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 672184, 45, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.51, 79.99) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 46, 732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2142) [M::mem_pestat] mean and std.dev: (252.10, 474.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2847) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.182 CPU sec, 46.106 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671713, 47, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (295, 338, 1242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3136) [M::mem_pestat] mean and std.dev: (546.27, 465.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4083) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.43, 80.23) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 38, 1420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4216) [M::mem_pestat] mean and std.dev: (568.74, 1052.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5614) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1080.643 CPU sec, 65.532 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 672043, 49, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 717, 1556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4008) [M::mem_pestat] mean and std.dev: (1086.70, 1001.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5234) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.47, 80.00) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 57, 1738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5176) [M::mem_pestat] mean and std.dev: (838.87, 1483.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6895) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.028 CPU sec, 46.129 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671953, 43, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.43, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 29, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 494) [M::mem_pestat] mean and std.dev: (69.55, 103.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 653) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1066.027 CPU sec, 65.159 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 671855, 46, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 297, 1068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2804) [M::mem_pestat] mean and std.dev: (574.50, 495.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.50, 80.06) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 25, 587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1721) [M::mem_pestat] mean and std.dev: (105.62, 277.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2288) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.629 CPU sec, 46.272 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671644, 42, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.55, 79.99) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 48, 2038) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6076) [M::mem_pestat] mean and std.dev: (1045.61, 1716.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8095) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1067.739 CPU sec, 65.082 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 672550, 45, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 387, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 655) [M::mem_pestat] mean and std.dev: (390.81, 80.22) [M::mem_pestat] low and high boundaries for proper pairs: (19, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 40, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (78.28, 102.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 658) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1032.173 CPU sec, 45.823 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671395, 54, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 387, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.65, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 46, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 734) [M::mem_pestat] mean and std.dev: (70.27, 111.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 974) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1061.713 CPU sec, 65.115 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 672428, 39, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.52, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 104, 3477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10369) [M::mem_pestat] mean and std.dev: (1704.08, 2172.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13815) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1028.971 CPU sec, 45.924 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671249, 46, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.54, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 39, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1450) [M::mem_pestat] mean and std.dev: (177.56, 353.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1927) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1072.657 CPU sec, 66.000 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671205, 47, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.59, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 76, 1888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5610) [M::mem_pestat] mean and std.dev: (857.42, 1385.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7471) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.192 CPU sec, 46.047 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671437, 32, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (366, 753, 1895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4953) [M::mem_pestat] mean and std.dev: (1057.18, 1019.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6482) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.60, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 126, 2180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6478) [M::mem_pestat] mean and std.dev: (1018.45, 1428.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8627) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1066.953 CPU sec, 64.901 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671447, 39, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.48, 80.14) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 93, 2831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8445) [M::mem_pestat] mean and std.dev: (1389.36, 1910.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11252) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.544 CPU sec, 46.289 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671690, 43, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.55, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 35, 1142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3392) [M::mem_pestat] mean and std.dev: (372.00, 645.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4517) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (373, 812, 1206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2872) [M::mem_pestat] mean and std.dev: (891.10, 626.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3705) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1077.453 CPU sec, 66.891 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 672017, 43, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 455, 776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1972) [M::mem_pestat] mean and std.dev: (538.50, 433.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2570) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.56, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 53, 2056) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6108) [M::mem_pestat] mean and std.dev: (1085.19, 1580.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8134) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1034.447 CPU sec, 45.970 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671640, 50, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.42, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 51, 1574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4692) [M::mem_pestat] mean and std.dev: (630.86, 1223.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6251) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1072.404 CPU sec, 64.987 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671801, 42, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.46, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 66, 937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2775) [M::mem_pestat] mean and std.dev: (168.29, 370.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3694) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1044.950 CPU sec, 46.812 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671769, 42, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.63, 80.23) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 51) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 123) [M::mem_pestat] mean and std.dev: (26.26, 20.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 159) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1075.881 CPU sec, 66.145 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 672156, 51, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (375, 651, 1987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5211) [M::mem_pestat] mean and std.dev: (930.45, 978.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.52, 80.30) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 51, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1898) [M::mem_pestat] mean and std.dev: (162.33, 288.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2523) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.252 CPU sec, 46.479 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671738, 38, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 655) [M::mem_pestat] mean and std.dev: (390.76, 80.17) [M::mem_pestat] low and high boundaries for proper pairs: (19, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 51, 1049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3101) [M::mem_pestat] mean and std.dev: (244.42, 470.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4127) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1076.463 CPU sec, 65.487 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671741, 47, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.63, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 50, 1999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5959) [M::mem_pestat] mean and std.dev: (1074.57, 1656.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7939) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (509, 804, 1628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3866) [M::mem_pestat] mean and std.dev: (1045.20, 699.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4985) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1033.480 CPU sec, 45.928 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 672009, 30, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (320, 546, 2616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7208) [M::mem_pestat] mean and std.dev: (1417.77, 1778.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.59, 80.06) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 77, 1312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3906) [M::mem_pestat] mean and std.dev: (540.63, 1031.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5203) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.105 CPU sec, 65.183 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671679, 49, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.49, 80.27) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 39, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259) [M::mem_pestat] mean and std.dev: (46.30, 52.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 338) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1047.782 CPU sec, 46.722 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671563, 47, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.59, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 37, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1740) [M::mem_pestat] mean and std.dev: (106.35, 259.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2315) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1063.742 CPU sec, 65.026 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671464, 42, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 468, 1058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2524) [M::mem_pestat] mean and std.dev: (651.10, 488.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3257) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.67, 80.19) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 58, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 384) [M::mem_pestat] mean and std.dev: (46.44, 33.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 503) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.980 CPU sec, 46.666 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671904, 54, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.39, 80.24) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 32, 81) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 217) [M::mem_pestat] mean and std.dev: (33.98, 32.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 285) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1065.735 CPU sec, 65.268 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671933, 57, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.44, 80.07) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 41, 1052) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3116) [M::mem_pestat] mean and std.dev: (349.13, 694.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4148) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1034.442 CPU sec, 46.118 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671049, 42, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.53, 80.22) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 118, 2562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7636) [M::mem_pestat] mean and std.dev: (1370.40, 1841.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10173) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1050.630 CPU sec, 59.945 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671554, 47, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.48, 80.23) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 62, 611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1777) [M::mem_pestat] mean and std.dev: (236.14, 367.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2360) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1073.248 CPU sec, 64.989 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 672163, 43, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.65, 80.40) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 41, 1182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3508) [M::mem_pestat] mean and std.dev: (392.41, 810.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4671) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1044.886 CPU sec, 46.571 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671724, 44, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (377, 1126, 1165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2741) [M::mem_pestat] mean and std.dev: (750.11, 473.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.69, 80.36) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 40, 1318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3908) [M::mem_pestat] mean and std.dev: (376.38, 788.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5203) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1076.299 CPU sec, 64.884 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671537, 57, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.47, 80.04) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 45, 185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 517) [M::mem_pestat] mean and std.dev: (48.84, 48.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 683) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1029.805 CPU sec, 45.725 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671075, 51, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.69, 80.43) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 50, 257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 739) [M::mem_pestat] mean and std.dev: (53.40, 70.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1060.701 CPU sec, 64.440 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 672185, 40, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (506, 968, 1945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4823) [M::mem_pestat] mean and std.dev: (1151.10, 1021.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6262) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.60, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 38, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 401) [M::mem_pestat] mean and std.dev: (51.47, 69.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.594 CPU sec, 46.132 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671288, 51, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 378, 1058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2750) [M::mem_pestat] mean and std.dev: (514.56, 449.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3596) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.59, 80.05) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1206) [M::mem_pestat] mean and std.dev: (130.59, 259.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1603) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.129 CPU sec, 65.177 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671056, 48, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (337, 466, 855) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1891) [M::mem_pestat] mean and std.dev: (512.89, 234.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2409) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.48, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 45, 1210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3590) [M::mem_pestat] mean and std.dev: (613.91, 1059.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4851) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1045.077 CPU sec, 46.928 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671349, 43, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.45, 80.19) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 43, 1446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4308) [M::mem_pestat] mean and std.dev: (651.73, 1234.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5739) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1080.512 CPU sec, 66.207 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671827, 45, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.57, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 24, 109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (29.78, 33.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 400) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.835 CPU sec, 46.235 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671425, 53, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.62, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 54, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1238) [M::mem_pestat] mean and std.dev: (158.26, 267.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1642) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1074.214 CPU sec, 65.644 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671517, 28, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.57, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 39, 1233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3661) [M::mem_pestat] mean and std.dev: (353.79, 831.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4875) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.052 CPU sec, 46.235 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671427, 45, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.38, 80.12) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 43, 1516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4516) [M::mem_pestat] mean and std.dev: (580.66, 1054.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6016) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1061.630 CPU sec, 66.266 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671955, 35, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.56, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 72, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1131) [M::mem_pestat] mean and std.dev: (113.46, 199.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1497) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.275 CPU sec, 46.424 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671412, 41, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 453, 774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1902) [M::mem_pestat] mean and std.dev: (456.44, 353.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2466) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.56, 80.22) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 49, 1669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4981) [M::mem_pestat] mean and std.dev: (778.26, 1391.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6637) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1082.315 CPU sec, 66.488 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671438, 46, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 387, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 655) [M::mem_pestat] mean and std.dev: (390.82, 80.17) [M::mem_pestat] low and high boundaries for proper pairs: (19, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 36, 837) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2475) [M::mem_pestat] mean and std.dev: (201.82, 450.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3294) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.526 CPU sec, 46.466 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671302, 50, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 694, 1072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2716) [M::mem_pestat] mean and std.dev: (462.25, 353.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3538) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.58, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 48, 1186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3524) [M::mem_pestat] mean and std.dev: (380.44, 699.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4693) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1072.481 CPU sec, 65.388 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671627, 55, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.50, 80.05) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 41, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (64.18, 90.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 814) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1047.005 CPU sec, 46.628 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 672173, 39, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.50, 80.12) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 73, 1858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5526) [M::mem_pestat] mean and std.dev: (907.79, 1437.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7360) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1068.763 CPU sec, 65.320 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671931, 53, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.33, 80.00) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 46, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1112) [M::mem_pestat] mean and std.dev: (113.51, 204.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1475) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.978 CPU sec, 46.393 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 672077, 34, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 559, 844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1898) [M::mem_pestat] mean and std.dev: (606.78, 509.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.70, 80.17) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 32, 408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1198) [M::mem_pestat] mean and std.dev: (161.83, 337.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1593) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1070.807 CPU sec, 65.500 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671849, 39, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 443, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1159) [M::mem_pestat] mean and std.dev: (424.10, 237.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.49, 80.25) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 125, 1274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3764) [M::mem_pestat] mean and std.dev: (351.28, 505.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5009) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.294 CPU sec, 46.643 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671861, 40, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.47, 80.07) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 36, 805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2385) [M::mem_pestat] mean and std.dev: (286.82, 458.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3175) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1060.604 CPU sec, 64.921 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671938, 47, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.48, 80.18) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 46, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1123) [M::mem_pestat] mean and std.dev: (144.80, 245.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1489) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.575 CPU sec, 46.377 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671812, 51, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.52, 80.07) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 52, 1202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3554) [M::mem_pestat] mean and std.dev: (311.48, 670.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4730) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1065.428 CPU sec, 65.360 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671649, 50, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.52, 80.15) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 37, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 279) [M::mem_pestat] mean and std.dev: (49.09, 62.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 368) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1046.952 CPU sec, 46.602 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671292, 53, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.54, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 40, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1666) [M::mem_pestat] mean and std.dev: (134.36, 237.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2213) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.740 CPU sec, 60.386 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 672551, 42, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.59, 79.91) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 48, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 678) [M::mem_pestat] mean and std.dev: (61.76, 92.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 900) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1060.367 CPU sec, 65.242 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671171, 57, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.49, 80.08) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 37, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 912) [M::mem_pestat] mean and std.dev: (68.18, 124.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1212) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.673 CPU sec, 46.168 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 672551, 46, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 785, 1123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2815) [M::mem_pestat] mean and std.dev: (623.82, 452.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3661) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.61, 80.16) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 32, 1388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4134) [M::mem_pestat] mean and std.dev: (547.16, 1016.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5507) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1061.280 CPU sec, 65.199 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671475, 42, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.73, 80.02) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 31, 102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (31.39, 28.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.469 CPU sec, 46.261 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671888, 46, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.47, 80.30) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 46, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1812) [M::mem_pestat] mean and std.dev: (174.14, 384.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2409) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1072.362 CPU sec, 65.130 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 671885, 38, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 364, 693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1603) [M::mem_pestat] mean and std.dev: (377.83, 248.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2058) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.45, 80.19) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 55, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (61.71, 61.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.385 CPU sec, 46.044 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671587, 61, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.35, 80.12) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 68, 1198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3548) [M::mem_pestat] mean and std.dev: (287.08, 653.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4723) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1080.746 CPU sec, 65.841 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671537, 38, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.52, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 41, 1194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3554) [M::mem_pestat] mean and std.dev: (445.83, 842.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4734) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.206 CPU sec, 46.214 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671255, 39, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.39, 80.17) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (29.87, 30.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 579) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1061.572 CPU sec, 65.775 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671765, 49, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.66, 79.98) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 29, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 247) [M::mem_pestat] mean and std.dev: (32.41, 37.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 324) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.070 CPU sec, 46.083 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 672059, 45, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (127, 652) [M::mem_pestat] mean and std.dev: (390.61, 79.94) [M::mem_pestat] low and high boundaries for proper pairs: (22, 757) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 47, 1926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5740) [M::mem_pestat] mean and std.dev: (782.45, 1377.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7647) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1072.247 CPU sec, 65.687 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671489, 45, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.72, 80.37) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 34, 670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1978) [M::mem_pestat] mean and std.dev: (187.84, 407.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2632) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.984 CPU sec, 46.446 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671677, 48, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.52, 80.20) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 39, 834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2468) [M::mem_pestat] mean and std.dev: (195.45, 391.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3285) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1072.578 CPU sec, 65.731 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 671320, 39, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.57, 80.28) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 49, 1774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5268) [M::mem_pestat] mean and std.dev: (863.89, 1265.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7015) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.483 CPU sec, 46.172 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671453, 41, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.47, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 34, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (31.34, 29.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1070.767 CPU sec, 64.819 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 671727, 42, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.55, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 274, 2670) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7952) [M::mem_pestat] mean and std.dev: (1380.08, 1980.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10593) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.330 CPU sec, 46.486 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671367, 49, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.44, 80.14) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 52, 1505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4455) [M::mem_pestat] mean and std.dev: (554.44, 1106.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5930) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1069.473 CPU sec, 65.256 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671941, 46, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.53, 80.13) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 42, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1317) [M::mem_pestat] mean and std.dev: (132.79, 265.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1751) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1030.670 CPU sec, 45.869 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671435, 51, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.58, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 38, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1230) [M::mem_pestat] mean and std.dev: (135.47, 279.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1633) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1061.256 CPU sec, 64.814 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671658, 52, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.67, 80.09) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 55, 354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1016) [M::mem_pestat] mean and std.dev: (77.38, 142.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1347) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1034.933 CPU sec, 46.120 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671390, 55, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 657) [M::mem_pestat] mean and std.dev: (390.69, 80.24) [M::mem_pestat] low and high boundaries for proper pairs: (15, 764) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 39, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 707) [M::mem_pestat] mean and std.dev: (57.16, 77.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1074.271 CPU sec, 65.407 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 672289, 37, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.32, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 35, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1312) [M::mem_pestat] mean and std.dev: (167.00, 330.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1743) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1030.631 CPU sec, 46.211 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 672253, 48, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.46, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 35, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (44.77, 51.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1077.540 CPU sec, 66.013 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671662, 44, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.59, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 39, 1056) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3138) [M::mem_pestat] mean and std.dev: (427.93, 803.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4179) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1034.906 CPU sec, 45.969 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 672000, 38, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.62, 80.10) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 72, 607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1763) [M::mem_pestat] mean and std.dev: (308.74, 502.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2341) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1071.718 CPU sec, 65.285 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 671411, 56, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (310, 372, 996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2368) [M::mem_pestat] mean and std.dev: (612.54, 574.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3054) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (337, 386, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 655) [M::mem_pestat] mean and std.dev: (390.91, 80.28) [M::mem_pestat] low and high boundaries for proper pairs: (19, 761) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 58, 1600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4754) [M::mem_pestat] mean and std.dev: (920.34, 1409.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6559) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.866 CPU sec, 46.121 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1155740 sequences (173361000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 671535, 49, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.35, 80.14) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 40, 668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1964) [M::mem_pestat] mean and std.dev: (248.23, 497.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2612) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (707, 785, 1265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2381) [M::mem_pestat] mean and std.dev: (840.56, 343.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2939) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1060.720 CPU sec, 59.594 real sec [M::process] 0 single-end sequences; 1155740 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 485195, 37, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (336, 386, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 654) [M::mem_pestat] mean and std.dev: (390.56, 80.11) [M::mem_pestat] low and high boundaries for proper pairs: (18, 760) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 69, 1489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4427) [M::mem_pestat] mean and std.dev: (773.97, 1171.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5896) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1155740 reads in 772.109 CPU sec, 33.847 real sec [main] Version: 0.7.17-r1188 [main] CMD: /home/csyk2proj/tools/bwa.kit/bwa mem -p -t24 -R@RG\tID:KPGP-00328\tPL:ILLUMINA\tSM:KPGP-00328 -C /home/csyk2proj/data/bwa.kit/hs38DH.fa - [main] Real time: 44087.629 sec; CPU: 526518.202 sec