[M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671104, 49, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.85, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 35, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 550) [M::mem_pestat] mean and std.dev: (54.17, 70.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 727) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1120.007 CPU sec, 66.196 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671616, 45, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.01, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 36, 1783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5323) [M::mem_pestat] mean and std.dev: (723.88, 1378.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7093) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1069.955 CPU sec, 47.580 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 670743, 37, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.93, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 49, 303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (88.94, 170.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1161) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1103.142 CPU sec, 65.691 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670574, 41, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.91, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 30, 1878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5606) [M::mem_pestat] mean and std.dev: (634.74, 1334.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7470) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1073.212 CPU sec, 47.634 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 670916, 45, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (414, 803, 1255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2937) [M::mem_pestat] mean and std.dev: (820.33, 502.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.93, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 58, 1302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3872) [M::mem_pestat] mean and std.dev: (308.28, 571.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5157) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1079.752 CPU sec, 64.820 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670837, 43, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.00, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 41, 1353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4025) [M::mem_pestat] mean and std.dev: (560.24, 992.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5361) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1055.632 CPU sec, 46.938 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670824, 52, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.78, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 43, 162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 450) [M::mem_pestat] mean and std.dev: (44.24, 62.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 594) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1104.850 CPU sec, 65.870 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670783, 46, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 672) [M::mem_pestat] mean and std.dev: (398.02, 82.86) [M::mem_pestat] low and high boundaries for proper pairs: (12, 782) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 46, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (64.66, 71.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 567) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (686, 902, 1376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2756) [M::mem_pestat] mean and std.dev: (1011.08, 608.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3446) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1052.127 CPU sec, 46.749 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671460, 46, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.85, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 35, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 616) [M::mem_pestat] mean and std.dev: (38.94, 49.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1097.925 CPU sec, 66.163 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671196, 32, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.07, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 34, 59) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 147) [M::mem_pestat] mean and std.dev: (29.50, 18.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 191) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1059.359 CPU sec, 47.057 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 670633, 43, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.02, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 27, 136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 382) [M::mem_pestat] mean and std.dev: (28.58, 30.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 505) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (832, 1202, 2199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4933) [M::mem_pestat] mean and std.dev: (1381.58, 1051.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6300) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1087.121 CPU sec, 65.030 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670907, 37, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.89, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 48, 2269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6769) [M::mem_pestat] mean and std.dev: (783.56, 1392.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9019) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1077.179 CPU sec, 47.981 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670624, 41, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.95, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 42, 1242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3676) [M::mem_pestat] mean and std.dev: (336.50, 756.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4893) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1083.889 CPU sec, 60.085 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671497, 39, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.03, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 47, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 478) [M::mem_pestat] mean and std.dev: (57.29, 61.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 631) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1089.003 CPU sec, 66.178 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671000, 39, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.87, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 62, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1592) [M::mem_pestat] mean and std.dev: (161.00, 360.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2113) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1065.981 CPU sec, 47.360 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670568, 40, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.96, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 41, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 336) [M::mem_pestat] mean and std.dev: (35.87, 32.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1094.785 CPU sec, 65.420 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671330, 42, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (306, 1646, 3672) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10404) [M::mem_pestat] mean and std.dev: (2091.80, 2290.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13770) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.87, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 43, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1236) [M::mem_pestat] mean and std.dev: (90.41, 172.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1642) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1066.921 CPU sec, 47.363 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671407, 51, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (285, 994, 1360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3510) [M::mem_pestat] mean and std.dev: (732.60, 497.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.75, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 38, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 178) [M::mem_pestat] mean and std.dev: (35.55, 27.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 231) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1103.806 CPU sec, 65.447 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670660, 47, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.00, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 28, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (37.11, 43.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1069.077 CPU sec, 47.457 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670777, 46, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.06, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 38, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (41.42, 44.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 565) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1099.964 CPU sec, 65.618 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 670911, 46, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.82, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 49, 902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2674) [M::mem_pestat] mean and std.dev: (442.52, 742.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3560) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1070.483 CPU sec, 47.620 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670912, 40, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.92, 82.44) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 34, 1109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3297) [M::mem_pestat] mean and std.dev: (317.71, 757.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4391) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1103.125 CPU sec, 65.232 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670814, 37, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.80, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 48, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1395) [M::mem_pestat] mean and std.dev: (74.14, 106.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1853) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1069.953 CPU sec, 47.633 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 670996, 47, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.97, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 39, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 886) [M::mem_pestat] mean and std.dev: (78.03, 148.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1175) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1103.239 CPU sec, 65.659 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670992, 57, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.99, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 32, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 196) [M::mem_pestat] mean and std.dev: (36.75, 36.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 256) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.488 CPU sec, 47.310 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670905, 33, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.94, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 28, 57) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 149) [M::mem_pestat] mean and std.dev: (29.62, 24.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 195) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1081.516 CPU sec, 66.177 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671190, 46, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.98, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 31, 63) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 161) [M::mem_pestat] mean and std.dev: (33.51, 29.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 210) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1069.607 CPU sec, 47.511 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 670721, 40, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (268, 687, 1254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3226) [M::mem_pestat] mean and std.dev: (655.56, 512.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4212) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 672) [M::mem_pestat] mean and std.dev: (398.14, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (12, 782) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 30, 102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (40.30, 51.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 369) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1108.935 CPU sec, 65.563 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 670623, 45, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (414, 509, 836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1680) [M::mem_pestat] mean and std.dev: (522.80, 256.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2102) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.89, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 34, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (49.27, 66.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1075.813 CPU sec, 47.848 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 670391, 37, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (368, 518, 1745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4499) [M::mem_pestat] mean and std.dev: (588.22, 465.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5876) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.93, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 44, 2770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8254) [M::mem_pestat] mean and std.dev: (1112.25, 2008.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10996) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1107.113 CPU sec, 66.007 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 670881, 39, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.90, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 91, 2990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8914) [M::mem_pestat] mean and std.dev: (1651.92, 2689.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12411) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1057.026 CPU sec, 47.012 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671186, 42, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.84, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (45.53, 70.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 863) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1102.691 CPU sec, 65.748 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670467, 41, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.86, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 35, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (60.15, 86.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (328, 1274, 2279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6181) [M::mem_pestat] mean and std.dev: (1442.80, 1094.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8132) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1071.870 CPU sec, 47.672 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 670633, 45, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.80, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 40, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 309) [M::mem_pestat] mean and std.dev: (47.58, 50.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1099.705 CPU sec, 66.033 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670822, 49, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.90, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 69, 3454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10324) [M::mem_pestat] mean and std.dev: (1579.65, 2433.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13759) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1073.114 CPU sec, 47.788 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671051, 52, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.85, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 61, 1240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3682) [M::mem_pestat] mean and std.dev: (482.00, 847.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4903) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1106.075 CPU sec, 66.067 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670698, 47, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.82, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 26, 70) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 176) [M::mem_pestat] mean and std.dev: (28.79, 28.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 229) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.974 CPU sec, 47.263 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670844, 42, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.82, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 38, 931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2771) [M::mem_pestat] mean and std.dev: (199.26, 422.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3691) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1107.024 CPU sec, 65.732 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671256, 49, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 294, 699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1651) [M::mem_pestat] mean and std.dev: (388.67, 272.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2127) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.86, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 38, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 647) [M::mem_pestat] mean and std.dev: (55.05, 74.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 856) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1060.877 CPU sec, 47.170 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671472, 58, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.85, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 41, 395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1147) [M::mem_pestat] mean and std.dev: (99.53, 199.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1523) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1072.830 CPU sec, 62.708 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 670310, 35, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.82, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 34, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 809) [M::mem_pestat] mean and std.dev: (43.15, 56.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1072) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (839, 1316, 1612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3158) [M::mem_pestat] mean and std.dev: (1214.00, 448.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3931) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1110.972 CPU sec, 66.361 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 670876, 41, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (500, 835, 1509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3527) [M::mem_pestat] mean and std.dev: (747.40, 478.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.92, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 33, 59) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 147) [M::mem_pestat] mean and std.dev: (25.70, 17.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 191) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1063.059 CPU sec, 47.127 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 671231, 52, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (458, 801, 1373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3203) [M::mem_pestat] mean and std.dev: (790.08, 446.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4118) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.91, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 33, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 338) [M::mem_pestat] mean and std.dev: (43.77, 62.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1103.580 CPU sec, 65.958 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671287, 53, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.85, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 29, 1804) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5386) [M::mem_pestat] mean and std.dev: (538.73, 1187.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7177) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1060.866 CPU sec, 47.270 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670403, 47, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.91, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 31, 77) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 211) [M::mem_pestat] mean and std.dev: (34.97, 40.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 278) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1111.619 CPU sec, 66.146 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 670870, 39, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (359, 710, 2688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7346) [M::mem_pestat] mean and std.dev: (1351.33, 1169.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9675) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.90, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 42, 99) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 257) [M::mem_pestat] mean and std.dev: (43.84, 40.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1067.504 CPU sec, 47.466 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671304, 55, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (636, 1007, 1902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4434) [M::mem_pestat] mean and std.dev: (1338.00, 1111.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.96, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 37, 1956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5838) [M::mem_pestat] mean and std.dev: (783.54, 1209.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7779) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1089.855 CPU sec, 65.443 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 670895, 47, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 1039, 1323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3685) [M::mem_pestat] mean and std.dev: (1104.60, 949.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4901) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.75, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 58, 1296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3838) [M::mem_pestat] mean and std.dev: (534.79, 1014.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5109) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1047.282 CPU sec, 46.634 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671310, 45, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.81, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 54, 1558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4620) [M::mem_pestat] mean and std.dev: (537.44, 1077.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6151) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1080.727 CPU sec, 65.419 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 670739, 41, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.86, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 49, 1929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5747) [M::mem_pestat] mean and std.dev: (653.72, 1079.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7656) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1051.478 CPU sec, 46.767 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671220, 45, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.89, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 33, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 448) [M::mem_pestat] mean and std.dev: (32.11, 33.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 593) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1102.677 CPU sec, 65.994 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671048, 47, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.76, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 45, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (41.66, 32.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1052.766 CPU sec, 46.837 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671075, 47, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.93, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 48, 1137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3363) [M::mem_pestat] mean and std.dev: (354.07, 629.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4476) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1083.396 CPU sec, 65.712 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670781, 39, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.91, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 40, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (34.61, 29.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 359) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1053.797 CPU sec, 46.928 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670551, 30, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 672) [M::mem_pestat] mean and std.dev: (398.18, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (12, 782) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 49, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1803) [M::mem_pestat] mean and std.dev: (126.68, 248.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2398) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1110.363 CPU sec, 65.719 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670476, 40, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.89, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 29, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (30.44, 28.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 376) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1071.424 CPU sec, 47.696 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671248, 42, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.81, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 39, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 529) [M::mem_pestat] mean and std.dev: (55.21, 76.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 696) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1083.645 CPU sec, 65.255 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671541, 36, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.90, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 54, 716) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2102) [M::mem_pestat] mean and std.dev: (292.39, 549.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2795) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1057.709 CPU sec, 47.035 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670409, 46, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.99, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 44, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 225) [M::mem_pestat] mean and std.dev: (39.30, 32.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 292) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1080.040 CPU sec, 64.659 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670908, 34, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.95, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 42, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1238) [M::mem_pestat] mean and std.dev: (58.12, 92.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1647) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1070.826 CPU sec, 47.576 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670842, 43, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.94, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 51, 1503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4477) [M::mem_pestat] mean and std.dev: (578.17, 997.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5964) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1080.678 CPU sec, 65.672 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670683, 43, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.77, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 50, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1224) [M::mem_pestat] mean and std.dev: (56.85, 76.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1626) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1054.502 CPU sec, 46.954 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670871, 50, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.81, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 34, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (64.73, 100.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1089.930 CPU sec, 65.259 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670910, 39, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.05, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 44, 1767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5245) [M::mem_pestat] mean and std.dev: (520.86, 1067.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6984) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1068.345 CPU sec, 47.389 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 670885, 50, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (518, 804, 1827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4445) [M::mem_pestat] mean and std.dev: (1214.92, 1091.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.09, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 51, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (52.60, 51.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 473) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1092.408 CPU sec, 66.050 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670651, 47, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.08, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 49, 1778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5288) [M::mem_pestat] mean and std.dev: (852.11, 1441.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7043) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1057.206 CPU sec, 47.125 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 670887, 37, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (280, 404, 554) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1102) [M::mem_pestat] mean and std.dev: (324.80, 193.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1376) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.79, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 45, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 198) [M::mem_pestat] mean and std.dev: (38.26, 27.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 257) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1078.592 CPU sec, 67.430 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671311, 34, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.90, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 24, 64) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 166) [M::mem_pestat] mean and std.dev: (28.14, 28.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 217) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1108.227 CPU sec, 65.700 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 669961, 39, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.84, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 42, 608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1780) [M::mem_pestat] mean and std.dev: (129.78, 286.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2366) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (942, 1317, 1662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3102) [M::mem_pestat] mean and std.dev: (1384.55, 775.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4488) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1083.396 CPU sec, 48.405 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 670745, 45, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (445, 1813, 3217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8761) [M::mem_pestat] mean and std.dev: (1554.44, 1413.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11533) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.79, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 39, 1970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5876) [M::mem_pestat] mean and std.dev: (700.14, 1184.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7829) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1101.367 CPU sec, 65.715 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671012, 41, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.97, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 45, 116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 314) [M::mem_pestat] mean and std.dev: (48.71, 52.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 413) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.459 CPU sec, 47.411 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670753, 48, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.94, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 47, 1146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3404) [M::mem_pestat] mean and std.dev: (504.61, 889.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4533) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1084.947 CPU sec, 64.985 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670978, 44, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.86, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 76, 1891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5613) [M::mem_pestat] mean and std.dev: (781.02, 1419.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7474) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1056.192 CPU sec, 46.907 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670848, 56, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.74, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 41, 284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 824) [M::mem_pestat] mean and std.dev: (55.43, 91.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1094) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1092.701 CPU sec, 65.202 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671166, 44, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.88, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 59, 586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1736) [M::mem_pestat] mean and std.dev: (117.25, 243.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2311) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1062.674 CPU sec, 47.251 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670878, 36, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.78, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 41, 910) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2688) [M::mem_pestat] mean and std.dev: (306.03, 614.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3577) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1100.830 CPU sec, 65.670 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671369, 51, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.96, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 37, 89) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 233) [M::mem_pestat] mean and std.dev: (40.79, 39.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 305) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1067.651 CPU sec, 47.503 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 671078, 38, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (310, 1169, 1336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3388) [M::mem_pestat] mean and std.dev: (1032.36, 707.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.84, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 43, 815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2419) [M::mem_pestat] mean and std.dev: (189.61, 498.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3221) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1094.974 CPU sec, 65.428 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670921, 40, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 672) [M::mem_pestat] mean and std.dev: (398.09, 82.87) [M::mem_pestat] low and high boundaries for proper pairs: (12, 782) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 52, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1411) [M::mem_pestat] mean and std.dev: (150.91, 272.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1870) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1071.197 CPU sec, 47.523 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670721, 52, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.09, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 50, 881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2619) [M::mem_pestat] mean and std.dev: (341.15, 613.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3488) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1093.576 CPU sec, 65.515 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670781, 47, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.83, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 30, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 421) [M::mem_pestat] mean and std.dev: (53.33, 77.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1063.015 CPU sec, 47.210 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 670727, 44, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (242, 971, 1473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3935) [M::mem_pestat] mean and std.dev: (833.90, 609.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5166) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.85, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 54, 769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2259) [M::mem_pestat] mean and std.dev: (317.05, 552.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3004) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1105.778 CPU sec, 65.027 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670929, 43, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.02, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 45, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 541) [M::mem_pestat] mean and std.dev: (54.21, 57.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1060.860 CPU sec, 47.074 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671021, 51, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.84, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 31, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (38.05, 35.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1087.692 CPU sec, 65.124 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671191, 37, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.07, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 40, 1531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4549) [M::mem_pestat] mean and std.dev: (288.29, 651.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6058) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (570, 1239, 1368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2964) [M::mem_pestat] mean and std.dev: (996.89, 541.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3762) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1060.732 CPU sec, 47.230 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670684, 56, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.01, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 43, 1174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3480) [M::mem_pestat] mean and std.dev: (376.41, 708.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4633) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1104.664 CPU sec, 65.969 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671070, 51, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.91, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 37, 356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1024) [M::mem_pestat] mean and std.dev: (85.05, 152.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1358) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1073.146 CPU sec, 47.833 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670613, 45, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.90, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 61, 1001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2949) [M::mem_pestat] mean and std.dev: (387.37, 767.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3923) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1102.317 CPU sec, 65.354 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671082, 35, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.13, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 66, 1108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3272) [M::mem_pestat] mean and std.dev: (541.50, 986.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4488) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1061.990 CPU sec, 47.189 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670895, 49, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.80, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 59, 2272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6784) [M::mem_pestat] mean and std.dev: (782.83, 1392.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9040) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1106.365 CPU sec, 65.848 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671288, 58, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.97, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 43, 1099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3265) [M::mem_pestat] mean and std.dev: (357.90, 729.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4348) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1066.723 CPU sec, 47.453 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 670515, 42, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.88, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 58, 1530) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4536) [M::mem_pestat] mean and std.dev: (779.80, 1351.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6188) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1105.901 CPU sec, 66.070 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671012, 34, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.81, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 36, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (40.37, 39.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1069.875 CPU sec, 47.694 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670577, 46, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.94, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 34, 64) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 160) [M::mem_pestat] mean and std.dev: (35.45, 30.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 208) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1101.244 CPU sec, 66.960 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671464, 49, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.00, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 35, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (52.28, 68.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1053) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1056.639 CPU sec, 46.940 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670873, 50, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.84, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 27, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (76.71, 151.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1087.364 CPU sec, 64.932 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671297, 41, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.97, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 34, 704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2066) [M::mem_pestat] mean and std.dev: (111.12, 302.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2747) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.378 CPU sec, 47.402 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670583, 55, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.02, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 40, 388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1132) [M::mem_pestat] mean and std.dev: (76.64, 166.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1504) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (894, 1525, 2980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7152) [M::mem_pestat] mean and std.dev: (1716.00, 1149.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9238) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1083.725 CPU sec, 65.224 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671083, 40, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.84, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 72, 1431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4243) [M::mem_pestat] mean and std.dev: (612.44, 1090.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5649) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1054.043 CPU sec, 47.127 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671178, 61, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.88, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 39, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1590) [M::mem_pestat] mean and std.dev: (110.61, 214.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2114) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1079.164 CPU sec, 65.191 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671126, 53, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.86, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 43, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 932) [M::mem_pestat] mean and std.dev: (78.47, 133.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1237) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1051.367 CPU sec, 46.743 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670854, 43, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.92, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 39, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 210) [M::mem_pestat] mean and std.dev: (37.17, 30.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 275) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1084.104 CPU sec, 65.372 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670557, 46, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.93, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 40, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (64.32, 127.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 815) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (984, 1121, 1544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2664) [M::mem_pestat] mean and std.dev: (1242.30, 484.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3224) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1049.596 CPU sec, 46.764 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671375, 40, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.99, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 43, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 349) [M::mem_pestat] mean and std.dev: (45.47, 46.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 458) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (804, 1070, 1954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4254) [M::mem_pestat] mean and std.dev: (1023.88, 491.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5404) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1075.106 CPU sec, 64.966 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671263, 36, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.88, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 36, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1126) [M::mem_pestat] mean and std.dev: (43.89, 73.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1498) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.375 CPU sec, 46.255 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670997, 37, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.89, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 34, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 214) [M::mem_pestat] mean and std.dev: (42.36, 41.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 281) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1067.422 CPU sec, 64.497 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 670682, 55, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.98, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 27, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 404) [M::mem_pestat] mean and std.dev: (46.04, 63.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 533) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.901 CPU sec, 46.404 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671302, 34, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.01, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 43, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1712) [M::mem_pestat] mean and std.dev: (108.78, 285.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2273) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1090.847 CPU sec, 61.496 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671006, 53, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.00, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (8, 30, 85) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 239) [M::mem_pestat] mean and std.dev: (28.48, 33.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 316) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1081.223 CPU sec, 65.199 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670914, 58, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.74, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 35, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 252) [M::mem_pestat] mean and std.dev: (34.17, 31.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 331) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1047.894 CPU sec, 46.665 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671564, 40, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.99, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 40, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (54.17, 61.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 400) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1068.409 CPU sec, 64.282 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670770, 49, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.88, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 38, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 218) [M::mem_pestat] mean and std.dev: (41.26, 42.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 285) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1048.180 CPU sec, 46.670 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 670862, 35, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (466, 688, 2594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6850) [M::mem_pestat] mean and std.dev: (1791.30, 1977.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.03, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 48, 706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2056) [M::mem_pestat] mean and std.dev: (184.50, 402.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2731) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1067.931 CPU sec, 64.514 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670625, 38, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.82, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 36, 65) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 161) [M::mem_pestat] mean and std.dev: (32.94, 23.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 209) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.292 CPU sec, 46.308 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671223, 45, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.86, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 51, 662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1952) [M::mem_pestat] mean and std.dev: (165.70, 322.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2597) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1067.601 CPU sec, 64.477 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671678, 55, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.94, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 33, 1467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4363) [M::mem_pestat] mean and std.dev: (533.76, 1013.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5811) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.203 CPU sec, 46.122 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670890, 48, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.94, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 46, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1044) [M::mem_pestat] mean and std.dev: (79.74, 124.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1383) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (622, 1046, 1515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3301) [M::mem_pestat] mean and std.dev: (1009.11, 569.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4194) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1071.122 CPU sec, 64.651 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 670883, 41, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 457, 1164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2834) [M::mem_pestat] mean and std.dev: (639.14, 464.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.01, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 45, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (54.59, 57.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.142 CPU sec, 46.335 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671302, 33, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.91, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 31, 1812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5408) [M::mem_pestat] mean and std.dev: (768.03, 1527.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7206) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.006 CPU sec, 64.559 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 670711, 47, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.89, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 37, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 620) [M::mem_pestat] mean and std.dev: (58.61, 106.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 823) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.592 CPU sec, 46.257 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671084, 57, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.98, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 30, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1595) [M::mem_pestat] mean and std.dev: (146.22, 303.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2123) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1067.014 CPU sec, 65.287 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670429, 36, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.80, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 53, 2214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6580) [M::mem_pestat] mean and std.dev: (938.79, 1635.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8763) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.766 CPU sec, 46.121 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671485, 40, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.09, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 41, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (33.35, 29.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 553) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1059.661 CPU sec, 63.542 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671145, 35, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (493, 662, 1529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3601) [M::mem_pestat] mean and std.dev: (809.27, 573.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4637) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.96, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 23, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 178) [M::mem_pestat] mean and std.dev: (21.67, 18.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 233) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1035.019 CPU sec, 46.108 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671107, 48, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.95, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 22, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (25.16, 26.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1060.786 CPU sec, 63.911 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670888, 48, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.92, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 37, 1648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4904) [M::mem_pestat] mean and std.dev: (785.62, 1465.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6649) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1033.790 CPU sec, 45.995 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671185, 65, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.83, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 32, 1411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4195) [M::mem_pestat] mean and std.dev: (485.43, 963.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5587) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.562 CPU sec, 64.347 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 671548, 45, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.78, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 35, 369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1075) [M::mem_pestat] mean and std.dev: (62.17, 126.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1428) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1033.571 CPU sec, 45.944 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671201, 35, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.93, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 31, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 185) [M::mem_pestat] mean and std.dev: (29.68, 21.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 242) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1065.797 CPU sec, 64.031 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671324, 32, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.86, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 49, 1482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4410) [M::mem_pestat] mean and std.dev: (453.66, 800.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5874) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1035.431 CPU sec, 46.061 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671063, 54, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.89, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 40, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 588) [M::mem_pestat] mean and std.dev: (35.05, 32.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 777) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1060.080 CPU sec, 64.271 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 671030, 48, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (366, 804, 1482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3714) [M::mem_pestat] mean and std.dev: (950.67, 730.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 672) [M::mem_pestat] mean and std.dev: (398.13, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (12, 782) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 62, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2280) [M::mem_pestat] mean and std.dev: (158.20, 309.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3030) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1031.767 CPU sec, 45.765 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670998, 45, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.87, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 41, 913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2697) [M::mem_pestat] mean and std.dev: (314.90, 617.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3589) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1057.809 CPU sec, 63.960 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671218, 41, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.94, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 62, 1578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4686) [M::mem_pestat] mean and std.dev: (570.11, 1051.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6240) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1033.497 CPU sec, 46.065 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671471, 53, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.80, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 37, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 993) [M::mem_pestat] mean and std.dev: (86.14, 171.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1319) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1096.968 CPU sec, 65.011 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 670920, 40, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (235, 424, 1409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3757) [M::mem_pestat] mean and std.dev: (934.20, 1000.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4936) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.07, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 37, 2579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7697) [M::mem_pestat] mean and std.dev: (1202.70, 1714.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10256) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (482, 862, 966) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1934) [M::mem_pestat] mean and std.dev: (726.10, 278.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2418) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1082.483 CPU sec, 64.777 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670783, 53, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.80, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 39, 789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2335) [M::mem_pestat] mean and std.dev: (264.94, 531.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3108) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.264 CPU sec, 46.283 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671003, 46, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.82, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 39, 763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2259) [M::mem_pestat] mean and std.dev: (302.61, 598.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3007) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1096.162 CPU sec, 65.708 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671143, 50, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.80, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 31, 95) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 265) [M::mem_pestat] mean and std.dev: (42.47, 45.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 350) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1046.895 CPU sec, 46.684 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 670933, 46, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (579, 950, 1801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4245) [M::mem_pestat] mean and std.dev: (1041.20, 880.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.87, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 61, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 664) [M::mem_pestat] mean and std.dev: (73.00, 97.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 878) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1074.122 CPU sec, 65.293 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671470, 54, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.74, 82.45) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 43, 1080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3200) [M::mem_pestat] mean and std.dev: (363.74, 764.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4260) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1044.141 CPU sec, 46.325 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671185, 53, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 370, 1621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4301) [M::mem_pestat] mean and std.dev: (958.40, 979.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5641) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.85, 82.46) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 32, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (42.27, 56.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1081.592 CPU sec, 65.126 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670884, 40, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.97, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 53, 1307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3873) [M::mem_pestat] mean and std.dev: (644.00, 1150.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5246) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1055.430 CPU sec, 46.931 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670886, 47, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.84, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 51, 2435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7261) [M::mem_pestat] mean and std.dev: (958.00, 1563.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9674) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1042, 1264, 1838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3430) [M::mem_pestat] mean and std.dev: (1230.10, 485.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4226) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1082.021 CPU sec, 65.115 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671552, 51, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.98, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 32, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 172) [M::mem_pestat] mean and std.dev: (30.61, 23.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 224) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1049.812 CPU sec, 46.648 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 670470, 38, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (331, 368, 1152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2794) [M::mem_pestat] mean and std.dev: (653.09, 633.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3615) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.81, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 58, 1525) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4537) [M::mem_pestat] mean and std.dev: (655.26, 1225.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6043) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1083.638 CPU sec, 65.123 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671480, 44, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.99, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 66, 1338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3968) [M::mem_pestat] mean and std.dev: (438.21, 829.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5283) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1044.500 CPU sec, 46.464 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671008, 43, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.00, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 43, 2622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7832) [M::mem_pestat] mean and std.dev: (981.66, 1798.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10437) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1073.830 CPU sec, 64.608 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670946, 48, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.81, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 37, 1130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3360) [M::mem_pestat] mean and std.dev: (376.44, 831.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4475) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1048.370 CPU sec, 46.653 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671429, 41, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.86, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 45, 687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2011) [M::mem_pestat] mean and std.dev: (217.57, 426.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2673) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1071.176 CPU sec, 64.020 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670313, 46, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.98, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 33, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (45.65, 59.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.600 CPU sec, 46.429 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671024, 52, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.96, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 65, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1165) [M::mem_pestat] mean and std.dev: (133.36, 242.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1545) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1068.314 CPU sec, 64.481 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671061, 42, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.96, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 37, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 736) [M::mem_pestat] mean and std.dev: (45.75, 55.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 976) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.476 CPU sec, 46.237 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 670870, 54, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 783, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1852) [M::mem_pestat] mean and std.dev: (635.60, 360.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.08, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 33, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 254) [M::mem_pestat] mean and std.dev: (32.81, 36.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1074.813 CPU sec, 64.942 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670649, 42, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.12, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 37, 252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 728) [M::mem_pestat] mean and std.dev: (77.65, 162.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1047.102 CPU sec, 46.506 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671502, 43, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.93, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 28, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 419) [M::mem_pestat] mean and std.dev: (44.42, 55.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 554) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1067.218 CPU sec, 65.305 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671374, 50, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.83, 82.41) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 29, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 491) [M::mem_pestat] mean and std.dev: (41.08, 60.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 651) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1035.290 CPU sec, 46.028 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671087, 43, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.98, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 43, 1602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4762) [M::mem_pestat] mean and std.dev: (607.35, 1257.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6342) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1060.825 CPU sec, 64.254 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 670780, 49, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (392, 950, 1320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3176) [M::mem_pestat] mean and std.dev: (880.67, 653.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4104) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.06, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 56, 1278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3786) [M::mem_pestat] mean and std.dev: (312.24, 621.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5040) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.827 CPU sec, 46.295 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670479, 31, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.76, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 32, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 178) [M::mem_pestat] mean and std.dev: (33.35, 33.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 233) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1080.331 CPU sec, 65.453 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671559, 44, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.78, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 34, 217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 611) [M::mem_pestat] mean and std.dev: (55.58, 72.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 808) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.652 CPU sec, 46.146 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671158, 50, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (272, 425, 818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1910) [M::mem_pestat] mean and std.dev: (489.67, 316.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.87, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 46, 715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2105) [M::mem_pestat] mean and std.dev: (210.52, 449.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2800) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1110.575 CPU sec, 68.860 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671531, 46, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (352, 736, 1389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3463) [M::mem_pestat] mean and std.dev: (755.30, 532.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4500) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.95, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 46, 1210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3588) [M::mem_pestat] mean and std.dev: (372.50, 698.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4777) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1088.244 CPU sec, 65.087 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670519, 41, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.90, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 54, 2926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8732) [M::mem_pestat] mean and std.dev: (1303.62, 2153.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11635) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1061.347 CPU sec, 47.383 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 670390, 42, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (70, 425, 928) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2644) [M::mem_pestat] mean and std.dev: (528.00, 631.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.84, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 39, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1082) [M::mem_pestat] mean and std.dev: (45.34, 65.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1435) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1082.501 CPU sec, 65.797 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671337, 44, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.86, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (6, 19, 52) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 144) [M::mem_pestat] mean and std.dev: (24.29, 28.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 190) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.466 CPU sec, 47.305 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670771, 48, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.03, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 60, 1568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4648) [M::mem_pestat] mean and std.dev: (795.28, 1379.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6312) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1093.677 CPU sec, 66.286 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671322, 40, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.98, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 47, 239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 669) [M::mem_pestat] mean and std.dev: (71.61, 88.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1056.946 CPU sec, 46.960 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671347, 46, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 672) [M::mem_pestat] mean and std.dev: (398.12, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (12, 782) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 33, 56) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 130) [M::mem_pestat] mean and std.dev: (30.70, 22.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 167) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1092.747 CPU sec, 65.567 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670995, 34, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.94, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 44, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 506) [M::mem_pestat] mean and std.dev: (75.41, 109.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 670) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1048.091 CPU sec, 46.568 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671415, 48, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.00, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 46, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (74.78, 108.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 816) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (879, 1082, 1520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2802) [M::mem_pestat] mean and std.dev: (906.80, 431.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3443) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1087.427 CPU sec, 66.047 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670609, 49, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.89, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 53, 533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1561) [M::mem_pestat] mean and std.dev: (195.00, 363.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2075) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1054.601 CPU sec, 46.874 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670928, 46, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.06, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 43, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1474) [M::mem_pestat] mean and std.dev: (58.97, 98.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1960) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1088.542 CPU sec, 65.639 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670546, 28, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.93, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 73, 1050) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3096) [M::mem_pestat] mean and std.dev: (236.79, 444.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4119) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1048.577 CPU sec, 46.563 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670053, 45, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.90, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 58, 976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2886) [M::mem_pestat] mean and std.dev: (238.82, 575.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3841) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1097.026 CPU sec, 66.030 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 670858, 52, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.83, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 42, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (44.34, 42.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1056.400 CPU sec, 46.958 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670864, 43, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.90, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 33, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (61.41, 94.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (602, 1450, 1586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3554) [M::mem_pestat] mean and std.dev: (1418.40, 922.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5109) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1095.792 CPU sec, 65.546 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671509, 46, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.95, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 43, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (47.65, 51.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1049.406 CPU sec, 46.634 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671741, 47, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.95, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 51, 1082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3214) [M::mem_pestat] mean and std.dev: (446.77, 854.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4280) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1112.356 CPU sec, 66.110 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670965, 47, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.86, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 37, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 530) [M::mem_pestat] mean and std.dev: (72.60, 116.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 701) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1057.640 CPU sec, 47.160 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670812, 54, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.87, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 50, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1426) [M::mem_pestat] mean and std.dev: (103.45, 215.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1896) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1107.034 CPU sec, 65.759 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671054, 39, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.85, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 39, 889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2635) [M::mem_pestat] mean and std.dev: (176.75, 481.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3508) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.410 CPU sec, 47.388 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671445, 59, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.91, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 46, 1402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4178) [M::mem_pestat] mean and std.dev: (466.38, 922.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5566) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1109.463 CPU sec, 66.013 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670712, 45, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.93, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 41, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (41.00, 44.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 460) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.816 CPU sec, 47.380 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670325, 52, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.93, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 39, 601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1763) [M::mem_pestat] mean and std.dev: (199.22, 395.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2344) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1107.681 CPU sec, 66.133 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670663, 45, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.87, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 30, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 353) [M::mem_pestat] mean and std.dev: (41.27, 41.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 465) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1063.366 CPU sec, 47.338 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670455, 47, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.12, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 34, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (47.50, 70.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1111.625 CPU sec, 66.380 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670593, 30, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.83, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 49, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1535) [M::mem_pestat] mean and std.dev: (172.80, 300.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2042) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1062.271 CPU sec, 47.225 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671058, 45, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.83, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 63, 1596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4736) [M::mem_pestat] mean and std.dev: (830.67, 1307.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6306) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1099.973 CPU sec, 66.413 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671151, 40, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.00, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 107, 1814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5408) [M::mem_pestat] mean and std.dev: (864.08, 1269.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7205) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.062 CPU sec, 47.298 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670986, 39, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.93, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 33, 100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 278) [M::mem_pestat] mean and std.dev: (39.75, 45.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 367) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1087.499 CPU sec, 66.261 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 670585, 39, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.98, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 37, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 221) [M::mem_pestat] mean and std.dev: (42.45, 47.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 290) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1071.239 CPU sec, 47.579 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670595, 45, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.93, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 55, 2403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7163) [M::mem_pestat] mean and std.dev: (1029.77, 1704.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9543) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1108.419 CPU sec, 66.203 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670594, 34, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.92, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 46, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1796) [M::mem_pestat] mean and std.dev: (210.50, 408.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2389) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1067.456 CPU sec, 47.500 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670291, 43, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 672) [M::mem_pestat] mean and std.dev: (398.18, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (12, 782) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 65, 443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1273) [M::mem_pestat] mean and std.dev: (75.94, 103.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1688) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1097.423 CPU sec, 66.509 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670912, 50, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.82, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 47, 981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2911) [M::mem_pestat] mean and std.dev: (196.32, 389.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3876) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1067.021 CPU sec, 47.361 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 670763, 48, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.89, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 58, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2080) [M::mem_pestat] mean and std.dev: (290.77, 543.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2764) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1111.069 CPU sec, 66.061 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671560, 43, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.81, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 51, 1535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4547) [M::mem_pestat] mean and std.dev: (758.07, 1330.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6081) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1080.750 CPU sec, 48.059 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671272, 42, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.91, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 34, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 315) [M::mem_pestat] mean and std.dev: (49.72, 55.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 415) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (450, 2071, 2866) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7698) [M::mem_pestat] mean and std.dev: (1630.90, 1109.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10114) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1101.778 CPU sec, 65.827 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671210, 50, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (342, 1620, 2006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5334) [M::mem_pestat] mean and std.dev: (1410.70, 894.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6998) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.77, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 38, 1895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5647) [M::mem_pestat] mean and std.dev: (484.48, 1055.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7523) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1072.436 CPU sec, 47.683 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670804, 60, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.97, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 49, 1380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4110) [M::mem_pestat] mean and std.dev: (377.04, 729.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5475) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1107.484 CPU sec, 65.806 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 670892, 49, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.72, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 36, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (33.37, 32.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 523) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1069.361 CPU sec, 47.697 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671061, 38, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 316, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1521) [M::mem_pestat] mean and std.dev: (350.78, 245.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1967) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.90, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 36, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (46.63, 67.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1094.992 CPU sec, 62.231 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671173, 38, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.94, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 35, 52) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 134) [M::mem_pestat] mean and std.dev: (30.18, 24.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 175) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1089.088 CPU sec, 65.823 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670858, 38, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.76, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 28, 57) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 141) [M::mem_pestat] mean and std.dev: (25.33, 19.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 183) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1067.961 CPU sec, 47.482 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671200, 46, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.95, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 47, 1735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5163) [M::mem_pestat] mean and std.dev: (608.00, 1094.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6877) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1097.183 CPU sec, 65.420 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 670826, 41, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (368, 474, 1135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2669) [M::mem_pestat] mean and std.dev: (523.88, 271.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3436) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.88, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 35, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 386) [M::mem_pestat] mean and std.dev: (39.12, 37.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 507) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1059.232 CPU sec, 47.108 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671414, 33, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 421, 2141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5743) [M::mem_pestat] mean and std.dev: (1026.30, 896.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.84, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 46, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1152) [M::mem_pestat] mean and std.dev: (140.38, 287.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1531) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1075.182 CPU sec, 65.257 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670547, 41, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.89, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 37, 693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2047) [M::mem_pestat] mean and std.dev: (103.45, 209.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2724) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1058.823 CPU sec, 47.219 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670913, 48, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.82, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 48, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 262) [M::mem_pestat] mean and std.dev: (45.41, 47.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 344) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1102.750 CPU sec, 65.939 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 670193, 49, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 231, 647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1649) [M::mem_pestat] mean and std.dev: (257.83, 197.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2150) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.94, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 43, 742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2196) [M::mem_pestat] mean and std.dev: (210.40, 497.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2923) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1068.244 CPU sec, 47.574 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671122, 47, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.83, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 36, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (40.18, 47.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1101.251 CPU sec, 65.817 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670437, 48, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.05, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 48, 1650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4904) [M::mem_pestat] mean and std.dev: (568.40, 1188.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6531) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1072.223 CPU sec, 47.656 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671377, 36, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.80, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 32, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 186) [M::mem_pestat] mean and std.dev: (32.17, 23.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 243) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1092.835 CPU sec, 66.008 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670952, 36, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.90, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 28, 92) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 250) [M::mem_pestat] mean and std.dev: (26.46, 23.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 329) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1065.272 CPU sec, 47.424 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670949, 50, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.06, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 81) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 213) [M::mem_pestat] mean and std.dev: (29.41, 24.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 279) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1107.488 CPU sec, 66.419 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671064, 39, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.90, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 39, 99) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251) [M::mem_pestat] mean and std.dev: (32.53, 22.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1065.983 CPU sec, 47.386 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 670597, 47, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (321, 756, 1238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3072) [M::mem_pestat] mean and std.dev: (691.44, 445.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3989) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.85, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 40, 1747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5195) [M::mem_pestat] mean and std.dev: (548.71, 1007.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6919) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1106.630 CPU sec, 65.803 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670444, 39, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.77, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 50, 1648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4896) [M::mem_pestat] mean and std.dev: (767.30, 1269.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6520) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1061.573 CPU sec, 47.129 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 670823, 38, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (403, 429, 1172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2710) [M::mem_pestat] mean and std.dev: (641.40, 361.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.88, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 46, 1129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3341) [M::mem_pestat] mean and std.dev: (413.50, 659.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4447) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1100.606 CPU sec, 65.473 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671168, 41, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (440, 1008, 1833) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4619) [M::mem_pestat] mean and std.dev: (992.78, 1028.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6012) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.90, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 47, 898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2646) [M::mem_pestat] mean and std.dev: (297.39, 687.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3520) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1068.955 CPU sec, 47.544 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670846, 41, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.97, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 54, 1170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3480) [M::mem_pestat] mean and std.dev: (591.75, 1064.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4850) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1107.253 CPU sec, 66.246 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671049, 39, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.86, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 36, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 395) [M::mem_pestat] mean and std.dev: (44.84, 53.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1063.261 CPU sec, 47.206 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670873, 35, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.98, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 41, 1594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4736) [M::mem_pestat] mean and std.dev: (548.68, 996.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6307) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1097.745 CPU sec, 65.465 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671213, 53, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.02, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 46, 612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1790) [M::mem_pestat] mean and std.dev: (150.66, 348.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2379) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1069.820 CPU sec, 47.749 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670621, 39, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.91, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 45, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1803) [M::mem_pestat] mean and std.dev: (202.47, 390.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2398) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1112.120 CPU sec, 66.227 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671289, 48, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.84, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 38, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (47.97, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1066.545 CPU sec, 47.413 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 670489, 39, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (416, 662, 1852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4724) [M::mem_pestat] mean and std.dev: (884.64, 727.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6160) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.74, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 49, 1476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4378) [M::mem_pestat] mean and std.dev: (593.35, 1057.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5829) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1102.466 CPU sec, 65.588 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671199, 43, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.95, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 51, 282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 812) [M::mem_pestat] mean and std.dev: (62.88, 83.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1077) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1072.431 CPU sec, 47.735 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671043, 40, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.96, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 52, 1268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3734) [M::mem_pestat] mean and std.dev: (359.97, 758.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4967) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1101.564 CPU sec, 66.005 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671817, 49, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.72, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 30, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1256) [M::mem_pestat] mean and std.dev: (146.26, 252.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1669) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1092.563 CPU sec, 66.086 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671349, 53, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.90, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 33, 97) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 253) [M::mem_pestat] mean and std.dev: (42.12, 45.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 331) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1066.863 CPU sec, 47.470 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671104, 45, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.91, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 48, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1490) [M::mem_pestat] mean and std.dev: (132.57, 291.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1981) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1100.199 CPU sec, 65.357 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670856, 32, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.81, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 37, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (47.35, 51.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1057.322 CPU sec, 47.110 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671282, 49, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.93, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 29, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (44.77, 49.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 374) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1099.725 CPU sec, 65.316 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671259, 49, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.79, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 72, 1125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3329) [M::mem_pestat] mean and std.dev: (398.36, 749.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4431) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1070.845 CPU sec, 47.823 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671163, 41, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.00, 82.85) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 38, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1150) [M::mem_pestat] mean and std.dev: (90.45, 169.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1526) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1084.578 CPU sec, 65.841 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671437, 42, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.95, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 40, 497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1451) [M::mem_pestat] mean and std.dev: (140.94, 329.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1928) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1066.596 CPU sec, 47.545 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671270, 39, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.08, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 32, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 463) [M::mem_pestat] mean and std.dev: (48.38, 59.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1104.958 CPU sec, 66.789 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670883, 44, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.03, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 86, 2064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6120) [M::mem_pestat] mean and std.dev: (795.95, 1192.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8148) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.423 CPU sec, 47.320 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670736, 56, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.85, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 64, 718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2102) [M::mem_pestat] mean and std.dev: (196.91, 401.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2794) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1087.780 CPU sec, 65.004 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670428, 41, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.98, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 36, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 246) [M::mem_pestat] mean and std.dev: (37.65, 32.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 322) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.704 CPU sec, 47.368 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671567, 45, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 602, 1041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2513) [M::mem_pestat] mean and std.dev: (687.91, 559.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3249) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.88, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 55, 387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1133) [M::mem_pestat] mean and std.dev: (58.23, 76.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1506) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1104.682 CPU sec, 65.561 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670955, 45, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.82, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 49, 1948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5816) [M::mem_pestat] mean and std.dev: (561.64, 1099.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7750) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1066.762 CPU sec, 47.531 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671014, 45, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.87, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 42, 2320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6918) [M::mem_pestat] mean and std.dev: (1011.50, 1800.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9217) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (988, 1535, 2359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5101) [M::mem_pestat] mean and std.dev: (1398.18, 647.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6472) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1107.082 CPU sec, 65.807 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671019, 38, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.00, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 44, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (44.31, 41.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (828, 1191, 1589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3111) [M::mem_pestat] mean and std.dev: (1259.80, 755.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4282) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1059.806 CPU sec, 47.126 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 670622, 56, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 409, 572) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1280) [M::mem_pestat] mean and std.dev: (390.44, 233.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.83, 82.41) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 31, 728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2156) [M::mem_pestat] mean and std.dev: (201.75, 457.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2870) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1100.141 CPU sec, 65.452 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671153, 29, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (249, 605, 1978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5436) [M::mem_pestat] mean and std.dev: (1175.20, 1222.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7165) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.92, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 43, 384) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1098) [M::mem_pestat] mean and std.dev: (74.96, 97.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1455) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1068.577 CPU sec, 47.472 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671466, 56, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.00, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 33, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 262) [M::mem_pestat] mean and std.dev: (32.82, 33.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 345) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1096.795 CPU sec, 65.323 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671269, 48, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.84, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 43, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (57.75, 72.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.451 CPU sec, 47.340 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 670889, 44, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 672) [M::mem_pestat] mean and std.dev: (398.15, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (12, 782) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 52, 1553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4617) [M::mem_pestat] mean and std.dev: (607.46, 1092.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6149) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1095.089 CPU sec, 66.369 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671246, 42, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.91, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 32, 52) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 128) [M::mem_pestat] mean and std.dev: (25.50, 18.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 166) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1065.403 CPU sec, 47.371 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670651, 54, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.03, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 46, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1004) [M::mem_pestat] mean and std.dev: (92.33, 137.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1332) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1104.685 CPU sec, 66.200 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670960, 36, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.94, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 32, 611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1791) [M::mem_pestat] mean and std.dev: (159.53, 385.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2381) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (451, 863, 1876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4726) [M::mem_pestat] mean and std.dev: (1115.10, 833.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6151) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1062.740 CPU sec, 47.206 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671116, 39, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.93, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 30, 1378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4112) [M::mem_pestat] mean and std.dev: (428.89, 860.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5479) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1105.623 CPU sec, 65.704 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670857, 35, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.96, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 30, 1185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3525) [M::mem_pestat] mean and std.dev: (270.07, 552.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4695) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1061.317 CPU sec, 47.281 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670661, 59, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.92, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 35, 879) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2609) [M::mem_pestat] mean and std.dev: (284.50, 579.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3474) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1110.773 CPU sec, 69.460 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 670343, 45, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (352, 496, 1459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3673) [M::mem_pestat] mean and std.dev: (807.00, 859.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.00, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 34, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (43.87, 46.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1109.148 CPU sec, 66.154 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670998, 39, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.00, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 41, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 476) [M::mem_pestat] mean and std.dev: (47.48, 54.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 629) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1077.493 CPU sec, 47.818 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671276, 46, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (313, 822, 1152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2830) [M::mem_pestat] mean and std.dev: (758.10, 477.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.09, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 41, 81) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 221) [M::mem_pestat] mean and std.dev: (38.87, 36.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 291) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1109.450 CPU sec, 65.867 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 670922, 50, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.84, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 50, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (44.34, 57.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1066.183 CPU sec, 47.325 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 670893, 53, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.96, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (51.09, 72.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 563) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1103.561 CPU sec, 65.939 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670702, 40, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.96, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 39, 1013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3011) [M::mem_pestat] mean and std.dev: (418.86, 800.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4010) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.494 CPU sec, 47.569 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671101, 44, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.93, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 46, 1282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3800) [M::mem_pestat] mean and std.dev: (299.18, 560.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5059) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1099.191 CPU sec, 65.679 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670914, 42, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 672) [M::mem_pestat] mean and std.dev: (398.07, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (12, 782) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 48, 875) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2569) [M::mem_pestat] mean and std.dev: (273.58, 568.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3416) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1073.619 CPU sec, 47.929 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 670775, 38, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (288, 338, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2763) [M::mem_pestat] mean and std.dev: (501.56, 380.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3588) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.87, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 65, 1887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5621) [M::mem_pestat] mean and std.dev: (494.67, 1102.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7488) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1088.771 CPU sec, 66.020 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670859, 51, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.85, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 43, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (57.66, 62.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 710) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1067.883 CPU sec, 47.564 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671121, 43, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.72, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 68, 1691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5033) [M::mem_pestat] mean and std.dev: (777.34, 1356.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6704) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1092.369 CPU sec, 66.132 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671200, 58, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.96, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 29, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (37.55, 45.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 371) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1068.431 CPU sec, 47.594 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670576, 50, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.82, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 37, 269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 775) [M::mem_pestat] mean and std.dev: (44.50, 58.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1028) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1106.076 CPU sec, 65.980 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 670877, 43, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 672) [M::mem_pestat] mean and std.dev: (398.13, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (12, 782) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 64, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (59.86, 51.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 545) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1065.018 CPU sec, 47.333 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 670932, 55, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.03, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 49, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 875) [M::mem_pestat] mean and std.dev: (76.30, 114.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1159) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1107.461 CPU sec, 65.498 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671262, 46, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.82, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 57, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1173) [M::mem_pestat] mean and std.dev: (94.43, 164.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1556) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.775 CPU sec, 47.290 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671118, 48, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.80, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 34, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (51.49, 83.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1011) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1105.274 CPU sec, 66.398 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 670978, 42, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (246, 609, 936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2316) [M::mem_pestat] mean and std.dev: (591.00, 370.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3006) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.82, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 38, 60) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 132) [M::mem_pestat] mean and std.dev: (32.09, 19.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 168) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1070.641 CPU sec, 47.660 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671051, 46, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.82, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 46, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 825) [M::mem_pestat] mean and std.dev: (80.95, 115.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1093.520 CPU sec, 66.397 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670932, 51, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.89, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 49, 2188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6530) [M::mem_pestat] mean and std.dev: (776.64, 1575.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8701) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (699, 1386, 1930) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4392) [M::mem_pestat] mean and std.dev: (1303.00, 851.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5623) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1066.545 CPU sec, 47.500 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670548, 43, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.89, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 29, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 254) [M::mem_pestat] mean and std.dev: (38.11, 46.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1101.511 CPU sec, 65.846 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 670897, 39, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.88, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 36, 70) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 184) [M::mem_pestat] mean and std.dev: (36.38, 35.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 241) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1070.496 CPU sec, 47.856 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 670650, 46, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (190, 285, 1158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3094) [M::mem_pestat] mean and std.dev: (320.11, 331.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4062) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.81, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 55, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (75.11, 115.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1091.291 CPU sec, 65.594 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670680, 46, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.94, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 44, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (41.69, 45.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 835) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1075.691 CPU sec, 47.904 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671388, 45, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.85, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 31, 1587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4725) [M::mem_pestat] mean and std.dev: (477.60, 990.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6294) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1091.932 CPU sec, 66.519 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 670352, 41, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (440, 498, 1798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4514) [M::mem_pestat] mean and std.dev: (891.58, 677.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5872) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.87, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 48, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (48.53, 41.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1063.406 CPU sec, 47.208 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671272, 45, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.77, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 53, 921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2735) [M::mem_pestat] mean and std.dev: (271.23, 510.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3642) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1104.073 CPU sec, 65.872 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670719, 47, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.03, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 71, 874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2582) [M::mem_pestat] mean and std.dev: (235.10, 514.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3436) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1061.980 CPU sec, 47.288 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671141, 45, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.03, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 39, 968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2862) [M::mem_pestat] mean and std.dev: (260.36, 572.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3809) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1077.633 CPU sec, 64.900 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670573, 49, 14) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.89, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 51, 1200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3556) [M::mem_pestat] mean and std.dev: (446.11, 869.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4734) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (384, 1069, 1396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3420) [M::mem_pestat] mean and std.dev: (993.50, 635.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4432) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1067.404 CPU sec, 47.481 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671263, 40, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.05, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 32, 941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2783) [M::mem_pestat] mean and std.dev: (265.86, 546.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3704) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1087.458 CPU sec, 65.845 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670996, 36, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.98, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 44, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 731) [M::mem_pestat] mean and std.dev: (48.68, 52.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (310, 659, 1275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3205) [M::mem_pestat] mean and std.dev: (763.27, 552.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4170) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1069.622 CPU sec, 47.627 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670981, 52, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.97, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 44, 1148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3416) [M::mem_pestat] mean and std.dev: (453.17, 829.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4550) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1102.951 CPU sec, 65.450 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671020, 44, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.84, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 49, 690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2010) [M::mem_pestat] mean and std.dev: (205.49, 367.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2670) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1067.664 CPU sec, 47.429 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670434, 50, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.86, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 39, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (44.27, 60.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1102.992 CPU sec, 65.898 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671438, 59, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.96, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 84, 1691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5037) [M::mem_pestat] mean and std.dev: (685.48, 1145.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6710) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.097 CPU sec, 47.293 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670560, 29, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.85, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 33, 801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2375) [M::mem_pestat] mean and std.dev: (317.37, 567.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3162) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1097.194 CPU sec, 65.646 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 670825, 43, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (237, 886, 1686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4584) [M::mem_pestat] mean and std.dev: (684.62, 589.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6033) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.85, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 30, 1424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4224) [M::mem_pestat] mean and std.dev: (411.15, 801.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5624) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1059.794 CPU sec, 47.586 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670585, 44, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.98, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 35, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (41.11, 49.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1092.625 CPU sec, 65.833 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671289, 47, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (331, 822, 1220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2998) [M::mem_pestat] mean and std.dev: (722.44, 543.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3887) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.96, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 38, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 643) [M::mem_pestat] mean and std.dev: (68.71, 117.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1060.352 CPU sec, 47.212 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671412, 54, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.75, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 35, 195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 549) [M::mem_pestat] mean and std.dev: (54.63, 90.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 726) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1079.436 CPU sec, 62.346 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670842, 46, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.86, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 29, 101) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (40.41, 47.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 374) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (989, 1186, 1850) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3572) [M::mem_pestat] mean and std.dev: (1152.44, 541.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4433) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1104.282 CPU sec, 65.876 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671487, 39, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (387, 677, 1584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3978) [M::mem_pestat] mean and std.dev: (831.20, 552.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5175) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.99, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 37, 81) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 191) [M::mem_pestat] mean and std.dev: (39.16, 33.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 246) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1062.711 CPU sec, 47.206 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671081, 53, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.88, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 37, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (46.40, 48.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 361) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1105.290 CPU sec, 65.680 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 671760, 46, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.95, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 43, 1142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3376) [M::mem_pestat] mean and std.dev: (267.67, 575.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4493) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1062.008 CPU sec, 47.177 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 671106, 41, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (348, 530, 1401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3507) [M::mem_pestat] mean and std.dev: (815.50, 710.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4560) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.04, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 43, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1824) [M::mem_pestat] mean and std.dev: (202.00, 446.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2427) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1104.340 CPU sec, 65.921 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671373, 46, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.92, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 39, 637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1877) [M::mem_pestat] mean and std.dev: (113.95, 278.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2497) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1061.649 CPU sec, 47.163 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670617, 39, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.93, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 32, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 169) [M::mem_pestat] mean and std.dev: (35.82, 30.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 218) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1093.930 CPU sec, 66.734 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671102, 41, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.83, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 37, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 175) [M::mem_pestat] mean and std.dev: (40.81, 35.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 227) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1067.502 CPU sec, 47.631 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670985, 45, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.71, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 55, 326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942) [M::mem_pestat] mean and std.dev: (105.61, 171.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1250) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1102.596 CPU sec, 65.888 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670954, 30, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.82, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 50, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 435) [M::mem_pestat] mean and std.dev: (60.58, 73.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1062.537 CPU sec, 47.189 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671515, 48, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.94, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 28, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (73.90, 130.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1150) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1104.510 CPU sec, 66.064 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671118, 40, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.00, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 80, 1963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5849) [M::mem_pestat] mean and std.dev: (787.22, 1336.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7792) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1057.958 CPU sec, 46.960 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670326, 47, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.95, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 30, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (33.65, 31.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1113.186 CPU sec, 66.767 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 670786, 45, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (441, 912, 1508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3642) [M::mem_pestat] mean and std.dev: (948.00, 626.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4709) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.84, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 45, 2294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6850) [M::mem_pestat] mean and std.dev: (700.37, 1420.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9128) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1065.567 CPU sec, 47.361 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671361, 48, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.90, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 65, 511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1499) [M::mem_pestat] mean and std.dev: (118.62, 258.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1993) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1101.019 CPU sec, 65.371 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671079, 53, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.86, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 33, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 929) [M::mem_pestat] mean and std.dev: (66.45, 117.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1234) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1063.928 CPU sec, 47.347 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671253, 39, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.93, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 48, 526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1534) [M::mem_pestat] mean and std.dev: (144.50, 303.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2038) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1099.606 CPU sec, 65.252 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671585, 45, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.92, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 52, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1486) [M::mem_pestat] mean and std.dev: (101.17, 165.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1975) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1053.663 CPU sec, 46.997 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 670446, 42, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.87, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 47, 1200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3570) [M::mem_pestat] mean and std.dev: (335.44, 755.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4755) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1101.470 CPU sec, 65.261 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671216, 42, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.04, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 51, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 674) [M::mem_pestat] mean and std.dev: (78.32, 132.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 893) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1061.393 CPU sec, 47.189 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670515, 41, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.72, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 35, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 568) [M::mem_pestat] mean and std.dev: (48.03, 91.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 753) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1106.818 CPU sec, 66.116 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 670989, 40, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 295, 561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1193) [M::mem_pestat] mean and std.dev: (320.67, 157.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1509) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.87, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 35, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (53.72, 76.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1061.018 CPU sec, 47.244 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671107, 43, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.83, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 46, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 579) [M::mem_pestat] mean and std.dev: (47.36, 44.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 764) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1087.933 CPU sec, 66.029 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 670992, 52, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.87, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 44, 935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2761) [M::mem_pestat] mean and std.dev: (219.26, 557.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3674) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1058.523 CPU sec, 47.168 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671537, 38, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (294, 391, 582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1158) [M::mem_pestat] mean and std.dev: (395.89, 141.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.79, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 31, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (37.87, 54.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1091.005 CPU sec, 66.242 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671055, 44, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.84, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 48, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 499) [M::mem_pestat] mean and std.dev: (59.69, 83.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1066.369 CPU sec, 47.442 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671377, 46, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.81, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 38, 1867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5577) [M::mem_pestat] mean and std.dev: (519.37, 1048.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7432) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1082.421 CPU sec, 66.057 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670365, 44, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 672) [M::mem_pestat] mean and std.dev: (398.18, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (12, 782) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 33, 341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1005) [M::mem_pestat] mean and std.dev: (58.56, 88.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1337) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1101.274 CPU sec, 65.796 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670729, 37, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.01, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 34, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 185) [M::mem_pestat] mean and std.dev: (34.56, 31.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 242) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1066.548 CPU sec, 47.372 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670708, 36, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.04, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 47, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (45.57, 40.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 802) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1105.355 CPU sec, 66.271 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671488, 39, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.99, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 56, 635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1865) [M::mem_pestat] mean and std.dev: (193.70, 378.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2480) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1061.985 CPU sec, 47.197 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670650, 44, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.88, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 38, 97) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 251) [M::mem_pestat] mean and std.dev: (40.51, 33.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 328) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (732, 1340, 2874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7158) [M::mem_pestat] mean and std.dev: (1712.45, 1024.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9300) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1096.054 CPU sec, 66.121 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671650, 38, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.83, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 63, 1953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5797) [M::mem_pestat] mean and std.dev: (670.12, 1048.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7719) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1072.989 CPU sec, 47.743 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670956, 52, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.79, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 52, 1292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3838) [M::mem_pestat] mean and std.dev: (432.40, 834.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5111) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1101.973 CPU sec, 65.459 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670765, 42, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.01, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 45, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 623) [M::mem_pestat] mean and std.dev: (49.55, 61.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1068.189 CPU sec, 47.636 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671157, 40, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.93, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 42, 1504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4470) [M::mem_pestat] mean and std.dev: (685.76, 1300.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5953) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1098.010 CPU sec, 66.042 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671281, 55, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.87, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 63, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 548) [M::mem_pestat] mean and std.dev: (66.80, 86.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (696, 1139, 1265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2403) [M::mem_pestat] mean and std.dev: (939.50, 399.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2972) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1064.944 CPU sec, 47.410 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 670586, 52, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.93, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 49, 1687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4997) [M::mem_pestat] mean and std.dev: (834.18, 1413.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6652) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1107.300 CPU sec, 65.954 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670695, 42, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 672) [M::mem_pestat] mean and std.dev: (398.10, 82.87) [M::mem_pestat] low and high boundaries for proper pairs: (12, 782) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 24, 42) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 102) [M::mem_pestat] mean and std.dev: (23.69, 18.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 132) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1072.938 CPU sec, 47.654 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670827, 45, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.94, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 51, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1416) [M::mem_pestat] mean and std.dev: (73.54, 106.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1882) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1104.153 CPU sec, 65.504 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671257, 52, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1479, 1717, 1747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (943, 2283) [M::mem_pestat] mean and std.dev: (1641.33, 105.02) [M::mem_pestat] low and high boundaries for proper pairs: (675, 2551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.99, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 34, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 296) [M::mem_pestat] mean and std.dev: (35.38, 35.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 391) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1059.756 CPU sec, 47.115 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 670870, 45, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 334, 348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (278.56, 121.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 795) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.94, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 51, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (69.78, 95.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1153) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1106.008 CPU sec, 65.778 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670977, 51, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.01, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 58, 944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2796) [M::mem_pestat] mean and std.dev: (271.52, 495.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3722) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1059.514 CPU sec, 47.126 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670859, 40, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.83, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 43, 1009) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2981) [M::mem_pestat] mean and std.dev: (290.94, 723.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3967) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1090.092 CPU sec, 65.350 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 671039, 38, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (350, 743, 1624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4172) [M::mem_pestat] mean and std.dev: (691.00, 401.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5446) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.92, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 37, 667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1963) [M::mem_pestat] mean and std.dev: (334.61, 550.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2611) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1067.208 CPU sec, 47.506 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670487, 57, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.94, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 33, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984) [M::mem_pestat] mean and std.dev: (100.94, 205.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1306) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1081.930 CPU sec, 65.460 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670789, 32, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.86, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 40, 518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1514) [M::mem_pestat] mean and std.dev: (104.08, 165.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2012) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1074.203 CPU sec, 47.906 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671478, 40, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.78, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 20, 67) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 177) [M::mem_pestat] mean and std.dev: (32.03, 32.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 232) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1088.188 CPU sec, 65.274 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670661, 35, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.83, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 35, 1550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4618) [M::mem_pestat] mean and std.dev: (554.19, 1114.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6152) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1062.117 CPU sec, 47.327 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671519, 48, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (494, 786, 3078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8246) [M::mem_pestat] mean and std.dev: (1164.67, 1091.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.06, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 48, 1586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4720) [M::mem_pestat] mean and std.dev: (783.13, 1409.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6420) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1093.817 CPU sec, 65.449 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671173, 50, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.90, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 32, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 427) [M::mem_pestat] mean and std.dev: (52.56, 83.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1058.652 CPU sec, 47.134 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671296, 28, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.07, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 28, 1171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3493) [M::mem_pestat] mean and std.dev: (356.40, 794.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4654) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1088.026 CPU sec, 65.103 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670851, 51, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.85, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 44, 1460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4346) [M::mem_pestat] mean and std.dev: (297.19, 719.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5789) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1061.428 CPU sec, 47.190 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671494, 35, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.91, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 38, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (31.33, 25.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1109.817 CPU sec, 69.602 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671242, 58, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.90, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 49, 1398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4142) [M::mem_pestat] mean and std.dev: (507.77, 925.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5514) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1109.997 CPU sec, 65.823 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670861, 44, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.74, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 46, 1133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3371) [M::mem_pestat] mean and std.dev: (506.57, 870.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4490) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.954 CPU sec, 47.358 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671219, 36, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.82, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 46, 960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2820) [M::mem_pestat] mean and std.dev: (347.00, 594.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3750) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1106.626 CPU sec, 65.783 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671281, 49, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.92, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 51, 2629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7845) [M::mem_pestat] mean and std.dev: (1117.66, 1714.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10453) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1055.899 CPU sec, 47.010 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670863, 39, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.90, 82.46) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 28, 173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 485) [M::mem_pestat] mean and std.dev: (54.31, 96.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1096.681 CPU sec, 65.810 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670609, 43, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.99, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 96, 1717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5101) [M::mem_pestat] mean and std.dev: (856.93, 1378.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6793) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1061.758 CPU sec, 47.303 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671184, 35, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.77, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 35, 785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2311) [M::mem_pestat] mean and std.dev: (244.00, 510.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3074) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (299, 795, 1368) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3506) [M::mem_pestat] mean and std.dev: (974.90, 706.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4575) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1098.416 CPU sec, 65.345 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671156, 44, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.86, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 44, 1647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4899) [M::mem_pestat] mean and std.dev: (701.07, 1294.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6525) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1069.786 CPU sec, 47.481 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670434, 56, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.94, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 45, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 240) [M::mem_pestat] mean and std.dev: (35.23, 26.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 315) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1094.923 CPU sec, 65.806 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671100, 52, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (435, 755, 1365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3225) [M::mem_pestat] mean and std.dev: (822.90, 591.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4155) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.82, 82.46) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 34, 184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (43.66, 79.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1068.468 CPU sec, 47.495 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671136, 44, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.79, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 50, 934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2748) [M::mem_pestat] mean and std.dev: (249.59, 490.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3655) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1100.535 CPU sec, 65.961 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670543, 46, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.92, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 56, 1012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3000) [M::mem_pestat] mean and std.dev: (290.88, 588.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3994) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1065.950 CPU sec, 47.487 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670417, 43, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.83, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 46, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 410) [M::mem_pestat] mean and std.dev: (49.66, 56.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 543) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1103.103 CPU sec, 65.655 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671126, 45, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 672) [M::mem_pestat] mean and std.dev: (398.23, 82.85) [M::mem_pestat] low and high boundaries for proper pairs: (12, 782) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 26, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 376) [M::mem_pestat] mean and std.dev: (45.77, 63.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 498) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1059.306 CPU sec, 47.147 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671260, 33, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.84, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 47, 2328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6954) [M::mem_pestat] mean and std.dev: (858.68, 1308.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9267) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1100.358 CPU sec, 65.154 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670970, 41, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.92, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 39, 1756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5234) [M::mem_pestat] mean and std.dev: (613.37, 1234.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6973) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1060.157 CPU sec, 47.152 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671194, 50, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (97, 426, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2146) [M::mem_pestat] mean and std.dev: (478.60, 405.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.92, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 32, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1065) [M::mem_pestat] mean and std.dev: (96.44, 218.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1414) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1075.783 CPU sec, 65.696 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670447, 49, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.92, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 34, 2831) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8471) [M::mem_pestat] mean and std.dev: (1316.06, 2265.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11291) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1071.758 CPU sec, 47.916 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 670688, 51, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 671, 964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2366) [M::mem_pestat] mean and std.dev: (630.33, 437.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3067) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.91, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 38, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 942) [M::mem_pestat] mean and std.dev: (101.17, 187.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1249) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1083.981 CPU sec, 64.480 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 670707, 29, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.99, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 28, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 197) [M::mem_pestat] mean and std.dev: (33.62, 31.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 259) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1066.269 CPU sec, 47.481 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670883, 49, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.77, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 34, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 234) [M::mem_pestat] mean and std.dev: (39.24, 35.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 306) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1083.543 CPU sec, 65.803 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 670906, 45, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (488, 676, 1417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3275) [M::mem_pestat] mean and std.dev: (934.46, 755.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4204) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.10, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 26, 63) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 153) [M::mem_pestat] mean and std.dev: (32.33, 27.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 198) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1065.560 CPU sec, 47.516 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670608, 43, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.08, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 50, 934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2760) [M::mem_pestat] mean and std.dev: (252.62, 553.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3673) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1084.297 CPU sec, 65.416 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 671375, 39, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (419, 468, 945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1997) [M::mem_pestat] mean and std.dev: (578.67, 256.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2523) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.73, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 37, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 258) [M::mem_pestat] mean and std.dev: (41.91, 48.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (976, 1295, 2679) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6085) [M::mem_pestat] mean and std.dev: (1354.18, 870.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7788) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1065.050 CPU sec, 47.301 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671028, 36, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.90, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 36, 471) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1381) [M::mem_pestat] mean and std.dev: (58.00, 102.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1836) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1091.348 CPU sec, 65.916 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671068, 42, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.73, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 25, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 198) [M::mem_pestat] mean and std.dev: (27.12, 25.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 261) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (296, 521, 1267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3209) [M::mem_pestat] mean and std.dev: (729.60, 481.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4180) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1066.889 CPU sec, 47.387 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670771, 30, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.02, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 38, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (46.17, 57.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1101.985 CPU sec, 66.602 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 670591, 41, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (339, 430, 620) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1182) [M::mem_pestat] mean and std.dev: (409.60, 208.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1463) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.85, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 75, 1674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4966) [M::mem_pestat] mean and std.dev: (589.00, 905.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6612) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1076.731 CPU sec, 47.739 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671621, 28, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.79, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 41, 1874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5588) [M::mem_pestat] mean and std.dev: (756.85, 1187.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7445) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (953, 1570, 2102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4400) [M::mem_pestat] mean and std.dev: (1368.00, 826.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5549) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1086.736 CPU sec, 65.093 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670943, 43, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.98, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 30, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 431) [M::mem_pestat] mean and std.dev: (44.94, 62.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1057.537 CPU sec, 47.199 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670670, 37, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.91, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 45, 110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 294) [M::mem_pestat] mean and std.dev: (40.72, 32.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 386) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1083.230 CPU sec, 64.472 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671242, 40, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 508, 948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2296) [M::mem_pestat] mean and std.dev: (418.75, 241.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2970) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.95, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 33, 1260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3750) [M::mem_pestat] mean and std.dev: (340.14, 748.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4995) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1071.759 CPU sec, 47.718 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671050, 47, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.94, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 58, 961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2823) [M::mem_pestat] mean and std.dev: (360.32, 740.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3754) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1083.507 CPU sec, 64.596 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 670837, 37, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 333, 1031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2581) [M::mem_pestat] mean and std.dev: (400.92, 317.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3356) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.06, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 35, 726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2146) [M::mem_pestat] mean and std.dev: (314.06, 582.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2856) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1058.886 CPU sec, 47.077 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671131, 39, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.92, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 56, 1694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5030) [M::mem_pestat] mean and std.dev: (762.47, 1306.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6698) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1105.177 CPU sec, 65.886 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670953, 36, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.91, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 69, 1012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2986) [M::mem_pestat] mean and std.dev: (257.43, 548.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3973) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1051.576 CPU sec, 46.858 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671312, 46, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.97, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 49, 1315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3903) [M::mem_pestat] mean and std.dev: (465.27, 954.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5197) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1096.325 CPU sec, 65.259 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671209, 55, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.93, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 32, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (42.31, 54.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1067.105 CPU sec, 47.611 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670785, 42, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.02, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 23, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (49.57, 83.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 669) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1096.106 CPU sec, 65.325 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671186, 49, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.86, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 47, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (78.22, 138.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1428) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1066.305 CPU sec, 47.532 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671124, 39, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.75, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 39, 61) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 147) [M::mem_pestat] mean and std.dev: (33.72, 25.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 190) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1099.628 CPU sec, 63.609 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 670787, 50, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.77, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 37, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1201) [M::mem_pestat] mean and std.dev: (137.57, 263.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1595) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1104.763 CPU sec, 65.964 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671285, 47, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.03, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 36, 59) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 139) [M::mem_pestat] mean and std.dev: (34.54, 27.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 179) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1062.819 CPU sec, 47.205 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670613, 43, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.80, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 46, 210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 580) [M::mem_pestat] mean and std.dev: (59.32, 65.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 765) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1099.558 CPU sec, 65.763 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671124, 52, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (362, 1045, 1194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2858) [M::mem_pestat] mean and std.dev: (906.44, 763.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3959) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 672) [M::mem_pestat] mean and std.dev: (398.10, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (12, 782) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 67, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (67.71, 91.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1065.364 CPU sec, 47.410 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670990, 47, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.97, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 44, 843) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2503) [M::mem_pestat] mean and std.dev: (299.15, 606.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3333) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1100.296 CPU sec, 66.425 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670815, 34, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.84, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 45, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 992) [M::mem_pestat] mean and std.dev: (79.07, 133.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1319) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1076.740 CPU sec, 47.874 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670837, 37, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.80, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 73, 1830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5418) [M::mem_pestat] mean and std.dev: (699.71, 966.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7212) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1102.020 CPU sec, 65.841 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670695, 40, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.94, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 30, 65) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 165) [M::mem_pestat] mean and std.dev: (31.44, 27.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 215) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1060.838 CPU sec, 47.158 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 670913, 44, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (378, 745, 1398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3438) [M::mem_pestat] mean and std.dev: (982.50, 854.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4458) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.94, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 40, 109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (36.31, 35.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 397) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1085.451 CPU sec, 66.025 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670689, 51, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.91, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 564) [M::mem_pestat] mean and std.dev: (57.76, 94.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 747) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1062.673 CPU sec, 47.478 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670835, 52, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.85, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 43, 818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2412) [M::mem_pestat] mean and std.dev: (227.62, 510.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3209) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1086.449 CPU sec, 66.045 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 671484, 38, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 551, 1347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3535) [M::mem_pestat] mean and std.dev: (1105.90, 1209.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5943) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.88, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 34, 311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 901) [M::mem_pestat] mean and std.dev: (97.84, 204.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1065.185 CPU sec, 47.388 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671484, 42, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.91, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 30, 2596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7766) [M::mem_pestat] mean and std.dev: (1080.71, 2004.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10351) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1087.936 CPU sec, 65.269 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 671144, 36, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.03, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 45, 1439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4275) [M::mem_pestat] mean and std.dev: (575.84, 1119.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5693) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1055.841 CPU sec, 46.926 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 671142, 57, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (491, 1103, 1982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4964) [M::mem_pestat] mean and std.dev: (1351.00, 1274.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.82, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 49, 718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2108) [M::mem_pestat] mean and std.dev: (156.93, 329.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2803) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1103.748 CPU sec, 65.744 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 670450, 50, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (427, 893, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2485) [M::mem_pestat] mean and std.dev: (726.00, 315.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3171) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 672) [M::mem_pestat] mean and std.dev: (398.07, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (12, 782) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 50, 2580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7696) [M::mem_pestat] mean and std.dev: (1172.25, 2089.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10254) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.126 CPU sec, 47.327 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671647, 39, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.99, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 28, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 661) [M::mem_pestat] mean and std.dev: (58.41, 112.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 877) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1082.564 CPU sec, 65.515 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670648, 45, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.87, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 40, 97) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259) [M::mem_pestat] mean and std.dev: (35.03, 30.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1056.047 CPU sec, 47.057 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671166, 52, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.88, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 37, 1320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3924) [M::mem_pestat] mean and std.dev: (400.93, 731.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5226) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1096.642 CPU sec, 65.749 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671663, 47, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.79, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 35, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 501) [M::mem_pestat] mean and std.dev: (55.66, 95.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 663) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1063.038 CPU sec, 47.340 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670928, 49, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.84, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 40, 910) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2704) [M::mem_pestat] mean and std.dev: (319.91, 703.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3601) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1094.423 CPU sec, 66.094 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671320, 52, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.77, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (8, 44, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1178) [M::mem_pestat] mean and std.dev: (63.00, 133.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1568) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1056.727 CPU sec, 47.012 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 670592, 36, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (402, 740, 1420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3456) [M::mem_pestat] mean and std.dev: (911.73, 877.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.01, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 61, 1898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5644) [M::mem_pestat] mean and std.dev: (887.89, 1353.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7517) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1108.076 CPU sec, 65.823 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670482, 47, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.88, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 58, 727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2141) [M::mem_pestat] mean and std.dev: (127.24, 253.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2848) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1068.061 CPU sec, 47.585 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 670873, 38, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.67, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 42, 797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2345) [M::mem_pestat] mean and std.dev: (314.17, 564.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3119) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1097.866 CPU sec, 65.909 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671324, 49, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.98, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 24, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 246) [M::mem_pestat] mean and std.dev: (22.74, 18.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 324) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1067.185 CPU sec, 47.417 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671754, 42, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.99, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 32, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 220) [M::mem_pestat] mean and std.dev: (32.97, 28.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 288) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1101.477 CPU sec, 66.919 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 670760, 47, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.88, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 36, 65) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 149) [M::mem_pestat] mean and std.dev: (35.44, 24.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 191) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1102.576 CPU sec, 66.291 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 670494, 41, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.97, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 54, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 335) [M::mem_pestat] mean and std.dev: (46.53, 37.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 439) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1075.530 CPU sec, 47.803 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670434, 38, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 672) [M::mem_pestat] mean and std.dev: (397.92, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (12, 782) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 43, 1531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4551) [M::mem_pestat] mean and std.dev: (724.28, 1362.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6175) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1092.561 CPU sec, 65.418 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 670473, 54, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.99, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 31, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 350) [M::mem_pestat] mean and std.dev: (47.33, 55.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 461) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1062.686 CPU sec, 47.353 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671554, 56, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.89, 82.45) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 40, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 793) [M::mem_pestat] mean and std.dev: (60.80, 82.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1052) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1068.147 CPU sec, 64.777 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 670782, 53, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (313, 1072, 1324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3346) [M::mem_pestat] mean and std.dev: (633.62, 449.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.99, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 51, 995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2955) [M::mem_pestat] mean and std.dev: (245.53, 538.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3935) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1062.764 CPU sec, 47.437 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671202, 56, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.15, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 42, 214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 608) [M::mem_pestat] mean and std.dev: (64.17, 103.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 805) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1079.601 CPU sec, 64.853 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 671443, 40, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.78, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 55, 268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 738) [M::mem_pestat] mean and std.dev: (54.61, 53.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 973) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1069.634 CPU sec, 47.730 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671253, 48, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 438, 1206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3056) [M::mem_pestat] mean and std.dev: (798.73, 715.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.94, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 33, 1481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4409) [M::mem_pestat] mean and std.dev: (575.19, 1236.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5873) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1096.626 CPU sec, 65.372 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671221, 46, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.95, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 69, 907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2673) [M::mem_pestat] mean and std.dev: (257.92, 477.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3556) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1064.522 CPU sec, 47.595 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 670837, 41, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (382, 741, 1165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2731) [M::mem_pestat] mean and std.dev: (781.64, 528.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.90, 82.44) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 33, 834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2458) [M::mem_pestat] mean and std.dev: (206.14, 510.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3270) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1095.103 CPU sec, 65.432 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671045, 50, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.96, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 33, 74) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 190) [M::mem_pestat] mean and std.dev: (31.82, 29.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 248) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1069.471 CPU sec, 47.606 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 670833, 43, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.00, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 35, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 467) [M::mem_pestat] mean and std.dev: (48.03, 71.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 619) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1095.156 CPU sec, 65.344 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 671282, 54, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.83, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 55, 658) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1930) [M::mem_pestat] mean and std.dev: (242.11, 525.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2566) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1058.666 CPU sec, 46.973 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 670636, 37, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (258, 359, 748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1728) [M::mem_pestat] mean and std.dev: (476.00, 324.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2218) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.94, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 59, 1969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5865) [M::mem_pestat] mean and std.dev: (698.14, 1147.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7813) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1096.689 CPU sec, 65.448 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 671161, 56, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 394, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (398.01, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 29, 50) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 126) [M::mem_pestat] mean and std.dev: (25.89, 17.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 164) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1058.730 CPU sec, 47.024 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671194, 42, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.91, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 28, 74) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 202) [M::mem_pestat] mean and std.dev: (31.12, 29.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 266) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1086.994 CPU sec, 64.542 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 670743, 52, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (682, 1210, 1777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3967) [M::mem_pestat] mean and std.dev: (1093.91, 643.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5062) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.87, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 38, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 163) [M::mem_pestat] mean and std.dev: (35.68, 26.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 210) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1066.454 CPU sec, 47.413 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670397, 52, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.91, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 40, 1072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3180) [M::mem_pestat] mean and std.dev: (377.15, 701.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4234) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1091.971 CPU sec, 65.171 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 671464, 36, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.89, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 58, 972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2872) [M::mem_pestat] mean and std.dev: (311.52, 650.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3822) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1063.211 CPU sec, 47.289 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670772, 54, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.95, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 45, 1183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3511) [M::mem_pestat] mean and std.dev: (465.10, 773.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4675) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1094.804 CPU sec, 65.307 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 670633, 39, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.95, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 55, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 615) [M::mem_pestat] mean and std.dev: (100.85, 131.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 810) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1044.750 CPU sec, 46.407 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 671179, 31, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (306, 460, 1286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3246) [M::mem_pestat] mean and std.dev: (737.77, 682.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4226) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.94, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 36, 247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 705) [M::mem_pestat] mean and std.dev: (44.21, 54.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 934) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1082.231 CPU sec, 64.523 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 671371, 44, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 397, 734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1738) [M::mem_pestat] mean and std.dev: (402.20, 247.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2240) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.89, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 44, 865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2551) [M::mem_pestat] mean and std.dev: (305.82, 555.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3394) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1051.550 CPU sec, 46.723 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 670819, 35, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.87, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 47, 1275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3799) [M::mem_pestat] mean and std.dev: (498.61, 956.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5061) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1096.676 CPU sec, 65.763 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 671209, 46, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.80, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 48, 1125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3329) [M::mem_pestat] mean and std.dev: (452.50, 836.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4431) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1053.269 CPU sec, 46.920 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::process] read 1600000 sequences (240000000 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671158, 39, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.88, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 42, 477) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1391) [M::mem_pestat] mean and std.dev: (104.25, 222.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1848) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1088.941 CPU sec, 66.181 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 671131, 65, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (342, 393, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 669) [M::mem_pestat] mean and std.dev: (397.75, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (15, 778) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 38, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 599) [M::mem_pestat] mean and std.dev: (56.54, 83.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 792) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1055.564 CPU sec, 46.997 real sec [M::process] read 9462 sequences (1419300 bp)... [M::process] 0 single-end sequences; 9462 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 3976, 0, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (343, 393, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (131, 661) [M::mem_pestat] mean and std.dev: (397.32, 81.10) [M::mem_pestat] low and high boundaries for proper pairs: (25, 767) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 9462 reads in 18.277 CPU sec, 0.816 real sec [main] Version: 0.7.17-r1188 [main] CMD: /home/csyk2proj/tools/bwa.kit/bwa mem -p -t24 -R@RG\tID:KPGP-00331\tPL:ILLUMINA\tSM:KPGP-00331 -C /home/csyk2proj/data/bwa.kit/hs38DH.fa - [main] Real time: 39652.971 sec; CPU: 484327.315 sec