[M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 653539, 30, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.47, 97.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 110, 2366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7042) [M::mem_pestat] mean and std.dev: (1023.83, 1706.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9380) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1061.934 CPU sec, 58.960 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654298, 41, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.27, 98.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (61, 845, 3114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9220) [M::mem_pestat] mean and std.dev: (1751.24, 2049.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12273) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1055.537 CPU sec, 46.845 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653859, 40, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.59, 97.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 773, 3801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11351) [M::mem_pestat] mean and std.dev: (2099.88, 2593.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15126) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1051.366 CPU sec, 56.853 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653855, 37, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.50, 97.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (43, 160, 3316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9862) [M::mem_pestat] mean and std.dev: (1920.65, 2816.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13186) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1043.361 CPU sec, 46.203 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 654450, 37, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.75, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 56, 2028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6034) [M::mem_pestat] mean and std.dev: (867.06, 1500.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8037) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1050.360 CPU sec, 57.082 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654221, 45, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.68, 98.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 51, 975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2899) [M::mem_pestat] mean and std.dev: (219.47, 461.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3861) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1043.664 CPU sec, 46.206 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654603, 45, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.63, 98.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 116, 4349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12973) [M::mem_pestat] mean and std.dev: (1706.80, 2338.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17285) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1057.762 CPU sec, 60.108 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 653993, 45, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (393, 1038, 2537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6825) [M::mem_pestat] mean and std.dev: (1577.10, 1406.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.68, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 40, 2365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7055) [M::mem_pestat] mean and std.dev: (730.83, 1327.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9400) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1048.955 CPU sec, 46.560 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654114, 36, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.74, 98.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 324, 4333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12917) [M::mem_pestat] mean and std.dev: (1901.50, 2345.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17209) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1052.138 CPU sec, 59.418 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654020, 44, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.32, 98.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 98, 1550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4592) [M::mem_pestat] mean and std.dev: (665.08, 1186.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6113) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1046.442 CPU sec, 46.417 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 653973, 38, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.49, 98.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 89, 1547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4599) [M::mem_pestat] mean and std.dev: (748.12, 1197.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6125) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1067.135 CPU sec, 61.978 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654156, 45, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.47, 98.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 77, 2652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7886) [M::mem_pestat] mean and std.dev: (1254.71, 1946.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10503) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1054.186 CPU sec, 59.321 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654615, 26, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.69, 98.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 188, 1733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5141) [M::mem_pestat] mean and std.dev: (819.36, 1265.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6845) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1047.333 CPU sec, 46.771 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654577, 41, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.64, 98.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 80, 2985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8909) [M::mem_pestat] mean and std.dev: (1679.90, 2666.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12347) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1059.296 CPU sec, 62.058 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654190, 32, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.70, 98.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 141, 3009) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8983) [M::mem_pestat] mean and std.dev: (1735.28, 2587.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12083) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1055.572 CPU sec, 47.058 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 653524, 37, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.56, 98.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 43, 2762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8266) [M::mem_pestat] mean and std.dev: (1231.61, 1993.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11018) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1061.747 CPU sec, 61.120 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654195, 52, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.49, 97.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 82, 3493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10447) [M::mem_pestat] mean and std.dev: (1677.00, 2748.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13924) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1056.997 CPU sec, 47.024 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 653884, 43, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (320, 435, 1229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3047) [M::mem_pestat] mean and std.dev: (779.44, 796.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3966) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.81, 98.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 67, 1545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4595) [M::mem_pestat] mean and std.dev: (823.32, 1433.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6556) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1066.833 CPU sec, 61.881 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654183, 36, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.56, 98.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 113, 3705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11033) [M::mem_pestat] mean and std.dev: (1602.83, 2347.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14697) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1046.264 CPU sec, 46.487 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 653937, 44, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.69, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 117, 1578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4680) [M::mem_pestat] mean and std.dev: (666.81, 1137.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6231) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1060.224 CPU sec, 61.039 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654275, 38, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.80, 98.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 91, 1983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5895) [M::mem_pestat] mean and std.dev: (959.54, 1484.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7851) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1049.112 CPU sec, 46.623 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 654516, 41, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (294, 400, 547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053) [M::mem_pestat] mean and std.dev: (391.08, 143.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1306) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.67, 98.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 50, 3099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9265) [M::mem_pestat] mean and std.dev: (1650.85, 2598.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12348) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1056.782 CPU sec, 60.616 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654074, 40, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.69, 98.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 67, 1737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5149) [M::mem_pestat] mean and std.dev: (870.41, 1471.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6855) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1057.592 CPU sec, 47.081 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 654344, 35, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.58, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 59, 988) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2900) [M::mem_pestat] mean and std.dev: (292.47, 594.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3856) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1050.241 CPU sec, 60.655 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654380, 42, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.67, 98.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 314, 1849) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5495) [M::mem_pestat] mean and std.dev: (1085.30, 1484.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7318) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1039.818 CPU sec, 46.146 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654509, 42, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.61, 98.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 124, 2012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5974) [M::mem_pestat] mean and std.dev: (1024.10, 1545.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7955) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1058.284 CPU sec, 59.244 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654349, 30, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.66, 98.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 482, 2489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7431) [M::mem_pestat] mean and std.dev: (1066.79, 1558.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9902) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1046.426 CPU sec, 46.412 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654606, 37, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.63, 98.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 333, 2302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6838) [M::mem_pestat] mean and std.dev: (1157.56, 1517.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9106) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1053.342 CPU sec, 60.747 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654075, 39, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.52, 97.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 90, 1925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5727) [M::mem_pestat] mean and std.dev: (853.61, 1405.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7628) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1047.932 CPU sec, 46.440 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654041, 40, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.37, 98.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 45, 713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2085) [M::mem_pestat] mean and std.dev: (72.06, 130.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2771) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1057.850 CPU sec, 60.365 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 654765, 42, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.78, 98.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 149, 3544) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10528) [M::mem_pestat] mean and std.dev: (2040.79, 2824.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14020) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1041.389 CPU sec, 46.080 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654071, 38, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.49, 97.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 204, 3957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11791) [M::mem_pestat] mean and std.dev: (1719.71, 2313.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15708) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1058.267 CPU sec, 61.002 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 653778, 40, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.72, 98.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 73, 2631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7849) [M::mem_pestat] mean and std.dev: (1178.34, 1786.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10458) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1049.163 CPU sec, 46.570 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653386, 37, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.41, 97.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 67, 1543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4565) [M::mem_pestat] mean and std.dev: (736.71, 1241.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6076) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1057.360 CPU sec, 61.334 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653657, 38, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.66, 98.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (42, 519, 2261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6699) [M::mem_pestat] mean and std.dev: (1229.46, 1601.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8918) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1038.464 CPU sec, 46.071 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 654404, 34, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.45, 97.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 45, 1906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5678) [M::mem_pestat] mean and std.dev: (640.06, 1150.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7564) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1052.372 CPU sec, 59.653 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654267, 46, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.79, 98.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 154, 1596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4746) [M::mem_pestat] mean and std.dev: (874.42, 1347.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6321) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1049.160 CPU sec, 46.783 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654399, 35, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.55, 97.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 35, 1519) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4529) [M::mem_pestat] mean and std.dev: (838.00, 1402.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6447) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1049.924 CPU sec, 60.145 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654263, 40, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.70, 98.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 464, 2163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6449) [M::mem_pestat] mean and std.dev: (993.03, 1489.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8592) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1056.312 CPU sec, 46.839 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 653373, 45, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.91, 98.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 545, 2964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8850) [M::mem_pestat] mean and std.dev: (1627.27, 2089.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11793) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1054.588 CPU sec, 60.191 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654493, 44, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.61, 98.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 71, 2319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6903) [M::mem_pestat] mean and std.dev: (997.10, 1565.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9195) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1038.488 CPU sec, 46.078 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654008, 44, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.55, 98.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 60, 2690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8038) [M::mem_pestat] mean and std.dev: (1271.59, 1886.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10712) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1053.634 CPU sec, 60.020 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654068, 45, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.42, 97.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 45, 3431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10261) [M::mem_pestat] mean and std.dev: (1622.98, 2730.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13676) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1031.423 CPU sec, 45.719 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654159, 36, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.67, 98.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 71, 1441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4265) [M::mem_pestat] mean and std.dev: (357.42, 628.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5677) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1039.141 CPU sec, 58.948 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654088, 40, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.76, 98.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 159, 2567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7651) [M::mem_pestat] mean and std.dev: (1291.08, 1900.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10193) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1035.543 CPU sec, 45.934 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653852, 44, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.78, 98.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 74, 1504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4466) [M::mem_pestat] mean and std.dev: (603.20, 1072.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5947) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1041.139 CPU sec, 58.113 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654226, 33, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.52, 98.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 73, 948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2790) [M::mem_pestat] mean and std.dev: (353.21, 553.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3711) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1031.179 CPU sec, 45.815 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654117, 47, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.69, 98.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 42, 1985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5921) [M::mem_pestat] mean and std.dev: (629.10, 1182.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7889) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1045.480 CPU sec, 59.378 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654090, 47, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.46, 97.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 62, 594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1748) [M::mem_pestat] mean and std.dev: (206.20, 373.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2325) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1049.944 CPU sec, 47.001 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654365, 38, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.59, 98.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 45, 983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2909) [M::mem_pestat] mean and std.dev: (243.53, 524.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3872) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1039.929 CPU sec, 59.350 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654657, 44, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.72, 98.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 136, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7762) [M::mem_pestat] mean and std.dev: (977.98, 1336.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10340) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1035.864 CPU sec, 46.102 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 653619, 36, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.76, 98.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 152, 1932) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5756) [M::mem_pestat] mean and std.dev: (901.77, 1378.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7668) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1057.351 CPU sec, 61.798 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653897, 33, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.35, 97.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 136, 1947) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5759) [M::mem_pestat] mean and std.dev: (829.42, 1281.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7665) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1039.350 CPU sec, 46.192 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654251, 34, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.53, 98.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (108, 1633, 3989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11751) [M::mem_pestat] mean and std.dev: (2281.09, 2424.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15632) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1047.934 CPU sec, 61.364 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654601, 42, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.81, 98.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 62, 1636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4864) [M::mem_pestat] mean and std.dev: (719.76, 1399.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6478) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1040.099 CPU sec, 46.229 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653997, 38, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.61, 97.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 102, 3514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10504) [M::mem_pestat] mean and std.dev: (1454.39, 2104.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13999) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1039.972 CPU sec, 58.823 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 654179, 45, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.65, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 148, 2960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8828) [M::mem_pestat] mean and std.dev: (1571.42, 2434.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11762) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1033.077 CPU sec, 45.933 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 653581, 45, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 525, 1309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3293) [M::mem_pestat] mean and std.dev: (677.56, 562.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4285) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.75, 98.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 198, 3007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8969) [M::mem_pestat] mean and std.dev: (1615.18, 2239.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11950) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1041.906 CPU sec, 59.519 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 653960, 40, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.65, 98.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 156, 2564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7612) [M::mem_pestat] mean and std.dev: (1392.50, 1762.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10136) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1029.775 CPU sec, 45.797 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 653970, 43, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.58, 98.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 57, 1208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3588) [M::mem_pestat] mean and std.dev: (481.68, 909.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4778) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1053.984 CPU sec, 60.205 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 653945, 33, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.72, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 81, 2186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6510) [M::mem_pestat] mean and std.dev: (952.66, 1276.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8672) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1041.624 CPU sec, 46.380 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654108, 33, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.76, 98.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 73, 1668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4960) [M::mem_pestat] mean and std.dev: (724.94, 1326.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6606) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1050.417 CPU sec, 60.630 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654628, 22, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.75, 98.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 41, 1494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4436) [M::mem_pestat] mean and std.dev: (510.15, 690.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5907) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1038.553 CPU sec, 46.216 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653921, 38, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.76, 98.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 156, 1815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5389) [M::mem_pestat] mean and std.dev: (759.82, 1215.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7176) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1051.815 CPU sec, 61.772 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 654612, 43, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.61, 98.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 86, 2120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6292) [M::mem_pestat] mean and std.dev: (686.97, 1172.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8378) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1028.506 CPU sec, 45.716 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654626, 36, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.63, 98.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 73, 1713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5099) [M::mem_pestat] mean and std.dev: (874.09, 1444.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6792) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1032.672 CPU sec, 58.329 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 653758, 39, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.68, 97.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 40, 1422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4244) [M::mem_pestat] mean and std.dev: (533.64, 885.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5655) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1029.939 CPU sec, 45.653 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 653961, 40, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.83, 98.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 55, 1749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5205) [M::mem_pestat] mean and std.dev: (642.27, 1115.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6933) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1026.312 CPU sec, 55.642 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654097, 35, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.70, 98.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 471, 1968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5876) [M::mem_pestat] mean and std.dev: (1090.52, 1576.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7830) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1021.815 CPU sec, 45.321 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654122, 27, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.56, 98.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 88, 1837) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5469) [M::mem_pestat] mean and std.dev: (1065.08, 1413.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7285) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1034.837 CPU sec, 57.976 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 654186, 30, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.56, 98.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 101, 1861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5559) [M::mem_pestat] mean and std.dev: (869.29, 1349.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7408) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1028.384 CPU sec, 45.537 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654375, 49, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.58, 98.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 93, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2156) [M::mem_pestat] mean and std.dev: (179.78, 341.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2866) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1028.601 CPU sec, 54.717 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654054, 48, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.80, 98.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 53, 1793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5337) [M::mem_pestat] mean and std.dev: (781.76, 1315.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7109) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1024.802 CPU sec, 45.533 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654195, 40, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.54, 98.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 78, 2632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7850) [M::mem_pestat] mean and std.dev: (1103.95, 1680.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10459) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1026.470 CPU sec, 55.188 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653951, 41, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.74, 98.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 58, 1780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5294) [M::mem_pestat] mean and std.dev: (818.33, 1247.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7051) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1023.242 CPU sec, 45.149 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 654433, 54, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (355, 670, 1903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4999) [M::mem_pestat] mean and std.dev: (1076.54, 1150.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6547) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.71, 98.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 231, 2515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7485) [M::mem_pestat] mean and std.dev: (1294.75, 1832.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9970) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1034.342 CPU sec, 56.883 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653653, 51, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.77, 98.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 69, 1192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3526) [M::mem_pestat] mean and std.dev: (476.79, 776.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4693) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1027.728 CPU sec, 45.459 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654311, 38, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.80, 98.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 96, 1532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4530) [M::mem_pestat] mean and std.dev: (938.19, 1455.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6761) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1028.250 CPU sec, 54.670 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654526, 32, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.58, 98.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 94, 2121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6333) [M::mem_pestat] mean and std.dev: (762.17, 1545.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8439) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1020.604 CPU sec, 45.331 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654019, 44, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.59, 98.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 108, 1480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4362) [M::mem_pestat] mean and std.dev: (628.15, 998.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5803) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1027.166 CPU sec, 57.077 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654040, 42, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.74, 98.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 48, 1383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4117) [M::mem_pestat] mean and std.dev: (511.68, 883.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5484) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1031.033 CPU sec, 45.858 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 654143, 52, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.65, 98.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 197, 2523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7531) [M::mem_pestat] mean and std.dev: (1211.37, 1597.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10035) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1032.490 CPU sec, 57.733 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 653989, 40, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.71, 98.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (52, 1506, 4355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12961) [M::mem_pestat] mean and std.dev: (1983.33, 2274.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17264) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1032.447 CPU sec, 45.880 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 654489, 34, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.42, 97.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 252, 1393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4121) [M::mem_pestat] mean and std.dev: (622.23, 910.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5485) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1022.856 CPU sec, 53.320 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 653980, 42, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.65, 98.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 51, 1024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3038) [M::mem_pestat] mean and std.dev: (362.49, 665.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4045) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1023.719 CPU sec, 45.500 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654385, 43, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.53, 98.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 87, 1040) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3074) [M::mem_pestat] mean and std.dev: (356.72, 690.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4091) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1025.308 CPU sec, 53.804 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654487, 44, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.79, 98.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 74, 1489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4411) [M::mem_pestat] mean and std.dev: (444.68, 817.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5872) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1022.429 CPU sec, 45.214 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 653660, 35, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.68, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 145, 2543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7585) [M::mem_pestat] mean and std.dev: (1394.97, 1828.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10106) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1027.622 CPU sec, 55.141 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654297, 31, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.75, 98.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 64, 2922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8718) [M::mem_pestat] mean and std.dev: (1736.00, 2751.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12740) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1023.530 CPU sec, 45.388 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654275, 34, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.74, 98.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 72, 3583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10697) [M::mem_pestat] mean and std.dev: (1792.82, 2544.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14254) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1039.029 CPU sec, 58.294 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 653616, 36, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.49, 97.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 150, 1536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4520) [M::mem_pestat] mean and std.dev: (727.97, 1195.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6012) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1032.007 CPU sec, 45.699 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 653889, 40, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.79, 98.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 64, 3581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10691) [M::mem_pestat] mean and std.dev: (1375.70, 1870.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14246) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1030.790 CPU sec, 57.295 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654103, 49, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.81, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 133, 4041) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12071) [M::mem_pestat] mean and std.dev: (1768.41, 2645.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16086) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1031.328 CPU sec, 45.834 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 653178, 40, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1106, 2623, 3647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8729) [M::mem_pestat] mean and std.dev: (2611.80, 1786.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11270) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.75, 97.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 156, 2348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6974) [M::mem_pestat] mean and std.dev: (864.84, 1250.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9287) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1041.070 CPU sec, 58.691 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654119, 36, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.82, 98.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 36, 611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1795) [M::mem_pestat] mean and std.dev: (91.43, 165.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2387) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1032.299 CPU sec, 45.834 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653722, 39, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.43, 97.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 66, 1257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3725) [M::mem_pestat] mean and std.dev: (467.94, 836.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4959) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1044.162 CPU sec, 58.639 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654152, 40, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.64, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 40, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 354) [M::mem_pestat] mean and std.dev: (46.76, 42.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1032.736 CPU sec, 45.990 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 654282, 31, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.90, 98.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 55, 2940) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8790) [M::mem_pestat] mean and std.dev: (1276.97, 1705.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11715) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1039.239 CPU sec, 58.994 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 653721, 35, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.82, 98.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 56, 2071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6179) [M::mem_pestat] mean and std.dev: (657.72, 1119.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8233) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1032.000 CPU sec, 45.945 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653823, 46, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.37, 97.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 128, 2387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7109) [M::mem_pestat] mean and std.dev: (1082.73, 1587.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9470) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1036.515 CPU sec, 59.591 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 653477, 40, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.68, 98.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 77, 1463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4327) [M::mem_pestat] mean and std.dev: (449.32, 825.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5759) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1028.504 CPU sec, 45.779 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654184, 34, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.31, 97.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 40, 760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2256) [M::mem_pestat] mean and std.dev: (220.97, 446.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3004) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1031.299 CPU sec, 57.993 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654501, 35, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.69, 98.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 122, 2683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8003) [M::mem_pestat] mean and std.dev: (1084.56, 1575.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10663) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1029.051 CPU sec, 45.606 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653840, 60, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.49, 97.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 95, 1084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3194) [M::mem_pestat] mean and std.dev: (364.60, 623.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4249) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1039.096 CPU sec, 58.503 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654212, 42, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.28, 97.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (45, 689, 3270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9720) [M::mem_pestat] mean and std.dev: (1913.43, 2417.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12945) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1024.010 CPU sec, 45.391 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654336, 48, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.61, 98.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 40, 637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1877) [M::mem_pestat] mean and std.dev: (252.95, 466.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2497) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1040.562 CPU sec, 58.607 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653794, 40, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.52, 97.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 46, 2637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7879) [M::mem_pestat] mean and std.dev: (890.47, 1629.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10500) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1031.953 CPU sec, 45.958 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654809, 46, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.78, 98.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 84, 1543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4569) [M::mem_pestat] mean and std.dev: (1030.96, 1622.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7521) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1036.786 CPU sec, 59.414 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653659, 45, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.40, 97.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 73, 1795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5337) [M::mem_pestat] mean and std.dev: (940.66, 1434.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7108) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1024.908 CPU sec, 45.460 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654282, 38, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.50, 98.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 80, 924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2722) [M::mem_pestat] mean and std.dev: (300.72, 483.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3621) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1035.981 CPU sec, 59.356 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 653659, 45, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.51, 98.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 121, 2591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7693) [M::mem_pestat] mean and std.dev: (1146.90, 1750.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10244) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1026.751 CPU sec, 45.553 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654258, 40, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.69, 98.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 100, 1198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3540) [M::mem_pestat] mean and std.dev: (488.26, 908.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4711) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1027.843 CPU sec, 57.060 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 653575, 46, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.85, 98.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 60, 1156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3428) [M::mem_pestat] mean and std.dev: (388.35, 767.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4564) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1024.191 CPU sec, 45.352 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 653351, 39, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.58, 98.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 392, 4098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12232) [M::mem_pestat] mean and std.dev: (1909.15, 2405.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16299) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1024.613 CPU sec, 56.855 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654045, 40, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.64, 98.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 92, 1516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4508) [M::mem_pestat] mean and std.dev: (667.19, 1034.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6004) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1023.392 CPU sec, 45.290 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654014, 41, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.76, 98.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 87, 3286) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9828) [M::mem_pestat] mean and std.dev: (1671.05, 2385.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13099) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1022.435 CPU sec, 56.810 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654816, 40, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.71, 98.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 70, 1698) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5038) [M::mem_pestat] mean and std.dev: (728.03, 1300.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6708) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1016.886 CPU sec, 45.192 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654673, 57, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.59, 98.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 111, 2100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6246) [M::mem_pestat] mean and std.dev: (983.77, 1431.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8319) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1023.558 CPU sec, 55.868 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654444, 44, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.61, 98.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 497, 3026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9026) [M::mem_pestat] mean and std.dev: (1316.98, 1893.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12026) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1027.151 CPU sec, 45.514 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654012, 39, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.73, 98.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 59, 1034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3064) [M::mem_pestat] mean and std.dev: (409.94, 688.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4079) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1040.563 CPU sec, 59.506 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 653848, 46, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.67, 98.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 50, 189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 545) [M::mem_pestat] mean and std.dev: (54.64, 72.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 723) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1035.686 CPU sec, 46.286 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 654383, 38, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.66, 98.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 59, 958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2844) [M::mem_pestat] mean and std.dev: (309.18, 637.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3787) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1039.665 CPU sec, 58.792 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 653775, 37, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.53, 97.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 71, 2120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6308) [M::mem_pestat] mean and std.dev: (1166.46, 1592.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8402) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1033.149 CPU sec, 45.845 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653557, 44, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.64, 97.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 55, 1448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4304) [M::mem_pestat] mean and std.dev: (562.52, 1081.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5732) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1041.539 CPU sec, 60.941 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653575, 44, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.76, 98.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 145, 1922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5730) [M::mem_pestat] mean and std.dev: (1018.24, 1442.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7634) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1037.133 CPU sec, 46.131 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654808, 54, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.73, 98.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 76, 2577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7679) [M::mem_pestat] mean and std.dev: (1147.96, 1779.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10230) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1036.340 CPU sec, 58.931 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654685, 44, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.43, 98.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 80, 652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1908) [M::mem_pestat] mean and std.dev: (264.63, 470.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2536) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1026.196 CPU sec, 45.693 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 653886, 49, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.54, 97.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 40, 4160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12442) [M::mem_pestat] mean and std.dev: (1342.10, 2280.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16583) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1041.695 CPU sec, 58.578 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 653900, 45, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.41, 97.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 123, 3195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9519) [M::mem_pestat] mean and std.dev: (1588.95, 2420.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12681) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1031.348 CPU sec, 45.868 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654763, 34, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.79, 98.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 45, 1187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3533) [M::mem_pestat] mean and std.dev: (344.93, 785.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4706) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1032.092 CPU sec, 59.802 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654042, 36, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.52, 98.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 98, 2707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8061) [M::mem_pestat] mean and std.dev: (1050.81, 1676.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10738) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1026.408 CPU sec, 45.614 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653911, 47, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.58, 98.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 32, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1389) [M::mem_pestat] mean and std.dev: (87.81, 167.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1847) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1049.816 CPU sec, 59.818 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 653966, 42, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.80, 98.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 97, 1279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3783) [M::mem_pestat] mean and std.dev: (496.73, 844.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5035) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1035.074 CPU sec, 45.897 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654259, 34, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.66, 98.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 47, 2680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7990) [M::mem_pestat] mean and std.dev: (1350.58, 2120.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10645) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1042.065 CPU sec, 59.850 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653661, 43, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.70, 98.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 79, 1612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4780) [M::mem_pestat] mean and std.dev: (888.76, 1474.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6786) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1025.489 CPU sec, 45.561 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654744, 35, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.75, 97.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 74, 1160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3422) [M::mem_pestat] mean and std.dev: (510.28, 865.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4553) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1027.471 CPU sec, 58.730 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654972, 41, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.60, 98.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 210, 2683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7987) [M::mem_pestat] mean and std.dev: (1319.10, 1888.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10639) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1015.779 CPU sec, 44.964 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 654569, 44, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.59, 98.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 129, 1518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4498) [M::mem_pestat] mean and std.dev: (725.78, 1265.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5988) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1027.331 CPU sec, 55.675 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654090, 41, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.60, 98.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 226, 3523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10505) [M::mem_pestat] mean and std.dev: (1714.88, 2040.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13996) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1026.018 CPU sec, 45.573 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 653456, 39, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.77, 97.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 36, 1331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3963) [M::mem_pestat] mean and std.dev: (298.48, 588.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5279) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1035.386 CPU sec, 57.137 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654340, 36, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.55, 98.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 67, 2151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6395) [M::mem_pestat] mean and std.dev: (895.03, 1382.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8517) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1028.036 CPU sec, 45.644 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 654704, 38, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.95, 98.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 95, 1396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4154) [M::mem_pestat] mean and std.dev: (400.25, 728.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5533) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1025.211 CPU sec, 58.017 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653854, 50, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.73, 98.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 119, 3574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10682) [M::mem_pestat] mean and std.dev: (1756.90, 2765.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14236) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1030.934 CPU sec, 46.055 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 653989, 35, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.74, 97.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 111, 2693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8047) [M::mem_pestat] mean and std.dev: (1286.51, 1750.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10724) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1036.561 CPU sec, 59.247 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654321, 35, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.51, 98.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 122, 4346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12992) [M::mem_pestat] mean and std.dev: (2106.69, 2964.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17315) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1027.603 CPU sec, 45.701 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 653927, 30, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.74, 98.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 119, 1089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3219) [M::mem_pestat] mean and std.dev: (250.00, 412.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4284) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1035.691 CPU sec, 57.201 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 654267, 42, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.49, 97.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 145, 1686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5016) [M::mem_pestat] mean and std.dev: (985.29, 1552.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7196) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1024.803 CPU sec, 45.417 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654098, 53, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.73, 98.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 112, 3436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10260) [M::mem_pestat] mean and std.dev: (1712.42, 2538.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13672) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1033.300 CPU sec, 58.427 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654085, 38, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.73, 98.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 117, 815) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2389) [M::mem_pestat] mean and std.dev: (240.77, 396.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3176) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1022.021 CPU sec, 45.230 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654086, 31, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.64, 98.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 50, 860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2538) [M::mem_pestat] mean and std.dev: (166.00, 368.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3377) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1023.328 CPU sec, 57.903 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654515, 39, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.66, 98.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 54, 1968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5876) [M::mem_pestat] mean and std.dev: (988.87, 1512.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7830) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1022.836 CPU sec, 45.473 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653716, 36, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.68, 98.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 52, 1192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3536) [M::mem_pestat] mean and std.dev: (506.28, 941.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4708) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1024.388 CPU sec, 57.560 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 654073, 35, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.84, 98.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 146, 2607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7757) [M::mem_pestat] mean and std.dev: (1290.91, 2105.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10332) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1023.534 CPU sec, 45.418 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 654282, 40, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.87, 98.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 208, 1894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5588) [M::mem_pestat] mean and std.dev: (951.72, 1378.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7435) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1024.434 CPU sec, 57.306 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654604, 49, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.60, 98.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 140, 3236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9670) [M::mem_pestat] mean and std.dev: (1733.57, 2522.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12887) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1021.980 CPU sec, 45.448 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 654130, 40, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (343, 604, 1897) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5005) [M::mem_pestat] mean and std.dev: (799.22, 646.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6559) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.54, 98.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 162, 3192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9522) [M::mem_pestat] mean and std.dev: (1856.20, 2761.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12902) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1043.465 CPU sec, 59.520 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 654335, 42, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.76, 98.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 76, 2703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8083) [M::mem_pestat] mean and std.dev: (1444.67, 2194.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10773) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1032.939 CPU sec, 46.027 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654647, 32, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.46, 97.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 409, 4385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13093) [M::mem_pestat] mean and std.dev: (2185.56, 2768.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17447) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1034.455 CPU sec, 58.731 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 653424, 29, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.56, 98.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 58, 1329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3941) [M::mem_pestat] mean and std.dev: (487.26, 894.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5247) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1038.560 CPU sec, 46.266 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653671, 36, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.56, 97.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 64, 1513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4487) [M::mem_pestat] mean and std.dev: (544.67, 982.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5974) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1034.299 CPU sec, 58.722 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654270, 50, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.39, 97.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 94, 2408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7190) [M::mem_pestat] mean and std.dev: (1170.59, 1785.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9581) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1025.327 CPU sec, 45.293 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654063, 49, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.61, 98.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 109, 2382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7118) [M::mem_pestat] mean and std.dev: (997.41, 1622.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9486) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1025.670 CPU sec, 57.207 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 654061, 46, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.50, 97.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 57, 1582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4714) [M::mem_pestat] mean and std.dev: (770.40, 1346.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6280) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1022.260 CPU sec, 45.380 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 654500, 43, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.74, 98.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 52, 1424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4214) [M::mem_pestat] mean and std.dev: (623.77, 987.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5609) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1028.050 CPU sec, 56.557 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 653799, 32, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.44, 98.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 53, 2250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6688) [M::mem_pestat] mean and std.dev: (1044.30, 1624.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8907) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1026.633 CPU sec, 45.485 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654269, 40, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.68, 98.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 140, 1948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5796) [M::mem_pestat] mean and std.dev: (1007.74, 1556.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7720) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1022.355 CPU sec, 57.219 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654022, 31, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.77, 98.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 59, 2611) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7793) [M::mem_pestat] mean and std.dev: (779.50, 1378.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10384) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1025.919 CPU sec, 45.503 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654439, 37, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.47, 97.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 48, 2362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7058) [M::mem_pestat] mean and std.dev: (1172.28, 1824.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9406) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1033.326 CPU sec, 58.319 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653413, 47, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.57, 97.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 89, 657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1911) [M::mem_pestat] mean and std.dev: (309.72, 502.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2538) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1046.136 CPU sec, 46.467 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654017, 30, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.73, 98.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 112, 2461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7323) [M::mem_pestat] mean and std.dev: (1156.72, 1617.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9754) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1040.547 CPU sec, 61.179 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654903, 37, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.68, 98.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 80, 2262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6736) [M::mem_pestat] mean and std.dev: (911.94, 1405.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8973) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1034.759 CPU sec, 46.040 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654129, 40, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.46, 97.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 449, 4340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12954) [M::mem_pestat] mean and std.dev: (2122.55, 2955.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17261) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1032.576 CPU sec, 60.620 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654202, 52, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.67, 98.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 48, 2420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7216) [M::mem_pestat] mean and std.dev: (1177.04, 1690.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9614) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1028.074 CPU sec, 45.737 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653972, 44, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.81, 98.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 44, 773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2273) [M::mem_pestat] mean and std.dev: (246.18, 482.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3023) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1023.933 CPU sec, 58.362 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 653587, 51, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.80, 98.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 34, 1909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5699) [M::mem_pestat] mean and std.dev: (714.11, 1294.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7594) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1026.304 CPU sec, 45.741 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654123, 46, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.70, 98.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 122, 1835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5477) [M::mem_pestat] mean and std.dev: (991.74, 1499.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7298) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1030.558 CPU sec, 58.156 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654040, 37, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.54, 98.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 37, 1461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4357) [M::mem_pestat] mean and std.dev: (530.18, 904.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5805) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1021.984 CPU sec, 45.355 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654561, 44, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.72, 98.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 122, 3959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11811) [M::mem_pestat] mean and std.dev: (1644.11, 2541.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15737) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1018.920 CPU sec, 56.981 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 653300, 25, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.62, 98.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 30, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 882) [M::mem_pestat] mean and std.dev: (45.30, 79.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1173) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1024.051 CPU sec, 45.508 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654067, 42, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.73, 98.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 216, 2861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8521) [M::mem_pestat] mean and std.dev: (1519.02, 2106.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11351) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1025.463 CPU sec, 56.369 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653852, 43, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.44, 98.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 88, 2711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8065) [M::mem_pestat] mean and std.dev: (1248.85, 1865.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10742) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1029.049 CPU sec, 45.727 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654111, 45, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.32, 97.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 45, 1857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5537) [M::mem_pestat] mean and std.dev: (832.91, 1387.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7377) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1021.797 CPU sec, 56.339 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 653704, 42, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.80, 98.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 49, 1457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4331) [M::mem_pestat] mean and std.dev: (458.54, 998.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5768) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1029.602 CPU sec, 45.759 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 654443, 38, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.55, 98.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 106, 1640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4870) [M::mem_pestat] mean and std.dev: (845.74, 1208.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6485) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1026.072 CPU sec, 55.940 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 654603, 38, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.78, 98.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 109, 2642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7884) [M::mem_pestat] mean and std.dev: (1289.05, 1919.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10505) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1022.542 CPU sec, 45.485 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 654352, 45, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.73, 98.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 58, 636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1850) [M::mem_pestat] mean and std.dev: (157.05, 361.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2457) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1023.175 CPU sec, 56.623 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654115, 45, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.79, 98.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 112, 4197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12533) [M::mem_pestat] mean and std.dev: (1767.22, 2570.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16701) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1030.869 CPU sec, 45.910 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653386, 49, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.60, 98.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 69, 1739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5185) [M::mem_pestat] mean and std.dev: (706.15, 1303.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6908) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1030.963 CPU sec, 58.493 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653758, 45, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.55, 98.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 63, 1594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4734) [M::mem_pestat] mean and std.dev: (647.71, 1072.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6304) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1033.754 CPU sec, 45.966 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654072, 41, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.76, 98.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 108, 4353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13027) [M::mem_pestat] mean and std.dev: (2031.66, 2803.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17364) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1030.416 CPU sec, 57.316 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653959, 58, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.43, 97.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 45, 761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2253) [M::mem_pestat] mean and std.dev: (243.43, 446.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2999) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1023.048 CPU sec, 45.386 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654156, 28, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.33, 97.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 123, 2693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8027) [M::mem_pestat] mean and std.dev: (1091.30, 1567.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10694) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1022.718 CPU sec, 56.640 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 653633, 51, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 315, 809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2069) [M::mem_pestat] mean and std.dev: (410.60, 377.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2699) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.72, 98.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 60, 1583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4697) [M::mem_pestat] mean and std.dev: (785.23, 1359.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6254) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1024.646 CPU sec, 45.391 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653559, 38, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.61, 98.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 369, 2637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7841) [M::mem_pestat] mean and std.dev: (1266.03, 1833.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10443) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1029.715 CPU sec, 57.635 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654383, 58, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.88, 98.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (63, 638, 3208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9498) [M::mem_pestat] mean and std.dev: (1792.84, 2272.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12643) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1024.013 CPU sec, 45.320 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654102, 45, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.58, 98.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 96, 1240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3668) [M::mem_pestat] mean and std.dev: (477.07, 894.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4882) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1023.545 CPU sec, 55.972 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 653813, 38, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.61, 98.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 103, 1613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4797) [M::mem_pestat] mean and std.dev: (800.86, 1380.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6389) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1030.067 CPU sec, 45.756 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653747, 44, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.49, 98.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 38, 1236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3690) [M::mem_pestat] mean and std.dev: (374.52, 725.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4917) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1024.997 CPU sec, 56.656 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 653980, 43, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.71, 98.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 115, 1876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5580) [M::mem_pestat] mean and std.dev: (614.47, 1060.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7432) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1031.108 CPU sec, 45.665 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654343, 36, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.80, 98.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 104, 2849) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8459) [M::mem_pestat] mean and std.dev: (1320.37, 1913.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11264) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1022.616 CPU sec, 55.572 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654427, 35, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.50, 97.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 103, 2518) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7514) [M::mem_pestat] mean and std.dev: (1112.34, 1816.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10012) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1019.036 CPU sec, 45.142 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654455, 45, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.54, 98.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 143, 2421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7205) [M::mem_pestat] mean and std.dev: (1020.07, 1574.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9597) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1025.967 CPU sec, 56.902 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654577, 52, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.46, 98.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 103, 1587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4691) [M::mem_pestat] mean and std.dev: (693.42, 1224.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6243) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1021.368 CPU sec, 45.268 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 653833, 48, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.70, 98.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 62, 1968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5856) [M::mem_pestat] mean and std.dev: (544.75, 1258.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7800) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1029.361 CPU sec, 56.993 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654057, 32, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.82, 98.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 116, 2424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7202) [M::mem_pestat] mean and std.dev: (1236.53, 1933.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9591) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1024.662 CPU sec, 45.357 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 653884, 45, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.59, 98.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 392, 2327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6931) [M::mem_pestat] mean and std.dev: (1244.69, 1555.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9233) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1035.080 CPU sec, 58.544 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654536, 38, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.46, 97.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 55, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1335) [M::mem_pestat] mean and std.dev: (103.50, 198.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1772) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1023.408 CPU sec, 45.462 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654039, 38, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.77, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 76, 1270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3786) [M::mem_pestat] mean and std.dev: (652.53, 1101.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5057) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1026.215 CPU sec, 56.724 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654210, 49, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.61, 98.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 72, 1930) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5724) [M::mem_pestat] mean and std.dev: (914.35, 1415.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7621) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1023.900 CPU sec, 45.412 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 654855, 30, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (346, 623, 1680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4348) [M::mem_pestat] mean and std.dev: (637.00, 481.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.54, 97.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (53, 137, 1489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4361) [M::mem_pestat] mean and std.dev: (606.89, 961.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5797) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1021.459 CPU sec, 58.015 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654196, 43, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.69, 98.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 410, 3414) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10182) [M::mem_pestat] mean and std.dev: (1828.60, 2571.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13566) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1032.701 CPU sec, 45.913 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 653949, 54, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.55, 98.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 56, 1272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3760) [M::mem_pestat] mean and std.dev: (458.62, 827.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5004) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1029.306 CPU sec, 57.343 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654277, 50, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.53, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 61, 2652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7912) [M::mem_pestat] mean and std.dev: (1073.70, 1686.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10542) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1026.943 CPU sec, 45.565 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 653478, 47, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.61, 98.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 50, 916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2710) [M::mem_pestat] mean and std.dev: (265.15, 575.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3607) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1023.657 CPU sec, 57.670 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654776, 46, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.62, 98.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 59, 2089) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6235) [M::mem_pestat] mean and std.dev: (952.14, 1488.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8308) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1027.732 CPU sec, 45.543 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653768, 56, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.64, 98.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 109, 4364) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13038) [M::mem_pestat] mean and std.dev: (2033.36, 2826.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17375) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1031.066 CPU sec, 58.703 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 653972, 36, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.74, 98.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 94, 2903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8647) [M::mem_pestat] mean and std.dev: (1576.94, 2277.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11519) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1031.035 CPU sec, 45.691 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654238, 47, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.83, 98.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 59, 2663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7951) [M::mem_pestat] mean and std.dev: (1029.87, 1678.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10595) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1035.284 CPU sec, 58.881 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654312, 43, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.68, 98.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 68, 2190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6520) [M::mem_pestat] mean and std.dev: (810.27, 1381.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8685) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1033.322 CPU sec, 45.820 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 653496, 45, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.56, 98.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 91, 2644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7894) [M::mem_pestat] mean and std.dev: (1356.55, 1879.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10519) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1038.638 CPU sec, 59.666 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 654186, 47, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.85, 98.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 256, 3261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9739) [M::mem_pestat] mean and std.dev: (1626.74, 2225.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12978) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1028.069 CPU sec, 45.439 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 654057, 40, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.39, 98.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 86, 1950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5796) [M::mem_pestat] mean and std.dev: (872.69, 1377.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7719) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1039.189 CPU sec, 59.680 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654458, 34, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.60, 98.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 116, 2073) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6167) [M::mem_pestat] mean and std.dev: (1138.48, 1658.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8214) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1039.943 CPU sec, 46.238 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654359, 44, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.66, 98.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 91, 2253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6727) [M::mem_pestat] mean and std.dev: (825.43, 1415.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8964) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1044.947 CPU sec, 60.035 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653695, 39, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.80, 98.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 83, 2034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6040) [M::mem_pestat] mean and std.dev: (1052.81, 1528.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8043) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1039.247 CPU sec, 46.222 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654607, 43, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.88, 98.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 85, 2634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7848) [M::mem_pestat] mean and std.dev: (986.27, 1643.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10455) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1038.328 CPU sec, 59.937 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654614, 37, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.60, 98.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 60, 1933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5735) [M::mem_pestat] mean and std.dev: (942.60, 1635.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7636) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1036.609 CPU sec, 45.935 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 654724, 42, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.82, 98.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 47, 1884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5616) [M::mem_pestat] mean and std.dev: (818.12, 1437.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7482) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1039.266 CPU sec, 59.727 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 653927, 41, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.56, 98.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 773, 3102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9258) [M::mem_pestat] mean and std.dev: (1807.83, 2378.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12336) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1039.250 CPU sec, 46.278 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 654482, 35, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (388, 566, 2380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6364) [M::mem_pestat] mean and std.dev: (1327.50, 1329.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8356) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.76, 98.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 54, 4389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13109) [M::mem_pestat] mean and std.dev: (2274.37, 3378.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17469) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1034.863 CPU sec, 59.761 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654064, 50, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.56, 98.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 220, 2515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7501) [M::mem_pestat] mean and std.dev: (1156.71, 1564.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9994) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1042.258 CPU sec, 46.404 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 654336, 40, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.58, 98.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 105, 3813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11389) [M::mem_pestat] mean and std.dev: (1505.75, 2093.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15177) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1042.161 CPU sec, 60.392 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653976, 53, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.63, 98.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 155, 2774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8258) [M::mem_pestat] mean and std.dev: (1248.79, 1888.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11000) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1044.389 CPU sec, 46.420 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654351, 57, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.59, 98.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 54, 2095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6245) [M::mem_pestat] mean and std.dev: (773.84, 1321.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8320) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1035.988 CPU sec, 60.345 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 654264, 39, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.72, 98.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 224, 2673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7973) [M::mem_pestat] mean and std.dev: (1367.00, 1811.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10623) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1038.827 CPU sec, 46.170 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654511, 36, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.71, 98.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 119, 2985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8921) [M::mem_pestat] mean and std.dev: (1649.53, 2367.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11889) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1037.097 CPU sec, 58.801 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654343, 38, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.59, 98.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 75, 1026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3026) [M::mem_pestat] mean and std.dev: (404.18, 737.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4026) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1042.984 CPU sec, 46.535 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654085, 48, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.84, 98.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 51, 1786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5326) [M::mem_pestat] mean and std.dev: (627.07, 1105.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7096) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1036.299 CPU sec, 59.701 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 653498, 46, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.94, 98.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 172, 2247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6687) [M::mem_pestat] mean and std.dev: (971.51, 1459.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8907) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1040.238 CPU sec, 46.183 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654186, 29, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.31, 98.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 46, 3051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9119) [M::mem_pestat] mean and std.dev: (1632.72, 2560.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12153) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1034.501 CPU sec, 59.448 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654535, 39, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.47, 98.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 57, 1676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4980) [M::mem_pestat] mean and std.dev: (880.84, 1388.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6632) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1039.073 CPU sec, 46.391 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654230, 42, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.45, 97.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 107, 3028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9020) [M::mem_pestat] mean and std.dev: (1571.05, 2307.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12016) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1042.020 CPU sec, 60.885 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 654625, 46, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.43, 98.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 83, 1968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5864) [M::mem_pestat] mean and std.dev: (937.59, 1489.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7812) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1037.216 CPU sec, 45.823 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 654244, 45, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (256, 484, 717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1639) [M::mem_pestat] mean and std.dev: (506.10, 325.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2100) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.64, 98.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 65, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1647) [M::mem_pestat] mean and std.dev: (148.33, 275.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2189) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1037.640 CPU sec, 61.032 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654072, 43, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.64, 98.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 51, 1574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4686) [M::mem_pestat] mean and std.dev: (666.85, 1198.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6242) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1042.442 CPU sec, 46.357 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653778, 50, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.56, 98.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 88, 816) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2386) [M::mem_pestat] mean and std.dev: (241.49, 431.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3171) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1042.173 CPU sec, 60.929 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654386, 41, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.14, 97.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 127, 1506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4460) [M::mem_pestat] mean and std.dev: (567.69, 1075.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5937) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1041.736 CPU sec, 46.207 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 653625, 40, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.81, 98.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 58, 673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1983) [M::mem_pestat] mean and std.dev: (172.06, 301.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2638) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1036.525 CPU sec, 59.236 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654310, 33, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.54, 98.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 79, 3448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10290) [M::mem_pestat] mean and std.dev: (1703.06, 2605.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13711) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1042.099 CPU sec, 46.422 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 654057, 46, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (498, 583, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2343) [M::mem_pestat] mean and std.dev: (703.00, 321.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2958) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.75, 98.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 77, 1413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4195) [M::mem_pestat] mean and std.dev: (572.79, 963.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5586) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1030.660 CPU sec, 59.466 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654686, 43, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.67, 98.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 60, 923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2723) [M::mem_pestat] mean and std.dev: (381.13, 654.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3623) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1038.163 CPU sec, 46.238 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654425, 40, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.59, 98.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 99, 3287) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9797) [M::mem_pestat] mean and std.dev: (1565.78, 2458.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13052) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1041.507 CPU sec, 59.628 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654185, 36, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.97, 98.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 70, 2046) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6072) [M::mem_pestat] mean and std.dev: (930.56, 1471.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8085) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1039.344 CPU sec, 46.065 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 653950, 39, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.52, 97.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 126, 2592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7720) [M::mem_pestat] mean and std.dev: (1033.84, 1568.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10284) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1044.494 CPU sec, 60.942 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654781, 35, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.74, 98.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 66, 2420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7192) [M::mem_pestat] mean and std.dev: (1034.03, 1688.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9578) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1028.882 CPU sec, 45.632 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654112, 41, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.76, 98.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 183, 2565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7635) [M::mem_pestat] mean and std.dev: (1034.92, 1439.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10170) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1036.847 CPU sec, 59.298 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654011, 49, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.66, 98.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 117, 2529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7539) [M::mem_pestat] mean and std.dev: (1293.96, 1918.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10044) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1036.685 CPU sec, 45.913 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654261, 36, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.48, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 178, 2874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8560) [M::mem_pestat] mean and std.dev: (1220.82, 1752.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11403) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1036.285 CPU sec, 59.444 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654219, 21, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.89, 98.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 56, 2126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6344) [M::mem_pestat] mean and std.dev: (832.21, 1315.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8453) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1043.638 CPU sec, 46.389 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653910, 44, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.65, 98.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 85, 3475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10369) [M::mem_pestat] mean and std.dev: (1480.98, 2086.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13816) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1044.655 CPU sec, 60.836 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 653996, 49, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.79, 98.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 138, 2031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6043) [M::mem_pestat] mean and std.dev: (966.57, 1348.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8049) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1034.386 CPU sec, 45.845 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 654107, 44, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (360, 505, 786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1638) [M::mem_pestat] mean and std.dev: (538.70, 277.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2064) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.81, 98.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 165, 1812) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5364) [M::mem_pestat] mean and std.dev: (923.40, 1312.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7140) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1038.632 CPU sec, 59.593 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653780, 41, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.62, 98.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 51, 1687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5011) [M::mem_pestat] mean and std.dev: (955.78, 1431.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6680) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1041.118 CPU sec, 46.458 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654217, 27, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.67, 98.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 97, 1909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5653) [M::mem_pestat] mean and std.dev: (1069.37, 1624.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7567) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1042.311 CPU sec, 59.572 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654388, 38, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.51, 97.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 49, 1902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5658) [M::mem_pestat] mean and std.dev: (983.84, 1505.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7536) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1035.798 CPU sec, 46.089 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 653881, 49, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.65, 98.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 36, 592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1746) [M::mem_pestat] mean and std.dev: (171.60, 371.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2323) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1041.682 CPU sec, 61.514 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654320, 53, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.64, 98.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 37, 1148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3408) [M::mem_pestat] mean and std.dev: (241.74, 613.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4538) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1039.186 CPU sec, 46.170 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653850, 33, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.46, 98.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 192, 2366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7042) [M::mem_pestat] mean and std.dev: (998.00, 1275.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9380) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1038.692 CPU sec, 60.495 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654537, 38, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.33, 97.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 45, 956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2830) [M::mem_pestat] mean and std.dev: (301.45, 483.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3767) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1037.811 CPU sec, 46.143 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654227, 34, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.73, 97.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 58, 1444) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4292) [M::mem_pestat] mean and std.dev: (668.26, 1117.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5716) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1040.571 CPU sec, 59.683 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654444, 49, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.80, 98.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 144, 2605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7747) [M::mem_pestat] mean and std.dev: (960.59, 1436.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10318) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1033.158 CPU sec, 46.059 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654229, 41, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.59, 98.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 113, 1374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4080) [M::mem_pestat] mean and std.dev: (507.45, 777.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5433) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1041.665 CPU sec, 60.602 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654193, 32, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.59, 98.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 72, 1842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5470) [M::mem_pestat] mean and std.dev: (1164.78, 1811.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8410) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1031.236 CPU sec, 45.717 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654744, 46, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.88, 98.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 75, 2080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6186) [M::mem_pestat] mean and std.dev: (1149.96, 1730.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8239) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1036.900 CPU sec, 59.957 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654545, 37, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.55, 98.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 95, 1955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5799) [M::mem_pestat] mean and std.dev: (773.82, 1221.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7721) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1031.857 CPU sec, 46.064 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 653906, 44, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.55, 98.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 120, 2749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8187) [M::mem_pestat] mean and std.dev: (1239.21, 1642.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10906) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1044.573 CPU sec, 59.619 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654309, 36, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.93, 98.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 96, 1863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5531) [M::mem_pestat] mean and std.dev: (952.38, 1567.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7365) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1046.495 CPU sec, 46.499 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654206, 37, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.82, 98.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 68, 1703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5053) [M::mem_pestat] mean and std.dev: (921.50, 1535.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7063) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1052.459 CPU sec, 58.089 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654117, 36, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.75, 98.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 482, 2907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8665) [M::mem_pestat] mean and std.dev: (1534.75, 2012.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11544) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1058.882 CPU sec, 47.333 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 654075, 42, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.61, 98.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 36, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1020) [M::mem_pestat] mean and std.dev: (59.70, 88.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1354) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1054.953 CPU sec, 59.141 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654616, 34, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.64, 97.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 150, 2522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7514) [M::mem_pestat] mean and std.dev: (1209.12, 1695.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10010) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1050.981 CPU sec, 46.599 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654220, 44, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.75, 98.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 63, 1154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3430) [M::mem_pestat] mean and std.dev: (511.48, 862.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4568) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1049.451 CPU sec, 57.064 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653880, 46, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.85, 98.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 359, 1536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4546) [M::mem_pestat] mean and std.dev: (986.70, 1360.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6429) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1050.317 CPU sec, 46.563 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654532, 45, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.83, 97.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 71, 1823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5419) [M::mem_pestat] mean and std.dev: (607.66, 1093.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7217) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1058.535 CPU sec, 60.759 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654791, 48, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.93, 98.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 130, 2753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8213) [M::mem_pestat] mean and std.dev: (1292.21, 1909.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10943) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1062.387 CPU sec, 47.371 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654527, 41, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.67, 98.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (57, 581, 2937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8697) [M::mem_pestat] mean and std.dev: (1576.28, 2180.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11577) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1053.696 CPU sec, 58.717 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654008, 41, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.80, 98.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 97, 3058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9112) [M::mem_pestat] mean and std.dev: (1437.97, 1988.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12139) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1058.026 CPU sec, 47.061 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 654647, 39, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.76, 98.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 325, 3533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10507) [M::mem_pestat] mean and std.dev: (1586.82, 2135.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13994) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1056.878 CPU sec, 59.651 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 654229, 34, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (279, 399, 749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1689) [M::mem_pestat] mean and std.dev: (413.10, 272.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2159) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.76, 98.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 51, 1266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3764) [M::mem_pestat] mean and std.dev: (399.42, 724.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5013) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1055.442 CPU sec, 46.889 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 654179, 43, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.66, 98.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 64, 1158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3442) [M::mem_pestat] mean and std.dev: (534.71, 782.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4584) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1048.090 CPU sec, 55.018 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 653708, 49, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.50, 97.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 124, 1864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5548) [M::mem_pestat] mean and std.dev: (955.91, 1446.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7390) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1055.463 CPU sec, 47.011 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654591, 48, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.73, 98.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 51, 1542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4594) [M::mem_pestat] mean and std.dev: (637.67, 1135.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6120) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1050.652 CPU sec, 59.263 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654428, 41, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.52, 98.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 209, 1778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5268) [M::mem_pestat] mean and std.dev: (788.74, 1159.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7013) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1050.561 CPU sec, 46.692 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654458, 36, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.70, 98.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 167, 1553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4595) [M::mem_pestat] mean and std.dev: (687.86, 967.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6116) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1051.731 CPU sec, 58.478 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654857, 39, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.70, 98.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 158, 4366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13026) [M::mem_pestat] mean and std.dev: (2283.31, 2965.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17356) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1047.846 CPU sec, 46.473 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654162, 43, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.74, 98.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 138, 2578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7656) [M::mem_pestat] mean and std.dev: (1181.73, 1642.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10195) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1063.128 CPU sec, 60.012 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653716, 47, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.57, 97.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 152, 1568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4646) [M::mem_pestat] mean and std.dev: (883.20, 1302.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6185) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1055.514 CPU sec, 46.836 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654628, 29, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.83, 98.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 182, 4369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13079) [M::mem_pestat] mean and std.dev: (2106.86, 2786.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17434) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1058.951 CPU sec, 59.841 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 653586, 45, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.61, 98.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 102, 2497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7437) [M::mem_pestat] mean and std.dev: (1069.40, 1569.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9907) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1057.992 CPU sec, 47.112 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654299, 44, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.48, 98.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 215, 1739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5167) [M::mem_pestat] mean and std.dev: (920.90, 1353.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6881) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1042.344 CPU sec, 55.367 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653887, 36, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.40, 98.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 237, 2986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8910) [M::mem_pestat] mean and std.dev: (1540.43, 2386.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11872) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1053.884 CPU sec, 46.954 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654027, 45, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.71, 98.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 111, 1869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5537) [M::mem_pestat] mean and std.dev: (822.00, 1391.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7371) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1059.194 CPU sec, 59.207 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654679, 44, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.61, 98.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 111, 2766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8246) [M::mem_pestat] mean and std.dev: (1305.23, 1814.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10986) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1055.113 CPU sec, 46.801 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654041, 51, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.74, 98.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 51, 1570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4664) [M::mem_pestat] mean and std.dev: (832.94, 1474.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6730) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1056.746 CPU sec, 57.545 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 653932, 40, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.78, 98.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 62, 1866) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5556) [M::mem_pestat] mean and std.dev: (916.62, 1388.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7401) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1051.134 CPU sec, 46.614 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654217, 35, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.79, 98.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 28, 1180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3516) [M::mem_pestat] mean and std.dev: (326.35, 734.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4684) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1054.348 CPU sec, 59.746 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654070, 34, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.67, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 143, 4329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12959) [M::mem_pestat] mean and std.dev: (1896.15, 2934.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17274) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1054.223 CPU sec, 46.804 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 654326, 42, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.74, 98.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 441, 2870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8554) [M::mem_pestat] mean and std.dev: (1317.62, 1920.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11396) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1051.981 CPU sec, 59.781 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654607, 38, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.68, 98.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 32, 727) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2159) [M::mem_pestat] mean and std.dev: (239.28, 553.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2875) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1049.366 CPU sec, 46.589 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 654058, 40, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (476, 1057, 1365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3143) [M::mem_pestat] mean and std.dev: (943.27, 547.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4032) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.62, 98.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 82, 1187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3509) [M::mem_pestat] mean and std.dev: (321.91, 652.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4670) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1053.129 CPU sec, 57.341 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654672, 35, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.77, 98.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 113, 1788) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5318) [M::mem_pestat] mean and std.dev: (765.68, 1003.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7083) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1053.285 CPU sec, 46.802 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 654103, 35, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.78, 98.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 119, 1170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3456) [M::mem_pestat] mean and std.dev: (545.58, 756.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4599) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1060.989 CPU sec, 60.004 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654417, 43, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.92, 98.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 179, 1998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5912) [M::mem_pestat] mean and std.dev: (881.12, 1331.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7869) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1050.790 CPU sec, 46.739 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654394, 40, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.71, 98.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 54, 1771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5267) [M::mem_pestat] mean and std.dev: (694.94, 1347.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7015) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1062.257 CPU sec, 61.340 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 653929, 36, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.67, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 67, 2209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6597) [M::mem_pestat] mean and std.dev: (1015.40, 1519.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8791) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1053.787 CPU sec, 46.701 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654088, 34, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.56, 98.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 69, 1122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3328) [M::mem_pestat] mean and std.dev: (371.47, 719.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4431) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1055.713 CPU sec, 60.531 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 653862, 43, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 341, 425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (284.12, 100.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.69, 98.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 53, 2334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6960) [M::mem_pestat] mean and std.dev: (1098.40, 1625.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9273) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1055.968 CPU sec, 46.892 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654537, 48, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.58, 98.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 167, 3128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9340) [M::mem_pestat] mean and std.dev: (1654.21, 2463.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12446) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1061.139 CPU sec, 61.247 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 654044, 31, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.84, 98.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 144, 1864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5548) [M::mem_pestat] mean and std.dev: (871.63, 1432.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7390) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1051.702 CPU sec, 46.599 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654651, 28, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.40, 98.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 52, 2846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8510) [M::mem_pestat] mean and std.dev: (1143.50, 1661.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11342) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1054.805 CPU sec, 60.688 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654241, 41, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.67, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 169, 2635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7833) [M::mem_pestat] mean and std.dev: (1428.71, 1994.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10432) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1048.722 CPU sec, 46.664 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654688, 47, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.52, 98.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 46, 867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2565) [M::mem_pestat] mean and std.dev: (242.64, 567.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3414) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1054.392 CPU sec, 61.618 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 654022, 41, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (385, 514, 814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1672) [M::mem_pestat] mean and std.dev: (658.50, 467.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2530) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.61, 98.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 176, 4358) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13020) [M::mem_pestat] mean and std.dev: (2136.24, 3157.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17351) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1050.957 CPU sec, 46.635 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654380, 37, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.60, 98.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 1589, 4031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12037) [M::mem_pestat] mean and std.dev: (1908.41, 2257.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16040) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1063.368 CPU sec, 61.319 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653992, 46, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.35, 97.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 153, 4370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13036) [M::mem_pestat] mean and std.dev: (1984.00, 2733.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17369) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1050.729 CPU sec, 46.482 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 654439, 53, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.52, 98.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 55, 742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2188) [M::mem_pestat] mean and std.dev: (236.33, 464.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2911) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1045.959 CPU sec, 55.070 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653759, 39, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.60, 97.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 55, 2441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7291) [M::mem_pestat] mean and std.dev: (1105.00, 1731.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9716) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1054.858 CPU sec, 46.656 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 654238, 41, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (295, 409, 871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2023) [M::mem_pestat] mean and std.dev: (580.09, 434.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2599) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.39, 97.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 127, 2972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8858) [M::mem_pestat] mean and std.dev: (1670.83, 2578.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11983) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1056.974 CPU sec, 60.813 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654151, 47, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.63, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 64, 990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2910) [M::mem_pestat] mean and std.dev: (273.05, 538.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3870) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1050.351 CPU sec, 46.616 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653949, 41, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.69, 98.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 96, 1535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4567) [M::mem_pestat] mean and std.dev: (724.44, 1196.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6083) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1061.847 CPU sec, 60.020 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 653925, 36, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.70, 98.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 79, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2144) [M::mem_pestat] mean and std.dev: (313.12, 579.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2848) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1053.075 CPU sec, 46.572 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654736, 37, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.64, 98.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 80, 1360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4020) [M::mem_pestat] mean and std.dev: (239.10, 456.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5350) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1057.938 CPU sec, 61.151 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 653664, 32, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.55, 98.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 62, 1060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3138) [M::mem_pestat] mean and std.dev: (515.48, 873.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4177) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (378, 1553, 1969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5151) [M::mem_pestat] mean and std.dev: (934.38, 715.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6742) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1059.963 CPU sec, 46.987 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 653722, 49, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.53, 97.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 70, 1872) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5574) [M::mem_pestat] mean and std.dev: (760.04, 1282.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7425) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1054.696 CPU sec, 60.745 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 654384, 48, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.65, 98.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 194, 2821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8417) [M::mem_pestat] mean and std.dev: (1116.59, 1867.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11215) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1054.799 CPU sec, 46.845 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 653571, 35, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.53, 98.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 79, 2581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7717) [M::mem_pestat] mean and std.dev: (1175.54, 1804.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10285) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1046.232 CPU sec, 55.564 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654144, 51, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.53, 98.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 87, 1976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5888) [M::mem_pestat] mean and std.dev: (1071.80, 1672.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7844) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1054.725 CPU sec, 46.823 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 654049, 41, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.66, 98.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 79, 1699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5071) [M::mem_pestat] mean and std.dev: (922.38, 1437.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6757) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1057.480 CPU sec, 58.959 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654009, 50, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.75, 98.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 102, 2049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6121) [M::mem_pestat] mean and std.dev: (1004.96, 1549.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8157) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1053.514 CPU sec, 46.753 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654086, 38, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.64, 98.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 105, 2594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7726) [M::mem_pestat] mean and std.dev: (1172.70, 1788.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10292) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1055.945 CPU sec, 60.608 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654111, 44, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.41, 98.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 67, 2573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7675) [M::mem_pestat] mean and std.dev: (999.00, 1511.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10226) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1052.591 CPU sec, 46.667 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654261, 46, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.73, 98.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 93, 2190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6520) [M::mem_pestat] mean and std.dev: (1112.40, 1809.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8685) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1056.984 CPU sec, 60.671 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654096, 35, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.61, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 202, 1890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5582) [M::mem_pestat] mean and std.dev: (1174.21, 1622.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7663) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1048.707 CPU sec, 46.421 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654469, 37, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.78, 98.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 47, 1817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5401) [M::mem_pestat] mean and std.dev: (748.65, 1426.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7193) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1054.837 CPU sec, 61.190 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654044, 62, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.62, 97.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (49, 701, 2623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7771) [M::mem_pestat] mean and std.dev: (1125.85, 1404.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10345) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1047.585 CPU sec, 46.383 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654294, 50, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.82, 98.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 79, 2958) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8804) [M::mem_pestat] mean and std.dev: (1438.58, 2054.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11727) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1058.741 CPU sec, 60.551 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 654365, 36, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.66, 98.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 176, 2636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7874) [M::mem_pestat] mean and std.dev: (1384.77, 1885.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10493) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1036.006 CPU sec, 45.687 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654271, 41, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.53, 98.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 131, 1905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5651) [M::mem_pestat] mean and std.dev: (953.25, 1465.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7524) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1054.433 CPU sec, 59.910 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654203, 48, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.64, 98.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 63, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1427) [M::mem_pestat] mean and std.dev: (92.65, 114.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1894) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1053.003 CPU sec, 46.625 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 654131, 55, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (335, 653, 1379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3467) [M::mem_pestat] mean and std.dev: (753.22, 463.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4511) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.55, 97.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 64, 1454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4318) [M::mem_pestat] mean and std.dev: (567.90, 983.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5750) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1055.079 CPU sec, 58.728 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 654242, 47, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (420, 1483, 2510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6690) [M::mem_pestat] mean and std.dev: (1671.78, 1712.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.70, 98.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 527, 2846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8438) [M::mem_pestat] mean and std.dev: (1473.30, 1904.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11234) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1045.146 CPU sec, 46.320 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 653889, 48, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.75, 98.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 83, 3224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9616) [M::mem_pestat] mean and std.dev: (1415.04, 2022.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12812) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1046.688 CPU sec, 59.317 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654587, 41, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.66, 98.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 52, 2594) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7738) [M::mem_pestat] mean and std.dev: (1156.42, 1905.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10310) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1049.239 CPU sec, 46.625 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654355, 46, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.84, 98.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 74, 1878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5602) [M::mem_pestat] mean and std.dev: (762.19, 1280.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7464) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1056.849 CPU sec, 60.111 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654340, 39, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.62, 98.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 48, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1728) [M::mem_pestat] mean and std.dev: (251.61, 427.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2297) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1051.342 CPU sec, 46.663 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 653809, 47, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.79, 98.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 76, 1387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4119) [M::mem_pestat] mean and std.dev: (560.00, 861.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5485) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1058.035 CPU sec, 60.846 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 653835, 45, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.67, 98.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 36, 860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2542) [M::mem_pestat] mean and std.dev: (198.68, 469.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3383) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1049.144 CPU sec, 46.534 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654634, 40, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.37, 97.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 146, 1768) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5230) [M::mem_pestat] mean and std.dev: (969.74, 1388.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6961) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1051.408 CPU sec, 60.801 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 654076, 52, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.66, 98.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (8, 42, 1662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4970) [M::mem_pestat] mean and std.dev: (628.09, 1317.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6624) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1041.152 CPU sec, 46.058 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 654178, 33, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.57, 97.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 22, 1522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4542) [M::mem_pestat] mean and std.dev: (393.77, 705.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6052) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1052.034 CPU sec, 59.905 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654064, 53, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.55, 97.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 54, 1162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3438) [M::mem_pestat] mean and std.dev: (363.76, 678.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4576) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1041.922 CPU sec, 46.091 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 654053, 38, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.61, 98.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 1821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5433) [M::mem_pestat] mean and std.dev: (677.44, 1254.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7239) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1046.682 CPU sec, 59.080 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 653681, 46, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.76, 98.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 92, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1651) [M::mem_pestat] mean and std.dev: (109.81, 161.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2195) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1044.536 CPU sec, 46.361 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654551, 35, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.67, 98.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 45, 1199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3537) [M::mem_pestat] mean and std.dev: (320.37, 626.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4706) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1048.810 CPU sec, 60.514 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654268, 50, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.70, 98.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 46, 1354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4022) [M::mem_pestat] mean and std.dev: (466.47, 1045.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5356) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1042.601 CPU sec, 46.164 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654433, 47, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.80, 98.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 73, 2936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8762) [M::mem_pestat] mean and std.dev: (1630.85, 2708.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12465) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1049.304 CPU sec, 59.239 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654401, 39, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.69, 98.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 55, 1766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5258) [M::mem_pestat] mean and std.dev: (539.56, 1078.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7004) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1045.543 CPU sec, 46.526 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654626, 42, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.40, 97.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 118, 3543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10575) [M::mem_pestat] mean and std.dev: (1602.95, 2340.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14091) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1040.266 CPU sec, 60.686 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654307, 40, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.61, 97.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 41, 2347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6995) [M::mem_pestat] mean and std.dev: (1171.95, 1858.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9319) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1038.083 CPU sec, 45.972 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 653845, 45, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.49, 98.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 59, 1373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4081) [M::mem_pestat] mean and std.dev: (561.81, 925.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5435) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1050.866 CPU sec, 60.796 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654028, 51, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.63, 98.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 97, 1345) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3987) [M::mem_pestat] mean and std.dev: (608.65, 979.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5308) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1037.204 CPU sec, 45.939 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653883, 45, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.40, 98.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 71, 524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1522) [M::mem_pestat] mean and std.dev: (169.63, 291.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2021) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1046.103 CPU sec, 59.739 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 653395, 36, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.47, 97.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 101, 1847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5469) [M::mem_pestat] mean and std.dev: (687.61, 1205.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7280) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1039.479 CPU sec, 46.181 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 654146, 39, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.40, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 67, 1869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5563) [M::mem_pestat] mean and std.dev: (832.73, 1437.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7410) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1039.149 CPU sec, 58.935 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654035, 47, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.71, 98.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 299, 2640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7860) [M::mem_pestat] mean and std.dev: (1267.86, 1876.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10470) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1035.514 CPU sec, 45.999 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654247, 34, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.72, 98.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 102, 847) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2501) [M::mem_pestat] mean and std.dev: (262.71, 482.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3328) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1042.866 CPU sec, 59.394 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654227, 33, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.58, 98.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 38, 1031) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3063) [M::mem_pestat] mean and std.dev: (454.77, 798.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4079) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1037.803 CPU sec, 45.984 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653842, 40, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.60, 98.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 133, 4349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13017) [M::mem_pestat] mean and std.dev: (1710.15, 2429.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17351) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1044.890 CPU sec, 59.876 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654263, 44, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.70, 98.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 61, 1540) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4570) [M::mem_pestat] mean and std.dev: (663.50, 1154.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6085) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1038.510 CPU sec, 45.991 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654208, 44, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.75, 98.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 427, 2651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7883) [M::mem_pestat] mean and std.dev: (1366.60, 1741.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10499) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1041.687 CPU sec, 59.798 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 653488, 42, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.66, 98.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 158, 3192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9532) [M::mem_pestat] mean and std.dev: (1611.05, 2094.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12702) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1038.759 CPU sec, 45.966 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654768, 47, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.79, 98.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 51, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (68.21, 74.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1038.719 CPU sec, 59.280 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654214, 40, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.80, 98.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 209, 2595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7721) [M::mem_pestat] mean and std.dev: (1229.10, 1669.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10284) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1037.642 CPU sec, 46.050 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 654209, 37, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.63, 98.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 40, 2111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6297) [M::mem_pestat] mean and std.dev: (911.91, 1418.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8390) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1042.681 CPU sec, 58.738 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654103, 48, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.52, 98.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 86, 1485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4411) [M::mem_pestat] mean and std.dev: (808.41, 1337.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6159) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1042.338 CPU sec, 46.279 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 653625, 46, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.42, 97.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 43, 2625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7841) [M::mem_pestat] mean and std.dev: (1038.67, 1986.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10449) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1036.421 CPU sec, 56.871 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654421, 31, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.75, 98.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 538, 4399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13141) [M::mem_pestat] mean and std.dev: (2308.39, 2770.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17512) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1038.148 CPU sec, 46.230 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 653696, 34, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.43, 98.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 112, 2327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6941) [M::mem_pestat] mean and std.dev: (706.24, 1196.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9248) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1039.693 CPU sec, 57.666 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654735, 43, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.70, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 133, 3301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9853) [M::mem_pestat] mean and std.dev: (1730.58, 2523.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13129) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1042.130 CPU sec, 46.239 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654280, 42, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.80, 98.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 131, 2133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6371) [M::mem_pestat] mean and std.dev: (1039.10, 1479.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8490) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1046.449 CPU sec, 59.223 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654252, 39, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.89, 98.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 80, 1573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4681) [M::mem_pestat] mean and std.dev: (586.35, 1092.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6235) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1032.297 CPU sec, 45.645 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654117, 40, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.68, 98.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 101, 2324) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6912) [M::mem_pestat] mean and std.dev: (873.46, 1371.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9206) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1042.522 CPU sec, 57.376 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653609, 39, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.45, 97.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 113, 1586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4714) [M::mem_pestat] mean and std.dev: (770.94, 1325.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6278) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1040.932 CPU sec, 46.067 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 654618, 42, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.57, 98.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 253, 3042) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9082) [M::mem_pestat] mean and std.dev: (1660.29, 2296.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12102) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1038.410 CPU sec, 58.247 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653809, 56, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.50, 97.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 102, 1778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5278) [M::mem_pestat] mean and std.dev: (990.21, 1515.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7052) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1043.936 CPU sec, 46.272 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653829, 48, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.49, 98.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 103, 2197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6549) [M::mem_pestat] mean and std.dev: (1052.26, 1597.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8725) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1044.389 CPU sec, 58.697 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654272, 45, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.59, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 51, 878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2576) [M::mem_pestat] mean and std.dev: (212.74, 406.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3425) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1037.940 CPU sec, 46.092 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654491, 38, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.40, 98.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 145, 1473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4361) [M::mem_pestat] mean and std.dev: (585.58, 1065.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5805) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1040.783 CPU sec, 59.397 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653762, 38, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.83, 98.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 36, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (36.24, 38.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1032.663 CPU sec, 45.750 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 653322, 35, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.49, 98.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 63, 1731) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5139) [M::mem_pestat] mean and std.dev: (990.12, 1427.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6843) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1049.931 CPU sec, 58.634 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 653831, 48, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.81, 98.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 46, 306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 870) [M::mem_pestat] mean and std.dev: (94.20, 149.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1036.867 CPU sec, 45.946 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 653717, 38, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.74, 98.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 82, 2913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8709) [M::mem_pestat] mean and std.dev: (1591.58, 2195.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11607) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1037.816 CPU sec, 57.185 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654567, 39, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.52, 97.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 61, 1810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5396) [M::mem_pestat] mean and std.dev: (845.00, 1442.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7189) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1032.367 CPU sec, 45.734 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654756, 40, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.37, 98.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 1019, 4366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13004) [M::mem_pestat] mean and std.dev: (2304.82, 2827.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17323) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1038.400 CPU sec, 57.213 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654562, 41, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.37, 98.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 51, 709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2081) [M::mem_pestat] mean and std.dev: (122.06, 217.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2767) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1033.215 CPU sec, 46.211 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 653476, 48, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.76, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 57, 1488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4406) [M::mem_pestat] mean and std.dev: (685.33, 1234.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5865) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1042.092 CPU sec, 59.468 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654160, 41, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.68, 98.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 43, 1828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5456) [M::mem_pestat] mean and std.dev: (764.59, 1376.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7270) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1034.334 CPU sec, 45.796 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654150, 33, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.66, 98.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 75, 2032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6060) [M::mem_pestat] mean and std.dev: (949.19, 1343.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8074) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1046.756 CPU sec, 59.080 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654723, 48, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.56, 98.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 59, 3034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9066) [M::mem_pestat] mean and std.dev: (1445.02, 2234.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12082) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1029.876 CPU sec, 45.627 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 653699, 55, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.73, 98.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 65, 1799) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5339) [M::mem_pestat] mean and std.dev: (808.62, 1370.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7109) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1035.884 CPU sec, 57.059 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 653912, 40, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.42, 98.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 36, 1081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3211) [M::mem_pestat] mean and std.dev: (423.47, 867.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4276) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1029.282 CPU sec, 45.702 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654009, 40, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.45, 97.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 75, 1454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4332) [M::mem_pestat] mean and std.dev: (260.81, 485.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5771) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1043.132 CPU sec, 58.017 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 653665, 32, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (275, 888, 1183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2999) [M::mem_pestat] mean and std.dev: (750.40, 481.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3907) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.67, 98.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 324, 3034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9036) [M::mem_pestat] mean and std.dev: (1543.34, 2123.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12037) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1036.183 CPU sec, 45.793 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 654085, 46, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.78, 98.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 88, 2388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7116) [M::mem_pestat] mean and std.dev: (1185.82, 1658.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9480) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1034.783 CPU sec, 57.682 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 653847, 57, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.43, 97.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 247, 3249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9693) [M::mem_pestat] mean and std.dev: (1438.26, 2059.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12915) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1036.877 CPU sec, 45.975 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654592, 40, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.71, 98.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 46, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (70.47, 115.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 966) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1039.015 CPU sec, 59.926 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 654267, 60, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.60, 98.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 75, 1709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5093) [M::mem_pestat] mean and std.dev: (882.53, 1439.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6785) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1028.055 CPU sec, 45.592 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654240, 40, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.64, 98.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 89, 2892) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8634) [M::mem_pestat] mean and std.dev: (1330.67, 2081.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11505) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1028.573 CPU sec, 56.971 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654389, 43, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.44, 98.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 116, 2350) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7000) [M::mem_pestat] mean and std.dev: (994.17, 1630.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9325) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1036.054 CPU sec, 46.286 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654614, 40, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.63, 97.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 42, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1363) [M::mem_pestat] mean and std.dev: (145.59, 245.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1810) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1018.944 CPU sec, 52.924 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654272, 46, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.43, 98.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 46, 699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2057) [M::mem_pestat] mean and std.dev: (234.95, 483.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2736) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1022.552 CPU sec, 45.241 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654823, 45, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.63, 98.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 83, 1760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5234) [M::mem_pestat] mean and std.dev: (841.36, 1338.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6971) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1020.575 CPU sec, 54.675 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654624, 50, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.79, 98.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 94, 1695) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5021) [M::mem_pestat] mean and std.dev: (884.62, 1432.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6684) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1019.899 CPU sec, 45.217 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654682, 42, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.61, 98.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 42, 998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2952) [M::mem_pestat] mean and std.dev: (313.97, 638.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3929) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1029.691 CPU sec, 56.694 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 653701, 43, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.58, 98.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 84, 1379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4069) [M::mem_pestat] mean and std.dev: (462.89, 800.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5414) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1024.734 CPU sec, 45.448 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654022, 53, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.63, 98.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 67, 1760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5246) [M::mem_pestat] mean and std.dev: (764.87, 1451.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6989) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1039.257 CPU sec, 58.495 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654418, 43, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.67, 98.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 106, 2909) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8665) [M::mem_pestat] mean and std.dev: (1459.65, 2182.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11543) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1029.026 CPU sec, 45.630 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654823, 43, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.55, 98.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 109, 1842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5448) [M::mem_pestat] mean and std.dev: (882.76, 1441.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7251) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1036.432 CPU sec, 59.259 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 653916, 36, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.72, 98.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 78, 3257) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9741) [M::mem_pestat] mean and std.dev: (1632.94, 2392.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12983) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1035.328 CPU sec, 45.907 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654086, 47, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.70, 98.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 103, 1506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4478) [M::mem_pestat] mean and std.dev: (633.73, 1133.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5964) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1035.103 CPU sec, 57.697 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654257, 42, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.67, 98.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 605, 4391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13093) [M::mem_pestat] mean and std.dev: (2555.90, 3267.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17444) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1031.136 CPU sec, 55.945 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654030, 48, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.42, 98.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 48, 1355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4033) [M::mem_pestat] mean and std.dev: (623.83, 1053.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5372) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1028.872 CPU sec, 45.609 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 654521, 42, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.77, 98.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 56, 1977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5891) [M::mem_pestat] mean and std.dev: (953.64, 1565.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7848) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1040.803 CPU sec, 59.604 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 653565, 49, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.77, 98.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 48, 1675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4993) [M::mem_pestat] mean and std.dev: (614.35, 1115.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6652) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1033.117 CPU sec, 45.767 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 653778, 29, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 415, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1393) [M::mem_pestat] mean and std.dev: (345.00, 153.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.45, 98.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 72, 2334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6956) [M::mem_pestat] mean and std.dev: (731.00, 1060.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9267) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1038.550 CPU sec, 59.853 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 654235, 42, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.65, 98.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 152, 1702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5048) [M::mem_pestat] mean and std.dev: (1112.00, 1572.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7402) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1031.572 CPU sec, 45.768 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654109, 31, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.74, 97.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 181, 2565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7649) [M::mem_pestat] mean and std.dev: (1362.29, 1671.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10191) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1037.381 CPU sec, 59.447 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 654309, 37, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.88, 98.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 64, 1954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5826) [M::mem_pestat] mean and std.dev: (1007.46, 1473.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7762) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1035.337 CPU sec, 46.226 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 654651, 33, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.60, 98.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (40, 170, 1912) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5656) [M::mem_pestat] mean and std.dev: (854.69, 1098.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7528) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1042.798 CPU sec, 60.103 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 654253, 36, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.88, 98.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 73, 1896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5630) [M::mem_pestat] mean and std.dev: (846.71, 1346.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7497) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1030.036 CPU sec, 45.617 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 654526, 31, 0) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 764) [M::mem_pestat] mean and std.dev: (446.53, 97.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 892) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 56, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 333) [M::mem_pestat] mean and std.dev: (55.38, 46.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 438) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1032.694 CPU sec, 58.629 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 654756, 31, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 439, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.68, 98.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 109, 1158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3426) [M::mem_pestat] mean and std.dev: (361.27, 613.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4560) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1030.730 CPU sec, 45.720 real sec [M::process] read 1534034 sequences (231639134 bp)... [M::process] 0 single-end sequences; 1534034 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 630999, 47, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (380, 440, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 767) [M::mem_pestat] mean and std.dev: (446.85, 98.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 896) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 105, 1702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5056) [M::mem_pestat] mean and std.dev: (844.86, 1480.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6767) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1534034 reads in 985.280 CPU sec, 41.490 real sec [main] Version: 0.7.17-r1188 [main] CMD: /home/csyk2proj/tools/bwa.kit/bwa mem -p -t24 -R@RG\tID:KPGP-00337\tPL:ILLUMINA\tSM:KPGP-00337 -C /home/csyk2proj/data/bwa.kit/hs38DH.fa - [main] Real time: 38669.967 sec; CPU: 461823.135 sec