[M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666938, 46, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 299, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (4, 544) [M::mem_pestat] mean and std.dev: (256.73, 75.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.58, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 37, 66) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 168) [M::mem_pestat] mean and std.dev: (37.67, 29.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 219) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1069.326 CPU sec, 60.708 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666479, 64, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (282, 493, 1264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3228) [M::mem_pestat] mean and std.dev: (716.40, 513.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4210) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.60, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 53, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 618) [M::mem_pestat] mean and std.dev: (83.28, 125.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1059.754 CPU sec, 46.966 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666598, 48, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 606, 3891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11227) [M::mem_pestat] mean and std.dev: (2296.70, 3202.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15107) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.52, 82.90) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 55, 1267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3759) [M::mem_pestat] mean and std.dev: (545.91, 969.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5005) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1058.676 CPU sec, 54.816 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666885, 45, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.44, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 27, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1334) [M::mem_pestat] mean and std.dev: (117.97, 259.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1773) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1054.502 CPU sec, 46.412 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666983, 49, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 198, 1132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3074) [M::mem_pestat] mean and std.dev: (590.00, 804.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4045) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.34, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 44, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1430) [M::mem_pestat] mean and std.dev: (138.44, 245.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1900) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1057.848 CPU sec, 57.568 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666765, 48, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.65, 82.89) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 40, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (79.74, 133.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1054.035 CPU sec, 46.776 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666620, 42, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.53, 82.94) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 41, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 239) [M::mem_pestat] mean and std.dev: (39.62, 38.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 312) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1057.443 CPU sec, 57.216 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666768, 48, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.62, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 36, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084) [M::mem_pestat] mean and std.dev: (63.00, 87.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1441) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1056.188 CPU sec, 46.649 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666453, 39, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 268, 403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 685) [M::mem_pestat] mean and std.dev: (278.27, 51.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 826) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.39, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 45, 1199) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3551) [M::mem_pestat] mean and std.dev: (298.79, 579.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4727) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1055.278 CPU sec, 57.408 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667142, 53, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (300, 635, 1037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2511) [M::mem_pestat] mean and std.dev: (731.60, 526.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3248) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.30, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 37, 399) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1161) [M::mem_pestat] mean and std.dev: (100.67, 227.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1542) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1065.756 CPU sec, 47.125 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666342, 49, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.35, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 60, 2011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5995) [M::mem_pestat] mean and std.dev: (793.18, 1405.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7987) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1056.920 CPU sec, 52.519 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666585, 44, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 303, 728) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1942) [M::mem_pestat] mean and std.dev: (361.00, 324.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2549) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.48, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 43, 1130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3350) [M::mem_pestat] mean and std.dev: (226.32, 496.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4460) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1063.765 CPU sec, 59.147 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666730, 58, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.63, 82.90) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 44, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 702) [M::mem_pestat] mean and std.dev: (48.98, 58.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (680, 1099, 2901) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7343) [M::mem_pestat] mean and std.dev: (1608.10, 1302.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9564) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1060.453 CPU sec, 46.827 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666473, 49, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 501, 1409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3849) [M::mem_pestat] mean and std.dev: (573.86, 507.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5069) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.46, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 41, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1639) [M::mem_pestat] mean and std.dev: (147.34, 329.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2179) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1056.684 CPU sec, 59.825 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666931, 44, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 320, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1219) [M::mem_pestat] mean and std.dev: (311.89, 156.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1557) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.58, 82.95) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 43, 951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2807) [M::mem_pestat] mean and std.dev: (379.64, 791.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3735) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1050.371 CPU sec, 46.521 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666895, 51, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.56, 82.90) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 31, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 236) [M::mem_pestat] mean and std.dev: (28.55, 23.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 309) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (251, 735, 1671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4511) [M::mem_pestat] mean and std.dev: (940.90, 878.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5931) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1049.453 CPU sec, 59.333 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666668, 48, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.25, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 41, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (61.95, 80.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1049.494 CPU sec, 46.474 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666595, 43, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.49, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 82, 521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1513) [M::mem_pestat] mean and std.dev: (223.70, 365.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2009) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1048.975 CPU sec, 58.277 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666625, 48, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 1049, 2406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6634) [M::mem_pestat] mean and std.dev: (937.00, 882.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.54, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 26, 260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 748) [M::mem_pestat] mean and std.dev: (34.78, 45.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 992) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.339 CPU sec, 46.051 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666653, 53, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.41, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 30, 75) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 195) [M::mem_pestat] mean and std.dev: (33.57, 35.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 255) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1048.123 CPU sec, 57.746 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666829, 59, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (365, 535, 1307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3191) [M::mem_pestat] mean and std.dev: (842.62, 748.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4133) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.63, 82.97) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 36, 1142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3380) [M::mem_pestat] mean and std.dev: (196.57, 408.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4499) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.337 CPU sec, 46.120 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 667539, 61, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (132, 408, 1108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3060) [M::mem_pestat] mean and std.dev: (527.57, 711.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4036) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.62, 82.96) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 47, 508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1486) [M::mem_pestat] mean and std.dev: (164.40, 316.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1975) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (708, 1106, 1589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3351) [M::mem_pestat] mean and std.dev: (1188.18, 733.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4232) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1047.268 CPU sec, 56.332 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666689, 60, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.51, 82.96) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 47, 803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2371) [M::mem_pestat] mean and std.dev: (246.19, 437.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3155) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.357 CPU sec, 56.337 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666475, 64, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.42, 82.85) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 39, 773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2295) [M::mem_pestat] mean and std.dev: (196.91, 410.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3056) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1046.037 CPU sec, 46.254 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 667468, 49, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.58, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 34, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (34.15, 33.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 410) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1045.027 CPU sec, 57.044 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667222, 61, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.28, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 33, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (69.04, 142.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1199) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1048.814 CPU sec, 46.425 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666691, 55, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.50, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 44, 827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2443) [M::mem_pestat] mean and std.dev: (263.12, 475.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3251) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1046.797 CPU sec, 57.957 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 667009, 46, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (323, 847, 1464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3746) [M::mem_pestat] mean and std.dev: (796.38, 606.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4887) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.44, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 53, 770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2262) [M::mem_pestat] mean and std.dev: (163.70, 399.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3008) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1044.491 CPU sec, 46.225 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666538, 59, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 292, 755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1771) [M::mem_pestat] mean and std.dev: (400.18, 277.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2279) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.51, 82.95) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 35, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (44.85, 60.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1051.447 CPU sec, 58.404 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666960, 36, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 337, 2132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5968) [M::mem_pestat] mean and std.dev: (1046.41, 1451.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7886) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.45, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 35, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (58.14, 87.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1165) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.369 CPU sec, 45.975 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666404, 54, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 365, 837) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2219) [M::mem_pestat] mean and std.dev: (540.93, 534.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2910) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.54, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 48, 376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1080) [M::mem_pestat] mean and std.dev: (103.40, 183.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1432) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.880 CPU sec, 57.312 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666863, 63, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.36, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 30, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 194) [M::mem_pestat] mean and std.dev: (31.53, 28.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 253) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (303, 1013, 1185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2949) [M::mem_pestat] mean and std.dev: (782.80, 470.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3831) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1045.588 CPU sec, 46.382 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666533, 50, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 854, 1894) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5180) [M::mem_pestat] mean and std.dev: (903.45, 937.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.58, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 37, 1708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5092) [M::mem_pestat] mean and std.dev: (526.07, 981.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6784) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1149, 1554, 2441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5025) [M::mem_pestat] mean and std.dev: (1602.70, 902.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1053.710 CPU sec, 59.392 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666897, 48, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 313, 862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2060) [M::mem_pestat] mean and std.dev: (548.43, 463.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2659) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.49, 82.98) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 39, 930) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2758) [M::mem_pestat] mean and std.dev: (250.49, 615.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3672) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1048.857 CPU sec, 58.893 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667650, 48, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 411, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3113) [M::mem_pestat] mean and std.dev: (499.70, 445.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4091) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.42, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 45, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 312) [M::mem_pestat] mean and std.dev: (43.79, 34.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.476 CPU sec, 45.932 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666266, 56, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (304, 699, 1126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2770) [M::mem_pestat] mean and std.dev: (739.00, 594.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3592) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.30, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 42, 483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1407) [M::mem_pestat] mean and std.dev: (143.04, 253.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1869) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.204 CPU sec, 57.283 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666859, 40, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.55, 82.91) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 54, 312) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 892) [M::mem_pestat] mean and std.dev: (72.45, 96.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1182) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1045.758 CPU sec, 46.362 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 666661, 40, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.59, 82.98) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 41, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 464) [M::mem_pestat] mean and std.dev: (39.58, 38.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 613) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.137 CPU sec, 57.503 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666977, 55, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 328, 556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1278) [M::mem_pestat] mean and std.dev: (275.90, 154.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.34, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 28, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 402) [M::mem_pestat] mean and std.dev: (31.79, 36.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 531) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.467 CPU sec, 46.043 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666707, 51, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 312, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (243.67, 99.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.41, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 36, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (41.92, 41.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 546) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1045.547 CPU sec, 58.547 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666876, 38, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.67, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 28, 67) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 167) [M::mem_pestat] mean and std.dev: (28.47, 19.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 217) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.148 CPU sec, 46.045 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666655, 57, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (68, 286, 862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2450) [M::mem_pestat] mean and std.dev: (360.00, 375.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3244) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.52, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 55, 1845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5491) [M::mem_pestat] mean and std.dev: (795.22, 1303.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7314) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1045.113 CPU sec, 58.316 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667058, 50, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (112, 176, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 787) [M::mem_pestat] mean and std.dev: (188.10, 111.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1012) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.49, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 38, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (37.72, 48.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 590) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.704 CPU sec, 46.092 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666963, 51, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (188, 448, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2630) [M::mem_pestat] mean and std.dev: (493.80, 430.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3444) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.50, 82.92) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 37, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 283) [M::mem_pestat] mean and std.dev: (35.12, 32.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 373) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.008 CPU sec, 59.097 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666959, 50, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (138, 242, 1279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3561) [M::mem_pestat] mean and std.dev: (621.27, 668.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.51, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 40, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (41.37, 43.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 452) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1035.572 CPU sec, 57.060 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666759, 46, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (321, 903, 1096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2646) [M::mem_pestat] mean and std.dev: (737.89, 567.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3421) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.45, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 47, 929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2745) [M::mem_pestat] mean and std.dev: (211.29, 509.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3653) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1373, 1996, 3279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7091) [M::mem_pestat] mean and std.dev: (2260.27, 1527.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8997) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1040.869 CPU sec, 45.937 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666589, 43, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.39, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 38, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (46.59, 61.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 575) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1040.840 CPU sec, 57.868 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667643, 42, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (45, 564, 1373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4029) [M::mem_pestat] mean and std.dev: (634.56, 626.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5357) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.46, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 30, 60) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 154) [M::mem_pestat] mean and std.dev: (29.62, 25.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 201) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1032.168 CPU sec, 45.766 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 666733, 43, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.42, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 52, 1873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5579) [M::mem_pestat] mean and std.dev: (760.79, 1308.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7432) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1039.831 CPU sec, 57.982 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 665945, 42, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 350, 1090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2928) [M::mem_pestat] mean and std.dev: (672.35, 746.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3847) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.42, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 48, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (53.59, 57.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1013) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.670 CPU sec, 46.300 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666013, 47, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.51, 82.93) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 52, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (55.94, 72.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1292) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.359 CPU sec, 56.798 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666355, 59, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.59, 82.98) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 40, 1153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3433) [M::mem_pestat] mean and std.dev: (151.98, 382.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4573) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.960 CPU sec, 45.997 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666883, 50, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.47, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 51, 797) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2349) [M::mem_pestat] mean and std.dev: (191.88, 369.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3125) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.730 CPU sec, 57.829 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666276, 57, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 371, 562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1158) [M::mem_pestat] mean and std.dev: (280.11, 182.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1456) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.48, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 40, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 866) [M::mem_pestat] mean and std.dev: (87.26, 154.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1148) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.278 CPU sec, 46.209 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666969, 63, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.35, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 43, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 650) [M::mem_pestat] mean and std.dev: (66.53, 114.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 861) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.088 CPU sec, 58.581 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666834, 46, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.58, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 45, 205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (44.66, 45.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 763) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.846 CPU sec, 46.189 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666286, 48, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 384, 1224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3224) [M::mem_pestat] mean and std.dev: (769.80, 863.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4224) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.62, 82.94) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 44, 322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (75.82, 152.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1255) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1042.722 CPU sec, 57.740 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 667181, 56, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.46, 82.90) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 36, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 503) [M::mem_pestat] mean and std.dev: (34.21, 32.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1032.488 CPU sec, 45.667 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666326, 48, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 356, 996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2552) [M::mem_pestat] mean and std.dev: (507.67, 524.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3330) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.33, 82.86) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 70, 1348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3984) [M::mem_pestat] mean and std.dev: (521.63, 979.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5302) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1045.619 CPU sec, 56.612 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666889, 50, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.45, 83.00) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 39, 1263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3753) [M::mem_pestat] mean and std.dev: (516.93, 976.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4998) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1046.218 CPU sec, 46.442 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666730, 61, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (301, 425, 1682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4444) [M::mem_pestat] mean and std.dev: (593.67, 547.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5825) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.51, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 42, 1839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5483) [M::mem_pestat] mean and std.dev: (597.93, 1259.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7305) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1043.974 CPU sec, 57.971 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666567, 49, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (356, 770, 1393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3467) [M::mem_pestat] mean and std.dev: (746.40, 626.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4504) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.47, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 38, 78) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 190) [M::mem_pestat] mean and std.dev: (34.03, 25.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 246) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1046.415 CPU sec, 46.486 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 667346, 44, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.52, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 43, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 379) [M::mem_pestat] mean and std.dev: (40.03, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 500) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1052.454 CPU sec, 59.817 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666731, 54, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (92, 203, 461) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1199) [M::mem_pestat] mean and std.dev: (242.00, 251.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1568) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.54, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 50, 1087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3221) [M::mem_pestat] mean and std.dev: (379.38, 782.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4288) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1044.614 CPU sec, 46.347 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 667141, 52, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.38, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 48, 653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1923) [M::mem_pestat] mean and std.dev: (211.09, 418.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2558) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1055.667 CPU sec, 60.510 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666154, 42, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 337, 1252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3226) [M::mem_pestat] mean and std.dev: (665.45, 572.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4213) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.55, 83.00) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 32, 858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2544) [M::mem_pestat] mean and std.dev: (206.75, 439.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3387) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1050.768 CPU sec, 46.395 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 667179, 55, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 423, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1357) [M::mem_pestat] mean and std.dev: (415.31, 261.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.38, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 41, 617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1809) [M::mem_pestat] mean and std.dev: (96.95, 237.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2405) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1056.264 CPU sec, 54.916 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666794, 46, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 425, 1297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3329) [M::mem_pestat] mean and std.dev: (737.82, 732.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4345) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.45, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 24, 54) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 144) [M::mem_pestat] mean and std.dev: (21.42, 17.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 189) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1049.813 CPU sec, 58.767 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667045, 47, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 368, 726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1742) [M::mem_pestat] mean and std.dev: (501.00, 407.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2250) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.42, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 35, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (50.49, 83.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1046.709 CPU sec, 46.562 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666612, 56, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (410, 689, 2517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6731) [M::mem_pestat] mean and std.dev: (1128.00, 1242.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8838) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.54, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 32, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 193) [M::mem_pestat] mean and std.dev: (26.95, 20.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 253) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1060.213 CPU sec, 60.135 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666421, 57, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 484, 1828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5080) [M::mem_pestat] mean and std.dev: (935.78, 1149.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6706) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.67, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 32, 67) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 161) [M::mem_pestat] mean and std.dev: (31.52, 24.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 208) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1047.838 CPU sec, 46.558 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666540, 47, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.40, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 33, 75) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 195) [M::mem_pestat] mean and std.dev: (33.87, 30.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 255) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1060.227 CPU sec, 61.009 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 667250, 49, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (260, 411, 702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1586) [M::mem_pestat] mean and std.dev: (324.44, 177.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2028) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.55, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 29, 77) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 199) [M::mem_pestat] mean and std.dev: (33.27, 32.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 260) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.656 CPU sec, 45.881 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 667028, 56, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.39, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 36, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (42.71, 52.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 468) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1055.750 CPU sec, 60.808 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666923, 53, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (176, 315, 394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (250.33, 118.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1048) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.61, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 34, 328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (59.57, 94.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1264) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1044.222 CPU sec, 46.204 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666771, 41, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.62, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 35, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 170) [M::mem_pestat] mean and std.dev: (31.53, 23.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 221) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1052.652 CPU sec, 59.405 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667359, 56, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (294, 500, 2919) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8169) [M::mem_pestat] mean and std.dev: (1116.00, 1203.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10794) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.60, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 49, 617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1821) [M::mem_pestat] mean and std.dev: (118.69, 239.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2423) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1046.144 CPU sec, 46.539 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666543, 47, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.39, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 39, 1780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5308) [M::mem_pestat] mean and std.dev: (916.96, 1666.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7584) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (977, 1239, 1497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2537) [M::mem_pestat] mean and std.dev: (1099.60, 523.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3192) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1068.075 CPU sec, 59.226 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666789, 42, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (295, 438, 2128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5794) [M::mem_pestat] mean and std.dev: (1278.69, 1431.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.68, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 47, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (51.00, 53.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 693) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 493, 855) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2055) [M::mem_pestat] mean and std.dev: (503.56, 265.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2655) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1045.963 CPU sec, 57.143 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666977, 51, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 658, 830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2068) [M::mem_pestat] mean and std.dev: (572.70, 393.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2687) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.49, 82.93) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 35, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1810) [M::mem_pestat] mean and std.dev: (115.33, 264.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2407) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.867 CPU sec, 45.934 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666410, 43, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (759, 1491, 1858) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4056) [M::mem_pestat] mean and std.dev: (1313.56, 988.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5267) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.37, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 39, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 438) [M::mem_pestat] mean and std.dev: (46.44, 60.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1055.719 CPU sec, 59.914 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667074, 50, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (123, 490, 877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2385) [M::mem_pestat] mean and std.dev: (592.82, 461.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3139) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.44, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 55, 1493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4421) [M::mem_pestat] mean and std.dev: (706.34, 1311.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5951) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.814 CPU sec, 46.110 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 666405, 59, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.61, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 39, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (47.72, 60.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 617) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (437, 781, 981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2069) [M::mem_pestat] mean and std.dev: (733.20, 376.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2613) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1056.893 CPU sec, 59.622 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666872, 56, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.56, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 38, 119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 323) [M::mem_pestat] mean and std.dev: (31.64, 29.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.565 CPU sec, 46.358 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666886, 54, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.30, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 44, 381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107) [M::mem_pestat] mean and std.dev: (147.74, 275.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1470) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1061.207 CPU sec, 64.317 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667067, 50, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 472, 811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2267) [M::mem_pestat] mean and std.dev: (381.33, 293.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2995) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.63, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 48, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1681) [M::mem_pestat] mean and std.dev: (129.12, 274.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2236) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.443 CPU sec, 46.184 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 667044, 46, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.57, 83.01) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 39, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1232) [M::mem_pestat] mean and std.dev: (84.11, 170.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1638) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1053.910 CPU sec, 60.297 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 667006, 43, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.64, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 39, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1428) [M::mem_pestat] mean and std.dev: (170.97, 337.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1900) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1048.217 CPU sec, 46.641 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667322, 51, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.58, 82.96) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 40, 391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1137) [M::mem_pestat] mean and std.dev: (54.05, 82.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1510) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1057.056 CPU sec, 59.749 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666457, 45, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.33, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 31, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 228) [M::mem_pestat] mean and std.dev: (32.53, 31.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 300) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1065.365 CPU sec, 62.425 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667090, 43, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (304, 378, 813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1831) [M::mem_pestat] mean and std.dev: (515.80, 377.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2340) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.25, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 29, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (29.18, 27.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1045.665 CPU sec, 46.356 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666823, 49, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.40, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 37, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 397) [M::mem_pestat] mean and std.dev: (45.77, 50.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1053.843 CPU sec, 59.688 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666686, 52, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 320, 1158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3052) [M::mem_pestat] mean and std.dev: (466.40, 537.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3999) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.49, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 36, 1190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3526) [M::mem_pestat] mean and std.dev: (377.64, 755.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4694) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1049.357 CPU sec, 46.655 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666658, 49, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (528, 1053, 1319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2901) [M::mem_pestat] mean and std.dev: (995.09, 736.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3940) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.58, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 28, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 212) [M::mem_pestat] mean and std.dev: (33.24, 36.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 278) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1054.948 CPU sec, 61.640 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666133, 44, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.65, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 37, 612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1792) [M::mem_pestat] mean and std.dev: (151.89, 275.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2382) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1055.259 CPU sec, 47.000 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667221, 52, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 1180, 2780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7798) [M::mem_pestat] mean and std.dev: (1345.73, 1439.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10307) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.58, 82.89) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 39, 786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2330) [M::mem_pestat] mean and std.dev: (255.18, 559.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3102) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (462, 1077, 1934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4878) [M::mem_pestat] mean and std.dev: (1310.20, 979.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6350) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1050.200 CPU sec, 60.606 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667056, 52, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.54, 82.86) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 46, 1960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5848) [M::mem_pestat] mean and std.dev: (980.00, 1679.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7792) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.623 CPU sec, 46.002 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666931, 55, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.59, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 56, 832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2448) [M::mem_pestat] mean and std.dev: (337.72, 618.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3256) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1038.104 CPU sec, 59.599 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666288, 48, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (381, 516, 1537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3849) [M::mem_pestat] mean and std.dev: (881.50, 639.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5005) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.51, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 50, 97) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 235) [M::mem_pestat] mean and std.dev: (55.15, 51.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 304) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.311 CPU sec, 45.932 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666168, 50, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (278, 975, 2122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5810) [M::mem_pestat] mean and std.dev: (1143.31, 1021.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7654) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.36, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 38, 1636) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4878) [M::mem_pestat] mean and std.dev: (719.13, 1384.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6499) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1047.777 CPU sec, 60.398 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667277, 55, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (141, 283, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1419) [M::mem_pestat] mean and std.dev: (421.64, 393.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1995) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.37, 82.97) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 40, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (49.64, 62.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 556) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.787 CPU sec, 45.928 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 667253, 50, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 313, 1994) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5568) [M::mem_pestat] mean and std.dev: (1129.56, 1631.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.39, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 46, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 308) [M::mem_pestat] mean and std.dev: (38.08, 23.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1057.012 CPU sec, 60.954 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 667135, 48, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.45, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 32, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 166) [M::mem_pestat] mean and std.dev: (31.46, 21.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 215) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1046.654 CPU sec, 46.531 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666915, 55, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 557, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2873) [M::mem_pestat] mean and std.dev: (639.67, 450.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3731) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.58, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 41, 710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2090) [M::mem_pestat] mean and std.dev: (160.49, 329.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2780) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1055.488 CPU sec, 60.550 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667092, 48, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 1132, 2088) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5822) [M::mem_pestat] mean and std.dev: (948.67, 785.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7689) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.25, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 45, 2231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6661) [M::mem_pestat] mean and std.dev: (937.00, 1572.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8876) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.801 CPU sec, 46.213 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667686, 57, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (310, 499, 1254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3142) [M::mem_pestat] mean and std.dev: (754.67, 561.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4086) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.45, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 39, 1192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3544) [M::mem_pestat] mean and std.dev: (452.73, 901.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4720) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1039.089 CPU sec, 59.914 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666408, 51, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (195, 464, 1357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3681) [M::mem_pestat] mean and std.dev: (944.30, 1032.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.32, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 45, 232) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (60.85, 97.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 889) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1048.019 CPU sec, 46.754 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667205, 43, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 469, 1710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4620) [M::mem_pestat] mean and std.dev: (775.82, 752.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6075) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.60, 83.00) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 33, 203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 569) [M::mem_pestat] mean and std.dev: (66.83, 110.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 752) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1037.440 CPU sec, 58.114 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666416, 48, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 349, 494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1028) [M::mem_pestat] mean and std.dev: (310.25, 162.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.31, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 69, 1684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5014) [M::mem_pestat] mean and std.dev: (797.15, 1278.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6679) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.508 CPU sec, 46.070 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666803, 60, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.50, 82.90) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 40, 984) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2910) [M::mem_pestat] mean and std.dev: (314.26, 594.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3873) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1034.088 CPU sec, 56.422 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666507, 50, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.53, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 34, 66) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 168) [M::mem_pestat] mean and std.dev: (26.89, 18.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 219) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1035.057 CPU sec, 45.652 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666784, 57, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (290, 418, 1068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2624) [M::mem_pestat] mean and std.dev: (730.73, 648.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3402) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.34, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 29, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 552) [M::mem_pestat] mean and std.dev: (45.53, 73.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 732) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1047.363 CPU sec, 52.115 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666904, 66, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.47, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 55, 1120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3296) [M::mem_pestat] mean and std.dev: (347.23, 638.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4384) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1046.274 CPU sec, 59.395 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666655, 59, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.51, 82.96) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 46, 1115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3305) [M::mem_pestat] mean and std.dev: (465.06, 883.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4400) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.580 CPU sec, 46.072 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667302, 60, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.42, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 54, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1249) [M::mem_pestat] mean and std.dev: (157.57, 275.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1656) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1035.417 CPU sec, 58.342 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666917, 59, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 585, 1053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2505) [M::mem_pestat] mean and std.dev: (616.22, 317.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3231) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.46, 82.87) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 33, 67) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 167) [M::mem_pestat] mean and std.dev: (33.57, 31.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 217) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.623 CPU sec, 46.005 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666994, 40, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.65, 82.92) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 55, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 436) [M::mem_pestat] mean and std.dev: (59.75, 63.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 576) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1036.780 CPU sec, 58.287 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 667089, 52, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.53, 82.93) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 52, 1029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3031) [M::mem_pestat] mean and std.dev: (419.41, 806.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4032) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.901 CPU sec, 46.160 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666046, 45, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.33, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 34, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (31.40, 24.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 374) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1042.782 CPU sec, 58.373 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667377, 45, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (179, 296, 870) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2252) [M::mem_pestat] mean and std.dev: (353.12, 271.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2943) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.50, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 25, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 422) [M::mem_pestat] mean and std.dev: (35.47, 40.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 557) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1031.388 CPU sec, 45.679 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 667062, 59, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.61, 82.95) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 31, 779) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2303) [M::mem_pestat] mean and std.dev: (176.73, 378.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3065) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1039.202 CPU sec, 57.688 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666432, 40, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.38, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 41, 609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1791) [M::mem_pestat] mean and std.dev: (196.09, 327.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2382) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.717 CPU sec, 46.126 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 667118, 56, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.41, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 56, 957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2821) [M::mem_pestat] mean and std.dev: (333.22, 598.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3753) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (396, 1403, 2424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6480) [M::mem_pestat] mean and std.dev: (1399.78, 992.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8508) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1048.579 CPU sec, 56.152 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666621, 42, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (468, 964, 1467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3465) [M::mem_pestat] mean and std.dev: (1015.91, 769.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.27, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 38, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1297) [M::mem_pestat] mean and std.dev: (128.23, 238.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1723) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1050.179 CPU sec, 59.266 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 666559, 41, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.38, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 32, 1249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3717) [M::mem_pestat] mean and std.dev: (367.65, 743.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4951) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.979 CPU sec, 45.923 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666830, 41, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 696, 1293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3475) [M::mem_pestat] mean and std.dev: (684.31, 547.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4566) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.35, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 34, 1867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5567) [M::mem_pestat] mean and std.dev: (853.85, 1514.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7417) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1044.309 CPU sec, 60.121 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667229, 58, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (93, 265, 372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 930) [M::mem_pestat] mean and std.dev: (248.27, 231.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1209) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.31, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 48, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1244) [M::mem_pestat] mean and std.dev: (84.09, 192.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1653) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.600 CPU sec, 45.978 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666453, 39, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.52, 82.90) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 35, 1001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2963) [M::mem_pestat] mean and std.dev: (331.63, 674.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3944) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1040.249 CPU sec, 57.929 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667081, 52, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 784, 1393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3725) [M::mem_pestat] mean and std.dev: (712.09, 549.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4891) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.35, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 50, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (47.00, 60.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.265 CPU sec, 46.005 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666607, 62, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.48, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 41, 409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1201) [M::mem_pestat] mean and std.dev: (71.06, 179.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1597) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1035.241 CPU sec, 57.741 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666901, 53, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.35, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 32, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (37.33, 38.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.524 CPU sec, 45.979 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 667364, 44, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.67, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 40, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (65.28, 98.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1273) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.887 CPU sec, 57.913 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666998, 57, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.65, 82.85) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 54, 1008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2968) [M::mem_pestat] mean and std.dev: (335.55, 686.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3948) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.823 CPU sec, 46.143 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666714, 45, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.61, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 53, 1726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5134) [M::mem_pestat] mean and std.dev: (749.86, 1376.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6838) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1049.055 CPU sec, 59.854 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667034, 43, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (773, 1018, 2628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6338) [M::mem_pestat] mean and std.dev: (1163.36, 1058.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8193) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.45, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 33, 71) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 181) [M::mem_pestat] mean and std.dev: (34.27, 29.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 236) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1046.240 CPU sec, 60.909 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666955, 47, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 462, 923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2275) [M::mem_pestat] mean and std.dev: (548.18, 397.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2951) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.37, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 29, 77) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 205) [M::mem_pestat] mean and std.dev: (31.42, 31.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 269) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.692 CPU sec, 46.207 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667073, 47, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 561, 1318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3424) [M::mem_pestat] mean and std.dev: (756.36, 622.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.39, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 38, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1273) [M::mem_pestat] mean and std.dev: (171.72, 308.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1691) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1040.091 CPU sec, 58.077 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666854, 50, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.46, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 47, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (45.52, 45.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.044 CPU sec, 46.165 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666568, 46, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 439, 702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1806) [M::mem_pestat] mean and std.dev: (339.60, 206.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.57, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 55, 1492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4424) [M::mem_pestat] mean and std.dev: (776.05, 1241.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5890) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1043.594 CPU sec, 58.557 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666876, 53, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (286, 371, 1680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4468) [M::mem_pestat] mean and std.dev: (958.20, 929.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.48, 83.01) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 33, 758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2242) [M::mem_pestat] mean and std.dev: (225.76, 486.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2984) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.829 CPU sec, 46.233 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666467, 52, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 287, 665) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1567) [M::mem_pestat] mean and std.dev: (346.50, 274.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2018) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.57, 82.87) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 37, 273) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 771) [M::mem_pestat] mean and std.dev: (67.16, 97.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1020) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1045.201 CPU sec, 58.373 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 666583, 54, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.42, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 37, 785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2319) [M::mem_pestat] mean and std.dev: (78.00, 173.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3086) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1031.702 CPU sec, 45.769 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667011, 48, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 754, 1483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3959) [M::mem_pestat] mean and std.dev: (825.80, 664.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5197) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.38, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 44, 1256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3718) [M::mem_pestat] mean and std.dev: (432.16, 806.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4949) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1035.177 CPU sec, 57.819 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666414, 43, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.30, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 45, 1750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5210) [M::mem_pestat] mean and std.dev: (698.13, 1310.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6940) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.403 CPU sec, 46.173 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 667030, 54, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.45, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 28, 75) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 195) [M::mem_pestat] mean and std.dev: (29.77, 24.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 255) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1033.849 CPU sec, 58.789 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667297, 38, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.47, 82.89) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 29, 254) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 730) [M::mem_pestat] mean and std.dev: (32.86, 44.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1055.686 CPU sec, 61.471 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 666757, 45, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.34, 82.89) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 40, 741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2193) [M::mem_pestat] mean and std.dev: (260.83, 527.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2919) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1052.081 CPU sec, 46.568 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667391, 44, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (247, 302, 442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 832) [M::mem_pestat] mean and std.dev: (258.25, 108.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.49, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 68, 2451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7307) [M::mem_pestat] mean and std.dev: (1028.21, 1660.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9735) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1059.649 CPU sec, 61.732 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666498, 50, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (151, 267, 607) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1519) [M::mem_pestat] mean and std.dev: (338.64, 310.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1975) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.57, 82.95) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 53, 1242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3684) [M::mem_pestat] mean and std.dev: (514.69, 932.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4905) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1056.284 CPU sec, 46.868 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666742, 33, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.48, 83.12) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 52, 1774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5266) [M::mem_pestat] mean and std.dev: (500.27, 1023.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7012) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1061.019 CPU sec, 61.504 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666838, 53, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.30, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 40, 116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 304) [M::mem_pestat] mean and std.dev: (32.66, 22.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 398) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1047.989 CPU sec, 46.547 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666439, 39, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.45, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 33, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (45.61, 54.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 677) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1057.270 CPU sec, 60.395 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666612, 59, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 443, 982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2504) [M::mem_pestat] mean and std.dev: (618.43, 551.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3265) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.66, 82.88) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 45, 180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 488) [M::mem_pestat] mean and std.dev: (55.11, 72.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 642) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1056.900 CPU sec, 46.876 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666135, 43, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.43, 82.91) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 27, 439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1279) [M::mem_pestat] mean and std.dev: (104.83, 220.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1699) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (786, 1216, 2564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6120) [M::mem_pestat] mean and std.dev: (1634.60, 1597.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8026) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1063.380 CPU sec, 61.494 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666315, 66, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.60, 82.99) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 38, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (38.10, 37.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 572) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1060.941 CPU sec, 46.960 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666752, 61, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (298, 495, 1741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4627) [M::mem_pestat] mean and std.dev: (685.90, 654.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6070) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.45, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 65, 2582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7708) [M::mem_pestat] mean and std.dev: (1109.93, 1791.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10271) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1052.824 CPU sec, 59.450 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666196, 50, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 358, 427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 829) [M::mem_pestat] mean and std.dev: (342.31, 155.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1030) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.52, 82.92) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 47, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 706) [M::mem_pestat] mean and std.dev: (67.58, 113.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 935) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (261, 1241, 1547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4119) [M::mem_pestat] mean and std.dev: (1028.00, 667.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5405) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1061.034 CPU sec, 46.935 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666845, 52, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.39, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 42, 980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2898) [M::mem_pestat] mean and std.dev: (413.64, 847.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3857) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1063.616 CPU sec, 61.371 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667113, 37, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (354, 568, 943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2121) [M::mem_pestat] mean and std.dev: (583.30, 403.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.36, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 46, 1180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3488) [M::mem_pestat] mean and std.dev: (151.83, 310.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4642) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1052.819 CPU sec, 46.538 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666888, 55, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.51, 82.99) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 39, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (45.33, 49.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1063.958 CPU sec, 60.878 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 667263, 40, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.29, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 43, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1234) [M::mem_pestat] mean and std.dev: (79.16, 178.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1639) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (703, 957, 1063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1783) [M::mem_pestat] mean and std.dev: (827.00, 269.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2143) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1052.221 CPU sec, 46.507 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 666695, 63, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.30, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 32, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 239) [M::mem_pestat] mean and std.dev: (31.86, 29.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 313) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1054.691 CPU sec, 60.357 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666926, 52, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.45, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 43, 851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2515) [M::mem_pestat] mean and std.dev: (313.59, 636.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3347) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1054.390 CPU sec, 46.840 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666386, 47, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.61, 82.95) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 32, 1120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3326) [M::mem_pestat] mean and std.dev: (314.68, 628.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4429) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1060.000 CPU sec, 60.877 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666985, 48, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.43, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 45, 1198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3546) [M::mem_pestat] mean and std.dev: (388.98, 779.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4720) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1051.818 CPU sec, 46.596 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666728, 59, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (346, 474, 2251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6061) [M::mem_pestat] mean and std.dev: (850.25, 731.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7966) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.23, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 41, 437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1285) [M::mem_pestat] mean and std.dev: (101.15, 241.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1709) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1064.267 CPU sec, 61.030 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667512, 35, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.37, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 52, 1163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3443) [M::mem_pestat] mean and std.dev: (599.06, 1027.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4711) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1053.938 CPU sec, 46.684 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666830, 49, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.55, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 36, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 482) [M::mem_pestat] mean and std.dev: (44.79, 50.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 638) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1052.916 CPU sec, 55.969 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667026, 48, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (241, 933, 1069) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2725) [M::mem_pestat] mean and std.dev: (773.60, 543.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.50, 82.89) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 40, 424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1232) [M::mem_pestat] mean and std.dev: (137.95, 266.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1636) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1060.255 CPU sec, 62.081 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666788, 49, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 324, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1133) [M::mem_pestat] mean and std.dev: (326.75, 196.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1445) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.61, 83.02) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 37, 741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2205) [M::mem_pestat] mean and std.dev: (258.82, 529.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2937) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1049.788 CPU sec, 46.597 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667458, 46, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 442, 982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2384) [M::mem_pestat] mean and std.dev: (529.40, 366.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3085) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.63, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 55, 1126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3326) [M::mem_pestat] mean and std.dev: (398.20, 772.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4426) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1054.724 CPU sec, 60.651 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666804, 52, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.34, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 56, 1078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3200) [M::mem_pestat] mean and std.dev: (250.64, 541.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4261) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1056.081 CPU sec, 46.641 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666364, 45, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.37, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 34, 770) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2282) [M::mem_pestat] mean and std.dev: (198.74, 473.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3038) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (281, 993, 1251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3191) [M::mem_pestat] mean and std.dev: (821.78, 449.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4161) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1061.314 CPU sec, 61.188 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 666379, 55, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.54, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 33, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 363) [M::mem_pestat] mean and std.dev: (29.40, 31.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1055.933 CPU sec, 46.755 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666815, 40, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.32, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 43, 91) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 241) [M::mem_pestat] mean and std.dev: (33.29, 23.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 316) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1055.728 CPU sec, 61.221 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666390, 51, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (309, 1017, 4226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12060) [M::mem_pestat] mean and std.dev: (2115.00, 2429.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15977) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.40, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 66, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (64.54, 59.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 851) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1060.066 CPU sec, 47.021 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 666511, 58, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (293, 557, 1657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4385) [M::mem_pestat] mean and std.dev: (684.19, 608.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.49, 82.86) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 33, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 876) [M::mem_pestat] mean and std.dev: (51.02, 83.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1162) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1064.922 CPU sec, 61.995 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666821, 54, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 766, 1473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3769) [M::mem_pestat] mean and std.dev: (773.70, 536.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.62, 82.85) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 48, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (54.93, 68.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (859, 1399, 3133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7681) [M::mem_pestat] mean and std.dev: (1923.55, 1856.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9955) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1054.362 CPU sec, 46.685 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666564, 37, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.41, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 45, 2856) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8528) [M::mem_pestat] mean and std.dev: (859.49, 1420.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11364) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (659, 1698, 1952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4538) [M::mem_pestat] mean and std.dev: (1393.22, 794.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5831) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1059.062 CPU sec, 58.145 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666853, 54, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (355, 867, 1805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4705) [M::mem_pestat] mean and std.dev: (907.83, 680.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6155) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.48, 82.86) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 60, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1546) [M::mem_pestat] mean and std.dev: (206.43, 380.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2055) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1054.687 CPU sec, 61.084 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667412, 60, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (266, 456, 1616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4316) [M::mem_pestat] mean and std.dev: (656.91, 562.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5666) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.49, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 39, 277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (59.30, 82.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1057) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1059.602 CPU sec, 47.051 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666590, 61, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.49, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 29, 436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1284) [M::mem_pestat] mean and std.dev: (123.83, 236.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1708) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (905, 1800, 3650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9140) [M::mem_pestat] mean and std.dev: (2718.70, 2421.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12403) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1058.425 CPU sec, 61.305 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666382, 46, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (127, 286, 539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1363) [M::mem_pestat] mean and std.dev: (310.11, 253.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.42, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 45, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (65.92, 86.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 621) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1060.965 CPU sec, 47.186 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666199, 57, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.32, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 42, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986) [M::mem_pestat] mean and std.dev: (54.02, 75.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1306) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1066.475 CPU sec, 61.733 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666772, 43, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.55, 82.94) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 37, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1268) [M::mem_pestat] mean and std.dev: (158.55, 316.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1685) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1057.128 CPU sec, 46.937 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666312, 75, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.56, 83.01) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 32, 120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (45.51, 59.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1061.563 CPU sec, 61.840 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667520, 51, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (150, 322, 570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1410) [M::mem_pestat] mean and std.dev: (421.93, 299.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1830) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.56, 82.97) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 35, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 331) [M::mem_pestat] mean and std.dev: (39.93, 45.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (902, 1448, 2935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7001) [M::mem_pestat] mean and std.dev: (1840.70, 1243.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9034) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1052.070 CPU sec, 46.451 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667207, 51, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 262, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045) [M::mem_pestat] mean and std.dev: (218.00, 136.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1352) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.64, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 30, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 631) [M::mem_pestat] mean and std.dev: (42.88, 61.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 836) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1062.633 CPU sec, 62.055 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666891, 48, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (245, 348, 926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2288) [M::mem_pestat] mean and std.dev: (554.31, 551.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.67, 82.97) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 32, 167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (43.77, 52.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 623) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1054.983 CPU sec, 46.752 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666716, 48, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.62, 82.93) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 35, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1324) [M::mem_pestat] mean and std.dev: (82.84, 142.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1760) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1066.347 CPU sec, 61.850 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666712, 40, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (96, 358, 842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2334) [M::mem_pestat] mean and std.dev: (534.64, 549.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3080) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.49, 82.86) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 52, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 432) [M::mem_pestat] mean and std.dev: (57.76, 56.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 569) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1065.116 CPU sec, 61.715 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666263, 61, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (342, 463, 1322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3282) [M::mem_pestat] mean and std.dev: (577.10, 493.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4262) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.36, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 35, 65) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 161) [M::mem_pestat] mean and std.dev: (32.59, 27.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 209) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1060.691 CPU sec, 46.983 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666670, 53, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 268, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 585) [M::mem_pestat] mean and std.dev: (210.10, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 717) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.43, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 42, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 858) [M::mem_pestat] mean and std.dev: (51.10, 66.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1138) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1063.467 CPU sec, 60.533 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666343, 48, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (157, 266, 352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 742) [M::mem_pestat] mean and std.dev: (242.50, 122.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 937) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.46, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 35, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (44.70, 47.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 453) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1060.569 CPU sec, 46.910 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 667417, 63, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.38, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 41, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (50.37, 63.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 454) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1056.252 CPU sec, 60.262 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 666946, 51, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.42, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 29, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 252) [M::mem_pestat] mean and std.dev: (40.33, 46.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 331) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1056.088 CPU sec, 46.824 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666177, 46, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.45, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 42, 2027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6057) [M::mem_pestat] mean and std.dev: (1010.78, 1554.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8072) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1063.431 CPU sec, 62.292 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666588, 41, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.35, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 39, 1136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3368) [M::mem_pestat] mean and std.dev: (381.14, 793.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4484) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1062.309 CPU sec, 47.006 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666643, 44, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.56, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 51, 1201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3549) [M::mem_pestat] mean and std.dev: (224.75, 521.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4723) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1061.676 CPU sec, 60.945 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 667109, 52, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.48, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 53, 990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2918) [M::mem_pestat] mean and std.dev: (277.82, 614.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3882) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1057.019 CPU sec, 46.801 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667014, 51, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.73, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 45, 1296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3828) [M::mem_pestat] mean and std.dev: (421.56, 796.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5094) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1065.386 CPU sec, 60.437 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666811, 48, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 417, 3982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11384) [M::mem_pestat] mean and std.dev: (1716.27, 2162.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15085) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.56, 82.98) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 38, 809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2391) [M::mem_pestat] mean and std.dev: (223.95, 554.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3182) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1058.621 CPU sec, 46.972 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667064, 62, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (116, 320, 1838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5282) [M::mem_pestat] mean and std.dev: (906.00, 925.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.37, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 35, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (41.40, 44.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1059.536 CPU sec, 61.418 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666342, 45, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 290, 1465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4083) [M::mem_pestat] mean and std.dev: (854.73, 990.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5392) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.61, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 39, 103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259) [M::mem_pestat] mean and std.dev: (40.00, 30.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1053.355 CPU sec, 46.634 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 667150, 53, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.46, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 40, 657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1937) [M::mem_pestat] mean and std.dev: (136.28, 343.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2577) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1055.112 CPU sec, 60.659 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666935, 48, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 415, 1169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3097) [M::mem_pestat] mean and std.dev: (623.92, 570.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.37, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 45, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 465) [M::mem_pestat] mean and std.dev: (51.87, 59.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1052.273 CPU sec, 46.756 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666590, 34, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.57, 82.94) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 26, 61) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 155) [M::mem_pestat] mean and std.dev: (26.56, 23.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 202) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1061.002 CPU sec, 60.789 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666976, 54, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.56, 82.96) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 46, 615) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1817) [M::mem_pestat] mean and std.dev: (184.86, 386.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2418) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (292, 624, 1327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3397) [M::mem_pestat] mean and std.dev: (920.27, 770.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4432) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1059.314 CPU sec, 46.992 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666446, 39, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (280, 349, 707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1561) [M::mem_pestat] mean and std.dev: (417.67, 249.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1988) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.40, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 35, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 231) [M::mem_pestat] mean and std.dev: (32.61, 24.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 300) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1064.720 CPU sec, 61.554 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666790, 57, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (267, 865, 1134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2868) [M::mem_pestat] mean and std.dev: (733.69, 591.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.60, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 40, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (55.89, 94.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 870) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (300, 695, 1181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2943) [M::mem_pestat] mean and std.dev: (669.89, 442.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3824) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1052.611 CPU sec, 46.637 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666768, 52, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 338, 1292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3446) [M::mem_pestat] mean and std.dev: (823.69, 964.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.49, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 44, 1314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3906) [M::mem_pestat] mean and std.dev: (596.47, 1132.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5202) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1064.617 CPU sec, 61.102 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 667149, 36, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.48, 82.95) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 32, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 943) [M::mem_pestat] mean and std.dev: (57.21, 101.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1252) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1046.922 CPU sec, 46.164 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 667111, 45, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.40, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 50, 1339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3977) [M::mem_pestat] mean and std.dev: (457.66, 873.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5296) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1050.445 CPU sec, 54.454 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 665986, 46, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.23, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (8, 32, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 380) [M::mem_pestat] mean and std.dev: (37.83, 62.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 504) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1067.585 CPU sec, 60.945 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 666985, 52, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.43, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 49, 1640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4872) [M::mem_pestat] mean and std.dev: (616.38, 1159.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6488) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1060.309 CPU sec, 46.849 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667353, 51, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (293, 533, 1216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3062) [M::mem_pestat] mean and std.dev: (665.60, 499.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3985) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.52, 83.01) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 34, 187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (60.34, 96.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 694) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (762, 1049, 1267) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2277) [M::mem_pestat] mean and std.dev: (942.00, 525.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3044) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1066.809 CPU sec, 61.947 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 667222, 55, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.44, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 25, 77) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 201) [M::mem_pestat] mean and std.dev: (22.05, 15.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 263) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1052.160 CPU sec, 46.688 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666674, 51, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.60, 83.05) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 33, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 430) [M::mem_pestat] mean and std.dev: (51.64, 76.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 568) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1062.798 CPU sec, 61.344 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 667302, 39, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 368, 811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2011) [M::mem_pestat] mean and std.dev: (458.88, 418.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2611) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.46, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 34, 271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 773) [M::mem_pestat] mean and std.dev: (43.70, 58.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1024) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1052.958 CPU sec, 46.609 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667174, 46, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (220, 406, 1012) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2596) [M::mem_pestat] mean and std.dev: (520.67, 412.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3388) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.65, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 36, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 223) [M::mem_pestat] mean and std.dev: (32.24, 29.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 293) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1057.975 CPU sec, 61.107 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666970, 55, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (332, 462, 789) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1703) [M::mem_pestat] mean and std.dev: (500.40, 398.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2160) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.41, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 49, 917) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2695) [M::mem_pestat] mean and std.dev: (366.15, 669.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3584) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1050.988 CPU sec, 46.572 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 667239, 37, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.50, 82.86) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 57, 955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2819) [M::mem_pestat] mean and std.dev: (202.71, 408.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3751) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1060.099 CPU sec, 61.709 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666607, 50, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.52, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 34, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (45.46, 71.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1180) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1059.851 CPU sec, 46.890 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666664, 47, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.46, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 52, 1895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5657) [M::mem_pestat] mean and std.dev: (976.56, 1651.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7583) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1056.233 CPU sec, 58.354 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666398, 37, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (193, 330, 1638) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4528) [M::mem_pestat] mean and std.dev: (632.78, 705.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5973) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.51, 82.87) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 20, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1834) [M::mem_pestat] mean and std.dev: (103.81, 255.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2442) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1067.076 CPU sec, 61.995 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666602, 56, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (166, 304, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1114) [M::mem_pestat] mean and std.dev: (246.40, 134.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1430) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.32, 82.86) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 45, 995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2943) [M::mem_pestat] mean and std.dev: (294.17, 611.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3917) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1057.940 CPU sec, 46.732 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666819, 57, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.40, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 37, 419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1223) [M::mem_pestat] mean and std.dev: (131.33, 235.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1625) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1062.807 CPU sec, 60.839 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666814, 55, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (207, 909, 1481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4029) [M::mem_pestat] mean and std.dev: (910.70, 751.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5303) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.46, 82.90) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 40, 128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (44.34, 49.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1059.699 CPU sec, 46.966 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666957, 51, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (55, 293, 6010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 17920) [M::mem_pestat] mean and std.dev: (2389.09, 3093.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 23875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.51, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 45, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 595) [M::mem_pestat] mean and std.dev: (76.95, 121.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 787) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1054.089 CPU sec, 60.845 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 666691, 44, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.45, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 41, 1455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4333) [M::mem_pestat] mean and std.dev: (335.32, 709.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5772) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1056.510 CPU sec, 46.837 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666830, 70, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (372, 523, 801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1659) [M::mem_pestat] mean and std.dev: (529.56, 240.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2088) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.41, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 28, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 195) [M::mem_pestat] mean and std.dev: (32.22, 31.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 256) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1063.351 CPU sec, 61.169 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666728, 37, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 495, 805) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1817) [M::mem_pestat] mean and std.dev: (536.15, 409.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2323) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.33, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 42, 1226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3648) [M::mem_pestat] mean and std.dev: (355.58, 739.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4859) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (203, 977, 1603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4403) [M::mem_pestat] mean and std.dev: (1401.20, 1506.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7427) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1060.251 CPU sec, 47.035 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 667381, 45, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.54, 82.92) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 38, 501) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1475) [M::mem_pestat] mean and std.dev: (128.26, 242.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1962) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1056.988 CPU sec, 60.045 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666738, 50, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (124, 217, 2450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7102) [M::mem_pestat] mean and std.dev: (1131.80, 1744.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.60, 82.97) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 27, 55) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 137) [M::mem_pestat] mean and std.dev: (33.27, 30.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 178) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1052.396 CPU sec, 46.699 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667075, 57, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (168, 282, 1129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3051) [M::mem_pestat] mean and std.dev: (695.33, 792.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4012) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.65, 83.00) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 46, 2077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6193) [M::mem_pestat] mean and std.dev: (628.65, 1201.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8251) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1060.476 CPU sec, 59.906 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666523, 41, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.45, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 39, 977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2891) [M::mem_pestat] mean and std.dev: (434.05, 762.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3848) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1065.383 CPU sec, 60.930 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 667025, 43, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.60, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 47, 640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1882) [M::mem_pestat] mean and std.dev: (172.89, 307.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2503) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1057.218 CPU sec, 46.888 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666300, 46, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.49, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 36, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 639) [M::mem_pestat] mean and std.dev: (57.89, 86.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 846) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1059.830 CPU sec, 61.331 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666677, 38, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 327, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1074) [M::mem_pestat] mean and std.dev: (324.91, 248.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1372) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.34, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 33, 417) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1215) [M::mem_pestat] mean and std.dev: (109.62, 215.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1614) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1058.505 CPU sec, 46.951 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666353, 69, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.48, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 54, 333) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 953) [M::mem_pestat] mean and std.dev: (72.02, 121.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1263) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1065.324 CPU sec, 61.593 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666739, 46, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.53, 82.95) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 34, 1005) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2977) [M::mem_pestat] mean and std.dev: (215.53, 547.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3963) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1048.923 CPU sec, 46.200 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667269, 52, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (225, 715, 1339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3567) [M::mem_pestat] mean and std.dev: (876.54, 915.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.32, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 44, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (42.05, 37.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1061.622 CPU sec, 60.856 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666610, 42, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.47, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 48, 1702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5080) [M::mem_pestat] mean and std.dev: (721.92, 1351.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6769) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1058.755 CPU sec, 46.763 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666756, 39, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.37, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 45, 2655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7933) [M::mem_pestat] mean and std.dev: (810.70, 1313.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10572) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1063.071 CPU sec, 60.722 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 667411, 39, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.49, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 30, 1045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3093) [M::mem_pestat] mean and std.dev: (214.38, 555.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4117) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1055.747 CPU sec, 46.825 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666812, 46, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.47, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 49, 1268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3772) [M::mem_pestat] mean and std.dev: (447.20, 1009.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5024) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1066.536 CPU sec, 61.403 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666927, 43, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.47, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 67, 1292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3806) [M::mem_pestat] mean and std.dev: (276.65, 612.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5063) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1055.988 CPU sec, 46.641 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666425, 46, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.53, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 26, 54) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 142) [M::mem_pestat] mean and std.dev: (22.61, 17.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 186) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1065.202 CPU sec, 59.839 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666631, 50, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (254, 325, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 842) [M::mem_pestat] mean and std.dev: (303.33, 99.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1038) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.40, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 33, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (46.27, 58.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1056.228 CPU sec, 46.683 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 666508, 51, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.52, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 59, 1214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3596) [M::mem_pestat] mean and std.dev: (334.42, 636.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4787) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1064.734 CPU sec, 61.318 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666707, 42, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.66, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 47, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (46.46, 45.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 405) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1060.863 CPU sec, 46.998 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667255, 43, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (267, 627, 1085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2721) [M::mem_pestat] mean and std.dev: (654.27, 624.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3539) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.50, 82.95) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 32, 488) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1440) [M::mem_pestat] mean and std.dev: (71.24, 192.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1916) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1055.095 CPU sec, 59.820 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666707, 44, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.29, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (62.14, 96.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1056.369 CPU sec, 46.834 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666716, 50, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (313, 943, 2385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6529) [M::mem_pestat] mean and std.dev: (1509.00, 1567.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.38, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 40, 75) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 193) [M::mem_pestat] mean and std.dev: (34.38, 28.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 252) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1058.510 CPU sec, 61.084 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 667264, 54, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.40, 82.90) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 33, 163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 461) [M::mem_pestat] mean and std.dev: (45.36, 51.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 610) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1058.118 CPU sec, 47.006 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 667140, 40, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (169, 342, 1178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3196) [M::mem_pestat] mean and std.dev: (489.00, 703.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4205) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.36, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 44, 874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2604) [M::mem_pestat] mean and std.dev: (174.12, 435.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3469) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1058.460 CPU sec, 60.123 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666314, 55, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.48, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 37, 820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2428) [M::mem_pestat] mean and std.dev: (246.81, 558.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3232) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1054.823 CPU sec, 46.792 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666852, 34, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (175, 1047, 1511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4183) [M::mem_pestat] mean and std.dev: (638.62, 581.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.43, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 58, 571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1637) [M::mem_pestat] mean and std.dev: (188.93, 330.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2170) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1044.884 CPU sec, 51.864 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666542, 48, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (133, 346, 506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1252) [M::mem_pestat] mean and std.dev: (325.58, 285.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1625) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.33, 82.85) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 34, 661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1953) [M::mem_pestat] mean and std.dev: (156.36, 384.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2599) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1058.295 CPU sec, 60.131 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667064, 53, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (108, 237, 1748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5028) [M::mem_pestat] mean and std.dev: (550.10, 677.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.46, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 42, 158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 440) [M::mem_pestat] mean and std.dev: (46.56, 50.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 581) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1049.338 CPU sec, 46.347 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666734, 49, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.41, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 33, 67) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 163) [M::mem_pestat] mean and std.dev: (33.12, 26.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 211) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1056.140 CPU sec, 59.678 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666596, 45, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 1020, 1649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4401) [M::mem_pestat] mean and std.dev: (843.33, 670.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5777) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.32, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 25, 85) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 233) [M::mem_pestat] mean and std.dev: (34.41, 39.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 307) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1055.905 CPU sec, 46.720 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666327, 53, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (298, 531, 1526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3982) [M::mem_pestat] mean and std.dev: (810.40, 634.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5210) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.47, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 46, 820) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2422) [M::mem_pestat] mean and std.dev: (282.37, 542.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3223) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1055.841 CPU sec, 60.756 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666354, 45, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.38, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 40, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (39.14, 32.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1055.296 CPU sec, 46.763 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666578, 45, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 617, 1481) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3941) [M::mem_pestat] mean and std.dev: (566.62, 478.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5171) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.38, 82.89) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 47, 773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2267) [M::mem_pestat] mean and std.dev: (250.51, 504.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3014) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1059.358 CPU sec, 61.208 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 667185, 43, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.75, 82.96) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 41, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (57.29, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 798) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1049.293 CPU sec, 46.486 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667096, 51, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (293, 487, 1521) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3977) [M::mem_pestat] mean and std.dev: (830.70, 688.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5205) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.65, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 31, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1229) [M::mem_pestat] mean and std.dev: (121.91, 273.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1633) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1053.286 CPU sec, 61.023 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666448, 53, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.37, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 45, 1694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5050) [M::mem_pestat] mean and std.dev: (785.57, 1336.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6728) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1052.282 CPU sec, 46.539 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666919, 62, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (332, 439, 1306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3254) [M::mem_pestat] mean and std.dev: (846.00, 799.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4228) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.45, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 45, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1748) [M::mem_pestat] mean and std.dev: (131.47, 297.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2323) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1047.089 CPU sec, 56.380 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666882, 39, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.52, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 37, 78) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 188) [M::mem_pestat] mean and std.dev: (43.91, 37.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 243) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1043.371 CPU sec, 59.206 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666838, 42, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.72, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 38, 124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (40.56, 40.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 448) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (591, 797, 1505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3333) [M::mem_pestat] mean and std.dev: (853.00, 488.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4247) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1043.235 CPU sec, 46.148 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666521, 56, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (180, 217, 828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2124) [M::mem_pestat] mean and std.dev: (467.08, 501.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.40, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 31, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (41.96, 54.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (229, 1520, 3055) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8707) [M::mem_pestat] mean and std.dev: (1771.10, 1844.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11533) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1044.972 CPU sec, 59.731 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667006, 43, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1097, 1523, 1740) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3026) [M::mem_pestat] mean and std.dev: (1386.40, 521.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.65, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 24, 159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 453) [M::mem_pestat] mean and std.dev: (44.11, 80.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 600) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.324 CPU sec, 46.217 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 666396, 60, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.30, 82.91) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 47, 553) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1627) [M::mem_pestat] mean and std.dev: (178.69, 377.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2164) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1046.168 CPU sec, 58.813 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 666873, 61, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 356, 1078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2792) [M::mem_pestat] mean and std.dev: (482.28, 436.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.52, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 36, 829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2457) [M::mem_pestat] mean and std.dev: (265.94, 571.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3271) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (788, 1049, 1502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2930) [M::mem_pestat] mean and std.dev: (932.88, 313.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3644) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1045.163 CPU sec, 46.250 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666576, 40, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (284, 554, 1008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2456) [M::mem_pestat] mean and std.dev: (765.88, 716.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3632) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.49, 82.85) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 51, 792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2342) [M::mem_pestat] mean and std.dev: (241.77, 516.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3117) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1049.890 CPU sec, 60.217 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666860, 51, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.39, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 50, 641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1877) [M::mem_pestat] mean and std.dev: (229.34, 451.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2495) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.192 CPU sec, 46.116 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666915, 49, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (121, 309, 1587) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4519) [M::mem_pestat] mean and std.dev: (1031.86, 1289.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6190) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.61, 82.90) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 41, 1018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3022) [M::mem_pestat] mean and std.dev: (411.36, 855.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4024) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1042.350 CPU sec, 58.642 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666799, 47, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (177, 330, 681) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1689) [M::mem_pestat] mean and std.dev: (422.70, 314.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2193) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.38, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 45, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 657) [M::mem_pestat] mean and std.dev: (54.08, 66.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 867) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.100 CPU sec, 46.255 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 667139, 40, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (259, 414, 974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2404) [M::mem_pestat] mean and std.dev: (709.70, 602.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3122) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.44, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (7, 40, 577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1717) [M::mem_pestat] mean and std.dev: (118.74, 222.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2287) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1044.017 CPU sec, 59.437 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 667080, 33, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.48, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 34, 82) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 222) [M::mem_pestat] mean and std.dev: (32.93, 29.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 292) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.816 CPU sec, 56.777 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666831, 49, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.51, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 40, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 745) [M::mem_pestat] mean and std.dev: (43.53, 50.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.914 CPU sec, 46.186 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666281, 57, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.53, 82.93) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 33, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062) [M::mem_pestat] mean and std.dev: (44.45, 64.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1412) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.717 CPU sec, 57.775 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 665923, 47, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (272, 1255, 5146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14894) [M::mem_pestat] mean and std.dev: (2020.08, 2385.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.57, 83.00) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 42, 179) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 481) [M::mem_pestat] mean and std.dev: (46.84, 61.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 632) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.885 CPU sec, 46.050 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666389, 28, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.45, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 32, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 173) [M::mem_pestat] mean and std.dev: (31.12, 26.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 225) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1039.691 CPU sec, 57.958 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666174, 51, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.48, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 45, 272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 768) [M::mem_pestat] mean and std.dev: (88.29, 171.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1016) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.414 CPU sec, 46.221 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 667282, 54, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 286, 739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1805) [M::mem_pestat] mean and std.dev: (548.57, 517.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2617) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.45, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 38, 826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2438) [M::mem_pestat] mean and std.dev: (182.56, 397.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3244) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1040.869 CPU sec, 57.558 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 667568, 55, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.33, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 42, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (47.48, 58.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1034.797 CPU sec, 45.769 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666302, 52, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.73, 82.86) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 50, 4161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12445) [M::mem_pestat] mean and std.dev: (1663.87, 2522.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16587) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1041.275 CPU sec, 58.612 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666604, 52, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (288, 710, 1753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4683) [M::mem_pestat] mean and std.dev: (703.64, 587.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6148) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.41, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 57, 1980) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5896) [M::mem_pestat] mean and std.dev: (812.40, 1294.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7854) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.933 CPU sec, 46.055 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 666938, 46, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.57, 83.00) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 41, 134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 372) [M::mem_pestat] mean and std.dev: (64.25, 79.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.704 CPU sec, 58.964 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666760, 51, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.51, 82.85) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 79, 2424) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7210) [M::mem_pestat] mean and std.dev: (919.17, 1455.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9603) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1039.728 CPU sec, 57.573 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666421, 46, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (58, 257, 337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 895) [M::mem_pestat] mean and std.dev: (196.89, 145.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.38, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 58, 955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2825) [M::mem_pestat] mean and std.dev: (255.18, 525.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3760) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (563, 670, 1308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2798) [M::mem_pestat] mean and std.dev: (763.44, 390.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3543) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1040.345 CPU sec, 46.059 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 667411, 54, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.59, 82.87) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 38, 995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2957) [M::mem_pestat] mean and std.dev: (301.08, 608.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3938) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1033.100 CPU sec, 57.842 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666642, 55, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.31, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 35, 763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2263) [M::mem_pestat] mean and std.dev: (197.55, 467.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3013) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.657 CPU sec, 45.890 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667225, 55, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (73, 253, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 766) [M::mem_pestat] mean and std.dev: (205.89, 157.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 997) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.51, 82.86) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 37, 1243) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3701) [M::mem_pestat] mean and std.dev: (338.40, 704.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4930) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1034.673 CPU sec, 57.698 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 667345, 42, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 361, 1637) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4287) [M::mem_pestat] mean and std.dev: (771.57, 790.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5612) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.57, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 63, 1729) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5125) [M::mem_pestat] mean and std.dev: (771.05, 1298.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6823) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1032.722 CPU sec, 45.698 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666810, 49, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (303, 447, 591) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1167) [M::mem_pestat] mean and std.dev: (405.50, 206.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.37, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 29, 735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2175) [M::mem_pestat] mean and std.dev: (175.19, 381.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2895) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1037.485 CPU sec, 58.607 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666773, 52, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.64, 82.93) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 37, 1059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3141) [M::mem_pestat] mean and std.dev: (475.25, 902.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4182) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.534 CPU sec, 45.879 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 665769, 58, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (304, 415, 2182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5938) [M::mem_pestat] mean and std.dev: (598.90, 618.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7816) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.40, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 37, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1214) [M::mem_pestat] mean and std.dev: (72.39, 142.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1612) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (669, 1186, 1372) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2778) [M::mem_pestat] mean and std.dev: (863.50, 433.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3481) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1038.499 CPU sec, 58.100 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666135, 41, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.51, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 44, 853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2527) [M::mem_pestat] mean and std.dev: (199.18, 554.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3364) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.978 CPU sec, 46.086 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666857, 48, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (358, 423, 1117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2635) [M::mem_pestat] mean and std.dev: (679.00, 529.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3394) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.48, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 40, 292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 836) [M::mem_pestat] mean and std.dev: (75.88, 103.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1108) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1040.550 CPU sec, 59.822 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666598, 45, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.62, 82.96) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 34, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 394) [M::mem_pestat] mean and std.dev: (43.70, 57.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 520) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.727 CPU sec, 46.175 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666768, 52, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (239, 395, 2520) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7082) [M::mem_pestat] mean and std.dev: (1083.36, 1798.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.38, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 45, 1760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5238) [M::mem_pestat] mean and std.dev: (772.08, 1302.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6977) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1030.815 CPU sec, 57.353 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 667318, 48, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 390, 836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2042) [M::mem_pestat] mean and std.dev: (465.13, 380.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2645) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.45, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 42, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 266) [M::mem_pestat] mean and std.dev: (45.58, 38.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 346) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.503 CPU sec, 45.926 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666189, 52, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 1756, 3683) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10585) [M::mem_pestat] mean and std.dev: (2140.20, 2165.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14036) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.47, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 35, 1765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5263) [M::mem_pestat] mean and std.dev: (794.45, 1384.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7012) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1037.052 CPU sec, 57.996 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666600, 51, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.43, 83.00) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 75, 1975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5883) [M::mem_pestat] mean and std.dev: (928.69, 1512.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7837) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.456 CPU sec, 45.900 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666657, 65, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (291, 718, 1475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3843) [M::mem_pestat] mean and std.dev: (845.50, 699.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5027) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.39, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 42, 1785) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5315) [M::mem_pestat] mean and std.dev: (536.84, 1114.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7080) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.774 CPU sec, 58.828 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666705, 44, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (381, 547, 1047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2379) [M::mem_pestat] mean and std.dev: (573.10, 431.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3045) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.46, 82.88) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 41, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (37.24, 32.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 488) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (460, 1078, 1834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4582) [M::mem_pestat] mean and std.dev: (1207.70, 896.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5956) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1037.699 CPU sec, 46.042 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666893, 36, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 253, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 711) [M::mem_pestat] mean and std.dev: (227.40, 118.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.59, 82.97) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 54, 1458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4330) [M::mem_pestat] mean and std.dev: (314.29, 593.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5766) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1038.093 CPU sec, 59.352 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666686, 46, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.51, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 52, 678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1994) [M::mem_pestat] mean and std.dev: (273.73, 521.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2652) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.454 CPU sec, 46.036 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666852, 47, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.61, 82.90) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 34, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 240) [M::mem_pestat] mean and std.dev: (45.35, 54.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 315) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1037.923 CPU sec, 58.742 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667061, 53, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (356, 1008, 2016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5336) [M::mem_pestat] mean and std.dev: (1052.78, 832.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6996) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.30, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 34, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 340) [M::mem_pestat] mean and std.dev: (36.24, 43.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (912, 1284, 1873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3795) [M::mem_pestat] mean and std.dev: (1269.50, 791.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4756) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1040.937 CPU sec, 46.055 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666697, 41, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 1111, 1813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4967) [M::mem_pestat] mean and std.dev: (1261.10, 1344.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.30, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 37, 760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2244) [M::mem_pestat] mean and std.dev: (252.17, 556.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2986) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1034.746 CPU sec, 55.019 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 666615, 44, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.37, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 40, 160) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 456) [M::mem_pestat] mean and std.dev: (49.27, 52.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 604) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1037.599 CPU sec, 58.383 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 666912, 52, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 379, 747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1675) [M::mem_pestat] mean and std.dev: (520.67, 383.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2139) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.56, 82.99) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 36, 833) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2465) [M::mem_pestat] mean and std.dev: (103.88, 207.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3281) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.431 CPU sec, 45.991 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 667332, 53, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.57, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 34, 87) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 229) [M::mem_pestat] mean and std.dev: (32.74, 30.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 300) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1042.064 CPU sec, 57.466 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666598, 51, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (128, 316, 601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1547) [M::mem_pestat] mean and std.dev: (380.70, 269.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2020) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.35, 82.88) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 48, 2617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7817) [M::mem_pestat] mean and std.dev: (1045.12, 1836.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10417) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.496 CPU sec, 45.799 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667159, 51, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (315, 563, 1795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4755) [M::mem_pestat] mean and std.dev: (858.50, 875.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6235) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.62, 82.90) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 35, 97) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259) [M::mem_pestat] mean and std.dev: (33.95, 35.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 340) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (386, 736, 1388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3392) [M::mem_pestat] mean and std.dev: (924.00, 800.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4394) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1033.407 CPU sec, 59.202 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666868, 53, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 322, 747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1739) [M::mem_pestat] mean and std.dev: (384.73, 234.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2235) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.35, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 36, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1326) [M::mem_pestat] mean and std.dev: (101.81, 234.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1762) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.426 CPU sec, 45.978 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666851, 49, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 357, 1001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2455) [M::mem_pestat] mean and std.dev: (508.78, 355.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3182) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.66, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 39, 1061) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3135) [M::mem_pestat] mean and std.dev: (418.96, 865.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4172) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (860, 1018, 1473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2699) [M::mem_pestat] mean and std.dev: (1038.08, 462.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3312) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1039.576 CPU sec, 57.109 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666775, 56, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.68, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 38, 204) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 582) [M::mem_pestat] mean and std.dev: (48.64, 66.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1032.489 CPU sec, 45.799 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666377, 51, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 399, 1676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4522) [M::mem_pestat] mean and std.dev: (703.33, 840.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5945) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.51, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 38, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 544) [M::mem_pestat] mean and std.dev: (65.60, 92.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 720) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1034.653 CPU sec, 57.935 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666659, 45, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.51, 82.87) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 38, 371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1077) [M::mem_pestat] mean and std.dev: (78.30, 131.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1430) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1033.827 CPU sec, 45.790 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666639, 41, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (217, 462, 811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1999) [M::mem_pestat] mean and std.dev: (517.83, 375.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.49, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 37, 135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 373) [M::mem_pestat] mean and std.dev: (42.94, 57.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 492) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.594 CPU sec, 57.157 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666192, 38, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.42, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 37, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 295) [M::mem_pestat] mean and std.dev: (42.23, 45.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 385) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1040.867 CPU sec, 58.526 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666268, 61, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.47, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 39, 212) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 612) [M::mem_pestat] mean and std.dev: (46.96, 75.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.409 CPU sec, 45.979 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666909, 38, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.43, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 47, 713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2103) [M::mem_pestat] mean and std.dev: (183.12, 323.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2798) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1034.622 CPU sec, 57.993 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 667398, 37, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.45, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 37, 256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 726) [M::mem_pestat] mean and std.dev: (56.73, 97.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 961) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1032.726 CPU sec, 45.789 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666612, 41, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.70, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 35, 88) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 226) [M::mem_pestat] mean and std.dev: (40.44, 37.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 295) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1037.765 CPU sec, 57.801 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667340, 42, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (201, 347, 699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1695) [M::mem_pestat] mean and std.dev: (391.82, 328.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2193) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.32, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 61, 1874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5586) [M::mem_pestat] mean and std.dev: (620.86, 1218.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7442) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1029.402 CPU sec, 45.511 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 667153, 46, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.32, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 82, 1428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4232) [M::mem_pestat] mean and std.dev: (473.30, 869.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5634) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1038.407 CPU sec, 58.449 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 666658, 47, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.45, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 26, 59) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 155) [M::mem_pestat] mean and std.dev: (26.52, 21.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 203) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.064 CPU sec, 45.964 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666721, 44, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.71, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 43, 907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2677) [M::mem_pestat] mean and std.dev: (348.72, 755.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3562) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1040.590 CPU sec, 58.740 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667062, 49, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 373, 1184) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3064) [M::mem_pestat] mean and std.dev: (616.30, 554.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.37, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 41, 1889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5629) [M::mem_pestat] mean and std.dev: (815.41, 1454.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7499) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1033.960 CPU sec, 45.773 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 667064, 47, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.41, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 47, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 343) [M::mem_pestat] mean and std.dev: (39.43, 36.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 451) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.287 CPU sec, 57.520 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666618, 61, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.38, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 41, 903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2673) [M::mem_pestat] mean and std.dev: (336.93, 701.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3558) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1037.187 CPU sec, 57.657 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666789, 48, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.41, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 50, 221) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 629) [M::mem_pestat] mean and std.dev: (64.15, 105.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 833) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.760 CPU sec, 46.104 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666621, 49, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.56, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 42, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (39.69, 35.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 511) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.173 CPU sec, 57.977 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666686, 54, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 492, 2546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6990) [M::mem_pestat] mean and std.dev: (1415.38, 1707.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9212) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.54, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 34, 87) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 229) [M::mem_pestat] mean and std.dev: (42.11, 39.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 300) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.147 CPU sec, 46.184 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666975, 35, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.51, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 26, 90) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 248) [M::mem_pestat] mean and std.dev: (28.11, 27.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 327) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.010 CPU sec, 57.808 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666848, 44, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 318, 593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1363) [M::mem_pestat] mean and std.dev: (409.50, 336.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1754) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.34, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 40, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 879) [M::mem_pestat] mean and std.dev: (105.00, 183.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1164) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.185 CPU sec, 46.045 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666825, 37, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.44, 82.85) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 34, 94) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 254) [M::mem_pestat] mean and std.dev: (38.84, 40.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1041.356 CPU sec, 57.936 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667184, 53, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (211, 317, 431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 871) [M::mem_pestat] mean and std.dev: (323.00, 192.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.34, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 36, 183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 511) [M::mem_pestat] mean and std.dev: (51.50, 74.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 675) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1032.747 CPU sec, 45.629 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667041, 45, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 365, 834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2064) [M::mem_pestat] mean and std.dev: (595.00, 551.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2802) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.39, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 27, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 502) [M::mem_pestat] mean and std.dev: (50.50, 84.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 666) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1038.107 CPU sec, 58.147 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666975, 58, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.45, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 39, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (86.77, 147.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1143) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1038.552 CPU sec, 46.000 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666434, 65, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.55, 82.94) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 33, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 332) [M::mem_pestat] mean and std.dev: (45.50, 56.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 437) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.612 CPU sec, 57.265 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666526, 54, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 419, 967) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2373) [M::mem_pestat] mean and std.dev: (649.90, 589.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3076) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.57, 82.94) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 37, 1829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5449) [M::mem_pestat] mean and std.dev: (815.65, 1441.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7259) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.751 CPU sec, 46.009 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666282, 41, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (319, 358, 1147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2803) [M::mem_pestat] mean and std.dev: (764.70, 622.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.46, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 25, 60) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 150) [M::mem_pestat] mean and std.dev: (25.33, 16.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 195) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1039.075 CPU sec, 57.427 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 667136, 49, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 896, 4311) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12507) [M::mem_pestat] mean and std.dev: (2010.69, 2701.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.65, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 35, 452) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1318) [M::mem_pestat] mean and std.dev: (180.20, 327.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1751) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1034.695 CPU sec, 45.761 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666676, 46, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (333, 404, 2180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5874) [M::mem_pestat] mean and std.dev: (756.00, 779.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7721) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.55, 82.92) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 37, 1240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3696) [M::mem_pestat] mean and std.dev: (476.81, 839.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4924) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1035.291 CPU sec, 57.089 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 667165, 53, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.46, 83.05) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 32, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 172) [M::mem_pestat] mean and std.dev: (31.16, 21.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 224) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1029.291 CPU sec, 45.653 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666777, 34, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.49, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 31, 41) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 97) [M::mem_pestat] mean and std.dev: (26.10, 17.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 125) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1031.721 CPU sec, 56.552 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667462, 53, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (79, 206, 1069) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3049) [M::mem_pestat] mean and std.dev: (475.27, 566.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4039) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.52, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 30, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 282) [M::mem_pestat] mean and std.dev: (29.69, 24.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 370) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1026.705 CPU sec, 45.577 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667047, 57, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (90, 858, 1601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4623) [M::mem_pestat] mean and std.dev: (928.00, 996.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6134) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.67, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 42, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 210) [M::mem_pestat] mean and std.dev: (45.62, 44.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 275) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1041.954 CPU sec, 58.370 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 667371, 44, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.70, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 37, 862) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2560) [M::mem_pestat] mean and std.dev: (195.94, 544.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3409) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1029.185 CPU sec, 45.574 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 667174, 55, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.42, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 30, 258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 744) [M::mem_pestat] mean and std.dev: (50.38, 87.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 987) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1034.524 CPU sec, 57.062 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666875, 46, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (83, 246, 628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1718) [M::mem_pestat] mean and std.dev: (348.70, 353.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2263) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.55, 82.97) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 65, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1319) [M::mem_pestat] mean and std.dev: (95.36, 136.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1752) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.505 CPU sec, 46.083 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666430, 67, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (246, 413, 532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1104) [M::mem_pestat] mean and std.dev: (398.22, 241.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.62, 82.88) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 38, 206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (48.94, 58.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1044.296 CPU sec, 52.789 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666886, 40, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (161, 425, 935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2483) [M::mem_pestat] mean and std.dev: (398.36, 312.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3257) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.65, 83.02) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 46, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 316) [M::mem_pestat] mean and std.dev: (45.22, 38.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 411) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1051.421 CPU sec, 61.309 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 667065, 45, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.43, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 42, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (53.23, 65.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1048.131 CPU sec, 46.548 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666761, 59, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (352, 427, 1058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2470) [M::mem_pestat] mean and std.dev: (595.69, 432.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3176) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.45, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 27, 61) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 153) [M::mem_pestat] mean and std.dev: (32.59, 28.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 199) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1036.712 CPU sec, 58.946 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667063, 55, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (131, 272, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1247) [M::mem_pestat] mean and std.dev: (280.90, 227.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1619) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.39, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 41, 1191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3539) [M::mem_pestat] mean and std.dev: (512.02, 928.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4713) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1044.012 CPU sec, 46.273 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 667266, 56, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (152, 267, 564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1388) [M::mem_pestat] mean and std.dev: (288.36, 186.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.43, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 42, 619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1815) [M::mem_pestat] mean and std.dev: (103.22, 260.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2413) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1038.258 CPU sec, 59.506 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666646, 54, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.46, 82.94) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 43, 659) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1937) [M::mem_pestat] mean and std.dev: (138.98, 361.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2576) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (747, 1335, 1556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3174) [M::mem_pestat] mean and std.dev: (1013.11, 532.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3983) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1041.040 CPU sec, 46.193 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666586, 42, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (242, 290, 344) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (38, 548) [M::mem_pestat] mean and std.dev: (256.40, 93.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.42, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 51, 2103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6269) [M::mem_pestat] mean and std.dev: (719.18, 1410.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8352) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1039.726 CPU sec, 59.276 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666825, 49, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (344, 1285, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7121) [M::mem_pestat] mean and std.dev: (1172.91, 978.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.47, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 33, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 281) [M::mem_pestat] mean and std.dev: (44.95, 50.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 369) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.379 CPU sec, 45.756 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666876, 49, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.37, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 40, 823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2429) [M::mem_pestat] mean and std.dev: (243.28, 553.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3232) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1039.561 CPU sec, 58.686 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 667385, 43, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.30, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 44, 1556) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4620) [M::mem_pestat] mean and std.dev: (709.40, 1251.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6152) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1034.394 CPU sec, 45.794 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 667054, 61, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (262, 755, 1050) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2626) [M::mem_pestat] mean and std.dev: (687.10, 508.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3414) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.32, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 34, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 387) [M::mem_pestat] mean and std.dev: (42.27, 38.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 509) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1031.184 CPU sec, 56.431 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666693, 55, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (417, 461, 898) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1860) [M::mem_pestat] mean and std.dev: (665.82, 345.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2341) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.68, 82.96) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 28, 77) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 199) [M::mem_pestat] mean and std.dev: (32.25, 25.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 260) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1042.797 CPU sec, 59.813 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 666561, 38, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.68, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 44, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 300) [M::mem_pestat] mean and std.dev: (37.10, 25.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 393) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1040.470 CPU sec, 46.115 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 666990, 51, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.55, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 28, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1460) [M::mem_pestat] mean and std.dev: (108.41, 238.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1942) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1032.864 CPU sec, 57.936 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666636, 51, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (51, 284, 1555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4563) [M::mem_pestat] mean and std.dev: (591.44, 690.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6067) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.35, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 57, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 378) [M::mem_pestat] mean and std.dev: (44.51, 32.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 496) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1034.140 CPU sec, 45.893 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 666597, 42, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (238, 336, 952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2380) [M::mem_pestat] mean and std.dev: (400.33, 335.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3094) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.47, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 41, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 240) [M::mem_pestat] mean and std.dev: (38.91, 30.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 312) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1040.228 CPU sec, 58.660 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666998, 46, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.63, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 31, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 202) [M::mem_pestat] mean and std.dev: (27.97, 19.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 265) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1046.014 CPU sec, 46.301 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666451, 59, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.46, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 36, 1381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4113) [M::mem_pestat] mean and std.dev: (377.17, 706.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5479) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1042.705 CPU sec, 58.173 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666242, 59, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 568, 1981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5561) [M::mem_pestat] mean and std.dev: (1110.31, 1290.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.54, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 35, 1448) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4310) [M::mem_pestat] mean and std.dev: (503.10, 997.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5741) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1042.674 CPU sec, 46.295 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666807, 47, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (82, 179, 441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1159) [M::mem_pestat] mean and std.dev: (185.33, 140.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1518) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.60, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 35, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (45.15, 46.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1036.813 CPU sec, 58.293 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 666351, 66, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (308, 409, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 830) [M::mem_pestat] mean and std.dev: (380.82, 161.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1027) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.66, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 60, 1077) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3187) [M::mem_pestat] mean and std.dev: (337.78, 560.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4242) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.198 CPU sec, 46.249 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 667001, 34, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.56, 82.94) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 34, 92) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 246) [M::mem_pestat] mean and std.dev: (31.46, 24.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 323) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1041.540 CPU sec, 59.731 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 666543, 46, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (335, 577, 2109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5657) [M::mem_pestat] mean and std.dev: (558.50, 559.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7431) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.39, 82.89) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 28, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 328) [M::mem_pestat] mean and std.dev: (47.20, 62.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1054.847 CPU sec, 60.481 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 666278, 49, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (268, 352, 777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1795) [M::mem_pestat] mean and std.dev: (525.23, 411.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 665) [M::mem_pestat] mean and std.dev: (393.52, 82.96) [M::mem_pestat] low and high boundaries for proper pairs: (11, 774) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 44, 1177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3495) [M::mem_pestat] mean and std.dev: (407.82, 788.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4654) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1050.582 CPU sec, 46.652 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 666602, 46, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.62, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 51, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 458) [M::mem_pestat] mean and std.dev: (54.25, 56.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 603) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1044.786 CPU sec, 59.819 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 666645, 42, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.43, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 52, 1445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4289) [M::mem_pestat] mean and std.dev: (778.95, 1260.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5821) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.055 CPU sec, 46.098 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 666955, 56, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (212, 426, 843) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2105) [M::mem_pestat] mean and std.dev: (451.36, 449.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.46, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 34, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 887) [M::mem_pestat] mean and std.dev: (55.51, 98.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1178) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1041.265 CPU sec, 58.986 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 666672, 41, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.44, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 46, 516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1514) [M::mem_pestat] mean and std.dev: (176.89, 332.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2013) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1043.204 CPU sec, 46.191 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 666895, 48, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.42, 82.45) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 38, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (33.79, 27.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 417) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1042.317 CPU sec, 58.214 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666513, 52, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 280, 1302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3476) [M::mem_pestat] mean and std.dev: (705.94, 631.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 660) [M::mem_pestat] mean and std.dev: (393.53, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (18, 767) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 47, 1959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5837) [M::mem_pestat] mean and std.dev: (760.96, 1464.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7776) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1048.905 CPU sec, 46.401 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 666884, 60, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 377, 1402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3608) [M::mem_pestat] mean and std.dev: (592.58, 460.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4711) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.74, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 49, 747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2203) [M::mem_pestat] mean and std.dev: (167.39, 340.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2931) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1037.421 CPU sec, 57.876 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 667179, 50, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (146, 572, 1134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3110) [M::mem_pestat] mean and std.dev: (538.30, 470.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4098) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.39, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 29, 63) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 159) [M::mem_pestat] mean and std.dev: (30.07, 26.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 207) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1037.592 CPU sec, 45.886 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 666359, 55, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (287, 407, 1290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3296) [M::mem_pestat] mean and std.dev: (747.71, 550.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4299) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (339, 390, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 663) [M::mem_pestat] mean and std.dev: (393.70, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (15, 771) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 43, 1158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3430) [M::mem_pestat] mean and std.dev: (169.19, 453.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4566) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1039.668 CPU sec, 58.141 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 667110, 54, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 662) [M::mem_pestat] mean and std.dev: (393.44, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (14, 770) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 66, 1410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4194) [M::mem_pestat] mean and std.dev: (517.16, 921.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5586) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1036.750 CPU sec, 45.937 real sec [M::process] read 38830 sequences (5824500 bp)... [M::process] 0 single-end sequences; 38830 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (1, 16112, 2, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (338, 389, 445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 659) [M::mem_pestat] mean and std.dev: (392.54, 82.28) [M::mem_pestat] low and high boundaries for proper pairs: (17, 766) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 38830 reads in 25.976 CPU sec, 1.183 real sec [main] Version: 0.7.17-r1188 [main] CMD: /home/csyk2proj/tools/bwa.kit/bwa mem -p -t24 -R@RG\tID:KPGP-00340\tPL:ILLUMINA\tSM:KPGP-00340 -C /home/csyk2proj/data/bwa.kit/hs38DH.fa - [main] Real time: 34160.912 sec; CPU: 412993.776 sec