[M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643020, 41, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (384, 494, 821) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1695) [M::mem_pestat] mean and std.dev: (519.09, 270.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2132) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.06, 97.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 46, 1750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5214) [M::mem_pestat] mean and std.dev: (765.15, 1374.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6946) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1184.883 CPU sec, 68.012 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 643335, 37, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.88, 97.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 43, 668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1970) [M::mem_pestat] mean and std.dev: (146.19, 316.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2621) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1167.868 CPU sec, 51.709 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 642433, 47, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (420, 518, 1588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3924) [M::mem_pestat] mean and std.dev: (797.67, 524.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5092) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.08, 97.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 58, 4376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13066) [M::mem_pestat] mean and std.dev: (1991.02, 2925.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17411) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1176.480 CPU sec, 63.466 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643270, 55, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (414, 610, 1818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4626) [M::mem_pestat] mean and std.dev: (1020.09, 743.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6030) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.89, 97.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 106, 1827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5433) [M::mem_pestat] mean and std.dev: (852.83, 1280.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7236) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1165.422 CPU sec, 51.390 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 642709, 33, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (379, 596, 1903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4951) [M::mem_pestat] mean and std.dev: (984.85, 790.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.03, 97.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 37, 1735) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5175) [M::mem_pestat] mean and std.dev: (567.87, 1081.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6895) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (985, 1335, 2375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5155) [M::mem_pestat] mean and std.dev: (1515.00, 631.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6545) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1160.656 CPU sec, 62.045 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643053, 58, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (359, 478, 1664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4274) [M::mem_pestat] mean and std.dev: (919.46, 1049.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.96, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 152, 2149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6403) [M::mem_pestat] mean and std.dev: (1082.89, 1698.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8530) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1168.217 CPU sec, 51.544 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643262, 43, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (380, 709, 1509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3767) [M::mem_pestat] mean and std.dev: (809.18, 502.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.00, 97.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 48, 1603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4783) [M::mem_pestat] mean and std.dev: (699.56, 1181.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6373) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1176.078 CPU sec, 67.573 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643120, 48, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (293, 609, 1045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2549) [M::mem_pestat] mean and std.dev: (852.50, 817.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4121) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.15, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 106, 845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2495) [M::mem_pestat] mean and std.dev: (255.61, 477.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3320) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1159.768 CPU sec, 51.177 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643303, 42, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.84, 96.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 45, 743) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2193) [M::mem_pestat] mean and std.dev: (190.63, 372.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2918) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1160.273 CPU sec, 57.883 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643326, 51, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.95, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 48, 1026) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3028) [M::mem_pestat] mean and std.dev: (431.24, 777.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4029) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1166.241 CPU sec, 65.904 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 642833, 52, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (461, 1364, 2362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6164) [M::mem_pestat] mean and std.dev: (1249.08, 982.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8065) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.00, 97.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 52, 2080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6208) [M::mem_pestat] mean and std.dev: (1037.49, 1476.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8272) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1168.356 CPU sec, 51.809 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 642942, 43, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (354, 616, 1002) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2298) [M::mem_pestat] mean and std.dev: (540.50, 238.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2946) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.09, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 183, 2873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8569) [M::mem_pestat] mean and std.dev: (1652.38, 2138.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11417) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1178.683 CPU sec, 68.613 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 643012, 57, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.08, 97.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (38, 119, 1526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4502) [M::mem_pestat] mean and std.dev: (783.33, 1397.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6373) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1164.596 CPU sec, 51.583 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 642478, 50, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (251, 413, 1115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2843) [M::mem_pestat] mean and std.dev: (618.69, 543.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.06, 97.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 62, 2619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7793) [M::mem_pestat] mean and std.dev: (1151.04, 1700.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10380) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1174.997 CPU sec, 68.142 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643187, 50, 14) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.18, 97.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 79, 2206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6572) [M::mem_pestat] mean and std.dev: (1030.15, 1574.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8755) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (279, 766, 2251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6195) [M::mem_pestat] mean and std.dev: (981.42, 1145.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8167) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1160.554 CPU sec, 51.447 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643347, 38, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (357, 476, 1796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4674) [M::mem_pestat] mean and std.dev: (724.67, 600.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6113) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.01, 97.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 100, 1447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4285) [M::mem_pestat] mean and std.dev: (596.79, 1142.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5704) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (781, 1219, 1709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3565) [M::mem_pestat] mean and std.dev: (1225.86, 667.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4493) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1166.909 CPU sec, 66.953 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 642846, 39, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (550, 702, 1752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4156) [M::mem_pestat] mean and std.dev: (1018.15, 664.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5358) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.17, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 57, 2274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6774) [M::mem_pestat] mean and std.dev: (1326.76, 1785.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9024) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1159.388 CPU sec, 51.187 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643002, 49, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (407, 652, 3235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8891) [M::mem_pestat] mean and std.dev: (1986.50, 2302.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.82, 97.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 61, 647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1881) [M::mem_pestat] mean and std.dev: (260.65, 461.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2498) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1157.348 CPU sec, 62.742 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643093, 56, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (349, 530, 863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1891) [M::mem_pestat] mean and std.dev: (596.23, 363.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.86, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 93, 1466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4338) [M::mem_pestat] mean and std.dev: (394.40, 696.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5774) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1163.517 CPU sec, 66.977 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642863, 55, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (260, 471, 2978) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8414) [M::mem_pestat] mean and std.dev: (1132.20, 1330.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11132) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.94, 97.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 86, 1575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4679) [M::mem_pestat] mean and std.dev: (647.12, 1170.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6231) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1158.008 CPU sec, 51.220 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643678, 46, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.88, 97.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 245, 3194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9504) [M::mem_pestat] mean and std.dev: (1858.61, 2459.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12659) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (906, 1479, 5886) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 15846) [M::mem_pestat] mean and std.dev: (2713.00, 2476.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 20826) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1153.909 CPU sec, 64.847 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 642875, 54, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 355, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1037) [M::mem_pestat] mean and std.dev: (349.67, 162.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.31, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 41, 830) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2458) [M::mem_pestat] mean and std.dev: (231.91, 472.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3272) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1168.212 CPU sec, 51.660 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643112, 47, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (326, 394, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 815) [M::mem_pestat] mean and std.dev: (360.11, 108.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 978) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.10, 97.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 82, 2606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7776) [M::mem_pestat] mean and std.dev: (1132.66, 1778.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10361) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1164.387 CPU sec, 66.782 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 643352, 51, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (442, 676, 1342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3142) [M::mem_pestat] mean and std.dev: (823.53, 550.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4042) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.11, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 42, 1402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4162) [M::mem_pestat] mean and std.dev: (362.91, 750.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5542) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1153.033 CPU sec, 51.049 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 642500, 49, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (452, 1013, 1322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3062) [M::mem_pestat] mean and std.dev: (984.25, 701.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3932) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.07, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 88, 1647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4903) [M::mem_pestat] mean and std.dev: (807.91, 1425.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6531) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1160.645 CPU sec, 65.626 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643526, 50, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.92, 97.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 86, 1582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4690) [M::mem_pestat] mean and std.dev: (694.76, 1161.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6244) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1150.914 CPU sec, 50.882 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643113, 42, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (447, 851, 2748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7350) [M::mem_pestat] mean and std.dev: (1452.07, 1256.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9651) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.00, 97.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 69, 781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2317) [M::mem_pestat] mean and std.dev: (238.11, 447.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3085) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1043, 1397, 1563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (3, 2603) [M::mem_pestat] mean and std.dev: (1309.00, 354.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3123) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1166.444 CPU sec, 64.191 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 642350, 43, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 487, 1033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2545) [M::mem_pestat] mean and std.dev: (514.83, 378.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.10, 97.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 138, 2559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7617) [M::mem_pestat] mean and std.dev: (1182.54, 1888.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10146) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1170.076 CPU sec, 66.062 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 642933, 44, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.00, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 53, 2435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7267) [M::mem_pestat] mean and std.dev: (1224.02, 1901.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9683) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1154.702 CPU sec, 51.128 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643156, 46, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 406, 528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1084) [M::mem_pestat] mean and std.dev: (318.73, 147.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.91, 97.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 45, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 700) [M::mem_pestat] mean and std.dev: (61.97, 84.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1162.049 CPU sec, 66.557 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 642698, 49, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (361, 772, 1832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4774) [M::mem_pestat] mean and std.dev: (870.43, 650.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6245) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.02, 96.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (49, 218, 3617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10753) [M::mem_pestat] mean and std.dev: (1622.61, 2283.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14321) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1155.516 CPU sec, 51.291 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643266, 40, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (294, 783, 1741) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4635) [M::mem_pestat] mean and std.dev: (1040.25, 1005.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6082) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.93, 97.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 52, 1598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4746) [M::mem_pestat] mean and std.dev: (595.17, 1133.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6320) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (371, 796, 950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2108) [M::mem_pestat] mean and std.dev: (626.56, 333.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2687) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1153.441 CPU sec, 64.832 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643315, 45, 14) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.16, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 47, 495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1447) [M::mem_pestat] mean and std.dev: (98.87, 162.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1923) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (777, 902, 2745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6681) [M::mem_pestat] mean and std.dev: (1561.21, 1190.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8649) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1160.573 CPU sec, 51.251 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 642790, 68, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.89, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 83, 1693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5037) [M::mem_pestat] mean and std.dev: (828.08, 1278.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6709) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (809, 998, 1129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (169, 1769) [M::mem_pestat] mean and std.dev: (863.75, 250.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2089) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1150.498 CPU sec, 64.715 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 642989, 39, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (493, 1247, 2118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5368) [M::mem_pestat] mean and std.dev: (1164.27, 909.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6993) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.13, 97.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 56, 2660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7926) [M::mem_pestat] mean and std.dev: (1240.18, 1885.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10559) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1158.159 CPU sec, 51.208 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643104, 60, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (285, 470, 2969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8337) [M::mem_pestat] mean and std.dev: (1407.20, 1686.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11021) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.91, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 59, 937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2773) [M::mem_pestat] mean and std.dev: (291.83, 626.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3691) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (982, 2632, 3845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9571) [M::mem_pestat] mean and std.dev: (2704.90, 1939.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12434) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1163.500 CPU sec, 65.368 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 643461, 42, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.97, 97.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 134, 1231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3635) [M::mem_pestat] mean and std.dev: (592.46, 868.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4837) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1157.104 CPU sec, 65.856 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 642678, 40, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 427, 1180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2908) [M::mem_pestat] mean and std.dev: (580.87, 449.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.12, 97.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 71, 1270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3766) [M::mem_pestat] mean and std.dev: (421.31, 807.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5014) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1157.998 CPU sec, 51.237 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 642984, 45, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.86, 96.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 96, 1547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4577) [M::mem_pestat] mean and std.dev: (613.67, 1124.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6092) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (917, 1130, 2009) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4193) [M::mem_pestat] mean and std.dev: (1501.92, 979.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5420) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1163.856 CPU sec, 66.953 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643073, 64, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (344, 561, 2070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5522) [M::mem_pestat] mean and std.dev: (977.82, 904.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7248) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.87, 97.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 48, 1282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3814) [M::mem_pestat] mean and std.dev: (380.78, 786.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5080) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (560, 830, 1425) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3155) [M::mem_pestat] mean and std.dev: (713.75, 416.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4020) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1157.827 CPU sec, 51.421 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642791, 51, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (489, 800, 920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1782) [M::mem_pestat] mean and std.dev: (675.50, 306.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2213) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.05, 97.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 59, 2428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7246) [M::mem_pestat] mean and std.dev: (996.58, 1605.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9655) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1159.138 CPU sec, 66.482 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643451, 42, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.95, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 59, 3861) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11523) [M::mem_pestat] mean and std.dev: (1625.14, 2141.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15354) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (422, 1910, 2923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7925) [M::mem_pestat] mean and std.dev: (1415.55, 1090.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10426) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1155.289 CPU sec, 51.135 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 642989, 54, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 426, 1053) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2561) [M::mem_pestat] mean and std.dev: (702.15, 522.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3315) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.96, 97.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 46, 1163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3461) [M::mem_pestat] mean and std.dev: (405.94, 794.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4610) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1155.667 CPU sec, 66.021 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 643223, 41, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (396, 718, 1082) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2454) [M::mem_pestat] mean and std.dev: (717.93, 417.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3140) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.14, 97.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 78, 1341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3979) [M::mem_pestat] mean and std.dev: (424.41, 862.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5298) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1153.832 CPU sec, 51.185 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643104, 47, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (275, 420, 643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1379) [M::mem_pestat] mean and std.dev: (335.20, 182.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1747) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.89, 96.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 47, 1634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4858) [M::mem_pestat] mean and std.dev: (729.89, 1441.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6498) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1152.306 CPU sec, 65.754 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 642798, 40, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (364, 1246, 1447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3613) [M::mem_pestat] mean and std.dev: (961.64, 533.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4696) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.17, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 56, 222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 632) [M::mem_pestat] mean and std.dev: (73.42, 90.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 837) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (913, 1095, 1323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (93, 2143) [M::mem_pestat] mean and std.dev: (1216.30, 368.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2690) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1152.646 CPU sec, 50.999 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643147, 53, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.00, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 44, 478) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1392) [M::mem_pestat] mean and std.dev: (123.44, 271.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1849) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1163.884 CPU sec, 67.586 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 642652, 46, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.22, 97.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 92, 2872) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8584) [M::mem_pestat] mean and std.dev: (1471.02, 2288.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11440) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1159.993 CPU sec, 51.606 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 642723, 50, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.03, 97.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 98, 1156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3404) [M::mem_pestat] mean and std.dev: (422.21, 734.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4528) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (578, 1220, 2391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6017) [M::mem_pestat] mean and std.dev: (1386.30, 1013.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7830) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1163.799 CPU sec, 67.260 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 642922, 44, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (449, 935, 3624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9974) [M::mem_pestat] mean and std.dev: (1860.60, 1863.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13149) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.96, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 84, 1906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5654) [M::mem_pestat] mean and std.dev: (992.63, 1396.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7528) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1160.684 CPU sec, 51.404 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 643321, 54, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (387, 657, 1302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3132) [M::mem_pestat] mean and std.dev: (900.00, 796.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4087) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.11, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 100, 1279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3779) [M::mem_pestat] mean and std.dev: (570.82, 928.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5029) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1158.120 CPU sec, 66.150 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 643004, 53, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (395, 546, 970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2120) [M::mem_pestat] mean and std.dev: (602.71, 446.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.80, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 52, 2581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7699) [M::mem_pestat] mean and std.dev: (1338.02, 2098.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10258) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1150.005 CPU sec, 50.987 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 642957, 43, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.14, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 234, 2608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7758) [M::mem_pestat] mean and std.dev: (1044.74, 1672.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10333) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1154.692 CPU sec, 66.780 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643821, 44, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 341, 641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1395) [M::mem_pestat] mean and std.dev: (495.71, 375.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1998) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.94, 97.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 75, 1297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3831) [M::mem_pestat] mean and std.dev: (459.48, 785.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5098) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (881, 1103, 1523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2807) [M::mem_pestat] mean and std.dev: (1229.20, 551.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3449) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1155.208 CPU sec, 51.145 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643350, 49, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (318, 601, 640) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1284) [M::mem_pestat] mean and std.dev: (461.75, 158.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1606) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.98, 97.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 46, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2174) [M::mem_pestat] mean and std.dev: (199.12, 414.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2893) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1156.502 CPU sec, 59.005 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643285, 48, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (328, 499, 2266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6142) [M::mem_pestat] mean and std.dev: (1121.00, 947.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8080) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.92, 97.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 56, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 575) [M::mem_pestat] mean and std.dev: (54.47, 54.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 759) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1167.488 CPU sec, 66.533 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 643015, 43, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.93, 97.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 94, 2630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7832) [M::mem_pestat] mean and std.dev: (1267.69, 1773.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10433) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1161.216 CPU sec, 51.540 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 642353, 43, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.92, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 59, 1696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5044) [M::mem_pestat] mean and std.dev: (849.29, 1518.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6922) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1162.946 CPU sec, 67.196 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643424, 47, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (393, 498, 1051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2367) [M::mem_pestat] mean and std.dev: (597.82, 386.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3025) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.81, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 107, 3113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9271) [M::mem_pestat] mean and std.dev: (1684.62, 2570.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12350) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1159.238 CPU sec, 51.479 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 643591, 45, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (435, 707, 1371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3243) [M::mem_pestat] mean and std.dev: (878.44, 620.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4179) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.83, 97.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 156, 3300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9828) [M::mem_pestat] mean and std.dev: (1761.49, 2512.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13092) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1160.767 CPU sec, 67.473 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643741, 40, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (379, 862, 959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2119) [M::mem_pestat] mean and std.dev: (723.00, 499.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2719) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.88, 97.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 107, 1392) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4114) [M::mem_pestat] mean and std.dev: (450.77, 850.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5475) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1150.189 CPU sec, 50.712 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 642771, 48, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (389, 458, 1526) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3800) [M::mem_pestat] mean and std.dev: (889.67, 870.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4937) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.13, 97.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 158, 1877) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5585) [M::mem_pestat] mean and std.dev: (867.33, 1293.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7439) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1166.671 CPU sec, 66.851 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 643401, 53, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.15, 96.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 39, 1146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3418) [M::mem_pestat] mean and std.dev: (339.83, 670.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4554) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1152.072 CPU sec, 50.826 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642833, 50, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (233, 436, 1175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3059) [M::mem_pestat] mean and std.dev: (562.80, 425.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4001) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.01, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 57, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (46.82, 32.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 344) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1168.723 CPU sec, 63.625 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 643272, 49, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.76, 97.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 39, 697) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2059) [M::mem_pestat] mean and std.dev: (232.24, 462.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2740) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (693, 1457, 4498) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12108) [M::mem_pestat] mean and std.dev: (1931.08, 1822.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15913) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1167.509 CPU sec, 68.395 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 642944, 40, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (218, 445, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1946) [M::mem_pestat] mean and std.dev: (356.30, 223.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2522) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.14, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 213, 1612) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4784) [M::mem_pestat] mean and std.dev: (870.46, 1298.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6370) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1166.845 CPU sec, 51.872 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643371, 59, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (423, 546, 1531) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3747) [M::mem_pestat] mean and std.dev: (915.55, 824.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4855) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.07, 97.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 85, 2153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6409) [M::mem_pestat] mean and std.dev: (821.36, 1500.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8537) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1221, 1381, 2745) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5793) [M::mem_pestat] mean and std.dev: (1569.40, 1136.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1171.728 CPU sec, 66.927 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643315, 44, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (350, 643, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2771) [M::mem_pestat] mean and std.dev: (751.50, 468.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.06, 97.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 142, 2961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8851) [M::mem_pestat] mean and std.dev: (1604.52, 2383.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11796) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (405, 1055, 1064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2382) [M::mem_pestat] mean and std.dev: (713.89, 424.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3041) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1167.920 CPU sec, 51.716 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 642944, 56, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 426, 792) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1752) [M::mem_pestat] mean and std.dev: (499.15, 294.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2232) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.86, 96.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 69, 1144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3402) [M::mem_pestat] mean and std.dev: (424.33, 762.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4531) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1175.260 CPU sec, 68.270 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643780, 37, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.18, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 38, 1734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5176) [M::mem_pestat] mean and std.dev: (712.57, 1162.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6897) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1157.878 CPU sec, 51.155 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643363, 44, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (464, 555, 1852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4628) [M::mem_pestat] mean and std.dev: (1347.54, 1368.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6822) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.87, 97.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 116, 774) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2284) [M::mem_pestat] mean and std.dev: (293.13, 441.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3039) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1176.050 CPU sec, 68.608 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643707, 48, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (449, 642, 1107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2423) [M::mem_pestat] mean and std.dev: (714.82, 394.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3081) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.93, 97.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 140, 2181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6489) [M::mem_pestat] mean and std.dev: (1181.17, 1848.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8643) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1166.303 CPU sec, 51.627 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 642943, 46, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.99, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 40, 1755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5231) [M::mem_pestat] mean and std.dev: (683.83, 1214.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6969) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (811, 1161, 1421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2641) [M::mem_pestat] mean and std.dev: (1060.00, 319.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3251) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1173.930 CPU sec, 67.858 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 642955, 42, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (284, 355, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 971) [M::mem_pestat] mean and std.dev: (340.00, 130.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1200) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.05, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 67, 1098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3250) [M::mem_pestat] mean and std.dev: (496.97, 837.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4326) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (853, 1669, 3458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8668) [M::mem_pestat] mean and std.dev: (2213.75, 2318.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11488) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1175.251 CPU sec, 67.554 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 643254, 45, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (316, 772, 1393) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3547) [M::mem_pestat] mean and std.dev: (831.88, 629.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.05, 97.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 45, 1149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3415) [M::mem_pestat] mean and std.dev: (501.45, 907.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4548) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (614, 1080, 1739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3989) [M::mem_pestat] mean and std.dev: (1036.08, 617.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5114) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1165.244 CPU sec, 51.608 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 642937, 47, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (273, 383, 1072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2670) [M::mem_pestat] mean and std.dev: (766.07, 623.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3469) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.97, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 53, 1397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4125) [M::mem_pestat] mean and std.dev: (527.19, 972.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5489) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1168.681 CPU sec, 67.732 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642844, 50, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (288, 367, 916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2172) [M::mem_pestat] mean and std.dev: (562.36, 401.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2800) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.89, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 69, 1482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4406) [M::mem_pestat] mean and std.dev: (653.04, 1102.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5868) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (276, 912, 1222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3114) [M::mem_pestat] mean and std.dev: (916.00, 627.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4060) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1168.674 CPU sec, 51.841 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 642604, 49, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 664, 921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2333) [M::mem_pestat] mean and std.dev: (610.10, 409.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3039) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.98, 96.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 59, 1724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5130) [M::mem_pestat] mean and std.dev: (791.92, 1345.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6833) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (878, 1573, 4074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10466) [M::mem_pestat] mean and std.dev: (2132.77, 1707.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13662) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1180.425 CPU sec, 68.613 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643280, 39, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.17, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 104, 1688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5016) [M::mem_pestat] mean and std.dev: (896.34, 1387.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6680) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1160.167 CPU sec, 51.216 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643546, 52, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.97, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 154, 2511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7475) [M::mem_pestat] mean and std.dev: (1162.00, 1929.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9957) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1167.343 CPU sec, 67.792 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 642342, 46, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (206, 423, 982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2534) [M::mem_pestat] mean and std.dev: (559.60, 385.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3310) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.89, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 133, 2624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7826) [M::mem_pestat] mean and std.dev: (1235.81, 2094.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10427) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1161.504 CPU sec, 51.526 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643299, 38, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (414, 467, 1447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3513) [M::mem_pestat] mean and std.dev: (929.18, 796.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4546) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.09, 97.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 123, 631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1837) [M::mem_pestat] mean and std.dev: (223.94, 341.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2440) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1172.023 CPU sec, 68.284 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 642757, 41, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.27, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 28, 606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1794) [M::mem_pestat] mean and std.dev: (112.74, 239.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2388) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (249, 570, 1300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3402) [M::mem_pestat] mean and std.dev: (715.85, 526.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4453) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1168.726 CPU sec, 51.891 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642952, 49, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (403, 421, 2140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5614) [M::mem_pestat] mean and std.dev: (1078.18, 1292.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.02, 96.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 75, 1321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3931) [M::mem_pestat] mean and std.dev: (449.54, 754.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5236) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (631, 859, 1276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2566) [M::mem_pestat] mean and std.dev: (906.08, 522.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3211) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1170.919 CPU sec, 68.250 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643446, 45, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (300, 572, 1274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3222) [M::mem_pestat] mean and std.dev: (708.10, 470.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4196) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.20, 96.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 166, 2097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6217) [M::mem_pestat] mean and std.dev: (883.66, 1319.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8277) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1169.828 CPU sec, 51.683 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642646, 39, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (699, 959, 1426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2880) [M::mem_pestat] mean and std.dev: (992.45, 522.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.90, 97.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 37, 111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (36.65, 35.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 402) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1170.627 CPU sec, 68.886 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643042, 44, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (431, 1065, 1505) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3653) [M::mem_pestat] mean and std.dev: (890.44, 459.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4727) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.11, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 48, 2128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6340) [M::mem_pestat] mean and std.dev: (894.16, 1622.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8446) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (451, 690, 1516) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3646) [M::mem_pestat] mean and std.dev: (1049.23, 846.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4711) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1165.824 CPU sec, 51.626 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643091, 52, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (361, 563, 1664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4270) [M::mem_pestat] mean and std.dev: (764.09, 558.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.91, 97.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 246, 2642) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7876) [M::mem_pestat] mean and std.dev: (1254.80, 1761.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10493) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1165.906 CPU sec, 67.872 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 642780, 47, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (382, 508, 786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1594) [M::mem_pestat] mean and std.dev: (447.10, 197.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1998) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.95, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 70, 1649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4899) [M::mem_pestat] mean and std.dev: (737.88, 1255.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6524) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1165.428 CPU sec, 51.600 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 642469, 48, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (377, 842, 1326) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3224) [M::mem_pestat] mean and std.dev: (654.64, 410.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4173) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.84, 97.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 51, 1783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5299) [M::mem_pestat] mean and std.dev: (672.20, 1235.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7057) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1168.480 CPU sec, 67.152 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643632, 47, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.98, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 153, 1803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5309) [M::mem_pestat] mean and std.dev: (1107.37, 1471.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7062) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1161.062 CPU sec, 51.285 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 642971, 67, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (565, 1130, 1430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3160) [M::mem_pestat] mean and std.dev: (944.85, 482.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4025) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.15, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 140, 1383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4097) [M::mem_pestat] mean and std.dev: (568.90, 924.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5454) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1164.175 CPU sec, 58.912 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 644249, 45, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.00, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 51, 635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1853) [M::mem_pestat] mean and std.dev: (192.82, 382.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2462) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1162.379 CPU sec, 66.021 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 643359, 48, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (461, 1015, 2889) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7745) [M::mem_pestat] mean and std.dev: (1562.00, 1557.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10173) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.01, 97.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 44, 1809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5375) [M::mem_pestat] mean and std.dev: (930.18, 1497.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7158) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (819, 1116, 1295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2247) [M::mem_pestat] mean and std.dev: (926.56, 310.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2723) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1166.364 CPU sec, 51.602 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 642321, 47, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (330, 1273, 1737) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4551) [M::mem_pestat] mean and std.dev: (1016.10, 661.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5958) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.07, 97.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 43, 2781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8309) [M::mem_pestat] mean and std.dev: (1160.39, 1734.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11073) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1177.349 CPU sec, 69.043 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 642968, 49, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.13, 97.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 76, 1924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5714) [M::mem_pestat] mean and std.dev: (884.15, 1322.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7609) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1161.857 CPU sec, 51.556 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 642704, 39, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (385, 529, 1159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2707) [M::mem_pestat] mean and std.dev: (718.71, 434.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.05, 97.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 62, 2059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6131) [M::mem_pestat] mean and std.dev: (950.11, 1580.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8167) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (849, 878, 1271) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (5, 2115) [M::mem_pestat] mean and std.dev: (850.67, 233.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2537) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1170.617 CPU sec, 67.539 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 644143, 47, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (336, 1173, 2129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5715) [M::mem_pestat] mean and std.dev: (1001.42, 734.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.89, 97.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 31, 230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 656) [M::mem_pestat] mean and std.dev: (50.62, 87.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1155.538 CPU sec, 51.143 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 642776, 41, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (356, 466, 1491) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3761) [M::mem_pestat] mean and std.dev: (667.36, 625.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.01, 97.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 78, 635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1855) [M::mem_pestat] mean and std.dev: (154.62, 291.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2465) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1174.824 CPU sec, 68.504 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643669, 31, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (352, 462, 857) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1867) [M::mem_pestat] mean and std.dev: (653.00, 424.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2372) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.08, 97.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 34, 507) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1499) [M::mem_pestat] mean and std.dev: (120.80, 291.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1995) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1037, 2219, 4584) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11678) [M::mem_pestat] mean and std.dev: (2618.92, 2184.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15225) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1161.311 CPU sec, 51.531 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 643451, 48, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.01, 97.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 143, 1900) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5632) [M::mem_pestat] mean and std.dev: (825.43, 1317.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7498) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1169.758 CPU sec, 63.326 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 643000, 51, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (375, 502, 1395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3435) [M::mem_pestat] mean and std.dev: (716.87, 649.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4455) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.94, 96.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 144, 1895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5625) [M::mem_pestat] mean and std.dev: (976.84, 1370.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7490) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1165.173 CPU sec, 67.985 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 643432, 49, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.28, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 71, 1159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3447) [M::mem_pestat] mean and std.dev: (388.68, 771.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4591) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1158.394 CPU sec, 51.382 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643224, 41, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (367, 561, 922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2032) [M::mem_pestat] mean and std.dev: (685.70, 448.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2587) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.87, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 44, 2873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8581) [M::mem_pestat] mean and std.dev: (1021.44, 1768.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11435) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1169.624 CPU sec, 68.352 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 643289, 44, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.13, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 142, 2865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8547) [M::mem_pestat] mean and std.dev: (1213.79, 1655.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11388) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1156.202 CPU sec, 51.200 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643281, 46, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (701, 1524, 2662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6584) [M::mem_pestat] mean and std.dev: (1552.50, 997.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8545) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.16, 97.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 54, 606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1780) [M::mem_pestat] mean and std.dev: (166.05, 345.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2367) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (302, 855, 1486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3854) [M::mem_pestat] mean and std.dev: (953.70, 668.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5038) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1167.907 CPU sec, 67.667 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 643343, 47, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 372, 1162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2998) [M::mem_pestat] mean and std.dev: (742.17, 728.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3916) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.06, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 57, 1900) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5654) [M::mem_pestat] mean and std.dev: (654.61, 1163.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7531) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1162.676 CPU sec, 51.417 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 643371, 50, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (395, 773, 1439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3527) [M::mem_pestat] mean and std.dev: (1034.25, 853.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.07, 97.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 64, 1317) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3915) [M::mem_pestat] mean and std.dev: (400.77, 817.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5214) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1287, 1870, 3409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7653) [M::mem_pestat] mean and std.dev: (2528.82, 1730.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9775) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1172.683 CPU sec, 67.687 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643495, 44, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (453, 615, 1185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2649) [M::mem_pestat] mean and std.dev: (701.27, 405.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3381) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.11, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 101, 1899) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5667) [M::mem_pestat] mean and std.dev: (633.76, 1065.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7551) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1158.208 CPU sec, 51.290 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643546, 47, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.02, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 69, 1795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5345) [M::mem_pestat] mean and std.dev: (839.27, 1346.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7120) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1167.797 CPU sec, 65.420 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643147, 37, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (356, 664, 992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2264) [M::mem_pestat] mean and std.dev: (552.80, 277.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2900) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.93, 97.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 71, 1535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4545) [M::mem_pestat] mean and std.dev: (750.92, 1296.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6050) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1166.432 CPU sec, 66.429 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643581, 45, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (773, 1098, 1782) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3800) [M::mem_pestat] mean and std.dev: (1276.36, 748.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.18, 97.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 49, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 670) [M::mem_pestat] mean and std.dev: (69.33, 112.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 886) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1175, 1806, 2566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5348) [M::mem_pestat] mean and std.dev: (1614.10, 874.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6739) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1166.088 CPU sec, 51.943 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643509, 42, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.73, 97.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 55, 644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1902) [M::mem_pestat] mean and std.dev: (201.53, 431.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2531) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1168.331 CPU sec, 67.129 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643553, 41, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (429, 1535, 1893) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4821) [M::mem_pestat] mean and std.dev: (1141.92, 702.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6285) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.04, 97.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 83, 2985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8897) [M::mem_pestat] mean and std.dev: (1474.98, 2185.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11853) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1154.943 CPU sec, 51.171 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 642987, 53, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.22, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 45, 2074) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6178) [M::mem_pestat] mean and std.dev: (983.80, 1581.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8230) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1167.401 CPU sec, 67.268 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 642924, 39, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (368, 589, 1142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2690) [M::mem_pestat] mean and std.dev: (759.92, 612.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3464) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.18, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 67, 1758) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5240) [M::mem_pestat] mean and std.dev: (675.41, 1138.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6981) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1152.551 CPU sec, 51.059 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643754, 53, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (445, 592, 1017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2161) [M::mem_pestat] mean and std.dev: (763.90, 469.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.86, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 125, 1427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4227) [M::mem_pestat] mean and std.dev: (450.47, 768.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5627) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1168.151 CPU sec, 68.064 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 642702, 45, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (298, 389, 771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1717) [M::mem_pestat] mean and std.dev: (488.44, 335.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2190) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.20, 97.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 92, 1795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5355) [M::mem_pestat] mean and std.dev: (986.20, 1363.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7135) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1162.024 CPU sec, 51.420 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 642993, 58, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (377, 530, 945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2081) [M::mem_pestat] mean and std.dev: (601.31, 344.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2649) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.95, 97.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 87, 671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1953) [M::mem_pestat] mean and std.dev: (168.74, 370.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2594) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1170.378 CPU sec, 68.620 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 642746, 47, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 484, 846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2110) [M::mem_pestat] mean and std.dev: (499.00, 359.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2742) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.91, 97.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 58, 1522) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4542) [M::mem_pestat] mean and std.dev: (667.28, 1097.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6052) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1171.203 CPU sec, 66.366 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 643199, 63, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (349, 612, 2080) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5542) [M::mem_pestat] mean and std.dev: (1274.38, 1334.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7273) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.99, 97.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 39, 2119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6315) [M::mem_pestat] mean and std.dev: (836.90, 1391.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8413) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1162.496 CPU sec, 51.597 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 642725, 47, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (321, 575, 1025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2433) [M::mem_pestat] mean and std.dev: (650.53, 472.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3137) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.13, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 64, 1565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4649) [M::mem_pestat] mean and std.dev: (744.43, 1357.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6191) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1172.985 CPU sec, 67.459 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643023, 38, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (297, 615, 2421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6669) [M::mem_pestat] mean and std.dev: (1351.08, 1559.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.99, 97.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 61, 1070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3178) [M::mem_pestat] mean and std.dev: (460.50, 838.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4232) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1156.769 CPU sec, 51.188 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 643546, 48, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (298, 449, 4148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11848) [M::mem_pestat] mean and std.dev: (1569.76, 1968.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.01, 97.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 54, 618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1818) [M::mem_pestat] mean and std.dev: (245.74, 437.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2418) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1165.682 CPU sec, 66.849 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643194, 42, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.95, 97.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 108, 692) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2026) [M::mem_pestat] mean and std.dev: (246.06, 448.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2693) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1160.408 CPU sec, 51.284 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643755, 41, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (394, 1048, 1387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3373) [M::mem_pestat] mean and std.dev: (1134.40, 785.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.97, 97.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 88, 1510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4474) [M::mem_pestat] mean and std.dev: (635.70, 1246.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5956) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1171.042 CPU sec, 68.748 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642831, 44, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (521, 715, 1606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3776) [M::mem_pestat] mean and std.dev: (938.64, 584.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4861) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.03, 96.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 159, 1546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4580) [M::mem_pestat] mean and std.dev: (757.02, 1095.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6097) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1169.572 CPU sec, 51.882 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643440, 53, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (404, 450, 1439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3509) [M::mem_pestat] mean and std.dev: (646.55, 505.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4544) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.99, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 60, 2969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8861) [M::mem_pestat] mean and std.dev: (1479.89, 2373.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11807) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1168.436 CPU sec, 68.265 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643226, 47, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (439, 628, 786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1480) [M::mem_pestat] mean and std.dev: (571.90, 251.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.86, 97.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 62, 2541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7589) [M::mem_pestat] mean and std.dev: (1127.43, 1636.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10113) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (758, 954, 1542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3110) [M::mem_pestat] mean and std.dev: (1082.33, 496.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3894) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1164.074 CPU sec, 51.693 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643213, 43, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.89, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 91, 843) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2487) [M::mem_pestat] mean and std.dev: (290.81, 537.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3309) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1172.921 CPU sec, 67.020 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642740, 41, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (793, 908, 1435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2719) [M::mem_pestat] mean and std.dev: (944.20, 502.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.04, 96.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 44, 1176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3502) [M::mem_pestat] mean and std.dev: (403.46, 766.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4665) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1156.349 CPU sec, 51.004 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643206, 43, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 381, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1531) [M::mem_pestat] mean and std.dev: (528.46, 374.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2025) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.04, 97.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 39, 596) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1762) [M::mem_pestat] mean and std.dev: (145.71, 325.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2345) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1167.823 CPU sec, 67.592 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643294, 45, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (401, 816, 1322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3164) [M::mem_pestat] mean and std.dev: (806.64, 653.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4085) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.79, 97.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 43, 669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1981) [M::mem_pestat] mean and std.dev: (157.78, 360.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2637) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1162.829 CPU sec, 51.560 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 642485, 42, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.00, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 63, 575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1683) [M::mem_pestat] mean and std.dev: (226.97, 350.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2237) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1171.190 CPU sec, 67.855 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643138, 43, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (280, 439, 961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2323) [M::mem_pestat] mean and std.dev: (573.00, 479.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.09, 97.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 32, 1919) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5717) [M::mem_pestat] mean and std.dev: (902.20, 1421.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7616) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1170.221 CPU sec, 51.886 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643292, 32, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.98, 97.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 42, 688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2038) [M::mem_pestat] mean and std.dev: (282.21, 533.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2713) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1173.143 CPU sec, 68.213 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643024, 42, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (363, 426, 1389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3441) [M::mem_pestat] mean and std.dev: (705.08, 608.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.11, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 54, 1888) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5628) [M::mem_pestat] mean and std.dev: (865.00, 1473.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7498) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1161.007 CPU sec, 51.349 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 642767, 37, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (456, 475, 1037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2199) [M::mem_pestat] mean and std.dev: (644.56, 357.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.94, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 62, 2075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6167) [M::mem_pestat] mean and std.dev: (812.37, 1460.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8213) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1168.559 CPU sec, 61.659 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643144, 50, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (396, 430, 583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (22, 957) [M::mem_pestat] mean and std.dev: (399.60, 146.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.02, 97.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 126, 1502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4446) [M::mem_pestat] mean and std.dev: (501.40, 879.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5918) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1168.538 CPU sec, 64.931 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 643401, 52, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (358, 691, 1075) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2509) [M::mem_pestat] mean and std.dev: (670.15, 458.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3226) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.09, 97.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 35, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 256) [M::mem_pestat] mean and std.dev: (39.00, 37.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (883, 1157, 3445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8569) [M::mem_pestat] mean and std.dev: (1751.18, 1450.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11131) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1160.964 CPU sec, 51.300 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643141, 48, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 504, 844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1908) [M::mem_pestat] mean and std.dev: (505.67, 246.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2440) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.01, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 52, 1167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3465) [M::mem_pestat] mean and std.dev: (342.00, 733.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4614) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1176.505 CPU sec, 69.303 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643251, 49, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 1035, 1463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3995) [M::mem_pestat] mean and std.dev: (816.55, 547.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5261) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.99, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 63, 2058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6132) [M::mem_pestat] mean and std.dev: (878.85, 1485.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8169) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1169.480 CPU sec, 51.845 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 642853, 33, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (301, 689, 1335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3403) [M::mem_pestat] mean and std.dev: (941.31, 793.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.85, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 50, 1787) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5323) [M::mem_pestat] mean and std.dev: (703.09, 1099.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7091) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (450, 725, 1652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4056) [M::mem_pestat] mean and std.dev: (1037.10, 946.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5258) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1169.201 CPU sec, 67.435 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643354, 43, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (383, 521, 1182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2780) [M::mem_pestat] mean and std.dev: (748.45, 534.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.02, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 131, 1688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5016) [M::mem_pestat] mean and std.dev: (674.59, 1148.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6680) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1162.615 CPU sec, 51.911 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643505, 42, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (482, 586, 1105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2351) [M::mem_pestat] mean and std.dev: (790.50, 524.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2974) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.14, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 47, 1061) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3129) [M::mem_pestat] mean and std.dev: (339.17, 699.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4163) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (415, 843, 1786) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4528) [M::mem_pestat] mean and std.dev: (1082.60, 820.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5899) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1167.216 CPU sec, 68.167 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643150, 37, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (406, 469, 818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1642) [M::mem_pestat] mean and std.dev: (513.80, 239.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2054) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.89, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 49, 2008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5988) [M::mem_pestat] mean and std.dev: (593.18, 1035.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7978) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1164.434 CPU sec, 51.694 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643039, 40, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (407, 598, 1614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4028) [M::mem_pestat] mean and std.dev: (967.83, 955.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5235) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.13, 96.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 38, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (40.00, 35.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1167.954 CPU sec, 64.715 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 643221, 68, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (343, 476, 1072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2530) [M::mem_pestat] mean and std.dev: (544.31, 493.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3259) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.94, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 53, 2999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8967) [M::mem_pestat] mean and std.dev: (1585.35, 2209.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11951) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1168.039 CPU sec, 67.588 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 642927, 37, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 705, 1081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2639) [M::mem_pestat] mean and std.dev: (594.42, 422.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3418) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.05, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 49, 1451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4311) [M::mem_pestat] mean and std.dev: (455.56, 944.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5741) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (448, 874, 1446) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3442) [M::mem_pestat] mean and std.dev: (1016.36, 775.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4440) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1163.442 CPU sec, 51.569 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 642486, 50, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 488, 1224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2996) [M::mem_pestat] mean and std.dev: (706.19, 471.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3882) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.07, 97.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 95, 1469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4363) [M::mem_pestat] mean and std.dev: (659.43, 1186.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5810) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1180.060 CPU sec, 68.193 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 642881, 39, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.09, 97.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 139, 1408) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4170) [M::mem_pestat] mean and std.dev: (623.22, 907.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5551) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1161.001 CPU sec, 51.409 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 642770, 51, 14) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.06, 97.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 171, 2844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8480) [M::mem_pestat] mean and std.dev: (1416.00, 2071.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11298) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (627, 1441, 2249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5493) [M::mem_pestat] mean and std.dev: (1527.79, 1020.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7115) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1169.743 CPU sec, 66.774 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 642508, 45, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.84, 96.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 39, 1757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5235) [M::mem_pestat] mean and std.dev: (444.18, 922.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6974) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1160.777 CPU sec, 51.448 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643321, 36, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.95, 97.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 40, 2910) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8686) [M::mem_pestat] mean and std.dev: (1276.49, 2317.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11574) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (832, 1137, 1380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2476) [M::mem_pestat] mean and std.dev: (1121.80, 471.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3024) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1169.176 CPU sec, 67.980 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 642390, 29, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.87, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 58, 1767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5243) [M::mem_pestat] mean and std.dev: (691.41, 1243.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6981) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1162.726 CPU sec, 51.437 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 642484, 48, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (451, 975, 1370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3208) [M::mem_pestat] mean and std.dev: (911.77, 462.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4127) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.84, 96.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 43, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (63.05, 107.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1093) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1176.426 CPU sec, 68.409 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643333, 41, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (221, 292, 929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2345) [M::mem_pestat] mean and std.dev: (462.36, 367.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3053) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.98, 97.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 132, 2702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8036) [M::mem_pestat] mean and std.dev: (1061.97, 1615.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10703) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (581, 1163, 1751) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4091) [M::mem_pestat] mean and std.dev: (914.75, 448.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5261) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1163.748 CPU sec, 67.705 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 643377, 57, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (373, 672, 2022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5320) [M::mem_pestat] mean and std.dev: (1138.76, 1075.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6969) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.01, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 50, 773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2269) [M::mem_pestat] mean and std.dev: (141.98, 292.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3017) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (775, 1248, 1717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3601) [M::mem_pestat] mean and std.dev: (1057.11, 594.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4543) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1161.602 CPU sec, 51.645 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 642856, 39, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (465, 752, 1321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3033) [M::mem_pestat] mean and std.dev: (895.54, 628.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3889) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.00, 97.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 59, 2902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8642) [M::mem_pestat] mean and std.dev: (1435.31, 2225.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1008, 1130, 5647) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14925) [M::mem_pestat] mean and std.dev: (2352.00, 2228.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 19564) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1174.336 CPU sec, 67.584 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643146, 35, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (339, 1113, 1397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3513) [M::mem_pestat] mean and std.dev: (920.78, 736.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4571) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.91, 97.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 88, 1748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5172) [M::mem_pestat] mean and std.dev: (906.94, 1408.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6884) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1159.642 CPU sec, 51.409 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 643369, 44, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.94, 97.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 50, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (57.53, 64.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (586, 1768, 2299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5725) [M::mem_pestat] mean and std.dev: (1377.56, 793.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7438) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1170.727 CPU sec, 68.387 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643153, 43, 18) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.02, 97.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 58, 626) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1840) [M::mem_pestat] mean and std.dev: (259.62, 479.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2447) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (477, 1190, 2536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6654) [M::mem_pestat] mean and std.dev: (1713.61, 1678.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8713) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1171.543 CPU sec, 52.140 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643506, 36, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.98, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 60, 1593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4737) [M::mem_pestat] mean and std.dev: (593.68, 1122.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6309) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1167.514 CPU sec, 68.778 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 642810, 46, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.94, 97.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 49, 1633) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4857) [M::mem_pestat] mean and std.dev: (700.31, 1329.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6469) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1160.794 CPU sec, 51.440 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643828, 51, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (438, 547, 1781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4467) [M::mem_pestat] mean and std.dev: (914.50, 673.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5810) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.81, 97.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 92, 1113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3299) [M::mem_pestat] mean and std.dev: (404.86, 769.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4392) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1176.262 CPU sec, 68.483 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643175, 53, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (385, 771, 1241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2953) [M::mem_pestat] mean and std.dev: (728.33, 431.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3809) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.02, 97.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 34, 398) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1164) [M::mem_pestat] mean and std.dev: (82.60, 170.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1547) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1160.441 CPU sec, 51.299 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 643108, 43, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (519, 1395, 2438) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6276) [M::mem_pestat] mean and std.dev: (1608.76, 1405.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8195) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.11, 97.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 37, 1817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5423) [M::mem_pestat] mean and std.dev: (811.78, 1466.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7226) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1172.612 CPU sec, 67.803 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 642584, 45, 15) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (328, 540, 1173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2863) [M::mem_pestat] mean and std.dev: (603.75, 435.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3708) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.17, 97.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 126, 3033) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9069) [M::mem_pestat] mean and std.dev: (1372.52, 2160.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12087) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (802, 1012, 1237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2107) [M::mem_pestat] mean and std.dev: (1038.13, 438.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2791) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1162.888 CPU sec, 51.533 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 642908, 39, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (384, 901, 2133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5631) [M::mem_pestat] mean and std.dev: (940.00, 693.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7380) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.99, 97.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 197, 2418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7218) [M::mem_pestat] mean and std.dev: (1277.03, 1969.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9618) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1169.928 CPU sec, 66.482 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643328, 55, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (304, 422, 1170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2902) [M::mem_pestat] mean and std.dev: (609.45, 452.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3768) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.87, 97.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 84, 1641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4881) [M::mem_pestat] mean and std.dev: (726.73, 1203.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6501) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (845, 998, 1653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3269) [M::mem_pestat] mean and std.dev: (1033.00, 515.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4077) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1158.725 CPU sec, 51.459 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643577, 35, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (452, 1077, 3264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8888) [M::mem_pestat] mean and std.dev: (1792.55, 2099.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11700) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.82, 96.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 134, 1111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3287) [M::mem_pestat] mean and std.dev: (379.93, 603.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4375) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1163.199 CPU sec, 67.106 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643116, 53, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 420, 3237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9133) [M::mem_pestat] mean and std.dev: (1700.50, 2158.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12081) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.97, 97.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 101, 1192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3526) [M::mem_pestat] mean and std.dev: (520.86, 877.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4693) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1156.768 CPU sec, 51.034 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643637, 46, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (576, 764, 1242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2574) [M::mem_pestat] mean and std.dev: (875.00, 550.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3240) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.87, 97.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 58, 1913) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5711) [M::mem_pestat] mean and std.dev: (866.18, 1420.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7610) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1161.787 CPU sec, 66.620 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643135, 61, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.05, 97.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 106, 1449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4287) [M::mem_pestat] mean and std.dev: (601.96, 1017.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5706) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (627, 961, 1911) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4479) [M::mem_pestat] mean and std.dev: (1040.33, 710.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5763) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1158.283 CPU sec, 51.394 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 642888, 45, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (455, 767, 1486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3548) [M::mem_pestat] mean and std.dev: (1007.08, 846.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4579) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.09, 97.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 130, 1948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5800) [M::mem_pestat] mean and std.dev: (762.54, 1272.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7726) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1155.171 CPU sec, 58.615 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 642855, 46, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (378, 843, 1246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2982) [M::mem_pestat] mean and std.dev: (869.69, 650.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3850) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.14, 97.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 47, 1840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5482) [M::mem_pestat] mean and std.dev: (793.83, 1313.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7303) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1169.639 CPU sec, 67.402 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643729, 47, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 758) [M::mem_pestat] mean and std.dev: (442.40, 97.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 42, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1363) [M::mem_pestat] mean and std.dev: (145.26, 269.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1812) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (515, 878, 1592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3746) [M::mem_pestat] mean and std.dev: (807.56, 465.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4823) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1160.198 CPU sec, 51.250 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 643416, 46, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (866, 1311, 1589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3035) [M::mem_pestat] mean and std.dev: (1041.33, 456.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.89, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 44, 838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2470) [M::mem_pestat] mean and std.dev: (305.30, 605.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3286) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1170.385 CPU sec, 67.316 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643183, 43, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (619, 690, 2177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5293) [M::mem_pestat] mean and std.dev: (864.00, 589.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6851) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.06, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 75, 1895) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5639) [M::mem_pestat] mean and std.dev: (774.30, 1210.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7511) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1165.008 CPU sec, 51.740 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643197, 40, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.97, 96.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 141, 1957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5821) [M::mem_pestat] mean and std.dev: (996.47, 1581.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7753) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1133, 1264, 2411) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4967) [M::mem_pestat] mean and std.dev: (1540.30, 1045.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6245) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1160.710 CPU sec, 68.029 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 643269, 39, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 398, 1087) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2603) [M::mem_pestat] mean and std.dev: (538.50, 379.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3361) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.13, 97.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 38, 1989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5929) [M::mem_pestat] mean and std.dev: (918.76, 1581.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7899) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1159.787 CPU sec, 51.469 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 643877, 37, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.90, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 72, 926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2706) [M::mem_pestat] mean and std.dev: (304.62, 581.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3596) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1162.487 CPU sec, 67.915 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 643021, 54, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (365, 499, 1293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3149) [M::mem_pestat] mean and std.dev: (781.41, 643.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4077) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.85, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 45, 1996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5948) [M::mem_pestat] mean and std.dev: (686.35, 1147.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7924) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1160.246 CPU sec, 51.648 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 642630, 51, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.06, 97.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 65, 1396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4148) [M::mem_pestat] mean and std.dev: (550.40, 902.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5524) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (342, 1105, 1456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3684) [M::mem_pestat] mean and std.dev: (1068.40, 701.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4798) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1165.598 CPU sec, 64.416 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643598, 47, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.22, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 42, 1717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5111) [M::mem_pestat] mean and std.dev: (739.72, 1320.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6808) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1162.902 CPU sec, 64.552 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643175, 35, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (349, 653, 1336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3310) [M::mem_pestat] mean and std.dev: (733.78, 609.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4297) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.92, 97.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 44, 1156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3416) [M::mem_pestat] mean and std.dev: (498.24, 825.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4546) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1165.052 CPU sec, 51.533 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 643533, 51, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.96, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 48, 1104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3290) [M::mem_pestat] mean and std.dev: (407.54, 804.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4383) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1159.087 CPU sec, 66.898 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 643233, 39, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (344, 623, 1533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3911) [M::mem_pestat] mean and std.dev: (983.40, 897.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5100) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.91, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 113, 1365) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4049) [M::mem_pestat] mean and std.dev: (597.95, 1005.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5391) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1163.730 CPU sec, 51.625 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643147, 49, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (347, 1002, 3581) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10049) [M::mem_pestat] mean and std.dev: (2038.40, 2255.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13283) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.08, 97.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 52, 1887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5635) [M::mem_pestat] mean and std.dev: (785.74, 1312.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7509) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1162.976 CPU sec, 67.123 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643477, 43, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (276, 381, 904) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2160) [M::mem_pestat] mean and std.dev: (461.58, 316.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2788) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.04, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 90, 1195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3525) [M::mem_pestat] mean and std.dev: (376.79, 642.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4690) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1159.757 CPU sec, 51.554 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 642741, 52, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (393, 812, 2191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5787) [M::mem_pestat] mean and std.dev: (1264.17, 1402.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7585) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.77, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 62, 1268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3762) [M::mem_pestat] mean and std.dev: (503.29, 966.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5009) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1169.425 CPU sec, 68.252 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 642990, 52, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.09, 97.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 154, 1606) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4778) [M::mem_pestat] mean and std.dev: (695.56, 1178.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6364) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1162.595 CPU sec, 51.524 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643026, 42, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (181, 380, 1463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4027) [M::mem_pestat] mean and std.dev: (692.23, 685.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5309) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.04, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 82, 983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2911) [M::mem_pestat] mean and std.dev: (385.35, 684.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3875) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1165.417 CPU sec, 65.920 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 642618, 43, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (360, 486, 810) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1710) [M::mem_pestat] mean and std.dev: (490.00, 286.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2160) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (124, 754) [M::mem_pestat] mean and std.dev: (441.60, 97.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 880) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 65, 1076) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3178) [M::mem_pestat] mean and std.dev: (336.53, 656.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4229) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (781, 1520, 1999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4435) [M::mem_pestat] mean and std.dev: (1399.67, 767.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5653) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1167.710 CPU sec, 68.039 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643112, 48, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (404, 871, 1691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4265) [M::mem_pestat] mean and std.dev: (1240.21, 1051.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5552) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.76, 97.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 40, 226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 638) [M::mem_pestat] mean and std.dev: (60.95, 92.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 844) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1161.598 CPU sec, 51.592 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 643328, 51, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.10, 97.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 46, 1814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5392) [M::mem_pestat] mean and std.dev: (904.90, 1579.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7223) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1164.498 CPU sec, 67.087 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 643099, 38, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (293, 389, 1855) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4979) [M::mem_pestat] mean and std.dev: (908.50, 887.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6541) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.11, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 210, 1586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4716) [M::mem_pestat] mean and std.dev: (973.39, 1422.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6664) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1159.642 CPU sec, 51.515 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 643174, 38, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.87, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 104, 3242) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9664) [M::mem_pestat] mean and std.dev: (1598.61, 2232.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12875) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (634, 909, 1963) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4621) [M::mem_pestat] mean and std.dev: (1264.09, 995.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5950) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1165.002 CPU sec, 67.779 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643111, 34, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.14, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 55, 1546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4604) [M::mem_pestat] mean and std.dev: (762.45, 1272.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6133) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1159.068 CPU sec, 51.445 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643536, 41, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (371, 532, 1631) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4151) [M::mem_pestat] mean and std.dev: (804.85, 580.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.08, 97.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 61, 1842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5496) [M::mem_pestat] mean and std.dev: (891.02, 1481.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7323) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (762, 1373, 1795) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3861) [M::mem_pestat] mean and std.dev: (1285.70, 632.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4894) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1161.250 CPU sec, 67.587 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643372, 36, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.09, 96.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 55, 1866) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5544) [M::mem_pestat] mean and std.dev: (913.51, 1426.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7383) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1159.503 CPU sec, 51.297 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643328, 49, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 395, 1045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2551) [M::mem_pestat] mean and std.dev: (612.67, 454.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3304) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.87, 97.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 41, 4054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12118) [M::mem_pestat] mean and std.dev: (1717.65, 2723.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16150) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1169.027 CPU sec, 68.655 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 642953, 37, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.95, 97.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 142, 1305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3863) [M::mem_pestat] mean and std.dev: (538.21, 804.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5142) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1169.711 CPU sec, 68.456 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 642833, 58, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (368, 433, 1078) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2498) [M::mem_pestat] mean and std.dev: (730.00, 514.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3208) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.21, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 50, 2400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7150) [M::mem_pestat] mean and std.dev: (943.98, 1555.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9525) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (953, 1407, 1803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3503) [M::mem_pestat] mean and std.dev: (1410.10, 539.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4353) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1164.811 CPU sec, 51.734 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643084, 46, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.27, 97.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 238, 3015) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9003) [M::mem_pestat] mean and std.dev: (1699.54, 2308.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11997) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1173.900 CPU sec, 68.429 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643247, 45, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (229, 466, 586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1300) [M::mem_pestat] mean and std.dev: (382.92, 192.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.92, 97.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 88, 1853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5503) [M::mem_pestat] mean and std.dev: (919.43, 1327.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7328) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1157.954 CPU sec, 51.230 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643225, 49, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (549, 745, 1634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3804) [M::mem_pestat] mean and std.dev: (868.90, 529.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4889) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.04, 97.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 37, 148) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (43.77, 47.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 550) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1166.665 CPU sec, 67.518 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643278, 49, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (389, 555, 1010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2252) [M::mem_pestat] mean and std.dev: (690.54, 419.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2873) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.02, 97.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 73, 1703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5051) [M::mem_pestat] mean and std.dev: (671.59, 1098.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6725) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (285, 1000, 1211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3063) [M::mem_pestat] mean and std.dev: (848.21, 539.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3989) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1156.062 CPU sec, 51.106 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 643265, 50, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.19, 97.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 1042, 3034) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9032) [M::mem_pestat] mean and std.dev: (1986.35, 2720.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12870) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1168.053 CPU sec, 69.062 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 642719, 47, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (362, 600, 1535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3881) [M::mem_pestat] mean and std.dev: (875.06, 557.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5054) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.95, 97.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (41, 128, 1239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3635) [M::mem_pestat] mean and std.dev: (486.36, 752.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4833) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1199, 1643, 2733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5801) [M::mem_pestat] mean and std.dev: (1430.44, 636.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7335) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1161.992 CPU sec, 51.495 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643251, 47, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 581, 1258) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3120) [M::mem_pestat] mean and std.dev: (819.23, 512.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4051) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.11, 97.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 120, 2337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6957) [M::mem_pestat] mean and std.dev: (1052.24, 1578.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9267) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1168.429 CPU sec, 68.776 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643123, 40, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (278, 688, 930) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2234) [M::mem_pestat] mean and std.dev: (627.67, 447.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2886) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.15, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 46, 2141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6381) [M::mem_pestat] mean and std.dev: (1024.05, 1597.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8501) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (736, 1609, 1876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4156) [M::mem_pestat] mean and std.dev: (1348.33, 803.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5296) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1158.061 CPU sec, 51.257 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 643338, 43, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (382, 423, 1397) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3427) [M::mem_pestat] mean and std.dev: (803.80, 594.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4442) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.02, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 83, 1930) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5738) [M::mem_pestat] mean and std.dev: (851.03, 1437.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7642) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1168.657 CPU sec, 67.454 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643588, 41, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.95, 97.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 99, 2423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7209) [M::mem_pestat] mean and std.dev: (1173.49, 1719.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9602) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1039, 1459, 1694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3004) [M::mem_pestat] mean and std.dev: (1376.90, 665.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4040) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1153.086 CPU sec, 51.325 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643227, 40, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (381, 754, 1116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2586) [M::mem_pestat] mean and std.dev: (802.09, 592.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.16, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 80, 902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2652) [M::mem_pestat] mean and std.dev: (286.74, 551.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3527) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (503, 1138, 2248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5738) [M::mem_pestat] mean and std.dev: (1306.08, 942.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7483) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1168.505 CPU sec, 67.860 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 642536, 59, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.06, 97.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 53, 1588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4720) [M::mem_pestat] mean and std.dev: (705.98, 1227.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6286) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1163.981 CPU sec, 51.665 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 643287, 47, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (368, 406, 1013) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2303) [M::mem_pestat] mean and std.dev: (640.93, 485.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.14, 97.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 52, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1601) [M::mem_pestat] mean and std.dev: (93.08, 212.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2130) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1173.885 CPU sec, 67.142 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 642837, 45, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.94, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 52, 2713) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8079) [M::mem_pestat] mean and std.dev: (851.49, 1538.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10762) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1160.516 CPU sec, 51.398 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643706, 40, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.18, 97.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 44, 413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1193) [M::mem_pestat] mean and std.dev: (82.88, 127.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1583) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1166.650 CPU sec, 68.379 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643242, 41, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (405, 503, 1240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2910) [M::mem_pestat] mean and std.dev: (614.27, 419.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3745) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.07, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 90, 3059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9129) [M::mem_pestat] mean and std.dev: (1410.25, 2069.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12164) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1152.379 CPU sec, 50.994 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642918, 32, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (358, 785, 1948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5128) [M::mem_pestat] mean and std.dev: (1087.45, 904.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6718) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.27, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 56, 2621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7831) [M::mem_pestat] mean and std.dev: (1308.39, 2031.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10436) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1162.360 CPU sec, 61.299 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 642960, 45, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 741, 1098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2684) [M::mem_pestat] mean and std.dev: (754.89, 575.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.78, 97.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 52, 1654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4906) [M::mem_pestat] mean and std.dev: (471.69, 1044.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6532) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1163.528 CPU sec, 68.607 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643295, 47, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (303, 460, 1401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3597) [M::mem_pestat] mean and std.dev: (859.30, 746.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4695) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.98, 97.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 60, 1873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5579) [M::mem_pestat] mean and std.dev: (1019.11, 1643.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7591) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1153.513 CPU sec, 51.182 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643144, 47, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.03, 97.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 210, 3277) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9785) [M::mem_pestat] mean and std.dev: (1816.28, 2584.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13039) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1171.015 CPU sec, 67.339 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643890, 51, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (342, 495, 916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2064) [M::mem_pestat] mean and std.dev: (479.50, 244.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2638) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.98, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 112, 4409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13187) [M::mem_pestat] mean and std.dev: (2226.33, 3047.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17576) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1157.231 CPU sec, 51.310 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643280, 46, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (616, 963, 1718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3922) [M::mem_pestat] mean and std.dev: (995.30, 553.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5024) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.17, 97.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 126, 2410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7180) [M::mem_pestat] mean and std.dev: (1045.66, 1555.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9565) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (327, 616, 890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2016) [M::mem_pestat] mean and std.dev: (682.36, 394.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2579) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1172.215 CPU sec, 68.544 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643493, 34, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (450, 695, 1049) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2247) [M::mem_pestat] mean and std.dev: (719.30, 504.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2846) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.03, 97.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 91, 688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2022) [M::mem_pestat] mean and std.dev: (167.85, 369.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2689) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1158.499 CPU sec, 51.289 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643715, 54, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (461, 613, 1303) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2987) [M::mem_pestat] mean and std.dev: (873.71, 598.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3829) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.18, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 39, 2297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6849) [M::mem_pestat] mean and std.dev: (875.22, 1520.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9125) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1166.435 CPU sec, 67.764 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 642996, 32, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (391, 1035, 1583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3967) [M::mem_pestat] mean and std.dev: (1160.50, 964.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5159) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.01, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 68, 4129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12317) [M::mem_pestat] mean and std.dev: (1663.91, 2530.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16411) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (414, 1352, 1990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5142) [M::mem_pestat] mean and std.dev: (1223.80, 762.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6718) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1158.464 CPU sec, 51.472 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643148, 42, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.89, 96.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 146, 1954) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5820) [M::mem_pestat] mean and std.dev: (1143.55, 1558.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7753) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1162.720 CPU sec, 65.406 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 642321, 36, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (210, 395, 1925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5355) [M::mem_pestat] mean and std.dev: (929.08, 813.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7070) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.11, 97.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (46, 148, 4396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13096) [M::mem_pestat] mean and std.dev: (2058.61, 2970.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17446) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (486, 1120, 1381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3171) [M::mem_pestat] mean and std.dev: (974.50, 453.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4066) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1167.951 CPU sec, 68.081 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643335, 44, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (435, 668, 778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1464) [M::mem_pestat] mean and std.dev: (540.75, 204.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1807) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.92, 97.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 210, 2628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7850) [M::mem_pestat] mean and std.dev: (1156.12, 1598.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10461) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1158.514 CPU sec, 51.407 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643212, 37, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 392, 1973) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5295) [M::mem_pestat] mean and std.dev: (1028.85, 1060.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6956) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.09, 97.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 67, 2834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8480) [M::mem_pestat] mean and std.dev: (712.03, 1322.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11303) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (305, 635, 1517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3941) [M::mem_pestat] mean and std.dev: (733.22, 535.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5153) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1163.320 CPU sec, 67.451 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 642826, 44, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (449, 606, 1430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3392) [M::mem_pestat] mean and std.dev: (1010.53, 870.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4494) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.16, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 340, 2648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7882) [M::mem_pestat] mean and std.dev: (1258.32, 1757.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10499) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (614, 1209, 2809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7199) [M::mem_pestat] mean and std.dev: (1682.46, 1348.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9394) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1159.983 CPU sec, 51.580 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643895, 53, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (641, 1350, 2947) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7559) [M::mem_pestat] mean and std.dev: (1497.00, 1134.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9865) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.14, 97.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 44, 846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2486) [M::mem_pestat] mean and std.dev: (202.30, 422.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3306) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1170.080 CPU sec, 68.286 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643371, 56, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (276, 368, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 915) [M::mem_pestat] mean and std.dev: (348.56, 126.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1128) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.04, 97.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 95, 4029) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 12053) [M::mem_pestat] mean and std.dev: (1865.66, 2834.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 16065) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (687, 897, 1367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2727) [M::mem_pestat] mean and std.dev: (799.67, 351.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3407) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1152.899 CPU sec, 51.027 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643175, 44, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (434, 577, 1197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2723) [M::mem_pestat] mean and std.dev: (689.00, 398.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.90, 97.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 95, 2010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5988) [M::mem_pestat] mean and std.dev: (810.77, 1355.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7977) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (842, 1247, 2103) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4625) [M::mem_pestat] mean and std.dev: (1345.62, 831.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5886) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1170.387 CPU sec, 67.883 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643252, 45, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (462, 696, 1194) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2658) [M::mem_pestat] mean and std.dev: (780.42, 575.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.89, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 38, 1709) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5091) [M::mem_pestat] mean and std.dev: (807.80, 1336.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6782) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1161.806 CPU sec, 51.404 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 643192, 46, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.87, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 59, 742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2184) [M::mem_pestat] mean and std.dev: (156.58, 260.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2905) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1165.234 CPU sec, 68.484 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643683, 39, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 1024, 2362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6598) [M::mem_pestat] mean and std.dev: (1270.54, 1140.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8716) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.14, 97.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 67, 2407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7155) [M::mem_pestat] mean and std.dev: (962.76, 1574.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9529) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1162.272 CPU sec, 67.536 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 642998, 54, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (336, 445, 2006) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5346) [M::mem_pestat] mean and std.dev: (993.14, 1168.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7016) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.96, 97.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 41, 948) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2818) [M::mem_pestat] mean and std.dev: (317.39, 635.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3753) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1157.250 CPU sec, 51.339 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643285, 51, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.08, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 49, 705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2079) [M::mem_pestat] mean and std.dev: (272.11, 503.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2766) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1168.322 CPU sec, 68.972 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643051, 46, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.00, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 47, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (79.51, 113.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1163.492 CPU sec, 51.382 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643092, 42, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (383, 1421, 2319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6191) [M::mem_pestat] mean and std.dev: (1373.90, 1165.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8127) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.94, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 76, 928) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2744) [M::mem_pestat] mean and std.dev: (350.38, 633.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3652) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1163.599 CPU sec, 67.736 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643202, 46, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (464, 724, 1426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3350) [M::mem_pestat] mean and std.dev: (853.31, 504.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4312) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.11, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 64) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 162) [M::mem_pestat] mean and std.dev: (27.44, 23.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 211) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1165.143 CPU sec, 51.706 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643281, 48, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (399, 467, 1844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4734) [M::mem_pestat] mean and std.dev: (957.73, 823.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6179) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.78, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 97, 2536) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7566) [M::mem_pestat] mean and std.dev: (1017.57, 1589.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10081) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1166.557 CPU sec, 67.701 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642973, 42, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (335, 523, 1339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3347) [M::mem_pestat] mean and std.dev: (769.27, 520.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4351) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.15, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (6, 39, 897) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2679) [M::mem_pestat] mean and std.dev: (265.08, 546.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3570) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1161.986 CPU sec, 51.421 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 642586, 50, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (373, 575, 1293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3133) [M::mem_pestat] mean and std.dev: (818.36, 590.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4053) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.94, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 181, 3014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8984) [M::mem_pestat] mean and std.dev: (1441.55, 2250.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11969) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (786, 1022, 2514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5970) [M::mem_pestat] mean and std.dev: (1362.36, 1014.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7698) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1171.855 CPU sec, 69.030 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 642926, 55, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (382, 456, 752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1492) [M::mem_pestat] mean and std.dev: (499.44, 202.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1862) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.25, 97.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 53, 621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1823) [M::mem_pestat] mean and std.dev: (120.98, 225.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2424) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1155.796 CPU sec, 51.354 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642972, 50, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (582, 767, 1327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2817) [M::mem_pestat] mean and std.dev: (835.91, 448.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.96, 97.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 39, 143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 409) [M::mem_pestat] mean and std.dev: (39.41, 49.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 542) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1165.846 CPU sec, 68.358 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 642780, 48, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.05, 97.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 59, 1329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3947) [M::mem_pestat] mean and std.dev: (363.90, 690.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5256) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1156.984 CPU sec, 51.143 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643301, 36, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (319, 440, 937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2173) [M::mem_pestat] mean and std.dev: (621.43, 469.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.16, 97.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 125, 2068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6146) [M::mem_pestat] mean and std.dev: (1214.25, 1622.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8185) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1164.184 CPU sec, 66.419 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642528, 44, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (780, 1005, 2098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4734) [M::mem_pestat] mean and std.dev: (1159.10, 805.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6052) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.03, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 51, 1514) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4494) [M::mem_pestat] mean and std.dev: (637.79, 1151.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5984) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1156.867 CPU sec, 51.339 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 643330, 41, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.12, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 35, 605) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1779) [M::mem_pestat] mean and std.dev: (187.11, 356.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2366) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1166.371 CPU sec, 67.229 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 642972, 48, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (321, 403, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1245) [M::mem_pestat] mean and std.dev: (472.40, 316.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.97, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 54, 1128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3324) [M::mem_pestat] mean and std.dev: (330.86, 573.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4422) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1157.138 CPU sec, 51.348 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643087, 31, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (232, 440, 802) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1942) [M::mem_pestat] mean and std.dev: (363.67, 211.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.20, 97.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 131, 1618) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4790) [M::mem_pestat] mean and std.dev: (908.80, 1381.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6434) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1160.627 CPU sec, 67.591 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 642606, 35, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.02, 97.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 73, 1515) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4487) [M::mem_pestat] mean and std.dev: (604.59, 982.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5973) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1156.835 CPU sec, 51.313 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643279, 45, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (385, 677, 1245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2965) [M::mem_pestat] mean and std.dev: (827.64, 531.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3825) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.03, 97.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 94, 2707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8091) [M::mem_pestat] mean and std.dev: (1233.51, 1720.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10783) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1149.685 CPU sec, 60.532 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643928, 42, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (215, 394, 1050) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2720) [M::mem_pestat] mean and std.dev: (580.83, 506.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3555) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.82, 97.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 96, 290) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 850) [M::mem_pestat] mean and std.dev: (122.94, 180.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1130) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1157.747 CPU sec, 64.749 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 643247, 50, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 758) [M::mem_pestat] mean and std.dev: (442.39, 97.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 885) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 108, 1568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4658) [M::mem_pestat] mean and std.dev: (681.82, 1275.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6203) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1159.521 CPU sec, 51.364 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643298, 43, 16) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (399, 571, 1024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2274) [M::mem_pestat] mean and std.dev: (641.30, 339.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2899) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.07, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 47, 1183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3515) [M::mem_pestat] mean and std.dev: (363.62, 630.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4681) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (731, 1205, 2664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6530) [M::mem_pestat] mean and std.dev: (1664.50, 1538.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8463) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1158.639 CPU sec, 66.500 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643100, 44, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (487, 1408, 1720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4186) [M::mem_pestat] mean and std.dev: (1120.54, 721.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5419) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.96, 97.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 43, 1490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4428) [M::mem_pestat] mean and std.dev: (633.20, 1191.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5897) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1031, 1501, 2955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6803) [M::mem_pestat] mean and std.dev: (1994.70, 1399.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8727) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1152.344 CPU sec, 50.929 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 643535, 43, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (382, 538, 1127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2617) [M::mem_pestat] mean and std.dev: (654.27, 423.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3362) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.13, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 46, 635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1861) [M::mem_pestat] mean and std.dev: (111.94, 273.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2474) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1164.707 CPU sec, 67.764 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643653, 40, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.28, 97.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 48, 1206) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3574) [M::mem_pestat] mean and std.dev: (499.11, 971.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4758) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1150.475 CPU sec, 50.922 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643130, 41, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 770, 1193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3073) [M::mem_pestat] mean and std.dev: (815.31, 631.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4013) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.08, 97.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 96, 1863) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5555) [M::mem_pestat] mean and std.dev: (948.27, 1480.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7401) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (387, 645, 1099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2523) [M::mem_pestat] mean and std.dev: (643.08, 414.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3235) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1164.986 CPU sec, 67.828 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 642827, 53, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 298, 818) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1982) [M::mem_pestat] mean and std.dev: (579.47, 517.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.10, 97.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 107, 1726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5122) [M::mem_pestat] mean and std.dev: (802.32, 1336.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6820) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1158.579 CPU sec, 51.513 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 642874, 47, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.07, 97.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 45, 1533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4563) [M::mem_pestat] mean and std.dev: (885.53, 1476.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6793) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1155.713 CPU sec, 63.014 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643305, 53, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 387, 1346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3484) [M::mem_pestat] mean and std.dev: (687.25, 510.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.01, 97.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 39, 1386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4122) [M::mem_pestat] mean and std.dev: (446.91, 912.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5490) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1169.408 CPU sec, 66.236 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 642529, 41, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 473, 1897) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5093) [M::mem_pestat] mean and std.dev: (606.09, 502.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6691) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.04, 97.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 430, 1944) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5766) [M::mem_pestat] mean and std.dev: (944.23, 1338.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7677) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1162.244 CPU sec, 51.585 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 642933, 48, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (328, 478, 1374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3466) [M::mem_pestat] mean and std.dev: (729.58, 558.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4512) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.82, 97.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 68, 1974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5870) [M::mem_pestat] mean and std.dev: (909.18, 1613.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7818) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1159.940 CPU sec, 66.653 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643158, 41, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (401, 541, 3667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10199) [M::mem_pestat] mean and std.dev: (1482.46, 1919.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13465) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.82, 97.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 148, 4391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13127) [M::mem_pestat] mean and std.dev: (1972.49, 2857.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17495) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (780, 838, 1279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2277) [M::mem_pestat] mean and std.dev: (837.11, 340.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2776) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1155.897 CPU sec, 51.130 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 643580, 34, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.93, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 61, 2418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7222) [M::mem_pestat] mean and std.dev: (1083.21, 1719.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9624) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1143.925 CPU sec, 65.506 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643048, 54, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (683, 915, 1107) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1955) [M::mem_pestat] mean and std.dev: (772.90, 310.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2379) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.02, 97.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 58, 1579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4709) [M::mem_pestat] mean and std.dev: (756.47, 1262.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6274) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1156.390 CPU sec, 51.317 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643490, 46, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 351, 1282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3268) [M::mem_pestat] mean and std.dev: (664.38, 521.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4261) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.92, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 61, 2010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5976) [M::mem_pestat] mean and std.dev: (897.67, 1437.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7959) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (415, 1060, 1747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4411) [M::mem_pestat] mean and std.dev: (1129.10, 711.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5743) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1150.709 CPU sec, 66.242 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 642959, 61, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.04, 97.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 49, 730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2158) [M::mem_pestat] mean and std.dev: (212.65, 444.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2872) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1153.101 CPU sec, 51.033 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 642866, 53, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.88, 97.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 163, 2451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7293) [M::mem_pestat] mean and std.dev: (1307.61, 1930.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9714) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1162.444 CPU sec, 67.497 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643184, 50, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 1139, 2060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5596) [M::mem_pestat] mean and std.dev: (923.17, 742.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7364) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.08, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 743, 2933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8731) [M::mem_pestat] mean and std.dev: (1774.50, 2166.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11630) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (255, 1001, 1123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2859) [M::mem_pestat] mean and std.dev: (1006.00, 684.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3745) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1169.552 CPU sec, 69.170 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 643630, 46, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (377, 648, 1479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3683) [M::mem_pestat] mean and std.dev: (851.74, 679.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4785) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.98, 97.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 41, 459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1339) [M::mem_pestat] mean and std.dev: (108.68, 200.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1779) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1162.178 CPU sec, 51.474 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 643171, 53, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.98, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 61, 435) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1265) [M::mem_pestat] mean and std.dev: (81.21, 107.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1680) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1157.499 CPU sec, 66.646 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643281, 52, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (268, 668, 959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2341) [M::mem_pestat] mean and std.dev: (651.50, 424.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3032) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.02, 97.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 53, 1008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2990) [M::mem_pestat] mean and std.dev: (301.54, 590.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3981) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1146.021 CPU sec, 50.780 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643370, 34, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.97, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 42, 1363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4065) [M::mem_pestat] mean and std.dev: (407.83, 735.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5416) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1153.620 CPU sec, 66.938 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 642849, 50, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.92, 97.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 56, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 723) [M::mem_pestat] mean and std.dev: (87.59, 115.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 957) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1153.972 CPU sec, 51.191 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643254, 49, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (569, 721, 2110) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5192) [M::mem_pestat] mean and std.dev: (1299.40, 1256.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6733) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.16, 97.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 100, 1546) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4584) [M::mem_pestat] mean and std.dev: (803.61, 1419.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6480) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1163.330 CPU sec, 67.872 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642426, 50, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (320, 436, 926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2138) [M::mem_pestat] mean and std.dev: (468.10, 254.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.01, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 166, 2159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6431) [M::mem_pestat] mean and std.dev: (1042.92, 1372.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8567) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (814, 1091, 1304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2284) [M::mem_pestat] mean and std.dev: (977.18, 254.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2774) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1162.943 CPU sec, 51.486 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643279, 43, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (395, 557, 1275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3035) [M::mem_pestat] mean and std.dev: (764.60, 666.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3915) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.00, 97.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 49, 302) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 860) [M::mem_pestat] mean and std.dev: (88.23, 120.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1139) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1158.203 CPU sec, 67.215 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643358, 48, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (374, 469, 1975) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5177) [M::mem_pestat] mean and std.dev: (923.23, 758.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.82, 97.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 111, 2579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7701) [M::mem_pestat] mean and std.dev: (1105.75, 1591.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10262) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1149.536 CPU sec, 51.032 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642572, 41, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (342, 382, 798) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1710) [M::mem_pestat] mean and std.dev: (506.90, 397.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2166) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.92, 97.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 67, 2066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6142) [M::mem_pestat] mean and std.dev: (704.53, 1273.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8180) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1164.658 CPU sec, 67.616 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643225, 40, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.96, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 106, 3492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10404) [M::mem_pestat] mean and std.dev: (1524.22, 2365.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13860) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (951, 1755, 2864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6690) [M::mem_pestat] mean and std.dev: (2063.22, 1659.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8700) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1153.073 CPU sec, 51.278 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643333, 45, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (469, 615, 1682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4108) [M::mem_pestat] mean and std.dev: (873.70, 598.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.13, 97.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 82, 2272) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6760) [M::mem_pestat] mean and std.dev: (1295.37, 1943.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9071) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1155.355 CPU sec, 65.122 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643322, 45, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.90, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 33, 291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 837) [M::mem_pestat] mean and std.dev: (48.91, 59.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1110) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1148.142 CPU sec, 50.689 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 643506, 54, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (527, 780, 1865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4541) [M::mem_pestat] mean and std.dev: (936.53, 659.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5879) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.21, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 124, 2653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7911) [M::mem_pestat] mean and std.dev: (1342.02, 1950.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10540) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1148.899 CPU sec, 64.730 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643519, 48, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.90, 97.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 41, 2690) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8040) [M::mem_pestat] mean and std.dev: (1188.59, 2008.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10715) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1144.594 CPU sec, 50.642 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643191, 41, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 395, 649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1297) [M::mem_pestat] mean and std.dev: (376.45, 191.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1621) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.05, 97.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 131, 2226) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6614) [M::mem_pestat] mean and std.dev: (1196.47, 1800.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8808) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1146.252 CPU sec, 63.917 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643085, 42, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.03, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 63, 1216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3628) [M::mem_pestat] mean and std.dev: (358.50, 629.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4834) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1151.516 CPU sec, 51.117 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 643258, 49, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.02, 97.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 50, 1186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3514) [M::mem_pestat] mean and std.dev: (334.88, 607.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4678) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1160.099 CPU sec, 58.158 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 642767, 38, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.00, 97.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 68, 1337) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3965) [M::mem_pestat] mean and std.dev: (506.76, 857.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5279) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1167.122 CPU sec, 63.207 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643142, 39, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (447, 700, 1048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2250) [M::mem_pestat] mean and std.dev: (650.08, 314.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2851) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.98, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 52, 2634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7866) [M::mem_pestat] mean and std.dev: (1147.92, 1766.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10482) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1157.764 CPU sec, 51.256 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643515, 46, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 779, 1098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2636) [M::mem_pestat] mean and std.dev: (764.14, 459.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3405) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.18, 97.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 62, 293) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 845) [M::mem_pestat] mean and std.dev: (60.00, 68.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1121) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1150.963 CPU sec, 64.341 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643457, 46, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 570, 1327) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3327) [M::mem_pestat] mean and std.dev: (886.60, 820.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.81, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 110, 998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2930) [M::mem_pestat] mean and std.dev: (410.10, 754.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3896) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1146.897 CPU sec, 50.919 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 642873, 65, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 540, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1484) [M::mem_pestat] mean and std.dev: (469.92, 275.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1902) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.03, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 56, 1458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4326) [M::mem_pestat] mean and std.dev: (509.79, 966.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5760) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1144.903 CPU sec, 65.531 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643619, 41, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (410, 450, 990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2150) [M::mem_pestat] mean and std.dev: (735.82, 592.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3105) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.83, 97.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 148, 3814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11388) [M::mem_pestat] mean and std.dev: (1647.78, 2718.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15175) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1150.546 CPU sec, 50.963 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 642974, 53, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.99, 97.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 55, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (58.73, 65.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1157.718 CPU sec, 64.707 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643300, 44, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (486, 1175, 2316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5976) [M::mem_pestat] mean and std.dev: (1403.42, 1190.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7806) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.12, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 38, 1808) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5400) [M::mem_pestat] mean and std.dev: (565.27, 1095.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7196) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (792, 1710, 2248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5160) [M::mem_pestat] mean and std.dev: (1258.30, 600.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6616) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1146.562 CPU sec, 50.605 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 643523, 57, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (380, 435, 3762) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10526) [M::mem_pestat] mean and std.dev: (1526.88, 1709.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13908) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.11, 97.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 1764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5262) [M::mem_pestat] mean and std.dev: (980.23, 1517.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7050) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1164.321 CPU sec, 63.515 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 642534, 52, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (335, 376, 1370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3440) [M::mem_pestat] mean and std.dev: (759.15, 581.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4475) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.01, 97.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 97, 3205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9547) [M::mem_pestat] mean and std.dev: (1745.44, 2735.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12718) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1174.636 CPU sec, 67.104 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 643332, 38, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.86, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 41, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 297) [M::mem_pestat] mean and std.dev: (46.00, 41.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 388) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1155.299 CPU sec, 51.208 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642813, 41, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (457, 1045, 1412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3322) [M::mem_pestat] mean and std.dev: (919.90, 654.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4277) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.99, 97.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 32, 150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 418) [M::mem_pestat] mean and std.dev: (44.29, 52.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1169.254 CPU sec, 68.109 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643910, 66, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (391, 455, 1035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2323) [M::mem_pestat] mean and std.dev: (700.30, 557.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2967) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.99, 97.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 61, 1573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4685) [M::mem_pestat] mean and std.dev: (817.16, 1371.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6301) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1154.013 CPU sec, 51.427 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643671, 41, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 329, 999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2537) [M::mem_pestat] mean and std.dev: (482.25, 373.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3306) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.99, 97.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 41, 1990) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5938) [M::mem_pestat] mean and std.dev: (816.26, 1473.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7912) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1166.422 CPU sec, 67.337 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642846, 44, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (533, 812, 1842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4460) [M::mem_pestat] mean and std.dev: (1203.50, 1020.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5769) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.75, 96.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 58, 1855) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5529) [M::mem_pestat] mean and std.dev: (885.20, 1492.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7366) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1164.907 CPU sec, 51.521 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 643109, 48, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (336, 747, 2470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6738) [M::mem_pestat] mean and std.dev: (1223.69, 1270.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8872) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.02, 97.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 45, 1402) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4172) [M::mem_pestat] mean and std.dev: (560.47, 960.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5557) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1164.142 CPU sec, 68.030 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643473, 51, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.00, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 44, 1116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3316) [M::mem_pestat] mean and std.dev: (303.36, 556.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4416) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1153.582 CPU sec, 51.237 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 642598, 49, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (493, 571, 1010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2044) [M::mem_pestat] mean and std.dev: (698.93, 373.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2561) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.00, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 203, 2139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6361) [M::mem_pestat] mean and std.dev: (1022.85, 1334.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8472) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1164.258 CPU sec, 66.162 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 642861, 46, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.05, 97.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 66, 1209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3587) [M::mem_pestat] mean and std.dev: (539.31, 864.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4776) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (506, 891, 1264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2780) [M::mem_pestat] mean and std.dev: (958.09, 616.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3538) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1169.247 CPU sec, 69.135 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 642756, 43, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (812, 1234, 2140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4796) [M::mem_pestat] mean and std.dev: (1109.40, 705.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6124) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.99, 97.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 93, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1456) [M::mem_pestat] mean and std.dev: (127.00, 163.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1933) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1159.949 CPU sec, 51.387 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 643393, 46, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.96, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 41, 1497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4463) [M::mem_pestat] mean and std.dev: (665.50, 1345.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6049) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1166.572 CPU sec, 70.060 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 642578, 39, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (589, 855, 1965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4717) [M::mem_pestat] mean and std.dev: (1139.35, 1074.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6093) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.14, 97.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 104, 1881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5583) [M::mem_pestat] mean and std.dev: (968.59, 1481.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7434) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1156.032 CPU sec, 51.103 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 642962, 41, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (396, 1009, 1595) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3993) [M::mem_pestat] mean and std.dev: (881.67, 575.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5192) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.85, 97.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 41, 1625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4843) [M::mem_pestat] mean and std.dev: (772.80, 1329.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6452) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1156.997 CPU sec, 67.311 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 642645, 41, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (600, 996, 1707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3921) [M::mem_pestat] mean and std.dev: (1143.21, 809.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5028) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.99, 97.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 139, 2592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7710) [M::mem_pestat] mean and std.dev: (1177.88, 1819.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10269) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1162.280 CPU sec, 51.479 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643519, 54, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (360, 451, 933) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2079) [M::mem_pestat] mean and std.dev: (567.33, 389.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2652) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.84, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 133, 513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1483) [M::mem_pestat] mean and std.dev: (188.64, 289.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1968) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1152.005 CPU sec, 66.584 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643016, 41, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (156, 563, 956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2556) [M::mem_pestat] mean and std.dev: (501.56, 370.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3356) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.13, 97.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 47, 2176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6488) [M::mem_pestat] mean and std.dev: (838.54, 1410.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8644) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1157.034 CPU sec, 51.295 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643190, 32, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (286, 409, 715) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1573) [M::mem_pestat] mean and std.dev: (459.40, 203.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2002) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.19, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 385, 3032) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9008) [M::mem_pestat] mean and std.dev: (1684.59, 2081.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11996) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1154.152 CPU sec, 68.035 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643119, 51, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.08, 97.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 122, 1865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5547) [M::mem_pestat] mean and std.dev: (792.25, 1241.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7388) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1162.655 CPU sec, 69.697 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 643504, 32, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (309, 612, 1149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2829) [M::mem_pestat] mean and std.dev: (837.20, 700.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3669) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.89, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 41, 1188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3512) [M::mem_pestat] mean and std.dev: (272.11, 601.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4674) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1157.277 CPU sec, 51.213 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 643243, 43, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (461, 692, 1158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2552) [M::mem_pestat] mean and std.dev: (842.76, 550.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3249) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.10, 97.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 153, 1908) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5680) [M::mem_pestat] mean and std.dev: (943.10, 1349.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7566) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1153.837 CPU sec, 65.468 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 643065, 35, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.17, 97.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 48, 237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 673) [M::mem_pestat] mean and std.dev: (79.17, 111.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 891) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1147.702 CPU sec, 50.714 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 642688, 42, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.02, 97.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 312, 2600) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7756) [M::mem_pestat] mean and std.dev: (1187.10, 1735.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10334) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1145.814 CPU sec, 65.824 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643151, 51, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (295, 1374, 1492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3886) [M::mem_pestat] mean and std.dev: (904.78, 590.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5083) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.04, 97.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 64, 597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1727) [M::mem_pestat] mean and std.dev: (237.24, 396.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2292) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1142.788 CPU sec, 50.489 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643271, 48, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 542, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1550) [M::mem_pestat] mean and std.dev: (535.60, 361.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1980) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.04, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 92, 2195) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6527) [M::mem_pestat] mean and std.dev: (906.50, 1473.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8693) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1145.268 CPU sec, 66.266 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 643616, 45, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (308, 487, 1437) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3695) [M::mem_pestat] mean and std.dev: (651.71, 528.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.03, 97.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 62, 1916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5700) [M::mem_pestat] mean and std.dev: (645.24, 1139.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7592) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (976, 1134, 1781) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3391) [M::mem_pestat] mean and std.dev: (926.00, 521.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4196) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1150.077 CPU sec, 50.995 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 643316, 41, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.00, 96.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 43, 1992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5924) [M::mem_pestat] mean and std.dev: (712.03, 1123.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7890) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1150.472 CPU sec, 67.296 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 642969, 46, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.15, 97.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 51, 1855) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5531) [M::mem_pestat] mean and std.dev: (861.26, 1376.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7369) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1146.663 CPU sec, 50.812 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643191, 54, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 726, 1429) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3619) [M::mem_pestat] mean and std.dev: (863.69, 684.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4714) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.93, 97.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 126, 1072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3158) [M::mem_pestat] mean and std.dev: (391.28, 641.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4201) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1440, 1501, 1664) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (992, 2112) [M::mem_pestat] mean and std.dev: (1520.67, 79.39) [M::mem_pestat] low and high boundaries for proper pairs: (768, 2336) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1154.196 CPU sec, 66.750 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643257, 52, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (392, 540, 1363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3305) [M::mem_pestat] mean and std.dev: (845.00, 658.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4276) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.88, 97.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 30, 800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2366) [M::mem_pestat] mean and std.dev: (144.88, 432.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3149) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (943, 1332, 1846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3652) [M::mem_pestat] mean and std.dev: (1181.78, 591.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4555) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1150.832 CPU sec, 51.011 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 642920, 46, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (393, 531, 1164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2706) [M::mem_pestat] mean and std.dev: (707.11, 596.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.05, 97.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 110, 2236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6684) [M::mem_pestat] mean and std.dev: (1200.32, 1697.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8908) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1144.550 CPU sec, 66.291 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643567, 53, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (402, 1353, 1769) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4503) [M::mem_pestat] mean and std.dev: (1251.46, 834.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5870) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.86, 96.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 58, 1348) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3992) [M::mem_pestat] mean and std.dev: (455.91, 828.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5314) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1143.171 CPU sec, 50.572 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 642602, 39, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.12, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 51, 1256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3720) [M::mem_pestat] mean and std.dev: (354.12, 741.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4952) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1155.137 CPU sec, 66.784 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643874, 47, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (358, 539, 1562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3970) [M::mem_pestat] mean and std.dev: (649.30, 503.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5174) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.97, 97.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 113, 1259) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3725) [M::mem_pestat] mean and std.dev: (445.85, 782.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4958) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (852, 1208, 2182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4842) [M::mem_pestat] mean and std.dev: (1367.80, 941.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6172) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1147.648 CPU sec, 50.689 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 642868, 55, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.02, 97.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 51, 1826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5444) [M::mem_pestat] mean and std.dev: (966.33, 1460.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7253) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1151.603 CPU sec, 67.115 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643205, 44, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (443, 996, 1156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2582) [M::mem_pestat] mean and std.dev: (772.36, 443.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.02, 97.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 82, 1470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4350) [M::mem_pestat] mean and std.dev: (571.36, 986.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5790) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (797, 1969, 2145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4841) [M::mem_pestat] mean and std.dev: (1532.22, 1037.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6189) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1157.169 CPU sec, 51.273 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 642854, 42, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (346, 682, 1817) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4759) [M::mem_pestat] mean and std.dev: (1022.08, 970.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6230) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.03, 97.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 73, 1529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4533) [M::mem_pestat] mean and std.dev: (618.90, 1098.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6035) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1143.061 CPU sec, 60.272 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643167, 39, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 554, 3061) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8515) [M::mem_pestat] mean and std.dev: (1545.46, 2053.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11242) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.12, 96.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 132, 1551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4583) [M::mem_pestat] mean and std.dev: (850.45, 1345.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6233) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1153.806 CPU sec, 62.984 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 642927, 46, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.09, 97.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 52, 453) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1321) [M::mem_pestat] mean and std.dev: (104.86, 187.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1755) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1153.781 CPU sec, 50.979 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 642933, 45, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.99, 97.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 107, 3185) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9505) [M::mem_pestat] mean and std.dev: (1312.44, 2022.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12665) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1145.508 CPU sec, 65.232 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 643143, 44, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.13, 97.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 75, 987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2911) [M::mem_pestat] mean and std.dev: (390.49, 780.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3873) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1146.067 CPU sec, 50.734 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 642962, 41, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (363, 721, 1674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4296) [M::mem_pestat] mean and std.dev: (614.25, 432.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5607) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.96, 96.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 42, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1244) [M::mem_pestat] mean and std.dev: (76.42, 130.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1653) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1150.280 CPU sec, 66.161 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643445, 36, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.09, 97.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (44, 128, 2529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7499) [M::mem_pestat] mean and std.dev: (1130.50, 1503.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9984) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1154.967 CPU sec, 51.323 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643311, 45, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.91, 97.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 64, 860) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2512) [M::mem_pestat] mean and std.dev: (163.81, 419.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3338) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1151.998 CPU sec, 67.801 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643201, 49, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.09, 97.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 52, 466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1350) [M::mem_pestat] mean and std.dev: (153.74, 264.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1792) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (668, 924, 2236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5372) [M::mem_pestat] mean and std.dev: (1706.46, 1403.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7320) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1150.253 CPU sec, 51.083 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 642772, 31, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (226, 330, 884) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2200) [M::mem_pestat] mean and std.dev: (368.83, 254.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2858) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.97, 97.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 115, 1149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3377) [M::mem_pestat] mean and std.dev: (406.14, 630.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4491) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1151.264 CPU sec, 64.486 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 642956, 57, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (303, 428, 1696) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4482) [M::mem_pestat] mean and std.dev: (524.36, 427.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5875) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.00, 96.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 109, 2299) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6851) [M::mem_pestat] mean and std.dev: (1139.38, 1786.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9127) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (811, 1059, 1466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2776) [M::mem_pestat] mean and std.dev: (1173.00, 623.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3666) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1158.580 CPU sec, 66.714 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643367, 41, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.03, 97.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 50, 2090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6230) [M::mem_pestat] mean and std.dev: (874.46, 1591.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8300) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1149.997 CPU sec, 50.804 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643086, 40, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.80, 97.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 39, 1517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4527) [M::mem_pestat] mean and std.dev: (632.67, 1126.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6032) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1151.507 CPU sec, 67.876 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643035, 40, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.08, 97.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 85, 1998) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5962) [M::mem_pestat] mean and std.dev: (1019.62, 1657.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7944) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1145.309 CPU sec, 50.634 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 643517, 37, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (461, 667, 1965) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4973) [M::mem_pestat] mean and std.dev: (1199.00, 969.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6477) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.94, 97.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 70, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1677) [M::mem_pestat] mean and std.dev: (153.77, 298.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2232) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1146.053 CPU sec, 65.440 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643609, 44, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (349, 460, 1241) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3025) [M::mem_pestat] mean and std.dev: (1007.00, 922.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4698) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.09, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 41, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (76.09, 132.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1203) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1139.452 CPU sec, 50.404 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 642832, 47, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (419, 593, 1321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3125) [M::mem_pestat] mean and std.dev: (755.58, 459.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4027) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.10, 97.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 69, 906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2662) [M::mem_pestat] mean and std.dev: (380.84, 646.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3540) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1155.402 CPU sec, 65.899 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 643285, 40, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (388, 709, 2528) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6808) [M::mem_pestat] mean and std.dev: (1392.38, 1497.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8948) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.11, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 77, 2354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6984) [M::mem_pestat] mean and std.dev: (935.14, 1529.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9299) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1150.842 CPU sec, 50.967 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643252, 40, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.12, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 42, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (59.84, 83.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1208) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1558, 1740, 2295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (84, 3769) [M::mem_pestat] mean and std.dev: (1852.18, 964.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5710) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1155.588 CPU sec, 67.694 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 643177, 39, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (423, 553, 1197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2745) [M::mem_pestat] mean and std.dev: (635.21, 329.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3519) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.04, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 74, 1451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4309) [M::mem_pestat] mean and std.dev: (524.67, 939.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5738) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1166.476 CPU sec, 67.597 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643564, 38, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (389, 606, 1693) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4301) [M::mem_pestat] mean and std.dev: (1090.46, 1018.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5605) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.03, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (37, 607, 4024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11998) [M::mem_pestat] mean and std.dev: (2196.92, 2822.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15985) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1159.020 CPU sec, 51.450 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643265, 33, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (547, 1244, 1578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3640) [M::mem_pestat] mean and std.dev: (1035.31, 641.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.04, 97.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 195, 2671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7971) [M::mem_pestat] mean and std.dev: (1323.16, 1776.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10621) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1147.917 CPU sec, 66.117 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 643158, 52, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.95, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 61, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2169) [M::mem_pestat] mean and std.dev: (257.36, 471.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2887) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (863, 1241, 2723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6443) [M::mem_pestat] mean and std.dev: (1493.92, 886.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8303) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1150.296 CPU sec, 51.063 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643296, 40, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (363, 419, 874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1896) [M::mem_pestat] mean and std.dev: (432.44, 196.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2407) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.93, 97.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 53, 3218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9612) [M::mem_pestat] mean and std.dev: (1616.97, 2437.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12809) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1151.446 CPU sec, 66.885 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 643530, 38, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.08, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 47, 307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (93.42, 175.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1174) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1147.427 CPU sec, 50.854 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 643311, 49, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (285, 372, 1702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4536) [M::mem_pestat] mean and std.dev: (834.87, 853.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5953) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.09, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 83, 2214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6594) [M::mem_pestat] mean and std.dev: (1057.20, 1563.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8784) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (829, 1035, 1360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2422) [M::mem_pestat] mean and std.dev: (996.10, 299.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2953) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1152.906 CPU sec, 67.095 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 643180, 52, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.96, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 42, 249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 703) [M::mem_pestat] mean and std.dev: (68.81, 113.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 930) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1151.559 CPU sec, 51.017 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 643111, 43, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.10, 97.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 79, 3278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9780) [M::mem_pestat] mean and std.dev: (1667.33, 2611.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13031) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1152.835 CPU sec, 67.802 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 642934, 44, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.09, 96.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 137, 1579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4691) [M::mem_pestat] mean and std.dev: (808.60, 1226.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6247) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1153.539 CPU sec, 51.007 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 643571, 38, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.93, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 42, 1578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4714) [M::mem_pestat] mean and std.dev: (801.00, 1349.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6282) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (463, 542, 1359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3151) [M::mem_pestat] mean and std.dev: (700.20, 448.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4047) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1154.374 CPU sec, 67.957 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 642986, 40, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (449, 726, 1609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3929) [M::mem_pestat] mean and std.dev: (735.64, 421.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5089) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.16, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 133, 2125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6313) [M::mem_pestat] mean and std.dev: (955.41, 1490.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8407) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1152.839 CPU sec, 51.279 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643219, 44, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (323, 534, 783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1703) [M::mem_pestat] mean and std.dev: (558.78, 334.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2163) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.07, 97.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 57, 1223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3635) [M::mem_pestat] mean and std.dev: (376.21, 792.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4841) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1153.434 CPU sec, 66.871 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 643713, 47, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (432, 596, 1203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2745) [M::mem_pestat] mean and std.dev: (716.21, 349.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3516) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.99, 97.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 39, 346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1006) [M::mem_pestat] mean and std.dev: (69.51, 112.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1336) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (702, 1339, 2809) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7023) [M::mem_pestat] mean and std.dev: (1755.50, 1375.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9130) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1144.596 CPU sec, 50.621 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 642879, 44, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.87, 97.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 47, 1247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3707) [M::mem_pestat] mean and std.dev: (412.41, 759.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4937) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (737, 1063, 1853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4085) [M::mem_pestat] mean and std.dev: (1032.55, 726.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5201) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1148.621 CPU sec, 64.867 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643377, 42, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 312, 1047) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2631) [M::mem_pestat] mean and std.dev: (680.40, 684.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3423) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.10, 97.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 70, 1387) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4101) [M::mem_pestat] mean and std.dev: (513.56, 858.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5458) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1152.341 CPU sec, 50.876 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643097, 47, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (390, 644, 1224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2892) [M::mem_pestat] mean and std.dev: (827.36, 543.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3726) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.97, 97.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 135, 1557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4623) [M::mem_pestat] mean and std.dev: (827.95, 1293.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6156) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1147.348 CPU sec, 65.037 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 643453, 55, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 587, 1264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3292) [M::mem_pestat] mean and std.dev: (767.94, 744.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4306) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.04, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 72, 2436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7266) [M::mem_pestat] mean and std.dev: (870.50, 1339.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9681) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1143.282 CPU sec, 50.804 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643326, 49, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.13, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 175, 2217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6579) [M::mem_pestat] mean and std.dev: (691.50, 991.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8760) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1142.581 CPU sec, 57.524 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642672, 44, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 359, 791) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1811) [M::mem_pestat] mean and std.dev: (452.90, 327.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2321) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.99, 97.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 107, 2675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7975) [M::mem_pestat] mean and std.dev: (1223.30, 1769.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10625) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1151.376 CPU sec, 65.037 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643124, 49, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (347, 1015, 1945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5141) [M::mem_pestat] mean and std.dev: (1150.07, 899.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.17, 96.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 51, 956) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2830) [M::mem_pestat] mean and std.dev: (292.05, 536.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3767) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (597, 1022, 1674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3828) [M::mem_pestat] mean and std.dev: (1266.10, 928.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4980) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1152.759 CPU sec, 50.985 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642455, 38, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (1291, 1406, 1767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (339, 2719) [M::mem_pestat] mean and std.dev: (1179.60, 560.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3422) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.99, 97.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 130, 1724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5124) [M::mem_pestat] mean and std.dev: (671.65, 1168.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6824) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1147.116 CPU sec, 65.857 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643056, 38, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (455, 693, 1282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2936) [M::mem_pestat] mean and std.dev: (584.12, 336.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3763) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.13, 97.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 54, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1891) [M::mem_pestat] mean and std.dev: (277.80, 486.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2517) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1143.705 CPU sec, 50.635 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 643048, 50, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.97, 97.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 43, 192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (69.95, 100.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 690) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1149.116 CPU sec, 66.349 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643926, 41, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 422, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1854) [M::mem_pestat] mean and std.dev: (470.08, 242.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2384) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.06, 97.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 87, 1712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5090) [M::mem_pestat] mean and std.dev: (901.95, 1587.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7253) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1144.414 CPU sec, 50.635 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 642592, 44, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.25, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 47, 671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1979) [M::mem_pestat] mean and std.dev: (118.97, 217.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2633) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1144.097 CPU sec, 65.959 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643458, 49, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (280, 417, 2719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7597) [M::mem_pestat] mean and std.dev: (885.75, 942.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10036) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.11, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 113, 1571) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4653) [M::mem_pestat] mean and std.dev: (688.22, 1044.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6194) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1145.364 CPU sec, 50.714 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 642827, 53, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 368, 1662) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4560) [M::mem_pestat] mean and std.dev: (631.00, 652.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6009) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.16, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 38, 1381) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4111) [M::mem_pestat] mean and std.dev: (635.79, 954.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5476) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (754, 1158, 1926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4270) [M::mem_pestat] mean and std.dev: (1160.91, 668.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5442) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1149.686 CPU sec, 62.731 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 643445, 47, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (497, 651, 1459) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3383) [M::mem_pestat] mean and std.dev: (737.54, 435.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4345) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.89, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 118, 3493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10439) [M::mem_pestat] mean and std.dev: (1533.43, 2254.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13912) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1151.185 CPU sec, 67.142 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 643206, 48, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (268, 698, 1702) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4570) [M::mem_pestat] mean and std.dev: (772.44, 612.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6004) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.03, 97.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 262, 1952) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5810) [M::mem_pestat] mean and std.dev: (678.65, 1056.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7739) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1149.813 CPU sec, 50.921 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643269, 42, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 1312, 1562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4156) [M::mem_pestat] mean and std.dev: (889.22, 611.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5453) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.82, 97.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 82, 2001) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5965) [M::mem_pestat] mean and std.dev: (936.54, 1408.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7947) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (538, 878, 985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1879) [M::mem_pestat] mean and std.dev: (725.67, 286.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2326) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1147.823 CPU sec, 66.487 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643125, 40, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (755, 1261, 6128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 16874) [M::mem_pestat] mean and std.dev: (3123.50, 3421.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 22247) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.08, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 54, 351) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 997) [M::mem_pestat] mean and std.dev: (109.12, 168.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1320) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (717, 872, 1602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3372) [M::mem_pestat] mean and std.dev: (1096.90, 739.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4257) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1150.055 CPU sec, 50.866 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 643359, 47, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.84, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 51, 755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2203) [M::mem_pestat] mean and std.dev: (253.17, 470.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2927) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1151.154 CPU sec, 66.931 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 642677, 56, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.94, 97.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 78, 1896) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5630) [M::mem_pestat] mean and std.dev: (866.68, 1498.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7497) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1153.090 CPU sec, 50.980 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643290, 38, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.26, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 56, 1330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3972) [M::mem_pestat] mean and std.dev: (653.94, 1045.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5293) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1140.641 CPU sec, 64.805 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 642896, 46, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.90, 97.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 78, 1910) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5690) [M::mem_pestat] mean and std.dev: (1005.07, 1661.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7652) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1151.207 CPU sec, 50.929 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643240, 52, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (147, 383, 1139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3123) [M::mem_pestat] mean and std.dev: (443.73, 412.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4115) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.90, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 94, 2423) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7217) [M::mem_pestat] mean and std.dev: (1112.41, 1473.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9614) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1150.906 CPU sec, 65.244 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 643466, 30, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (385, 602, 1292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3106) [M::mem_pestat] mean and std.dev: (831.41, 734.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4013) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.96, 97.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 56, 1949) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5811) [M::mem_pestat] mean and std.dev: (927.76, 1464.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7742) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1169.569 CPU sec, 67.868 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643276, 47, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (709, 1368, 2153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5041) [M::mem_pestat] mean and std.dev: (1299.10, 968.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6485) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.12, 97.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 47, 1431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4267) [M::mem_pestat] mean and std.dev: (570.14, 1008.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5685) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1155.442 CPU sec, 51.176 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643536, 36, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (339, 699, 1497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3813) [M::mem_pestat] mean and std.dev: (1003.00, 799.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4971) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.93, 97.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 135, 2939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8745) [M::mem_pestat] mean and std.dev: (1516.22, 2302.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11648) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (716, 1134, 3371) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8681) [M::mem_pestat] mean and std.dev: (1823.31, 1511.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11336) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1150.726 CPU sec, 66.956 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643517, 50, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 499, 676) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1352) [M::mem_pestat] mean and std.dev: (507.15, 241.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1690) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.88, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 41, 2354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7030) [M::mem_pestat] mean and std.dev: (1207.22, 1862.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9368) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (777, 893, 937) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (457, 1257) [M::mem_pestat] mean and std.dev: (840.75, 166.14) [M::mem_pestat] low and high boundaries for proper pairs: (176, 1505) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1157.391 CPU sec, 51.342 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642754, 39, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (542, 940, 1483) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3365) [M::mem_pestat] mean and std.dev: (813.10, 506.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4306) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.98, 97.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 70, 1699) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5033) [M::mem_pestat] mean and std.dev: (681.72, 1279.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6700) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1157.657 CPU sec, 67.706 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643073, 41, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (641, 986, 1859) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4295) [M::mem_pestat] mean and std.dev: (1293.31, 925.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5513) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.03, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 57, 946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2778) [M::mem_pestat] mean and std.dev: (222.73, 493.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3694) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1150.782 CPU sec, 51.056 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 642991, 49, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (346, 516, 1018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2362) [M::mem_pestat] mean and std.dev: (687.92, 519.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3034) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.94, 97.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 57, 1443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4293) [M::mem_pestat] mean and std.dev: (656.17, 1141.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5718) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1150.109 CPU sec, 66.331 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643302, 44, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (390, 658, 2421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6483) [M::mem_pestat] mean and std.dev: (1423.69, 1548.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8514) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.95, 97.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 35, 1548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4614) [M::mem_pestat] mean and std.dev: (799.02, 1437.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6549) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1152.405 CPU sec, 51.193 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 642444, 55, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (297, 358, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 885) [M::mem_pestat] mean and std.dev: (313.85, 101.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1081) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.89, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 65, 1330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3944) [M::mem_pestat] mean and std.dev: (607.18, 1021.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5251) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1153.258 CPU sec, 66.492 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 643206, 45, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (347, 451, 851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1859) [M::mem_pestat] mean and std.dev: (576.25, 357.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2363) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.81, 97.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 72, 1977) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5905) [M::mem_pestat] mean and std.dev: (758.29, 1368.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7869) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1163.830 CPU sec, 68.318 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 642717, 46, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.87, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 120, 1070) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3158) [M::mem_pestat] mean and std.dev: (322.71, 643.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4202) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1155.079 CPU sec, 51.233 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 643326, 42, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (404, 1068, 2442) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6518) [M::mem_pestat] mean and std.dev: (1302.31, 1370.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8556) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.00, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 101, 1116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3312) [M::mem_pestat] mean and std.dev: (397.94, 797.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4410) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (324, 845, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2823) [M::mem_pestat] mean and std.dev: (735.92, 489.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3656) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1152.595 CPU sec, 66.570 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643050, 41, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.22, 97.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 41, 116) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (49.94, 51.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (384, 1131, 2189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5799) [M::mem_pestat] mean and std.dev: (1262.09, 1291.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7604) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1152.603 CPU sec, 50.981 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 643135, 38, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.86, 97.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 98, 1684) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4996) [M::mem_pestat] mean and std.dev: (758.34, 1203.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6652) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (833, 966, 2230) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5024) [M::mem_pestat] mean and std.dev: (1080.40, 697.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6421) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1152.315 CPU sec, 67.169 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643055, 39, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (405, 482, 1454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3552) [M::mem_pestat] mean and std.dev: (854.42, 719.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.85, 97.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 96, 1084) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3204) [M::mem_pestat] mean and std.dev: (355.61, 661.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4264) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1151.575 CPU sec, 51.102 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642811, 41, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (380, 485, 1010) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2270) [M::mem_pestat] mean and std.dev: (624.36, 399.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2900) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.16, 97.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 135, 1748) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5198) [M::mem_pestat] mean and std.dev: (751.87, 1233.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6923) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1156.475 CPU sec, 66.679 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643691, 30, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (272, 583, 985) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2411) [M::mem_pestat] mean and std.dev: (654.93, 412.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3124) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.02, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 254, 1726) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5122) [M::mem_pestat] mean and std.dev: (849.41, 1398.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6820) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1151.123 CPU sec, 51.079 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643132, 46, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 531, 1019) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2377) [M::mem_pestat] mean and std.dev: (622.58, 368.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3056) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.08, 97.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 57, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1428) [M::mem_pestat] mean and std.dev: (191.61, 307.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1897) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1146.578 CPU sec, 66.049 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 643236, 45, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.04, 97.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 109, 1217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3603) [M::mem_pestat] mean and std.dev: (398.80, 687.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4796) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1154.000 CPU sec, 51.325 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642877, 46, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (423, 549, 1632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4050) [M::mem_pestat] mean and std.dev: (1171.36, 1078.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5486) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.11, 97.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 44, 1409) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4199) [M::mem_pestat] mean and std.dev: (463.68, 867.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5594) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1158.396 CPU sec, 66.765 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 642795, 45, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.98, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 41, 1394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4146) [M::mem_pestat] mean and std.dev: (236.38, 470.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5522) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1009, 1110, 2051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4135) [M::mem_pestat] mean and std.dev: (1388.80, 893.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5177) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1159.200 CPU sec, 51.522 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 642978, 45, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.30, 97.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 43, 2325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6947) [M::mem_pestat] mean and std.dev: (834.24, 1486.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9258) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (916, 1182, 1674) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3190) [M::mem_pestat] mean and std.dev: (1275.18, 828.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4590) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1157.119 CPU sec, 67.174 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643328, 50, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.02, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 58, 2541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7595) [M::mem_pestat] mean and std.dev: (1223.80, 2013.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10122) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1147.925 CPU sec, 50.712 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643049, 43, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (378, 521, 1492) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3720) [M::mem_pestat] mean and std.dev: (823.25, 721.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4834) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.96, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 87, 1873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5589) [M::mem_pestat] mean and std.dev: (733.33, 1244.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7447) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (386, 797, 1217) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2879) [M::mem_pestat] mean and std.dev: (879.10, 609.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3710) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1155.098 CPU sec, 66.955 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643980, 40, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 468, 2403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6611) [M::mem_pestat] mean and std.dev: (1372.15, 1814.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8715) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.25, 97.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 36, 573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1689) [M::mem_pestat] mean and std.dev: (58.61, 111.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2247) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1152.620 CPU sec, 51.066 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 642781, 44, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (300, 508, 582) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1146) [M::mem_pestat] mean and std.dev: (375.12, 149.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1428) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.06, 97.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 78, 780) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2286) [M::mem_pestat] mean and std.dev: (211.61, 347.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3039) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (626, 1295, 2097) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5039) [M::mem_pestat] mean and std.dev: (1288.44, 854.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6510) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1154.153 CPU sec, 67.852 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 642718, 49, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (231, 860, 1146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2976) [M::mem_pestat] mean and std.dev: (632.33, 518.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3891) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.94, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 35, 211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 603) [M::mem_pestat] mean and std.dev: (65.85, 100.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (911, 1114, 1415) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2423) [M::mem_pestat] mean and std.dev: (1007.44, 313.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2927) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1157.417 CPU sec, 51.329 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643431, 43, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (570, 1116, 2323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5829) [M::mem_pestat] mean and std.dev: (1261.89, 942.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7582) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.99, 97.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 43, 2203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6571) [M::mem_pestat] mean and std.dev: (1064.69, 1684.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8755) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1150.941 CPU sec, 62.286 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643338, 33, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.12, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 40, 1590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4748) [M::mem_pestat] mean and std.dev: (719.88, 1273.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6327) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1162.171 CPU sec, 65.384 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643366, 50, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (407, 682, 1213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2825) [M::mem_pestat] mean and std.dev: (846.73, 641.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.08, 97.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 39, 730) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2150) [M::mem_pestat] mean and std.dev: (241.58, 440.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2860) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1157.209 CPU sec, 51.164 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642799, 49, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (531, 1045, 2558) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6612) [M::mem_pestat] mean and std.dev: (1475.73, 1271.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.95, 97.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 74, 1004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2956) [M::mem_pestat] mean and std.dev: (242.20, 451.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3932) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1153.801 CPU sec, 66.733 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 642759, 53, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (396, 779, 1083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2457) [M::mem_pestat] mean and std.dev: (888.40, 575.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3192) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.01, 97.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 109, 1669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4953) [M::mem_pestat] mean and std.dev: (768.68, 1301.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6595) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1160.956 CPU sec, 51.568 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643568, 44, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (352, 692, 757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1567) [M::mem_pestat] mean and std.dev: (616.00, 250.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1972) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.94, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 87, 3419) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10209) [M::mem_pestat] mean and std.dev: (1850.66, 2858.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13604) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (691, 1139, 1623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3487) [M::mem_pestat] mean and std.dev: (1143.67, 586.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4419) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1159.914 CPU sec, 68.310 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 642938, 42, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.06, 97.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 112, 462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1336) [M::mem_pestat] mean and std.dev: (168.20, 256.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1773) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1158.232 CPU sec, 51.327 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643248, 45, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (380, 487, 970) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2150) [M::mem_pestat] mean and std.dev: (671.10, 527.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2783) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.07, 97.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 88, 1678) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4986) [M::mem_pestat] mean and std.dev: (668.76, 1135.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6640) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1150.429 CPU sec, 66.374 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 642733, 40, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.96, 97.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 32, 84) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 208) [M::mem_pestat] mean and std.dev: (38.71, 29.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 270) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (798, 1517, 4081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10647) [M::mem_pestat] mean and std.dev: (2187.25, 2107.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13930) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1157.092 CPU sec, 51.405 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 642321, 56, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (694, 900, 1755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3877) [M::mem_pestat] mean and std.dev: (1150.22, 907.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4938) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.92, 97.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 96, 845) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2465) [M::mem_pestat] mean and std.dev: (184.00, 335.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3275) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1161.819 CPU sec, 65.704 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 643374, 39, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.95, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 39, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 649) [M::mem_pestat] mean and std.dev: (43.93, 48.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 860) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (852, 927, 1125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (306, 1671) [M::mem_pestat] mean and std.dev: (910.70, 232.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1944) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1164.858 CPU sec, 68.217 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 642941, 35, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.17, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (8, 20, 236) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 692) [M::mem_pestat] mean and std.dev: (38.11, 58.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1160.354 CPU sec, 51.702 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 642802, 50, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (399, 980, 1339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3219) [M::mem_pestat] mean and std.dev: (888.00, 505.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4159) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.82, 97.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 42, 653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1923) [M::mem_pestat] mean and std.dev: (143.83, 287.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2558) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1161.602 CPU sec, 68.724 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642780, 52, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (344, 364, 589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1079) [M::mem_pestat] mean and std.dev: (385.10, 185.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1324) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.23, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 77, 1589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4715) [M::mem_pestat] mean and std.dev: (684.32, 1366.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6278) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1155.096 CPU sec, 51.141 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643283, 48, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (344, 405, 1494) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3794) [M::mem_pestat] mean and std.dev: (731.73, 547.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.02, 97.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 57, 1155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3415) [M::mem_pestat] mean and std.dev: (352.36, 729.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4545) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (852, 1199, 1628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3180) [M::mem_pestat] mean and std.dev: (1053.58, 449.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3956) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1165.010 CPU sec, 68.334 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 642775, 46, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.86, 97.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 89, 2673) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7959) [M::mem_pestat] mean and std.dev: (1363.28, 2096.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10602) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1167.597 CPU sec, 51.798 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643136, 41, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (453, 709, 1295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2979) [M::mem_pestat] mean and std.dev: (898.64, 682.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3821) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.14, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 35, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 961) [M::mem_pestat] mean and std.dev: (66.91, 102.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1277) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (611, 1007, 1891) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4451) [M::mem_pestat] mean and std.dev: (901.55, 472.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5731) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1161.300 CPU sec, 69.290 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 642632, 47, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.88, 96.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 43, 705) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2075) [M::mem_pestat] mean and std.dev: (216.12, 455.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2760) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1159.860 CPU sec, 51.562 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 642877, 44, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.02, 97.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 248, 2936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8760) [M::mem_pestat] mean and std.dev: (1919.82, 2662.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12571) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1167.401 CPU sec, 68.476 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 642909, 37, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.86, 97.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 131, 1228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3624) [M::mem_pestat] mean and std.dev: (444.15, 716.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4822) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (801, 1258, 1602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3204) [M::mem_pestat] mean and std.dev: (1223.80, 492.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4005) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1166.199 CPU sec, 68.118 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643056, 49, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (345, 480, 1178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2844) [M::mem_pestat] mean and std.dev: (534.30, 367.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3677) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.12, 97.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 52, 2579) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7707) [M::mem_pestat] mean and std.dev: (1020.83, 1543.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10271) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1155.949 CPU sec, 51.093 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643076, 49, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (615, 1086, 4903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 13479) [M::mem_pestat] mean and std.dev: (2175.08, 2697.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 17767) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.04, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 36, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2165) [M::mem_pestat] mean and std.dev: (226.09, 457.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2881) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1168.894 CPU sec, 67.389 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 643528, 38, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (370, 889, 2982) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8206) [M::mem_pestat] mean and std.dev: (1687.44, 2002.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10818) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.99, 97.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 49, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1667) [M::mem_pestat] mean and std.dev: (88.27, 167.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2217) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1152.424 CPU sec, 50.933 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 643304, 47, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.91, 97.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 38, 766) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2260) [M::mem_pestat] mean and std.dev: (183.85, 370.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3007) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1158.665 CPU sec, 66.725 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643146, 45, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (353, 456, 1706) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4412) [M::mem_pestat] mean and std.dev: (858.38, 803.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5765) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.86, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 46, 1301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3845) [M::mem_pestat] mean and std.dev: (278.41, 581.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5117) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1148.649 CPU sec, 50.957 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643272, 42, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (519, 608, 1418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3216) [M::mem_pestat] mean and std.dev: (846.10, 667.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4115) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.88, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 55, 1284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3824) [M::mem_pestat] mean and std.dev: (474.92, 868.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5094) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1158.714 CPU sec, 67.556 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643587, 47, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (269, 457, 1447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3803) [M::mem_pestat] mean and std.dev: (640.83, 589.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.91, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 94, 2367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7049) [M::mem_pestat] mean and std.dev: (888.02, 1290.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9390) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1154.988 CPU sec, 51.393 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643120, 49, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.09, 97.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 37, 1391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4149) [M::mem_pestat] mean and std.dev: (617.21, 1015.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5528) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1157.661 CPU sec, 67.105 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642905, 43, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (365, 494, 720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1430) [M::mem_pestat] mean and std.dev: (468.60, 152.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1785) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.89, 96.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 53, 537) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1561) [M::mem_pestat] mean and std.dev: (168.60, 318.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2073) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1153.946 CPU sec, 51.281 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 642831, 46, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (372, 1153, 1691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4329) [M::mem_pestat] mean and std.dev: (1006.75, 707.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5648) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.84, 97.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 43, 2431) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7259) [M::mem_pestat] mean and std.dev: (939.05, 1638.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9673) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1147.817 CPU sec, 65.331 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 642889, 51, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (396, 891, 1688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4272) [M::mem_pestat] mean and std.dev: (672.00, 445.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.95, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 83, 2472) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7364) [M::mem_pestat] mean and std.dev: (1284.69, 1758.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9810) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1153.670 CPU sec, 50.983 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643387, 43, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (651, 828, 1182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2244) [M::mem_pestat] mean and std.dev: (832.40, 387.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2775) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.09, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (39, 87, 918) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2676) [M::mem_pestat] mean and std.dev: (424.97, 634.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3555) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (872, 2102, 4466) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11654) [M::mem_pestat] mean and std.dev: (2419.36, 1978.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15248) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1147.153 CPU sec, 65.605 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 643102, 52, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 510, 2879) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8095) [M::mem_pestat] mean and std.dev: (1551.15, 2044.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10703) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.99, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 90, 1156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3418) [M::mem_pestat] mean and std.dev: (459.15, 750.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4549) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1153.940 CPU sec, 51.272 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 643454, 52, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.95, 97.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 163, 1955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5825) [M::mem_pestat] mean and std.dev: (1023.72, 1464.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7760) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1146.959 CPU sec, 66.167 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643739, 42, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.97, 97.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 49, 2190) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6530) [M::mem_pestat] mean and std.dev: (896.50, 1398.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8700) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1156.900 CPU sec, 51.276 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643460, 38, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.85, 97.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 123, 3914) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11676) [M::mem_pestat] mean and std.dev: (1633.68, 2540.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15557) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (731, 2030, 2564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6230) [M::mem_pestat] mean and std.dev: (1604.00, 948.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8063) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1148.564 CPU sec, 65.188 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643746, 49, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.14, 97.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 144, 2510) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7492) [M::mem_pestat] mean and std.dev: (1275.10, 1786.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9983) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1146.188 CPU sec, 50.953 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 642881, 55, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.88, 97.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 47, 451) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1311) [M::mem_pestat] mean and std.dev: (107.09, 160.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1741) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1149.818 CPU sec, 59.484 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 643347, 47, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (331, 493, 1069) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2545) [M::mem_pestat] mean and std.dev: (588.79, 353.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3283) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.97, 97.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 140, 2209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6573) [M::mem_pestat] mean and std.dev: (1004.24, 1507.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8755) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1145.405 CPU sec, 62.289 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 642701, 43, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (297, 581, 1016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2454) [M::mem_pestat] mean and std.dev: (570.44, 343.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3173) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.94, 97.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 59, 386) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (100.56, 189.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1487) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (903, 1213, 2265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4989) [M::mem_pestat] mean and std.dev: (1110.00, 584.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6351) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1156.514 CPU sec, 51.201 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643030, 35, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (362, 1448, 2718) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7430) [M::mem_pestat] mean and std.dev: (1607.07, 1492.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.02, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 59, 1671) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4961) [M::mem_pestat] mean and std.dev: (820.00, 1380.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6606) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (851, 923, 1717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3449) [M::mem_pestat] mean and std.dev: (935.09, 504.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4315) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1147.521 CPU sec, 64.370 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 642934, 51, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 458, 813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1763) [M::mem_pestat] mean and std.dev: (511.43, 279.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2238) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.25, 97.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 162, 2986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8916) [M::mem_pestat] mean and std.dev: (1901.18, 2571.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12189) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1148.574 CPU sec, 50.847 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 642888, 32, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.85, 97.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 39, 2765) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8263) [M::mem_pestat] mean and std.dev: (965.43, 1712.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11012) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1152.900 CPU sec, 65.349 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 643074, 50, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 391, 641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1395) [M::mem_pestat] mean and std.dev: (391.31, 214.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1772) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.88, 97.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 48, 688) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2020) [M::mem_pestat] mean and std.dev: (234.47, 473.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2686) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1154.517 CPU sec, 51.253 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 643101, 54, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 441, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 847) [M::mem_pestat] mean and std.dev: (339.07, 164.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1039) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.79, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 94, 828) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2440) [M::mem_pestat] mean and std.dev: (118.44, 190.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3246) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1146.290 CPU sec, 65.084 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642645, 49, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (354, 543, 1156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2760) [M::mem_pestat] mean and std.dev: (759.60, 612.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3562) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.86, 97.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 80, 1834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5462) [M::mem_pestat] mean and std.dev: (836.23, 1441.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7276) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1149.485 CPU sec, 50.904 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643113, 47, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (298, 597, 1339) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3421) [M::mem_pestat] mean and std.dev: (842.62, 678.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4462) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.00, 97.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 107, 1739) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5177) [M::mem_pestat] mean and std.dev: (876.71, 1305.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6896) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1159.355 CPU sec, 63.590 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 643415, 53, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (486, 632, 922) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1794) [M::mem_pestat] mean and std.dev: (676.21, 426.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2382) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.25, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 74, 2247) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6675) [M::mem_pestat] mean and std.dev: (1047.96, 1731.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8889) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1155.901 CPU sec, 65.637 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643659, 45, 0) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (496, 973, 1714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4150) [M::mem_pestat] mean and std.dev: (1077.25, 913.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5368) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.89, 97.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 132, 2711) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8071) [M::mem_pestat] mean and std.dev: (1266.58, 1767.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10751) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1157.733 CPU sec, 51.408 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 642958, 49, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (347, 835, 1262) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3092) [M::mem_pestat] mean and std.dev: (730.89, 418.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4007) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.96, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (50, 126, 3073) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9119) [M::mem_pestat] mean and std.dev: (1711.35, 2544.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12142) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1164.876 CPU sec, 68.525 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 642781, 36, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (348, 519, 1710) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4434) [M::mem_pestat] mean and std.dev: (922.57, 962.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5796) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.99, 97.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 76, 2283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6789) [M::mem_pestat] mean and std.dev: (998.29, 1791.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9042) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (824, 1183, 1995) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4337) [M::mem_pestat] mean and std.dev: (1434.77, 949.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5508) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1159.576 CPU sec, 51.418 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643460, 47, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (643, 829, 1564) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3406) [M::mem_pestat] mean and std.dev: (980.30, 513.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.04, 97.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 52, 1457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4333) [M::mem_pestat] mean and std.dev: (469.62, 866.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5771) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (727, 1684, 2622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6412) [M::mem_pestat] mean and std.dev: (1481.89, 1011.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8307) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1158.299 CPU sec, 67.198 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 642859, 40, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (403, 959, 1462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3580) [M::mem_pestat] mean and std.dev: (853.83, 502.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4639) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.92, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 522, 3394) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10128) [M::mem_pestat] mean and std.dev: (1721.03, 2285.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13495) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1164.346 CPU sec, 51.705 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643489, 49, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (367, 400, 800) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1666) [M::mem_pestat] mean and std.dev: (477.56, 242.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2099) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.17, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 70, 2655) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7907) [M::mem_pestat] mean and std.dev: (991.32, 1541.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10533) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1162.833 CPU sec, 69.140 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642746, 30, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (329, 594, 1189) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2909) [M::mem_pestat] mean and std.dev: (869.55, 802.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4081) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.90, 97.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 167, 1175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3487) [M::mem_pestat] mean and std.dev: (521.85, 897.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4643) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (954, 1281, 2694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6174) [M::mem_pestat] mean and std.dev: (1402.10, 910.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7914) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1159.632 CPU sec, 51.568 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643218, 52, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (358, 623, 1166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2782) [M::mem_pestat] mean and std.dev: (769.18, 534.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3590) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.93, 97.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 122, 2574) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7684) [M::mem_pestat] mean and std.dev: (976.92, 1529.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10239) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (567, 1619, 2921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7629) [M::mem_pestat] mean and std.dev: (1805.20, 1463.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9983) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1151.659 CPU sec, 66.639 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 642981, 53, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (326, 424, 801) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1751) [M::mem_pestat] mean and std.dev: (517.85, 344.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2226) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.02, 97.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 166, 2603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7767) [M::mem_pestat] mean and std.dev: (1200.94, 1742.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10349) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (925, 1302, 2252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4906) [M::mem_pestat] mean and std.dev: (1368.54, 773.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6233) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1161.242 CPU sec, 67.843 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643551, 39, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (774, 1221, 1853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4011) [M::mem_pestat] mean and std.dev: (1195.09, 858.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5090) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.11, 97.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 57, 1395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4141) [M::mem_pestat] mean and std.dev: (525.46, 1021.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5514) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1158.215 CPU sec, 51.449 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 643344, 48, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (393, 651, 1183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2763) [M::mem_pestat] mean and std.dev: (789.38, 574.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3553) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.07, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 48, 1694) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5050) [M::mem_pestat] mean and std.dev: (709.33, 1345.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6728) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1158.832 CPU sec, 67.027 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 642955, 44, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (378, 970, 1260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3024) [M::mem_pestat] mean and std.dev: (1009.86, 806.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4236) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.94, 97.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 48, 1216) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3606) [M::mem_pestat] mean and std.dev: (419.61, 870.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4801) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (785, 1164, 1349) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2477) [M::mem_pestat] mean and std.dev: (1083.80, 543.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3260) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1157.284 CPU sec, 51.378 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 643290, 49, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (368, 632, 1346) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3302) [M::mem_pestat] mean and std.dev: (886.28, 660.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4280) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.04, 97.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 31, 234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 666) [M::mem_pestat] mean and std.dev: (71.37, 93.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 882) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1157.164 CPU sec, 66.457 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 642973, 44, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (384, 866, 1422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3498) [M::mem_pestat] mean and std.dev: (823.80, 571.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4536) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.13, 97.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 41, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1435) [M::mem_pestat] mean and std.dev: (105.11, 201.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1906) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1156.149 CPU sec, 51.200 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643322, 49, 14) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (409, 456, 1322) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3148) [M::mem_pestat] mean and std.dev: (635.60, 405.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.99, 97.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 148, 2130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6350) [M::mem_pestat] mean and std.dev: (925.66, 1513.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8460) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (646, 870, 1632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3604) [M::mem_pestat] mean and std.dev: (965.62, 521.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4590) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1153.346 CPU sec, 66.946 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 642641, 41, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.84, 97.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 495, 2443) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7289) [M::mem_pestat] mean and std.dev: (1145.12, 1498.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9712) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1159.131 CPU sec, 51.591 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 643580, 53, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (373, 812, 1383) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3403) [M::mem_pestat] mean and std.dev: (919.67, 669.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4413) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.10, 97.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 50, 1422) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4224) [M::mem_pestat] mean and std.dev: (587.12, 957.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5625) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1164.228 CPU sec, 69.133 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 642935, 54, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 365, 865) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1997) [M::mem_pestat] mean and std.dev: (536.73, 368.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2563) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.00, 97.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 35, 119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (30.15, 27.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 449) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1154.084 CPU sec, 51.219 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 643079, 37, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.05, 97.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 237, 1983) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5893) [M::mem_pestat] mean and std.dev: (827.82, 1184.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7848) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1171.128 CPU sec, 68.678 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 643326, 38, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.94, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (47, 434, 2037) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6017) [M::mem_pestat] mean and std.dev: (1099.11, 1371.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8007) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1150.276 CPU sec, 50.950 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 643190, 60, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.94, 97.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 51, 486) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1430) [M::mem_pestat] mean and std.dev: (109.73, 203.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1902) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1157.167 CPU sec, 66.493 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 642587, 37, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (294, 591, 2121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5775) [M::mem_pestat] mean and std.dev: (1397.67, 1479.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7602) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.03, 97.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 49, 630) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1846) [M::mem_pestat] mean and std.dev: (126.40, 277.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2454) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1148.509 CPU sec, 50.772 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643111, 54, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.15, 97.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 148, 1687) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4997) [M::mem_pestat] mean and std.dev: (777.33, 1269.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6652) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1155.079 CPU sec, 67.868 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643105, 50, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 753, 1533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3931) [M::mem_pestat] mean and std.dev: (912.17, 812.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5130) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.02, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 44, 215) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 617) [M::mem_pestat] mean and std.dev: (46.03, 54.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 818) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1158.585 CPU sec, 51.421 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 642748, 44, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.95, 97.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (33, 164, 2410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7164) [M::mem_pestat] mean and std.dev: (1045.92, 1665.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9541) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1159.061 CPU sec, 66.972 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 642802, 41, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.97, 97.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 92, 1928) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5746) [M::mem_pestat] mean and std.dev: (1012.33, 1483.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7655) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1157.329 CPU sec, 51.374 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (17, 643023, 40, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (287, 408, 835) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1931) [M::mem_pestat] mean and std.dev: (477.60, 379.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2479) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.87, 97.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 90, 1427) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4231) [M::mem_pestat] mean and std.dev: (517.94, 831.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5633) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1153.156 CPU sec, 57.815 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 642757, 48, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.18, 97.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 46, 703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2081) [M::mem_pestat] mean and std.dev: (219.45, 471.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2770) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (272, 1163, 1314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3398) [M::mem_pestat] mean and std.dev: (846.22, 632.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4440) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1152.961 CPU sec, 65.049 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 643349, 41, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.11, 97.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 98, 2407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7165) [M::mem_pestat] mean and std.dev: (957.46, 1474.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9544) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1156.913 CPU sec, 51.181 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 642949, 41, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 432, 825) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2027) [M::mem_pestat] mean and std.dev: (520.50, 393.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2628) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.07, 97.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 64, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1023) [M::mem_pestat] mean and std.dev: (62.16, 77.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1358) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1145.342 CPU sec, 64.817 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643769, 49, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (326, 461, 734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1550) [M::mem_pestat] mean and std.dev: (494.82, 204.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1958) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.11, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 77, 1541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4571) [M::mem_pestat] mean and std.dev: (674.78, 1192.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6086) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (756, 1468, 2157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4959) [M::mem_pestat] mean and std.dev: (1521.00, 1190.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6360) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1150.702 CPU sec, 50.953 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 642574, 40, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (485, 682, 1355) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3095) [M::mem_pestat] mean and std.dev: (740.08, 454.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.09, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 55, 480) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1406) [M::mem_pestat] mean and std.dev: (118.09, 253.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1869) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1150.293 CPU sec, 66.653 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 642738, 55, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (236, 390, 3068) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8732) [M::mem_pestat] mean and std.dev: (1224.20, 1347.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11564) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.94, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 117, 3757) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11207) [M::mem_pestat] mean and std.dev: (1887.65, 2669.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14932) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (412, 1139, 1495) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3661) [M::mem_pestat] mean and std.dev: (1025.40, 493.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4744) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1145.977 CPU sec, 50.673 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643789, 50, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (391, 636, 1265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3013) [M::mem_pestat] mean and std.dev: (710.36, 492.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3887) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.08, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 58, 1624) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4824) [M::mem_pestat] mean and std.dev: (666.71, 1192.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6424) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1152.437 CPU sec, 66.014 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643184, 51, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (361, 536, 1378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3412) [M::mem_pestat] mean and std.dev: (751.40, 694.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4429) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.08, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 78, 1086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3216) [M::mem_pestat] mean and std.dev: (346.28, 639.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4281) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (915, 1213, 2016) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4218) [M::mem_pestat] mean and std.dev: (1213.60, 600.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5319) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1145.968 CPU sec, 50.630 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643199, 48, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (348, 983, 1391) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3477) [M::mem_pestat] mean and std.dev: (890.77, 652.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4520) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.92, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 73, 1827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5445) [M::mem_pestat] mean and std.dev: (704.80, 1350.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7254) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (902, 1063, 1539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2813) [M::mem_pestat] mean and std.dev: (1193.73, 515.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3450) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1151.561 CPU sec, 62.766 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643700, 35, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (298, 411, 783) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1753) [M::mem_pestat] mean and std.dev: (533.36, 346.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2238) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.16, 97.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 124, 851) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2507) [M::mem_pestat] mean and std.dev: (370.33, 620.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3335) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (861, 1446, 2008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4302) [M::mem_pestat] mean and std.dev: (1157.36, 744.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5449) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1148.719 CPU sec, 66.336 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643001, 34, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (344, 478, 1064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2504) [M::mem_pestat] mean and std.dev: (633.92, 417.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3224) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.07, 97.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 42, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 398) [M::mem_pestat] mean and std.dev: (50.19, 59.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 526) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1145.843 CPU sec, 50.798 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 642104, 53, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 1019, 1135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2877) [M::mem_pestat] mean and std.dev: (835.22, 662.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3748) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.03, 97.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 83, 1175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3467) [M::mem_pestat] mean and std.dev: (381.00, 693.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4613) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1148.966 CPU sec, 66.134 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (21, 642892, 39, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (376, 844, 1449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3595) [M::mem_pestat] mean and std.dev: (827.74, 520.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4668) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.01, 97.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 67, 1320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3892) [M::mem_pestat] mean and std.dev: (562.08, 900.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5178) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1154.054 CPU sec, 51.246 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643245, 52, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.00, 97.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 60, 1784) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5292) [M::mem_pestat] mean and std.dev: (823.84, 1361.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7046) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1143.340 CPU sec, 64.761 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 642759, 43, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (437, 583, 1106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2444) [M::mem_pestat] mean and std.dev: (656.64, 412.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3113) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.92, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 61, 1583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4693) [M::mem_pestat] mean and std.dev: (943.23, 1486.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6890) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1149.345 CPU sec, 50.891 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 643316, 51, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.23, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 54, 1018) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2994) [M::mem_pestat] mean and std.dev: (269.86, 552.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3982) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1062, 1297, 1924) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3648) [M::mem_pestat] mean and std.dev: (1413.91, 867.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4883) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1147.170 CPU sec, 65.121 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 642942, 46, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (407, 515, 1761) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4469) [M::mem_pestat] mean and std.dev: (972.27, 740.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5823) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.12, 96.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 52, 1722) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5118) [M::mem_pestat] mean and std.dev: (841.91, 1511.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6889) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1143.802 CPU sec, 50.613 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643146, 34, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (332, 762, 1155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2801) [M::mem_pestat] mean and std.dev: (651.27, 434.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.07, 97.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 554, 3373) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 10059) [M::mem_pestat] mean and std.dev: (1991.03, 2612.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 13402) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1146.896 CPU sec, 65.563 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 642783, 38, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 727, 1557) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4003) [M::mem_pestat] mean and std.dev: (799.85, 500.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5226) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.98, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 74, 261) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 735) [M::mem_pestat] mean and std.dev: (101.47, 140.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 972) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1159.886 CPU sec, 68.502 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 642405, 43, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (423, 560, 3149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8601) [M::mem_pestat] mean and std.dev: (1568.00, 1989.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11327) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.17, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 98, 2635) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7859) [M::mem_pestat] mean and std.dev: (1267.83, 2044.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10471) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (705, 894, 1651) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3543) [M::mem_pestat] mean and std.dev: (925.78, 493.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4489) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1158.503 CPU sec, 51.361 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 642923, 50, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (380, 770, 1441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3563) [M::mem_pestat] mean and std.dev: (1032.00, 857.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4624) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.16, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 182, 2568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7656) [M::mem_pestat] mean and std.dev: (1128.10, 1595.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10200) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1154.183 CPU sec, 66.977 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 642857, 47, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.16, 97.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 54, 1504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4454) [M::mem_pestat] mean and std.dev: (633.93, 1044.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5929) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1148.012 CPU sec, 50.893 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643114, 43, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (436, 904, 2099) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5425) [M::mem_pestat] mean and std.dev: (953.92, 783.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7088) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.93, 97.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 60, 2048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6118) [M::mem_pestat] mean and std.dev: (1080.58, 1573.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8153) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1148.825 CPU sec, 65.491 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (19, 643374, 46, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (375, 504, 1208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2874) [M::mem_pestat] mean and std.dev: (790.56, 682.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.21, 97.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 134, 2133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6345) [M::mem_pestat] mean and std.dev: (768.26, 1322.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8451) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1148.883 CPU sec, 50.766 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 642396, 47, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.87, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 126, 1872) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5552) [M::mem_pestat] mean and std.dev: (928.42, 1528.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7392) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1154.507 CPU sec, 67.513 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643310, 46, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (269, 470, 625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1337) [M::mem_pestat] mean and std.dev: (422.75, 204.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1693) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.07, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 144, 1707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5071) [M::mem_pestat] mean and std.dev: (846.57, 1301.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6753) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (736, 1386, 1900) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4228) [M::mem_pestat] mean and std.dev: (1289.11, 675.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5392) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1150.136 CPU sec, 50.798 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643118, 42, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (324, 367, 601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1155) [M::mem_pestat] mean and std.dev: (368.11, 143.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1432) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.91, 97.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 55, 1144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3392) [M::mem_pestat] mean and std.dev: (377.76, 751.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4516) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1150.442 CPU sec, 67.208 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 642665, 39, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (513, 907, 1806) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4392) [M::mem_pestat] mean and std.dev: (1002.25, 622.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5685) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.04, 97.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 52, 1814) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5414) [M::mem_pestat] mean and std.dev: (843.76, 1375.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7214) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1162.987 CPU sec, 66.538 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 643143, 53, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (269, 1184, 1639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4379) [M::mem_pestat] mean and std.dev: (911.93, 720.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5749) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.03, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 84, 1462) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4332) [M::mem_pestat] mean and std.dev: (401.76, 746.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5767) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1152.800 CPU sec, 51.083 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 642811, 38, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.88, 97.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 46, 2239) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6691) [M::mem_pestat] mean and std.dev: (1024.75, 1680.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8917) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1149.788 CPU sec, 65.114 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 643147, 39, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (293, 982, 1996) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5402) [M::mem_pestat] mean and std.dev: (1284.30, 1206.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7105) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.03, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 89, 2085) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6215) [M::mem_pestat] mean and std.dev: (755.29, 1281.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8280) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1143.500 CPU sec, 50.669 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 642999, 54, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.03, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 38, 1098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3272) [M::mem_pestat] mean and std.dev: (262.59, 621.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4359) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1147.631 CPU sec, 66.357 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 642853, 50, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (393, 543, 959) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2091) [M::mem_pestat] mean and std.dev: (659.00, 425.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2657) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.12, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 47, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1076) [M::mem_pestat] mean and std.dev: (77.92, 123.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1427) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1144.726 CPU sec, 50.648 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 642704, 49, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (909, 1122, 1575) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2907) [M::mem_pestat] mean and std.dev: (1112.07, 579.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3573) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.13, 97.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 41, 839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2483) [M::mem_pestat] mean and std.dev: (301.33, 570.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3305) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1147.810 CPU sec, 66.789 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643446, 43, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (445, 844, 1377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3241) [M::mem_pestat] mean and std.dev: (1040.18, 731.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4173) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.99, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 47, 1308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3888) [M::mem_pestat] mean and std.dev: (617.05, 1079.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5178) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1147.543 CPU sec, 50.662 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 643763, 52, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.06, 97.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 90, 2493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7423) [M::mem_pestat] mean and std.dev: (1215.42, 1722.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9888) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1148.746 CPU sec, 66.049 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 643691, 41, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (441, 1309, 2479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6555) [M::mem_pestat] mean and std.dev: (1338.67, 1016.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8593) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.92, 97.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 58, 1058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3138) [M::mem_pestat] mean and std.dev: (197.55, 481.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4178) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (867, 1169, 1529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2853) [M::mem_pestat] mean and std.dev: (1103.42, 539.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3515) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1147.367 CPU sec, 50.796 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643365, 49, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.07, 97.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 56, 1836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5466) [M::mem_pestat] mean and std.dev: (692.70, 1142.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7281) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1166.950 CPU sec, 69.761 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 643090, 43, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (358, 1360, 2253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6043) [M::mem_pestat] mean and std.dev: (1340.92, 1237.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7938) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.97, 97.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 56, 1869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5569) [M::mem_pestat] mean and std.dev: (715.77, 1305.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7419) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1165.231 CPU sec, 51.696 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643009, 51, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.92, 97.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 60, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1285) [M::mem_pestat] mean and std.dev: (126.93, 199.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1704) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1157.737 CPU sec, 68.205 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 642941, 45, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (244, 490, 842) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2038) [M::mem_pestat] mean and std.dev: (456.92, 285.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.96, 97.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 43, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (63.38, 84.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1155.899 CPU sec, 51.190 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 643078, 49, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.91, 97.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 43, 1881) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5607) [M::mem_pestat] mean and std.dev: (840.44, 1399.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7470) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1156.323 CPU sec, 66.757 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 643276, 46, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (252, 295, 736) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1704) [M::mem_pestat] mean and std.dev: (453.46, 302.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2188) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.19, 97.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 101, 1753) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5201) [M::mem_pestat] mean and std.dev: (814.60, 1372.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6925) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1151.923 CPU sec, 51.014 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 643514, 42, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (491, 663, 1109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2345) [M::mem_pestat] mean and std.dev: (700.53, 338.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2963) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.01, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 59, 2328) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6942) [M::mem_pestat] mean and std.dev: (893.25, 1419.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9249) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1148.721 CPU sec, 67.252 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 642820, 49, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (367, 594, 871) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1879) [M::mem_pestat] mean and std.dev: (568.31, 318.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2383) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.14, 96.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 71, 1529) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4551) [M::mem_pestat] mean and std.dev: (524.51, 921.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6062) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1150.204 CPU sec, 51.125 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643342, 38, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (270, 440, 1191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3033) [M::mem_pestat] mean and std.dev: (700.09, 558.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3954) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.96, 97.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 98, 667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1969) [M::mem_pestat] mean and std.dev: (213.94, 370.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2620) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1141.900 CPU sec, 59.226 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (20, 643465, 40, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (388, 983, 2621) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7087) [M::mem_pestat] mean and std.dev: (1241.00, 1274.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9320) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.97, 97.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 81, 1122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3330) [M::mem_pestat] mean and std.dev: (368.91, 708.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4434) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1148.973 CPU sec, 63.429 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 642715, 39, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (320, 886, 2301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6263) [M::mem_pestat] mean and std.dev: (1457.20, 1734.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8393) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (442.03, 97.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (36, 93, 1508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4452) [M::mem_pestat] mean and std.dev: (851.31, 1361.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6296) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (725, 1048, 1580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3290) [M::mem_pestat] mean and std.dev: (1113.33, 716.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4145) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1149.975 CPU sec, 50.938 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 642799, 54, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (286, 365, 561) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1111) [M::mem_pestat] mean and std.dev: (401.42, 222.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1386) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.95, 97.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 42, 902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2676) [M::mem_pestat] mean and std.dev: (256.04, 513.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3563) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1153.270 CPU sec, 66.122 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 643641, 52, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.02, 97.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 40, 1251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3729) [M::mem_pestat] mean and std.dev: (520.04, 946.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4968) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1147.982 CPU sec, 50.911 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 642910, 40, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 687, 1095) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2609) [M::mem_pestat] mean and std.dev: (682.94, 582.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3366) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.12, 97.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 42, 1428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4246) [M::mem_pestat] mean and std.dev: (404.06, 894.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5655) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (452, 760, 1441) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3419) [M::mem_pestat] mean and std.dev: (795.50, 598.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4408) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1146.531 CPU sec, 65.369 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 642812, 41, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (285, 619, 1436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3738) [M::mem_pestat] mean and std.dev: (701.54, 560.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4889) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.06, 97.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 54, 1668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4964) [M::mem_pestat] mean and std.dev: (562.92, 1175.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6612) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1148.282 CPU sec, 50.858 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 643191, 47, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (593, 818, 2752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7070) [M::mem_pestat] mean and std.dev: (1630.27, 1735.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9229) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.05, 97.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 45, 1578) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4680) [M::mem_pestat] mean and std.dev: (811.62, 1218.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6231) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1589404 sequences (240000004 bp)... [M::mem_process_seqs] Processed 1589404 reads in 1143.580 CPU sec, 64.448 real sec [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 643215, 54, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 524, 1622) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4232) [M::mem_pestat] mean and std.dev: (704.25, 779.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5537) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 436, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (442.17, 96.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 159, 2400) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7144) [M::mem_pestat] mean and std.dev: (1075.68, 1547.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9516) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1589404 reads in 1139.872 CPU sec, 50.459 real sec [M::process] read 1589404 sequences (240000004 bp)... [M::process] 0 single-end sequences; 1589404 paired-end sequences [M::process] read 1004386 sequences (151662286 bp)... [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 642644, 41, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (311, 682, 1354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3440) [M::mem_pestat] mean and std.dev: (938.20, 757.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4483) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (376, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 757) [M::mem_pestat] mean and std.dev: (441.94, 97.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 884) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 83, 1567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4663) [M::mem_pestat] mean and std.dev: (693.51, 1297.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6211) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (818, 974, 1773) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3683) [M::mem_pestat] mean and std.dev: (1344.64, 815.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4638) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1589404 reads in 1148.270 CPU sec, 60.609 real sec [M::process] 0 single-end sequences; 1004386 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 406477, 23, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (377, 435, 503) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 755) [M::mem_pestat] mean and std.dev: (441.76, 97.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 881) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 109, 2570) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7660) [M::mem_pestat] mean and std.dev: (1253.27, 1896.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10205) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1004386 reads in 717.195 CPU sec, 31.419 real sec [main] Version: 0.7.17-r1188 [main] CMD: /home/csyk2proj/tools/bwa.kit/bwa mem -p -t24 -R@RG\tID:KPGP-00343\tPL:ILLUMINA\tSM:KPGP-00343 -C /home/csyk2proj/data/bwa.kit/hs38DH.fa - [main] Real time: 51622.244 sec; CPU: 643203.278 sec