[M::bwa_idx_load_from_disk] read 3171 ALT contigs [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 659862, 43, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.11, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 29, 1240) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3686) [M::mem_pestat] mean and std.dev: (595.73, 1047.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4909) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (729, 1189, 3682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9588) [M::mem_pestat] mean and std.dev: (2012.40, 2048.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 12541) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1170.345 CPU sec, 67.209 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660133, 69, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.10, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 37, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (51.12, 68.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1151.403 CPU sec, 50.972 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660073, 52, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.16, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 39, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 802) [M::mem_pestat] mean and std.dev: (53.76, 73.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1063) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1154.551 CPU sec, 64.305 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659679, 48, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.15, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 51, 1058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3128) [M::mem_pestat] mean and std.dev: (279.40, 498.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4163) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1153.422 CPU sec, 51.045 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659349, 45, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.10, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 63, 1197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3539) [M::mem_pestat] mean and std.dev: (438.60, 842.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4710) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1151.203 CPU sec, 61.550 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659807, 57, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (332, 504, 989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2303) [M::mem_pestat] mean and std.dev: (588.44, 322.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2960) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.11, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 34, 141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (37.11, 42.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 519) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1146.210 CPU sec, 50.609 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (18, 660146, 51, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 623, 1222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3160) [M::mem_pestat] mean and std.dev: (668.56, 550.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4129) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.21, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 42, 340) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 996) [M::mem_pestat] mean and std.dev: (65.24, 111.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1324) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1144.752 CPU sec, 62.226 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659707, 48, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (328, 641, 941) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2167) [M::mem_pestat] mean and std.dev: (691.00, 509.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2780) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 391, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.12, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 76, 1580) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4690) [M::mem_pestat] mean and std.dev: (655.41, 1102.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6245) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1143.129 CPU sec, 50.516 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 659600, 64, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (361, 621, 1750) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4528) [M::mem_pestat] mean and std.dev: (866.31, 613.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5917) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.25, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 45, 836) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2472) [M::mem_pestat] mean and std.dev: (298.34, 525.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3290) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1140.526 CPU sec, 62.015 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660145, 45, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.23, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 41, 174) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (48.81, 53.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 639) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1146.502 CPU sec, 63.916 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 660139, 54, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.16, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 33, 489) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1437) [M::mem_pestat] mean and std.dev: (149.20, 298.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1911) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1141.292 CPU sec, 50.401 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660023, 58, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 813, 1407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3553) [M::mem_pestat] mean and std.dev: (708.50, 455.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.16, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 40, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (54.91, 84.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 741) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.107 CPU sec, 60.048 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 659930, 65, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (476, 1061, 2135) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5453) [M::mem_pestat] mean and std.dev: (1296.30, 1410.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7112) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.27, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 43, 265) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 759) [M::mem_pestat] mean and std.dev: (63.23, 75.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1006) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1146.499 CPU sec, 50.772 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659796, 52, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.29, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 61, 1161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3447) [M::mem_pestat] mean and std.dev: (408.70, 732.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4590) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.090 CPU sec, 60.184 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659660, 59, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.19, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 39, 95) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 249) [M::mem_pestat] mean and std.dev: (36.81, 37.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 326) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1138.526 CPU sec, 50.142 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659669, 48, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.35, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 75, 654) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1906) [M::mem_pestat] mean and std.dev: (138.72, 214.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2532) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1146.122 CPU sec, 60.842 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659481, 54, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.19, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 39, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 984) [M::mem_pestat] mean and std.dev: (48.85, 71.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1307) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1136.523 CPU sec, 50.150 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659664, 46, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (456, 1464, 2341) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6111) [M::mem_pestat] mean and std.dev: (1561.60, 1390.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7996) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.40, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 39, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 339) [M::mem_pestat] mean and std.dev: (40.49, 46.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 446) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.559 CPU sec, 60.025 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 660023, 54, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.23, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 47, 295) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 851) [M::mem_pestat] mean and std.dev: (100.93, 166.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1129) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1138.902 CPU sec, 50.494 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659867, 45, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.34, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 43, 1064) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3150) [M::mem_pestat] mean and std.dev: (302.45, 550.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4193) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.154 CPU sec, 61.504 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 659576, 55, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (323, 407, 1316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3302) [M::mem_pestat] mean and std.dev: (569.36, 443.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4295) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.33, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 46, 164) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 454) [M::mem_pestat] mean and std.dev: (51.00, 69.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 599) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1145.523 CPU sec, 50.708 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 659490, 59, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.27, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 43, 1060) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3138) [M::mem_pestat] mean and std.dev: (356.85, 738.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4177) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1129.905 CPU sec, 60.750 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 659710, 42, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.53, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 40, 759) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2237) [M::mem_pestat] mean and std.dev: (202.91, 470.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2976) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (270, 1227, 1325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3435) [M::mem_pestat] mean and std.dev: (747.12, 491.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4490) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1135.981 CPU sec, 50.198 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659678, 63, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 38, 976) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2894) [M::mem_pestat] mean and std.dev: (288.67, 609.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3853) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1132.466 CPU sec, 60.104 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660007, 62, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.27, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 43, 1906) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5676) [M::mem_pestat] mean and std.dev: (877.80, 1343.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7561) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1138.997 CPU sec, 50.296 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659999, 64, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.19, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 35, 1149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3417) [M::mem_pestat] mean and std.dev: (226.96, 541.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4551) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.358 CPU sec, 60.068 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660398, 52, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 670) [M::mem_pestat] mean and std.dev: (396.33, 82.87) [M::mem_pestat] low and high boundaries for proper pairs: (10, 780) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 35, 316) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 926) [M::mem_pestat] mean and std.dev: (75.00, 138.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1231) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.022 CPU sec, 49.697 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 660819, 41, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.11, 82.47) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 45, 535) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1569) [M::mem_pestat] mean and std.dev: (164.97, 318.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2086) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (444, 1396, 2357) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6183) [M::mem_pestat] mean and std.dev: (1564.08, 1567.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8096) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1123.830 CPU sec, 60.025 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660420, 54, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 670) [M::mem_pestat] mean and std.dev: (396.22, 82.92) [M::mem_pestat] low and high boundaries for proper pairs: (10, 780) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 38, 304) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 872) [M::mem_pestat] mean and std.dev: (51.22, 65.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1156) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.215 CPU sec, 49.940 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660399, 55, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 47, 613) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1809) [M::mem_pestat] mean and std.dev: (195.89, 413.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2407) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (362, 1148, 1296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3164) [M::mem_pestat] mean and std.dev: (888.33, 450.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4098) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.498 CPU sec, 60.132 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660423, 50, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.27, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 47, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 346) [M::mem_pestat] mean and std.dev: (37.26, 32.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 456) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.989 CPU sec, 49.964 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659828, 58, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.18, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 49, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (52.82, 52.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 788) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1131.094 CPU sec, 60.528 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 660351, 33, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 393, 824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2078) [M::mem_pestat] mean and std.dev: (353.75, 220.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2705) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (395.99, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 53, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 822) [M::mem_pestat] mean and std.dev: (86.32, 162.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1089) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.798 CPU sec, 49.987 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660284, 58, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.13, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 37, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1197) [M::mem_pestat] mean and std.dev: (56.39, 91.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1592) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.559 CPU sec, 63.799 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659788, 46, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.15, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 28, 64) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 164) [M::mem_pestat] mean and std.dev: (26.08, 19.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 214) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.621 CPU sec, 50.026 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660054, 42, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.28, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 64, 1205) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3553) [M::mem_pestat] mean and std.dev: (516.28, 858.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4727) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.728 CPU sec, 59.762 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 659921, 46, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.12, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 54, 2352) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7018) [M::mem_pestat] mean and std.dev: (937.95, 1545.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9351) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.870 CPU sec, 50.063 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 659667, 45, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (458, 1238, 2024) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5156) [M::mem_pestat] mean and std.dev: (1415.71, 1231.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.26, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 28, 127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (35.39, 32.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 457) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.857 CPU sec, 62.453 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659939, 59, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.11, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 29, 176) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 498) [M::mem_pestat] mean and std.dev: (41.63, 67.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 659) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1134.283 CPU sec, 50.172 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 660053, 47, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (213, 241, 1686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4632) [M::mem_pestat] mean and std.dev: (470.91, 513.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6105) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.13, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 33, 418) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1228) [M::mem_pestat] mean and std.dev: (68.97, 139.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1633) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.214 CPU sec, 63.850 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 659925, 54, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.18, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 24, 65) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 171) [M::mem_pestat] mean and std.dev: (26.73, 23.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 224) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1135.377 CPU sec, 50.171 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 660203, 53, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.23, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 47, 717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2105) [M::mem_pestat] mean and std.dev: (331.41, 581.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2799) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.880 CPU sec, 63.880 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659754, 55, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.23, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 48, 1183) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3511) [M::mem_pestat] mean and std.dev: (311.72, 667.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4675) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.920 CPU sec, 49.923 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 659969, 45, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (348, 1138, 2934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8106) [M::mem_pestat] mean and std.dev: (1786.25, 1899.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10692) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.36, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 50, 568) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1658) [M::mem_pestat] mean and std.dev: (131.65, 290.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2203) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.891 CPU sec, 62.694 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 659796, 46, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (189, 333, 1007) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2643) [M::mem_pestat] mean and std.dev: (506.67, 547.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3461) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.09, 82.46) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 34, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 329) [M::mem_pestat] mean and std.dev: (30.83, 28.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 433) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.931 CPU sec, 50.071 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660383, 54, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.32, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (8, 29, 114) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 326) [M::mem_pestat] mean and std.dev: (38.44, 46.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 432) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1127.049 CPU sec, 63.129 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660184, 63, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.18, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 36, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (39.41, 39.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 408) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.240 CPU sec, 50.016 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660551, 60, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (278, 569, 1547) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4085) [M::mem_pestat] mean and std.dev: (728.10, 557.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5354) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.13, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 39, 897) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2651) [M::mem_pestat] mean and std.dev: (216.63, 442.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3528) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1120.842 CPU sec, 60.729 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 659867, 44, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (317, 513, 955) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2231) [M::mem_pestat] mean and std.dev: (521.90, 350.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2869) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.12, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 35, 496) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1462) [M::mem_pestat] mean and std.dev: (89.23, 223.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1945) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.431 CPU sec, 49.925 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 660105, 42, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.16, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 49, 1146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3400) [M::mem_pestat] mean and std.dev: (404.11, 837.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4527) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1145.436 CPU sec, 66.059 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660678, 42, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.29, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 38, 534) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1562) [M::mem_pestat] mean and std.dev: (208.03, 367.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2076) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1121.980 CPU sec, 49.639 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 660506, 41, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (148, 405, 653) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1663) [M::mem_pestat] mean and std.dev: (425.00, 320.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2168) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.42, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 45, 1180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3508) [M::mem_pestat] mean and std.dev: (567.69, 993.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4672) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1129.277 CPU sec, 63.238 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659986, 44, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.40, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 39, 1057) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3127) [M::mem_pestat] mean and std.dev: (468.16, 809.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4162) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.609 CPU sec, 50.141 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659903, 41, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.11, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 33, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 187) [M::mem_pestat] mean and std.dev: (30.85, 22.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 244) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1141.803 CPU sec, 64.799 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660006, 59, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.12, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 39, 1623) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4827) [M::mem_pestat] mean and std.dev: (718.27, 1279.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6429) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.357 CPU sec, 49.703 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 660396, 53, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (198, 383, 1246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3342) [M::mem_pestat] mean and std.dev: (530.58, 521.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4390) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.23, 82.39) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 34, 97) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 255) [M::mem_pestat] mean and std.dev: (48.00, 53.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 334) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1119.553 CPU sec, 61.109 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660616, 42, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (486, 551, 1682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4074) [M::mem_pestat] mean and std.dev: (856.30, 641.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5270) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.27, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 30, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 190) [M::mem_pestat] mean and std.dev: (29.56, 24.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 249) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.345 CPU sec, 49.666 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660065, 50, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.16, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 43, 1220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3626) [M::mem_pestat] mean and std.dev: (512.76, 981.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4829) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1127.752 CPU sec, 62.747 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660094, 36, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 391, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.02, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 34, 1276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3784) [M::mem_pestat] mean and std.dev: (499.67, 980.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5038) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.825 CPU sec, 49.978 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659931, 48, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.35, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 36, 227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 655) [M::mem_pestat] mean and std.dev: (50.82, 72.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 869) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1143.625 CPU sec, 65.065 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659782, 63, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.08, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 32, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 642) [M::mem_pestat] mean and std.dev: (52.29, 93.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 853) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.605 CPU sec, 50.108 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659895, 42, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.14, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 32, 55) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 133) [M::mem_pestat] mean and std.dev: (28.18, 18.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 172) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1132.916 CPU sec, 62.038 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659974, 38, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.14, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 40, 283) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 813) [M::mem_pestat] mean and std.dev: (88.16, 144.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1078) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.115 CPU sec, 50.021 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660003, 53, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.07, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 48, 1719) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5101) [M::mem_pestat] mean and std.dev: (767.10, 1351.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6792) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.835 CPU sec, 65.037 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 659701, 47, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.22, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 44, 1201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3555) [M::mem_pestat] mean and std.dev: (528.02, 940.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4732) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.677 CPU sec, 49.618 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660313, 50, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (375, 789, 1439) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3567) [M::mem_pestat] mean and std.dev: (862.36, 537.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4631) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.05, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 38, 1627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4851) [M::mem_pestat] mean and std.dev: (846.88, 1440.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6611) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1125.153 CPU sec, 62.948 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660268, 49, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 37, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 433) [M::mem_pestat] mean and std.dev: (42.90, 48.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 571) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.813 CPU sec, 49.842 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659706, 54, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.32, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 56, 1749) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5195) [M::mem_pestat] mean and std.dev: (843.24, 1324.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6918) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1140.584 CPU sec, 65.134 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660025, 58, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.19, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 39, 1051) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3123) [M::mem_pestat] mean and std.dev: (405.08, 775.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4159) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.155 CPU sec, 50.002 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660239, 42, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.26, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 51, 682) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2012) [M::mem_pestat] mean and std.dev: (138.20, 254.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2677) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1143.863 CPU sec, 65.935 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660365, 59, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.30, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 62, 1139) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3379) [M::mem_pestat] mean and std.dev: (434.81, 768.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4499) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1133.700 CPU sec, 49.956 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 660013, 53, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (380, 572, 1048) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2384) [M::mem_pestat] mean and std.dev: (575.82, 340.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3052) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.08, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 52, 228) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 646) [M::mem_pestat] mean and std.dev: (64.29, 75.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 855) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1156.005 CPU sec, 66.169 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659940, 51, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 663, 1150) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2908) [M::mem_pestat] mean and std.dev: (728.80, 482.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3787) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.26, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 35, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (44.87, 46.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 362) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1144.823 CPU sec, 50.612 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 660618, 57, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 418, 803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1913) [M::mem_pestat] mean and std.dev: (473.58, 258.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2468) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.22, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 68, 2608) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7766) [M::mem_pestat] mean and std.dev: (1026.53, 1552.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10345) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1156.189 CPU sec, 66.653 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660034, 51, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.12, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 38, 824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2428) [M::mem_pestat] mean and std.dev: (346.31, 582.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3230) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1144.407 CPU sec, 50.606 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 660010, 50, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.19, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 39, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 171) [M::mem_pestat] mean and std.dev: (36.05, 26.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 222) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1156.902 CPU sec, 68.007 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660425, 40, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (346, 586, 1609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4135) [M::mem_pestat] mean and std.dev: (1034.91, 992.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5398) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.06, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 47, 305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 869) [M::mem_pestat] mean and std.dev: (72.12, 106.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1151) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1151.178 CPU sec, 50.996 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660344, 52, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.23, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 44, 300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 868) [M::mem_pestat] mean and std.dev: (71.38, 134.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1152) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1157.482 CPU sec, 67.238 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660292, 43, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.39, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 39, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 369) [M::mem_pestat] mean and std.dev: (40.47, 37.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 485) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1147.236 CPU sec, 50.887 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659955, 59, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.11, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 27, 476) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1406) [M::mem_pestat] mean and std.dev: (128.47, 300.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1871) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1161.560 CPU sec, 67.240 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 660147, 55, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.36, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 36, 1890) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5636) [M::mem_pestat] mean and std.dev: (699.36, 1237.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7509) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1153.102 CPU sec, 51.048 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 659865, 58, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.23, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 41, 213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 609) [M::mem_pestat] mean and std.dev: (59.30, 96.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 807) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1158.231 CPU sec, 67.556 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660654, 66, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.14, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 35, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (41.60, 46.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1143.545 CPU sec, 50.523 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660117, 42, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (327, 421, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1233) [M::mem_pestat] mean and std.dev: (456.20, 268.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1535) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (395.99, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 40, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 626) [M::mem_pestat] mean and std.dev: (57.09, 86.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 830) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1154.502 CPU sec, 66.531 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659579, 42, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 647, 1846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5042) [M::mem_pestat] mean and std.dev: (885.20, 876.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6640) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.15, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 46, 323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 923) [M::mem_pestat] mean and std.dev: (62.70, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1223) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1138.554 CPU sec, 50.232 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660436, 64, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.21, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 29, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 365) [M::mem_pestat] mean and std.dev: (46.83, 66.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 482) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1149.058 CPU sec, 66.249 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 660192, 45, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (486, 909, 1926) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4806) [M::mem_pestat] mean and std.dev: (1202.08, 917.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6246) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.01, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 25, 89) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 239) [M::mem_pestat] mean and std.dev: (28.36, 35.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 314) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1142.042 CPU sec, 50.492 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660108, 41, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 334, 724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1588) [M::mem_pestat] mean and std.dev: (458.40, 290.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2020) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.13, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 38, 1200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3568) [M::mem_pestat] mean and std.dev: (279.78, 645.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4752) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1152.098 CPU sec, 66.973 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660025, 55, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.21, 82.42) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 42, 853) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2521) [M::mem_pestat] mean and std.dev: (258.08, 486.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3355) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1140.825 CPU sec, 50.428 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660320, 49, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.27, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 45, 1025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3043) [M::mem_pestat] mean and std.dev: (283.40, 642.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4052) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1147.091 CPU sec, 65.621 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660055, 61, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.08, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 48, 1643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4875) [M::mem_pestat] mean and std.dev: (535.48, 1015.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6491) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (394, 773, 1523) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3781) [M::mem_pestat] mean and std.dev: (992.11, 837.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4910) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1135.908 CPU sec, 50.225 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660075, 45, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.22, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 39, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (33.37, 32.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1145.287 CPU sec, 65.449 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 660374, 63, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (376, 828, 1968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5152) [M::mem_pestat] mean and std.dev: (1118.67, 930.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.26, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 63, 1292) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3814) [M::mem_pestat] mean and std.dev: (436.49, 767.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5075) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1136.921 CPU sec, 50.212 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659634, 56, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.12, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 74, 1511) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4495) [M::mem_pestat] mean and std.dev: (760.98, 1301.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5987) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1148.722 CPU sec, 66.402 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660128, 59, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.08, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 41, 1508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4492) [M::mem_pestat] mean and std.dev: (571.85, 1060.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5984) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1138.841 CPU sec, 50.316 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 659927, 51, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (346, 360, 916) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2056) [M::mem_pestat] mean and std.dev: (435.89, 244.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2626) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.19, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 36, 1140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3386) [M::mem_pestat] mean and std.dev: (403.02, 799.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4509) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1148.207 CPU sec, 66.139 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660783, 44, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.20, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 44, 1905) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5683) [M::mem_pestat] mean and std.dev: (799.98, 1565.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7572) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (923, 1074, 2200) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4754) [M::mem_pestat] mean and std.dev: (1185.82, 739.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6031) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1129.772 CPU sec, 50.047 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660014, 61, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.40, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 33, 66) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 168) [M::mem_pestat] mean and std.dev: (30.27, 20.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 219) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1154.775 CPU sec, 67.060 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660638, 59, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.15, 82.42) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 39, 218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 614) [M::mem_pestat] mean and std.dev: (42.11, 46.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 812) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1141.952 CPU sec, 65.027 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 659886, 52, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 461, 2192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5926) [M::mem_pestat] mean and std.dev: (806.08, 830.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7793) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.04, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 51, 318) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (87.38, 140.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1206) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1139.898 CPU sec, 50.346 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 659828, 45, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.32, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 58, 2117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6313) [M::mem_pestat] mean and std.dev: (633.24, 1046.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8411) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1147.253 CPU sec, 64.645 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 659882, 54, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (308, 625, 1772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4700) [M::mem_pestat] mean and std.dev: (849.25, 710.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6164) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.14, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (35, 52, 502) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1436) [M::mem_pestat] mean and std.dev: (146.09, 282.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1903) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1139.792 CPU sec, 50.298 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660892, 48, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 47, 1524) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4536) [M::mem_pestat] mean and std.dev: (607.11, 1135.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6042) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1144.946 CPU sec, 65.841 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 659750, 48, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.35, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 37, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1086) [M::mem_pestat] mean and std.dev: (78.33, 146.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1442) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1143.162 CPU sec, 50.366 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 660365, 36, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 1002, 1093) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2611) [M::mem_pestat] mean and std.dev: (654.38, 355.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3370) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.19, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 43, 1992) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5936) [M::mem_pestat] mean and std.dev: (614.30, 1234.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7908) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (755, 1370, 2028) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4574) [M::mem_pestat] mean and std.dev: (1320.56, 871.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5847) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1148.983 CPU sec, 66.714 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660196, 52, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.26, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 35, 1627) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4847) [M::mem_pestat] mean and std.dev: (647.92, 1181.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6457) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1133.038 CPU sec, 50.071 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660351, 61, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.07, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 51, 1188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3524) [M::mem_pestat] mean and std.dev: (437.22, 842.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4692) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1145.174 CPU sec, 65.878 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660413, 45, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.21, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 71, 1550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4596) [M::mem_pestat] mean and std.dev: (594.67, 1099.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6119) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.735 CPU sec, 49.866 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660668, 44, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.18, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 64, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1420) [M::mem_pestat] mean and std.dev: (173.29, 303.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1885) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1141.912 CPU sec, 64.125 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660058, 45, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.13, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 43, 2410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7190) [M::mem_pestat] mean and std.dev: (790.90, 1525.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9580) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1135.635 CPU sec, 50.164 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659878, 59, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.18, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 46, 943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2787) [M::mem_pestat] mean and std.dev: (372.39, 739.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3709) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1142.839 CPU sec, 65.014 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660177, 51, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.20, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 34, 93) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 247) [M::mem_pestat] mean and std.dev: (41.33, 50.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 324) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1133.898 CPU sec, 50.181 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659534, 45, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.29, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 33, 81) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 213) [M::mem_pestat] mean and std.dev: (29.37, 21.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 279) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.880 CPU sec, 65.969 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660885, 52, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.21, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 41, 100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 274) [M::mem_pestat] mean and std.dev: (36.44, 43.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 361) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1136.601 CPU sec, 50.442 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660270, 50, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 41, 634) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1870) [M::mem_pestat] mean and std.dev: (86.00, 169.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2488) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1145.665 CPU sec, 66.474 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 659707, 34, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 57, 1445) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4289) [M::mem_pestat] mean and std.dev: (625.38, 992.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5711) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1138.353 CPU sec, 50.306 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 659405, 54, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (269, 428, 1354) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3524) [M::mem_pestat] mean and std.dev: (655.07, 569.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4609) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 391, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.12, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 62, 253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 713) [M::mem_pestat] mean and std.dev: (71.33, 104.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 943) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1143.962 CPU sec, 65.333 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 660170, 65, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 431, 824) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1874) [M::mem_pestat] mean and std.dev: (530.25, 319.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2399) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 32, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 480) [M::mem_pestat] mean and std.dev: (38.51, 60.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 636) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.563 CPU sec, 50.000 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660183, 49, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.13, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 40, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 821) [M::mem_pestat] mean and std.dev: (59.10, 118.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1091) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.970 CPU sec, 64.464 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 659953, 54, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.45) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 43, 1497) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4447) [M::mem_pestat] mean and std.dev: (730.92, 1270.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5922) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.013 CPU sec, 49.792 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 659657, 53, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (280, 961, 2619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7297) [M::mem_pestat] mean and std.dev: (1416.00, 1425.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9636) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.09, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 58, 1256) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3714) [M::mem_pestat] mean and std.dev: (463.71, 847.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4943) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1151.381 CPU sec, 66.119 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660532, 48, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.05, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 31, 361) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1045) [M::mem_pestat] mean and std.dev: (60.58, 104.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1387) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1414, 1867, 3335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7177) [M::mem_pestat] mean and std.dev: (2617.00, 1699.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9416) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1125.985 CPU sec, 49.833 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659942, 46, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.15, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 74, 1508) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4474) [M::mem_pestat] mean and std.dev: (817.75, 1214.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5957) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1142.856 CPU sec, 65.655 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 660552, 46, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (395.96, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 46, 220) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (62.05, 85.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 850) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.957 CPU sec, 49.921 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659677, 49, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.13, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 33, 1027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3041) [M::mem_pestat] mean and std.dev: (402.04, 670.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4048) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1148.059 CPU sec, 65.450 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 660527, 51, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 318, 669) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1453) [M::mem_pestat] mean and std.dev: (466.15, 373.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1960) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 57, 1315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3899) [M::mem_pestat] mean and std.dev: (416.19, 739.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5191) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.054 CPU sec, 49.797 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660228, 50, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.28, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 38, 85) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 231) [M::mem_pestat] mean and std.dev: (36.66, 35.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 304) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1141.836 CPU sec, 65.327 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660394, 34, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.37, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 33, 864) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2554) [M::mem_pestat] mean and std.dev: (257.03, 508.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3399) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1136.668 CPU sec, 50.355 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659956, 49, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.22, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 37, 1167) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3469) [M::mem_pestat] mean and std.dev: (354.18, 682.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4620) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1140.179 CPU sec, 66.530 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 660193, 50, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 361, 1360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3624) [M::mem_pestat] mean and std.dev: (458.09, 398.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4756) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 56, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1569) [M::mem_pestat] mean and std.dev: (140.46, 302.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2083) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1136.369 CPU sec, 50.350 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660049, 53, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (395.99, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 55, 1163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3453) [M::mem_pestat] mean and std.dev: (381.41, 793.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4598) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1149.488 CPU sec, 65.078 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 659412, 44, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.32, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 35, 366) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1062) [M::mem_pestat] mean and std.dev: (78.23, 169.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1410) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1143.073 CPU sec, 50.449 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660300, 45, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.06, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 41, 1487) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4429) [M::mem_pestat] mean and std.dev: (694.29, 1273.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5900) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1143.322 CPU sec, 65.894 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 660273, 58, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (331, 407, 1294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3220) [M::mem_pestat] mean and std.dev: (729.62, 724.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4183) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.23, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 49, 229) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (43.89, 44.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 862) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.157 CPU sec, 49.951 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660547, 55, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.35, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 34, 362) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1052) [M::mem_pestat] mean and std.dev: (72.56, 149.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1397) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1141.263 CPU sec, 64.591 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660309, 36, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.16, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 60, 703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2057) [M::mem_pestat] mean and std.dev: (189.23, 363.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2734) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.643 CPU sec, 50.107 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660206, 48, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.24, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 42, 974) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2884) [M::mem_pestat] mean and std.dev: (323.37, 751.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3839) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1146.045 CPU sec, 66.102 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660398, 56, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.03, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 30, 62) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 148) [M::mem_pestat] mean and std.dev: (30.85, 21.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 191) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.342 CPU sec, 49.882 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660404, 51, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.35, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 31, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 474) [M::mem_pestat] mean and std.dev: (51.17, 62.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 625) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (303, 1013, 2593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7173) [M::mem_pestat] mean and std.dev: (1514.60, 1286.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9463) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1143.455 CPU sec, 64.652 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660053, 41, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.10, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 35, 196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 560) [M::mem_pestat] mean and std.dev: (60.09, 82.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 742) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1133.015 CPU sec, 50.121 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660379, 49, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (439, 563, 1567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3823) [M::mem_pestat] mean and std.dev: (984.55, 762.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4951) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.33, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 35, 119) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 321) [M::mem_pestat] mean and std.dev: (43.07, 55.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 422) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.961 CPU sec, 65.918 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659690, 58, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (390, 756, 1161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2703) [M::mem_pestat] mean and std.dev: (810.50, 502.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3474) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.28, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 66, 649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1913) [M::mem_pestat] mean and std.dev: (167.09, 363.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2545) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1133.877 CPU sec, 50.199 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659756, 46, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.20, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 34, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 361) [M::mem_pestat] mean and std.dev: (38.08, 41.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 477) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1143.502 CPU sec, 65.156 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 659533, 46, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (319, 407, 2300) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6262) [M::mem_pestat] mean and std.dev: (960.85, 912.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8243) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.29, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 48, 720) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2132) [M::mem_pestat] mean and std.dev: (231.08, 464.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2838) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1134.375 CPU sec, 50.120 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 660537, 58, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 391, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.10, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 43, 280) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 796) [M::mem_pestat] mean and std.dev: (81.04, 137.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1054) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.314 CPU sec, 64.523 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 660072, 39, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 391, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.07, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 40, 100) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 258) [M::mem_pestat] mean and std.dev: (44.12, 40.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 337) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1136.650 CPU sec, 50.322 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660173, 67, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (313, 343, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1000) [M::mem_pestat] mean and std.dev: (350.10, 177.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1229) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.24, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 56, 1872) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5572) [M::mem_pestat] mean and std.dev: (885.60, 1367.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7422) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.149 CPU sec, 64.936 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660338, 55, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.52, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 32, 421) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1237) [M::mem_pestat] mean and std.dev: (46.67, 73.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1645) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.854 CPU sec, 50.058 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660160, 38, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 39, 238) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (84.31, 149.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 913) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1136.419 CPU sec, 65.201 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 659532, 49, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.26, 82.44) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 39, 285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 823) [M::mem_pestat] mean and std.dev: (77.07, 130.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1092) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1135.026 CPU sec, 50.120 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660264, 44, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.39, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 41, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (42.69, 57.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.147 CPU sec, 65.157 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 659464, 55, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.21, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 48, 1264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3728) [M::mem_pestat] mean and std.dev: (444.13, 820.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4960) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1134.582 CPU sec, 50.229 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 659530, 55, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.20, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 38, 1274) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3784) [M::mem_pestat] mean and std.dev: (429.96, 821.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5039) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1142.582 CPU sec, 66.255 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660491, 44, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.18, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 36, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1101) [M::mem_pestat] mean and std.dev: (98.92, 216.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1462) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.938 CPU sec, 49.906 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 659917, 61, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (216, 251, 716) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1716) [M::mem_pestat] mean and std.dev: (352.80, 268.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2216) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.19, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 33, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 330) [M::mem_pestat] mean and std.dev: (39.86, 43.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 436) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1142.424 CPU sec, 66.819 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660127, 57, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.26, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 50, 1017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3013) [M::mem_pestat] mean and std.dev: (358.63, 712.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4011) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.977 CPU sec, 49.822 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 659962, 45, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.31, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 42, 1127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3349) [M::mem_pestat] mean and std.dev: (329.68, 763.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4460) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1131.935 CPU sec, 64.318 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660398, 49, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.46, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 33, 379) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1115) [M::mem_pestat] mean and std.dev: (66.90, 115.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1483) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.771 CPU sec, 50.049 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660894, 52, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.18, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 58, 1227) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3637) [M::mem_pestat] mean and std.dev: (512.19, 955.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4842) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1134.711 CPU sec, 64.398 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659896, 34, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (340, 721, 2173) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5839) [M::mem_pestat] mean and std.dev: (1339.00, 1327.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7672) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.16, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 29, 59) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 157) [M::mem_pestat] mean and std.dev: (24.81, 20.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 206) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1134.124 CPU sec, 50.185 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659974, 40, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.30, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 39, 1210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3606) [M::mem_pestat] mean and std.dev: (314.74, 644.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4804) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (556, 887, 1562) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3574) [M::mem_pestat] mean and std.dev: (945.30, 582.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4580) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1136.849 CPU sec, 65.156 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660421, 47, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 39, 454) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1326) [M::mem_pestat] mean and std.dev: (131.62, 236.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1762) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.079 CPU sec, 49.584 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659698, 59, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.20, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 57, 803) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2379) [M::mem_pestat] mean and std.dev: (238.10, 496.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3167) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.894 CPU sec, 64.777 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659844, 54, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.21, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 47, 1196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3544) [M::mem_pestat] mean and std.dev: (368.47, 662.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4718) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.363 CPU sec, 49.921 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660023, 59, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.22, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 41, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 944) [M::mem_pestat] mean and std.dev: (86.81, 139.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1251) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.704 CPU sec, 64.262 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 659750, 53, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.25, 82.46) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 35, 69) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 181) [M::mem_pestat] mean and std.dev: (29.30, 22.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 237) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.806 CPU sec, 49.767 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659721, 50, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.36, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 45, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (46.15, 49.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 395) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1129.455 CPU sec, 64.769 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659583, 48, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.15, 82.42) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 32, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 466) [M::mem_pestat] mean and std.dev: (50.40, 79.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 615) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.473 CPU sec, 49.650 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 660263, 46, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 391, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (395.99, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 49, 1506) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4474) [M::mem_pestat] mean and std.dev: (722.91, 1202.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5958) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.842 CPU sec, 66.093 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 660190, 54, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.59, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 29, 1811) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5411) [M::mem_pestat] mean and std.dev: (932.21, 1671.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7619) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1120.020 CPU sec, 49.414 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 660080, 51, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.39, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 32, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 754) [M::mem_pestat] mean and std.dev: (62.95, 113.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 999) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1124.909 CPU sec, 63.275 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660292, 59, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.19, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 34, 385) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1121) [M::mem_pestat] mean and std.dev: (82.25, 188.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1489) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.401 CPU sec, 49.709 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660120, 57, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.24, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 32, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1893) [M::mem_pestat] mean and std.dev: (121.13, 314.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2520) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1126.532 CPU sec, 63.163 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659779, 41, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.11, 82.44) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (8, 28, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1331) [M::mem_pestat] mean and std.dev: (129.46, 297.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1772) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.676 CPU sec, 49.370 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660279, 61, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.18, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 27, 457) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1351) [M::mem_pestat] mean and std.dev: (115.08, 264.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1798) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1124.693 CPU sec, 64.516 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660151, 45, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.10, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 34, 1583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4729) [M::mem_pestat] mean and std.dev: (570.24, 1118.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6302) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.091 CPU sec, 49.861 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660410, 55, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.16, 82.47) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 29, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 318) [M::mem_pestat] mean and std.dev: (37.16, 39.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 418) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1123.915 CPU sec, 65.039 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 660413, 45, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (197, 339, 1413) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3845) [M::mem_pestat] mean and std.dev: (652.50, 691.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.12, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (32, 52, 943) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2765) [M::mem_pestat] mean and std.dev: (301.35, 581.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3676) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1119.815 CPU sec, 49.460 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 660424, 56, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.25, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 51, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1306) [M::mem_pestat] mean and std.dev: (114.70, 219.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1734) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.692 CPU sec, 65.366 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660133, 41, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.14, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 49, 209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 577) [M::mem_pestat] mean and std.dev: (45.91, 40.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 761) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.653 CPU sec, 49.930 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660022, 54, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 391, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (395.99, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 42, 689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2019) [M::mem_pestat] mean and std.dev: (245.00, 526.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2684) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1127.329 CPU sec, 64.799 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660459, 56, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.11, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 50, 989) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2913) [M::mem_pestat] mean and std.dev: (321.33, 607.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3875) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1118.495 CPU sec, 49.375 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 661268, 40, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 394, 712) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1570) [M::mem_pestat] mean and std.dev: (471.00, 356.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1999) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.05, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 34, 1767) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5257) [M::mem_pestat] mean and std.dev: (720.90, 1160.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7002) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1122.587 CPU sec, 64.553 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 659867, 58, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.22, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 33, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 324) [M::mem_pestat] mean and std.dev: (39.09, 40.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 427) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1121.740 CPU sec, 49.608 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660081, 47, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (250, 665, 1323) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3469) [M::mem_pestat] mean and std.dev: (548.33, 417.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.32, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 31, 1209) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3607) [M::mem_pestat] mean and std.dev: (237.92, 656.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4806) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.918 CPU sec, 64.637 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 660273, 32, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (263, 345, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 863) [M::mem_pestat] mean and std.dev: (366.22, 177.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1074) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.18, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 31, 1609) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4805) [M::mem_pestat] mean and std.dev: (588.37, 1115.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6403) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1129.532 CPU sec, 49.891 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 660492, 55, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (289, 611, 1359) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3499) [M::mem_pestat] mean and std.dev: (718.78, 549.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4569) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.20, 82.47) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 32, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 729) [M::mem_pestat] mean and std.dev: (61.44, 103.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 968) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1134.705 CPU sec, 65.977 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660615, 62, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 391, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.03, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 44, 504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1482) [M::mem_pestat] mean and std.dev: (116.50, 239.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1971) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.148 CPU sec, 49.729 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659701, 52, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.03, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 43, 601) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1763) [M::mem_pestat] mean and std.dev: (139.26, 254.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2344) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1140.816 CPU sec, 65.558 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660042, 58, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.13, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 72, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 970) [M::mem_pestat] mean and std.dev: (77.31, 101.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1287) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1134.731 CPU sec, 50.079 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 659110, 46, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (224, 672, 897) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2243) [M::mem_pestat] mean and std.dev: (749.85, 632.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3280) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.36, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 44, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 285) [M::mem_pestat] mean and std.dev: (48.93, 50.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 375) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1140.716 CPU sec, 64.637 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 659637, 48, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.15, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 70, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 910) [M::mem_pestat] mean and std.dev: (97.54, 144.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1205) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.509 CPU sec, 50.042 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 660038, 54, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 479, 931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2339) [M::mem_pestat] mean and std.dev: (624.92, 607.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3056) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.16, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 27, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 206) [M::mem_pestat] mean and std.dev: (26.47, 24.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 271) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1134.882 CPU sec, 64.576 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 660292, 50, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (259, 337, 1027) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2563) [M::mem_pestat] mean and std.dev: (439.83, 322.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3331) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.14, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 54, 1109) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3283) [M::mem_pestat] mean and std.dev: (284.81, 670.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4370) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.089 CPU sec, 49.602 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 659631, 42, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (308, 378, 1614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4226) [M::mem_pestat] mean and std.dev: (773.36, 643.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5532) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 670) [M::mem_pestat] mean and std.dev: (396.24, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (10, 780) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 46, 330) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 952) [M::mem_pestat] mean and std.dev: (85.03, 124.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1263) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (497, 847, 1356) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3074) [M::mem_pestat] mean and std.dev: (698.00, 320.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3933) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.282 CPU sec, 65.582 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659636, 44, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 44, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1332) [M::mem_pestat] mean and std.dev: (96.94, 223.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1770) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1338, 1815, 3951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 9177) [M::mem_pestat] mean and std.dev: (2457.67, 1988.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11790) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1132.313 CPU sec, 50.077 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660181, 64, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.28, 82.42) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 51, 1291) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3839) [M::mem_pestat] mean and std.dev: (419.39, 871.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5113) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1134.734 CPU sec, 65.553 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659356, 56, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 568, 1376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3580) [M::mem_pestat] mean and std.dev: (642.44, 514.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4682) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.08, 82.47) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 51, 2062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6128) [M::mem_pestat] mean and std.dev: (1031.98, 1656.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8161) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1143.854 CPU sec, 65.477 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 660732, 44, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (259, 409, 1193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3061) [M::mem_pestat] mean and std.dev: (529.09, 403.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3995) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.03, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 44, 1163) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3447) [M::mem_pestat] mean and std.dev: (439.07, 740.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4589) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1120.182 CPU sec, 49.386 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660287, 56, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (395, 669, 1182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2756) [M::mem_pestat] mean and std.dev: (833.45, 719.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3710) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.50, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 38, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 360) [M::mem_pestat] mean and std.dev: (43.60, 51.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 475) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.494 CPU sec, 64.356 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 660085, 54, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (337, 539, 1061) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2509) [M::mem_pestat] mean and std.dev: (651.45, 474.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3233) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.26, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 64, 1054) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3126) [M::mem_pestat] mean and std.dev: (404.41, 714.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4162) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.592 CPU sec, 49.825 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659404, 53, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 368, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2961) [M::mem_pestat] mean and std.dev: (575.22, 500.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3863) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.11, 82.46) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 46, 1120) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3318) [M::mem_pestat] mean and std.dev: (486.90, 886.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4417) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.451 CPU sec, 64.365 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 659914, 44, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.21, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 43, 177) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 489) [M::mem_pestat] mean and std.dev: (42.24, 39.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 645) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.596 CPU sec, 49.809 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660731, 59, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.30, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 34, 460) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1344) [M::mem_pestat] mean and std.dev: (117.71, 289.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1786) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1129.473 CPU sec, 63.911 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660141, 52, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (183, 254, 907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2355) [M::mem_pestat] mean and std.dev: (492.10, 558.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3079) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.37, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 45, 198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 574) [M::mem_pestat] mean and std.dev: (55.40, 72.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 762) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.547 CPU sec, 49.982 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660184, 52, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.40, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 41, 559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1645) [M::mem_pestat] mean and std.dev: (120.62, 263.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2188) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1134.037 CPU sec, 65.232 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659713, 55, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.20, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 38, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 905) [M::mem_pestat] mean and std.dev: (48.45, 61.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1200) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.427 CPU sec, 50.050 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659739, 49, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.19, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 34, 1159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3453) [M::mem_pestat] mean and std.dev: (350.89, 698.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4600) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.481 CPU sec, 64.924 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660026, 60, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.36, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 45, 1543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4597) [M::mem_pestat] mean and std.dev: (730.19, 1246.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6124) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.206 CPU sec, 49.820 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 659809, 52, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (320, 799, 1946) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5198) [M::mem_pestat] mean and std.dev: (1009.50, 808.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6824) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.09, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 50, 1907) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5693) [M::mem_pestat] mean and std.dev: (636.94, 1209.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7586) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.998 CPU sec, 65.311 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660073, 55, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.23, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 37, 987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2923) [M::mem_pestat] mean and std.dev: (381.66, 703.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3891) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.850 CPU sec, 49.948 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659955, 51, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.32, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 36, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 426) [M::mem_pestat] mean and std.dev: (42.15, 47.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 562) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1140.202 CPU sec, 65.560 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 660305, 44, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (287, 558, 2332) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6422) [M::mem_pestat] mean and std.dev: (1386.40, 1524.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8467) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.37, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 36, 165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 471) [M::mem_pestat] mean and std.dev: (56.08, 75.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 624) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (431, 1308, 1707) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4259) [M::mem_pestat] mean and std.dev: (1132.00, 734.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5535) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1129.463 CPU sec, 49.826 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 659869, 59, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (619, 997, 1840) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4282) [M::mem_pestat] mean and std.dev: (1096.70, 716.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5503) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.07, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 48, 543) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1609) [M::mem_pestat] mean and std.dev: (161.16, 322.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2142) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1144.216 CPU sec, 66.694 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660803, 45, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.24, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 43, 378) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1100) [M::mem_pestat] mean and std.dev: (131.00, 248.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1461) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.388 CPU sec, 49.780 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660288, 62, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 374, 1202) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3022) [M::mem_pestat] mean and std.dev: (578.55, 454.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3932) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.12, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 38, 1403) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4179) [M::mem_pestat] mean and std.dev: (510.70, 918.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5567) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1134.201 CPU sec, 64.999 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660530, 52, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.34, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 42, 1269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3771) [M::mem_pestat] mean and std.dev: (475.17, 880.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5022) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.513 CPU sec, 49.981 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659824, 45, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (325, 1016, 1475) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3775) [M::mem_pestat] mean and std.dev: (1011.67, 988.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4967) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.26, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 54, 703) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2073) [M::mem_pestat] mean and std.dev: (179.08, 310.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2758) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.890 CPU sec, 64.661 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660681, 42, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.13, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 31, 1588) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4744) [M::mem_pestat] mean and std.dev: (629.05, 1185.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6322) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.564 CPU sec, 49.816 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 659483, 41, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (171, 303, 929) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2445) [M::mem_pestat] mean and std.dev: (714.73, 774.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3812) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.11, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 28, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 414) [M::mem_pestat] mean and std.dev: (34.21, 38.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1142.132 CPU sec, 64.785 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660093, 46, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.48, 82.80) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 51, 691) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2033) [M::mem_pestat] mean and std.dev: (281.43, 518.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2704) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.825 CPU sec, 49.834 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659650, 54, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.18, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 44, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 416) [M::mem_pestat] mean and std.dev: (61.89, 79.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 548) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.816 CPU sec, 65.100 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659914, 49, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (310, 1277, 2246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6118) [M::mem_pestat] mean and std.dev: (1208.50, 1121.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8054) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.12, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 42, 315) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 913) [M::mem_pestat] mean and std.dev: (108.35, 179.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1212) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.698 CPU sec, 49.750 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660270, 48, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.32, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 26, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 371) [M::mem_pestat] mean and std.dev: (33.53, 44.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 491) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1128.925 CPU sec, 64.238 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 660238, 50, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (381, 547, 936) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2046) [M::mem_pestat] mean and std.dev: (575.08, 403.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2601) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.06, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 104, 1878) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5592) [M::mem_pestat] mean and std.dev: (935.33, 1432.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7449) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1120.873 CPU sec, 49.538 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659856, 53, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.34, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 39, 482) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1420) [M::mem_pestat] mean and std.dev: (133.33, 296.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1889) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.901 CPU sec, 65.431 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659797, 49, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (310, 469, 962) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2266) [M::mem_pestat] mean and std.dev: (529.22, 339.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2918) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.34, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 37, 542) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1602) [M::mem_pestat] mean and std.dev: (165.83, 356.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2132) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (356, 1443, 6396) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 18476) [M::mem_pestat] mean and std.dev: (2451.75, 2858.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 24516) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1129.089 CPU sec, 49.896 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660117, 61, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.25, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 54, 1458) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4330) [M::mem_pestat] mean and std.dev: (423.39, 795.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5766) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1146.286 CPU sec, 65.913 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 660188, 48, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (326, 977, 1912) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5084) [M::mem_pestat] mean and std.dev: (1245.71, 1031.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6670) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.22, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 45, 885) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2609) [M::mem_pestat] mean and std.dev: (325.16, 584.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3471) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1138.622 CPU sec, 50.311 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660236, 42, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (465, 631, 844) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1602) [M::mem_pestat] mean and std.dev: (616.40, 271.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1981) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.23, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 36, 264) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 764) [M::mem_pestat] mean and std.dev: (79.29, 126.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1014) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.619 CPU sec, 65.844 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660024, 51, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.18, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 37, 1142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3398) [M::mem_pestat] mean and std.dev: (220.21, 514.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4526) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.609 CPU sec, 50.218 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660304, 47, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.51, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 32, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1449) [M::mem_pestat] mean and std.dev: (87.14, 195.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1927) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1134.214 CPU sec, 65.753 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 659430, 52, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.34, 82.47) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 39, 752) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2232) [M::mem_pestat] mean and std.dev: (179.98, 414.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2972) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.972 CPU sec, 49.957 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659959, 48, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (566, 967, 1968) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4772) [M::mem_pestat] mean and std.dev: (1200.60, 813.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6174) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.17, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 48, 1771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5271) [M::mem_pestat] mean and std.dev: (867.47, 1414.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7021) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1125.803 CPU sec, 65.231 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 660074, 46, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (223, 326, 964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2446) [M::mem_pestat] mean and std.dev: (545.00, 454.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3187) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.18, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 35, 1210) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3600) [M::mem_pestat] mean and std.dev: (556.90, 1002.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4795) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.978 CPU sec, 50.168 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659890, 48, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.40, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 35, 178) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (45.89, 77.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 667) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.929 CPU sec, 64.368 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 660135, 50, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (380, 581, 660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1220) [M::mem_pestat] mean and std.dev: (527.11, 293.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1702) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.31, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 45, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 633) [M::mem_pestat] mean and std.dev: (50.64, 54.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 838) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.892 CPU sec, 49.575 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660194, 44, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.14, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 55, 464) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1352) [M::mem_pestat] mean and std.dev: (111.19, 193.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1796) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.456 CPU sec, 65.223 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660364, 56, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.12, 82.47) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 52, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1367) [M::mem_pestat] mean and std.dev: (113.93, 212.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1814) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (676, 1278, 1436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2956) [M::mem_pestat] mean and std.dev: (1085.73, 635.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3716) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1130.876 CPU sec, 49.964 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659793, 49, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.11, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 41, 342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1002) [M::mem_pestat] mean and std.dev: (70.05, 162.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1332) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.406 CPU sec, 65.538 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 659746, 44, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (602, 1037, 5096) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14084) [M::mem_pestat] mean and std.dev: (2135.62, 2214.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18578) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.21, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 68) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 174) [M::mem_pestat] mean and std.dev: (27.23, 18.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 227) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1115.453 CPU sec, 49.251 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 660927, 55, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (353, 1069, 2030) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5384) [M::mem_pestat] mean and std.dev: (1073.18, 951.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7061) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.09, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 41, 563) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1651) [M::mem_pestat] mean and std.dev: (176.64, 384.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2195) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1119.519 CPU sec, 63.095 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 659758, 35, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.09, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 41, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 429) [M::mem_pestat] mean and std.dev: (56.80, 66.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.567 CPU sec, 49.708 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 659983, 49, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.20, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 40, 1098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3270) [M::mem_pestat] mean and std.dev: (436.32, 764.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4356) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1132.214 CPU sec, 64.027 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659822, 49, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.15, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 36, 329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 963) [M::mem_pestat] mean and std.dev: (57.44, 123.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1280) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.820 CPU sec, 49.687 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 660732, 46, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (174, 586, 1539) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4269) [M::mem_pestat] mean and std.dev: (770.78, 775.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5634) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.14, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 43, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1375) [M::mem_pestat] mean and std.dev: (130.24, 220.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1830) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1127.458 CPU sec, 64.081 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 659952, 42, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (351, 560, 1305) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3213) [M::mem_pestat] mean and std.dev: (678.55, 517.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4167) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 47, 335) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 975) [M::mem_pestat] mean and std.dev: (69.36, 104.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1295) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.247 CPU sec, 49.738 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659956, 53, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.02, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 57, 1004) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2966) [M::mem_pestat] mean and std.dev: (298.93, 587.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3947) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1132.420 CPU sec, 64.963 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 659992, 55, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.05, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 32, 1887) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5635) [M::mem_pestat] mean and std.dev: (916.55, 1543.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7509) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (281, 840, 2187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5999) [M::mem_pestat] mean and std.dev: (1141.60, 1070.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7905) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1125.953 CPU sec, 49.848 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660207, 53, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.20, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 82, 2566) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7666) [M::mem_pestat] mean and std.dev: (974.06, 1587.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10216) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1131.294 CPU sec, 64.476 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 660243, 53, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 398, 1297) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3493) [M::mem_pestat] mean and std.dev: (566.67, 595.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4591) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.08, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 37, 1675) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5001) [M::mem_pestat] mean and std.dev: (819.87, 1421.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6664) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.433 CPU sec, 49.662 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660449, 46, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 48, 760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2248) [M::mem_pestat] mean and std.dev: (247.78, 464.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2992) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1134.635 CPU sec, 65.267 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660194, 59, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.23, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 39, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 344) [M::mem_pestat] mean and std.dev: (40.19, 46.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 455) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1129.881 CPU sec, 49.930 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660177, 50, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.23, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 41, 301) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 877) [M::mem_pestat] mean and std.dev: (52.90, 74.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1165) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1136.013 CPU sec, 65.769 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660442, 53, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 391, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.04, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 44, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1441) [M::mem_pestat] mean and std.dev: (126.84, 246.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1915) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.373 CPU sec, 49.660 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660349, 37, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.24, 82.43) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 39, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1091) [M::mem_pestat] mean and std.dev: (79.32, 125.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1448) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1131.906 CPU sec, 65.271 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660205, 37, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.12, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 46, 406) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1170) [M::mem_pestat] mean and std.dev: (84.97, 193.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1552) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.481 CPU sec, 49.945 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660023, 48, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.18, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 36, 882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2614) [M::mem_pestat] mean and std.dev: (256.05, 612.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3480) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.657 CPU sec, 64.895 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 660219, 43, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.21, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 73, 704) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2080) [M::mem_pestat] mean and std.dev: (220.27, 429.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2768) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.029 CPU sec, 49.582 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 659745, 44, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.11, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 42, 161) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 445) [M::mem_pestat] mean and std.dev: (56.69, 62.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 587) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1131.009 CPU sec, 65.275 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 660381, 40, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (416, 1015, 4098) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11462) [M::mem_pestat] mean and std.dev: (1805.42, 1942.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15144) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.31, 82.81) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 23, 827) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2455) [M::mem_pestat] mean and std.dev: (84.22, 198.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3269) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.865 CPU sec, 49.821 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 660178, 39, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 462, 1263) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3161) [M::mem_pestat] mean and std.dev: (797.14, 686.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4110) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.11, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (8, 28, 63) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 173) [M::mem_pestat] mean and std.dev: (21.20, 17.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 228) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1129.557 CPU sec, 64.571 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 660031, 43, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (308, 525, 1133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2783) [M::mem_pestat] mean and std.dev: (627.09, 503.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3608) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.13, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 41, 1235) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3659) [M::mem_pestat] mean and std.dev: (371.97, 735.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4871) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (330, 943, 2533) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6939) [M::mem_pestat] mean and std.dev: (1448.80, 1286.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9142) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1128.184 CPU sec, 49.951 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659357, 39, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (246, 320, 374) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 630) [M::mem_pestat] mean and std.dev: (318.33, 93.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 758) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.33, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 53, 279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 801) [M::mem_pestat] mean and std.dev: (63.81, 81.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1062) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1136.580 CPU sec, 65.607 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659986, 40, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.22, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 34, 87) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 231) [M::mem_pestat] mean and std.dev: (34.91, 34.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 303) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1137.879 CPU sec, 50.413 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659855, 51, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.13, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 55, 1485) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4421) [M::mem_pestat] mean and std.dev: (666.20, 1018.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5889) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1128.799 CPU sec, 64.361 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660362, 61, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.30, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 40, 1222) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3626) [M::mem_pestat] mean and std.dev: (367.53, 694.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4828) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.992 CPU sec, 49.671 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660012, 61, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.14, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 45, 207) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 583) [M::mem_pestat] mean and std.dev: (42.74, 41.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 771) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1134.525 CPU sec, 63.590 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660182, 42, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.44, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 90, 1249) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3701) [M::mem_pestat] mean and std.dev: (501.38, 930.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4927) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.657 CPU sec, 49.796 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659910, 45, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.44, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 39, 733) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2167) [M::mem_pestat] mean and std.dev: (142.73, 291.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2884) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1119.998 CPU sec, 60.753 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 660417, 45, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (234, 324, 468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 936) [M::mem_pestat] mean and std.dev: (355.67, 198.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1170) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.24, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 54, 1813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5393) [M::mem_pestat] mean and std.dev: (737.31, 1234.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7183) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1118.765 CPU sec, 49.469 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660243, 53, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.18, 82.47) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 50, 724) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2128) [M::mem_pestat] mean and std.dev: (207.71, 485.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2830) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1120.458 CPU sec, 62.281 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659489, 43, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (276, 593, 1196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3036) [M::mem_pestat] mean and std.dev: (789.44, 860.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4233) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.08, 82.46) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 71, 1282) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3788) [M::mem_pestat] mean and std.dev: (555.95, 900.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5041) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.535 CPU sec, 49.614 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 659983, 35, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (284, 357, 686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1490) [M::mem_pestat] mean and std.dev: (444.10, 275.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1892) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.29, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 29, 832) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2474) [M::mem_pestat] mean and std.dev: (247.57, 523.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3295) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1124.576 CPU sec, 63.911 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660338, 48, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.22, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 58, 1942) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5788) [M::mem_pestat] mean and std.dev: (782.57, 1367.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7711) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1119.907 CPU sec, 49.418 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659955, 44, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.22, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (8, 32, 334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 986) [M::mem_pestat] mean and std.dev: (70.06, 144.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1312) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1129.094 CPU sec, 63.581 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659912, 54, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (264, 871, 1083) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2721) [M::mem_pestat] mean and std.dev: (764.00, 566.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3540) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 391, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.05, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 51, 1250) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3714) [M::mem_pestat] mean and std.dev: (312.00, 581.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4946) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1121.204 CPU sec, 49.577 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660024, 43, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (77, 203, 883) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2495) [M::mem_pestat] mean and std.dev: (456.45, 550.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3301) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.42, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 39, 122) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 320) [M::mem_pestat] mean and std.dev: (44.14, 46.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1146.192 CPU sec, 65.699 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660316, 53, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.13, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 44, 375) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1091) [M::mem_pestat] mean and std.dev: (83.30, 157.32) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1449) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.695 CPU sec, 49.814 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 659950, 42, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (760, 1075, 1360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2560) [M::mem_pestat] mean and std.dev: (838.44, 429.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3160) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.14, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 52, 660) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1938) [M::mem_pestat] mean and std.dev: (272.97, 556.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2577) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1132.522 CPU sec, 66.334 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660253, 49, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.02, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 43, 923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2721) [M::mem_pestat] mean and std.dev: (192.61, 397.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3620) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.458 CPU sec, 49.858 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660109, 46, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.25, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 71, 2072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6186) [M::mem_pestat] mean and std.dev: (785.79, 1392.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8243) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.163 CPU sec, 65.428 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 660531, 52, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.15, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 39, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 974) [M::mem_pestat] mean and std.dev: (118.82, 209.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1293) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.263 CPU sec, 50.000 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660378, 42, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.14, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 63, 1244) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3692) [M::mem_pestat] mean and std.dev: (569.95, 1048.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4916) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1143.894 CPU sec, 66.833 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659923, 42, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.18, 82.39) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 41, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 553) [M::mem_pestat] mean and std.dev: (52.15, 57.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 731) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.540 CPU sec, 50.212 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660548, 60, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (367, 663, 1191) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2839) [M::mem_pestat] mean and std.dev: (799.27, 514.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3663) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.22, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 47, 1130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3358) [M::mem_pestat] mean and std.dev: (238.30, 593.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4472) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (623, 1336, 2071) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4967) [M::mem_pestat] mean and std.dev: (1276.33, 1086.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6415) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.602 CPU sec, 64.622 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660252, 53, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.19, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 84, 2834) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8452) [M::mem_pestat] mean and std.dev: (1074.66, 1670.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11261) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.047 CPU sec, 50.100 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 660631, 48, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (142, 315, 467) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1117) [M::mem_pestat] mean and std.dev: (376.40, 321.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1664) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.12, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 41, 775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2265) [M::mem_pestat] mean and std.dev: (258.61, 476.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3010) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.919 CPU sec, 66.528 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659951, 49, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 391, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.09, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 42, 1168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3472) [M::mem_pestat] mean and std.dev: (348.16, 779.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4624) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.628 CPU sec, 49.903 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660015, 52, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.42, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 35, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 428) [M::mem_pestat] mean and std.dev: (40.88, 50.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 566) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.795 CPU sec, 64.792 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660544, 61, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.19, 82.41) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 50, 1009) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2997) [M::mem_pestat] mean and std.dev: (427.21, 792.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3991) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.971 CPU sec, 50.080 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 660304, 45, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (344, 354, 1999) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5309) [M::mem_pestat] mean and std.dev: (973.83, 1002.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6964) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 30, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (34.74, 33.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 559) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1136.586 CPU sec, 65.110 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659726, 57, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.00, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 50, 632) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1854) [M::mem_pestat] mean and std.dev: (191.40, 443.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2465) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1138.288 CPU sec, 50.396 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660188, 58, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.14, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 48, 125) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (47.96, 43.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 425) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1142.644 CPU sec, 66.213 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 660125, 55, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (318, 397, 1014) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2406) [M::mem_pestat] mean and std.dev: (564.00, 522.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3102) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.42, 82.85) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 63, 641) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1879) [M::mem_pestat] mean and std.dev: (213.83, 414.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2498) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.175 CPU sec, 49.887 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 659368, 58, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (299, 525, 2059) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5579) [M::mem_pestat] mean and std.dev: (1077.08, 1011.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.14, 82.43) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 46, 764) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2260) [M::mem_pestat] mean and std.dev: (275.37, 501.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3008) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1139.607 CPU sec, 66.450 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659806, 54, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.24, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 32, 118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 334) [M::mem_pestat] mean and std.dev: (45.56, 67.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 442) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1143.191 CPU sec, 65.540 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 660041, 51, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (919, 1310, 1873) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3781) [M::mem_pestat] mean and std.dev: (1023.20, 505.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4735) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.32, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 50, 565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1671) [M::mem_pestat] mean and std.dev: (210.89, 407.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2224) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1138.882 CPU sec, 50.262 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660136, 60, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.12, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 40, 555) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1635) [M::mem_pestat] mean and std.dev: (121.14, 294.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2175) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.880 CPU sec, 65.671 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660106, 62, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.08, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 33, 224) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 640) [M::mem_pestat] mean and std.dev: (43.37, 57.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 848) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1136.861 CPU sec, 50.359 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660333, 60, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.22, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 50, 294) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 844) [M::mem_pestat] mean and std.dev: (76.83, 133.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1119) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1129.636 CPU sec, 64.223 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 659576, 48, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.02, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 68, 1625) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4841) [M::mem_pestat] mean and std.dev: (716.98, 1355.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6449) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.665 CPU sec, 50.149 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659898, 59, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.12, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 48, 1935) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5771) [M::mem_pestat] mean and std.dev: (787.53, 1342.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7689) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.430 CPU sec, 66.466 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660017, 52, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 290, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1368) [M::mem_pestat] mean and std.dev: (379.80, 276.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1744) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 391, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.09, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 42, 652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1912) [M::mem_pestat] mean and std.dev: (145.02, 360.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2542) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1129.194 CPU sec, 49.961 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660080, 51, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.10, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 43, 465) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1359) [M::mem_pestat] mean and std.dev: (143.00, 302.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1806) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.556 CPU sec, 66.494 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 659695, 48, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (191, 441, 1276) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3446) [M::mem_pestat] mean and std.dev: (585.83, 614.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4531) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.28, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 45, 2307) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6883) [M::mem_pestat] mean and std.dev: (983.89, 1650.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9171) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1129.470 CPU sec, 49.818 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659684, 54, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.22, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 41, 947) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2805) [M::mem_pestat] mean and std.dev: (319.21, 607.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3734) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1134.648 CPU sec, 63.352 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 659807, 50, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (279, 537, 616) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1290) [M::mem_pestat] mean and std.dev: (453.83, 280.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1627) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.13, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 39, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 258) [M::mem_pestat] mean and std.dev: (34.77, 29.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 339) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.522 CPU sec, 49.875 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660044, 48, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.10, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 100, 1689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5023) [M::mem_pestat] mean and std.dev: (490.90, 969.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6690) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1131.170 CPU sec, 64.777 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659434, 60, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.21, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 40, 1203) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3571) [M::mem_pestat] mean and std.dev: (247.64, 602.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4755) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1137.775 CPU sec, 50.290 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660795, 43, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.30, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 31, 455) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1331) [M::mem_pestat] mean and std.dev: (84.26, 202.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1769) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.075 CPU sec, 65.175 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 660427, 43, 10) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 329, 1081) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2713) [M::mem_pestat] mean and std.dev: (474.67, 401.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3529) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.20, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 39, 1559) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4635) [M::mem_pestat] mean and std.dev: (784.83, 1224.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6173) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (344, 1203, 2128) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5696) [M::mem_pestat] mean and std.dev: (1315.20, 1223.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7480) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1125.439 CPU sec, 49.925 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 660488, 52, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 321, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 688) [M::mem_pestat] mean and std.dev: (272.09, 99.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 827) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.11, 82.46) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 35, 147) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (31.63, 34.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 552) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1136.186 CPU sec, 65.224 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 659982, 52, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (230, 395, 882) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2186) [M::mem_pestat] mean and std.dev: (517.60, 417.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2838) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.07, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 35, 166) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 470) [M::mem_pestat] mean and std.dev: (50.21, 80.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 622) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1129.980 CPU sec, 50.007 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659981, 62, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 391, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.07, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 51, 1755) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5227) [M::mem_pestat] mean and std.dev: (860.63, 1449.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6963) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (751, 1119, 1734) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3700) [M::mem_pestat] mean and std.dev: (1186.50, 932.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4915) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1140.750 CPU sec, 67.684 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660224, 46, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.16, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 56, 1953) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5813) [M::mem_pestat] mean and std.dev: (770.19, 1300.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7743) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1129.716 CPU sec, 49.860 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 660326, 57, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (333, 477, 1920) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5094) [M::mem_pestat] mean and std.dev: (742.17, 868.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6681) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.13, 82.43) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 50, 2252) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6718) [M::mem_pestat] mean and std.dev: (955.59, 1563.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8951) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.232 CPU sec, 64.948 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 659889, 51, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.15, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 45, 772) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2278) [M::mem_pestat] mean and std.dev: (136.39, 283.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3031) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.391 CPU sec, 49.904 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660152, 58, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.41, 82.76) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 44, 133) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 357) [M::mem_pestat] mean and std.dev: (45.74, 44.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.084 CPU sec, 64.903 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 660194, 60, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (184, 1047, 2124) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6004) [M::mem_pestat] mean and std.dev: (1355.10, 1395.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7944) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.22, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 49, 1796) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5348) [M::mem_pestat] mean and std.dev: (937.43, 1521.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7124) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1129.723 CPU sec, 49.919 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 660067, 51, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (306, 407, 469) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 795) [M::mem_pestat] mean and std.dev: (346.00, 135.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 958) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.21, 82.42) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 29, 181) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 525) [M::mem_pestat] mean and std.dev: (34.87, 45.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 697) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.885 CPU sec, 65.528 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660045, 63, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.33, 82.46) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 38, 1149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3405) [M::mem_pestat] mean and std.dev: (465.79, 776.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4533) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.261 CPU sec, 49.597 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 660130, 48, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.30, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 47, 617) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1803) [M::mem_pestat] mean and std.dev: (150.27, 324.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2396) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.550 CPU sec, 65.497 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 660333, 56, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (119, 240, 321) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 725) [M::mem_pestat] mean and std.dev: (227.67, 125.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 927) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.13, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 33, 778) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2304) [M::mem_pestat] mean and std.dev: (184.28, 435.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3067) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.294 CPU sec, 49.716 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660285, 55, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.11, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 39, 550) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1622) [M::mem_pestat] mean and std.dev: (124.04, 291.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2158) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.917 CPU sec, 65.861 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 659543, 50, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.33, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 52, 1159) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3443) [M::mem_pestat] mean and std.dev: (414.87, 749.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4585) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (509, 1257, 2504) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6494) [M::mem_pestat] mean and std.dev: (1355.10, 1155.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8489) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1136.618 CPU sec, 50.267 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660110, 50, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.35, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 39, 246) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 704) [M::mem_pestat] mean and std.dev: (68.33, 118.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 933) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1143.640 CPU sec, 67.040 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 660092, 54, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (200, 548, 1058) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2774) [M::mem_pestat] mean and std.dev: (734.90, 742.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3707) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 391, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (395.91, 82.42) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 37, 380) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1110) [M::mem_pestat] mean and std.dev: (87.73, 180.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1475) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.974 CPU sec, 49.677 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659935, 61, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.29, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 45, 1347) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4007) [M::mem_pestat] mean and std.dev: (651.41, 1138.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5337) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.161 CPU sec, 64.176 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660098, 59, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.23, 82.46) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 38, 170) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 484) [M::mem_pestat] mean and std.dev: (37.58, 37.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 641) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1119.334 CPU sec, 49.371 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659831, 39, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.18, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 34, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 303) [M::mem_pestat] mean and std.dev: (45.78, 43.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 396) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.822 CPU sec, 66.307 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 661223, 43, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.27, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 71, 1548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4604) [M::mem_pestat] mean and std.dev: (864.49, 1446.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6649) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (303, 607, 2219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6051) [M::mem_pestat] mean and std.dev: (991.33, 974.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7967) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1124.382 CPU sec, 49.920 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660189, 58, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.18, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 40, 76) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 194) [M::mem_pestat] mean and std.dev: (38.82, 32.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 253) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1144.816 CPU sec, 67.138 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660544, 45, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.42) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 33, 89) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 241) [M::mem_pestat] mean and std.dev: (29.34, 25.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 317) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.470 CPU sec, 49.803 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659670, 58, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.11, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 44, 1279) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3809) [M::mem_pestat] mean and std.dev: (533.66, 901.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5074) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1143.064 CPU sec, 66.758 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659874, 55, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.22, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 93, 1923) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5735) [M::mem_pestat] mean and std.dev: (835.04, 1198.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7641) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.944 CPU sec, 49.869 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659990, 47, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.51, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 34, 822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2430) [M::mem_pestat] mean and std.dev: (263.40, 492.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3234) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.318 CPU sec, 64.632 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660021, 63, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.25, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 37, 490) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1442) [M::mem_pestat] mean and std.dev: (150.64, 300.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1918) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.511 CPU sec, 49.924 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660302, 51, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.19, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 51, 551) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1605) [M::mem_pestat] mean and std.dev: (139.63, 312.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2132) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.516 CPU sec, 65.477 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659611, 34, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (292, 1221, 3969) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11323) [M::mem_pestat] mean and std.dev: (2165.80, 2358.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15000) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.28, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 42, 1233) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3653) [M::mem_pestat] mean and std.dev: (458.74, 798.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4863) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1133.490 CPU sec, 50.256 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659641, 52, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.47, 82.82) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 42, 744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2204) [M::mem_pestat] mean and std.dev: (260.63, 536.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2934) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1142.314 CPU sec, 65.697 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660306, 58, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.28, 82.44) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 31, 112) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 306) [M::mem_pestat] mean and std.dev: (39.02, 39.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 403) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.124 CPU sec, 49.529 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660133, 49, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 42, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1312) [M::mem_pestat] mean and std.dev: (133.55, 264.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1743) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1134.078 CPU sec, 64.821 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 660060, 52, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (240, 337, 548) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1164) [M::mem_pestat] mean and std.dev: (405.62, 286.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1551) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.20, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 39, 1738) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5184) [M::mem_pestat] mean and std.dev: (647.35, 1229.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6907) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.980 CPU sec, 49.959 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 659581, 45, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 334, 1260) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3270) [M::mem_pestat] mean and std.dev: (586.58, 715.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4275) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.23, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 42, 314) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 902) [M::mem_pestat] mean and std.dev: (62.33, 87.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1196) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.875 CPU sec, 64.945 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659671, 39, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.18, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 36, 197) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 559) [M::mem_pestat] mean and std.dev: (50.06, 64.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 740) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.307 CPU sec, 50.081 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659606, 58, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.28, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 44, 266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 762) [M::mem_pestat] mean and std.dev: (59.56, 116.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1010) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1150.185 CPU sec, 68.100 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660574, 49, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.23, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 47, 957) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2839) [M::mem_pestat] mean and std.dev: (190.44, 450.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3780) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (332, 901, 1532) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3932) [M::mem_pestat] mean and std.dev: (901.33, 768.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5132) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1126.691 CPU sec, 49.904 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660139, 42, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.24, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 37, 343) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1001) [M::mem_pestat] mean and std.dev: (41.16, 61.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1330) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (331, 942, 1775) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4663) [M::mem_pestat] mean and std.dev: (1033.80, 681.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6107) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1134.806 CPU sec, 64.689 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660412, 38, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.04, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 42, 186) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 536) [M::mem_pestat] mean and std.dev: (44.33, 62.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 711) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1120.836 CPU sec, 49.667 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 659796, 50, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (305, 857, 1823) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4859) [M::mem_pestat] mean and std.dev: (708.70, 558.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6377) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.20, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 31, 77) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 203) [M::mem_pestat] mean and std.dev: (34.15, 37.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 266) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1134.484 CPU sec, 65.450 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660226, 59, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.18, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 37, 336) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 982) [M::mem_pestat] mean and std.dev: (56.50, 103.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1305) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.755 CPU sec, 49.927 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659475, 42, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.18, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 62, 2285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6815) [M::mem_pestat] mean and std.dev: (928.93, 1477.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9080) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1132.585 CPU sec, 65.909 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660266, 40, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.36, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 40, 650) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1916) [M::mem_pestat] mean and std.dev: (182.00, 391.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2549) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.672 CPU sec, 49.868 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660236, 59, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.20, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 33, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 276) [M::mem_pestat] mean and std.dev: (38.52, 40.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 362) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1134.618 CPU sec, 64.744 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659627, 51, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.05, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 49, 1008) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2968) [M::mem_pestat] mean and std.dev: (404.47, 763.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3948) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.600 CPU sec, 50.047 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660016, 38, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.32, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 36, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 183) [M::mem_pestat] mean and std.dev: (31.70, 27.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 238) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1131.164 CPU sec, 64.171 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 659899, 53, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.16, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 51, 1686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5012) [M::mem_pestat] mean and std.dev: (802.76, 1422.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6675) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.034 CPU sec, 49.845 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660358, 46, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.22, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 59, 1180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3506) [M::mem_pestat] mean and std.dev: (387.73, 684.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4669) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1125.694 CPU sec, 63.948 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660060, 41, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.15, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 26, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1078) [M::mem_pestat] mean and std.dev: (85.97, 155.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1432) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.503 CPU sec, 49.808 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660045, 54, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.30, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 37, 1656) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4928) [M::mem_pestat] mean and std.dev: (737.41, 1256.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6564) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1132.706 CPU sec, 64.641 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 660220, 50, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (187, 315, 1168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3130) [M::mem_pestat] mean and std.dev: (601.15, 628.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4111) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 47, 473) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1369) [M::mem_pestat] mean and std.dev: (134.82, 253.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1817) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1129.194 CPU sec, 49.974 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660406, 50, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.11, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 38, 153) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 425) [M::mem_pestat] mean and std.dev: (39.08, 37.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 561) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1124.101 CPU sec, 64.758 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660539, 40, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.14, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 41, 131) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 355) [M::mem_pestat] mean and std.dev: (43.03, 48.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 467) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.263 CPU sec, 49.642 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 660019, 53, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (426, 469, 1306) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3066) [M::mem_pestat] mean and std.dev: (783.92, 566.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3946) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.35, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 37, 405) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1183) [M::mem_pestat] mean and std.dev: (73.88, 131.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1572) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1132.954 CPU sec, 66.230 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660192, 44, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.05, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 50, 839) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2461) [M::mem_pestat] mean and std.dev: (237.26, 480.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3272) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.732 CPU sec, 49.802 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660232, 46, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.27, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 45, 248) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 718) [M::mem_pestat] mean and std.dev: (56.41, 75.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1131.294 CPU sec, 64.984 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659483, 45, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.27, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 54, 401) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1161) [M::mem_pestat] mean and std.dev: (110.11, 146.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1541) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.993 CPU sec, 50.132 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 660138, 48, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.04, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 38, 1468) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4372) [M::mem_pestat] mean and std.dev: (414.24, 864.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5824) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1128.762 CPU sec, 65.259 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659753, 58, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.47, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 36, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 311) [M::mem_pestat] mean and std.dev: (35.91, 40.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 409) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.095 CPU sec, 49.823 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659822, 50, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.09, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 46, 320) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 924) [M::mem_pestat] mean and std.dev: (93.27, 164.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1226) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1136.376 CPU sec, 65.368 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659585, 51, 13) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (406, 579, 2180) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5728) [M::mem_pestat] mean and std.dev: (1078.90, 839.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7502) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.15, 82.42) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 42, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (48.40, 54.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (621, 1032, 1545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3393) [M::mem_pestat] mean and std.dev: (1091.92, 646.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4317) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1122.574 CPU sec, 49.564 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 660387, 42, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.10, 82.39) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 54, 463) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1353) [M::mem_pestat] mean and std.dev: (128.26, 256.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1798) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1122.552 CPU sec, 63.216 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659549, 39, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.06, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 47, 649) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1895) [M::mem_pestat] mean and std.dev: (228.82, 421.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2518) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.765 CPU sec, 49.922 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 659818, 66, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.13, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 42, 188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 524) [M::mem_pestat] mean and std.dev: (44.22, 59.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 692) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.301 CPU sec, 64.589 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660075, 48, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (395.94, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 41, 129) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 341) [M::mem_pestat] mean and std.dev: (42.84, 35.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 447) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.624 CPU sec, 49.803 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 659762, 52, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 391, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.04, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 41, 152) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 424) [M::mem_pestat] mean and std.dev: (48.57, 64.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 560) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1134.889 CPU sec, 64.582 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660169, 55, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.43, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 52, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259) [M::mem_pestat] mean and std.dev: (48.22, 35.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 336) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1129.357 CPU sec, 49.837 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 659938, 43, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (255, 658, 1389) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3657) [M::mem_pestat] mean and std.dev: (636.55, 479.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4791) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.25, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 47, 288) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 810) [M::mem_pestat] mean and std.dev: (80.26, 92.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1071) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1125.125 CPU sec, 61.136 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 659729, 60, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (290, 916, 1285) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3275) [M::mem_pestat] mean and std.dev: (771.10, 549.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4270) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.23, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 52, 950) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2826) [M::mem_pestat] mean and std.dev: (282.40, 618.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3764) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.489 CPU sec, 49.336 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659788, 57, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.40, 82.83) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 25, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 411) [M::mem_pestat] mean and std.dev: (40.51, 59.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 544) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1123.303 CPU sec, 60.478 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659603, 56, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 40, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 260) [M::mem_pestat] mean and std.dev: (32.76, 27.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.729 CPU sec, 50.040 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 659926, 40, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (274, 471, 1062) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2638) [M::mem_pestat] mean and std.dev: (646.00, 540.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3426) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.21, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 38, 296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 854) [M::mem_pestat] mean and std.dev: (91.47, 166.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1133) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1122.533 CPU sec, 61.772 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659568, 62, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (265, 1117, 1308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3394) [M::mem_pestat] mean and std.dev: (720.78, 533.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4437) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.21, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 45, 1115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3309) [M::mem_pestat] mean and std.dev: (366.91, 732.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4406) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.591 CPU sec, 49.900 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 660483, 43, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (248, 436, 717) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1655) [M::mem_pestat] mean and std.dev: (475.31, 292.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2124) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.21, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 53, 610) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1800) [M::mem_pestat] mean and std.dev: (109.15, 255.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2395) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1121.869 CPU sec, 61.878 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659841, 59, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.45, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 40, 869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2573) [M::mem_pestat] mean and std.dev: (174.60, 363.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3425) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1118.377 CPU sec, 49.444 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660363, 38, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (424, 807, 945) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1987) [M::mem_pestat] mean and std.dev: (609.10, 315.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2508) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.04, 82.45) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 102, 2517) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7515) [M::mem_pestat] mean and std.dev: (1117.58, 1641.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10014) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.881 CPU sec, 63.960 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660127, 58, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.21, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 36, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 181) [M::mem_pestat] mean and std.dev: (32.24, 21.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 235) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.423 CPU sec, 50.088 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660340, 56, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.15, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 38, 527) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1551) [M::mem_pestat] mean and std.dev: (154.89, 343.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2063) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1125.387 CPU sec, 62.436 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 659675, 50, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (447, 1228, 3270) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8916) [M::mem_pestat] mean and std.dev: (2490.45, 2917.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 14159) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.27, 82.77) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 29, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 196) [M::mem_pestat] mean and std.dev: (29.15, 27.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 258) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1121.568 CPU sec, 49.570 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659576, 45, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (429, 649, 1063) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2331) [M::mem_pestat] mean and std.dev: (720.78, 467.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2965) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.13, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 33, 910) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2700) [M::mem_pestat] mean and std.dev: (212.82, 516.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3595) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1125.773 CPU sec, 63.600 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659515, 54, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.14, 82.39) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 55, 1214) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3614) [M::mem_pestat] mean and std.dev: (413.23, 817.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4814) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1129.947 CPU sec, 50.005 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 659614, 46, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.22, 82.39) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 28, 1196) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3560) [M::mem_pestat] mean and std.dev: (573.52, 1028.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4742) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1120.567 CPU sec, 61.336 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659973, 50, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.26, 82.44) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 42, 1234) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3654) [M::mem_pestat] mean and std.dev: (435.64, 928.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4864) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1120.235 CPU sec, 49.539 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660176, 59, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.39, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 37, 255) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 721) [M::mem_pestat] mean and std.dev: (69.38, 94.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 954) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.342 CPU sec, 62.123 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660567, 45, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.40, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 30, 102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 280) [M::mem_pestat] mean and std.dev: (35.19, 39.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 369) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1142.047 CPU sec, 64.396 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 659781, 50, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (825, 1129, 1661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3333) [M::mem_pestat] mean and std.dev: (1012.60, 494.24) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4169) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.25, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 37, 126) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 342) [M::mem_pestat] mean and std.dev: (52.70, 63.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 450) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.721 CPU sec, 49.782 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 660009, 51, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (214, 446, 1158) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3046) [M::mem_pestat] mean and std.dev: (696.25, 588.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3990) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.46) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 44, 1714) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5102) [M::mem_pestat] mean and std.dev: (728.62, 1226.59) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6796) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.692 CPU sec, 65.130 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659998, 59, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.23, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 37, 53) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 133) [M::mem_pestat] mean and std.dev: (28.98, 22.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 173) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.415 CPU sec, 49.655 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660053, 53, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.21, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 49, 363) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1053) [M::mem_pestat] mean and std.dev: (74.79, 97.47) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1398) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1124.483 CPU sec, 62.814 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660101, 48, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.08, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 55, 319) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (77.61, 134.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1201) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.322 CPU sec, 49.738 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (16, 659557, 60, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (364, 932, 1592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4048) [M::mem_pestat] mean and std.dev: (968.19, 694.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5276) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.23, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 38, 915) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2705) [M::mem_pestat] mean and std.dev: (373.85, 693.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3600) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1126.434 CPU sec, 63.593 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660322, 53, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.10, 82.45) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 38, 145) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 405) [M::mem_pestat] mean and std.dev: (43.73, 46.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 535) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.712 CPU sec, 49.689 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659674, 52, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.23, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 44, 804) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2376) [M::mem_pestat] mean and std.dev: (252.42, 556.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3162) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1123.331 CPU sec, 62.006 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660285, 54, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.27, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 44, 132) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 364) [M::mem_pestat] mean and std.dev: (39.60, 35.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 480) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1120.006 CPU sec, 49.533 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 660074, 51, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (219, 567, 1388) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3726) [M::mem_pestat] mean and std.dev: (846.71, 745.19) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4895) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.12, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 55, 1192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3522) [M::mem_pestat] mean and std.dev: (472.40, 873.64) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4687) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.823 CPU sec, 64.893 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660111, 49, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.09, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 39, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 307) [M::mem_pestat] mean and std.dev: (39.42, 40.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 404) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.682 CPU sec, 50.166 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 659651, 47, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.22, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 52, 1723) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5115) [M::mem_pestat] mean and std.dev: (854.49, 1333.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6811) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (406, 1268, 1652) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4144) [M::mem_pestat] mean and std.dev: (971.78, 637.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5390) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.744 CPU sec, 64.892 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660205, 56, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 794, 2407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6617) [M::mem_pestat] mean and std.dev: (1437.82, 1676.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8722) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.27, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 47, 1102) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3272) [M::mem_pestat] mean and std.dev: (363.39, 684.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4357) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.689 CPU sec, 49.855 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 660028, 54, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.37, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 44, 154) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (48.02, 52.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 574) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1131.412 CPU sec, 63.772 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 659738, 47, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (336, 351, 829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1815) [M::mem_pestat] mean and std.dev: (371.56, 225.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2308) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.14, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 36, 130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 356) [M::mem_pestat] mean and std.dev: (42.37, 40.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 469) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.363 CPU sec, 49.856 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660410, 52, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.20, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 50, 308) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 890) [M::mem_pestat] mean and std.dev: (65.85, 123.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1181) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1125.342 CPU sec, 63.548 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660359, 49, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 44, 643) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1897) [M::mem_pestat] mean and std.dev: (167.22, 353.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2524) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.362 CPU sec, 49.752 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659707, 48, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (302, 1824, 4022) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 11462) [M::mem_pestat] mean and std.dev: (2391.10, 2665.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 15182) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.15, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 33, 756) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2230) [M::mem_pestat] mean and std.dev: (127.44, 316.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2967) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1129.936 CPU sec, 63.383 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 659692, 45, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (349, 1096, 4925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 14077) [M::mem_pestat] mean and std.dev: (1878.58, 2039.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 18653) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.25, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 31, 80) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 218) [M::mem_pestat] mean and std.dev: (37.18, 43.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 287) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1118.792 CPU sec, 49.486 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660355, 56, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 391, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (395.96, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 51, 1127) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3333) [M::mem_pestat] mean and std.dev: (322.00, 537.74) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4436) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1132.793 CPU sec, 64.485 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 659826, 54, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (407, 481, 760) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1466) [M::mem_pestat] mean and std.dev: (526.82, 299.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1819) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.24, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 51, 1668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4952) [M::mem_pestat] mean and std.dev: (584.90, 1048.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6594) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (311, 932, 1876) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5006) [M::mem_pestat] mean and std.dev: (1224.27, 1142.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6571) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1129.910 CPU sec, 50.111 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660015, 46, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (315, 350, 420) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (105, 630) [M::mem_pestat] mean and std.dev: (318.30, 104.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 736) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.21, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 31, 912) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2712) [M::mem_pestat] mean and std.dev: (253.28, 583.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3612) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.154 CPU sec, 64.029 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659764, 47, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.20, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 52, 479) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1395) [M::mem_pestat] mean and std.dev: (163.15, 293.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1853) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1136.092 CPU sec, 50.218 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660899, 62, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.24, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 35, 113) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 317) [M::mem_pestat] mean and std.dev: (36.02, 42.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 419) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1123.651 CPU sec, 63.038 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659841, 46, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (334, 797, 1513) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3871) [M::mem_pestat] mean and std.dev: (1114.30, 1055.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5337) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.03, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 38, 108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 288) [M::mem_pestat] mean and std.dev: (41.36, 38.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.003 CPU sec, 49.806 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660240, 55, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.48, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 34, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 275) [M::mem_pestat] mean and std.dev: (43.46, 47.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1124.916 CPU sec, 63.655 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660255, 62, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (448, 1143, 1661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4087) [M::mem_pestat] mean and std.dev: (1040.10, 852.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5300) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.28, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 34, 172) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 492) [M::mem_pestat] mean and std.dev: (49.41, 87.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 652) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.044 CPU sec, 49.715 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660515, 64, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.08, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 54, 590) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1728) [M::mem_pestat] mean and std.dev: (100.48, 227.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2297) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1126.929 CPU sec, 64.502 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659650, 55, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.18, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 38, 1035) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3071) [M::mem_pestat] mean and std.dev: (385.54, 678.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4089) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.265 CPU sec, 49.773 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 659491, 48, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (354, 674, 1680) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4332) [M::mem_pestat] mean and std.dev: (950.17, 950.92) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5658) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.15, 82.45) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 45, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3417) [M::mem_pestat] mean and std.dev: (401.40, 782.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4547) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.106 CPU sec, 65.739 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660070, 49, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.04, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 30, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 468) [M::mem_pestat] mean and std.dev: (32.55, 33.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 618) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.484 CPU sec, 50.081 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 660183, 50, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (360, 1001, 2017) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5331) [M::mem_pestat] mean and std.dev: (1019.42, 930.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6988) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.45, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 44, 1708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5088) [M::mem_pestat] mean and std.dev: (931.92, 1458.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6778) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.526 CPU sec, 65.078 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660532, 47, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.36, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 34, 251) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 719) [M::mem_pestat] mean and std.dev: (39.19, 51.42) [M::mem_pestat] low and high boundaries for proper pairs: (1, 953) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.378 CPU sec, 50.004 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660286, 61, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 670) [M::mem_pestat] mean and std.dev: (396.21, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (10, 780) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 38, 1231) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3663) [M::mem_pestat] mean and std.dev: (405.17, 859.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4879) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.398 CPU sec, 65.502 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660031, 57, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.19, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 47, 410) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1184) [M::mem_pestat] mean and std.dev: (101.79, 190.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1571) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1120.951 CPU sec, 49.565 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660155, 43, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.28, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 26, 83) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 221) [M::mem_pestat] mean and std.dev: (25.15, 19.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 290) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.058 CPU sec, 66.298 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660561, 57, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (386, 724, 1597) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4019) [M::mem_pestat] mean and std.dev: (918.55, 733.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5230) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.05, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 38, 1742) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5192) [M::mem_pestat] mean and std.dev: (646.13, 1202.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6917) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1135.155 CPU sec, 50.152 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660207, 61, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.30, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 38, 447) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1303) [M::mem_pestat] mean and std.dev: (169.57, 325.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1731) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.907 CPU sec, 65.340 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 660767, 55, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (294, 1210, 2573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7131) [M::mem_pestat] mean and std.dev: (1300.00, 1425.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9410) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.10, 82.46) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 53, 1086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3212) [M::mem_pestat] mean and std.dev: (551.00, 941.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4319) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1117.731 CPU sec, 49.468 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659564, 48, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.05, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 39, 1668) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4980) [M::mem_pestat] mean and std.dev: (792.78, 1448.15) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6636) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1142.532 CPU sec, 65.779 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 660154, 52, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (395.90, 82.41) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 40, 1143) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3401) [M::mem_pestat] mean and std.dev: (343.16, 736.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4530) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.057 CPU sec, 50.051 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 659914, 55, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.04, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 48, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 301) [M::mem_pestat] mean and std.dev: (44.11, 36.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 394) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1131.112 CPU sec, 63.678 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 659697, 49, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (370, 787, 1086) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2518) [M::mem_pestat] mean and std.dev: (651.09, 373.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3234) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.26, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 41, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1025) [M::mem_pestat] mean and std.dev: (66.13, 121.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1361) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.473 CPU sec, 50.112 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660224, 51, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.26, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 28, 149) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 417) [M::mem_pestat] mean and std.dev: (43.80, 67.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 551) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.991 CPU sec, 66.310 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659871, 53, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (284, 462, 2187) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5993) [M::mem_pestat] mean and std.dev: (1087.20, 1197.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7896) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.14, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 69, 1329) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3947) [M::mem_pestat] mean and std.dev: (446.00, 767.49) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5256) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.132 CPU sec, 50.044 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 660165, 51, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (338, 498, 1025) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2399) [M::mem_pestat] mean and std.dev: (687.92, 579.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3086) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.09, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 41, 567) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1665) [M::mem_pestat] mean and std.dev: (189.86, 369.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2214) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.422 CPU sec, 66.254 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 659779, 38, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (271, 323, 852) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2014) [M::mem_pestat] mean and std.dev: (547.64, 388.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2595) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.11, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 63, 826) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2442) [M::mem_pestat] mean and std.dev: (262.66, 483.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3250) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.634 CPU sec, 50.160 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 660028, 45, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (366, 749, 2045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5403) [M::mem_pestat] mean and std.dev: (953.56, 795.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7082) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.48, 82.75) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (9, 25, 137) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 393) [M::mem_pestat] mean and std.dev: (35.89, 58.84) [M::mem_pestat] low and high boundaries for proper pairs: (1, 521) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1131.299 CPU sec, 65.035 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 660102, 43, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 391, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.05, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 52, 98) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 264) [M::mem_pestat] mean and std.dev: (45.80, 47.93) [M::mem_pestat] low and high boundaries for proper pairs: (1, 347) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.246 CPU sec, 50.077 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660134, 51, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.14, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 28, 108) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 292) [M::mem_pestat] mean and std.dev: (34.02, 32.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 384) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1143.243 CPU sec, 68.558 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 659890, 40, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (281, 359, 1360) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3518) [M::mem_pestat] mean and std.dev: (600.93, 559.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4597) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.20, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 47, 1269) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3779) [M::mem_pestat] mean and std.dev: (376.57, 796.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5034) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.067 CPU sec, 50.001 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660602, 41, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.13, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 61, 1921) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5729) [M::mem_pestat] mean and std.dev: (650.47, 1158.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7633) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.629 CPU sec, 66.109 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 660067, 54, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (395.90, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 41, 353) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1029) [M::mem_pestat] mean and std.dev: (69.60, 115.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1367) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.965 CPU sec, 49.829 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 659905, 52, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (277, 316, 1266) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3244) [M::mem_pestat] mean and std.dev: (543.00, 521.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4233) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.22, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 48, 182) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 504) [M::mem_pestat] mean and std.dev: (54.54, 81.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 665) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1132.015 CPU sec, 65.418 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659463, 48, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.36, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 79, 1589) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4715) [M::mem_pestat] mean and std.dev: (624.45, 1125.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6278) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (307, 1147, 2979) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8323) [M::mem_pestat] mean and std.dev: (1367.73, 1221.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10995) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1129.544 CPU sec, 49.768 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660237, 44, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (377, 409, 1156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2714) [M::mem_pestat] mean and std.dev: (663.73, 521.08) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3493) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.15, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 44, 412) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1210) [M::mem_pestat] mean and std.dev: (115.05, 237.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1609) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.883 CPU sec, 65.434 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660422, 52, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (297, 319, 1162) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2892) [M::mem_pestat] mean and std.dev: (380.33, 300.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3757) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.04, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 36, 869) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2565) [M::mem_pestat] mean and std.dev: (259.18, 550.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3413) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.385 CPU sec, 49.818 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659929, 36, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.57, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 73, 2573) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7667) [M::mem_pestat] mean and std.dev: (1071.80, 1636.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10214) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (751, 801, 2369) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5605) [M::mem_pestat] mean and std.dev: (1584.82, 1424.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7283) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1141.778 CPU sec, 66.271 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660300, 38, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (228, 361, 1219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3201) [M::mem_pestat] mean and std.dev: (629.73, 562.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4192) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.20, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 100, 1934) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5744) [M::mem_pestat] mean and std.dev: (952.25, 1394.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7649) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.146 CPU sec, 49.769 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660799, 56, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.22, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 38, 1961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5847) [M::mem_pestat] mean and std.dev: (846.54, 1518.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7790) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.867 CPU sec, 66.551 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 660038, 48, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.22, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 77, 1964) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5838) [M::mem_pestat] mean and std.dev: (845.75, 1346.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7775) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.035 CPU sec, 49.754 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660457, 54, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.40, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 40, 117) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 325) [M::mem_pestat] mean and std.dev: (39.12, 48.68) [M::mem_pestat] low and high boundaries for proper pairs: (1, 429) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1129.069 CPU sec, 63.446 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660225, 57, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.11, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 58, 614) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1798) [M::mem_pestat] mean and std.dev: (143.33, 289.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2390) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.308 CPU sec, 49.616 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 660231, 59, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (303, 1260, 2284) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6246) [M::mem_pestat] mean and std.dev: (1283.70, 1084.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8227) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.09, 82.56) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 35, 1342) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3990) [M::mem_pestat] mean and std.dev: (370.73, 811.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5314) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1122.113 CPU sec, 62.842 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659987, 53, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.28, 82.46) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 37, 99) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 273) [M::mem_pestat] mean and std.dev: (30.68, 24.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 360) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1121.326 CPU sec, 49.613 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659602, 43, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 90, 1912) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5694) [M::mem_pestat] mean and std.dev: (729.20, 1192.18) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7585) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1118.930 CPU sec, 62.574 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660166, 50, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 670) [M::mem_pestat] mean and std.dev: (396.36, 82.91) [M::mem_pestat] low and high boundaries for proper pairs: (10, 780) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 50, 1986) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5906) [M::mem_pestat] mean and std.dev: (1010.08, 1545.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7866) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1118.126 CPU sec, 49.399 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660072, 50, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.21, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 45, 1434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4260) [M::mem_pestat] mean and std.dev: (464.16, 867.80) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5673) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1117.797 CPU sec, 61.586 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 659584, 58, 12) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (304, 822, 1666) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4390) [M::mem_pestat] mean and std.dev: (835.70, 715.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5752) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.28, 82.74) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 43, 298) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 862) [M::mem_pestat] mean and std.dev: (67.33, 107.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1144) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (492, 726, 1072) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2232) [M::mem_pestat] mean and std.dev: (743.08, 377.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2812) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1120.826 CPU sec, 49.650 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660402, 48, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.27, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 41, 2268) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6774) [M::mem_pestat] mean and std.dev: (633.61, 1280.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9027) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (379, 720, 1296) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3130) [M::mem_pestat] mean and std.dev: (784.20, 470.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4047) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1117.823 CPU sec, 60.758 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 659499, 49, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.20, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 54, 1136) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3362) [M::mem_pestat] mean and std.dev: (407.84, 760.89) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4475) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.900 CPU sec, 49.800 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660378, 56, 11) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.24, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (29, 60, 1066) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3140) [M::mem_pestat] mean and std.dev: (372.64, 730.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4177) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (1034, 1258, 1838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3446) [M::mem_pestat] mean and std.dev: (1153.00, 451.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4250) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1124.776 CPU sec, 61.115 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659861, 54, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (178, 322, 629) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1531) [M::mem_pestat] mean and std.dev: (274.25, 173.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1982) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.39, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 33, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 388) [M::mem_pestat] mean and std.dev: (49.78, 71.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 512) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.893 CPU sec, 49.604 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 660225, 53, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.19, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (34, 53, 325) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 907) [M::mem_pestat] mean and std.dev: (86.48, 102.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1198) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1116.152 CPU sec, 62.119 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660351, 53, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.09, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 68, 1874) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5584) [M::mem_pestat] mean and std.dev: (930.72, 1500.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7439) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1114.390 CPU sec, 49.224 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 660353, 45, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 391, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (395.95, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 34, 1011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3005) [M::mem_pestat] mean and std.dev: (214.21, 546.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4002) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1122.099 CPU sec, 63.281 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660266, 47, 1) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (351, 762, 2541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6921) [M::mem_pestat] mean and std.dev: (1347.90, 1755.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9111) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.08, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 50, 1045) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3089) [M::mem_pestat] mean and std.dev: (438.36, 836.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4111) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1119.744 CPU sec, 49.588 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 659962, 45, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (407, 909, 1776) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4514) [M::mem_pestat] mean and std.dev: (1124.58, 984.40) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5883) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.07, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (30, 57, 1218) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3594) [M::mem_pestat] mean and std.dev: (561.42, 926.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4782) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1122.910 CPU sec, 62.860 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660383, 53, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.12, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 39, 370) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1058) [M::mem_pestat] mean and std.dev: (80.07, 128.50) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1402) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (436, 956, 2576) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6856) [M::mem_pestat] mean and std.dev: (1520.70, 1473.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8996) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1121.724 CPU sec, 49.625 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659735, 38, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.36, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 43, 639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1869) [M::mem_pestat] mean and std.dev: (121.43, 336.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2484) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1136.762 CPU sec, 64.805 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 660302, 47, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (205, 369, 1639) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4507) [M::mem_pestat] mean and std.dev: (853.42, 1074.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5941) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.12, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 38, 95) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 259) [M::mem_pestat] mean and std.dev: (32.27, 26.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 341) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1134.027 CPU sec, 50.153 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660540, 50, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.16, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 34, 171) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 479) [M::mem_pestat] mean and std.dev: (39.24, 41.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 633) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1129.790 CPU sec, 64.065 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660034, 55, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.50, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 29, 155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 437) [M::mem_pestat] mean and std.dev: (44.59, 71.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 578) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.752 CPU sec, 49.947 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659888, 54, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.16, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 38, 456) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1340) [M::mem_pestat] mean and std.dev: (50.44, 90.44) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1782) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1125.548 CPU sec, 64.224 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660326, 48, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 37, 667) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1963) [M::mem_pestat] mean and std.dev: (168.32, 400.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2611) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1135.730 CPU sec, 50.296 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660015, 53, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.15, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 40, 603) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1779) [M::mem_pestat] mean and std.dev: (168.29, 368.20) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2367) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.243 CPU sec, 65.153 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 660082, 50, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.40, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 52, 771) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2277) [M::mem_pestat] mean and std.dev: (196.25, 441.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3030) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.535 CPU sec, 49.837 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659944, 48, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.19, 82.54) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 31, 106) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 284) [M::mem_pestat] mean and std.dev: (31.68, 24.90) [M::mem_pestat] low and high boundaries for proper pairs: (1, 373) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1122.876 CPU sec, 64.733 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 659713, 43, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 324, 648) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1316) [M::mem_pestat] mean and std.dev: (379.60, 224.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1650) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.30, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 24, 61) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 159) [M::mem_pestat] mean and std.dev: (23.46, 22.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 208) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1117.356 CPU sec, 49.493 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 659933, 54, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (295, 333, 991) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2383) [M::mem_pestat] mean and std.dev: (593.17, 440.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3079) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.25, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 47, 951) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2805) [M::mem_pestat] mean and std.dev: (303.62, 616.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3732) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.568 CPU sec, 64.283 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659727, 43, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.40, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 37, 82) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 210) [M::mem_pestat] mean and std.dev: (41.35, 41.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 274) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.282 CPU sec, 50.430 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659950, 52, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.18, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 50, 1165) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3455) [M::mem_pestat] mean and std.dev: (367.02, 665.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4600) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1144.895 CPU sec, 67.170 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 659699, 59, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (227, 509, 1838) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5060) [M::mem_pestat] mean and std.dev: (802.10, 737.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6671) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.06, 82.45) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 34, 377) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1107) [M::mem_pestat] mean and std.dev: (92.20, 178.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1472) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.789 CPU sec, 49.716 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 660176, 45, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.14, 82.46) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 31, 201) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 571) [M::mem_pestat] mean and std.dev: (43.58, 51.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 756) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.052 CPU sec, 65.947 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 659983, 55, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.30, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 39, 157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 443) [M::mem_pestat] mean and std.dev: (48.48, 57.38) [M::mem_pestat] low and high boundaries for proper pairs: (1, 586) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.456 CPU sec, 49.728 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660178, 56, 13) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.48, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 39, 972) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2888) [M::mem_pestat] mean and std.dev: (241.71, 497.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3846) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (394, 777, 2793) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7591) [M::mem_pestat] mean and std.dev: (1881.31, 2242.13) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10850) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.112 CPU sec, 65.440 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659745, 49, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (253, 794, 1470) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3904) [M::mem_pestat] mean and std.dev: (725.89, 576.57) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5121) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.13, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 44, 1192) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3526) [M::mem_pestat] mean and std.dev: (434.23, 831.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4693) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1129.093 CPU sec, 50.031 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 660088, 51, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 327, 1011) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2617) [M::mem_pestat] mean and std.dev: (564.40, 481.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3420) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.16, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 45, 1213) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3607) [M::mem_pestat] mean and std.dev: (517.84, 903.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4804) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1139.199 CPU sec, 66.087 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 659925, 54, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (368, 770, 2036) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5372) [M::mem_pestat] mean and std.dev: (1075.57, 1008.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7040) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.13, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 42, 747) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2209) [M::mem_pestat] mean and std.dev: (285.51, 598.75) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2940) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.095 CPU sec, 65.394 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660143, 45, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 47, 541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1591) [M::mem_pestat] mean and std.dev: (122.51, 196.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2116) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1129.442 CPU sec, 49.970 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 659650, 45, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.44, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 45, 1732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5162) [M::mem_pestat] mean and std.dev: (962.84, 1515.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7023) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1136.796 CPU sec, 65.324 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 659957, 49, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (202, 674, 1586) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4354) [M::mem_pestat] mean and std.dev: (552.55, 480.03) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5738) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.18, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 39, 598) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1762) [M::mem_pestat] mean and std.dev: (137.05, 307.00) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2344) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.556 CPU sec, 49.733 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660329, 53, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.27, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 46, 144) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 396) [M::mem_pestat] mean and std.dev: (43.93, 42.82) [M::mem_pestat] low and high boundaries for proper pairs: (1, 522) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.911 CPU sec, 67.347 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 660415, 40, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.15, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 48, 1090) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3234) [M::mem_pestat] mean and std.dev: (398.31, 817.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4306) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1121.757 CPU sec, 49.604 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 659613, 49, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.24, 82.51) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 40, 1777) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5303) [M::mem_pestat] mean and std.dev: (876.07, 1506.77) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7066) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1142.457 CPU sec, 66.040 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 660534, 61, 2) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (726, 1454, 3367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8649) [M::mem_pestat] mean and std.dev: (2080.17, 2144.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11290) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.09, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 38, 168) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 462) [M::mem_pestat] mean and std.dev: (44.19, 50.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 609) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.577 CPU sec, 49.950 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659966, 64, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.04, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (21, 40, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3429) [M::mem_pestat] mean and std.dev: (486.00, 881.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4565) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.973 CPU sec, 64.923 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659937, 63, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.15, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 75, 763) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2239) [M::mem_pestat] mean and std.dev: (232.08, 424.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2977) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.956 CPU sec, 50.173 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 660185, 50, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (346, 520, 1395) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3493) [M::mem_pestat] mean and std.dev: (721.33, 554.04) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4542) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.47) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 42, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (57.98, 75.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 514) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.653 CPU sec, 64.365 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660014, 46, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.21, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 29, 225) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 651) [M::mem_pestat] mean and std.dev: (49.64, 101.34) [M::mem_pestat] low and high boundaries for proper pairs: (1, 864) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1134.783 CPU sec, 50.124 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660546, 65, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.23, 82.65) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 48, 2628) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7848) [M::mem_pestat] mean and std.dev: (916.94, 1575.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10458) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1141.192 CPU sec, 66.002 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659803, 53, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.12, 82.47) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 44, 1175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3495) [M::mem_pestat] mean and std.dev: (395.20, 909.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4655) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.914 CPU sec, 49.861 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660257, 38, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.27, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 29, 72) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 192) [M::mem_pestat] mean and std.dev: (26.33, 30.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 252) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1136.525 CPU sec, 67.030 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659841, 49, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.44, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 43, 338) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 976) [M::mem_pestat] mean and std.dev: (97.37, 169.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1295) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.804 CPU sec, 50.085 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660094, 45, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 391, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.00, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 53, 1829) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5453) [M::mem_pestat] mean and std.dev: (742.49, 1246.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7265) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.568 CPU sec, 65.381 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660323, 47, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.33, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 39, 931) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2767) [M::mem_pestat] mean and std.dev: (271.83, 527.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3685) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.184 CPU sec, 49.973 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 660147, 51, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (395.95, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 31, 88) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 226) [M::mem_pestat] mean and std.dev: (29.82, 19.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 295) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.990 CPU sec, 65.169 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660263, 43, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.15, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 38, 146) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 400) [M::mem_pestat] mean and std.dev: (55.44, 73.70) [M::mem_pestat] low and high boundaries for proper pairs: (1, 527) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.973 CPU sec, 49.942 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660503, 48, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.00, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 59, 867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2571) [M::mem_pestat] mean and std.dev: (203.67, 477.35) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3423) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1123.074 CPU sec, 62.616 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 660220, 48, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.14, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 44, 1981) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5917) [M::mem_pestat] mean and std.dev: (806.89, 1367.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7885) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1117.510 CPU sec, 49.315 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659624, 51, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.16, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 45, 2509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7497) [M::mem_pestat] mean and std.dev: (729.00, 1581.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9991) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.784 CPU sec, 65.179 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660108, 58, 2) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.30, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 50, 1237) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3661) [M::mem_pestat] mean and std.dev: (343.32, 720.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4873) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.067 CPU sec, 50.037 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660148, 38, 12) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.33, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 42, 151) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 415) [M::mem_pestat] mean and std.dev: (45.16, 48.17) [M::mem_pestat] low and high boundaries for proper pairs: (1, 547) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (791, 1071, 1902) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4124) [M::mem_pestat] mean and std.dev: (998.00, 576.85) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5235) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1134.031 CPU sec, 65.038 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 659806, 46, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (260, 706, 1376) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3608) [M::mem_pestat] mean and std.dev: (619.36, 457.07) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4724) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (120, 670) [M::mem_pestat] mean and std.dev: (396.20, 82.91) [M::mem_pestat] low and high boundaries for proper pairs: (10, 780) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 32, 407) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1189) [M::mem_pestat] mean and std.dev: (98.68, 231.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1580) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.859 CPU sec, 50.167 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 659890, 49, 8) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (199, 325, 1219) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3259) [M::mem_pestat] mean and std.dev: (352.33, 329.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4279) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.31, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 72, 2602) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7778) [M::mem_pestat] mean and std.dev: (953.46, 1647.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10366) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1134.413 CPU sec, 64.316 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659745, 43, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.07, 82.70) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 41, 2689) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8033) [M::mem_pestat] mean and std.dev: (1097.98, 1776.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10705) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.828 CPU sec, 49.770 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660337, 47, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.18, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (31, 46, 223) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 607) [M::mem_pestat] mean and std.dev: (79.37, 114.02) [M::mem_pestat] low and high boundaries for proper pairs: (1, 799) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1136.567 CPU sec, 65.669 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660429, 36, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.29, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (27, 125, 1903) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5655) [M::mem_pestat] mean and std.dev: (836.17, 1112.45) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7531) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.739 CPU sec, 50.212 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 660334, 39, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (339, 1151, 1939) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5139) [M::mem_pestat] mean and std.dev: (948.22, 636.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6739) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.23, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 108, 1961) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5837) [M::mem_pestat] mean and std.dev: (849.11, 1234.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7775) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1141.025 CPU sec, 66.179 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660451, 49, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.18, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 51, 1211) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3587) [M::mem_pestat] mean and std.dev: (297.29, 520.65) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4775) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.769 CPU sec, 49.996 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659703, 38, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.20, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (22, 61, 1141) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3379) [M::mem_pestat] mean and std.dev: (501.67, 847.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4498) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1142.170 CPU sec, 65.045 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (2, 660459, 43, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.38, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 88, 1971) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5867) [M::mem_pestat] mean and std.dev: (895.52, 1351.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7815) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1131.343 CPU sec, 49.984 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 659656, 49, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (283, 447, 2565) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7129) [M::mem_pestat] mean and std.dev: (1289.64, 1326.98) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9411) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.14, 82.42) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 40, 193) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 539) [M::mem_pestat] mean and std.dev: (50.70, 71.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 712) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1136.356 CPU sec, 65.693 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659933, 44, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (322, 762, 987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2317) [M::mem_pestat] mean and std.dev: (605.22, 302.95) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2982) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 391, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.05, 82.46) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 32, 430) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1266) [M::mem_pestat] mean and std.dev: (59.71, 98.22) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1684) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1132.768 CPU sec, 50.174 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660131, 65, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.15, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 54, 1577) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4695) [M::mem_pestat] mean and std.dev: (648.45, 1106.41) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6254) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1136.037 CPU sec, 64.738 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 660269, 43, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (162, 420, 2816) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8124) [M::mem_pestat] mean and std.dev: (1761.50, 2251.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10778) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.18, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 42, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 788) [M::mem_pestat] mean and std.dev: (78.03, 135.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1043) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.621 CPU sec, 49.727 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660310, 41, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.30, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 29, 663) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1961) [M::mem_pestat] mean and std.dev: (185.56, 384.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2610) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1134.162 CPU sec, 64.795 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659681, 44, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.32, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 32, 96) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 254) [M::mem_pestat] mean and std.dev: (27.85, 18.79) [M::mem_pestat] low and high boundaries for proper pairs: (1, 333) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.390 CPU sec, 49.973 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660271, 44, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.30, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 30, 105) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 287) [M::mem_pestat] mean and std.dev: (36.50, 46.27) [M::mem_pestat] low and high boundaries for proper pairs: (1, 378) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1145.230 CPU sec, 66.773 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659639, 54, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.31, 82.45) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 35, 794) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2346) [M::mem_pestat] mean and std.dev: (228.35, 411.11) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3122) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.520 CPU sec, 49.972 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659329, 60, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 391, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (395.92, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 33, 95) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 247) [M::mem_pestat] mean and std.dev: (31.91, 27.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 323) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1137.071 CPU sec, 64.721 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 660113, 55, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (323, 401, 2052) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5510) [M::mem_pestat] mean and std.dev: (1076.08, 1203.58) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7239) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (395.94, 82.67) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 31, 1657) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4935) [M::mem_pestat] mean and std.dev: (686.60, 1313.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6574) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1130.414 CPU sec, 50.011 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659693, 53, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.08, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 34, 1744) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5202) [M::mem_pestat] mean and std.dev: (641.74, 1253.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6931) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1141.500 CPU sec, 65.711 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660099, 47, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.35, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 41, 619) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1817) [M::mem_pestat] mean and std.dev: (151.64, 359.37) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2416) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.791 CPU sec, 49.945 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 659571, 48, 3) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (314, 505, 1134) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2774) [M::mem_pestat] mean and std.dev: (697.30, 585.52) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3594) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.16, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 32, 331) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 969) [M::mem_pestat] mean and std.dev: (63.54, 144.78) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1288) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.849 CPU sec, 65.959 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659888, 51, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (395.97, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 61, 1198) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3546) [M::mem_pestat] mean and std.dev: (481.93, 743.01) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4720) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1133.920 CPU sec, 50.414 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660480, 49, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.30, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (28, 49, 1253) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3703) [M::mem_pestat] mean and std.dev: (542.27, 970.61) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4928) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.212 CPU sec, 66.116 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 659933, 50, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (312, 404, 1130) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2766) [M::mem_pestat] mean and std.dev: (666.00, 512.14) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3584) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.31, 82.53) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 27, 66) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 178) [M::mem_pestat] mean and std.dev: (23.21, 17.71) [M::mem_pestat] low and high boundaries for proper pairs: (1, 234) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (720, 1042, 1925) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4335) [M::mem_pestat] mean and std.dev: (1191.73, 698.26) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5540) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1127.144 CPU sec, 49.838 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 660704, 43, 4) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (331, 978, 1583) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4087) [M::mem_pestat] mean and std.dev: (970.80, 676.06) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5339) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.15, 82.85) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (10, 25, 1538) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4594) [M::mem_pestat] mean and std.dev: (745.73, 1435.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6487) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.258 CPU sec, 65.996 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660196, 48, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.08, 82.43) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 37, 367) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1069) [M::mem_pestat] mean and std.dev: (86.90, 176.94) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1420) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1122.626 CPU sec, 49.561 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659975, 53, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.24, 82.63) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (26, 53, 2169) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6455) [M::mem_pestat] mean and std.dev: (1026.92, 1542.88) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8598) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.200 CPU sec, 64.320 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660010, 44, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.16, 82.78) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 51, 1436) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4278) [M::mem_pestat] mean and std.dev: (280.54, 691.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5699) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1134.292 CPU sec, 50.337 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (14, 659892, 42, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (294, 383, 434) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (14, 714) [M::mem_pestat] mean and std.dev: (330.00, 82.39) [M::mem_pestat] low and high boundaries for proper pairs: (1, 854) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.06, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (15, 37, 140) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 390) [M::mem_pestat] mean and std.dev: (44.12, 57.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 515) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1138.765 CPU sec, 64.881 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (13, 660106, 63, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (278, 422, 1746) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4682) [M::mem_pestat] mean and std.dev: (742.75, 644.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6150) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (340, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (122, 667) [M::mem_pestat] mean and std.dev: (396.11, 82.72) [M::mem_pestat] low and high boundaries for proper pairs: (13, 776) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 44, 426) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1242) [M::mem_pestat] mean and std.dev: (84.50, 159.87) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1650) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.291 CPU sec, 49.725 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (15, 659994, 47, 9) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (341, 848, 3065) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 8513) [M::mem_pestat] mean and std.dev: (1952.20, 2385.60) [M::mem_pestat] low and high boundaries for proper pairs: (1, 11495) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.26, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 34, 104) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 286) [M::mem_pestat] mean and std.dev: (35.85, 33.76) [M::mem_pestat] low and high boundaries for proper pairs: (1, 377) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.309 CPU sec, 66.629 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660757, 51, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.40, 82.71) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 46, 245) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 695) [M::mem_pestat] mean and std.dev: (74.02, 112.28) [M::mem_pestat] low and high boundaries for proper pairs: (1, 920) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.668 CPU sec, 49.809 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660000, 55, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.30, 82.79) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 33, 309) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 889) [M::mem_pestat] mean and std.dev: (40.02, 54.46) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1179) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1139.971 CPU sec, 65.607 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 660694, 54, 9) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.06, 82.44) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 35, 278) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 798) [M::mem_pestat] mean and std.dev: (54.67, 124.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1058) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.968 CPU sec, 49.852 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660154, 47, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.13, 82.59) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 35, 121) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 327) [M::mem_pestat] mean and std.dev: (32.22, 26.33) [M::mem_pestat] low and high boundaries for proper pairs: (1, 430) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1132.384 CPU sec, 64.300 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (4, 660184, 60, 8) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.07, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 28, 208) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 598) [M::mem_pestat] mean and std.dev: (54.86, 85.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 793) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1127.456 CPU sec, 49.878 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660499, 54, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.00, 82.42) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 41, 686) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2024) [M::mem_pestat] mean and std.dev: (230.37, 489.43) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2693) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1129.333 CPU sec, 65.316 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660227, 37, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (453, 536, 960) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1974) [M::mem_pestat] mean and std.dev: (629.20, 281.51) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2481) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.27, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 47, 281) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 797) [M::mem_pestat] mean and std.dev: (80.61, 136.63) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1055) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.881 CPU sec, 49.612 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660050, 54, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.24, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 54, 1111) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3297) [M::mem_pestat] mean and std.dev: (419.59, 736.09) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4390) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.015 CPU sec, 64.626 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 660304, 56, 10) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.30, 82.73) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 45, 1157) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3431) [M::mem_pestat] mean and std.dev: (409.38, 771.25) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4568) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (766, 2470, 3118) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7822) [M::mem_pestat] mean and std.dev: (2229.90, 1507.86) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10174) [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::mem_process_seqs] Processed 1600000 reads in 1123.084 CPU sec, 49.616 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 659967, 43, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.31, 82.69) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 35, 545) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1607) [M::mem_pestat] mean and std.dev: (98.85, 238.62) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2138) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.510 CPU sec, 64.387 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660338, 46, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.22, 82.58) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 33, 60) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 158) [M::mem_pestat] mean and std.dev: (31.50, 28.69) [M::mem_pestat] low and high boundaries for proper pairs: (1, 207) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1125.961 CPU sec, 49.762 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 660915, 53, 11) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (692, 1115, 2708) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6740) [M::mem_pestat] mean and std.dev: (1780.67, 1751.36) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8786) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.19, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 63, 846) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2502) [M::mem_pestat] mean and std.dev: (376.42, 615.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3330) [M::mem_pestat] analyzing insert size distribution for orientation RR... [M::mem_pestat] (25, 50, 75) percentile: (928, 1369, 1867) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3745) [M::mem_pestat] mean and std.dev: (1258.30, 573.10) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4684) [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_pestat] skip orientation RR [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.608 CPU sec, 65.216 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660432, 43, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.45, 82.84) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (14, 40, 493) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1451) [M::mem_pestat] mean and std.dev: (125.50, 220.31) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1930) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.923 CPU sec, 49.790 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659661, 33, 5) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (192, 525, 2334) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6618) [M::mem_pestat] mean and std.dev: (1265.60, 1344.53) [M::mem_pestat] low and high boundaries for proper pairs: (1, 8760) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.37, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (16, 37, 2592) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 7744) [M::mem_pestat] mean and std.dev: (946.84, 1553.67) [M::mem_pestat] low and high boundaries for proper pairs: (1, 10320) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1132.689 CPU sec, 64.426 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660274, 51, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.20, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 59, 1188) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3530) [M::mem_pestat] mean and std.dev: (507.79, 884.48) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4701) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1128.626 CPU sec, 49.950 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659996, 59, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.09, 82.49) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (24, 62, 509) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1479) [M::mem_pestat] mean and std.dev: (190.69, 348.97) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1964) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.141 CPU sec, 62.888 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 659485, 54, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.19, 82.45) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 47, 1644) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4892) [M::mem_pestat] mean and std.dev: (511.47, 1124.12) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6516) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.441 CPU sec, 49.647 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660369, 54, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.08, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 40, 115) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 305) [M::mem_pestat] mean and std.dev: (36.86, 30.66) [M::mem_pestat] low and high boundaries for proper pairs: (1, 400) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1124.218 CPU sec, 62.795 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660334, 48, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.19, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 57, 1593) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4745) [M::mem_pestat] mean and std.dev: (530.81, 1082.81) [M::mem_pestat] low and high boundaries for proper pairs: (1, 6321) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1117.321 CPU sec, 49.350 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (7, 660136, 52, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.27, 82.55) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 41, 123) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 347) [M::mem_pestat] mean and std.dev: (40.48, 40.23) [M::mem_pestat] low and high boundaries for proper pairs: (1, 459) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1121.431 CPU sec, 63.834 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659735, 49, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.24, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 45, 275) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 789) [M::mem_pestat] mean and std.dev: (76.83, 127.96) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1046) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1121.846 CPU sec, 49.589 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (5, 659868, 58, 7) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.33, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (18, 42, 1155) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3429) [M::mem_pestat] mean and std.dev: (444.58, 858.72) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4566) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1135.067 CPU sec, 65.284 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (10, 659763, 59, 7) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (208, 309, 484) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1036) [M::mem_pestat] mean and std.dev: (264.50, 138.16) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1312) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.17, 82.60) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (20, 33, 732) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2156) [M::mem_pestat] mean and std.dev: (243.17, 524.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2868) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1124.670 CPU sec, 49.708 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660048, 49, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.32, 82.62) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 35, 156) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 434) [M::mem_pestat] mean and std.dev: (43.17, 54.54) [M::mem_pestat] low and high boundaries for proper pairs: (1, 573) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1133.696 CPU sec, 65.293 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 659904, 41, 6) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.18, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 42, 822) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2428) [M::mem_pestat] mean and std.dev: (225.86, 457.73) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3231) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1121.613 CPU sec, 49.448 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660383, 41, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.27, 82.66) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (23, 50, 1987) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 5915) [M::mem_pestat] mean and std.dev: (862.69, 1457.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 7879) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1123.881 CPU sec, 64.022 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (11, 660294, 40, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (332, 381, 2541) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 6959) [M::mem_pestat] mean and std.dev: (1164.64, 1113.83) [M::mem_pestat] low and high boundaries for proper pairs: (1, 9168) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.46, 82.64) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (11, 28, 1428) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 4262) [M::mem_pestat] mean and std.dev: (286.24, 631.99) [M::mem_pestat] low and high boundaries for proper pairs: (1, 5679) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1119.439 CPU sec, 49.516 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (3, 660176, 47, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (396.28, 82.68) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 37, 382) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1120) [M::mem_pestat] mean and std.dev: (73.46, 185.91) [M::mem_pestat] low and high boundaries for proper pairs: (1, 1489) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1126.316 CPU sec, 65.842 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (9, 660440, 55, 3) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.12, 82.57) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (25, 37, 1175) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 3475) [M::mem_pestat] mean and std.dev: (416.55, 833.29) [M::mem_pestat] low and high boundaries for proper pairs: (1, 4625) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.792 CPU sec, 49.661 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 660494, 42, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.09, 82.48) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (19, 48, 813) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2401) [M::mem_pestat] mean and std.dev: (301.55, 538.55) [M::mem_pestat] low and high boundaries for proper pairs: (1, 3195) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1120.811 CPU sec, 63.051 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (8, 660495, 49, 5) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.34, 82.50) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (13, 31, 73) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 193) [M::mem_pestat] mean and std.dev: (34.86, 36.21) [M::mem_pestat] low and high boundaries for proper pairs: (1, 253) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1126.117 CPU sec, 49.836 real sec [M::process] read 1600000 sequences (240000000 bp)... [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (6, 659824, 51, 4) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.29, 82.52) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (12, 37, 661) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 1959) [M::mem_pestat] mean and std.dev: (165.00, 362.56) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2608) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation RF [M::process] read 1600000 sequences (240000000 bp)... [M::mem_process_seqs] Processed 1600000 reads in 1130.876 CPU sec, 65.489 real sec [M::process] 0 single-end sequences; 1600000 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (12, 660016, 55, 6) [M::mem_pestat] analyzing insert size distribution for orientation FF... [M::mem_pestat] (25, 50, 75) percentile: (454, 721, 994) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 2074) [M::mem_pestat] mean and std.dev: (617.10, 271.30) [M::mem_pestat] low and high boundaries for proper pairs: (1, 2614) [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 392, 449) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (125, 665) [M::mem_pestat] mean and std.dev: (396.26, 82.61) [M::mem_pestat] low and high boundaries for proper pairs: (17, 773) [M::mem_pestat] analyzing insert size distribution for orientation RF... [M::mem_pestat] (25, 50, 75) percentile: (17, 47, 142) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (1, 392) [M::mem_pestat] mean and std.dev: (49.95, 66.05) [M::mem_pestat] low and high boundaries for proper pairs: (1, 517) [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_pestat] skip orientation FF [M::mem_pestat] skip orientation RF [M::mem_process_seqs] Processed 1600000 reads in 1123.723 CPU sec, 49.502 real sec [M::process] read 36216 sequences (5432400 bp)... [M::process] 0 single-end sequences; 36216 paired-end sequences [M::mem_pestat] # candidate unique pairs for (FF, FR, RF, RR): (0, 14960, 1, 1) [M::mem_pestat] skip orientation FF as there are not enough pairs [M::mem_pestat] analyzing insert size distribution for orientation FR... [M::mem_pestat] (25, 50, 75) percentile: (341, 393, 450) [M::mem_pestat] low and high boundaries for computing mean and std.dev: (123, 668) [M::mem_pestat] mean and std.dev: (397.03, 83.19) [M::mem_pestat] low and high boundaries for proper pairs: (14, 777) [M::mem_pestat] skip orientation RF as there are not enough pairs [M::mem_pestat] skip orientation RR as there are not enough pairs [M::mem_process_seqs] Processed 36216 reads in 25.527 CPU sec, 1.157 real sec [main] Version: 0.7.17-r1188 [main] CMD: /home/csyk2proj/tools/bwa.kit/bwa mem -p -t24 -R@RG\tID:KPGP-00344\tPL:ILLUMINA\tSM:KPGP-00344 -C /home/csyk2proj/data/bwa.kit/hs38DH.fa - [main] Real time: 54342.027 sec; CPU: 663284.989 sec